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1.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(2): 353-361, 2020 Apr 18.
Artigo em Chinês | MEDLINE | ID: mdl-32306022

RESUMO

OBJECTIVE: The incidence of colorectal stromal tumor is low among digestive tract tumors, therefore the literatures about clinicopathological features and prognosis of colorectal stromal tumor are few at home and abroad. In this study, we performed survival analyses for colorectal stromal tumor. The nomogram made by prognostic factors provided basis for evaluation of prognosis. METHODS: The clinico-pathological and prognostic data of colorectal stromal tumor between January 1992 and December 2015 were collected from the surveillance, epidemiology, and end results (SEER) database. The survival analyses were made by SPSS 24.0 software. The nomogram and calibration curve were made by RMS package in R 3.5.2 software. RESULTS: In the study, 546 patients with colorectal stromal tumor were included. The median age of onset was 64 years. The regional lymph node metastasis (LNM) rate was 9.4%. The multivariate Cox regression analyses of the 546 cases showed that the older age of onset (>64 years), single or divorce, colon tumor (compared with rectal tumor), non-surgery, high histological grade, LNM and distant metastasis were associated with worse cancer specific survival (CSS) and overall survival (OS), P < 0.05 for all. The treatment district was independent prognostic factor of OS (P = 0.027). The C-index of independent prognostic factors predicting CSS and OS probability were 0.76 (95%CI: 0.72-0.80) and 0.75 (95%CI: 0.72-0.78), respectively. Multivariate analyses were further carried out in the 174 patients with definite histological grade and tumor location, which revealed that the age of onset, histological grade, surgery or not were independent prognostic factors of CSS and OS (P < 0.05 for all). Tumor location was associated with CSS (P = 0.041) but not OS (P = 0.057) among the 174 cases. Four independent prognostic factors influencing the 174 patients' prognosis were used to make nomogram for predicting survival probability of 546 cases. The C-index of four prognostic factors predicting probability of CSS and OS of the 546 cases were separately 0.71 (95%CI: 0.66-0.75) and 0.73 (95%CI: 0.70-0.77). The nomogram had more accuracy for predicting OS probability of colorectal stromal tumors. CONCLUSION: The prognosis of colorectal stromal tumor was affected by multiple clinicopathological factors. The nomogram provided the basis for predicting the survival probability of patients with colorectal stromal tumor.

2.
Eur Rev Med Pharmacol Sci ; 23(23): 10332-10342, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31841213

RESUMO

OBJECTIVE: MicroRNAs (miRNAs) have been demonstrated to be involved in the pathogenesis of various human cancers, including oral squamous cell carcinoma (OSCC). Here, we designed this study to explore the potential effect of miR-1290 on tumorigenesis of OSCC. PATIENTS AND METHODS: The expressions of miR-1290 and cyclin G2 (CCNG2) in OSCC were observed by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Dual-Luciferase Reporter Assay was performed to confirm the relationship between miR-1290 and CCNG2. The functions of miR-1290 and CCNG2 were analyzed using transwell assay. The Western blot analysis was used to detect epithelial-mesenchymal transition (EMT). RESULTS: Upregulation of miR-1290 and downregulation of CCNG2 were identified in OSCC. And upregulation of miR-1290 was associated with clinicopathological characteristics and poor prognosis in OSCC patients. Moreover, the downregulation of miR-1290 inhibited cell metastasis and EMT in OSCC cells. Furthermore, CCNG2 was a direct target of miR-1290. Its expression was inversely regulated by miR-1290 in OSCC cells. At the same time, the suppressive effect of CCNG2 was observed in OSCC. Furthermore, overexpression of CCNG2 weakened the promoted effect of miR-1290 on cell metastasis in OSCC. CONCLUSIONS: MiR-1290 promoted cell metastasis and EMT, inhibiting CCNG2 expression in OSCC.

3.
QJM ; 112(12): 927-928, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31432085
4.
Zhonghua Fu Chan Ke Za Zhi ; 54(5): 293-300, 2019 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-31154709

RESUMO

Objective: To investigate the prevalence of high-risk HPV subtypes in different pathological types of cervical cancer, and analyze the attribution of carcinogenic HPV subtypes in different pathological types. Methods: A total of 1 541 patients with cervical cancer were treated between February 2009 and October 2016 in Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College. The median age at diagnosis was 49 years (ranged 20-82 years old). The numbers of patients with cervical cancer from North China, Northeast China, East China, Central China and other regions (including Northwest, Southwest and South China) were 961, 244, 175, 87 and 74 cases, respectively. Pathological types: 1 337 cases of squamous cell carcinoma (SCC), 87 usual adenocarcinoma (ADC), 23 adenosquamous carcinoma (ASC), 20 mucinous carcinoma (MC), 19 clear cell carcinoma (CCC), 12 endometrioid carcinoma (EC), 25 neuroendocrine carcinoma (NEC), 9 serous carcinoma (SC), 5 villous adenocarcinoma (VADC) and 4 minimal deviation adenocarcinoma (MDAC). The prevalence of high-risk HPV in different regions, age groups at diagnosis and pathological types in cervical cancer were analyzed. The attribution of 13 high-risk HPV subtypes in different pathological types of cervical cancer based on proportional attribution method, and the attribution of high-risk HPV subtypes prevented by 9-valent HPV vaccine in SCC and ADC were calculated. Results: (1) The prevalence of high-risk HPV in 1 541 patients with cervical cancer was 86.6% (1 335/1 541). The multiple high-risk HPV infection rate in patients with SCC ≥60 years old (23.0%, 37/161) was significantly higher than those in patients aged 45-59 years old and ≤44 years old [11.4% (85/747) vs 11.7% (50/429), P<0.01], and the high-risk HPV infection rates of patients with cervical cancer in North China, Northeast China, East China, Central China and other regions were respectively 86.8% (834/961), 87.7% (214/244), 83.4% (146/175), 83.9% (73/87) and 91.9% (68/74). SCC (86.8%, 1 337/1 541) and ADC (5.6%, 87/1 541) were the most common pathological types in cervical cancer. The high-risk HPV prevalence of SCC, ADC, ASC, MC, NEC and VADC were 90.1% (1 205/1 337), 74.7% (65/87), 87.0% (20/23), 65.0% (13/20), 72.0% (18/25) and 5/5 respectively. The high-risk HPV infection rates of SC, EC, CCC and MDAC were 4/9, 3/12, 2/19 and 0/4 respectively. (2) According to proportional attribution, HPV 16 (69.5%), HPV 18 (5.6%), HPV 58 (2.2%), HPV 31 (1.9%), HPV 52 (1.4%) and HPV 33 (1.3%) were the six common high-risk HPV subtypes in SCC. While, HPV 18 (44.1%), HPV 16 (20.5%), HPV 52 (2.3%), HPV 58 (1.2%) and HPV 51 (1.2%) were the main carcinogenic subtypes in ADC. The main carcinogenic high-risk HPV subtypes of ASC, NEC and MC were HPV 18 and HPV 16. The total attribution of HPV 16, 18, 31, 33, 45, 52 and 58 prevented by 9-valent HPV vaccine in SCC and ADC were 82.6% and 68.1% respectively; the attribution of HPV 45 in SCC and ADC were only 0.8% and 0. Conclusions: SCC and ADC are the main pathological types in cervical cancer. SCC, ADC, ASC, MC, NEC and VADC are closely related to high-risk HPV infection. HPV 16 is the main carcinogenic genotypes of SCC. HPV 18 maybe play an important role in the pathogenesis of ADC.


Assuntos
Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/epidemiologia , Neoplasias do Colo do Útero/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Feminino , Humanos , Pessoa de Meia-Idade , Papillomaviridae/classificação , Papillomaviridae/genética , Infecções por Papillomavirus/virologia , Prevalência , Neoplasias do Colo do Útero/virologia , Adulto Jovem
5.
Eur Rev Med Pharmacol Sci ; 23(10): 4382-4390, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31173312

RESUMO

OBJECTIVE: To study the efficacy of helmet-assisted non-invasive ventilation and conventional ventilation in the treatment of acute respiratory failure (ARF). MATERIALS AND METHODS: Cochrane Library, PubMed, Embase and CNKI databases were searched for randomized controlled trials and case-control trials of helmet-assisted noninvasive ventilation in the treatment of ARF. The outcome measures included respiratory rate, intubation rate, complication rate, mortality rate and arterial blood gas analysis of the commonly used indicators (PaCO2/ PaO2 / pH). The results of the included studies' odds ratio (OR) and its 95% confidential interval (CI) were analyzed using Stata software. RESULTS: The results of the analysis showed that the in-hospital mortality, intubation rate and complication rate were all significantly decreased with the p-value less than 0.05, which was statistically significant. CONCLUSIONS: Helmet-assisted noninvasive ventilation can significantly reduce hospital mortality, intubation rate and complication rate, improving the survival rate and prognosis of patients with ARF.

6.
Clin Radiol ; 74(7): 569.e9-569.e17, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30967244

RESUMO

AIM: To analyse the correlation between imaging features using multiple techniques and extracellular mucus content in pure mucinous breast carcinoma (PMBC). MATERIALS AND METHODS: A retrospective review of available images from 25 patients with 25 PMBC tumours was conducted, with ultrasonography (US), ultrasonic elastography (USE), mammography, and breast-specific gamma imaging (BSGI) available for 25, 15, 11, and eight patients, respectively. Microscopic slides from each tumour were evaluated for extracellular mucus content. The correlation between imaging features and mucus content was analysed using linear-by-linear association chi-square tests or Spearman's rank correlation analyses. RESULTS: On US images, a significant correlation was found between mucus content and echo pattern (p=0.042) and colour Doppler blood flow (p=0.032), with a trend that the lower mucus content present in tumours, the more likely they were detected with isoechoic echo and high blood flow. On USE images, a moderate negative correlation (r=-0.60, p=0.029) was observed between mucus content and tumour stiffness. On BSGI images, a strong negative correlation (r=-0.92, p=0.001) was shown between mucus content and lesion to non-lesion ratio (L/N) values of radioactivity counts. No significant correlation was found between mucus content and mammography imaging features (all p>0.05). CONCLUSION: Imaging features at US, USE, and BSGI correlated with extracellular mucus content in PMBC tumours, among which the L/N value using BSGI imaging is the most relevant feature.

7.
Neoplasma ; 66(2): 261-270, 2019 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-30784280

RESUMO

GRK5 is a multifunctional protein that is able to move within the cell in response to various stimuli to regulate key intracellular signaling from receptor activation, on plasmamembrane, to gene transcription, in the nucleus. Thus, GRK5 is involved in the development and progression of several pathological conditions including cancer. Here, we report an important tumor-promoting role for GRK5 in renal cell carcinoma (RCC). We investigated the expression pattern, clinical significance, and function of GRK5 in RCC. By using quantitative real-time polymerase chain reaction (qRT-PCR) and tissue microarray (TMA) immunohistochemistry (IHC), we first demonstrated that compared with paired adjacent nontumor (NT) tissues, RCC tissues presented with higher GRK5 expression. Moreover, we found that GRK5 upregulation was associated with poor clinical outcomes in RCC patients. In vitro, we found that GRK5 knockdown reduced viability, invasive ability, migratory ability, and decreased proportion of cells in S phase, with concomitant increase in G1 phase in RCC cell lines, while GRK5 overexpression promoted tumor cell proliferation, cell invasion, migration and increased proportion of cells in S phase, with concomitant decrease in G1 phase. Collectively, our findings describe the tumour-promoting role of GRK5 in RCC and thus provide molecular evidence for new therapeutic options in RCC.


Assuntos
Carcinoma de Células Renais/patologia , Quinase 5 de Receptor Acoplado a Proteína G/genética , Neoplasias Renais/patologia , Movimento Celular , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos
8.
Phys Rev Lett ; 122(2): 024801, 2019 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-30720300

RESUMO

A new relativistic vortex cutter driven by the Laguerre-Gaussian (LG) mode is carried out for the first time in three-dimensional particle-in-cell simulations. Studies show that the electric fields periodically concentrate and emanate within every laser wavelength for the reflected circularly polarized LG_{p}^{l} (p=0, l=1, σ_{z}=-1) laser, which works just like a vortex cutter, resulting in a relativistic ultrashort collimated electron cluster with a constant period in space. A single particle model is given and verifies that the cluster formation has a close relation with the parameters of orbital angular momentum (l) and spin angular momentum (σ_{z}). Such a relativistic vortex cutter potentially can be applied for the accelerator, generating high-flux particle and coherent radiation sources, and so on.

9.
Zhonghua Yi Xue Za Zhi ; 99(7): 491-495, 2019 Feb 19.
Artigo em Chinês | MEDLINE | ID: mdl-30786344

RESUMO

Objective: To establish automatic liver fibrosis classification models by using traditional machine learning and deep learning methods and preliminaryly evaluate the efficiency. Methods: Gray scale ultrasound images and corresponding elastic images of 354 patients, 247 males and 107 females, mean age (54±12) years undergoing partial hepatectomy in Zhongshan Hospital of Fudan University from November 2014 to January 2016 were enrolled in this study. By using traditional machine learning and deep learning methods, an automatic classification model of liver fibrosis stages (S0 to S4) were established through feature extraction and classification of ultrasound image data sets and the accuracy in different classification categories of each model were calculated, by using liver biopsy as the reference standard. Results: Pathological examination showed 73 cases in pathological stage S0, 40 cases in S1, 49 cases in S2, 41 cases in S3, and 151 cases in S4. The traditional machine classification model based on support vector machine (SVM) classifier and sparse representation classifier and the deep learning classification model based on LeNet-5 neural network, their accuracy rates in the two categories (S0/S1/S2 and S3/S4) were 89.8%, 91.8% and 90.7% respectively; the accuracy rates in the three categories (S0/S1 and S2/S3 and S4) were 75.3%, 79.4% and 82.8% respectively; the accuracy in the three categories (S0 and S1/S2/S3 and S4) were 79.3%, 82.7% and 87.2% respectively. Conclusions: Computer-aided assessment of liver fibrosis progression in patients with chronic hepatitis B has a high accuracy, and can achieve a more detailed classification. This method is expected to be applied in the non-invasive evaluation of liver fibrosis in patients with hepatitis B in clinical work in the future.


Assuntos
Hepatite B Crônica , Cirrose Hepática , Adulto , Idoso , Progressão da Doença , Feminino , Fibrose , Hepatite B Crônica/complicações , Humanos , Fígado , Cirrose Hepática/etiologia , Masculino , Pessoa de Meia-Idade , Ultrassonografia
10.
Artigo em Chinês | MEDLINE | ID: mdl-29996252

RESUMO

Objective: To know the incidence of heat stroke and explore it's prediction model in Pudong New Area of Shanghai. Methods: An epidemiological investigation was conducted on heat stroke cases in Pudong New Area of Shanghai from 2013 to 2017. Daily temperature data during this period were collected to explore it's influence. Results: 246 heat stroke cases were reported and investigated, 70.3% (173/246) of them were male. 170 cases are severe heat stroke, accounting for 69.1%. 28 patients died, accounting for 11.4% of all cases of heat stroke, and 16.5% (28/170) of severe heat stroke cases. Thermoplegia (56.5%, 96/170) was the most popular type among severe heat stroke cases. Heat prostration, heat cramps and mixed type account for 17.1% (29/170) , 12.4% (21/170) and 14.0% (24/170) respectively. Scatter plot and linear regression demonstrated that there was a significant linear relation between number of high temperature days and number of heat stroke cases (P<0.01) . And the prediction model is: Predictive number of annual heat stroke cases=ß×Number of annual high temperature days+Intercept. Leave-one-out cross validation result shows that the predictive number of annual heat stroke cases from 2013 to 2017 were 85.7%, 90.9%, 83.3%, 91.9 and 84.3% respectively. Conclusion: There was a significant linear relation between number of high temperature days and number of heat stroke cases in Pudong New Area. The related work arrangement for heat stroke prevention could be well planed according to the prediction model.


Assuntos
Golpe de Calor/epidemiologia , Temperatura Alta , China , Humanos , Incidência , Masculino , Temperatura
11.
Zhonghua Yi Xue Za Zhi ; 98(15): 1148-1151, 2018 Apr 17.
Artigo em Chinês | MEDLINE | ID: mdl-29690726

RESUMO

Objective: To evaluate the diagnostic value of two-dimensional shear wave elastography(2D-SWE)in the assessment of hepatic fibrosis in a large-scale clinical setting. Methods: A total of 1 079 subjects who underwent partial hepatectomy were examined with 2D-SWE to measure liver stiffness before operation and the liver stiffness measurements were compared with histologic findings.The published cutoff values of liver stiffness examined with 2D-SWE were used as a reference, as 7.2, 9.2, and 10.4 kPa for diagnosing substantial fibrosis(S2), severe fibrosis(S3), and cirrhosis(S4), respectively.The area under curve(AUC)was calculated to verify the value of 2D-SWE in the evaluation of hepatic fibrosis. Results: Compared with the pathological diagnosis, the AUC of 2D-SWE in diagnosing substantial fibrosis(S2), severe fibrosis(S3), and cirrhosis(S4)were 0.976, 0.981, and 0.968, the sensitivity were 95.3%, 92.6%, and 93.5%, the specificity were 87.8%, 87.5% and 89.3%, and the accuracy were 88.8%, 90.0% and 91.0%, respectively(P<0.05). Conclusion: 2D-SWE is an effective imaging tool for noninvasive diagnosis of hepatic fibrosis.


Assuntos
Técnicas de Imagem por Elasticidade , Cirrose Hepática , Hepatectomia , Humanos , Fígado , Reprodutibilidade dos Testes
12.
Zhonghua Wai Ke Za Zhi ; 55(12): 894-897, 2017 Dec 01.
Artigo em Chinês | MEDLINE | ID: mdl-29224262

RESUMO

AJCC Esophageal Cancer Staging System, 8(th) edition will be implemented on January 1, 2018. The N staging in 8(th) edition of staging system remains following 7(th) edition based on the number of metastatic nodes, except the limited revision of the regional lymph node map. N staging revision was reviewed from the simple definition of negative (N0) and positive (N1) lymph node(s) to the positive node number based proposal (7(th) edition). The 7(th) edition staging system, especially the N staging, were proved with more advantages on distinguishing disease progression and predicting prognosis of the esophageal cancer. On other hand, the disadvantages of 7(th) edition N staging are discussed. The refined N staging based on the number of metastatic node station is introduced. The extent and station of metastatic node could better reflect the disease progression and prognosis according to our research. The controversy on N staging of esophagogastric junction cancer is discussed as well. Other reported N staging associated index including lymph node ratio, lymphatic vessel invasion and biomarkers are reviewed and evaluated.


Assuntos
Neoplasias Esofágicas/diagnóstico , Estadiamento de Neoplasias , Progressão da Doença , Neoplasias Esofágicas/patologia , Junção Esofagogástrica , Humanos , Linfonodos , Metástase Linfática , Prognóstico
13.
Phys Rev Lett ; 119(16): 164801, 2017 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-29099228

RESUMO

We present experimental studies on ion acceleration using an 800-nm circularly polarized laser pulse with a peak intensity of 6.9×10^{19} W/cm^{2} interacting with an overdense plasma that is produced by a laser prepulse ionizing an initially ultrathin plastic foil. The proton spectra exhibit spectral peaks at energies up to 9 MeV with energy spreads of 30% and fluxes as high as 3×10^{12} protons/MeV/sr. Two-dimensional particle-in-cell simulations reveal that collisionless shocks are efficiently launched by circularly polarized lasers in exploded plasmas, resulting in the acceleration of quasimonoenergetic proton beams. Furthermore, this scheme predicts the generation of quasimonoenergetic proton beams with peak energies of approximately 150 MeV using current laser technology, representing a significant step toward applications such as proton therapy.

14.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 31(15): 1164-1167, 2017 Aug 05.
Artigo em Chinês | MEDLINE | ID: mdl-29798350

RESUMO

Objective:To explore the CT, MRI manifestations of the polyp of maxillary sinus cavernous hemangioma. To summarize the imaging characteristics and it may assist doctors to diagnose infarcted angiomatous polyps in maxillary sinus with further developing.Method:A retrospective analysis of the CT, MRI manifestations and clinical analysis about 27 cases confirmed by surgery pathology who are maxillary sinus cavernous hemangioma.Result:Twenty-seven cases were unilateral diseases, and clinical manifestations were nasal congestion. Twenty-seven cases were proceeded CT scan, of which 9 cases of double-phase dynamic enhanced CT examination, 11 cases with MRI examination, of which 6 cases with MRI enhanced examination. CT scan showed unilateral maxillary sinus full of mixed density and involving the nasal cavity, maxillary sinus abnormal expansion of deformation, with local bone resorption damage in 27 cases, but without violation the adjacent tissue. CT enhanced scan of lesions shows that the spotted blood vessels were significantly enhanced and progressive enhancement. MRI can clearly identifylesions and obstructive sinusitis and morbid shape was irregular with mixed signal. T2WI showed high and low mixed ring signals, and the edge of the irregular low signal ring, with enhanced nodules and patchy unevenly enhanced.Conclusion:Most polypof maxillary sinus cavernous hemangioma clinical manifestations are unilateral nasal obstruction. CT shows the maxillary sinus irregular expands and compression bone absorption with a prompt action to this disease.Vascular-like enhancement and progressive enhancement are characteristic enhanced CT manifestations of this disease. MRI can better show the lesion range. T2WI with high and low ring mixed signals, low ring signals, and mostly enhanced nodules, uneven enhancement of plaqueare characteristic imaging manifestations.


Assuntos
Hemangioma Cavernoso/diagnóstico , Seio Maxilar/diagnóstico por imagem , Pólipos Nasais/diagnóstico por imagem , Nariz/diagnóstico por imagem , Humanos , Imagem por Ressonância Magnética , Pólipos , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
15.
Zhonghua Er Ke Za Zhi ; 54(10): 746-751, 2016 Oct 02.
Artigo em Chinês | MEDLINE | ID: mdl-27784476

RESUMO

Objective: To observe hippocampal damage and cognitive impairment of offspring exposed to prenatal maternal seizure induced by amygdala kindling, and to explore the underlying mechanism by the detection of pathological changes of placenta. Method: Adult female SD rats were randomly divided into three groups: control group(8 rats), kindling group(12 rats) and sham group(8 rats). All the rats were allowed to mate after one week's fully kindling. The pregnant rats in kindling group received electric stimulation every 48 h. Dams were allowed to deliver naturally. Effects of maternal seizure on the number of offspring, the survival rate and body weight of pups were observed. HE staining was used to visualize histopathological changes of placenta. Morris water maze test was used to assess the cognitive function and Nissal's staining to detect hippocampal morphology of the offspring. One-way ANOVA analysis and χ2 test were used. Result: Compared with the sham group (95%(78/82)) and the control group (95%(82/86)), the survival rate of pups in kindling group(81%(66/82))was much lower (χ2=13.817, P=0.001). There were no significant differences in the number of pups per litter and pups birth-weight between kindling group and sham group or control group(F=0.312 and 0.257, P=0.736 and 0.776). HE staining showed that placental tissues from control and sham groups were normal whereas the histologic abnormalities of placentas from kindling group were characterized by thickening of the villus vascular walls, luminal stenosis, trophoblasts hyperplasia, abnormalities of trophoblasts with nuclear pyknosis and karyorrhexis and accumulation of inflammatory lymphocytes in labyrinthine zone. Nissl staining showed that neurons in hippocampus of P0(0 d after birth) and P84(84 d after birth) offspring from control and sham groups were normal, but neuronal damages were obvious in hippocampus of P0 and P84 offspring from kindling groups, and the damages in P0 pups were severe with a marked loss of neuron, shrinkage of cells and nuclear pyknosis and karyorrhexis. In the Morris water maze, compared with the sham group ((29±8), (19±9), (10±4)s) and the control group ((25±6), (17±5), (14±4)s) rats in the kindling group ((36±8), (29±8), (30±11)s) exhibited significantly longer escape latency from the 3rd, 4th, and 5th days (F=6.276, 7.518, 18.422, P=0.030, 0.003, 0.000), significant less time in the target quadrant ((27±8) vs.(58±11)and(68±13)s, F=35.993, P=0.000) and reduced number of crossing the platform ((4.4±1.7) vs. (7.2±1.6) and (8.5±1.3)times, F=18.377, P=0.000). In addition, there was no significant difference between control and sham groups(P all >0.05). Conclusion: The prenatal maternal seizures induced significant pathological damages to hippocampus and cognitive impairment of offspring. Hypoxia-ischemia of placenta might play an important role in this process.


Assuntos
Cognição , Hipocampo/patologia , Convulsões/complicações , Animais , Feminino , Excitação Neurológica , Masculino , Neurônios , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Ratos , Ratos Sprague-Dawley
16.
Zhonghua Gan Zang Bing Za Zhi ; 24(4): 270-4, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-27470625

RESUMO

OBJECTIVE: To investigate the correlation between contrast-enhanced ultrasound parameters and hepatic venous pressure gradient (HVPG), and to develop a new noninvasive method for the evaluation of portal hypertension in patients with decompensated liver cirrhosis. METHODS: One-hundred patients with decompensated liver cirrhosis were examined by contrast-enhanced ultrasound, and the dynamic images were collected for offline analysis. The contrast arrival time was obtained in the hepatic artery (HA), portal vein (PV), and hepatic vein (HV), and HA-HV transit time (HA-HVTT) and PV-HV transit time (PV-HVTT) were calculated. At the same time, HVPG was measured within 24 hours after contrast-enhanced ultrasound, Pearson correlation analysis was performed between each parameter and HVPG, and the receiver operating characteristic (ROC) curve was also used for analysis. RESULTS: HV arrival time (HVAT), HA-HVTT, and PV-HVTT were negatively correlated with HVPG (r = -0.385, -0.409, and -0.572, respectively). The area under the ROC curve (AUROC) was 0.903 for PV-HVTT < 2.5 s in judging HVPG≥ l0 mmHg in patients with decompensated liver cirrhosis, and the sensitivity and specificity were 74.4% and 89.5%, respectively. The AUROC was 0.861 for PV-HVTT < 1.5 s in judging HVPG≥l6 mmHg in these patients, and the sensitivity and specificity were 80.4% and 81.5%, respectively. CONCLUSIONS: HVAT and intrahepatic transit time demonstrate negative linear correlations with HVPG in patients with decompensated liver cirrhosis, and among all parameters, PV-HVTT shows the strongest correlation with HVPG and can be used to determine and predict the severity of portal hypertension.


Assuntos
Hipertensão Portal/diagnóstico por imagem , Cirrose Hepática/diagnóstico por imagem , Ultrassonografia , Meios de Contraste , Artéria Hepática/diagnóstico por imagem , Veias Hepáticas/diagnóstico por imagem , Humanos , Pressão na Veia Porta , Veia Porta/diagnóstico por imagem , Curva ROC , Sensibilidade e Especificidade
17.
Pharmazie ; 71(5): 252-7, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27348968

RESUMO

Glabridin, a polyphenolic flavonoid from licorice, has inspired great interest for its antioxidant, anti-inflammatory and skin-lightening activities. However, low water solubility and poor stability of glabridin impedes its topical application in cosmetic products and therapies of dermal diseases. The purpose of this study was to develop a nanosuspension formulation of glabridin to improve its skin permeation. Glabridin nanosuspensions were prepared using anti-solvent precipitation-homogenization method, and Box-Behnken design was adopted to investigate the effects of crucial formulation variables on particle size and to optimize the nanosuspension formulation. The optimal formulation consisted of 0.25% glabridin, 0.47% Poloxamer 188 and 0.11% Polyvinylpyrrolidone K30, and the obtained nanosuspension showed an average particle size of 149.2 nm with a polydispersity index of 0.254. Furthermore, the nanosuspension exhibited significantly enhanced drug permeation flux of glabridin through rat skin with no lag phase both in vitro and in vivo, compared to the coarse suspension and physical mixture. The glabridin nanosuspension showed no significant particle aggregates and a drug loss of 5.46% after storage for 3 months at room temperature. With its enhanced skin penetration, the nanosuspension might be a more preferable formulation for topical administration of poorly soluble glabridin.


Assuntos
Portadores de Fármacos/química , Isoflavonas/administração & dosagem , Nanopartículas , Fenóis/administração & dosagem , Absorção Cutânea , Administração Cutânea , Animais , Química Farmacêutica/métodos , Composição de Medicamentos/métodos , Estabilidade de Medicamentos , Armazenamento de Medicamentos , Isoflavonas/química , Isoflavonas/farmacocinética , Masculino , Tamanho da Partícula , Fenóis/química , Fenóis/farmacocinética , Poloxâmero/química , Povidona/química , Ratos , Ratos Sprague-Dawley , Solubilidade , Suspensões
18.
Clin Radiol ; 71(6): 576-82, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27040802

RESUMO

AIM: To explore the optimal vascular diameter and blood flow measured by ultrasound to predict fistula maturity in Chinese patients. MATERIALS AND METHODS: One hundred and thirty-two patients were enrolled in the study. The blood flow and the diameters of the brachial artery, radial artery, and cephalic vein were analysed according to clinical outcomes. The performances of blood flow, cephalic vein diameter, and the two factors combined in predicting fistula maturity were evaluated and the optimal cut-off values for Chinese patients were determined. RESULTS: During a 6 month follow-up, 113 fistulas were classified as mature and 19 fistulas were classified as failure. In both groups, the diameters of the brachial and radial arteries increased gradually within 2 weeks post-surgery (p<0.05), and cephalic vein diameter and blood flow increased within 4 weeks post-surgery (p<0.05). The blood flow of the failure group was significantly lower than that of the mature group from the first day after creation. The area under the receiver operating characteristic (ROC) curve was 0.95 (529 ml/min, the optimal cut-off value) for blood flow, 0.83 (5.2 mm) for cephalic vein diameter, and 0.96 for the two factors combined in predicting fistula maturity. CONCLUSIONS: For Chinese patients, blood flow >529 ml/min could be used to predict fistula maturity and cephalic vein diameter >5.2 mm could be a supplement when necessary.


Assuntos
Anastomose Cirúrgica/métodos , Artéria Radial/diagnóstico por imagem , Diálise Renal/métodos , Ultrassonografia Doppler em Cores/métodos , Veias/diagnóstico por imagem , Velocidade do Fluxo Sanguíneo , China , Sobrevivência de Enxerto , Humanos , Masculino , Pessoa de Meia-Idade , Artéria Radial/fisiopatologia , Artéria Radial/cirurgia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Veias/fisiopatologia , Veias/cirurgia
19.
Braz. j. med. biol. res ; 49(3): e5043, Mar. 2016. graf
Artigo em Inglês | LILACS | ID: lil-771931

RESUMO

Ovarian cancer is one of the most common causes of death from gynecologic tumors and is an important public health issue. Ghrelin is a recently discovered bioactive peptide that acts as a natural endogenous ligand of the growth hormone secretagogue receptor (GHSR). Several studies have identified the protective effects of ghrelin on the mammalian reproductive system. However, little research has been done on the effects of ghrelin on ovarian cancer cells, and the underlying mechanisms of these effects. We sought to understand the potential involvement of mitogen-activated protein kinases (MAPKs) in ghrelin-mediated inhibition of growth of the ovarian line HO-8910. We applied different concentrations of ghrelin and an inhibitor of the ghrelin receptor (D-Lys3-GHRP-6) to HO-8910 cells and observed the growth rate of cells and changes in phosphorylation of the MAPKs ERK1/2, JNK and p38. We discovered that ghrelin-induced apoptosis of HO-8910 cells was though phosphorylated ERK1/2, and that this phosphorylation (as well as p90rsk phosphorylation) was mediated by the GHSR. The ERK1/2 pathway is known to play an essential part in the ghrelin-mediated apoptosis of HO-8910 cells. Hence, our study suggests that ghrelin inhibits the growth of HO-8910 cells primarily through the GHSR/ERK pathway.


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Regulação Neoplásica da Expressão Gênica/genética , Grelina/farmacologia , Sistema de Sinalização das MAP Quinases/fisiologia , Neoplasias Ovarianas/genética , Apoptose/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Oligopeptídeos/metabolismo , Neoplasias Ovarianas/metabolismo , Fosforilação/efeitos dos fármacos , Receptores de Grelina/antagonistas & inibidores , Receptores de Grelina/metabolismo , Células Tumorais Cultivadas
20.
Braz J Med Biol Res ; 49(3)2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26840702

RESUMO

Ovarian cancer is one of the most common causes of death from gynecologic tumors and is an important public health issue. Ghrelin is a recently discovered bioactive peptide that acts as a natural endogenous ligand of the growth hormone secretagogue receptor (GHSR). Several studies have identified the protective effects of ghrelin on the mammalian reproductive system. However, little research has been done on the effects of ghrelin on ovarian cancer cells, and the underlying mechanisms of these effects. We sought to understand the potential involvement of mitogen-activated protein kinases (MAPKs) in ghrelin-mediated inhibition of growth of the ovarian line HO-8910. We applied different concentrations of ghrelin and an inhibitor of the ghrelin receptor (D-Lys3-GHRP-6) to HO-8910 cells and observed the growth rate of cells and changes in phosphorylation of the MAPKs ERK1/2, JNK and p38. We discovered that ghrelin-induced apoptosis of HO-8910 cells was though phosphorylated ERK1/2, and that this phosphorylation (as well as p90rsk phosphorylation) was mediated by the GHSR. The ERK1/2 pathway is known to play an essential part in the ghrelin-mediated apoptosis of HO-8910 cells. Hence, our study suggests that ghrelin inhibits the growth of HO-8910 cells primarily through the GHSR/ERK pathway.


Assuntos
Regulação Neoplásica da Expressão Gênica/genética , Grelina/farmacologia , Sistema de Sinalização das MAP Quinases/fisiologia , Neoplasias Ovarianas/genética , Apoptose/efeitos dos fármacos , Feminino , Humanos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Pessoa de Meia-Idade , Oligopeptídeos/metabolismo , Neoplasias Ovarianas/metabolismo , Fosforilação/efeitos dos fármacos , Receptores de Grelina/antagonistas & inibidores , Receptores de Grelina/metabolismo , Células Tumorais Cultivadas
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