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1.
Fish Shellfish Immunol ; 133: 108544, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36646339

RESUMO

Aquatic viruses can spread rapidly and widely in seawater for their high infective ability. Polyinosinic-polycytidylic acid (Poly I:C), a viral dsRNA analog, is an immunostimulant that has been proved to activate various immune responses of immune cells in invertebrate. Hemolymph is a critical site that host immune response in invertebrates, and its transcriptome information obtained from Amphioctopus fangsiao stimulated by Poly I:C is crucial for understanding the antiviral molecular mechanisms of this species. In this study, we analyzed gene expression data in A. fangsiao hemolymph tissue within 24 h under Poly I:C stimulation and found 1082 and 299 differentially expressed genes (DEGs) at 6 and 24 h, respectively. Union set (1,369) DEGs were selected for subsequent analyses. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were carried out for identifying DEGs related to immunity. Several significant immune-related terms and pathways, such as toll-like receptor signaling pathways term, inflammatory response term, TNF signaling pathway, and chemokine signaling pathway were identified. A protein-protein interaction (PPI) network was constructed for examining the relationships among immune-related genes. Finally, 12 hub genes, including EGFR, ACTG1, MAP2K1, and other nine hub genes, were identified based on the KEGG enrichment analysis and PPI network. The quantitative RT-PCR (qRT-PCR) was used to verify the expression profile of 12 hub genes. This research provides a reference for solving the problem of high mortality of A. fangsiao and other mollusks and provides a reference for the future production of some disease-resistant A. fangsiao.

2.
Foods ; 12(2)2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36673332

RESUMO

To overcome the issues in the traditional deacidification processes of peony seed oil (PSO), such as losses of neutral oil and trace nutrients, waste discharge, and high energy consumption, adsorption deacidification was developed. The acid removal capacity of adsorbent-alkali microcrystalline cellulose was evaluated using the isothermal adsorption equilibrium and the pseudo-first-order rate equation. The optimized adsorption deacidification conditions included adsorbent-alkali microcrystalline cellulose at 3%, a heating temperature of 50 °C, and a holding time of 60 min. The physicochemical, bioactive properties, antioxidant capacities, and oxidative stabilities of PSO processed by alkali refining and oil-hexane miscella deacidification were compared under the same operating conditions. Fatty acid content was not significantly different across all three methods. The deacidification rates were 88.29%, 98.11%, and 97.76%, respectively, for adsorption deacidification, alkali refining, and oil-hexane miscella deacidification. Among the three deacidification samples, adsorption deacidification showed the highest retention of tocopherols (92.66%), phytosterols (91.96%), and polyphenols (70.64%). Additionally, the obtained extract preserved about 67.32% of the total antioxidant activity. The oil stability index was increased 1.35 times by adsorption deacidification. Overall, adsorption deacidification can be considered a promising extraction technology in terms of quality as compared to alkali refining and oil-hexane miscella deacidification.

3.
Adv Mater ; : e2210854, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36621966

RESUMO

Atomically two-dimensional (2D) layered ferroelectric semiconductors, in which the polarization switching process occurs within the channel material itself, offer a new material platform that could drive electronic components towards structural simplification and high-density integration. Here, a room-temperature 2D layered ferroelectric semiconductor, bismuth oxychalcogenides (Bi2 O2 Se), is investigated with a thickness down to 7.3 nm (∼12 layers) and piezoelectric coefficient (d33 ) of 4.4 ± 0.1 pm V-1 . The random orientations and electrically-dependent polarization of the dipoles in Bi2 O2 Se are separately uncovered owing to the structural symmetry-breaking at room temperature. Specifically, the interplay between ferroelectricity and semiconducting characteristics of Bi2 O2 Se is explored on device-level operation, revealing the hysteresis behaviors and memory windows formation. Leveraging the ferroelectric polarization originating from Bi2 O2 Se, the fabricated device exhibits "smart" photoresponse tunability and excellent electronic characteristics, e.g., a high on/off current ratio > 104 and a large memory window to the sweeping range of 47% at VGS = ±5 V. These results demonstrate the synergistic combination of ferroelectricity with semiconducting characteristics in Bi2 O2 Se, laying the foundation for integrating sensing, logic and memory functions into a single material system that could overcome the bottlenecks in von Neumann architecture. This article is protected by copyright. All rights reserved.

4.
Addict Behav ; 140: 107607, 2023 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-36652812

RESUMO

It is well established that young adults' drinking is positively associated with sexual activity with new partners. While pharmacologic effects of alcohol (e.g., impaired sexual decision-making) can contribute to sexual activity with new partners, the context in which alcohol is consumed may also be important. We tested the hypothesis that drinking venues such as parties and bars increase the likelihood of sexual activity with new partners beyond any pharmacological effects of drinking quantity. Participants were 427 first-year men (aged 18-19). They reported on their relationship status and sociosexuality (i.e., attitudes/behaviors related to casual sex) at baseline and completed daily diary reports of drinking events (including number of drinks and venue) and sexual activity with new or previous partners over the span of 56 days. Drinking events at parties/bars (but not at home) increased the odds of sexual activity in the next four hours with new partners (but not with previous partners), even after controlling for the number of drinks consumed. Parties/bars are an integral part of facilitating sexual activity with new partners, and have effects independent of pharmacological alcohol effects, which has implications for reducing alcohol-related risky sexual activity. Interventions should not only target alcohol consumption, but where consumption takes place.

5.
Ophthalmic Res ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36689936

RESUMO

INTRODUCTION: This study aimed to compare anatomical outcomes of air and perfluoropropane gas (C3F8) tamponade in pars plana vitrectomy for the treatment of rhegmatogenous retinal detachment (RRD). METHODS: In this retrospective study, data were gathered from 578 patients (578 eyes) with RRD. The follow-up records of all 578 patients that underwent primary vitrectomy for RRD with air or C3F8 were examined and analyzed. Surgical outcomes of the two groups were compared. RESULTS: A total of 342 eyes were treated with air and 236 with C3F8. The mean follow-up period was 37.65 ± 2.33 months. Baseline and preoperative clinical characteristics were similar between groups, but the period to intraocular bubble disappearance (P < 0.0001), intraocular pressure on the first postoperative day (P < 0.0001), number of cases with intraocular pressure > 21mmHg within three days post-surgery (P < 0.0001) and the number with intraocular pressure > 21mmHg during follow-up (P = 0.0002) differed significantly between groups. Primary reattachment rates for air and C3F8 groups were 95.03% and 95.34%, respectively. Clinical characteristics were similar in those with and without successful reattachment, and the frequency of new or unclosed breaks was similar between the two groups. There was no significant difference in two groups according to the presence or absence of inferior retinal breaks and inferior detached quadrants. Univariate and multivariate logistic regression identified no risk factor for surgical failure. CONCLUSIONS: Air showed equivalent effects to C3F8, with a shorter period to intraocular bubble disappearance, less risk of post-operative intraocular hypertension, and less expense.

6.
Ophthalmol Ther ; 12(1): 469-484, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36495394

RESUMO

INTRODUCTION: The maculopathy in highly myopic eyes is complex. Its clinical diagnosis is a huge workload and subjective. To simply and quickly classify pathologic myopia (PM), a deep learning algorithm was developed and assessed to screen myopic maculopathy lesions based on color fundus photographs. METHODS: This study included 10,347 ocular fundus photographs from 7606 participants. Of these photographs, 8210 were used for training and validation, and 2137 for external testing. A deep learning algorithm was trained, validated, and externally tested to screen myopic maculopathy which was classified into four categories: normal or mild tessellated fundus, severe tessellated fundus, early-stage PM, and advanced-stage PM. The area under the precision-recall curve, the area under the receiver operating characteristic curve (AUC), sensitivity, specificity, accuracy, and Cohen's kappa were calculated and compared with those of retina specialists. RESULTS: In the validation data set, the model detected normal or mild tessellated fundus, severe tessellated fundus, early-stage PM, and advanced-stage PM with AUCs of 0.98, 0.95, 0.99, and 1.00, respectively; while in the external-testing data set of 2137 photographs, the model had AUCs of 0.99, 0.96, 0.98, and 1.00, respectively. CONCLUSIONS: We developed a deep learning model for detection and classification of myopic maculopathy based on fundus photographs. Our model achieved high sensitivities, specificities, and reliable Cohen's kappa, compared with those of attending ophthalmologists.

7.
Pathogens ; 11(12)2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36558746

RESUMO

African swine fever virus (ASFV) is a pathogen to cause devastating and economically significant diseases in domestic and feral swine. ASFV mainly infects macrophages and monocytes and regulates its replication process by affecting the content of cytokines in the infected cells. There is a limited understanding of host gene expression and differential profiles before and after ASFV infection in susceptible cells. In this study, RNA-seq technology was used to analyze the transcriptomic change in PAMs infected with ASFV at different time points (0 h, 12 h, 24 h). As a result, a total of 2748, 1570, and 560 genes were enriched in group V12 h vs. MOCK, V24 h vs. MOCK, and V24 h vs. V12 h, respectively. These DEGs (differentially expressed genes) in each group were mainly concentrated in the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways related to innate immunization and inflammation, including the NF-κB signaling pathway, Toll-like receptor signaling pathway, TNF signaling pathway, IL-17 signaling pathway, cytokine-cytokine receptor interaction, and chemokine signaling pathway. Furthermore, the increased levels of IL-1ß, TNF-α, IKKß, CXCL2, and TRAF2 and decreased level of IκBα were validated through the qPCR method. These results suggested that ASFV infection can activate the NF-κB signaling pathway in the early stage. In general, this study provides a theoretical basis for further understanding the pathogenesis and immune escape mechanism of ASFV.

8.
Clean Technol Environ Policy ; : 1-15, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36536780

RESUMO

Low-carbon development of China's power sector is the key to achieving carbon peaking and carbon neutrality goals. Based on the logarithmic mean divisor index (LMDI) model, considering the carbon transfer caused by inter-provincial electricity trading, this paper analyzes the influencing factors of CO2 emissions in the provincial power sector and uses K-means clustering method to divide 30 provinces into four categories to analyze the differences in regional carbon emission characteristics. In addition, by establishing different development scenarios, the carbon emission trends and emission reduction potentials of each cluster under different emission reduction measures from 2020 to 2040 are studied, in order to explore the differentiated emission reduction paths of each cluster. The results show that the contribution of influencing factors shows great differences in different provinces. Trends in CO2 emissions vary widely across scenarios. In the reference scenario, the CO2 emissions of each cluster will continue to increase; in the existing policy scenario, the total power industry will peak at 6.1Gt in 2030; in the advance peak scenario that puts more emphasis on the development of advanced technologies and renewable energy under the clean development model, the carbon emission peak will be brought forward to 2025, and the peak will be reduced to 5.2Gt. Finally, differentiated emission reduction paths and measures are proposed for the future low-carbon development of different cluster power industries, providing theoretical reference for the deployment of provincial-level emission reduction work, which is of great significance to the global green and low-carbon transformation. Supplementary Information: The online version contains supplementary material available at 10.1007/s10098-022-02456-1.

10.
Nano Lett ; 2022 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-36579841

RESUMO

Converting vapor precursors to solid nanostructures via a liquid noble-metal seed is a common vapor deposition principle. However, such a noble-metal-seeded process is excluded from the crystalline halide perovskite synthesis, mainly hindered by the growth mechanism shortness. Herein, powered by a spontaneous exothermic nucleation process (ΔH < 0), the Au-seeded CsPbI3 nanowires (NWs) growth is realized based on a vapor-liquid-solid (VLS) growth mode. It is energetically favored that the Au seeds are reacted with a Pb vapor precursor to form molten Au-Pb droplets at temperatures down to 212 °C, further triggering the low-temperature VLS growth of CsPbI3 NWs. More importantly, this Au-seeded process reduces in-bandgap trap states and consequently avoids Shockley-Read-Hall recombination, contributing to outstanding photodetector performances. Our work extends the powerful Au-seeded VLS growth mode to the emerging halide perovskites, which will facilitate their nanostructures with tailored material properties.

11.
J Orthop Surg (Hong Kong) ; 30(3): 10225536221138985, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36374258

RESUMO

BACKGROUND: Surgical techniques related to soft tissue management play critical roles in optimizing surgical outcomes and patient satisfaction in total knee arthroplasty (TKA). Despite the importance of wound closure and bleeding management approaches, no published guidelines/consensus are available. METHODS: Twelve orthopedic surgeons participated in a modified Delphi panel consisting of 2 parts (each part comprising two rounds) from September-October 2018. Questionnaires were developed based on published evidence and guidelines on surgical techniques/materials. Questionnaires were administered via email (Round 1) or at a face-to-face meeting (subsequent rounds). Panelists ranked their agreement with each statement on a five-point Likert scale. Consensus was achieved if ≥70% of panelists selected 4/5, or 1/2. Statements not reaching consensus in Round 1 were discussed and repeated or modified in Round 2. Statements not reaching consensus in Round 2 were excluded from the final consensus framework. RESULTS: Consensus was reached on 13 goals of wound management. Panelists agreed on 38 challenges and 71 strategies addressing surgical techniques or wound closure materials for each tissue layer, and management strategies for blood loss reduction or deep vein thrombosis prophylaxis in TKA. Statements on closure of capsular and skin layers, wound irrigation, dressings and drains required repeat voting or modification to reach consensus. CONCLUSION: Consensus from Asia-Pacific TKA experts highlights the importance of wound management in optimizing TKA outcomes. The consensus framework provides a basis for future research, guidance to reduce variability in patient outcomes, and can help inform recommendations for wound management in TKA.


Assuntos
Artroplastia do Joelho , Humanos , Técnica Delfos , Artroplastia do Joelho/efeitos adversos , Objetivos , Consenso , Inquéritos e Questionários , Hemorragia
12.
Front Microbiol ; 13: 1019876, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36386633

RESUMO

Foot-and-mouth disease virus (FMDV), Senecavirus A (SVA) and swine vesicular disease virus (SVDV) are members of the family Picornaviridae, which can cause similar symptoms - vesicular lesions in the tissues of the mouth, nose, feet, skin and mucous membrane of animals. Rapid and accurate diagnosis of these viruses allows for control measures to prevent the spread of these diseases. Reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR are traditional and reliable methods for pathogen detection, while their amplification reaction requires a thermocycler. Isothermal amplification methods including loop-mediated isothermal amplification and recombinase polymerase amplification developed in recent years are simple, rapid and do not require specialized equipment, allowing for point of care diagnostics. Luminex technology allows for simultaneous detection of multiple pathogens. CRISPR-Cas diagnostic systems also emerging nucleic acid detection technologies which are very sensitivity and specificity. In this paper, various nucleic acid detection methods aimed at vesicular disease pathogens in swine (including FMDV, SVA and SVDV) are summarized.

13.
Front Oncol ; 12: 898640, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36387114

RESUMO

Background: Endoscopic submucosal dissection (ESD) has been accepted as the standard treatment for the appropriate indication of early gastric cancer (EGC). Determining the risk of lymph node metastasis (LNM) is critical for the following treatment selection after ESD. This study aimed to develop a predictive model to quantify the probability of LNM in EGC to help minimize the invasive procedures. Methods: A total of 952 patients with EGC who underwent radical gastrectomy were retrospectively reviewed. LASSO regression was used to help screen the potential risk factors. Multivariate logistic regression was used to establish a predictive nomogram, which was subjected to discrimination and calibration evaluation, bootstrapping internal validation, and decision curve analysis. Results: Results of multivariate analyses revealed that gender, fecal occult blood test, CEA, CA19-9, histologic differentiation grade, lymphovascular invasion, depth of infiltration, and Ki67 labeling index were independent prognostic factors for LNM. The nomogram had good discriminatory performance, with a concordance index of 0.816 (95% CI 0.781-0.853). The validation dataset yielded a corrected concordance index of 0.805 (95% CI 0.770-0.842). High agreements between ideal curves and calibration curves were observed. Conclusions: The nomogram is clinically useful for predicting LNM after ESD in EGC, which is beneficial to identifying patients who are at low risk for LNM and would benefit from avoiding an unnecessary gastrectomy.

14.
J Clin Transl Hepatol ; 10(6): 1086-1098, 2022 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-36381109

RESUMO

Background and Aims: Hepatic sinusoidal obstruction syndrome (HSOS) is a life-threatening syndrome, and a cause is exposure to pyrrolizidine alkaloid (PA)-containing products. It is well-established that retrorsine (RTS), a representative Pas, insults hepatic sinusoidal endothelial cells and ensues congestion of hepatic sinusoids. However, little known about the impact of Pas on gut microbiota and intestinal barrier and inflammation in HSOS. Methods: Mice were gavaged with or without nonabsorbable antibiotics (ABX), followed by a single dose of RTS. The gut microbiota was examined by 16S rDNA sequencing. Results: ABX pretreatment significantly reversed RTS-induced liver damage. RTS altered gut microbiota composition, increasing Gram-negative bacteria and resulting in a sharp elevation of circulating lipopolysaccharides (LPS) in HSOS mice. Gut decontamination with ABX alleviated RTS-induced intestine inflammation, protected against disruption of the intestinal epithelial barrier and gut vascular barrier (GVB), and suppressed hepatic LPS-NF-κB pathway activation in RTS-induced HSOS. Importantly, the LPS level was positively correlated with MELD score in patients with HSOS. Elevated LPS in patients with HSOS confirmed that Gram-negative bacteria were involved in the pathogenesis of HSOS. Conclusions: RTS, a PA, cooperated with gut dysbiosis to cause intestinal inflammation and gut barrier compromise that increased transport of gut-derived LPS into the liver through the portal vein, which contributed to the pathology of HSOS. Modulating the gut microbiota, protecting the intestinal barrier, and suppressing intestinal inflammation with prebiotics or antibiotics might be a useful pharmacologic intervention in HSOS.

15.
Vaccines (Basel) ; 10(11)2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36366327

RESUMO

Foot-and-mouth disease (FMD) is a serious disease affecting the global graziery industry. Once an epidemic occurs, it can lead to economic and trade stagnation. In recent decades, FMD has been effectively controlled and even successfully eradicated in some countries or regions through mandatory vaccination with inactivated foot-and-mouth disease vaccines. Nevertheless, FMD still occurs in some parts of Africa and Asia. The transmission efficiency of foot-and-mouth disease is high. Both disease countries and disease-free countries should always be prepared to deal with outbreaks of FMD. The development of vaccines has played a key role in this regard. This paper summarizes the development of several promising vaccines including progress and design ideas. It also provides ways to develop a new generation of vaccines for FMDV and other major diseases.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36343901

RESUMO

BACKGROUND & AIMS: Our previous study showed that transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) promotes functional enteric nerve regeneration in denervated mice but not through direct transdifferentiation. Homeostasis of the adult enteric nervous system (ENS) is maintained by enteric neural precursor cells (ENPCs). Whether ENPCs are a source of regenerated nerves in denervated mice remains unknown. METHODS: Genetically engineered mice were used as recipients, and ENPCs were traced during enteric nerve regeneration. The mice were treated with benzalkonium chloride to establish a denervation model and then transplanted with BMSCs 3 days later. After 28 days, the gastric motility and ENS regeneration were analyzed. The interaction between BMSCs and ENPCs in vitro was further assessed. RESULTS: Twenty-eight days after transplantation, gastric motility recovery (gastric emptying capacity, P < .01; gastric contractility, P < .01) and ENS regeneration (neurons, P < .01; glial cells, P < .001) were promoted in BMSC transplantation groups compared with non-transplanted groups in denervated mice. More importantly, we found that ENPCs could differentiate into enteric neurons and glial cells in denervated mice after BMSC transplantation, and the proportion of Nestin+/Ngfr+ cells differentiated into neurons was significantly higher than that of Nestin+ cells. A small number of BMSCs located in the myenteric plexus differentiate into glial cells. In vitro, glial cell-derived neurotrophic factor (GDNF) from BMSCs promotes the migration, proliferation, and differentiation of ENPCs. CONCLUSIONS: In the case of enteric nerve injury, ENPCs can differentiate into enteric neurons and glial cells to promote ENS repair and gastric motility recovery after BMSC transplantation. BMSCs expressing GDNF enhance the migration, proliferation, and differentiation of ENPCs.

17.
Artigo em Inglês | MEDLINE | ID: mdl-36344885

RESUMO

Gearbox is an important part of wind turbine. Diagnosing and forecasting gearbox faults of wind turbines can effectively reduce the costs of operation and maintenance and improve the reliability of gearbox operation. Due to high dimensionality and nonlinearity of system parameters, the paper uses the grey relation analysis to select features related to gearbox oil temperature. Features with a relational degree above 0.7 are selected as input data related to oil temperature, including wind speed, ambient temperature, power, and gearbox shaft temperature. Then, a new extreme learning machine with kernel improved by the whale optimization algorithm is established to forecast gearbox oil temperature. Through the residuals between gearbox oil temperature predicted by the proposed model and monitored by the SCADA, whether the gearbox exists faults can be diagnosed. In the case study, the test data was divided into two groups (the test data with and without faults). In the data test without faults, compared with three other models, the proposed model has the smallest false-negative rate (0.211%) and mean absolute percentage error (2.812%). In the data test with faults, the proposed model can diagnose gearbox faults earlier (160 min in advance) than the other three benchmark models. The results show that the proposed hybrid model performs well in the fault diagnosis and prediction of wind turbine gearbox.

18.
Clin Genet ; 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36346122

RESUMO

Immune checkpoint inhibitors (ICIs) improve overall survival in patients with advanced gastric cancer (GC). However, the molecular characterization of GC in ICIs responders is unclear. A total of 288 advanced GC patients were included in this study. Next-generation sequencing analysis was performed on tumor tissue and paired blood to screen for somatic mutants in 639 tumor-associated genes. We demonstrated that ARID1A, HER2/3/4, KMT2C/2D, LRP1B, PIK3CA, SPTA1, and TP53 mutations were significantly correlated with high tumor mutation burden (TMB) score, as well as HER2 amplification. For HER2 and PIK3CA mutations types, this relationship was statistically significant with age and TP53 mutation status, which was also found in the CDH1 gene. These results were confirmed by sequencing 873 GC cases in the cBioPortal database. PIK3CA mutations appear to be associated with longer survival in elderly population and TP53 mutant subtypes. For the first time, we found that GC patients ≥60 years old or with TP53 mutated type and PIK3CA mutations were associated with higher TMB and better ICI response. Building upon the age and TP53 mutation status, this study suggested a novel stratification approach to GC patients and explored the correlations between genetic somatic mutations and TMB score.

19.
Sensors (Basel) ; 22(19)2022 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-36236518

RESUMO

Ship fire is one of the greatest dangers to ship navigation safety. Nevertheless, typical detection methods have limited detection effectiveness and accuracy due to distance restrictions and ship motion. Although the issue can be addressed by image recognition algorithms based on deep learning, the computational complexity and efficiency for ship detection are tough. This paper proposes a lightweight target identification technique based on the modified YOLOv4-tiny algorithm for the precise and efficient detection of ship fires, taking into account the distinctive characteristics of ship fires and the marine environment. Initially, a multi-scale detection technique is applied to broaden the detection range and integrate deep semantic information, thereby enhancing the feature information of small targets and obscured objects and improving the detection precision. Then, the proposed algorithm employs the SE attention mechanism for inter-channel feature fusion to improve the capability of feature extraction and the precision of ship fire detection. Last but not least, picture transformation and migration learning are added to the small ship fire dataset to accelerate the convergence pace, improve the convergence effect, and reduce dataset dependence. The simulation experiments reveal that the proposed I-YOLOv4-tiny + SE model outperforms the benchmark algorithm in terms of ship fire detection accuracy and detection efficiency and that it satisfies the real-time ship fire warning criteria in demanding maritime environments.


Assuntos
Incêndios , Navios , Algoritmos , Simulação por Computador , Redes Neurais de Computação
20.
Front Pharmacol ; 13: 941182, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36238550

RESUMO

Thiopurines, including thioguanine (TG), 6-mercaptopurine (6-MP), and azathioprine (AZA), are extensively used in clinical practice in children with acute lymphoblastic leukemia (ALL) and inflammatory bowel diseases. However, the common adverse effects caused by myelosuppression and hepatotoxicity limit their application. Metabolizing enzymes such as thiopurine S-methyltransferase (TPMT), nudix hydrolase 15 (NUDT15), inosine triphosphate pyrophosphohydrolase (ITPA), and drug transporters like multidrug resistance-associated protein 4 (MRP4) have been reported to mediate the metabolism and transportation of thiopurine drugs. Hence, the single nucleotide polymorphisms (SNPs) in those genes could theoretically affect the pharmacokinetics and pharmacological effects of these drugs, and might also become one of the determinants of clinical efficacy and adverse effects. Moreover, long-term clinical practices have confirmed that thiopurine-related adverse reactions are associated with the systemic concentrations of their active metabolites. In this review, we mainly summarized the pharmacogenetic studies of thiopurine drugs. We also evaluated the therapeutic drug monitoring (TDM) research studies and focused on those active metabolites, hoping to continuously improve monitoring strategies for thiopurine therapy to maximize therapeutic efficacy and minimize the adverse effects or toxicity. We proposed that tailoring thiopurine dosing based on MRP4, ITPA, NUDT15, and TMPT genotypes, defined as "MINT" panel sequencing strategy, might contribute toward improving the efficacy and safety of thiopurines. Moreover, the DNA-incorporated thioguanine nucleotide (DNA-TG) metabolite level was more suitable for red cell 6-thioguanine nucleotide (6-TGNs) monitoring, which can better predict the efficacy and safety of thiopurines. Integrating the panel "MINT" sequencing strategy with therapeutic "DNA-TG" monitoring would offer a new insight into the precision thiopurine therapy for pediatric acute lymphoblastic leukemia patients.

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