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1.
PLoS One ; 15(1): e0228159, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32004347

RESUMO

Proteolytic processing of Bacillus thuringiensis (Bt) Cry protoxins by insect midgut proteases is critical to their insecticidal activities against target insects. Although transgenic Bt cotton expressing Cry1Ac and Cry2Ab proteins have been widely used for control of the cotton bollworm (Helicoverpa armigera) in the field, the proteolytic cleavage sites in the two protoxins targeted by H. armigera midgut proteases are still not clear. In this study, the proteolysis of Cry1Ac and Cry2Ab protoxins by midgut juice prepared from midgut tissue of H. armigera larvae was investigated. Cleavage of Cry1Ac protoxin by midgut proteases formed a major protein fragment of ~65 kDa, and N-terminal sequencing revealed that cleavage occurred at Arg28 in the fore-end of helix α-1 in domain I of Cry1Ac. Cleavage of Cry2Ab protoxin by midgut juice proteases produced a major protein fragment of ~50 kDa, and the cleavage occurred at Arg139 between helices α-3 and α-4 in domain I of Cry2Ab. The amino acids Arg28 of Cry1Ac and Arg139 of Cry2Ab were predicted as putative trypsin cleavage sites. Bioassay data showed that the toxicities (LC50s) of Cry1Ac and Cry2Ab protoxins were equivalent to those of their respective midgut juice-activated toxins in the susceptible SCD strain of H. armigera. Identification of the exact sites of N-terminal activation of Cry1Ac and Cry2Ab protoxins will provide a basis for a better understanding of the mode of action and resistance mechanisms based on aberrant activation of these protoxins in H. armigera.

2.
Expert Opin Biol Ther ; : 1-7, 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32033522

RESUMO

Introduction: Immunotherapy is now a standard treatment for many malignancies. Although immune checkpoint inhibition has demonstrated substantial efficacy by enhancing T cell activation and function in the tumor microenvironment, adoptive transfer of T and NK cell products promises to provide activated cells capable of immediate and direct tumor destruction. A widely applicable, non-MHC dependent, cellular therapy, consisting of in vitro generated dendritic cells (DC) combined with cytokine-induced killer cells (CIK), is highly efficient to produce from individual patients and has demonstrated safety and efficacy alone or with chemotherapy.Areas covered: We summarize the clinical data from studies of DC-CIK and discuss future research directions.Expert opinion: Patients with a wide variety of tumor types who have received DC-CIK therapy may experience clinical responses. This versatile therapy synergizes with other anti-cancer therapies including chemotherapy and immunotherapy.

3.
Sci Total Environ ; 710: 136306, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32050365

RESUMO

Acetic acid and sodium acetate are generally supplied to wastewater treatment plants (WWTPs) in China to improve total nitrogen (TN) and total phosphorus (TP) removal, and the addition of carbon source also facilitates to increase sludge growth rate and further provides material basis for the extraction of proteins and amino acids from activated sludge. To recycle ammonia nitrogen resources, a system that combined adsorption and anaerobic-anoxic-oxic (A/AAO) process for treating low strength wastewater was established. Experimental results showed that by the addition of carbon substrate from a mixture of anaerobically fermented adsorption sludge, the average removal efficiency of chemical oxygen demand (COD), ammonia nitrogen, TN, and TP were 88%, 96.9%, 93.9%, and 92.1%, respectively, and the ratio of nitrogen assimilation to nitrogen dissimilation significantly increased by a factor of 2.5. Through energy analysis (based on adenosine triphosphate, ATP), sludge flocculation capacity and settling property, it was found that the AAO process sludge presented the logarithmic growth characteristics. The respective sludge protein and amino acids contents increased by over 11.4% and 40.3%, and the synthetic products of glutamic acid, alanine and aspartate increased through the assimilation of ammonia nitrogen, thereby indicating that replenishing the carbon substrate could markedly enhance protein and amino acids contents in AAO process sludge. Moreover, the diversity of the microbial community in adsorption process was relatively rich, the diversity in the adsorption process sludge was the highest, while the diversity of the AAO process sludge evidently decreased. The microbial community in each process was similarly based on 16S rDNA gene sequence analysis, microflora was prominent in the AAO process, with Dechloromonas, Flavobacterium, Zoogloea, Unclassified_Rhodocyclaceae and Thauera as the dominant species. Promising carbon utilization facilitates contaminants removal in low strength wastewater treatment and is conducive to protein production through ammonia nitrogen assimilation.

5.
Food Chem Toxicol ; 137: 111167, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32007468

RESUMO

Chronic exposure to n-hexane could induced serious peripheral nerve impairments. It has been well documented that the metabolic activation from n-hexane to 2,5-hexanedione (2,5-HD) is vital in the pathogenesis. Diallyl sulfide (DAS) is an extract of garlic and able to block the bioactivation of xenobiotic. The current study was designed to investigate whether DAS can attenuate n-hexane induced neuropathy. Male Wistar rats were pretreated with DAS (50 or 100 mg/kg.bw) and then n-hexane (3 g/kg.bw) for 7 weeks. Behavioral performance, biomarker measurement and toxicokinetic studies were performed. Enzymatic methods and western blotting analyses were also conducted to investigate the hepatic phase I enzymes (including cytochrome P450(CYP)2E1, CYP1A1 and CYP2B1) and phase II enzymes (including glutathione S transferase theta 1 (GSTT1) and NA(D)PH dehydrogenase quinone 1 (NQO1)). The results showed that DAS improved the behavioral performance while reducing the toxic metabolite: 2,5-HD and pyrrole adducts. Besides, DAS reduced the expression of CYP2E1 with a proportional decrease in activity, which largely decreased the bioactivation of n-hexane in vivo. The results suggested that DAS decreased the toxic metabolites of n-hexane to attenuate n-hexane-induced peripheral neuropathy.

6.
ACS Chem Neurosci ; 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32023408

RESUMO

Conformational transition from the normal cellular form of prion protein (PrPC) to pathogenic "Scrapie" form (PrPSc) is considered to be a key event to the occurrence of prion disease. Additionally, the H2 C-terminus is extensively considered to be a vital site for PrP conformational transition, which can be used as an important region to explore the potential mechanism of PrP misfolding. Therefore, to study the misfolding mechanism of PrP, 500 ns well-tempered metadynamics simulations were performed by focusing on the H2 C-terminus of PrP. For comparison, three systems were designed in total, including PrP in neutral and acidic conditions as well as H187R mutant. The resulting free energy surfaces (FESs) obtained from metadynamics simulations reveal that acidic condition and H187R mutation can facilitate PrP misfolding by decreasing free energy barriers for conformational transition and forming energy stable conformational states. Further analyses aiming at H2 C-terminus show that due to the increase of positive charge of residue 187 in both acidic and H187R systems, the electrostatic repulsion of residue 187 and R136/R156 increases largely, which disrupts the electrostatic interaction network around H2 C-terminus and makes the hydrophobic core exposed to the solvent. Taken together, the acidic condition and H187R mutation can accelerate PrP misfolding mainly by forming more energy stable metastable conformations with lower free energy barriers, and electrostatic network disruption involving residue 187 drives the initial misfolding of H2 C-terminus. This study provides a quantitative insight into the related function of the H2 C-terminus in the PrP misfolding process, which may guide the H2 C-terminus mediated drug design in the future.

7.
Mol Cell Endocrinol ; : 110756, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32045626

RESUMO

Exosomes have been intensively studied in autoimmune diseases, and circulating exosomes and microvesicles have also been explored in autoimmune thyroiditis (AITD). However, the role of thyroid cell-derived exosomes in immune responses is unclear. We showed that IFN-γ-treated Nthy-ori 3-1 cell-derived exosomes (IFN-γ-Exo) harbored TPO, HSP60 and MHC-II and activated dendritic cells (DCs) in vitro. Compared with Exo-targeted DCs (DCExo), IFN-γ-Exo-targeted DCs (DCIFN-γ-Exo) promoted the expression and release of proinflammatory cytokines, such as IFN-γ, IL-17A and IL-22, from CD4+ T lymphocytes and inhibited the expression and release of anti-inflammatory cytokines, such as IL-4, IL-10 and TGF-ß1; however, IFN-γ-Exo did not have this effect compared with Nthy-ori 3-1 cell-derived exosomes (Exo). DCIFN-γ-Exo stimulates the expression and release of cytokines from CD4+ T lymphocytes more efficiently than IFN-γ-Exo. Thus, DCIFN-γ-Exo may effectively induce CD4+ T lymphocyte-mediated immune responses and play a role in the occurrence and development of AITD.

8.
Anal Chem ; 92(3): 2672-2679, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31898456

RESUMO

Sulfur-containing species (SCS), especially sulfur dioxide-relevant species, play an essential role in ecological balance. Owing to the intrinsically labile and mobile characteristics of SCS, it is still considered to be an insurmountable challenge for multiplexed tracking dynamics of SCS with distinct molecular structure, valence state, and condensed state. To address this key problem, we proposed herein alternative versatile single-molecule sensors (VSMs) that intramolecularly integrate high affinity target-guided multiple recognition units into a single sensory molecule, clarified as molecular Nezha available in triplexed responses to gaseous sulfur dioxide, liquid sulfur trioxide, and aqueous bisulfite through ubiquitous charge transfer and nucleophilic addition. High-performance molecular Nezha remarkably facilitated promising applications in a quantitative visualization of SCS on lab-on-paper and tracking the dynamics transformation of SCS as well comprehensive evaluation of multiphase adsorption science of SCS on an advanced Zeolitic imidazolate framework-8 (ZIF-8).

9.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 118003, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31923786

RESUMO

Biological thiols including homocysteine (Hcy), cysteine (Cys), hydrogen sulfide (H2S) and glutathione (GSH) play crucial roles in various pathological and physiological processes. The development of optical probes for biothiols has been an active research area in recent years. Herein, a new turn-on fluorescence probe (HD-NBD) was designed and synthesized by fusing tetrahydro[5]helicene and 7-nitro-2,1,3-benzoxadiazole (NBD) for simultaneous discrimination of Hcy/Cys, H2S and GSH in aqueous solution. This probe is able to show unique absorbance enhancement at 548 nm for H2S and additional fluorescence enhancement at 536 nm only for Cys/Hcy, which can be used to discriminate H2S, Cys/Hcy and GSH simultaneously. In addition, HD-NBD also shows low background without any self-fluorescence, as well as high selectivity toward common biothiols. The low detection limits of this probe are about 0.15 µM for Hcy with a wide linear range (1-80 µM), 0.36 µM for Cys (linear range: 1-45 µM), 0.79 µM for H2S (linear range: 1-80 µM) and 4.44 µM for GSH (linear range: 1-60 µM). Moreover, HD-NBD can identify Hcy/Cys, H2S from GSH and other amino acids with high sensitivity and selectivity, therefore it could be used for detecting endogenous and exogenous Hcy/Cys under biological condition.

10.
Nanoscale ; 12(4): 2686-2694, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31916567

RESUMO

Carbon nanocones (CNCs) are promising drug delivery systems that can be functionalized with a variety of biomolecules (such as proteins, peptides, or antibodies), which allow for site-specific, targeted payload delivery to particular cells and organs. However, considerable uncertainty exists with respect to the toxicity of CNCs on their conical shape, and the underlying mechanism that leads to the penetration of CNCs (especially the truncated ones) in and through the cell membrane is not yet well understood. Using a coarse-grained dissipative particle dynamics method, we systematically investigate the spontaneous penetration of untruncated and truncated CNCs into membrane vesicles. For untruncated CNCs, the simulation results show that both pristine and oxidized ones can spontaneously penetrate across or be attached to the vesicle surface without membrane rupture, indicating low or insignificant toxicity. However, for truncated CNCs, we find that both the apex angle and aspect ratio can influence the CNC-membrane interactions and CNC-induced toxicity: a higher apex angle (and/or a lower aspect ratio) yields a higher toxicity of truncated CNCs. Further free energy analysis reveals that the lowest free energy path during the penetration is associated with CNC's orientation and rotation. For a truncated CNC with a low aspect ratio and high apex angle, it tends to rotate itself to a preferred standing-up fashion inside the vesicle membrane, posing an enhanced toxicity of CNCs. These findings may provide useful guidelines for designing effective CNC vehicles for drug delivery.

11.
J Hazard Mater ; 387: 122011, 2020 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-31927354

RESUMO

Nowadays, designing highly active photocatalysts for pollutant degradation under visible light still remains a challenging problem. Herein, a novel benzothiadiazole functionalized Co-doped MOF-based photocatalyst with electron deficient unit was first synthesized via a feasible step-by-step assembly strategy. Benzothiadiazole, as typical electron deficient group, could effectively promote the separation and transfer of photoinduced charge carriers. The implantation of Co ion could be served as an effective mediator to further facilitate the charge transfer through a Co3+/Co2+ redox pathway. Interestingly, the as-synthesized Co-MIL-53-NH-BT exhibited significantly enhanced photocatalytic degradation capacity for BPA and OFX under visible light irradiation, with removal efficiency as high as 99.9 % and 99.8 % within 120 min. TOC analysis suggested that majority of contaminants had been degraded into CO2 and H2O. The important parameters influencing the photocatalytic activity were investigated, and the kinetics study was also conducted. The possible degradation pathways and the possible photocatalytic mechanism were proposed. More importantly, the as-synthesized Co-MIL-53-NH-BT showed good reusability, stability as well as universal applicability. To sum up, current work not only developed an efficient and visible-light active photocatalyst for treating organic-contaminated wastewater, but also afforded some novel insight into the utilization of benzothiadiazole in MOF-based photocatalyst towards improving photocatalytic activity.

12.
Bioresour Technol ; 302: 122825, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31986335

RESUMO

The removal of antibiotics, antibiotic-resistant bacteria (ARB), and cell-free antibiotic-resistant genes (ARGs) and the microbial community of ARB were investigated in detail to understand their fate and provide valuable information on the feasibility of full-scale membrane bioreactor (MBR). The potential risks of cell-free ARGs to the receiving environment were discovered. High influent antibiotic concentration could inhibit the microbial activity of MBR sludge, whereas good antibiotic removal could be maintained because of relatively long solid retention time and high biomass retention. Approximately 61.8%-77.5% of the total antibiotics were degraded, and 22.5%-38.2% of the total antibiotics were adsorbed by MBR sludge on average. The individual antibiotic removal presented intense discrepancy because of the chemical construction and distribution coefficient of antibiotics. Aeromonas exhibited specific antibiotic resistance to ampicillin and erythromycin, Escherichia became the predominant genera in kanamycin-ARB and tetracycline-ARB, and Klebsiella and Bacteroides were the particular genera that exhibited distinct antibiotic resistance to ciprofloxacin. A significant correlation was found between cell-free ARG abundance and ARB content, and relatively high effluent cell-free ARG abundance facilitated the proliferation and transmission of ARB. The impacts of the receiving environment to eliminate the ecological risks and severe threats to human health should be investigated because of the low decay ratio and long-term persistence of cell-free ARGs.

13.
Anal Bioanal Chem ; 412(3): 611-620, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31900539

RESUMO

Infections caused by foodborne microorganisms are a great threat to the global environment and public healthcare today. Thus, rapid, portable and sensitive assays that can realize the identification of foodborne bacteria are highly desired. In this study, a smart fluorescent and colorimetric dual-readout sensing system has been established for simple and rapid E. coli determination by utilizing the Cu2+-triggered oxidation of o-phenylenediamine (OPD). Initially, Cu2+ can oxidize OPD to OPDox, resulting in an orange-yellow fluorescence and visible pale-yellow color. However, E. coli can effectively reduce Cu2+ into Cu+, inhibiting the Cu2+-triggered oxidation of OPD to OPDox. Consequently, the introduction of E. coli can turn off both the fluorescence and the UV-vis absorbance signals of the OPD-Cu2+ system, illustrating an original mechanism for fluorescent and colorimetric dual-channel detection of E. coli. Moreover, a filter paper-based visual sensor was built and coupled with OPD-Cu2+ solution under the assistance of a UV lamp. The as-prepared sensor can detect E. coli quantitatively with the help of a typical smartphone color-scanning application (APP). Thus, this study offers a valid dual-mode assay for sensitive and on-site visible detection of E. coli, guaranteeing the reliability of the results and is more attractive for practical use. Graphical Abstract Schematic illustration of the smartphone-integrated sensing system for fluorescent and colorimetric dual-channel detection of E. coli based on the Cu2+-OPD system.


Assuntos
Bactérias/isolamento & purificação , Colorimetria/métodos , Corantes Fluorescentes/química , Microbiologia de Alimentos , Papel , Smartphone , Espectrometria de Fluorescência/métodos , Integração de Sistemas , Bactérias/patogenicidade , Técnicas Biossensoriais , Reprodutibilidade dos Testes , Espectrofotometria Ultravioleta/métodos
14.
J Hazard Mater ; 389: 122074, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31978818

RESUMO

Extensive use of organophosphorus pesticides (OPs) in crop protection has aroused worldwidely great concern about safety and the detection of OPs is of great significance to food safety and human health. In this work, peptides attached with tetraphenylethylene (TPE) molecule were synthesized to from an aggregation-induced emission fluorescent probe (TPE-Peptide) for the determination of OPs. The working mechanism was as follows: in presence of OPs, OPs would react with active site serine in the peptide sequence via covalent bond and adducts were formed between OPs and the peptides; once formed, the adducts accelerated the aggregation of peptides, thus inducing strong emission of TPE-Peptide probe. So the adducts formation and the enhanced emission of the TPE-Peptide probe were the key factors for the OPs' sensing. Herein, the adducts formed between OPs and TPE-Peptide probe, the aggregated peptide fibrils were characterized by fluorescence, mass spectrometry, transmission electron microscopy, dynamic light scattering, atomic force microscopy, circular dichroism spectra and confocal fluorescence microscopy etc. This TPE-Peptide probe displayed highly sensitive fluorescence response where OPs' concentrations ranged from 1 to 100 µM with the limit of detection 0.6 µM and also showed selectivity.

15.
Anal Bioanal Chem ; 412(6): 1343-1351, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31901961

RESUMO

In this study, a novel fluorescent "turn-on" aptasensor was developed for sensitive and rapid detection of tetracycline (TC) in animal-derived food. It is based on aptamer-functionalized nitrogen-doped graphene quantum dots (N-GQDs-aptamer) coupled with cobalt oxyhydroxide (CoOOH) nanoflakes. The CoOOH nanoflakes are efficient fluorescence quenchers in homogeneous solutions, and this is due to their advantages of excellent optical properties, superior flexibility, and water dispersibility. The proposed method's mechanism is driven by quenching based on the fluorescence resonance energy transfer (FRET) between the donor (N-GQDs) and the acceptor (CoOOH nanoflakes). On the other hand, fluorescence recovery is caused by the structure switching behavior of the aptamer. Compared with previous methods, our developed method exhibits better behavior in terms of being easy to fabricate and being simple in detection procedure and maintains the detection limit low enough in TC determination: a linear range from 1 to 100 ng mL-1 and a detection limit of 0.95 ng mL-1 (S/N = 3). Furthermore, the proposed method was applied to five animal-derived food samples (milk, honey, fish, eggs, and chicken muscle) and demonstrated practical applicability. As well, the method has the advantages of simplicity in pre-treatment and convenience in instruments, saves times, and is cost-effective. Finally, the proposed method demonstrates significant potential for sensitive and rapid detection of specific components in real samples. Graphical abstract.

16.
Sensors (Basel) ; 20(2)2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31936007

RESUMO

Although graphene has been widely used as a nano-filler to enhance the conductivity of porous materials, it is still an unsatisfactory requirement to prepare graphene-based sponge porous materials by simple and low-cost methods to enhance their mechanical properties and make them have good sensing and capacitive properties. Graphene platelets (GnPs) were prepared by the thermal expansion method. Graphene-based sponge porous materials were prepared by a simple method. A flexible sensor was formed and supercapacitors were assembled. Compared with other graphene-based composites, the graphene-based composite sponge has good electrical response under bending and torsion loading. Under 180° bending and torsion loading, the maximum resistance change rate can reach 13.9% and 52.5%, respectively. The linearity under tension is 0.01. The mechanical properties and capacitance properties of the sponge nanocomposites were optimized when the filler fraction was 1.43 wt.%. The tensile strength was 0.236 MPa and capacitance was 21.4 F/g. In cycles, the capacitance retention rate is 94.45%. The experimental results show that the graphene-based sponge porous material can be used as a multifunctional flexible sensor and supercapacitor, and it is a promising and multifunctional porous nanocomposite material.

17.
Genes (Basel) ; 11(1)2020 01 08.
Artigo em Inglês | MEDLINE | ID: mdl-31936280

RESUMO

Lactobacillus ruminis is a commensal motile lactic acid bacterium living in the intestinal tract of humans and animals. Although a few genomes of L. ruminis were published, most of them were animal derived. To explore the genetic diversity and potential niche-specific adaptation changes of L. ruminis, in the current work, draft genomes of 81 L. ruminis strains isolated from human, bovine, piglet, and other animals were sequenced, and comparative genomic analysis was performed. The genome size and GC content of L. ruminis on average were 2.16 Mb and 43.65%, respectively. Both the origin and the sampling distance of these strains had a great influence on the phylogenetic relationship. For carbohydrate utilization, the human-derived L. ruminis strains had a higher consistency in the utilization of carbon source compared to the animal-derived strains. L. ruminis mainly increased the competitiveness of niches by producing class II bacteriocins. The type of clustered regularly interspaced short palindromic repeats /CRISPR-associated (CRISPR/Cas) system presented in L. ruminis was mainly subtype IIA. The diversity of CRISPR/Cas locus depended on the high denaturation of spacer number and sequence, although cas1 protein was relatively conservative. The genetic differences in those newly sequenced L. ruminis strains highlighted the gene gains and losses attributed to niche adaptations.

18.
Nat Plants ; 6(1): 34-45, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31932676

RESUMO

Rapeseed (Brassica napus) is the second most important oilseed crop in the world but the genetic diversity underlying its massive phenotypic variations remains largely unexplored. Here, we report the sequencing, de novo assembly and annotation of eight B. napus accessions. Using pan-genome comparative analysis, millions of small variations and 77.2-149.6 megabase presence and absence variations (PAVs) were identified. More than 9.4% of the genes contained large-effect mutations or structural variations. PAV-based genome-wide association study (PAV-GWAS) directly identified causal structural variations for silique length, seed weight and flowering time in a nested association mapping population with ZS11 (reference line) as the donor, which were not detected by single-nucleotide polymorphisms-based GWAS (SNP-GWAS), demonstrating that PAV-GWAS was complementary to SNP-GWAS in identifying associations to traits. Further analysis showed that PAVs in three FLOWERING LOCUS C genes were closely related to flowering time and ecotype differentiation. This study provides resources to support a better understanding of the genome architecture and acceleration of the genetic improvement of B. napus.

19.
Food Chem ; 312: 126083, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31901823

RESUMO

Creatinine, commonly found in muscle tissue, has been demonstrated as an essential precursor of 2-amino-1-methyl-6-phenylimidazo [4, 5-b] pyridine (PhIP) in thermally possessed foods. In this current study, formamide and N-methylformamide were identified as the main thermal degradation products of creatinine. The raised production of PhIP and the decreased level of aldol condensation product occurred simultaneously with the increased addition of formamide in the model system of creatinine and phenylacetaldehyde. It was demonstrated that formamide reacted with the aldol condensation product to close the pyridine ring to form PhIP. The radical pathway of PhIP formation was then proposed and verified by density functional theory (DFT) computation and an ion-trap time-of-flight mass spectrometer (IT-TOF-MS). This is the first report of the role of the creatinine thermal degradation product in the pathway of PhIP formation, which could facilitate potential strategies against the inhibition of PhIP formation in thermally processed foods.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31916899

RESUMO

Background: Surgical management of complicated retroperitoneal mass is one of the most challenging urologic oncologic surgeries. This study aims to describe our technique and experience in dealing with retroperitoneal mass. Methods: Three patients with complicated retroperitoneal mass were treated with robot-assisted surgery with four arms through retroperitoneal approach. Surgical Procedure: Our standardized anatomic-based "kidney safe first, then mass resection" technique for robot-assisted complicated retroperitoneal mass resection focused on minimizing the chance of renal pedicle injury. Baseline demographics, pathology data, and latest follow-up outcome were obtained. Results: In this retrospectively reviewed case series, all 3 patients were successfully treated with robot-assisted surgery with four arms during retroperitoneal space. One patient received paravertebral mass resection 2 weeks after the robotic surgery. Mean data included operative time of 175 minutes, estimated blood loss was 133 mL, and hospital stay was 4 days. No complications occurred. Conclusions: Robot-assist surgery for complicated retroperitoneal mass with four arms is a safe and feasible way. Patient Summary: Mini-invasive treatment for retroperitoneal mass with robotic four arms through retroperitoneal approach is a feasible way. The approach reduces interruption of intracorporeal structure and organs. And patients could benefit from the retroperitoneal approach with a quicker recovery.

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