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1.
Chin Med J (Engl) ; 2020 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-32345819

RESUMO

BACKGROUND: Radical nephrectomy and thrombectomy is the standard surgical procedure for the treatment of renal cell carcinoma (RCC) with tumor thrombus (TT). But the estimation of intra-operative blood loss is only based on the surgeon's experience. Therefore, our study aimed to develop Peking University Third Hospital score (PKUTH score) for the prediction of intra-operative blood loss volume in radical nephrectomy and thrombectomy. METHODS: The clinical data of 153 cases of renal mass with renal vein (RV) or inferior vena cava tumor thrombus admitted to Department of Urology, Peking University Third Hospital from January 2015 to May 2018 were retrospectively analyzed. The total amount of blood loss during operation is equal to the amount of blood sucked out by the aspirator plus the amount of blood in the blood-soaked gauze. Univariate linear analysis was used to analyze risk factors for intra-operative blood loss, then significant factors were included in subsequent multivariable linear regression analysis. RESULTS: The final multivariable model included the following three factors: open operative approach (P < 0.001), Neves classification IV (P < 0.001), inferior vena cava resection (P = 0.001). The PKUTH score (0-3) was calculated according to the number of aforementioned risk factors. A significant increase of blood loss was noticed along with higher risk score. The estimated median blood loss from PKUTH score 0 to 3 was 280 mL (interquartile range [IQR] 100-600 mL), 1250 mL (IQR 575-2700 mL), 2000 mL (IQR 1250-2900 mL), and 5000 mL (IQR 4250-8000 mL), respectively. Meanwhile, the higher PKUTH score was, the more chance of post-operative complications (P = 0.004) occurred. A tendency but not significant overall survival difference was found between PKUTH risk score 0 vs. 1 to 3 (P = 0.098). CONCLUSION: We present a structured and quantitative scoring system, PKUTH score, to predict intra-operative blood loss volume in radical nephrectomy and thrombectomy.

2.
Stem Cell Res Ther ; 11(1): 113, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32169098

RESUMO

OBJECTIVES: Microvesicles (MVs) derived from human Wharton's jelly mesenchymal stem cells (MSC-MVs) were demonstrated to ameliorate acute lung injury (ALI). We have previously found that MSC-MV-transferred hepatocyte growth factor was partly involved in their therapeutic effects. Since MSC-MVs also contained a substantial quantity of miR-100, which plays an important role in lung cancer and injury, we speculated that miR-100 might similarly account for a part of the therapeutic effects of MSC-MVs. METHODS: MSCs were transfected with miR-100 inhibitor to downregulate miR-100 in MSC-MVs. A rat model of ALI and cell injury in rat type II alveolar epithelial cell line (L2) was induced by bleomycin (BLM). A co-culture model of alveolar epithelial cells and MSC-MVs was utilized to examine the therapeutic role of MSC-MVs and mechanism. RESULTS: MSC-MV treatment attenuated BLM-induced apoptosis and inflammation in BLM-treated L2 cells and ameliorated BLM-induced lung apoptosis, inflammation, and fibrosis in BLM-induced ALI rats. The beneficial effect of MSC-MVs was partly eliminated when miR-100 was knocked down in MSCs. Moreover, MSC-MV-transferred miR-100 mediated the therapeutic effect of MSC-MVs in ALI through enhancing autophagy by targeting mTOR. CONCLUSION: MSC-MVs enhance autophagy and ameliorate ALI partially via delivery of miR-100.

3.
Int J Nanomedicine ; 15: 1809-1821, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32214813

RESUMO

Introduction: Because tumor-associated inflammation is a hallmark of cancer treatment, in the present study, sorafenib mesoporous silica nanomatrix (MSNM@SFN) co-administrated with flufenamic acid (FFA, a non-steroidal anti-inflammatory drug (NSAID)) was investigated to enhance the anti-tumor activity of MSNM@SFN. Methods: Metastatic breast tumor 4T1/luc cells and hepatocellular carcinoma HepG2 cells were selected as cell models. The effects of FFA in vitro on cell migration, PGE2 secretion, and AKR1C1 and AKR1C3 levels in 4T1/luc and HepG2 cells were investigated. The in vivo anti-tumor activity of MSNM@SFN co-administrating with FFA (MSNM@SFN+FFA) was evaluated in a 4T1/luc metastatic tumor model, HepG2 tumor-bearing nude mice model, and HepG2 orthotopic tumor-bearing nude mice model, respectively. Results: The results indicated that FFA could markedly decrease cell migration, PGE2 secretion, and AKR1C1 and AKR1C3 levels in both 4T1/luc and HepG2 cells. The enhanced anti-tumor activity of MSNM@SFN+FFA compared with that of MSNM@SFN was confirmed in the 4T1/luc metastatic tumor model, HepG2 tumor-bearing nude mice model, and HepG2 orthotopic tumor-bearing nude mice model in vivo, respectively. Discussion: MSNM@SFN co-administrating with FFA (MSNM@SFN+FFA) developed in this study is an alternative strategy for improving the therapeutic efficacy of MSNM@SFN via co-administration with NSAIDs.

4.
Chin Med J (Engl) ; 132(15): 1780-1787, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31306232

RESUMO

BACKGROUND: Radical nephrectomy with thrombectomy is one of the most difficult and complicated urological operations. But the roles of renal tumor volume and thrombus level in surgical complexity and prognostic outcome are not clear. This study aimed to evaluate the surgical complexity and prognostic outcome between the volume of renal cell carcinoma (RCC) and the level of venous tumor thrombus. METHODS: The clinical data of 67 RCC cases with renal vein or inferior vena cava (IVC) tumor thrombus from January 2015 to May 2018 were retrospectively analyzed. Among these 67 cases, 21 (31.3%) were small tumors with high-level thrombus (tumor ≤7 cm in diameter and thrombus Neves Level II-IV), while 46 (68.7%) were large tumors with low-level thrombus group (tumor >7 cm in diameter and thrombus Level 0-I). Clinical features, operation details, and pathology data were collected. Univariable and multivariable logistic regression analyses were applied to evaluate the risk factors for small tumor with high-level thrombus. RESULTS: Patients with small tumors and high-level thrombus were more likely to have longer operative time (421.9 ±â€Š135.1 min vs. 282.2 ±â€Š101.9 min, t = 4.685, P < 0.001), more surgical bleeding volume (1200 [325, 2900] mL vs. 500 [180, 1000] mL, U = 270.000, P = 0.004), more surgical blood transfusion volume (800 [0, 1400] mL vs. 0 [0, 800] mL, U = 287.500, P = 0.004), more plasma transfusion volume (0 [0, 800] mL vs. 0 [0, 0] mL, U = 319.000, P = 0.004), higher percentage of open operative approach (76.2% vs. 32.6%, χ = 11.015, P = 0.001), higher percentage of IVC resection (33.3% vs. 0%, χ = 17.122, P < 0.001), and higher percentage of post-operative complications (52.4% vs. 19.6%, χ = 7.415, P = 0.010) than patients with large tumors and low-level thrombus. In multivariate analysis, decreased hemoglobin (Hb) (odds ratio [OR]: 0.956, 95% confidence interval [CI]: 0.926-0.986, P = 0.005) and non-sarcomatoid differentiation (OR: 0.050, 95% CI: 0.004-0.664, P = 0.023) were more likely to form small tumors with high-level tumor thrombus rather than large tumor with small tumor thrombus. The estimated mean cancer-specific survival times of small tumor with high-level thrombus and large tumor with low-level thrombus were 31.6 ±â€Š3.8 months and 32.5 ±â€Š2.9 months, without statistical significance (P = 0.955). After univariate and multivariate Cox proportional hazard survival regression analyses, only distant metastasis (hazard ratio [HR]: 3.839, P = 0.002), sarcomatoid differentiation (HR: 7.923, P < 0.001), alkaline phosphatase (HR: 2.661, P = 0.025), and severe post-operative complications (HR: 10.326, P = 0.001) were independent predictors of prognosis. CONCLUSIONS: The level of the tumor thrombus was more important than the diameter of the primary kidney tumor in affecting the complexity of surgery. In the same T3 stage, neither the renal tumor diameter nor the tumor thrombus level was an independent risk factor for prognosis.


Assuntos
Carcinoma de Células Renais/patologia , Carcinoma de Células Renais/cirurgia , Trombose/patologia , Trombose/cirurgia , Idoso , Feminino , Humanos , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Masculino , Pessoa de Meia-Idade , Nefrectomia , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Veia Cava Inferior/patologia , Veia Cava Inferior/cirurgia
5.
Artigo em Inglês | MEDLINE | ID: mdl-31220519

RESUMO

The level of nicotinamide adenine dinucleotide (NAD) decreases in Parkinson's disease (PD), and its reduction has been reported to be involved in many age-associated neurodegenerative pathologies. Thus, we investigated whether NAD replenishment is beneficial in a 6-hydroxydopamine (6-OHDA)-induced mouse model of PD. Preinjection with NAD in the striatum ameliorated motor deficits and dopaminergic neuronal damage in the substantia nigra and striatum of a mouse model of PD. Moreover, preincubation with NAD protected PC12 cells against the loss of cell viability, morphological damage, oxidative stress and mitochondrial dysfunction caused by 6-OHDA. These results add credence to the beneficial role of NAD against parkinsonian neurodegeneration in mouse models of PD, provide evidence for the potential of NAD for the prevention of PD, and suggest that NAD prevents pathological changes in PD via decreasing mitochondrial dysfunctions.

6.
Mol Brain ; 12(1): 23, 2019 03 25.
Artigo em Inglês | MEDLINE | ID: mdl-30909971

RESUMO

It is now generally accepted that the extra-skeleton functionalities of bone are multifaceted. Its endocrine functions came first to light when it was realized that osteoblasts, the bone forming cells, maintain energy homeostasis by improving glucose metabolism, insulin sensitivity and energy expenditure through osteocalcin, a multipurpose osteokine secreted by osteoblasts. Recently, the emerging knowledge on the functional aspects of this osteokine expanded to properties including adult and maternal regulation of cognitive functions. Therapeutic potential of this osteokine has also been recently reported in experimental Parkinson's disease models. This review highlights such findings on the functions of osteocalcin in the brain and emphasizes on exploring and analyzing much more in-depth basic and clinical studies.


Assuntos
Encéfalo/metabolismo , Cognição/fisiologia , Doença dos Neurônios Motores/metabolismo , Doença dos Neurônios Motores/fisiopatologia , Osteocalcina/metabolismo , Transdução de Sinais , Animais , Humanos , Doença dos Neurônios Motores/terapia , Fármacos Neuroprotetores/metabolismo
7.
Huan Jing Ke Xue ; 39(10): 4539-4546, 2018 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-30229601

RESUMO

Bioretention cells have become one of the most popular devices in urban stormwater runoff management for their high efficiency in reducing runoff volume and pollution loads. However, bioretention cells are unstable in nutrient pollutant removal, as is verified by many cases, especially at low temperatures. Dissolved organic matters (DOMs) plays an important role in denitrification, but the three-dimensional fluorescence characteristics of stormwater runoff in bioretention field cells are poorly understood, so to improve the performance of bioretention cells in nutrient pollutants control, the authors determined that it was necessary to clarify the characteristics of three-dimensional fluorescence of stormwater runoff from bioretention cells and explain their relationship to water quality. To be applicable to urban landuse in mountainous cities, two field lined bioretention cells were constructed, and, from March to April in 2017, while temperatures were still low, water quality and three-dimensional fluorescence of stormwater runoff were analyzed during low rainfall intensity precipitation events. Analysis results showed that the effluent pollutant concentrations had low fluctuation after purification by the bioretention cells, and both cells showed relatively stable removal rates for NH4+-N and total phosphorus (TP; i. e., 78.38%-95.03% and 72.04%-76.04%, respectively). Analysis of fluorescence spectra showed that the DOMs in the runoff from the two cells was mostly made up of protein and humic substances, mainly of biological origin or aquatic bacterial metabolites. Both cells performed well in the removal of protein at I, protein at Ⅱ and fulvic acid (i. e., the removal rates could reach 57.33%-61.30%, 29.82%-31.28%, and 35.55%-43.16% respectively). Correlation analyses between water quality and DOM showed that total Nitrogen (TN), TP and total organic carbon (TOC) were positively correlated with the protein content in runoff from the biorentention cells, while NO3--N and NH4+-N were negatively correlated with protein content. Meanwhile, TN was negatively correlated with DOMs in regions Ⅳ and Ⅴ of the cells.


Assuntos
Chuva , Movimentos da Água , Poluentes Químicos da Água/isolamento & purificação , Qualidade da Água , Bactérias , Cidades , Monitoramento Ambiental , Fluorescência , Nitrogênio/isolamento & purificação , Fósforo/isolamento & purificação
8.
Environ Sci Pollut Res Int ; 25(15): 15006-15018, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29552716

RESUMO

A growing body of evidence has shown bisphenol A (BPA), an estrogen-like industrial chemical, has adverse effects on the nervous system. In this study, we investigated the transcriptional behavior of long non-coding RNAs (lncRNAs) and mRNAs to provide the information to explore neurotoxic effects induced by BPA. By microarray expression profiling, we discovered 151 differentially expressed lncRNAs and 794 differentially expressed mRNAs in the BPA intervention group compared with the control group. Gene ontology analysis indicated the differentially expressed mRNAs were mainly involved in fundamental metabolic processes and physiological and pathological conditions, such as development, synaptic transmission, homeostasis, injury, and neuroinflammation responses. In the expression network of the BPA-induced group, a great number of nodes and connections were found in comparison to the control-derived network. We identified lncRNAs that were aberrantly expressed in the BPA group, among which, growth arrest specific 5 (GAS5) might participate in the BPA-induced neurotoxicity by regulating Jun, RAS, and other pathways indirectly through these differentially expressed genes. This study provides the first investigation of genome-wide lncRNA expression and correlation between lncRNA and mRNA expression in the BPA-induced neurotoxicity. Our results suggest that the elevated expression of lncRNAs is a major biomarker in the neurotoxicity induced by BPA.


Assuntos
Compostos Benzidrílicos/toxicidade , Neurotoxinas/toxicidade , Fenóis/toxicidade , RNA Longo não Codificante/metabolismo , Animais , Perfilação da Expressão Gênica , Ontologia Genética , Redes Reguladoras de Genes , Análise de Sequência com Séries de Oligonucleotídeos , Células PC12 , RNA Mensageiro/metabolismo , Ratos , Análise Serial de Tecidos
9.
Nat Commun ; 9(1): 474, 2018 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-29382837

RESUMO

The original version of this Article contained an error in Fig. 6b. In the top scattering process, while the positioning of both arrows was correct, the colours were switched: the first arrow was red and the second arrow was blue, rather than the correct order of blue then red.

10.
Nat Commun ; 9(1): 137, 2018 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-29305576

RESUMO

The original version of this Article omitted the following from the Acknowledgements:"This work was partly supported by the Research Council of Norway through its Centres of Excellence funding scheme, project number 262633, QuSpin."This has now been corrected in both the PDF and HTML versions of the article.

11.
Nat Commun ; 8(1): 2019, 2017 12 08.
Artigo em Inglês | MEDLINE | ID: mdl-29222507

RESUMO

Topological superconductivity is central to a variety of novel phenomena involving the interplay between topologically ordered phases and broken-symmetry states. The key ingredient is an unconventional order parameter, with an orbital component containing a chiral p x + ip y wave term. Here we present phase-sensitive measurements, based on the quantum interference in nanoscale Josephson junctions, realized by using Bi2Te3 topological insulator. We demonstrate that the induced superconductivity is unconventional and consistent with a sign-changing order parameter, such as a chiral p x + ip y component. The magnetic field pattern of the junctions shows a dip at zero externally applied magnetic field, which is an incontrovertible signature of the simultaneous existence of 0 and π coupling within the junction, inherent to a non trivial order parameter phase. The nano-textured morphology of the Bi2Te3 flakes, and the dramatic role played by thermal strain are the surprising key factors for the display of an unconventional induced order parameter.

12.
J Pineal Res ; 63(3)2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28580724

RESUMO

Retinitis pigmentosa (RP) comprises a group of incurable inherited retinal degenerations. Targeting common processes, instead of mutation-specific treatment, has proven to be an innovative strategy to combat debilitating retinal degeneration. Growing evidence indicates that melatonin possesses a potent activity against neurodegenerative disorders by mitigating cell damage associated with apoptosis and inflammation. Given the pleiotropic role of melatonin in central nervous system, the aim of the present study was to investigate whether melatonin would afford protection against retinal degeneration in autosomal recessive RP (arRP). Rd10, a well-characterized murine model of human arRP, received daily intraperitoneal injection of melatonin (15 mg/kg) between postnatal day (P) 13 and P30. Retinas treated with melatonin or vehicle were harvested for analysis at P30 and P45, respectively. The findings showed that melatonin could dampen the photoreceptors death and delay consequent retinal degeneration. We also observed that melatonin weakened the expression of glial fibrillary acidic protein (GFAP) in Müller cells. Additionally, melatonin could alleviate retinal inflammatory response visualized by IBA1 staining, which was further corroborated by downregulation of inflammation-related genes, such as tumor necrosis factor alpha (Tnf-α), chemokine (C-C motif) ligand 2 (Ccl2), and chemokine (C-X-C motif) ligand 10 (Cxcl10). These data revealed that melatonin could ameliorate retinal degeneration through potentially attenuating apoptosis, reactive gliosis, and microglial activation in rd10 mice. Moreover, these results suggest melatonin as a promising agent improving photoreceptors survival in human RP.


Assuntos
Antioxidantes/uso terapêutico , Melatonina/uso terapêutico , Células Fotorreceptoras de Vertebrados/efeitos dos fármacos , Retinite Pigmentosa/tratamento farmacológico , Animais , Antioxidantes/farmacologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Células Ependimogliais/efeitos dos fármacos , Gliose/prevenção & controle , Melatonina/farmacologia , Camundongos , Microglia/efeitos dos fármacos
13.
Medicine (Baltimore) ; 96(19): e6902, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28489803

RESUMO

Shear Wave Elastography (SWE) is an objective and non-invasive method widely used to quantify the tissue solidity. However, there are concerns about the accuracy of the skin SWE results due to the low signal-to-noise ratio (SNR) caused by subcutaneous fat, muscle and bone. This article analyzed the reproducibility of the result for skin SWE and therefore evaluated the availability of SME for skin elasticity involved diseases. Thirty volunteers (mean age: 37 ±â€Š12 years) were selected. SWE were taken on the skin of abdomen and the middle tibia in order to assess the impact of fat, muscle and bone on SWE results. Skin in the area of anterior and lateral tibia marked with seven parallel lines, and each line indicated an identical thickness of the subcutaneous fat from 1-7 mm. Intra-class correlation coefficients (ICC) were used to evaluate the intra-observer and inter-observer reproducibility. The solidity of abdominal skin showed soft and small individual differences (12.4 ±â€Š2.7 kPa), whereas high shear moduli (25-48 kPa) were observed in the skin above tibia and tibialis anterior muscle. When the subcutaneous fat was thicker than 3 mm (≥3), we obtained excellent intra-observer reproducibility (ICC range 0.78-0.98) and inter-observer reproducibility (ICC range 0.75-0.98). The thickness of subcutaneous fat could affect the reproducibility of skin SWE. The further study on skin SWE standardization should be taken.


Assuntos
Técnicas de Imagem por Elasticidade , Elasticidade , Fenômenos Fisiológicos da Pele , Abdome/fisiologia , Adulto , Composição Corporal , Feminino , Humanos , Masculino , Músculo Esquelético/fisiologia , Variações Dependentes do Observador , Tamanho do Órgão , Estudos Prospectivos , Reprodutibilidade dos Testes , Gordura Subcutânea/anatomia & histologia , Gordura Subcutânea/fisiologia , Tíbia/fisiologia
14.
Abdom Radiol (NY) ; 42(3): 802-809, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-27761613

RESUMO

OBJECTIVES: To evaluate the diagnostic performance of contrast-enhanced ultrasound (CEUS) in differential diagnosis of gastric cancer and gastritis, with histological results as reference standard. METHODS: From September 2011 to August 2014, 82 patients (50 males and 32 females; mean age ± SD, 59.5 ± 15.0 years; range 19-91 years) with gastric cancer or gastritis were included in this Ethics Committee-approved prospective study. Conventional ultrasonography (US) and CEUS were applied to distinguish the two lesions, and both qualitative and quantitative features were evaluated. RESULTS: Of the 82 histopathologic-proven lesions, 58 were cancer and 24 were gastritis. For US, the gastric wall stratification was not preserved in about one-third of cancer (21/58, 36.2%) compared with gastritis (0/24, 0%) (p < 0.001). Blurred, angular, or spiculated serosa margin and increased echogenicity in perigastric fat appeared only in cancer (10/58, 17.2%), and all of them proved to be pathologic T3 or T4 stage. On CEUS, gastric cancer usually manifested as diffused enhancement without comb-teeth-like vessels (parallel curvilinear structures representing arterial branching within the gastric wall) (56/58, 96.6%), while these vessels presented in most gastritis (19/24, 79.2%, p < 0.001). For quantitative analysis, the malignant lesions showed later and lower enhancement (p < 0.001), and they also had slower speed to reach the peak intensity (p < 0.001). On CEUS, the absence of comb-teeth-like vessel is most reliable for diagnosing malignancy, and the sensitivity, specificity, and accuracy were 96.5%, 79.2%, and 91.5%, respectively. CONCLUSIONS: Our results demonstrated the usefulness and accuracy of US and CEUS in differential diagnosis of gastric cancer and gastritis. CEUS has the potential to make the diagnosis more accurate.


Assuntos
Gastrite/diagnóstico por imagem , Neoplasias Gástricas/diagnóstico por imagem , Ultrassonografia Doppler em Cores/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Meios de Contraste , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fosfolipídeos , Sensibilidade e Especificidade , Hexafluoreto de Enxofre
15.
Huan Jing Ke Xue ; 38(2): 469-475, 2017 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964501

RESUMO

Urban traffic is an important source of greenhouse gases such as CH4. The observations on CH4 are the basis for quantitative analysis of urban carbon emissions. Taken into consideration the weekly and daily changing characteristics of urban traffic, we conducted experiments to analyze the features of traffic CH4emission and its influential factors. The experiments were conducted on 3 main roads in Nanjing on Oct. 17, 18, 20, 23, 2014 with 5 periods of observation per day, and in Nanjing Yangtze River tunnel in the morning and at night of Sep.11 2014. The results showed that:① The average concentration of CH4 on the urban main road of Nanjing city was greater than that of the background atmosphere. Affected by traffic conditions, the spatial difference of ΔCH4 concentration was significant on three typical main roads. ΔCH4 concentration's diurnal variation showed inverted "W" type, and its peak appeared in the morning and evening rush hours. ② Due to the "piston wind" in the tunnel, the CH4 concentration in Nanjing Yangtze River tunnel gradually increased from the inlet to the outlet and the difference of concentration between the inlet and the outlet was 0.21×10-6-0.38×10-6. ③ There was a good linear correlation between CH4 concentration and CO2 concentration. The atmospheric ΔCH4:ΔCO2 value of urban main road in Nanjing was 0.0091 and the atmospheric ΔCH4:ΔCO2 value of Nanjing Yangtze River Tunnel was 0.00047-0.0014. ④ Traffic volume and the proportion of natural gas vehicles were the main factors influencing atmospheric ΔCH4 concentration and ΔCH4:ΔCO2.

16.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 38(3): 327-30, 2016 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-27469920

RESUMO

Objective To evaluate the value of high-frequency ultrasound (HFUS) in diagnosing peripheral nerve compression in patients with rheumatoid arthritis (RA). Methods The upper limb nerves were evaluated by HFUS in 80 RA patients (RA group) and 60 non-RA patients (control group),then the incidence of peripheral nerve compression was recorded respectively. RA patients with/without neurological symptoms were compared in terms of age,disease course,Health Assessment Questionnaire Disability Index (HAQ-DI) score,and clinical disease activity index (CDAI). Results The incidence of upper limb nerve compression in RA group was significantly higher than that in control group(15.0% vs. 3.3%,P=0.046).The patients with nerve compression was older [(60.2±11.4)y vs.(49.2±7.9)y;t=2.343,P=0.039] and had longer disease course [(9.50±5.99) y vs. (5.88±3.87)y;t=2.639,P=0.023] and higher HAQ-DI score (1.58±0.75 vs.0.85±0.67;t=2.490,P=0.030). These two groups had no statistical differences in CDAI (14.50±11.68 vs.16.62±9.24;t=1.141,P=0.278).Conclusions Peripheral neuropathies are common extra-articular manifestations in RA patients. HFUS can be valuable in patients suspected of RA.


Assuntos
Artrite Reumatoide/diagnóstico por imagem , Síndromes de Compressão Nervosa/diagnóstico por imagem , Adulto , Progressão da Doença , Humanos , Pessoa de Meia-Idade , Ultrassonografia
17.
Huan Jing Ke Xue ; 36(7): 2367-73, 2015 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-26489300

RESUMO

Direct observation of urban atmospheric CO2 concentration is vital for the research in the contribution of anthropogenic activity to the atmospheric abundance since cities are important CO2 sources. The observations of the atmospheric CO2 concentration at multiple sites/heights can help us learn more about the temporal and spatial patterns and influencing mechanisms. In this study, the CO2 concentration was observed at 5 sites (east, west, south, north and middle) in the main city area of Nanjing from July 18 to 25, 2014, and the vertical profile of atmospheric CO2 concentration was measured in the middle site at 3 heights (30 m, 65 m and 110 m). The results indicated that: (1) An obvious vertical CO2 gradient was found, with higher CO2 concentration [molar fraction of 427. 3 x 10(-6) (±18. 2 x 10(-6))] in the lower layer due to the strong influences of anthropogenic emissions, and lower CO2 concentration in the upper layers [411. 8 x 10(-6) (±15. 0 x 10(-6)) and 410. 9 x 10(-6) (±14. 6 x 10(-6)) at 65 and 110 m respectively] for the well-mixed condition. The CO2 concentration was higher and the vertical gradient was larger when the atmosphere was stable. (2) The spatial distribution pattern of CO2 concentration was dominated by wind and atmospheric stability. During the observation, the CO2 concentration in the southwest was higher than that in the northeast region with the CO2 concentration difference of 7. 8 x 10(-6), because the northwest wind was prevalent. And the CO2 concentration difference reduced with increasing wind speed since stronger wind diluted CO2 more efficiently. The more stable the atmosphere was, the higher the CO2 concentration was. (3) An obvious diurnal variation of CO2 concentration was shown in the 5 sites. A peak value occurred during the morning rush hours, the valley value occurred around 17:00 (Local time) and another high value occurred around 19:00 because of evening rush hour sometimes.


Assuntos
Poluentes Atmosféricos/análise , Atmosfera/análise , Dióxido de Carbono/análise , Monitoramento Ambiental , Estações do Ano , China , Cidades , Vento
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 35(5): 1357-64, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26415460

RESUMO

With the development of remote sensing technology and imaging spectrometer, the resolution of hyperspectral remote sensing image has been continually improved, its vast amount of data not only improves the ability of the remote sensing detection but also brings great difficulties for analyzing and processing at the same time. Band selection of hyperspectral imagery can effectively reduce data redundancy and improve classification accuracy and efficiency. So how to select the optimum band combination from hundreds of bands of hyperspectral images is a key issue. In order to solve these problems, we use spectral clustering algorithm based on graph theory. Firstly, taking of the original hyperspectral image bands as data points to be clustered , mutual information between every two bands is calculated to generate the similarity matrix. Then according to the graph partition theory, spectral decomposition of the non-normalized Laplacian matrix generated by the similarity matrix is used to get the clusters, which the similarity between is small and the similarity within is large. In order to achieve the purpose of dimensionality reduction, the inter-class separability factor of feature types on each band is calculated, which is as the reference index to choose the representative bands in the clusters furthermore. Finally, the support vector machine and minimum distance classification methods are employed to classify the hyperspectral image after band selection. The method in this paper is different from the traditional unsupervised clustering method, we employ spectral clustering algorithm based on graph theory and compute the interclass separability factor based on a priori knowledge to select bands. Comparing with traditional adaptive band selection algorithm and band index based on automatically subspace divided algorithm, the two sets of experiments results show that the overall accuracy of SVM is about 94. 08% and 94. 24% and the overall accuracy of MDC is about 87. 98% and 89. 09%, when the band selection achieves a relatively optimal number of clusters using the method propoesd in this paper. It effectively remains spectral information and improves the classification accuracy.

19.
PLoS One ; 10(6): e0130382, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26076347

RESUMO

Assisted reproductive technology (ART) is being increasingly applied to overcome infertility. However, the in vitro production process, the main procedure of ART, can lead to aberrant embryonic development and health-related problems in offspring. Understanding the mechanisms underlying the ART-induced side effects is important to improve the ART process. In this study, we carried out comparative transcriptome profiling between in vivo- (IVO) and in vitro- produced (IVP) mouse blastocysts. Our results suggested that aberrant actin organization might be a major factor contributing to the impaired development of IVP embryos. To test this, we examined the effect of actin disorganization on the development of IVP preimplantation embryos. Specific disruption of actin organization by cytochalasin B (CB) indicated that well-organized actin is essential for in vitro embryonic development. Supplementing the culture medium with 10(-9) M melatonin, a cytoskeletal modulator in adult somatic cells, significantly reversed the disrupted expression patterns of genes related to actin organization, including Arhgef2, Bcl2, Coro2b, Flnc, and Palld. Immunofluorescence analysis showed that melatonin treatment of IVP embryos significantly improved the distribution and organization of actin filaments (F-actin) from the 8-cell stage onwards. More importantly, we found that melatonin alleviated the CB-mediated aberrant F-actin distribution and organization and rescued CB-induced impaired embryonic development. This is the first study to indicate that actin disorganization is implicated in impaired development of IVP embryos during the preimplantation stage. We also demonstrated that improving actin organization is a promising strategy to optimize existing IVP systems.


Assuntos
Actinas/genética , Blastocisto/citologia , Citocalasina B/farmacologia , Desenvolvimento Embrionário/efeitos dos fármacos , Melatonina/farmacologia , Actinas/biossíntese , Animais , Sequência de Bases , Técnicas de Cultura Embrionária , Embrião de Mamíferos/metabolismo , Feminino , Fertilização In Vitro , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Masculino , Camundongos , Camundongos Endogâmicos ICR , Análise de Sequência de RNA
20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 35(2): 557-62, 2015 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-25970932

RESUMO

As the rotation speed of ground based hyperspectral imaging system is too fast in the image collection process, which exceeds the speed limitation, there is data missed in the rectified image, it shows as the_black lines. At the same time, there is serious distortion in the collected raw images, which effects the feature information classification and identification. To solve these problems, in this paper, we introduce the each component of the ground based hyperspectral imaging system at first, and give the general process of data collection. The rotation speed is controlled in data collection process, according to the image cover area of each frame and the image collection speed of the ground based hyperspectral imaging system, And then the spatial orientation model is deduced in detail combining with the star scanning angle, stop scanning angle and the minimum distance between the sensor and the scanned object etc. The oriented image is divided into grids and resampled with new spectral. The general flow of distortion image corrected is presented in this paper. Since the image spatial resolution is different between the adjacent frames, and in order to keep the highest image resolution of corrected image, the minimum ground sampling distance is employed as the grid unit to divide the geo-referenced image. Taking the spectral distortion into account caused by direct sampling method when the new uniform grids and the old uneven grids are superimposed to take the pixel value, the precise spectral sampling method based on the position distribution is proposed. The distortion image collected in Lao Si Cheng ruin which is in the Zhang Jiajie town Hunan province is corrected through the algorithm proposed on above. The features keep the original geometric characteristics. It verifies the validity of the algorithm. And we extract the spectral of different features to compute the correlation coefficient. The results show that the improved spectral sampling method is better than the direct sampling method. It provides the reference for the similar product used on the ground.

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