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1.
Sci Rep ; 10(1): 3173, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32081887

RESUMO

The Hippo pathway is a central regulator of organ size and a key tumor suppressor via coordinating cell proliferation and death. Initially discovered in Drosophila, the Hippo pathway has been implicated as an evolutionarily conserved pathway in mammals; however, how this pathway was evolved to be functional from its origin is still largely unknown. In this study, we traced the Hippo pathway in premetazoan species, characterized the intrinsic functions of its ancestor components, and unveiled the evolutionary history of this key signaling pathway from its unicellular origin. In addition, we elucidated the paralogous gene history for the mammalian Hippo pathway components and characterized their cancer-derived somatic mutations from an evolutionary perspective. Taken together, our findings not only traced the conserved function of the Hippo pathway to its unicellular ancestor components, but also provided novel evolutionary insights into the Hippo pathway organization and oncogenic alteration.

2.
J Transl Med ; 18(1): 67, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32046766

RESUMO

BACKGROUND: Growing evidence has suggested that immune-related genes play crucial roles in the development and progression of hepatocellular carcinoma (HCC). Nevertheless, the utility of immune-related genes for evaluating the prognosis of HCC patients are still lacking. The study aimed to explore gene signatures and prognostic values of immune-related genes in HCC. METHODS: We comprehensively integrated gene expression data acquired from 374 HCC and 50 normal tissues in The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) analysis and univariate Cox regression analysis were performed to identify DEGs that related to overall survival. An immune prognostic model was constructed using the Lasso and multivariate Cox regression analyses. Furthermore, Cox regression analysis was applied to identify independent prognostic factors in HCC. The correlation analysis between immune-related signature and immune cells infiltration were also investigated. Finally, the signature was validated in an external independent dataset. RESULTS: A total of 329 differentially expressed immune-related genes were detected. 64 immune-related genes were identified to be markedly related to overall survival in HCC patients using univariate Cox regression analysis. Then we established a TF-mediated network for exploring the regulatory mechanisms of these genes. Lasso and multivariate Cox regression analyses were applied to construct the immune-based prognostic model, which consisted of nine immune-related genes. Further analysis indicated that this immune-related prognostic model could be an independent prognostic indicator after adjusting to other clinical factors. The relationships between the risk score model and immune cell infiltration suggested that the nine-gene signature could reflect the status of tumor immune microenvironment. The prognostic value of this nine-gene prognostic model was further successfully validated in an independent database. CONCLUSIONS: Together, our study screened potential prognostic immune-related genes and established a novel immune-based prognostic model of HCC, which not only provides new potential prognostic biomarkers and therapeutic targets, but also deepens our understanding of tumor immune microenvironment status and lays a theoretical foundation for immunotherapy.

3.
Water Environ Res ; 92(2): 302-309, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31560145

RESUMO

A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons. In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin. The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 . The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 . Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges. Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution. When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively. The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants. PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment. The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 . The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.


Assuntos
Caulim , Purificação da Água , Floculação , Glucanos , Água
4.
EMBO J ; 39(1): e102406, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31782549

RESUMO

The Hippo pathway, which plays a critical role in organ size control and cancer, features numerous WW domain-based protein-protein interactions. However, ~100 WW domains and 2,000 PY motif-containing peptide ligands are found in the human proteome, raising a "WW-PY" binding specificity issue in the Hippo pathway. In this study, we have established the WW domain binding specificity for Hippo pathway components and uncovered a unique amino acid sequence required for it. By using this criterion, we have identified a WW domain-containing protein, STXBP4, as a negative regulator of YAP. Mechanistically, STXBP4 assembles a protein complex comprising α-catenin and a group of Hippo PY motif-containing components/regulators to inhibit YAP, a process that is regulated by actin cytoskeleton tension. Interestingly, STXBP4 is a potential tumor suppressor for human kidney cancer, whose downregulation is correlated with YAP activation in clear cell renal cell carcinoma. Taken together, our study not only elucidates the WW domain binding specificity for the Hippo pathway, but also reveals STXBP4 as a player in actin cytoskeleton tension-mediated Hippo pathway regulation.

5.
Huan Jing Ke Xue ; 40(11): 5048-5056, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854573

RESUMO

In this paper, the low C/N ratio of domestic sewage was studied, and mature phosphorus removal granules were inoculated to investigate the effect of solid retention time(SRT)on phosphorus removal and nitrification granular sludge at middle-low temperatures (14-21℃). The test showed that at room temperature (20℃±1℃), the enrichment of AOB in the phosphorus removal granules can be achieved with 30 d SRT and 5 L·(h·L)-1 aeration intensity, while the NAR was over 90%. When the temperature was lowered to 15℃ and the SRT was 40 d, the phosphorus removal performance deteriorated, and the granule structure became loose with the formation of filamentous bacteria. Relatively sufficient oxygen destabilized nitrosation and the NAR dropped by 22.4%. NOB does not have the ability to quickly adapt to environmental changes. The 12 d anaerobic starvation and sludge removal strategy weakened the relative activity of NOB and quickly restored the performance of phosphorus removal and nitrosation. Batch experiments showed that the temperature dropped from 20℃ to 15℃, and the PAOs still maintained a high oxygen utilization rate, but the SOUR of the AOB decreased by 18%. At this time, the temperature, not the dissolved oxygen concentration, restricted the ammonia oxidation ability. Controlling the sludge age to 30 d, while reducing the aeration intensity to 4 L·(h·L)-1, achieved stable operation of phosphorus removal and nitrosation at a low temperature (15℃±1℃).

6.
Pathogens ; 8(4)2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31635289

RESUMO

The immune competence of an individual is a major determinant of morbidity in West Nile virus (WNV)-infection. Previously, we showed that immunocompetent New Zealand White rabbits (NZWRs; Oryctolagus cuniculus) are phenotypically resistant to WNV-induced disease, thus presenting a suitable model for study of virus-control mechanisms. The current study used corticosteroid-treated NZWRs to model acute "stress"-related immunosuppression. Maximal effects on immune parameters were observed on day 3 post dexamethasone-treatment (pdt). However, contrary to our hypothesis, intradermal WNV challenge at this time pdt produced significantly lower viremia 1 day post-infection (dpi) compared to untreated controls, suggestive of changes to antiviral control mechanisms. To examine this further, RNAseq was performed on RNA extracted from draining lymph node-the first site of virus replication and immune detection. Unaffected by dexamethasone-treatment, an early antiviral response, primarily via interferon (IFN)-I, and induction of a range of known and novel IFN-stimulated genes, was observed. However, treatment was associated with expression of a different repertoire of IFN-α-21-like and IFN-ω-1-like subtypes on 1 dpi, which may have driven the different chemokine response on 3 dpi. Ongoing expression of Toll-like receptor-3 and transmembrane protein-173/STING likely contributed to signaling of the treatment-independent IFN-I response. Two novel genes (putative HERC6 and IFIT1B genes), and the SLC16A5 gene were also highlighted as important component of the transcriptomic response. Therefore, the current study shows that rabbits are capable of restricting WNV replication and dissemination by known and novel robust antiviral mechanisms despite environmental challenges such as stress.

7.
Environ Res ; 178: 108686, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31476683

RESUMO

Rainfall is one of the most fundamental components of the water cycle and is one of the fundamental inputs of hydrological models. A well-designed network can not only depict the regional precipitation characteristics, but also economically yield maximum needed rainfall information. In regions where either there is limited data or data is not available, it is a common challenge to add stations. The entropy theory-based information transfer model and geostatistical interpolation techniques are two solutions to meet the challenge. In this study, we used a representative rain gauge network to do the network design. Two models, based on information transfer and data transfer, were compared for network design. Other rain gauges in the study area were used as reference ("true values") for assessing the model. Results showed that the information transfer model estimated transinformation between station pairs better than did the data transfer model. Different representative gauges were evaluated separately by the directional information transfer index (DIT). The candidate gauges selected with least information redundancy were similar for both information transfer and data transfer models. Though both models captured some least information-redundant areas, other areas may be bypassed because of model errors or estimation errors.

8.
Int J Parasitol Parasites Wildl ; 9: 317-321, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31338292

RESUMO

Wild rats (Rattus spp.) carry many zoonotic pathogens including Cryptosporidium. Due to the close proximity of rats to humans in urban environments, the potential for disease transmission is high. Cryptosporidium is a protozoan parasite which when ingested causes serious human illness. Despite its importance, genetic characterization of Cryptosporidium in wild rats in the Hainan province of China has not been performed. In this study, we analyzed the occurrence and genetics of Cryptosporidium in wild rats from Hainan, China. From December 2017 to October 2018, 150 wild rats were captured and fresh fecal material was collected from intestinal sections. Rat species were identified by PCR-based amplification and analysis of the vertebrate cytochrome b (cytb) gene. Cryptosporidium was examined by PCR amplification of the partial small subunit of ribosomal DNA (SSU rDNA). C. viatorum were subtyped by PCR analysis of the gp60 gene. A total of four rat species were identified including Asian house rats (Rattus tanezumi) (n = 46), brown rats (Rattus norvegicus) (n = 56), Edward's long-tailed rats (Leopoldamys edwardsi) (n = 38) and muridae (Niviventer fulvescens) (n = 10), with Cryptosporidium positive rates of 73.9%, 28.6%, 55.3% and 40.0%, respectively (average infection rate: 50.0%, 75/150. Sequence analysis confirmed the presence of four Cryptosporidium species and two genotypes including C. viatorum (n = 11); C. occultus (n = 2); C. muris (n = 1); and C. erinacei (n = 1); rat genotypes III (n = 13) and IV (n = 47). Three novel subtypes of C. viatorum were identified in 6 of the 11 infected Edward's long-tailed rats: XVcA2G1a (n = 4), XVcA2G1b (n = 1) and XVdA3 (n = 1). The identification of human pathogenic C. viatorum and zoonotic C. occultus, C. muris and C. erinacei, suggested that wild rats infected with Cryptosporidium pose a threat to human health. Taken together, these findings highlight the need to control the rat population in Hainan, China. The need to improve the public awareness of the risk of disease transmission from wild rats to humans is also highlighted.

9.
J Am Chem Soc ; 141(32): 12493-12497, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31357865

RESUMO

A novel strategy for the synthesis of main-chain polymers through radical ring-closing/ring-opening cascade polymerization is reported. Efficient radical cyclopolymerization was achieved through systematic optimization of the electronic properties of 1,6-diene structures. Fusing 1,6-diene with allylic sulfide or allylic sulfone motifs enabled a ring-closing/ring-opening cascade reaction that provides a strong driving force for the ring-opening polymerization of large macrocyclic monomers. The ability of 1,6-diene-fused allylic sulfone to undergo efficient SO2 extrusion generated a propagating alkyl radical capable of reversible deactivation. This strategy provides a general platform for the synthesis of polymers incorporating complex main-chain structures and degradable functionalities.

10.
J Cell Biochem ; 120(8): 12412-12421, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30825222

RESUMO

Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. Accumulating investigations have identified the aberrant expression of miRNAs (microRNAs) in UM, such as miR-181, miR-20a, miR-144, miR-146a. The purpose of this study is to investigate the biological function of miR-224-5p in UM. The expression of miR-224-5p, PIK3R3, and AKT3 in 30 tumor tissues and paired adjacent noncancerous tissues were analyzed using Western blot analysis and quantitative real-time polymerase chain reaction (qRT-PCR) assays. Cell proliferation assay, transwell assay, and wound healing assay were used to measure the effects of miR-224-5p on the motility of UM in vitro. Western blot analysis and luciferase assays were used to detect the expression of PIK3R3 and AKT3 as miR-224-5p downstream targets. The results of Western blot analysis and qRT-PCR assays indicated that the expression of miR-224-5p was lower in UM tissues compared to normal tissue, while the expression of PIK3R3 and AKT3 were simultaneously increased. Upregulation of miR-224-5p significantly inhibited capacities of proliferation, invasion, and migration of OCM-1A cells and decreased expression of PIK3R3 and AKT3. Luciferase assay demonstrated PIK3R3 and AKT3 as downstream targets of miR-224-5p. Moreover, upregulating PIK3R3 and AKT3 restrained miR-224-5p-induced inhibition of the motility of OCM-1A cells. Thus, our study proved that miR-224-5p was involved in proliferation, invasion, and migration of UM cells via regulation the expression of PIK3R3 and AKT3. And the results also established a miR-224-5p/PIK3R3/PI3K/AKT axis in the regulation of UM progression, providing an experimental basis for further exploring the miR-224-5p as a therapeutic and diagnosis target for patients with UM.

11.
Org Lett ; 21(6): 1930-1934, 2019 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-30835486

RESUMO

Efficient asymmetric alkylation of ß,γ-unsaturated carboxylic acids without prior functionalization is enabled by chiral lithium amides. Enantioselectivity is imparted by a putative mixed lithium amide-enediolate aggregate that acts a traceless auxiliary formed in situ, allowing for a direct asymmetric alkylation and a simple recovery of the chiral reagent.


Assuntos
Amidas/química , Ácidos Carboxílicos/química , Lítio/química , Alquilação , Indicadores e Reagentes , Estrutura Molecular , Estereoisomerismo
12.
Mol Cell Oncol ; 6(1): 1558683, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30788423

RESUMO

The Hippo pathway, a signaling pathway highly conserved across species, plays a crucial role in organ size control and cancer suppression. Our recent study shows that phosphatidic acid can regulate the Hippo pathway through a physical lipid-protein interaction, providing additional insights into the Hippo-related tissue homeostasis and cancer development.

13.
Nat Commun ; 10(1): 228, 2019 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-30651536

RESUMO

Single-molecule cytoplasmic dynein function is well understood, but there are major gaps in mechanistic understanding of cellular dynein regulation. We reported a mode of dynein regulation, force adaptation, where lipid droplets adapt to opposition to motion by increasing the duration and magnitude of force production, and found LIS1 and NudEL to be essential. Adaptation reflects increasing NudEL-LIS1 utilization; here, we hypothesize that such increasing utilization reflects CDK5-mediated NudEL phosphorylation, which increases the dynein-NudEL interaction, and makes force adaptation possible. We report that CDK5, 14-3-3ε, and CDK5 cofactor KIAA0528 together promote NudEL phosphorylation and are essential for force adaptation. By studying the process in COS-1 cells lacking Tau, we avoid confounding neuronal effects of CDK5 on microtubules. Finally, we extend this in vivo regulatory pathway to lysosomes and mitochondria. Ultimately, we show that dynein force adaptation can control the severity of lysosomal tug-of-wars among other intracellular transport functions involving high force.


Assuntos
Proteínas 14-3-3/metabolismo , Quinase 5 Dependente de Ciclina/metabolismo , Dineínas do Citoplasma/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , 1-Alquil-2-acetilglicerofosfocolina Esterase/genética , 1-Alquil-2-acetilglicerofosfocolina Esterase/metabolismo , Proteínas 14-3-3/genética , Animais , Fenômenos Biomecânicos , Células COS , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Quinase 5 Dependente de Ciclina/genética , Gotículas Lipídicas/metabolismo , Lisossomos/metabolismo , Proteínas Associadas aos Microtúbulos/genética , Microtúbulos/metabolismo , Mitocôndrias/metabolismo , RNA Interferente Pequeno/metabolismo
14.
Artigo em Inglês | MEDLINE | ID: mdl-30533797

RESUMO

Torque teno virus (TTV) has been reported in a wide range of mammals. In this study, we sequenced and analyzed the complete genome of a genetic variant of a rodent TTV, RoTTV3-HMU1 (Hainan Medical University 1). The virus was found in a rat (Rattus norvegicus) in a residential area of Hainan Island, China.

15.
Micromachines (Basel) ; 9(7)2018 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-30424277

RESUMO

Monitoring the working condition of hydraulic equipment is significance in industrial fields. The abnormal wear of the hydraulic system can be revealed by detecting the variety and size of micro metal debris in the hydraulic oil. We thus present the design and implementation of a micro detection system of hydraulic oil metal debris based on inductor capacitor (LC) resonant circuit in this paper. By changing the resonant frequency of the micro fluidic chip, we can detect the metal debris of hydraulic oil and analyze the sensitivity of the micro fluidic chip at different resonant frequencies. We then obtained the most suitable resonant frequency. The chip would generate a positive resistance pulse when the iron particles pass through the detection area and the sensitivity of the chip decreased with resonant frequency. The chip would generate a negative resistance pulse when the copper particles pass through the detection area and the sensitivity of the chip increased with resonant frequency. The experimental results show that the change of resonant frequency has a great effect on the copper particles and little on the iron particles. Thus, a relatively big resonant frequency can be selected for chip designing and testing. In practice, we can choose a relatively big resonant frequency in this micro fluidic chip designing. The resonant micro fluidic chip is capable of detecting 20⁻30 µm iron particles and 70⁻80 µm copper particles at 0.9 MHz resonant frequency.

17.
Mol Cell ; 72(2): 328-340.e8, 2018 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-30293781

RESUMO

The Hippo pathway plays a crucial role in organ size control and tumor suppression, but its precise regulation is not fully understood. In this study, we discovered that phosphatidic acid (PA)-related lipid signaling is a key regulator of the Hippo pathway. Supplementing PA in various Hippo-activating conditions activates YAP. This PA-related lipid signaling is involved in Rho-mediated YAP activation. Mechanistically, PA directly interacts with Hippo components LATS and NF2 to disrupt LATS-MOB1 complex formation and NF2-mediated LATS membrane translocation and activation, respectively. Inhibition of phospholipase D (PLD)-dependent PA production suppresses YAP oncogenic activities. PLD1 is highly expressed in breast cancer and positively correlates with YAP activation, suggesting their pathological relevance in breast cancer development. Taken together, our study not only reveals a role of PLD-PA lipid signaling in regulating the Hippo pathway but also indicates that the PLD-PA-YAP axis is a potential therapeutic target for cancer treatment.


Assuntos
Metabolismo dos Lipídeos/fisiologia , Ácidos Fosfatídicos/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais/fisiologia , Sequência de Aminoácidos , Animais , Neoplasias da Mama/metabolismo , Linhagem Celular , Linhagem Celular Tumoral , Feminino , Células HEK293 , Humanos , Estimulador Tireóideo de Ação Prolongada/metabolismo , Camundongos , Camundongos Nus , Neurofibromina 2/metabolismo , Proteínas Nucleares/metabolismo , Fosfolipase D/metabolismo , Fosfoproteínas/metabolismo
18.
Nat Cell Biol ; 20(11): 1303-1314, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30361697

RESUMO

Epithelial integrity is maintained by the cytoskeleton and through cell adhesion. However, it is not yet known how a deregulated cytoskeleton is associated with cancer. We identified cancer-related regulator of actin dynamics (CRAD) as frequently mutated or transcriptionally downregulated in colorectal cancer. We found that CRAD stabilizes the cadherin-catenin-actin complex via capping protein inhibition. The loss of CRAD inhibits F-actin polymerization and subsequently disrupts the cadherin-catenin-actin complex, which leads to ß-catenin release and Wnt signalling hyperactivation. In mice, CRAD knockout induces epithelial cell integrity loss and Wnt signalling activation, resulting in the development of intestinal mucinous adenoma. With APC mutation, CRAD knockout initiates and accelerates mucinous and invasive adenoma development in the colorectum. These results define CRAD as a tumour suppressor, the inactivation of which deregulates the cytoskeleton and hyperactivates Wnt signalling thus initiating mucinous colorectal cancer. Our study reveals the unexpected roles of an actin cytoskeletal regulator in maintaining epithelial cell integrity and suppressing tumorigenesis.


Assuntos
Adenocarcinoma Mucinoso/genética , Oxirredutases do Álcool/genética , Neoplasias Colorretais/genética , Citoesqueleto/metabolismo , Regulação Neoplásica da Expressão Gênica , Proteínas dos Microfilamentos/genética , beta Catenina/metabolismo , Adenocarcinoma Mucinoso/metabolismo , Adenocarcinoma Mucinoso/patologia , Adenoma/genética , Adenoma/metabolismo , Adenoma/patologia , Oxirredutases do Álcool/metabolismo , Animais , Linhagem Celular Tumoral , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Células HCT116 , Células HEK293 , Células HT29 , Humanos , Camundongos Endogâmicos BALB C , Camundongos Knockout , Camundongos Nus , Proteínas dos Microfilamentos/metabolismo , Ligação Proteica
19.
Nat Cell Biol ; 20(12): 1421-1433, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30374053

RESUMO

Vesicular acidification and trafficking are associated with various cellular processes. However, their pathologic relevance to cancer remains elusive. We identified transmembrane protein 9 (TMEM9) as a vesicular acidification regulator. TMEM9 is highly upregulated in colorectal cancer. Proteomic and biochemical analyses show that TMEM9 binds to and facilitates assembly of vacuolar-ATPase (v-ATPase), a vacuolar proton pump, resulting in enhanced vesicular acidification and trafficking. TMEM9-v-ATPase hyperactivates Wnt/ß-catenin signalling via lysosomal degradation of adenomatous polyposis coli (APC). Moreover, TMEM9 transactivated by ß-catenin functions as a positive feedback regulator of Wnt signalling in colorectal cancer. Genetic ablation of TMEM9 inhibits colorectal cancer cell proliferation in vitro, ex vivo and in vivo mouse models. Moreover, administration of v-ATPase inhibitors suppresses intestinal tumorigenesis of APC mouse models and human patient-derived xenografts. Our results reveal the unexpected roles of TMEM9-controlled vesicular acidification in hyperactivating Wnt/ß-catenin signalling through APC degradation, and propose the blockade of TMEM9-v-ATPase as a viable option for colorectal cancer treatment.


Assuntos
Transformação Celular Neoplásica/metabolismo , Proteínas de Membrana/metabolismo , ATPases Vacuolares Próton-Translocadoras/metabolismo , Via de Sinalização Wnt , Proteína da Polipose Adenomatosa do Colo/metabolismo , Animais , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Células HCT116 , Células HT29 , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Intestinos/química , Intestinos/patologia , Proteínas de Membrana/genética , Camundongos Endogâmicos BALB C , Camundongos Knockout , Camundongos Nus , Ligação Proteica , Transplante Heterólogo
20.
ACS Appl Mater Interfaces ; 10(44): 38466-38475, 2018 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-30280569

RESUMO

Owing to their promising applications in flexible electronics, researchers have extensively explored flexible and conductive gels. However, these gels have unsatisfactory strength and flexibility as well as easily dry in air. Herein, a rationally designed robust regenerated silk fibroin (RSF)-based gel with significant flexibility and strength, favorable conductivity, and excellent air stability is fabricated by inducing the conformation transition of RSF from random coil to ß-sheet in ionic liquid (IL)/water mixtures. We found that such RSF-based gels have a unique homogeneous network structure of RSF nanofibers, which is likely formed because of evenly distributed cross-links dominated by small-sized ß-sheet domains created during the conformation transition of RSF. Although the unique homogeneous nanostructure/network contributes toward improving the mechanical properties of these gels, it also provides pathways for ionic transport to help the gels preserve high conductivity of ILs. The prepared RSF-based gels display a remarkable air stability and reversible loss/absorption water capability in a wide humidity range environment primarily because of the distinguished combination of the IL and water. Therefore, the novel RSF-based gels hold a great potential in various applications as multifunctional, flexible, conductive materials, which are dispensed with encapsulation.


Assuntos
Fibroínas/química , Géis/química , Nanoestruturas/química , Água/química , Animais , Bombyx/química , Condutividade Elétrica , Fibroínas/farmacologia , Géis/farmacologia , Líquidos Iônicos/química , Fenômenos Mecânicos , Nanofibras/química , Conformação Proteica em Folha beta
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