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1.
Talanta ; 207: 120293, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594564

RESUMO

Droplet digital PCR (ddPCR) has attracted much attention in the detection of genetic signatures of cancer present at low levels in circulating tumor DNA (ctDNA) in blood. A growing number of laboratory-developed liquid biopsy tests based on such technology have become commercially available for clinical settings. To obtain consistent and comparable results, an international standard is necessary for validation of the analytical performance. In this study, a novel and SI-traceable "ctDNA" reference material (RM) carrying BRAF V600E was prepared by gravimetrically mixing a 152 bp PCR amplicon and sonicated wild-type genomic DNA. The ddPCR performance was evaluated by analyzing serial "ctDNA" dilutions using a competitive MGB assay. The mutant frequency concordance (k) between ddPCR and the gravimetrical value was 1.03 in the range from 53.9% to 0.1%. The limit of blank (LoB), detection (LoD) and quantification (LoQ) of ddPCR assay were determined to be 0.01%, 0.02% and 0.1%, respectively. Results from the interlaboratory study, using challenging low levels of BRAF V600E ctDNA RMs, demonstrated that the participating laboratories had the appropriate technical competency to perform accurate ddPCR-based low level of ratio measurements. However, a systematic error caused by uncorrected droplet volume in Naica Crystal ddPCR platform was found by using the ctDNA RM. Between-laboratory consistency in copy number measurement was greatly improved when a correct droplet volume was applied for the ddPCR measurement by using the ctDNA RM. This confirms that the "ctDNA" RM is fit for the validation of ddPCR systems for ctDNA quantification. This would also support translation of tests for circulating tumor DNA by ddPCR into routine use.

2.
Methods Mol Biol ; 2063: 139-154, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31667768

RESUMO

Eukaryotic cell organelles exert unique functions individually but also interact with each other for essential cellular functions. This physical interface between the organelles serves as an important platform for biomolecule trafficking and signaling. Mitochondria are membrane-bound organelles and form a dynamic contact with other organelles. The interactions and communication between mitochondria and endoplasmic reticulum (ER) are facilitated by an ER specific domain, named mitochondria associated ER membrane (MAM). Due to its unique location, the MAM is a "hotspot" for important cell signaling and biochemical processes including calcium homeostasis, lipid synthesis/exchange, inflammasome and autophagosome formation, and mitochondria fission/fusion. Although techniques are available for isolation of organelle fractions including MAM, most utilize animal tissues and cell lines. Here we describe a protocol that is tailored to the isolation of highly purified MAM, mitochondria, ER, and cytosol from human brain. In addition, we include a protocol for the isolation of total RNA and subsequent analysis of microRNAs from these highly purified organelle fractions. Finally, we include a panel of protein markers that are useful for validating the enrichment and purity of each subcellular fraction.

3.
Viruses ; 11(11)2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31717632

RESUMO

There were five outbreaks of H7N9 influenza virus in humans in China since it emerged in 2013, infecting >1000 people. The H7N9 low pathogenic influenza virus was inserted into four amino acids in the HA protein cleavage site to mutate into the H7N9 highly pathogenic virus. This emerging virus caused 15 outbreaks in chickens from the end of 2016 to date. Two H7N9 avian influenza virus (AIV) strains, A/chicken/Guangdong/A46/2013 (LPAIV) and A/chicken/Guangdong/Q29/2017 (HPAIV), were selected to compare the pathogenicity and transmissibility between H7N9 LPAIVs and HPAIVs in chickens. We inoculated 3- to 4-week-old specific-pathogen-free (SPF) chickens with 6 log10EID50/0.1 mL viruses via the ocular-nasal route and co-housed four chickens in each group. The inoculated chicken mortality rate in the A46 and Q29 groups was 1/5 and 5/5, respectively. Q29 virus replication was more efficient compared to the A46 virus in inoculated chickens. Infected chickens initiated viral shedding to naïve contact chickens through respiratory and digestive routes. Both viruses transmitted between chickens by naïve contact, but the Q29 virus had a higher pathogenicity in contact chickens than the A46 virus. Compared with early H7N9 LPAIVs, the pathogenicity and transmissibility of the emerging H7N9 HPAIV was stronger in chickens, indicating that H7N9 influenza virus may continue to threaten human and poultry health.

4.
Neurology ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31719135

RESUMO

OBJECTIVE: To explore the sex differences in outcomes and management after stroke using a large sample with high-quality international trial data. METHODS: Individual participant data were obtained from 5 acute stroke randomized controlled trials. Data were obtained on demographics, medication use, in-hospital treatment, and functional outcome. Study-specific crude and adjusted models were used to estimate sex differences in outcomes and management, and then pooled using random-effects meta-analysis. RESULTS: There were 19,652 participants, of whom 7,721 (40%) were women. After multivariable adjustments, women with ischemic stroke had higher survival at 3-6 months (odds ratio [OR] 0.82, 95% confidence interval [CI] 0.70-0.97), higher likelihood of disability (OR 1.20, 95% CI 1.06-1.36), and worse quality of life (weighted mean difference -0.07, 95% CI -0.09 to 0.04). For management, women were more likely to be admitted to an acute stroke unit (OR 1.17, 95% CI 1.01-1.34), but less likely to be intubated (OR 0.58, 95% CI 0.36-0.93), treated for fever (OR 0.82, 95% CI 0.70-0.95), or admitted to an intensive care unit (OR 0.83, 95% CI 0.74-0.93). For preadmission medications, women had higher odds of being prescribed antihypertensive agents (OR 1.22, 95% CI 1.13-1.31) and lower odds of being prescribed antiplatelets (OR 0.86, 95% CI 0.79-0.93), glucose-lowering agents (OR 0.86, 95% CI 0.78-0.94), or lipid-lowering agents (OR 0.85, 95% CI 0.77-0.94). CONCLUSIONS: This analysis suggests that women who had ischemic stroke had better survival but were also more disabled and had poorer quality of life. Variations in hospital and out-of-hospital management may partly explain the disparities.

5.
Chemosphere ; 242: 125138, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31670000

RESUMO

Pesticides have become an essential tool for pest kill, weed control and microbiome inhibition for both agricultural and domestic use. However, with the massive use, pesticides can exist in soil, air and water, and sometimes even accumulate in the human or other mammals through food chains. Lots of researches have proven that pesticides possess toxicity to mammals on endocrine, neural and immune systems. Autophagy, as a conservative intracellular process, which is activated by stress-related signals, plays a pivotal role, either "angle" or "demon", in regulation of cell fate and function. Recent evidences in researches elucidated a strong link between the autophagy and the toxicity of pesticides. In this review, we summarized the previous researches which focus on the autophagy regulation in the pesticides-induced toxicity, and hope that this work can help us to discover a potential strategy for the treatment of the disease caused by pesticides.

6.
Environ Toxicol ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31696622

RESUMO

Tetrabromoethylcyclohexane (TBECH) has been linked to endocrine disruption, hepatotoxicity, and reproductive toxicity. However, its immunotoxicity remains largely unknown. In the present study, RAW 264.7 cells, mouse macrophage cell line, were exposed to TBECH. MTT assays showed that TBECH significantly enhanced lactate dehydrogenase (LDH) release in RAW 264.7 cells. The mRNA expression of some proapoptotic genes was upregulated by TBECH. Accordingly, TBECH elevated caspase-3 activity. In addition, TBECH upregualted the mRNA levels of some pro-inflammatory cytokines, whereas it downregulated LPS-stimulated mRNA expression of these cytokines. Moreover, TBECH downregulated the mRNA expression of selected antigen presenting-related genes. Furthermore, TBECH increased reactive oxygen species level, reduced glutathione content and the activities of superoxide dismutase and catalase, and upregulated the mRNA expression of selected oxidative stress-related genes. The obtained data demonstrated that TBECH exhibits immunotoxicity in macrophages, and will help to evaluate its health risks.

7.
Math Biosci Eng ; 16(6): 7327-7361, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31698615

RESUMO

Modelling integrated pest management (IPM) with a threshold control strategy can be achieved with a non-smooth Filippov dynamical system coupled by an untreated subsystem and a treated subsystem which includes chemical and biological tactics. The releasing constant of natural enemies related to biological control generates the complex dynamics. Comprehensive qualitative analyses reveal that the treated subsystem exists with transcritical, saddle-node, Hopf and Bogdanov-Takens bifurcations, for which the threshold conditions and bifurcation curves are provided. Further, by applying techniques of non-smooth dynamical systems including the Filippov convex method and sliding bifurcation techniques, we first obtain the sliding dynamic equation, and then we analyze the existence and stability of regular/virtual equilibria, pseudo-equilibria, boundary equilibria, sliding segments and sliding bifurcations. In particular, if we choose the economic threshold (ET) as the bifurcation parameter, then interesting dynamical behaviors, including boundary equilibrium → pseudo-homoclinic → touching → buckling → crossing bifurcations, occur in succession. It is interesting to note that although the number of pests in the untreated subsystem could increase and exceed the economic injury level (EIL), the final size could be less than ET and stabilizes at a relative low level due to side effects of the pesticide on natural enemies. However, the side effects can be effectively avoided by increasing the releasing constant, which can maintain the number of pests below the EIL always and thus achieve the control purpose.

8.
J Neuroinflammation ; 16(1): 210, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31711546

RESUMO

BACKGROUND: The continuum of pro- and anti-inflammatory response elicited by traumatic brain injury (TBI) is suggested to play a key role in the outcome of TBI; however, the underlying mechanisms remain ill -defined. METHODS: Here, we demonstrate that using bone marrow chimeric mice and systemic inhibition of EphA4 receptor shifts the pro-inflammatory milieu to pro-resolving following acute TBI. RESULTS: EphA4 expression is increased in the injured cortex as early as 2 h post-TBI and on CX3CR1gfp-positive cells in the peri-lesion. Systemic inhibition or genetic deletion of EphA4 significantly reduced cortical lesion volume and shifted the inflammatory profile of peripheral-derived immune cells to pro-resolving in the damaged cortex. These findings were consistent with in vitro studies showing EphA4 inhibition or deletion altered the inflammatory state of LPS-stimulated monocyte/macrophages towards anti-inflammatory. Phosphoarray analysis revealed that EphA4 may regulate pro-inflammatory gene expression by suppressing the mTOR, Akt, and NF-κB pathways. Our human metadata analysis further demonstrates increased EPHA4 and pro-inflammatory gene expression, which correlates with reduced AKT concurrent with increased brain injury severity in patients. CONCLUSIONS: Overall, these findings implicate EphA4 as a novel mediator of cortical tissue damage and neuroinflammation following TBI.

9.
Chem Commun (Camb) ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746852

RESUMO

The In(OTf)3-promoted three-component photoredox alkylarylation of styrenes with alkyl NHP esters and arenes to access alkylated arene derivatives through C-C bond cleavage and C-H functionalization is reported. By utilizing visible-light photoredox catalysis, alkyl N-hydroxyphthalimide esters serving as alkyl carbon-centered radicals and a wide range of arenes (e.g., indoles, pyrrole, and electron-rich arenes) as nucleophiles were used to enable the introduction of various alkyl groups and aryl groups across the C[double bond, length as m-dash]C bonds with excellent selectivity and functional group tolerance.

10.
Nat Commun ; 10(1): 4944, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666520

RESUMO

Urban residential buildings make large contributions to energy consumption. Energy consumption per square meter is most widely used to measure energy efficiency in urban residential buildings. This study aims to explore whether it is an appropriate indicator. An extended STIRPAT model was used based on the survey data from 867 households. Here we present that building area per household has a dilution effect on energy consumption per square meter. Neglecting this dilution effect leads to a significant overestimation of the effectiveness of building energy savings standards. Further analysis suggests that the peak of energy consumption per square meter in China's urban residential buildings occurred in 2012 when accounting for the dilution effect, which is 11 years later than it would have occurred without considering the dilution effect. Overall, overlooking the dilution effect may lead to misleading judgments of crucial energy-saving policy tools, as well as the ongoing trend of residential energy consumption in China.

11.
BMC Musculoskelet Disord ; 20(1): 506, 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31679511

RESUMO

BACKGROUND: To evaluate the effectiveness and safety of technology-assisted rehabilitation following total hip/knee replacement (THR/TKR). METHODS: Six electronic databases were searched without language or time restrictions for relevant studies: MEDLINE, EMBASE, Cochrane Library, CINAHL, SPORTDiscus, Physiotherapy Evidence Database (PEDro); from inception to November 7th, 2018. Two reviewers independently applied inclusion criteria to select eligible randomised controlled trials (RCTs) that investigated the effectiveness of technology-based interventions, compared with usual care or no intervention for people undergoing THR/TKR. Two reviewers independently extracted trial details (e.g. patients' profile, intervention, outcomes, attrition and adverse events). Study methodological quality was assessed using the PEDro scale. Quality of evidence was critically appraised using the Grading of Recommendations, Assessment, Development and Evaluation approach. RESULTS: We identified 21 eligible studies assessing telerehabilitation, game- or web-based therapy. There were 17 studies (N = 2188) in post-TKR rehabilitation and 4 studies (N = 783) in post-THR rehabilitation. Compared to usual care, technology-based intervention was more effective in reducing pain (mean difference (MD): - 0.25; 95% confidence interval (CI): - 0.48, - 0.02; moderate evidence) and improving function measured with the timed up-and-go test (MD: -7.03; 95% CI: - 11.18, - 2.88) in people undergoing TKR. No between-group differences were observed in rates of hospital readmissions or treatment-related adverse events (AEs) in those studies. CONCLUSION: There is moderate-quality of evidence showed technology-assisted rehabilitation, in particular, telerehabilitation, results in a statistically significant improvement in pain; and low-quality of evidence for the improvement in functional mobility in people undergoing TKR. The effects were however too small to be clinically significant. For THR, there is very limited low-quality evidence shows no significant effects.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31748784

RESUMO

We found evidence of late-onset Alzheimer disease (LOAD)-associated genetic polymorphism within an exon of Mucin 6 (MUC6) and immediately downstream from another gene: Adaptor Related Protein Complex 2 Subunit Alpha 2 (AP2A2). PCR analyses on genomic DNA samples confirmed that the size of the MUC6 variable number tandem repeat (VNTR) region was highly polymorphic. In a cohort of autopsied subjects with quantitative digital pathology data (n = 119), the size of the polymorphic region was associated with the severity of pTau pathology in neocortex. In a separate replication cohort of autopsied subjects (n = 173), more pTau pathology was again observed in subjects with longer VNTR regions (p = 0.031). Unlike MUC6, AP2A2 is highly expressed in human brain. AP2A2 expression was lower in a subset analysis of brain samples from persons with longer versus shorter VNTR regions (p = 0.014 normalizing with AP2B1 expression). Double-label immunofluorescence studies showed that AP2A2 protein often colocalized with neurofibrillary tangles in LOAD but was not colocalized with pTau proteinopathy in progressive supranuclear palsy, or with TDP-43 proteinopathy. In summary, polymorphism in a repeat-rich region near AP2A2 was associated with neocortical pTau proteinopathy (because of the unique repeats, prior genome-wide association studies were probably unable to detect this association), and AP2A2 was often colocalized with neurofibrillary tangles in LOAD.

13.
J Clin Invest ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31689239

RESUMO

Leptomeningeal anastomoses or pial collateral vessels play a critical role in cerebral blood flow (CBF) restoration following ischemic stroke. The magnitude of this adaptive response is postulated to be controlled by the endothelium, although the underlying molecular mechanisms remain under investigation. Here we demonstrated that endothelial genetic deletion, using EphA4f/f/Tie2-Cre and EphA4f/f/VeCahderin-CreERT2 mice and vessel painting strategies, implicated EphA4 receptor tyrosine kinase as a major suppressor of pial collateral remodeling, CBF and functional recovery following permanent middle cerebral artery occlusion. Pial collateral remodeling is limited by the cross talk between EphA4-Tie2 signaling in vascular endothelial cells, which is mediated through p-Akt regulation. Furthermore, peptide inhibition of EphA4 resulted in acceleration of the pial arteriogenic response. Our findings demonstrate EphA4 is a negative regulator of Tie2 receptor signaling which limits pial collateral arteriogenesis following cerebrovascular occlusion. Therapeutic targeting of EphA4 and/or Tie2 represents an attractive new strategy for improving collateral function, neural tissue health and functional recovery following ischemic stroke.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31774651

RESUMO

Microwave soft magnetic films (SMFs) are the key materials to effectively miniaturize and multifunctionalize the microwave electromagnetic components and devices. However, currently single-layer SMFs encounter a frequency bottleneck at around 10 GHz. The ferromagnet/non-magnetic spacer/ferromagnet sandwich films with strong interlayer exchange coupling are possible solutions to break through that frequency limitation because they exhibit ultrahigh optical mode (OM) resonance frequency f_r^O up to 50 GHz, while the tiny permeability and the limited thickness are their own obstacles to overcome. In this study, biquadratic coupled FeCoB25nm/Ru0.25nm/FeCoB25nm sandwich films with uniaxial magnetic anisotropy were deposited by a composition gradient sputtering method. A pure OM resonance with self-bias f_r^O up to 18.21 GHz and a relative permeability µ_r^O as high as 169 at the cut-off frequency was achieved. Moreover, both the f_r^O and µ_r^O remain unchanged in the magnetic field range of 0-80 Oe, indicating a strong anti-interference capability to small interference field. These results demonstrate that the biquadratic coupled OM resonance can solve the current frequency bottleneck of microwave SMFs by providing ultrahigh resonance frequency while maintaining considerable permeability, thus lead to potential applications of OM resonance in Ku-band microwave magnetic components.

15.
Dalton Trans ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31777910

RESUMO

Efficient removal of radioactive 90Sr from nuclear waste solutions and natural water systems is of vital importance due to its radioactive nature and high mobility. We present here an anionic layered compound (NC4H12)(NC2H8)2[In3(pydc)6]·13.1H2O (SZ-6; pydc = 2,5-pyridinedicarboxylic acid) with the potential remediation ability towards radioactive Sr2+ from seawater. This material exhibits excellent ß and γ radiation resistance both in air and in aqueous solutions. Besides, this material could maintain its structural integrity in real seawater for 77 days. The adsorption experiment results show that SZ-6 exhibits superior Sr2+ removal capability over a wide pH range from 4 to 12 with fast adsorption kinetics and high selectivity. The effective removal of 90Sr from real seawater was demonstrated as well. Our results strongly suggest the potential application of SZ-6 for selectively capturing radionuclides in natural water systems.

16.
J Am Chem Soc ; 141(45): 18075-18082, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31638806

RESUMO

Defect passivation using oxygen has been identified as an efficient and convenient approach to suppress nonradiative recombination and improve the photovoltaic performance of hybrid organic-inorganic halide perovskites (HHPs). However, oxygen can seriously undermine the chemical stability of HHPs due to the reaction of superoxide with protonated organic cations such as CH3NH3+ and [(NH2)2CH]+, thus hindering the deep understanding of how oxygen affects their defect properties. Here we substitute free-proton inorganic Cs+ for organic moiety to avoid the negative effect of oxygen and then systematically investigate the oxygen passivation mechanism in all-inorganic halide perovskites (IHPs) from theory to experiment. We find that, in contrast to conventional oxygen molecule passivation just through physisorption on the surface of perovskites, the oxygen atom can provide a better passivation effect due to its stronger interaction with perovskites. The key point to achieve O-passivated perovskites rather than O2 is the dry-air processing condition, which can dissociate the O2 into O during the annealing process. O-passivated IHP solar cells exhibit enhanced power conversion efficiency (PCE) and better air stability than O2-passivated cells. These results not only provide deep insights into the passivation effect of oxygen on perovskites but also demonstrate the great potential of IHPs for high photovoltaic performance with simplified ambient processing.

17.
Immunol Lett ; 216: 89-96, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31644891

RESUMO

Administration of inactivated probiotics has been proved to enhance host immunity. Herein, we aim to explore their potential in modulating systemic autoimmune disorders. The bovine type II collagen (CII)-induced arthritis (CIA) and CII antibody-induced arthritis (CAIA) mice models were used in this study. Heat-killed Lactobacillus reuteri (h-L. reuteri) was administered before or after the induction of CIA. The results indicated that the severity of CIA was alleviated and the prevalence of CIA was decreased in the mice pre-treated with h-L. reuteri. Using enzyme-linked immunosorbent assays, we found that decreased serum CII-specific IgG antibody IL-6 and CXCL1 but the increased level of IL-10 was found in h-L. reuteri-treated cohort. Moreover, h-L. reuteri treatment decreased the severity and incidence of arthritis in the CAIA model which was associated with a early decrease of IL-6. Systematic supplement of exogenous IL-6 reversed h-L. reuteri-induced CIA suppression. For regulatory immune responses, the frequency of Tregs and CD4+IL-10+ cells was increased in the draining lymph of joint of h-L. reuteri-treated mice after second immunization. Parallelly, we found that if CIA was induced, CD103+ dendritic cells in mesenteric lymph nodes and α4ß7+ Tregs in the spleen were increased in h-L. reuteri-treated mice, suggesting h-L. reuteri might affect the peripheral migration of Tregs to modulating CIA. Finally, the mice with progressive CIA were treated with h-L. reuteri after the second immunization. No alleviation of CIA severity, as well as an increase of splenic α4ß7+ Tregs, was observed in these mice. This study indicates that pre-administration of h-L. reuteri can alleviate the CIA in mice and may serve as a promising strategy for autoimmune disease prevention.

18.
Rejuvenation Res ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31591931

RESUMO

One of the widely accepted conjectures regarding mechanisms of aging is probably the oxidative stress hypothesis. ß-1,3-Glucans, well-known immunostimulants, have been shown to increase nonspecific immunity and resistance against infections or pathogenic bacteria in several fish species, but its antiaging function remains poorly understood. By feeding of ß-1,3-glucans to the annual fish, Nothobranchius guentheri, we detected the survivorship of the fish and estimated the development of age-related biomarkers at different stages. We first showed that administration of ß-1,3-glucans was able to prolong the lifespan of the fish (p < 0.05). We then showed that ß-1,3-glucans clearly reduced the accumulation of lipofuscin in the gills and the senescence-associated ß-galactosidase in the caudal fins. Moreover, ß-1,3-glucans were able to lower the levels of protein oxidation, lipid peroxidation, and reactive oxygen species (ROS) in the muscles. Finally, ß-1,3-glucans could promote the activities of the antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase in the fish, and slow down the increase of P66shc, a critical factor involved in the regulation of intracellular ROS contents. These data together suggest for the first time that ß-1,3-glucans can extend the lifespan, delay the onset of age-related biomarkers and exert an antioxidant action of the aged fish, N. guentheri. It also implies that ß-1,3-glucans may be potentially useful for health care in the elderly, including extension of the lifespan.

19.
Sci Rep ; 9(1): 14854, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31619714

RESUMO

B7-H4, as a member of the B7 superfamily, was overexpressed in various types of cancers. However, the effects of B7-H4 on the aggressiveness of HCC and the underlying mechanisms have not yet been fully explored. For this purpose, B7-H4 expression was detected by Flow cytometry and Western blotting, it was highly expressed in several HCC cell lines but not in normal LO2 cell line. Knockdown B7-H4 expression induced HCC cells apoptosis by flow cytometry and colony formation assays and increased several apoptosis-related proteins, including survivin, cleaved caspase-3, cleaved caspase-7, and Bax, while the pro-growth protein survivin was reduced. Then the proliferation and cell cycle were suppressed after treated by siB7-H4. Moreover, the level of B7-H4 was significantly correlated with cell migration. In vivo, intra-tumor injection of siRNA targeting B7-H4 can significantly inhibited the growth of HepG2 cells in nude mice. Finally, regions of interest were manually traced on T1WI, T2WI, DWI and ADC of MR images. ADC values were increased in HCC xenografts after B7-H4 siRNA treatment. These data indicated that downregulation of B7-H4 suppressed the proliferation and migration and promoted apoptosis in vitro and in vivo. Blocking the B7-H4 channel might be a potential therapeutic strategy for HCC.

20.
ACS Appl Mater Interfaces ; 11(43): 39961-39969, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31580054

RESUMO

Transition-metal phosphides have a potential application in lithium-ion batteries (LIBs) because of their high theoretical capacities and low cost; nevertheless, they possess dramatic volumetric variation during cycling associated with poor conductivity, limiting their practical applications. Here, a three-dimensional (3D) hierarchical flowerlike FeP coated with nitrogen-doped carbon layer (FeP@N,C hybrid) was constructed through a solvothermal method, followed by a phosphating approach under low temperature. N-doped carbon not only suppresses the volume fluctuation of FeP, but also promotes electron transfer, accompanied by catalyzing the decomposition of Li3P to improve the reversibility of the FeP@N,C hybrid during cycling processes. In addition, a 3D flowerlike architecture assembled from porous nanosheets is also beneficial for shortening the migration path of ions as well as improving the contact area of electrode with electrolyte, which enhances the reaction kinetics and is proved by both experimental measurement of Li+ diffusion coefficient and resistivity, along with the calculation of density functional theory. Consequently, the 3D hierarchical flowerlike FeP@N,C hybrid performs excellent cyclic stability (569 mA h g-1 at a current density of 500 mA g-1 for the 300th cycle) and rate performance (331.94 mA h g-1 at a high current density of 5 A g-1) for LIBs. Based on above results, the fabrication strategy in this work could offer a thought to design other high-performance metal phosphide hybrids.

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