Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 157
Filtrar
1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(6): 1807-1811, 2021 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-34893115

RESUMO

OBJECTIVE: To explore the diagnostic value of bone marrow cell morphology combined with immunohistochemistry in patients with primary bone marrow lymphoma. METHODS: The clinical data of 23 patients with primary bone marrow lymphoma diagnosed in the First Affiliated Hospital of Xi'an Jiaotong University from January 2010 to December 2019 were collected. The characteristics of bone marrow aspiration, bone marrow biopsy and immunohistochemistry results were analyzed retrospectively, and the diagnostic value of bone marrow cell morphology combined with immunohistochemistry in primary bone marrow lymphoma were clarified. RESULTS: Most of primary bone marrow lymphoma was B-cell lymphoma, among which diffuse large B-cell lymphoma was the most common pathological type. Typical lymphoma cells could be found in all the patients. 78.26% of the patients could be diagnosed as lymphoma with pathological type, while 91.30% were diagnosed as lymphoma through combined with the bone marrow immunohistochemistry. CONCLUSION: Bone marrow cell morphology combined with immunohistochemistry shows very important diagnostic value in patients with primary bone marrow lymphoma.

2.
Cancer Lett ; 527: 174-190, 2021 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-34929335

RESUMO

Growing evidence suggests that the bidirectional interactions between cancer cells and their surrounding environment, namely the tumor microenvironment (TME), contribute to cancer progression, metastasis, and resistance to treatment. Intense investigation of the Hippo pathway, which controls multiple central cellular functions in tumorigenesis, was focused on cancer cells. However, the role of the Hippo pathway in modulating tumor-stromal interactions in triple-negative breast cancer remains largely unknown. Therefore, this study focused on revealing the effects of Hippo-YAP/TAZ signaling on the immune microenvironment. Our findings reveal that the activity of the Hippo pathway is associated with worse disease outcomes in TNBC and could increase TAM infiltration through the TAZ/IL-34 axis, leading to an immunosuppressive microenvironment and impairing the treatment efficacy of anti-PD-L1. Thus, the TAZ/IL-34 axis may serve as a novel target for TNBC patients.

3.
Front Psychiatry ; 12: 774192, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34925101

RESUMO

Background: Repetitive transcranial magnetic stimulation (rTMS) has therapeutic effects on craving in methamphetamine (METH) use disorder (MUD). The chronic abuse of METH causes impairments in executive function, and improving executive function reduces relapse and improves treatment outcomes for drug use disorder. The purpose of this study was to determine whether executive function helped predict patients' responses to rTMS treatment. Methods: This study employed intermittent theta burst stimulation (iTBS) rTMS modalities and observed their therapeutic effects on executive function and craving in MUD patients. MUD patients from an isolated Drug Rehabilitation Institute in China were chosen and randomly allocated to the iTBS group and sham-stimulation group. All participants underwent the Behavior Rating Inventory of Executive Function - Adult Version Scale (BRIEF-A) and Visual Analog Scales (VAS) measurements. Sixty-five healthy adults matched to the general condition of MUD patients were also recruited as healthy controls. Findings: Patients with MUD had significantly worse executive function. iTBS groups had better treatment effects on the MUD group than the sham-stimulation group. Further Spearman rank correlation and stepwise multivariate regression analysis revealed that reduction rates of the total score of the BRIEF-A and subscale scores of the inhibition factor and working memory factor in the iTBS group positively correlated with improvements in craving. ROC curve analysis showed that working memory (AUC = 87.4%; 95% CI = 0.220, 0.631) and GEC (AUC = 0.761%; 95% CI = 0.209, 0.659) had predictive power to iTBS therapeutic efficacy. The cutoff values are 13.393 and 59.804, respectively. Conclusions: The iTBS rTMS had a better therapeutic effect on the executive function of patients with MUD, and the improved executive function had the potential to become a predictor for the efficacy of iTBS modality for MUD treatment. Clinical Trial Registration: ClinicalTrials.gov, identifier: ChiCTR2100046954.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34820731

RESUMO

The distribution patterns and health risk assessment of nitrated polycyclic aromatic hydrocarbons (NPAHs), hydroxy polycyclic aromatic hydrocarbons (OH-PAHs), and regular 16 priority polycyclic aromatic hydrocarbons (PAHs) in sediment from the Songhua River in northeastern China were investigated in this research. During dry seasons, concentrations of 16 USEPA priority PAHs, OH-PAHs, and NPAHs were extremely high, with average values of 1220 ± 288, 317 ± 641, 2.54 ± 3.98, and 12.2 ± 22.1 ng/g (dry weight, dw). The dry period level was confirmed to be 4 times greater than the wet period concentration. Modeling with positive matrix factorization (PMF) and estimation of diagnostic isomeric ratios were applied for identifying sources, according to the positive matrix factorization model: vehicle emissions (38.1%), biomass burning (25%), petroleum source (23.4%), and diesel engines source (13.5%) in wet season as well as wood combustion (44.1%), vehicle source (40.2%), coke oven (10.8%), and biomass burning (4.9%) in the dry season. The greatest seasonal variability was attributed to high molecular weight compounds (HMW PAHs). BaP was confirmed to be 81% carcinogenic in this study, which offers convincing proof of the escalating health issues.

5.
Front Endocrinol (Lausanne) ; 12: 740902, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621244

RESUMO

Fatty acid binding protein 4 (FABP4) has been associated with insulin resistance. Gestational diabetes mellitus (GDM) impairs fetal insulin sensitivity. Female newborns are more insulin resistant than male newborns. We sought to evaluate the association between GDM and cord blood FABP4, and explore potential sex dimorphic associations and the roles of sex hormones. This was a nested case-control study in the Shanghai Birth Cohort, including 153 pairs of newborns in GDM vs. euglycemic pregnancies matched by infant sex and gestational age at delivery. Cord plasma FABP4, leptin, total and high-molecular-weight adiponectin, testosterone and estradiol concentrations were measured. Adjusting for maternal and neonatal characteristics, cord plasma FABP4 (Mean ± SD: 27.0 ± 19.6 vs. 18.8 ± 9.6 ng/mL, P=0.045) and estradiol (52.0 ± 28.6 vs. 44.2 ± 26.6, ng/mL, P=0.005) concentrations were higher comparing GDM vs. euglycemic pregnancies in males, but similar in females (all P>0.5). Mediation analyses showed that the positive association between GDM and cord plasma FABP4 in males could be partly mediated by estradiol (P=0.03), but not by testosterone (P=0.72). Cord plasma FABP4 was positively correlated with total adiponectin in females (r=0.17, P=0.053), but the correlation was in the opposite direction in males (r=-0.11, P=0.16) (test for difference in r, P=0.02). Cord plasma FABP4 was not correlated with leptin in both sexes. The study is the first to demonstrate sex-dimorphic associations between GDM and cord plasma FABP4 or estradiol, and between FABP4 and adiponectin in newborns. GDM may affect fetal circulating FABP4 and estradiol levels in males only.

6.
Mol Med Rep ; 24(3)2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34296310

RESUMO

Ferroptosis is critically involved in the pathophysiology of diabetic nephropathy (DN). As a bioactive peptide, salusin­ß is abundantly expressed in the kidneys. However, it is unclear whether salusin­ß participates in the pathologies of diabetic kidney damage by regulating ferroptosis. The present study found that high glucose (HG) treatment upregulated the protein expressions of salusin­ß in a dose­ and time­dependent manner. Genetic knockdown of salusin­ß retarded, whereas overexpression of salusin­ß aggravated, HG­triggered iron overload, antioxidant capability reduction, massive reactive oxygen species production and lipid peroxidation in HK­2 cells. Mechanistically, salusin­ß inactivated nuclear factor erythroid­derived 2­like 2 (Nrf­2) signaling, thus contributing to HG­induced ferroptosis­related changes in HK­2 cells. Notably, the protein expression of salusin­ß was upregulated by ferroptosis activators, such as erastin, RSL3, FIN56 and buthionine sulfoximine. Pretreatment with ferrostatin­1 (a ferroptosis inhibitor) prevented the upregulated protein expression of salusin­ß in HK­2 cells exposed to HG. Taken together, these results suggested that a positive feedback loop between salusin­ß and ferroptosis primes renal tubular cells for injury in diabetes.


Assuntos
Ferroptose/genética , Peptídeos e Proteínas de Sinalização Intercelular/genética , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Transdução de Sinais/efeitos dos fármacos , Butionina Sulfoximina/farmacologia , Carbolinas/farmacologia , Linhagem Celular , Nefropatias Diabéticas/metabolismo , Ferroptose/efeitos dos fármacos , Glucose/toxicidade , Humanos , Peroxidação de Lipídeos/genética , Oximas/farmacologia , Piperazinas/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/genética , Sulfonamidas/farmacologia , Regulação para Cima/efeitos dos fármacos
7.
ACS Omega ; 6(28): 17766-17775, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34308012

RESUMO

The biological reduction of ferrous ethylenediaminetetraacetic acid (EDTA-FeII-NO and EDTA-FeIII) is an important process in the integrated electrobiofilm reduction method, and it has been regarded as a promising alternative method for removing NO x from industrial boiler flue gas. EDTA-FeII-NO and EDTA-FeIII are crucial substrates that should be biologically reduced at a high rate. However, they inhibit the reduction processes of one another when these two substrates are presented together, which might limit further promotion of the integrated method. In this study, an integrated electrobiofilm reduction system with high reduction rates of EDTA-FeII-NO and EDTA-FeIII was developed. The dynamic changes of microbial communities in the electrobiofilms were mainly investigated to analyze the changes during the reduction of these two substrates under different conditions. The results showed that compared to the conventional chemical absorption-biological reduction system, the reduction system exhibited better performance in terms of resistance to substrate shock loading and high microbial diversities. High-throughput sequencing analysis showed that Alicycliphilus, Enterobacteriaceae, and Raoultella were the dominant genera (>25% each) during the process of EDTA-FeII-NO reduction. Chryseobacterium had the ability to endure the shock loading of EDTA-FeIII, and the relative abundance of Chryseobacterium under abnormal operation conditions was up to 30.82%. Ochrobactrum was the main bacteria for reducing nitrate by electrons and the relative abundance still exhibited 16.11% under shock loading. Furthermore, higher microbial diversity and stable reactor operation were achieved when the concentrations of EDTA-FeII-NO and EDTA-FeIII approached the same value (9 mmol·L-1).

8.
Analyst ; 146(14): 4566-4575, 2021 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-34152330

RESUMO

p-Nitrophenol and its derivatives can cause serious harm to the health of mankind and the earth's ecosystem. Therefore, it is necessary to develop a novel and rapid detection technology for p-nitrophenol and its derivative. Herein, excellent water-soluble, large-size and dual-emissive neuron cell-analogous carbon-based probes (NCNPs) have been prepared via a solvothermal approach, using o-phenylenediamine as the only precursor, which exhibit two distinctive fluorescence (FL) peaks at 420 and 555 nm under 345 nm excitation. The NCNPs show a neuron cell-like branched structure, are cross-connected, and are in the range of 10-20 nm in skeleton diameter. Interestingly, their blue-green dual-colour fluorescence is quenched by p-nitrophenol or its derivative due to the specific mechanism of the ππ stacking interactions or internal filtration effect. Accordingly, a simple, rapid, direct and free-label ratiometric FL detection of p-nitrophenol is proposed. An excellent linear relationship shows linear regions over the range of 0.1-50 µM between the ratio of the FL intensity (FL555 nm/FL420 nm) and the concentrations of p-nitrophenol. The detection limit is as low as 43 nM (3σ). Importantly, the NCNP-based probe also shows acceptable repeatability and reproducibility for the detection of p-nitrophenol and its derivatives, and the recovery results for p-nitrophenol in real wastewater samples are favourable.


Assuntos
Pontos Quânticos , Ecossistema , Corantes Fluorescentes , Neurônios , Nitrofenóis , Reprodutibilidade dos Testes
9.
Cancer Manag Res ; 13: 3651-3661, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33981164

RESUMO

Introduction: This study aims at exploring the expression and significance of recombination signal-binding protein for immunoglobulin kappa J region (RBP-Jκ) and C-X-C motif chemokine 11 (CXCL11) in human colon cancer tissues. Methods: The RBP-Jκ and CXCL11 expression levels were assessed by immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) in patients with colon cancer, and their prognostic significance was evaluated. Results: Through analyzing 342 samples of colon cancer patients treated at our institution, increased expression of RBP-Jκ and CXCL11 was found in human colon cancer specimens compared with matched paratumorous normal specimens (P<0.001). A positive correlation was found between RBP-Jκ expression and CXCL11 expression (P<0.001). High RBP-Jκ expression was significantly associated with poorly differentiated tumors (P=0.005), invasion beyond propria muscularis (P=0.025), lymph node metastases (P=0.005), distant metastasis (P<0.001), advanced tumor-node-metastasis (TNM) stage (P=0.004), and a shorter overall survival (P<0.001). An increase in CXCL11 protein expression was associated with poorly differentiated tumors (P=0.015), invasion beyond propria muscularis (P=0.029), lymph node metastases (P=0.031), distant metastasis (P=0.045), advanced TNM stage (P=0.026), and a shorter overall survival of patients (P<0.001). In multivariate Cox regression analysis, RBP-Jκ protein expression (P=0.036), CXCL11 protein expression (P=0.001), differentiation (P<0.001), depth of invasion (P=0.009), distant metastasis (P<0.001), and TNM stage (P<0.001) were independent prognostic indicators of colon cancer. Conclusion: High expression of RBP-Jκ is closely associated with high CXCL11 expression, which represents a risk factor for the poor overall survival of colon cancer patients.

10.
Exp Gerontol ; 151: 111403, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33984448

RESUMO

Renal fibrosis plays a crucial role in the progression of chronic kidney disease and end-stage renal disease. However, because the aetiology of this pathological process is complex and remains unclear, there is still no effective treatment. Cellular senescence and the senescence-associated secretory phenotype (SASP) have been reported to lead to renal fibrosis. This review first discusses the relationships among cellular senescence, the SASP and renal fibrosis. Then, the key role of the SASP in irreversible renal fibrosis, including fibroblast activation and abnormal extracellular matrix accumulation, is discussed, with the results of studies having indicated that inhibiting cellular senescence and the SASP might be a potential preventive and therapeutic strategy for renal fibrosis. Finally, we summarize promising therapeutic strategies revealed by existing research on senescent cells and the SASP, including emerging interventions targeting the SASP, caloric restriction and mimetics, and novel regeneration therapies with stem cells.


Assuntos
Senescência Celular , Nefropatias , Matriz Extracelular , Fibrose , Humanos , Nefropatias/terapia , Fenótipo , Células-Tronco
11.
Inorg Chem ; 60(5): 3156-3164, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33591741

RESUMO

A bifunctional ligand strategy for modification of the functional pores is of great significance in the structural design of metal-organic frameworks (MOFs). Herein, a new 2-fold interpenetrated "pillared-layer" 3D Co-MOF, {[Co(HL)(4,4'-bipy)]·DMF·2H2O}n (1), was successfully synthesized by using two kinds of ligands, imidazolecarboxylic acid and pyridine. The metal-carboxylic layers are pillared by the 4,4'-bipy ligand, displaying a 3D framework with rectangular 3D channels (high BET surface of 190.9 m2 g-1 and maximum aperture of 3.9 Å) that are decorated with abundant uncoordinated N and O atoms. 1 shows good water stability and thermal stability (320 °C). The proper pores and active sites endowed 1 with a selective adsorption of Congo red in aqueous solution. In addition, a high CO2 adsorption capacity and an excellent CO2 chemical conversion were observed.

12.
Hemoglobin ; 45(1): 46-48, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33588687

RESUMO

ß-Thalassemia (ß-thal) is an inherited blood disorder characterized by reduced or absent synthesis of the ß chains of hemoglobin (Hb). Although more than 900 ß-globin gene mutations around the world have been identified, here we report a novel mutation detected in a Chinese subject of Han ethnicity. This allele develops by insertion of one nucleotide (+T) at codon 130 (HBB: c.391insT) in the third exon of the ß-globin gene. The mutation causes a frameshift that leads to a termination codon at codon 139. The identification of the novel mutation will facilitate future diagnosis of ß-thal and will also be useful the genetic counseling and prenatal diagnosis.

13.
Ann Palliat Med ; 10(2): 2008-2017, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33353357

RESUMO

BACKGROUND: To evaluate the characteristics of drug-related problems (DRPs) in cancer pain patients, and to identify the impact of pharmacists' intervention in cancer pain associated DRPs. METHODS: In this investigative, single-arm intervention study, clinical pharmacists identified DRPs in cancer pain patients and provided interventions based on medication information, direct patient-pharmacist interview, and ward rounds with multi-disciplinary team (MDT). Types and causes of DRPs, interventions, acceptance and outcome were sorted based on Pharmaceutical Care Network Europe (PCNE) DRP classification V9.0, which includes 3 primary domains for problems, 9 for causes, 5 for interventions, 3 for acceptance, and 4 for DRPs status. RESULTS: Totally, 42 cancer pain patients were enrolled, and 47 DRPs in 33 (78.6%) patients were identified by clinical pharmacists. The major type of DRPs was treatment effectiveness (30; 63.8%) and treatment safety (17; 36.2%). For the "treatment effectiveness" category, the "effect of drug treatment not optimal" was dominant category (27/30; 90%). A total of 66 DRP causes were identified, and most of DRPs were caused by "drug selection" (27; 40.9%) and "dose selection" (16; 24.2%). Within the "drug selection" category, "no or incomplete drug treatment in spite of existing indication" was dominant category (25/27; 92.6%). According to DRPs, 159 interventions were provided by clinical pharmacists and 99.4% of interventions were accepted by prescribers or patients. Finally, 44 (93.6%) DRPs were solved. CONCLUSIONS: In cancer pain patients, insufficient pain control mainly caused by inappropriate selection and dosage of analgesics. Clinical pharmacists' interventions dramatically ameliorate these problems and bring about positive effects in cancer pain pharmacotherapy.


Assuntos
Dor do Câncer , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Neoplasias , Preparações Farmacêuticas , Dor do Câncer/tratamento farmacológico , Europa (Continente) , Humanos , Neoplasias/complicações
14.
Analyst ; 146(3): 874-881, 2021 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-33241796

RESUMO

Recently, the development of a novel fluorescent (FL) nanoprobe for ratiometric detection of antibiotics in real-world samples has received more and more attention. In this article, the distinctive optical properties of deep-ultraviolet emission, a narrowed full width at half maximum (∼20 nm) and excitation-independent emission of a carbonized nanoprobe (CNP) were easily prepared by an environmentally friendly approach of solvothermal treatment using melamine as the precursor and H2O as the solvent. The obtained CNP can be further utilized as an efficient ratiometric FL nanoprobe for enrofloxacin (EFC) and feroxacin (FXC) detection based on the fact that the FL quenching of the CNP was accompanied by an FL increase with EFC/FXC based on the inner filter effect (IFE). Under the optimal conditions, excellent linear relationships existed between the relative FL intensity (FL290 nm/FL412 nm, CNP for FL290 nm and antibiotics for FL412 nm) and the concentrations of FXC and EFC in the range of 0.05-500.0 µM and 0.05-200.0 µM, with limits of detection of 21.74 and 22.43 nM (3σ/k), respectively. With the proposed ratiometric FL sensor, FXC and EFC in milk and serum samples can be rapidly and selectively analyzed without tedious pretreatment processes for real-world samples.


Assuntos
Corantes Fluorescentes , Enrofloxacina
15.
Front Neurol ; 12: 747551, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34975715

RESUMO

Aims: Although prognostic importance of ultraearly hematoma growth (uHG) in acute, non-traumatic intracerebral hemorrhage (ICH) has been established for early outcomes, longer-term clinical outcomes are lacking. We aimed to determine the association of uHG with early and 1-year clinical outcomes after acute ICH in a larger and broader range of patients. Methods: We studied 589 patients with acute (<6 h) spontaneous ICH. uHG was defined as baseline ICH volume/onset-to-imaging time (OIT) (ml/h). Multivariable logistic regression analyses were performed to determine the association of uHG with in-hospital mortality, 90-day, and 1-year poor outcome [3 ≤ modified Rankin Scale (mRS)] after ICH. Results: The median speed of uHG was 4.8 ml/h. uHG > 9.3 ml/h was independently related to in-hospital mortality [odds ratio (OR) 2.81, 95% CI 1.52-5.23], 90-day poor outcome (OR 3.34, 95% CI 1.87-5.95), and 1-year poor outcome (OR 3.59, 95% CI 2.01-6.40) after ICH. The sensitivity of uHG > 9.3 ml/h in the prediction of in-hospital mortality, 90-day poor outcome, and 1-year poor outcome was 68.8, 48.0, and 51.1%, respectively. Conclusions: Ultraearly hematoma growth was a useful predictor of in-hospital mortality, 90-day, and 1-year poor outcome after acute ICH. The combination of both uHG and baseline ICH volume could allow better selection of patients with ICH at high risk of poorest clinical outcomes for future clinical trials to improve early- and long-term clinical outcomes.

16.
Front Endocrinol (Lausanne) ; 11: 567955, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33117283

RESUMO

Fetuin-A is a multifunctional glycoprotein that has been implicated in insulin resistance and bone metabolism. We assessed whether fetuin-A is associated with poor or excessive fetal growth. In the Shanghai Birth Cohort, we conducted a nested case-control study of 60 trios of small-for-gestational-age (SGA, birth weight <10th percentile), optimal-for-gestational-age (OGA, 25-75th, the reference) and large-for-gestational-age (LGA, >90th percentile) infants matched by sex and gestational age. Cord plasma concentrations of fetuin-A and fetal growth factors [insulin, proinsulin, insulin-like growth factor (IGF)-I and IGF-II] were measured. Cord plasma fetuin-A concentrations were higher in SGA (809.4 ± 306.9 µg/ml, P = 0.026) and LGA (924.2 ± 375.9 µg/ml, P < 0.001) relative to OGA (680.7 ± 262.1 µg/ml) newborns, and were not correlated to insulin, proinsulin, IGF-I and IGF-II (all P > 0.2). Higher fetuin-A concentrations were associated with increased risks of SGA [OR = 1.67 (1.08-2.58) per SD increment, P = 0.024] and LGA [OR = 2.36 (1.53-3.66), P < 0.001]. Adjusting for maternal and neonatal characteristics and fetal growth factors, the elevated risk changed little for LGA [adjusted OR = 2.28 (1.29-4.01), P = 0.005], but became non-significant for SGA (P = 0.202). Our study is the first to demonstrate that fetuin-A may be involved in excessive fetal growth. This association is independent of fetal growth factors.


Assuntos
Peso ao Nascer/fisiologia , Desenvolvimento Fetal/fisiologia , Idade Gestacional , Recém-Nascido Pequeno para a Idade Gestacional/sangue , alfa-2-Glicoproteína-HS/metabolismo , Biomarcadores/sangue , Estudos de Casos e Controles , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Recém-Nascido , Gravidez
17.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(5): 1486-1490, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33067942

RESUMO

OBJECTIVE: To investigate the consistency between pathologic immunohistochemistry assay and flow immunotyping of patients with lymphoma cell leukemia. METHODS: The immunohistochemistry and flow immunotyping data of 31 patients with non-hodgkin lymphoma admitted in our hospital from January 2012 to December 2018 were analyzed retrospectively. The pathologic immunohistochemical expression of lymphatic cells was compared with that of flow immunotyping types, and the change of expression rate of various antigens in the progression of lymphomas into leukemia stage was studied. The characteristics of immune typing and pathologic immunohistochemistry of lymphoblastic leukemia, and their diagnostic value in lymphoblastic leukemia was observed. RESULTS: All patients with lymphoma reached the leukemia stage. The results of flow immunotyping were basically consistent with the results of pathological immunohistochemistry. The pathological immunohistochemistry showed that CD5, CD3, CD99, CD7, CD34, CD43, etc. mainly expressed in the patients with T lymphocyte lymphoma leukemia, of which CD5 showed the highest expression rate and its positive rate was 100%. The flow immunotyping showed that CD7, CD3, CD2, CD5, CD11b, CD34 and HLA-DR was mainly expressed in the patients with T lymphocyte lymphoma leukemia, of which CD7 was the most sensitive and its positive rate was 100 %. Although the antigens expressed in the pathologic immunohistochemistry and flow immunotyping were basically consistent, there were differences in the expression rates of various antigens, CD20, CD79a, BCL-2, CD10, and the Hodgkin's lymphoma cell antigen PAX5 derived from B cells them mainly expressed in BLL patients assay by pathological immunohistochemistry, among the expression rate of CD20 was 100%, which was higher than other antigen expression rate. CD19, CD20, CD22, CD79a, skappa, and early antigen HLA-DR mainly expressed in BLL patients assayed by flow immunotyping, among them CD19 showed a positive rate of 94.7%. The results of immunohistochemistry and flow immunotyping were compared, it was found that 42% of the patients were accompanied by CD20 expression loss, and the survival period of these patients was significantly reduced. CONCLUSION: 25% of patients with T-lymphoma leukemia are accompanied by CD3 expression loss, and 42% of patients with B-cell lymphoma leukemia are accompanied by CD20 expression loss. There is no significant change in survival of T-cell lymphoma leukemia patients with CD3 expression loss, however, the survival period of B-cell lymphoma leukemia patients with CD20 expression loss is significantly shortened.


Assuntos
Leucemia Linfocítica Crônica de Células B , Linfoma , Antígenos HLA-DR , Humanos , Imunofenotipagem , Estudos Retrospectivos
18.
J Reprod Immunol ; 142: 103208, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33002799

RESUMO

Immune checkpoint molecules may play a crucial role in safeguarding pregnancy by regulating immune responses at the maternal-fetal interface. In this study, we aim to investigate the expression of PD-1, GITR, HLA-G, and CTLA-4 on T cell subsets in peripheral blood (PB), retroplacental blood (RPB), and cord blood (CB) in normal pregnancy (NP), preeclampsia (PE) and gestational diabetes mellitus (GDM). PB, RPB, and CB were collected immediately after delivery, and the expression of PD-1, GITR, HLA-G, and CTLA-4 on T cell subsets were measured by flow cytometric analysis. The proportions of Tregs in PB, RPB, and CB from NP were significantly higher than those of PE and GDM (P < 0.01, respectively). PD-1+ and GITR+ T cell subsets (CD3+, CD4+, and CD8+ T cells, and Tregs) in PB, as well as PD-1+ T cell subsets in RPB from NP, were significantly higher than those of PE and GDM (P < 0.01, respectively). In NP, PE, and GDM, the proportion of PD-1+ Tregs was significantly decreased in CB as compared to those of PB and RPB (P < 0.05, respectively) and the proportion of GITR+ Tregs was significantly higher in PB as compared to those of CB and RPB (P < 0.01, respectively). The proportion of HLA-G+ Tregs in PB was significantly lower than those of CB and RPB (P < 0.01, respectively). In conclusion, decreased PD-1+ and GITR+ T cell subsets and decreased proportion of Tregs in PB and RPB may play a role in chronic inflammatory immune activation of effector T cells in PE and GDM.


Assuntos
Diabetes Gestacional/imunologia , Proteínas de Checkpoint Imunológico/metabolismo , Pré-Eclâmpsia/imunologia , Subpopulações de Linfócitos T/imunologia , Adulto , Diabetes Gestacional/sangue , Feminino , Sangue Fetal/citologia , Sangue Fetal/imunologia , Feto/imunologia , Humanos , Masculino , Troca Materno-Fetal/imunologia , Pré-Eclâmpsia/sangue , Gravidez , Estudos Prospectivos , Subpopulações de Linfócitos T/metabolismo
19.
J Cell Mol Med ; 24(21): 12873-12878, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33000557

RESUMO

Gliomas are the most common primary brain tumours, and glioblastomas (GBMs) are subgrouped into four distinct molecular subtypes. This study aimed to identify the potential gene related to glioma progression. Weighted gene co-expression network analysis (WGCNA) was used to explore the related gene. Correlation, ROC, survival and Cox regression analyses were performed. Blue module was strongly associated with WHO grade (r = .65, P = 1e-19). GNG5 in gliomas was overexpressed compared with normal samples and associated with clinicopathologic characteristics. GNG5 was frequent in Mesenchymal subtype and lowly expressed in Proneural subtype of GBMs. Survival and Cox regression analyses showed that glioma patients with GNG5 overexpression had shorter survival time, and GNG5 was an independent prognostic indicator of overall survival. Overall, GNG5 expression is closely associated with clinicopathologic characteristics and is an independent prognostic indicator for glioma patients, as well as a promising subtype-associated biomarker in molecular classification of gliomas.


Assuntos
Neoplasias Encefálicas/genética , Subunidades gama da Proteína de Ligação ao GTP/metabolismo , Glioma/genética , Neoplasias Encefálicas/patologia , Subunidades gama da Proteína de Ligação ao GTP/genética , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes , Glioma/patologia , Humanos , Prognóstico
20.
Artigo em Inglês | MEDLINE | ID: mdl-32973071

RESUMO

INTRODUCTION: Gestational diabetes (GD) is associated with impaired insulin sensitivity in newborns. Adiponectin and retinol-binding protein 4 (RBP-4) are involved in regulating insulin sensitivity. Females are more likely to develop diabetes at young ages than males. We tested the hypothesis that GD may affect RBP-4 and adiponectin levels in early life, and there may be sex-dimorphic associations. RESEARCH DESIGN AND METHODS: In a nested case-control study of 153 matched pairs of neonates of mothers with GD and euglycemic pregnancies in the Shanghai Birth Cohort, we evaluated cord plasma leptin, high molecular weight (HMW) and total adiponectin and RBP-4 concentrations. RESULTS: Comparing GD versus euglycemic pregnancies adjusted for maternal and neonatal characteristics in female newborns, cord plasma total adiponectin (mean±SD: 30.8±14.3 vs 37.1±16.1 µg/mL, p=0.048) and HMW adiponectin (14.6±7.7 vs 19.3±8.3 µg/mL, p=0.004) concentrations were lower, while RBP-4 concentrations were higher (21.7±5.4 vs 20.0±4.8 µg/mL, p=0.007). In contrast, there were no differences in male newborns (all p>0.2). RBP-4 concentrations were higher in female versus male newborns (21.7±5.4 vs 18.8±4.5 µg/mL, p<0.001) in GD pregnancies only. HMW adiponectin concentrations were significantly higher in female versus male newborns in euglycemic pregnancies only (19.3±8.3 vs 16.1±7.4 µg/mL, p=0.014). CONCLUSIONS: GD was associated with lower cord plasma HMW adiponectin and higher RBP-4 concentrations in female newborns only. The study is the first to reveal a sex-dimorphic early life impact of GD on metabolic health biomarkers in the offspring. GD may alter the normal presence (HMW adiponectin) or absence (RBP-4) of sex dimorphism in some insulin sensitivity regulation-relevant adipokines in early life.


Assuntos
Adiponectina , Diabetes Gestacional , Estudos de Casos e Controles , China , Feminino , Sangue Fetal , Humanos , Recém-Nascido , Masculino , Gravidez , Caracteres Sexuais
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...