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1.
Talanta ; 207: 120293, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594564

RESUMO

Droplet digital PCR (ddPCR) has attracted much attention in the detection of genetic signatures of cancer present at low levels in circulating tumor DNA (ctDNA) in blood. A growing number of laboratory-developed liquid biopsy tests based on such technology have become commercially available for clinical settings. To obtain consistent and comparable results, an international standard is necessary for validation of the analytical performance. In this study, a novel and SI-traceable "ctDNA" reference material (RM) carrying BRAF V600E was prepared by gravimetrically mixing a 152 bp PCR amplicon and sonicated wild-type genomic DNA. The ddPCR performance was evaluated by analyzing serial "ctDNA" dilutions using a competitive MGB assay. The mutant frequency concordance (k) between ddPCR and the gravimetrical value was 1.03 in the range from 53.9% to 0.1%. The limit of blank (LoB), detection (LoD) and quantification (LoQ) of ddPCR assay were determined to be 0.01%, 0.02% and 0.1%, respectively. Results from the interlaboratory study, using challenging low levels of BRAF V600E ctDNA RMs, demonstrated that the participating laboratories had the appropriate technical competency to perform accurate ddPCR-based low level of ratio measurements. However, a systematic error caused by uncorrected droplet volume in Naica Crystal ddPCR platform was found by using the ctDNA RM. Between-laboratory consistency in copy number measurement was greatly improved when a correct droplet volume was applied for the ddPCR measurement by using the ctDNA RM. This confirms that the "ctDNA" RM is fit for the validation of ddPCR systems for ctDNA quantification. This would also support translation of tests for circulating tumor DNA by ddPCR into routine use.

2.
Opt Lett ; 44(19): 4853-4856, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31568459

RESUMO

Photonic crystals (PCs) are usually fabricated on bulk substrates which break the symmetry of the PC system for incidence from either side of the PCs. Here we report the fabrication of a free-standing 1D layered dielectric PC by using a two-beam holographic interference method. The free-standing PC exhibits distinct photonic bandgaps as well as Fabry-Perot oscillations in the photonic bands. Furthermore, we show that the PC can be modeled by an effective medium approach and obtain the reflection phase for the photonic bands of the PC. We have also performed full-wave simulations for the PC and obtained very good agreement with the experiment. The free-standing PC enables a better comparison between experiment and simulation, and importantly, it is flexible enabling new applications for PCs.

3.
J Clin Lab Anal ; : e22970, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31568612

RESUMO

BACKGROUND: To analyze the therapeutic effect of high-frequency ultrasound (HFU)-assisted dye laser on hemangioma patients and changes in serum hypoxia-inducible factor-1α (HIF-1α). METHODS: A total of 20 patients diagnosed with hemangioma in our hospital from January 2013 to March 2018 were selected, including 12 males and eight females. All patients were treated with HFU-assisted dye laser. The site and type of hemangioma and age distribution of patients were collected, and changes in data and area of hemangioma and serum HIF-1α before and after treatment were analyzed. RESULTS: The vascular condition of hemangioma in all patients was significantly improved at 7, 14, and 30 days after treatment. Gray-scale ultrasound displayed that the tumor area was reduced by more than 50%. After treatment, the serum HIF-1α level declined obviously after treatment compared with that before treatment, showing a statistically significant difference (P < 0.05). CONCLUSION: HFU-assisted dye laser can effectively reduce the tumor area, decrease the serum HIF-1α level, and improve the prognosis in the treatment of hemangioma.

4.
Rejuvenation Res ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31591931

RESUMO

One of the widely accepted conjectures regarding mechanisms of aging is probably the oxidative stress hypothesis. ß-1,3-Glucans, well-known immunostimulants, have been shown to increase non-specific immunity and resistance against infections or pathogenic bacteria in several fish species, but its anti-aging function remains poorly understood. By feeding of ß-1,3-glucans to the annual fish Nothobranchius guentheri, we detected the survivorship of the fish and estimated the development of age-related biomarkers at different stages. We first showed that administration of ß-1,3-glucans was able to prolong the life span of the fish (p<0.05). We then showed that ß-1,3-glucans clearly reduced the accumulation of lipofuscin in the gills and the senescence-associated-ß-galactosidase in the caudal fins. Moreover, ß-1,3-glucans were able to lower the levels of protein oxidation, lipid peroxidation and reactive oxygen species in the muscles. Finally, ß-1,3-glucans could promote the activities of the antioxidant enzymes, including catalase, superoxide dismutase and glutathione peroxidase in the fish, and slow down the increase of P66shc, a critical factor involved in the regulation of intracellular reactive oxygen species contents. These data together suggest for the first time that ß-1,3-glucans can extend the lifespan, delay the onset of age-related biomarkers and exert an antioxidant action of the aged fish N. guentheri. It also implies that ß-1,3-glucans may be potentially useful for health care in the elderly, including extension of the lifespan.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31580054

RESUMO

Transition metal phosphides (TMPs) have potential application in lithium-ion batteries (LIBs) because of high theoretical capacities and low cost, nevertheless, they possess dramatic volumetric variation during cycling associated with poor conductivity, limiting their practical applications. Here, a three-dimensional (3D) hierarchical flower-like FeP coated with nitrogen-doped carbon layer (FeP@N, C hybrid) was constructed through a solvothermal method, then a phosphating approach under low temperature. N-doped carbon not only suppresses the volume fluctuation of FeP, but also promotes electron transfer, accompanied by catalyzing the decomposition of Li3P to improve reversibility of the FeP@N, C hybrid during cycling processes. Additionally, 3D flower-like architecture assembled from porous nanosheets is also beneficial for shortening the migration path of ions as well as improving contact area of electrode with electrolyte, which enhance the reaction kinetics, proved by both experimental measurement of Li+ diffusion coefficient and resistivity, along with calculation of density functional theory (DFT). Consequently, the 3D hierarchical flower-like FeP@N, C hybrid performs excellent cyclic stability (569 mAh g-1 at a current density of 500 mA g-1 for the 300th cycle) and rate performance (331.94 mAh g-1 at a high current density of 5 A g-1) for LIBs. Based on above results, the fabrication strategy in this work could offer a thought to design other high-performance metal phosphides hybrids.

6.
ACS Nano ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31560191

RESUMO

Bioinspired mineralization is a powerful method for designing and preparing nanomaterials. In this work, we developed a bioinspired mineralization approach under freezing conditions and fabricated methyl cellulose (MC)/NaHCO3 flake precursors with a sophisticated hierarchical structure. Based on this, amazing wing-like porous carbon sheets (WPCSs) assembled by numerous interconnected hollow carbon bubbles were obtained after carbonization and removal of inorganic crystals, which are seldom obtained by other artificial methods. Benefiting from their open framework, large surface area, and enlarged interlayer spacing of graphitized nanocrystallites, the obtained WPCSs exhibited an obvious boost in potassium storage performance. As an anode of potassium-ion batteries, they showed high reversible capacities of 347 mAh g-1 at 50 mA g-1 and 122 mAh g-1 at 20 A g-1 and relatively stable cyclability for 3000 cycles. The assembled WPCS//WPCS potassium-ion hybrid supercapacitor delivered a high energy density of 108 Wh kg-1 at a power density of 280 W kg-1. Given the cost effectiveness and green process, the modified bioinspired mineralization under freezing conditions would provide a facile and green way for exploring porous carbons with controlled structures and rich multifunction.

7.
ACS Appl Mater Interfaces ; 11(38): 35105-35114, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31474105

RESUMO

Solar-blind photodetectors have captured intense attention due to their high significance in ultraviolet astronomy and biological detection. However, most of the solar-blind photodetectors have not shown extraordinary advantages in weak light signal detection because the forewarning of low-dose deep-ultraviolet radiation is so important for the human immune system. In this study, a high-performance solar-blind photodetector is constructed based on the n-Ga2O3/p-CuSCN core-shell microwire heterojunction by a simple immersion method. In comparison with the single device of the Ga2O3 and CuSCN, the heterojunction photodetector demonstrates an enhanced photoelectric performance with an ultralow dark current of 1.03 pA, high photo-to-dark current ratio of 4.14 × 104, and high rejection ratio (R254/R365) of 1.15 × 104 under a bias of 5 V. Excitingly, the heterostructure photodetector shows high sensitivity to the weak signal (1.5 µW/cm2) of deep ultraviolet and high-resolution detection to the subtle change of signal intensity (1.0 µW/cm2). Under the illumination with 254 nm light at 5 V, the photodetector shows a large responsivity of 13.3 mA/W, superb detectivity of 9.43 × 1011 Jones, and fast response speed with a rise time of 62 ms and decay time of 35 ms. Additionally, the photodetector can work without an external power supply and has specific solar-blind spectrum selectivity as well as excellent stability even through 1 month of storage. Such prominent photodetection, profited by the novel geometric construction and the built-in electric field originating from the p-n heterojunction, meets greatly well the "5S" requirements of the photodetector for practical application.

8.
Chemosphere ; 236: 124413, 2019 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-31545206

RESUMO

TBBPA is one of the main brominated flame retardants and is ubiquitous in the environment. TBBPA can directly encounter immune cells via the bloodstream, posing potential immunotoxicity. To understand the immunomodulating effect of TBBPA on macrophages, the murine macrophages, RAW 264.7, were exposed to TBBPA at environmentally relevant concentrations (1-100 nM). The results showed that TBBPA at the selected concentrations did not alter cell viability of RAW 264.7 cells with or without LPS stimulation. TBBPA upregulated the expression of pro-inflammatory cytokines, including IL-1ß, IL-6, and TNF-α, whereas it attenuated the LPS-stimulated expression of these pro-inflammatory cytokines, and the expression of anti-inflammatory cytokines, including IL-4, IL-10, and IL-13. In addition, TBBPA reduced the mRNA levels of antigen-presenting-related genes, including H2-K2, H2-Aa, Cd80, and Cd86. Moreover, TBBPA impaired the phagocytic activity of macrophages. Furthermore, exposure to TBBPA significantly elevated the protein levels of phosphorylated NF-κB p65 (p-p65), while it reduced LPS-stimulated p-p65 protein levels. DCFH-DA staining assays showed that TBBPA caused a slight but significant elevation in reactive oxygen species levels. The data obtained in the present study demonstrated that exposure to environmentally relevant concentrations of TBBPA posed immunotoxicity in macrophages and unveiled a potential health risk of TBBPA.

9.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(9): 861-867, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31506143

RESUMO

OBJECTIVE: To examine the levels of airway inflammatory mediators in peripheral blood in infants and young children with wheezing and to study the possible pathogenesis of wheezing from the aspects of T helper cell 1 (Th1)/T helper cell 2 (Th2) imbalance and airway inflammation. METHODS: A total of 50 children aged 1 month to 3 years with an acute wheezing episode were enrolled as the wheezing group, and 25 age-matched healthy infants were enrolled as the healthy control group. According to the number of wheezing episodes, the wheezing group was divided into a first-episode group (n=25) and a recurrent wheezing (number of episodes ≥2) group (n=25). According to the presence or absence of high-risk factors for asthma, the wheezing group was divided into a high-risk factor group (n=22) and a non-high-risk factor group (n=28). According to the results of pathogen detection, the wheezing group was divided into a positive pathogen group (n=23) and a negative pathogen group (n=27). Levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13), transforming growth factor-ß1 (TGF-ß1), and total IgE (TIgE) in peripheral blood were measured for each group. For children with wheezing, eosinophil (EOS) count in peripheral blood was measured, and related samples were collected for respiratory pathogen detection. RESULTS: The wheezing group had significantly higher levels of IL-4, IL-5, IL-13, TGF-ß1, and TIgE in peripheral blood than the healthy control group (P<0.05). There were no significant differences in the levels of IL-2, IL-4, IL-5, IL-13, TGF-ß1, and TIgE in peripheral blood between the first-episode and recurrent wheezing groups, between the high-risk factor and non-high-risk factor groups, and between the positive pathogen and negative pathogen groups (P>0.05). The correlation analysis showed that in children with wheezing, EOS count was positively correlated with IL-4 level (P<0.01), IL-4 level was positively correlated with IL-5 and IL-13 levels (P<0.01), IL-5 level was positively correlated with IL-13 level (P<0.01), and IL-2 level was positively correlated with TGF-ß1 level (P<0.05). CONCLUSIONS: Th1/Th2 imbalance with a predominance of Th2 is observed in infants and young children with wheezing. IL-4, IL-5, IL-13, TGF-ß1, and IgE are involved in the pathogenesis of wheezing in these children. Airway inflammation is also observed in these children with wheezing, but it is not associated with the number of wheezing episodes, presence or absence of high-risk factors for asthma, or results of pathogen detection.


Assuntos
Asma , Sons Respiratórios , Criança , Pré-Escolar , Humanos , Lactente , Mediadores da Inflamação , Interleucina-13 , Células Th1
10.
Carbohydr Polym ; 225: 115252, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31521309

RESUMO

A long-anticipated cancer therapy would deliver the right type of therapeutic agents to the target in control with minimal systemic toxicity. The purpose of this study was to prepare lactosylated curdlan-triornithine nanocarriers (CTOLs), and target deliver gene to hepatoma cells. Structures and biophysical properties had been elucidated with physical and chemical methods. The results revealed that those functionalized polymers can completely condense the gene into spherical nanoparticles. Cytotoxicity assay, GFP-pDNA and siRNA transfection in vitro were implemented successively. Observations showed that CTOL 20% with the highest lactose acid substitution degree targeted delivered gene into HepG2 cells over expressing ASGPR receptors and had pretty gene knockdown efficiency over 70%. Meanwhile, the carriers showed excellent biocompatibility. Our studies demonstrated that CTOLs with lower toxicity and higher gene binding capacity may serve as a potential valuable platform that can be tailored to target the liver cancer cells for therapeutic gene.

11.
Biomed Res Int ; 2019: 6282635, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31531360

RESUMO

Quercetin, a natural flavonol existing in many food resources, has been reported to be an effective antimicrobial and anti-inflammatory agent for restricting the inflammation in periodontitis. In this study, we aimed to investigate the anti-inflammatory effects of quercetin on Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide- (LPS-) stimulated human gingival fibroblasts (HGFs). HGFs were pretreated with quercetin prior to LPS stimulation. Cell viability was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The levels of inflammatory cytokines, including interleukin-1ß (IL-1ß), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), along with chemokine interleukin-8 (IL-8), were determined by enzyme-linked immunosorbent assay (ELISA). The mRNA levels of IL-1ß, IL-6, IL-8, TNF-α, IκBα, p65 subunit of nuclear factor-kappa B (NF-κB), peroxisome proliferator-activated receptor-γ (PPAR-γ), liver X receptor α (LXRα), and Toll-like receptor 4 (TLR4) were measured by real-time quantitative PCR (RT-qPCR). The protein levels of IκBα, p-IκBα, p65, p-p65, PPAR-γ, LXRα, and TLR4 were characterized by Western blotting. Our results demonstrated that quercetin inhibited the LPS-induced production of IL-1ß, IL-6, IL-8, and TNF-α in a dose-dependent manner. It also suppressed LPS-induced NF-κB activation mediated by TLR4. Moreover, the anti-inflammatory effects of quercetin were reversed by the PPAR-γ antagonist of GW9662. In conclusion, these results suggested that quercetin attenuated the production of IL-1ß, IL-6, IL-8, and TNF-α in P. gingivalis LPS-treated HGFs by activating PPAR-γ which subsequently suppressed the activation of NF-κB.

12.
Appl Opt ; 58(18): 4947-4955, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31503816

RESUMO

We introduce and verify a single-channel time-division filtering low-light-level (LLL) color night vision system (3LCNV). The imaging scheme, comprising a tunable liquid crystal filter, three-generation GaAsP image intensifier, and CMOS camera, achieves LLL color imaging and ensures sensitivity. The image enhancement and color reconstruction algorithm flow suitable for LLL night vision combines overexposure-against white balance, color correction matrix (CCM) color correction, and color image denoising to improve color visibility and reduce color difference and image noise. The proposed night vision system extends the minimum working illuminance to 10-4 lx and achieves natural and clear color LLL imaging, improving night-time observations.

13.
Opt Express ; 27(15): 21717-21728, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31510243

RESUMO

Surface plasmon resonance (SPR) has found wide applications in sensing down to molecular level due to its extreme sensitivity to change of dielectric properties. An unavoidable effect in SPR is surface deformation (thermal bump) due to local heating by incident laser light used in SPR. In addition, changes in the reflection phase from the metal film used in SPR could also contribute to the SPR signal, and thus proper handling of the SPR signal is very important in order to broaden the potential applications of SPR. Here we report a simple Fabry Perot (FP) interference technique for measuring, simultaneously, the thermal bump height as well as the reflection phase shift of gold film used in SPR. We find that the shift of the FP signal is dominated by the effect of the thermal bump while it is small for the effect of the reflection phase shift due to change of dielectric property of the metal. To support our experimental results, we have also performed model simulation for the SPR system and obtain good agreement with the experiment. As both amplitude and phase can be measured, our method could lead to better characterization of SPR and can also be applied to the study of active metasurfaces under external excitation.

15.
Nano Lett ; 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31558023

RESUMO

Pyroptosis is a lytic and inflammatory form of programmed cell death and could be induced by chemotherapy drugs via caspase-3 mediation. However, the key protein gasdermin E (GSDME, translated by the DFNA5 gene) during the caspase-3-mediated pyroptosis process is absent in most tumor cells because of the hypermethylation of DFNA5 (deafness autosomal dominant 5) gene. Here, we develop a strategy of combining decitabine (DAC) with chemotherapy nanodrugs to trigger pyroptosis of tumor cells by epigenetics, further enhancing the immunological effect of chemotherapy. DAC is pre-performed with specific tumor-bearing mice for demethylation of the DFNA5 gene in tumor cells. Subsequently, a commonly used tumor-targeting nanoliposome loaded with cisplatin (LipoDDP) is used to administrate drugs for activating the caspase-3 pathway in tumor cells and trigger pyroptosis. Experiments demonstrate that the reversal of GSDME silencing in tumor cells is achieved and facilitates the occurrence of pyroptosis. According to the anti-tumor activities, anti-metastasis results, and inhibition of recurrence, this pyroptosis-based chemotherapy strategy enhances immunological effects of chemotherapy and also provides an important insight into tumor immunotherapy.

16.
Oncogene ; 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31409897

RESUMO

Deregulated expression of circular RNAs (circRNAs) is associated with various human diseases, including many types of cancer. Despite their growing links to cancer, there has been limited characterization of circRNAs in metastatic castration-resistant prostate cancer, the major cause of prostate cancer mortality. Here, through the analysis of an exome-capture RNA-seq dataset from 47 metastatic castration-resistant prostate cancer samples and ribodepletion and RNase R RNA-sequencing of patient-derived xenografts (PDXs) and cell models, we identified 13 circRNAs generated from the key prostate cancer driver gene-androgen receptor (AR). We validated and characterized the top four most abundant, clinically relevant AR circRNAs. Expression of these AR circRNAs was upregulated during castration-resistant progression of PDXs. The upregulation was not due to global increase of circRNA formation in these tumors. Instead, the levels of AR circRNAs correlated strongly with that of the linear AR transcripts (both AR and AR variants) in clinical samples and PDXs, indicating a transcriptional mechanism of regulation. In cultured cells, androgen suppressed the expression of these AR circRNAs and the linear AR transcripts, and the suppression was attenuated by an antiandrogen. Using nuclear/cytoplasmic fractionation and RNA in-situ hybridization assays, we demonstrated predominant cytoplasmic localization of these AR circRNAs, indicating likely cytoplasmic functions. Overall, this is the first comprehensive characterization of circRNAs arising from the AR gene. With greater resistance to exoribonuclease compared to the linear AR transcripts and detectability of AR circRNAs in patient plasma, these AR circRNAs may serve as surrogate circulating markers for AR/AR-variant expression and castration-resistant prostate cancer progression.

17.
ACS Appl Mater Interfaces ; 11(35): 32469-32474, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31409071

RESUMO

The third-order optical nonlinearities and the hot electron relaxation time (τ) of random-distributed gold nanorods arrays on glass (R-GNRA) have been investigated by using Z-scan and optical Kerr effect (OKE) techniques. Large third-order optical susceptibility (χ(3)) with the value of 2.5 × 10-6 esu has been obtained around the plamsonic resonance peak under the excitation power intensity of 0.1 GW/cm2. Further decrease of the excitation power intensity down to 0.3 MW/cm2 will lead to the significant increase of χ(3) up to 6.4 × 10-4 esu. The OKE results show that the relaxation time of R-GNRA around the plasmonic peak is 13.9 ± 0.4 ps, which is more than 4 times longer than those of the individual gold nanostructures distributed in water solutions. The Finite-difference time domain simulations demonstrate that this large enhancement of χ(3) and slow down of τ are caused by the gap-induced large local field enhancement of GNRs dimers in R-GNRA. These significant results offer great opportunities for plasmonic nanostructures in applications of photonic and photocatalytic devices.

18.
Nat Commun ; 10(1): 3892, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31467272

RESUMO

Life experience can leave lasting marks, such as epigenetic changes, in the brain. How life experience is translated into storable epigenetic information remains largely unknown. With unbiased data-driven approaches, we predicted that Egr1, a transcription factor important for memory formation, plays an essential role in brain epigenetic programming. We performed EGR1 ChIP-seq and validated thousands of EGR1 binding sites with methylation patterns established during postnatal brain development. More specifically, these EGR1 binding sites become hypomethylated in mature neurons but remain heavily methylated in glia. We further demonstrated that EGR1 recruits a DNA demethylase TET1 to remove the methylation marks and activate downstream genes. The frontal cortices from the knockout mice lacking Egr1 or Tet1 share strikingly similar profiles in both gene expression and DNA methylation. In summary, our study reveals EGR1 programs the brain methylome together with TET1 providing new insight into how life experience may shape the brain methylome.

19.
Trials ; 20(1): 520, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31429810

RESUMO

BACKGROUND: Coronary artery disease (CAD) is one of the most common types of the cardiovascular disease. Previous pilot trials have suggested that Traditional Chinese Medicine (TCM) has brought clinical benefits for patients with CAD. We will conduct this trial to determine the efficacy and safety of Shenzhu Guanxin Recipe Granules (SGR) for the treatment of patients with CAD. METHODS: This randomized controlled trial recruited 190 patients who were diagnosed with CAD by clinical manifestation and examination and in which coronary computed tomography angiography (CCTA) showed 50-70% stenosis, with soft or mixed plaque types. The included participants were randomly assigned to the case group and control group using a 1:1 allocation ratio; patients in the case group received SGR and usual care, and those in the control group received placebo (6 g/day for 6 months) and usual care. The endpoint of the study included Calcium Coverage Score (CCS), C-reactive protein (CRP) level, and the levels of blood lipids, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6), and ATP-binding membrane cassette transporter A1 (ABCA1) were calculated before recruiting and at the sixth month. The indicators were Seattle Angina Questionnaire (SAQ) and TCM Syndrome Questionnaire scores at 0, 3, and 6 months. DISCUSSION: This clinical trial may provide reliable evidence regarding the clinical effectiveness and safety of SGR therapy for patients with CAD diagnosed by clinical manifestation and examination, in which CCTA showed 50-70% stenosis, with soft or mixed plaque types. TRIAL REGISTRATION: ClinicalTrials.gov, ID: ChiCTR1900020501 . The trial was registered on 25 December 2018.

20.
Artif Cells Nanomed Biotechnol ; 47(1): 3598-3602, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31456424

RESUMO

Objective: To apply biological variation and six Sigma models to evaluate analysis performance of 6 HbA1c analyzers and design the new quality control strategy. Method: We collected data of imprecision and inaccuracy from routine internal quality control (June 2017-December 2017) and proficiency test of NGSP, respectively. The coefficient of variance (CV)% and bias% were plotted in the biological variation and six sigma models. The new quality control strategy was designed by the sigma value and OPSpecs. The quality improvement was guided by the QGI. Results: The analytical performance of 6 HbA1c analyzers in our laboratory were good in the routine model, However, 50% (3/6) and 67% (4/6) of the HbA1c analyzers reached the acceptable level in the biological variation and six Sigma model, respectively. We also design personalized control strategy and promote quality improvement by combining the sigma value, OPSpecs, and QGI. Conclusions: Biological variation model and six sigma model could visually display the performance of 6 HbA1c analyzers and personalized control strategy could be designed based on the sigma value, OPSpecs, and QGI.

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