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1.
Chem Commun (Camb) ; 2021 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-33651061

RESUMO

A copper-catalyzed borylaminomethylation of multiple carbon-carbon bonds with N,O-acetal and bis(pinacolato)diboron has been disclosed that offers efficient and expedient access to γ-amino boronates. The products contain a valuable amine and boronate, which are amenable to further elaboration, and have versatile synthetic utilities.

3.
Wei Sheng Yan Jiu ; 50(1): 51-56, 2021 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-33517961

RESUMO

OBJECTIVE: To examine the vitamin B_(12) status of lactating women in China and to analyze the factors affecting the level of serum vitamin B_(12) in these subjects. METHODS: Participants were from the Chinese National Nutrition and Health Surveillance in 2013. By multi-stage stratified cluster randomly sampling method, 10 331 lactating women were selected from 55 counties of 30 provinces in China. Questionnaire was conducted to collect the basic information of lactating women and the dietary intake of them in the past one month was collected by food frequency questionnaire. A total of 20% of the total number of lactating women(a total of 1976) were randomly selected to analyze serum vitamin B_(12) by electro-chemiluminescence immunoassay method. Serum vitamin B_(12) deficiency was defined as a serum vitamin B_(12) level<200 pg/mL. The distribution of serum vitamin B_(12 )was lognormal, a generalized linear model was used to analyze the factors affecting the serum vitamin B_(12) level after logarithm transformation of it. RESULTS: The median of serum vitamin B_(12)(P25, P75) in lactating women was 469. 0(349. 0, 633. 5)pg/mL, the prevalence of vitaminB_(12) deficiency was 2. 7%(53/1976)and marginal deficiency rate was 12. 8%. With the increase of the education level of the lactating women the rate of vitamin B_(12) deficiency decreased gradually. The rate of vitamin B_(12 )deficiency of the lactating women presented regional differences and varied significantly among different regionals, education levels and the mothers who were breastfeeding or not. The value of serum vitamin B_(12 )was analyzed by generalized linear regression model showed that the levels of serum vitamin B_(12) in general rural counties and medium-sized or small cities were 1. 07 and 1. 13 times higher than those in poor rural areas, respectively. The serum vitamin B_(12) level of non-lactating women was 1. 08 times higher than that of lactating women. The serum vitamin B_(12 )level of the lactating women with junior high school level or below was significantly lower than that of the lactating women with college education and university education level, 88. 3% and 85. 0% of the lactating women with college education and university education, respectively. Serum vitamin B_(12) levels were positively correlated with intake of livestock meat, fish and seafood. CONCLUSION: The status of vitamin B_(12) in lactating women in China is good. Our findings suggest that some regionals, education levels, breastfeeding or not and livestock meat, fish meat and seafood supplement may be associated with serum vitamin B_(12 )in lactating women.


Assuntos
Lactação , Estado Nutricional , China/epidemiologia , Feminino , Humanos , Vitamina B 12 , Vitaminas
4.
Wei Sheng Yan Jiu ; 50(1): 57-62, 2021 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-33517963

RESUMO

OBJECTIVE: To investigate the relationship between household drinking water, toilet status and the prevalence of stunting for Chinese children aged 0 to 5 years old in 2013. METHODS: Data was extracted from the Chinese National Nutrition and Health Surveillance in 2013. A total of 32 861 children aged 0 to 5 years old were selected from 165 townships(streets) of 55 counties(cities/districts) in 30 provinces. Caregivers were face to face interviewed to collect data, including demographic characteristics of children, mothers and household, main source of household drinking water and main type of household toilet. Height/Length of children was measured using a standard procedure. Height(Length) for age Z score(HAZ(LAZ)) was calculated using WHO Anthro Survey Analyser software(online version), and stunting was defined as HAZ(LAZ)<-2. SAS 9. 4 was used to analyze. SURVEYFREQ procedure was used to calculate the coverage rate of safe drinking water, hygienic toilet, and the prevalence of stunting. SURVEYLOGISTIC procedure was used to analyze the relationship between household safe drinking water, hygienic toilet and prevalence of stunting for Chinese children aged 0 to 5 years old in 2013. RESULTS: A total of 32 861 children completed the study. The coverage rate of safe drinking water was 86. 9%. The proportion of water piped into household, a public tab and water from a protected spring or well was 49. 4%, 15. 9% and 15. 6%, respectively. The coverage rate of hygienic toilet was 56. 8%. The proportion of pour-flush toilet and pit latrine were 52. 1% and 4. 7%. The prevalence of stunting for 0 to 5 years old children was 8. 1% in China. The prevalence of stunting were 8. 1% for children aged 0-5 years old with safe household drinking water and 7. 8% for those without safe household drinking water. The difference was not significant(P=0. 882). The prevalence of stunting was 6. 6% for children aged 0-5 years old with hygienic household toilet and 10. 0% for those without hygienic household toilet. The difference was not significant(P=0. 069). There was no significant relationship between household sanitary condition and stunting for children aged 0 to 5 years old. CONCLUSION: Household sanitary conditions, including main source of household drinking water and the main type of household toilet may not be associated with the prevalence of stunting for children aged 0 to 5 years old in this settings.


Assuntos
Aparelho Sanitário , Água Potável , Criança , Pré-Escolar , China/epidemiologia , Feminino , Transtornos do Crescimento/epidemiologia , Humanos , Lactente , Recém-Nascido , Prevalência
5.
BMJ Open ; 10(12): e038362, 2020 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-33293388

RESUMO

INTRODUCTION: Two-thirds of women with symptoms of angina have 'angina with no obstructive coronary artery disease' (ANOCA). Growing evidence supports the use of coronary artery function testing for the diagnosis of ANOCA. Research into the prevalence of mental stress-induced myocardial ischaemia (MSIMI) among women with ANOCA is lacking. MSIMI is common in clinically stable patients with coronary artery disease. It is not associated coronary stenosis but is a prognostic risk factor. Here, we describe the rationale and protocol for a mechanistic clinical trial to test the following hypotheses: (1) that MSIMI is more common in women with ANOCA women than in age-matched and sex-matched controls, and (2) MSIMI is associated with mental stress-induced myocardial blood flow (MBF) change but not with adenosine vasodilator stress-induced MBF change. METHODS AND ANALYSIS: This is a mechanistic clinical trial. 84 women with confirmed ANOCA and 42 aged-matched healthy women (neither angina symptoms nor coronary stenosis) are to be recruited for mental and adenosine vasodilator stress tests. Positron emission tomography CT with ammonia N-13 will be used to evaluate the myocardial perfusion and MBF changes between stress and rest. MSIMI is defined as a summed difference score (SDS) of ≥3 and adenosine stress-induced myocardial ischaemia is defined as an SDS of ≥4. Other assessments include Reactive Hyperemia Index for microvascular endothelial function, peripheral arterial tonometry or digital vasomotor response, and a series of blood and psychometric tests. ETHICS AND DISSEMINATION: This mechanistic clinical trial was approved by the Ethics Committee of Guangdong Provincial People's Hospital. Findings will be disseminated through peer-reviewed publications and conference presentations. TRIAL REGISTRATION NUMBER: NCT03982901; Pre-results.

6.
Zootaxa ; 4881(2): zootaxa.4881.2.3, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33311314

RESUMO

Twenty-one new species of the genus Meleonoma Meyrick are described: M. acutata sp. nov., M. annulignatha sp. nov., M. artivalva sp. nov., M. basiprocessa sp. nov., M. bicornea sp. nov., M. bidigitata sp. nov., M. circinans sp. nov., M. compacta sp. nov., M. cuneata sp. nov., M. forcipata sp. nov., M. ledongensis sp. nov., M. longihamata sp. nov., M. mecobursoides sp. nov., M. microdonta sp. nov., M. papillisetosa sp. nov., M. parallela sp. nov., M. recticostata sp. nov., M. segregnatha sp. nov., M. sinuicosta sp. nov., M. taiwanensis sp. nov. and M. taeniophylla sp. nov. The male of M. flavilineata Kitajima et Sakamaki, 2019 is described for the first time. Images of both adults and genitalia are provided. All species are divided into two species-groups, and a key to each group as well as a map showing the distribution of each group in China are given.


Assuntos
Lepidópteros , Mariposas , Distribuição Animal , Animais , China , Genitália
7.
Zootaxa ; 4890(1): zootaxa.4890.1.2, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33311243

RESUMO

The maculosa species-group of the genus Promalactis Meyrick, 1908 is proposed based on the features of the forewing pattern. Members of this group have a white speckle in the forewing cell and usually with more than eight white markings distributed mostly at or above the fold and along the dorsal margin. Eighteen new species are described: P. coloristigmosa sp. nov., P. cruciata sp. nov., P. lateridentalis sp. nov., P. latispinata sp. nov., P. lonchodes sp. nov., P. magnispina sp. nov., P. plicata sp. nov., P. bomiensis sp. nov., P. fasciserrata sp. nov., P. brevipalpa sp. nov., P. libona sp. nov., P. medilargissima sp. nov., P. ramivalvata sp. nov., P. aculeiformis sp. nov., P. apicitriangula sp. nov., P. curvispinosa sp. nov., P. medimacularis sp. nov., and P. serraticostalis sp. nov. Photographs of adults and genitalia of the new species are provided. A total of 50 species of the maculosa species-group are listed, including 32 previously known species.


Assuntos
Lepidópteros , Mariposas , Distribuição Animal , Animais , Genitália
8.
Biol Open ; 9(12)2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33328190

RESUMO

Mechanisms that enhance energy expenditure are attractive therapeutic targets for obesity. Previously we have demonstrated that mice lacking cd47 are leaner, exhibit increased energy expenditure, and are protected against diet-induced obesity. In this study, we further defined the physiological role of cd47 deficiency in regulating mitochondrial function and energy expenditure in both white and brown adipose tissue. We observed that cd47 deficient mice (under normal chow diet) had comparable amount of white fat mass but reduced white adipocyte size as compared to wild-type mice. Subsequent ex vivo and in vitro studies suggest enhanced lipolysis, and not impaired lipogenesis or energy utilization, contributes to this phenotype. In contrast to white adipose tissue, there were no obvious morphological differences in brown adipose tissue between wild-type and knockout mice. However, mitochondria isolated from brown fat of cd47 deficient mice had significantly higher rates of free fatty acid-mediated uncoupling. This suggests that enhanced fuel availability via white adipose tissue lipolysis may perpetuate elevated brown adipose tissue energy expenditure and contributes to the lean phenotype observed in cd47 deficient mice.

9.
Med Sci Monit ; 26: e926215, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33168797

RESUMO

BACKGROUND Superb microvascular imaging (SMI) is a new blood flow imaging technique used to evaluate microvascular blood flow. This study evaluated whether SMI was superior to color Doppler flow imaging (CDFI) for evaluating placental microcirculation. MATERIAL AND METHODS This prospective study included pregnant women in their third trimester who were evaluated at General Hospital of Hebei Province from February to June 2017. The distribution of vascular patterns, including pulsatility index (PI), resistance index (RI), S/D, time average velocity (TAV), and vessels per unit area, were evaluated by SMI and CDFI. RESULTS This study evaluated 110 pregnant women of mean age 29.53 years. SMI and CDFI yielded statistically significant differences in PI (0.76 vs. 0.62), RI (0.71 vs. 0.47), S/D (2.23 vs. 1.71), TAV (14.35 vs. 22.45), and vessels per unit area (0.26 vs. 0.05) (P<0.001 each). The weight of the pregnant women correlated positively with RI (P=0.048) and negatively with vessels per unit area (P=0.040) as determined by SMI. Weeks of gestation correlated negatively correlated with PI (P=0.008), RI (P=0.004), S/D (P=0.015), and vessels per unit area (P=0.014) by CDFI, and positively with RI (P<0.001) and S/D (P=0.001) by SMI. The results of stratified comparisons of CDFI and SMI based on age, weight, and gestational weeks were consistent overall. CONCLUSIONS SMI, which has a higher rate of placental vascularity, a clearer display of capillaries, a greater sensitivity to low flow, and an advantage in displaying microcirculation of the placenta, can serve as a new and effective method of evaluating placental blood flow.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33051982

RESUMO

AIM: Radium-223, a targeted alpha therapy, is approved widely for the treatment of patients with metastatic castrate-resistant prostate cancer, based on a pivotal phase 3 study in predominantly white patients. We investigated the efficacy and safety of radium-223 in Asian patients with castrate-resistant prostate cancer and metastatic bone disease. METHODS: This multicenter, prospective, single-arm, open-label phase 3 trial evaluated the efficacy and safety of the standard radium-223 regimen (55 kBq/kg every 4 weeks for six cycles) in patients from Asian countries. The primary endpoints were the safety and overall survival. RESULTS: A total of 226 patients were enrolled and received at least one dose of radium-223. Median overall survival was 14.0 months (95% confidence interval [CI], 11.2-17.4). Median time to total alkaline phosphatase and prostate-specific antigen progression were 7.5 (95% CI, 6.8-7.7) and 3.6 (95% CI, 3.1-3.7) months, respectively. Median skeletal-related event-free survival was 26.0 months (95% CI, 12.6-not reached). Grade ≥3 treatment-emergent adverse events were reported in 103 (46%) of 226 patients, with anemia being the most common event (34 [15%] patients). Grade ≥3 drug-related treatment-emergent adverse events occurred in 39 (17%) of 226 patients. Serious treatment-emergent adverse events were reported in 65 (29%) of 226 patients. Seven (3%) patients had an adverse event leading to death; none were considered to be related to radium-223. CONCLUSION: The results of this study support the use of the standard radium-223 regimen for the treatment of Asian patients with castrate-resistant prostate cancer and symptomatic bone metastases.

11.
Zookeys ; 975: 125-157, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33117068

RESUMO

Sixteen new species of the genus Meleonoma Meyrick, 1914 from Hainan Island, China are described: M. apicicurvata Wang, sp. nov., M. apicirectangula Wang, sp. nov., M. bicuspidata Wang, sp. nov., M. bidentata Wang, sp. nov., M. conica Wang, sp. nov., M. hainanensis Wang, sp. nov., M. latiunca Wang, sp. nov., M. linearis Wang, sp. nov., M. magnidentata Wang, sp. nov., M. ornithorrhyncha Wang, sp. nov., M. parilis Wang, sp. nov., M. pectinalis Wang, sp. nov., M. puncticulata Wang, sp. nov., M. quadritaeniata Wang, sp. nov., M. robustispina Wang, sp. nov. and M. rostellata Wang, sp. nov. Images of adult dorsal habitus and genitalia of the new species are provided. A map showing the collecting localities and photos of the habitat where the specimens were collected are provided, along with two maps showing the distribution of each species.

12.
Zootaxa ; 4767(4): zootaxa.4767.4.8, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-33056549

RESUMO

Three new species of the genus Homaloxestis Meyrick are described from China: H. trapezialis sp. nov., H. longicornuta sp. nov., and H. rotundisigna sp. nov. The female of H. miscogana Wu, 1997 is described for the first time. Photographs of adults and genitalia are provided.


Assuntos
Mariposas , Distribuição Animal , Animais , China , Feminino , Genitália
13.
Zootaxa ; 4801(2): zootaxa.4801.2.12, 2020 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-33056667

RESUMO

Two new species of the genus Meleonoma Meyrick, 1914 from Thailand are described: M. segregacantha sp. nov. and M. fornicalis sp. nov. A checklist and a key to all the seven species from Thailand are given. Images of adults and genitalia of the new species are provided.


Assuntos
Mariposas , Distribuição Animal , Animais , Genitália , Tailândia
14.
Zootaxa ; 4838(3): zootaxa.4838.3.2, 2020 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-33056811

RESUMO

Fifteen new species of the genus Meleonoma Meyrick are described: M. anisodonta sp. nov., M. catenata sp. nov., M. dilativalva sp. nov., M. dorsibacilliformis sp. nov., M. lanceolata sp. nov., M. longaedeaga sp. nov., M. longiprocessa sp. nov., M. lunulata sp. nov., M. papillata sp. nov., M. parvissima sp. nov., M. rugulosa sp. nov., M. tenuiuncata sp. nov., M. tianmushana sp. nov., M. triacantha sp. nov. and M. ventrospinosa sp. nov.; one species, M. aridula (Meyrick, 1910), is newly recorded from China; and the female of M. microbyrsa (Wang, 2003) is described for the first time. Images of both adults and genitalia are provided. All species are divided into two species-groups, and a key to each group as well as a map showing the distribution of each group in China are given.


Assuntos
Mariposas , Distribuição Animal , Estruturas Animais , Animais , China , Feminino , Tamanho do Órgão
15.
Zootaxa ; 4838(3): zootaxa.4838.3.3, 2020 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-33056812

RESUMO

Thirteen new species of the genus Stathmopoda are described from China: S. atrifusca sp. nov., S. cellifaria sp. nov., S. cornuta sp. nov., S. digitiprocessa sp. nov., S. dolichantha sp. nov., S. falsistimulata sp. nov., S. guangxiensis sp. nov., S. ingena sp. nov., S. liberata sp. nov., S. purpurata sp. nov., S. serrifasciaria sp. nov., S. similatridorsalis sp. nov. and S. spinicornuta sp. nov. Images of both adults and genitalia of the new species are provided, along with a map showing the distribution of these species.


Assuntos
Mariposas , Distribuição Animal , Animais , China , Genitália
16.
Zootaxa ; 4763(3): zootaxa.4763.3.3, 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33056854

RESUMO

Meleonoma Meyrick, 1914 is treated as a genus of the family Autostichidae based on the recent study. Fifty species are transferred from the genus Cryptolechia to Meleonoma, and eight Meleonoma species groups are proposed for the taxonomic convenience based on both molecular data and morphological study. Eight species groups include: the malacobyrsa group, the segregnatha group, the annulignatha group, the fasciptera group, the jigongshanica group, the dentivalvata group, the facialis group, and the acutiuscula group. Diagnostic characters and representative illustrations of each species group are provided. A discussion on the proposed species groups is given, and a checklist of all the 88 described Meleonoma species is provided.


Assuntos
Mariposas , Distribuição Animal , Animais
17.
Front Oncol ; 10: 1503, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33014793

RESUMO

Aim: Response Evaluation Criteria in Solid Tumors (RECIST) is occasionally insufficient for evaluation. We proposed a new prognostic index (NPI) that combines the standardized uptake value (SUV), metabolic tumor volume (MTV), and RECIST. Methods: In total, 116 patients with lung cancer who underwent consecutive positron emission tomography-computed tomography prior to and after the initial treatment were included. We formulated the NPI by estimating the hazard ratios of overall survival for ΔMTV, ΔSUVmax, and ΔD (tumor size based on RECIST). Progression-free survival (PFS) and overall survival (OS) were compared between RECIST and the NPI. Results: ROC curve analysis identified two cutoff values based on the NPI (≤ -49.3% and ≥43.4%) to discriminate partial remission (NPR), stable disease (NSD) and progressive disease (NPD). Based on RECIST, survival analysis did not discriminate significantly on either PFS or OS between the PR, SD, and PD groups. However, according to the NPI, PFS and OS differed significantly between the NPR, NSD, and NPD groups (training set: PFS, p = 0.048; OS, p = 0.026; validation set: PFS, p = 0.004; OS, p = 0.023). Moreover, therapeutic response based on NPI was independent prognostic factor for both PFS [NPR as reference, NSD: hazard ratio (HR) 2.04; 95% confidence interval (95% CI) 1.35-3.08; p = 0.001; NPD: HR 6.87; 95% CI 3.03-15.57; p < 0.001] and OS (NPR as reference, NSD: HR 1.64; 95% CI 1.05-2.57; p = 0.031; NPD: HR 3.56; 95% CI 1.59-7.95; p = 0.002). Conclusion: The NPI showed superiority for evaluation of the therapeutic response and survival for patients with non-small cell lung cancer, overcoming the limitations of RECIST.

18.
Lung Cancer ; 149: 137-143, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33011375

RESUMO

OBJECTIVES: We retrospectively investigated the prognostic value of FDG-PET performed for patients with Stage ⅢB/IV EGFR-mutant non-small-cell lung cancer (NSCLC) receiving EGFR tyrosine kinase inhibitor (TKI) therapy. METHODS: A total of 78 patients newly diagnosed with Stage ⅢB/IV EGFR-mutant NSCLC who received baseline and interim PET/CT examination and were treated with EGFR-TKI therapy were included. Interim PET was performed after 4-6 weeks of treatment. Cox proportional hazards regression analysis was used to assess the association between quantitative 18F-FDG PET/CT parameters, other clinicopathological factors and progression-free survival (PFS), non-durable clinical benefit (non-DCB). Five interim PET variables were analyzed in this study in the prediction of non-DCB. RESULTS: The one-year and two-year progression-free survival rates of the patients were 33.9% (28.6-39.2%) and 20.7% (16.1-25.3%), respectively. Multivariable analysis indicated that interim PET relevant factors ΔSUVmax (p = 0.002, p = 0.014) and ΔSUVmean (p = 0.000, p = 0.030) were independent risk factors for predicting the PFS or non-DCB of patients receiving EGFR-TKI treatment. The optimal cutoff values of the parameters in the tumor survival analyses were 56.74% for ΔSUVmax (p = 0.002) and 36.48% for ΔSUVmean (p = 0.001). ΔSUVmax had the highest diagnostic value in the prediction of non-DCB. The one-year progression-free survival rates (95% confidence intervals) of patients with ΔSUVmax ≥ 56.74% and ΔSUVmax <56.74% were 59.5% (44.2-74.8%) and 5.7% (0.0-13.3%), respectively (p = 0.000). CONCLUSION: An early PET scan after 4-6 weeks can effectively predict the PFS and non-DCB of patients with Stage ⅢB/IV EGFR-mutant NSCLC receiving EGFR-TKI therapy.

19.
J Diabetes Res ; 2020: 6873891, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33029536

RESUMO

Background: An estimated 425 million people globally have diabetes, accounting for 12% of the world's health expenditures, and the number continues to grow, placing a huge burden on the healthcare system, especially in those remote, underserved areas. Methods: A total of 584,168 adult subjects who have participated in the national physical examination were enrolled in this study. The risk factors for type II diabetes mellitus (T2DM) were identified by p values and odds ratio, using logistic regression (LR) based on variables of physical measurement and a questionnaire. Combined with the risk factors selected by LR, we used a decision tree, a random forest, AdaBoost with a decision tree (AdaBoost), and an extreme gradient boosting decision tree (XGBoost) to identify individuals with T2DM, compared the performance of the four machine learning classifiers, and used the best-performing classifier to output the degree of variables' importance scores of T2DM. Results: The results indicated that XGBoost had the best performance (accuracy = 0.906, precision = 0.910, recall = 0.902, F-1 = 0.906, and AUC = 0.968). The degree of variables' importance scores in XGBoost showed that BMI was the most significant feature, followed by age, waist circumference, systolic pressure, ethnicity, smoking amount, fatty liver, hypertension, physical activity, drinking status, dietary ratio (meat to vegetables), drink amount, smoking status, and diet habit (oil loving). Conclusions: We proposed a classifier based on LR-XGBoost which used fourteen variables of patients which are easily obtained and noninvasive as predictor variables to identify potential incidents of T2DM. The classifier can accurately screen the risk of diabetes in the early phrase, and the degree of variables' importance scores gives a clue to prevent diabetes occurrence.

20.
Cancer Cell Int ; 20: 412, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32863771

RESUMO

Background: Endometrial cancer (EC) is a common malignancy of the female reproductive system. Circular RNAs (circRNAs) were demonstrated to exert critical roles in cancers, including EC. This study aimed to investigate the effects of hsa_circRNA_0001776 (circ_0001776) on EC. Methods: Real-time quantitative PCR (RT-qPCR) was used to measure circ_0001776, microRNA-182 (miR-182) and leucine-rich repeats and immunoglobulin-like domains 2 (LRIG2) expression. The diagnostic and prognostic values of circ_0001776 were identified by receiver operating characteristic (ROC) curve analysis and survival analysis, respectively. RNase R digestion was used to characterize circ_0001776, and the localization of circ_0001776 was evaluated by cell fractionation assay. Then, cell counting kit-8 (CCK-8), colony formation, and flow cytometry analysis were used to detect cell proliferation and apoptosis, respectively. The real-time glycolytic rate (ECAR) and lactate production were measured by extracellular flux analysis and a lactate assay kit, respectively. Bioinformatics analysis and dual-luciferase reporter assay were used to determine the interaction among circ_0001776, miR-182 and LRIG2. The protein expression of LRIG2 was determined by western blot. Moreover, circ_0001776 overexpression vector was used to upregulate circ_0001776 expression in an animal tumor model. Results: Circ_0001776 and LRIG2 were downregulated, while miR-182 was upregulated in EC tissues and cells. Low expression of circ_0001776 was correlated with the 5-year survival rate of EC patients. Upregulated circ_0001776 markedly attenuated cell proliferation and glycolysis, and enhanced cell apoptosis. Besides, circ_0001776 sponged miR-182 to regulate LRIG2 expression. Circ_0001776 could suppress EC progression by miR-182/LRIG2 axis. Furthermore, we also found that circ_0001776 significantly inhibited tumor growth in vivo. Conclusion: Our results confirmed that circ_0001776 inhibited EC tumorigenesis and progression via miR-182/LRIG2 axis, providing a potential therapeutic target for EC.

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