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1.
Biosci Rep ; 40(3)2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-32091104

RESUMO

Acute lung injury (ALI) is a lethal disease with diffuse lung inflammation, in which JAK/STAT3 signaling has been well recognized for its role in initiating and amplifying inflammatory processes. However, the mechanism for the enhancement and maintenance of signal transducer and activator of transcription 3 (STAT3) activation has not yet been clearly demonstrated in ALI. In the present work, we established a lipopolysaccharide (LPS)-induced ALI rat model through intratracheal instillation and isolated the alveolar macrophages (AMs) from the rats in the model. We demonstrated that the expression of Kruppel-like factor 2 (KLF2) significantly decreased in the AMs from LPS-induced ALI rats (LPS-AMs) as compared with the AMs from control rats (NC-AMs). Overexpressing KLF2 in LPS-AMs inhibited the phosphorylation of STAT3 and reduced the levels of STAT3 target genes, including matrix metalloproteinase (MMP)-2/9 (MMP-2/9). Further investigation indicated that KLF2 trans-inhibited heat shock protein H1 (HSPH1), which interacted with STAT3 and enhanced its phosphorylation. As a crucial inflammatory mediator in ALI, interleukin-1ß (IL-1ß) induced the down-regulation of KLF2 in LPS-AMs, as interrupting IL-1ß signaling in LPS-AMs by antibody neutralization or IL1R1 knockdown rescued the expression of KLF2. Consistently, stimulating NC-AMs with IL-1ß decreased KLF2 and increased HSPH1, while overexpression of KLF2 suppressed IL-1ß-induced HSPH1. Additionally, in vivo studies showed that treatment with an IL-1ß antibody or HSPH1 inhibitor alleviated lung injury in ALI rats, as well as decreased the levels of p-STAT3 and MMP-2/9. In conclusion, activation of the IL-1ß/KLF2/HSPH1 pathway facilitated STAT3 phosphorylation in AMs, which exacerbated pulmonary inflammation in ALI.

2.
Int J Mol Sci ; 21(4)2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32093294

RESUMO

BAK1 (brassinosteroid-insensitive 1 (BRI1) associated receptor kinase 1) plays major roles in multiple signaling pathways as a coreceptor to regulate plant growth and development and stress response. However, the role of BAK1 in high light signaling is still poorly understood. Here we observed that overexpression of BAK1 in Arabidopsis interferes with the function of high light in promoting plant growth and development, which is independent of the brassinosteroid (BR) signaling pathway. Further investigation shows that high light enhances the phosphorylation of BAK1 and catalase activity, thereby reducing hydrogen peroxide (H2O2) accumulation. Catalase3 (CAT3) is identified as a BAK1-interacting protein by affinity purification and LC-MS/MS analysis. Biochemical analysis confirms that BAK1 interacts with and phosphorylates all three catalases (CAT1, CAT2, and CAT3) of the Arabidopsis genome, and the trans-phosphorylation sites of three catalases with BAK1-CD are identified by LC-MS/MS in vitro. Genetic analyses reveal that the BAK1 overexpression plants knocked out all the three CAT genes completely abolishing the effect of BAK1 on suppression of high light-promoted growth. This study first unravels the role of BAK1 in mediating high light-triggered activation of CATs, thereby degrading H2O2 and regulating plant growth and development in Arabidopsis.

3.
Medicine (Baltimore) ; 99(6): e19004, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32028411

RESUMO

BACKGROUND: Endoscopic tonsillectomy is associated with postoperative pain. Postoperative pain management remains to be improved in children. We aimed to investigate oxycodone preemptive analgesia in children undergoing endoscopic plasma total adenotonsillectomy. METHODS: 166 children with adenotonsillar hypertrophy were recruited at Wuhan Children's Hospital between 08/2016 and 03/2017. They were randomly assigned to receive SPOA (postoperative sufentanil), SPEA+SPOA (preemptive sufentanil and postoperative sufentanil), and OPEA+SPOA (preemptive oxycodone and postoperative sufentanil). The primary endpoint was serum c-fos levels. The secondary endpoints were the response entropy (RE) value, Pediatric Anesthesia Emergence Delirium (PAED) score, FLACC score, and adverse events. RESULTS: c-fos mRNA levels were increased significantly after surgery in the SPOA and SPEA+SPOA groups (P < .05). Postoperatively, c-fos mRNA levels were higher in the SPOA group compared with the OPEA+SPOA group (P = .044). The RE values increased in all groups after surgery (P < .05). At extubation, RE values were higher in the SPOA group compared with the SPEA+SPOA and OPEA+SPOA groups (P < .05). The PAED scores were higher in the SPOA group compared with the OPEA+SPOA group (P = .045). In the SPOA group, the FLACC scores were decreased at 24 h after surgery vs 4 hours (P = .044). Prediction probability (Pk) values indicated that RE and c-fos mRNA levels were quantitative predictors for early postoperative stress reaction after surgery. CONCLUSIONS: The subanalgesic dose of oxycodone (0.1 mg/kg) as preemptive analgesia could improve pain after endoscopic plasma total adenotonsillectomy in children.


Assuntos
Adenoidectomia , Analgésicos Opioides/uso terapêutico , Oxicodona/uso terapêutico , Dor Pós-Operatória/prevenção & controle , Tonsilectomia , Actinas/sangue , Adenoidectomia/efeitos adversos , Adenoidectomia/métodos , Criança , Endoscopia/efeitos adversos , Endoscopia/métodos , Feminino , Humanos , Masculino , Proteínas Proto-Oncogênicas c-fos/sangue , Reação em Cadeia da Polimerase em Tempo Real , Sufentanil/uso terapêutico , Tonsilectomia/efeitos adversos , Tonsilectomia/métodos
4.
Haematologica ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601687

RESUMO

Transferrin receptor 1 (Tfr1) mediates the endocytosis of diferric transferrin in order to transport iron, and Tfr1 has been suggested to play an important role in hematopoiesis. To study the role of Tfr1 in hematopoiesis, we generated hematopoietic stem cell (HSC) specific Tfr1 knockout mice (Tfr1-CKO). We found that Tfr1-CKO mice reached full term but died within one week of birth. Further analyses revealed that Tfr1-deficient HSCs had impaired development of all hematopoietic progenitors except thrombocytes and B lymphocytes. In addition, Tfr1-deficient cells had cellular iron deficiency, which blocked the proliferation and differentiation of hematopoietic precursor cells, attenuated the commitment of hematopoietic lineages, and reduced the regeneration potential of HSCs. Notably, hemin rescued the colony-forming capacity of Tfr1-deficient HSCs, whereas expressing a mutant Tfr1 that lack the protein's iron-transporting capacity failed to rescue hematopoiesis. These findings provide direct evidence that Tfr1 is essential for hematopoiesis through binding diferric transferrin to supply iron to cells.

5.
J Cancer ; 10(17): 3908-3913, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31417634

RESUMO

Backgroud: Increasing studies show that circular RNAs (circRNAs) play important roles in tumor progression. However, the function of circRNAs in ovarian cancer is mostly unclear. Methods: We detected the expression of circGFRA1 by quantitative real-time PCR (qRT-PCR) in 50 pairs of ovarian cancer tissues and adjacent normal tissues. Then, we explored the function of circGFRA1 in ovarian cancer progression, such as cell proliferation, apoptosis and invasion. Moreover, we performed luciferase reporter and RNA immunoprecipitation (RIP) assay to study the competing endogenous RNA (ceRNA) function of circGFRA1 in ovarian cancer progression. Results: qRT-PCR showed that circGFRA1 was overexpressed in ovarian cancer tissues. Inhibition of circGFRA1 suppressed cell proliferation and invasion, but induced cell apoptosis in ovarian cancer. Luciferase reporter and RIP assay revealed that circGFRA1 could regulate the expression of GFRA1 by sponging miR-449a. Conclusions: In summary, circGFRA1 regulated GFRA1 expression and ovarian cancer progression by sponging miR-449a. circGFRA1 could be a potential diagnostic biomarker and therapeutic target for ovarian cancer.

6.
Phys Chem Chem Phys ; 21(40): 22203-22214, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31432826

RESUMO

The N+(3P) + H2(X1Σ) → NH+(X2Π) + H(2S) reaction is important for initiating the chain reaction of ammonia synthesis in the universe. To study the dynamics of this reaction, a global accurate potential energy surface (PES) of the ground state NH was constructed by combining numerous high-level ab initio energy points with the permutation invariant polynomial neural network method. Utilizing this newly constructed PES, time-dependent wave packet calculations were performed on the state-to-state reactions of N+(3P0) + H2 (v = 0, j = 0) and N+(3P0) + D2 (v = 0, j = 0) in order to study the microscopic reaction mechanisms and dynamical isotope effects. Isotope effects have a significant influence on the rovibrational state distributions and state resolved angle distributions of the product. The total differential cross sections (DCSs) present in the aforementioned reactions are dominated by both forward and backward scattering, as expected from the observable deep well along the reaction path. Meanwhile, the rovibrational state-resolved DCSs show that both reactions are not entirely statistical at the state-to-state level.

7.
BMC Plant Biol ; 19(1): 256, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31196007

RESUMO

BACKGROUND: Appropriate brassinosteroid (BR) signal strength caused by exogenous application or endogenous regulation of BR-related genes can increase crop yield. However, precise control of BR signals is difficult and can cause unstable effects and failure to reach full potential. Phosphorylated BRASSINOSTEROID INSENSITIVE1 (BRI1), the rate-limiting receptor in BR signalling, transduces BR signals, and we recently demonstrated that modifying BRI1 phosphorylation sites alters BR signal strength and botanical characteristics in Arabidopsis. However, the functions of such phosphorylation sites in agronomic characteristics of crops remain unclear. RESULTS: In this work, we investigated the roles of tomato SlBRI1 threonine-1050 (Thr-1050). SlBRI1 mutant cu3-abs1 plants expressing SlBRI1 with a non-phosphorylatable Thr-1050 (T1050A), with a wild-type SlBRI1 transformant used as a control, were examined. The results showed enhanced autophosphorylation of SlBRI1 and BR signal strength for cu3-abs1 harbouring T1050A, which promoted yield through increased plant expansion, leaf area, fruit weight and fruit number per cluster but reduced nutrient contents, including ascorbic acid and soluble sugar levels. Moreover, plant height, stem diameter, and internodal distance were similar between the transgenic plants. CONCLUSION: Our results reveal the biological role of Thr-1050 in tomato and provide a molecular basis for establishing high-yield crops by precisely controlling BR signal strength via phosphorylation site modification.


Assuntos
Brassinosteroides/metabolismo , Frutas/crescimento & desenvolvimento , Lycopersicon esculentum/crescimento & desenvolvimento , Proteínas de Plantas/fisiologia , Proteínas Quinases/fisiologia , Transdução de Sinais , Lycopersicon esculentum/genética , Mutação , Fosforilação , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Proteínas Quinases/genética , Proteínas Quinases/metabolismo
8.
J Transl Med ; 17(1): 58, 2019 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-30813939

RESUMO

BACKGROUND: Progesterone resistance is a problem in endometrial carcinoma, and its underlying molecular mechanisms remain poorly understood. The aim of this study was to elucidate the molecular mechanisms of progesterone resistance and to identify the key genes and pathways mediating progesterone resistance in endometrial cancer using bioinformatics analysis. METHODS: We developed a stable MPA (medroxyprogesterone acetate)-resistant endometrial cancer cell subline named IshikawaPR. Microarray analysis was used to identify differentially expressed genes (DEGs) from triplicate samples of Ishikawa and IshikawaPR cells. PANTHER, DAVID and Metascape were used to perform gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and cBioPortal for progesterone receptor (PGR) coexpression analysis. GEO microarray (GSE17025) was utilized for validation. The protein-protein interaction network (PPI) and modular analyses were performed using Metascape and Cytoscape. Further validation were performed by real-time polymerase chain reaction (RT-PCR). RESULTS: In total, 821 DEGs were found and further analyzed by GO, KEGG pathway enrichment and PPI analyses. We found that lipid metabolism, immune system and inflammation, extracellular environment-related processes and pathways accounted for a significant portion of the enriched terms. PGR coexpression analysis revealed 7 PGR coexpressed genes (ANO1, SOX17, CGNL1, DACH1, RUNDC3B, SH3YL1 and CRISPLD1) that were also dramatically changed in IshikawaPR cells. Kaplan-Meier survival statistics revealed clinical significance for 4 out of 7 target genes. Furthermore, 8 hub genes and 4 molecular complex detections (MCODEs) were identified. CONCLUSIONS: Using microarray and bioinformatics analyses, we identified DEGs and determined a comprehensive gene network of progesterone resistance. We offered several possible mechanisms of progesterone resistance and identified therapeutic and prognostic targets of progesterone resistance in endometrial cancer.

9.
Anal Chim Acta ; 1061: 122-133, 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-30926030

RESUMO

A facile and efficient method for the determination of triazine herbicides in tomato samples was developed by employing 3-aminophenol-glyoxylic acid resin microspheres as a solid-phase extraction adsorbent followed by high performance liquid chromatography analysis. These resin microspheres were synthesized by a simple green precipitation polymerization method, and a range of functional groups (hydroxyl, amino, carboxylic group) were introduced through the 3-aminophenol and glyoxylic acid components. The as-prepared resin microspheres were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, and thermal gravimetric analyzer. The resin microspheres exhibited a good adsorption rate, large adsorption amount, and short adsorption equilibrium time (almost in ∼5 min). Under the optimal extraction and determination conditions, a good linearity was obtained in the range of 0.025-7.5 µg g-1 (r2 ≥ 0.9997) for atraton, ametryn, and prometryn. The limits of detection of atraton, ametryn, and prometryn were 0.57, 0.75, and 1.06 µg kg-1, respectively. The intra-day and inter-day precisions expressed as relative standard deviations were in the ranges of 1.8-3.2% and 1.7-4.1%, respectively. In addition, the recoveries at three spiked levels ranged from 85.1 to 97.7% with the relative standard deviation ≤ 5.4% (n = 3). This novel method is simple and accurate and has proved to be a reliable alternative method for the determination of triazine herbicides in tomato samples.


Assuntos
Aminofenóis/química , Contaminação de Alimentos/análise , Glioxilatos/química , Herbicidas/análise , Lycopersicon esculentum/química , Triazinas/análise
10.
J Cell Mol Med ; 23(5): 3597-3602, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30887698

RESUMO

Increasing studies show that circular RNAs (circRNAs) play vital roles in tumour progression. But, how circRNAs function in ovarian cancer is mostly unclear. Here, we detected the expression of circEPSTI1 in ovarian cancer and explored the function of circEPSTI1 in ovarian cancer via a series of experiments. Then, we performed luciferase assay and RNA immunoprecipitation (RIP) assay to explore the competing endogenous RNA (ceRNA) function of circEPSTI1 in ovarian cancer. qRT-PCR verified that circEPSTI1 was overexpressed in ovarian cancer. Inhibition of circEPSTI1 suppressed ovarian cancer cell proliferation, invasion but promoted cell apoptosis. Luciferase assays and RIP assay showed that circEPSTI1 and EPSTI1 (epithelial stromal interaction 1) could directly bind to miR-942. And circEPSTI1 could regulate EPSTI1 expression via sponging miR-942. In summary, circEPSTI1 regulated EPSTI1 expression and ovarian cancer progression by sponging miR-942. circEPSTI1 could be used as a biomarker and therapeutic target in ovarian cancer.

11.
Plant Physiol Biochem ; 138: 36-47, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30844693

RESUMO

Brassinosteroids (BRs) regulate plant growth and stress responses. BRASSINOSTEROID-INSENSITIVE 1 (BRI1) is a BR receptor that perceives BRs and subsequently activates BR signaling. However, how BR contents and BRI1 expression levels affect the drought resistance of tomato requires further investigation. Here, we exogenously applied 24-epibrassinolide (EBR) and brassinazole (Brz) to tomato plants and generated different transgenic tomato SlBRI1 overexpression lines to study the drought stress response. Our results showed that EBR application 3 days before drought stress increased the contents of BRs and decreased abscisic acid (ABA) and reactive oxygen species (ROS), after which stomatal aperture and drought resistance eventually increased. Brz application reduced the drought resistance. Astonishingly, overexpression of 35S:SlBRI1, which increased BR signaling intensity, led to slightly improved contents of ABA and ROS and ultimately reduced both stomatal aperture and drought resistance. Moreover, plants expressing SlBRI1 driven by a stress-inducible promoter (Atrd29A) also exhibited reduced plant drought resistance. In all cases, enhancing the BR signaling intensity reduced antioxidant enzyme activity and reduced the expression of drought stress-related genes, ultimately compromising the drought resistance. Additionally, SlBRI1 mutants with altered brassinolide sensitivity (abs), which was weak BR signaling, exhibited significantly increased drought resistance. Therefore, our results reveal that BR contents positively regulated tomato drought resistance and that BR signaling intensity via BRI1 was negatively related to the drought resistance. These imply that the increased drought resistance in response to BRs is a newly discovered BR signaling branch that is located downstream of BRs and that differs from that of BRI1.


Assuntos
Brassinosteroides/metabolismo , Resistência à Doença , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum , Proteínas de Plantas/biossíntese , Proteínas Quinases/biossíntese , Transdução de Sinais , Desidratação , Lycopersicon esculentum/enzimologia , Lycopersicon esculentum/genética , Proteínas de Plantas/genética , Proteínas Quinases/genética
12.
Arch Pharm Res ; 42(4): 344-358, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28887616

RESUMO

Previous studies demonstrated that depression is more prevalent in women with polycystic ovary syndrome (PCOS). In this study, we aimed to determine whether amitriptyline (AMT), an antidepressant drug, plays a role in preventing PCOS. The results showed that AMT modified ovarian morphology improved the ovarian functions and estrus cycle in estradiol valerate (EV)-induced polycystic ovary (PCO). AMT restored the levels of estradiol (E2), testosterone (T) and progesterone (P4) to normal, and elevated the level of luteinizing hormone (LH) in EV-induced PCO. No significant changes in follicle stimulating hormone (FSH) levels were observed in rats with EV or AMT treatment. The restoration of norepinephrine (NE) level was detected in rats with EV-induced PCO. AMT also altered the expression levels of steroidogenesis genes and beta2-adrenoceptor (beta2-AR) in EV-induced PCO. Our data revealed that AMT improves the ovarian morphology and modifies ovarian expression of beta2-AR and steroidogenesis genes in rats with EV-induced rat PCO. Our data provide support for the hypothesis that AMT is considered as a candidate drug for preventing and treating PCOS along with depression.


Assuntos
Amitriptilina/farmacologia , Antidepressivos/farmacologia , Ovário/efeitos dos fármacos , Síndrome do Ovário Policístico/tratamento farmacológico , Animais , Estradiol , Feminino , Ovário/metabolismo , Ovário/patologia , Síndrome do Ovário Policístico/induzido quimicamente , Síndrome do Ovário Policístico/patologia , Ratos , Ratos Wistar , Receptores Adrenérgicos beta 2/genética , Receptores Adrenérgicos beta 2/metabolismo , Espermatogênese/efeitos dos fármacos , Espermatogênese/genética
13.
Sci Rep ; 8(1): 17960, 2018 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-30568250

RESUMO

The Na(3p) + H2(X1Σg+) → NaH(X1Σ+) + H(2S) reaction plays an important role in the field of diabatic reaction dynamics. A set of new diabatic potential energy surfaces (PESs) of the NaH2 system are structured, which include the diabatic coupling between the lowest two adiabatic states. The electronic structure calculations are performed on the multi-reference configuration interaction level with the cc-pwCVQZ and aug-cc-PVQZ basis sets for Na and H atoms. 32402 geometries are chosen to construct the diabatic data by a unitary transformation based on the molecular property method. The diabatic matrix elements of [Formula: see text], [Formula: see text] and [Formula: see text] ([Formula: see text]) are fitted by the artificial neural network model. The spectroscopic constants of diatoms obtained from the present PESs are consistent with the experimental data. The topographical and intersection characteristics of the [Formula: see text] and [Formula: see text] surfaces are discussed. Based on the new PESs, the time-dependent quantum wave packet calculations are carried out to study the reaction mechanism of the title reaction in detail.

14.
Cell Mol Biol Lett ; 23: 52, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30410547

RESUMO

Background: Pulmonary inflammation and endothelial barrier permeability increase in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) induced by pro-inflammatory cytokines and matrix metalloproteinases (MMPs). However, the relationship between pro-inflammatory cytokines and MMPs in ALI/ARDS remains poorly understood. Methods: A lipopolysaccharide (LPS)-induced ALI rat model was established through intratracheal instillation. The wet/dry ratios of lung tissues were measured, and bronchoalveolar lavage fluid (BALF) was collected to test protein concentrations, total cell/macrophage numbers, and pro-inflammatory cytokine levels. LPS-treated alveolar macrophages were utilized in in vitro experiments. The expression and secretion of MMPs were respectively detected using quantitative PCR, Western blotting and ELISA assays. Results: The levels of IL-33 and MMP2/9 in BALF increased in all the ALI rats with severe lung injury. LPS-induced IL-33 autocrine upregulated the expression of MMP2 and MMP9 through activating STAT3. Neutralizing IL-33 in culture medium with specific antibodies suppressed the expression and secretion of MMP2 and MMP9 in LPS-treated alveolar macrophages. Consistently, eliminating IL-33 decreased the levels of MMP2 and MMP9 in BALF and alleviated lung injury in ALI rats. Conclusion: The IL-33/STAT3/MMP2/9 regulatory pathway is activated in alveolar macrophages during acute lung injury, which may exacerbate the pulmonary inflammation.


Assuntos
Lesão Pulmonar Aguda/metabolismo , Interleucina-33/metabolismo , Macrófagos Alveolares/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Fator de Transcrição STAT3/metabolismo , Lesão Pulmonar Aguda/patologia , Animais , Células Cultivadas , Modelos Animais de Doenças , Técnicas de Silenciamento de Genes , Mediadores da Inflamação/metabolismo , Lipopolissacarídeos , Macrófagos Alveolares/patologia , Masculino , Testes de Neutralização , Ratos Wistar , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
15.
3 Biotech ; 8(7): 311, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30003000

RESUMO

High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small RNA libraries and one degradome library. A total of 518 known and 976 novel miRNAs were identified, of which, 338 known and 18 novel miRNAs were expressed in all six libraries, respectively. A total of 52 known and 57 novel miRNAs were identified to be significantly differentially expressed between RE and RL, and 195 mRNAs were verified to be the targets of 194 miRNAs by degradome sequencing. According to the degradome analysis, 11 differentially expressed miRNAs had miRNA-mRNA targets, and 13 targets were identified for these 11 miRNAs. Of the 13 miRNA-mRNA targets, 4 genes (RSG11079.t1, RSG11844.t1, RSG16775.t1, and RSG42419.t1) were involved in hormone-mediated signaling pathway, 2 gens (RSG11079.t1 and RSG16775.t1) were related to post-embryonic root development, and 1 gene (RSG23799.t1) was involved in anatomical structure morphogenesis, according to the GO function analysis for biological process. Target Genes participated in these processes are important candidates for further studies. This study provides valuable information for a better understanding of the molecular mechanisms involved in radish tuberous root formation and development.

16.
Phys Chem Chem Phys ; 20(31): 20641-20649, 2018 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-30059113

RESUMO

The study of K + H2 collision has a long experimental history, but there have been few theoretical studies due to lack of a global potential energy surface (PES). In this study, a new global PES for the ground state of KH2 system was constructed based on numerous ab initio points, using the permutation invariant polynomial neural network method. The root mean square error (RMSE) of PES is very small (5.64 meV). On the new PES, time-dependent quantum wave packet (TDWP) and quasiclassical trajectory (QCT) calculations were carried out to study the dynamics of the K(2S) + H2(X1Σ+g) → H(2S) + KH(X1Σ+) reaction. Dynamics results show that (i) the K + H2(v = 0) → H + KH reaction scarcely occurred, (ii) the K + H2(v = 1) → H + KH reaction took place in small quantities, and (iii) the K + H2(v = 2) → H + KH reaction occurred in large quantities. This indicates that vibrational energy of the reactant is significantly more effective at promoting the reaction than the translational energy. This characteristic stems from a major physical model in reactive collisions: the vibrationally excited H2 molecule and K atom collide first in a T-geometric configuration and the vibrational motion of the H2 molecule helps separate the two H atoms a large distance after the collision. At a large H-H distance, a broad well exists on the PES, so the heavy K atom could pull back the light H atom to initiate the reaction. Similar to the reactive channel, vibrational excitation of the reactant also has a significant effect on the collision-induced dissociation channel.

17.
Int J Oncol ; 53(1): 286-296, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29749456

RESUMO

The incidence of intrahepatic cholangiocarcinoma (ICC) is progressively increasing worldwide, and its prognosis remains poor. Accumulating evidence has demonstrated that tumor necrosis factor receptor-associated factor 4 (TRAF4), an adaptor protein, is involved in the carcinogenesis and progression of several tumor types. However, the function of TRAF4 in predicting prognosis, and mediating migration and invasion of ICC remains to be elucidated. In the present study, immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction assays were used to determine that the expression of TRAF4 at the mRNA and protein levels in ICC tissues was significantly higher compared with that in non­tumor tissues. The overexpression of TRAF4 was positively correlated with poor differentiation, regional lymphatic metastasis, and high tumor­node-metastasis staging. Inhibiting the expression of TRAF4 using small interfering RNA decreased the migration and invasion of ICC cells in vitro. In addition, the AKT inhibitor perifosine eliminated the effect of TRAF4 on the invasion and migration of ICC cells in vitro. Clinically, the overexpression of TRAF4 was correlated with shorter overall survival rate and elevated recurrence rate in patients with ICC. Furthermore, patients with ICC with a high expression of TRAF4 and lymphatic metastasis were closely associated with a poorer prognosis compared with the other groups. Multivariate analysis indicated that the overexpression of TRAF4 was an independent prognostic indicator for patients with ICC. It was identified that a high level of TRAF4 facilitated the invasiveness of ICC cells via the activation of AKT signaling. The overexpression of TRAF4 may be a prognostic biomarker and candidate therapeutic target for patients with ICC.


Assuntos
Colangiocarcinoma/genética , Invasividade Neoplásica/genética , Prognóstico , Fator 4 Associado a Receptor de TNF/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Colangiocarcinoma/patologia , Intervalo Livre de Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/patologia
19.
J Environ Manage ; 213: 247-254, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29502010

RESUMO

Discharge of urban domestic pollution has risen sharply during China's extensive urbanization. Together with understanding the complexity of influencing factors underpinning this rise, it has become a pressing issue to estimate total discharge and illustrate its driving mechanism scientifically. This paper reports on the monitoring of discharge from 36 sampling sites in selected residential districts in the heavily polluted Taihu Basin, China. The data were used to estimate the total amount of discharge, to develop corresponding urban domestic pollutant discharge coefficients and to analyse associated spatial patterns. Data from a questionnaire survey of over 1000 households in downtown, suburb and market town areas were then used to apply an econometric model in order to distinguish driving mechanisms. The urban domestic pollutant discharge coefficients developed in this paper are generally smaller than those reported nationally for China, based on more generalised data, decaying from city centres to the urban periphery. This study quantifies the amount of discharge and also demonstrates that urban domestic pollutant discharge is driven by multiple factors. For example, urban domestic pollution discharge rates were positively correlated with income and female-dominated households also tend to discharge more wastewater. Other factors were found to have negative correlations, such as sewage treatment rates, awareness of environmental protection, age and degree of education. As well as providing new and refined data on urban pollution discharge characteristics, the research in this paper also demonstrates the utility of combining household questionnaire and sample monitoring data in order to yield greater insights into the causes of typical polluting behaviour in Chinese neighbourhoods.


Assuntos
Monitoramento Ambiental , Urbanização , Gerenciamento de Resíduos , China , Cidades , Feminino , Humanos , Características de Residência
20.
Phys Chem Chem Phys ; 20(9): 6638-6647, 2018 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-29457183

RESUMO

The global diabatic potential energy surfaces (PESs) of the MgH2+ system, which can be used to study the dynamics of the Mg+(3p2P) + H2(X1Σ) → MgH+(X1Σ+) + H(2S) reaction, are structured for the first time. The diabatic PESs are correlated with the ground state 12A' and the first excited state 22A'. The multi-reference configuration interaction method and the VQZ basis set are used in ab initio calculations, and diabatic potential energies are calculated based on the molecular properties of the dipole moment. The neural network method is applied to fit the matrix elements of the diabatic energy surfaces. Spectroscopic constants of H2(X1Σg+) and MgH+(X1Σ+) obtained from the new PESs agree well with the experimental data. Based on the diabatic PESs of MgH2+, the time-dependent wavepacket calculations for the Mg+(3p2P) + H2(X1Σg+) → MgH+(X1Σ+) + H(2S) reaction are carried out to study the reaction dynamics. There is no threshold for this reaction because of the existence of barrierless reactive paths. The reaction has a high total integral cross section (ICS), and vibrationally resolved ICSs show an obvious population inversion of product vibrational states. The results of differential cross sections (DCSs) indicate that most product molecules tend to forward scatter.

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