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1.
ACS Nano ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31990179

RESUMO

Periprosthetic infection is considered the main cause of implant failure, which is expected to be solved by fabricating an antibacterial coating on the surface of the implant. Nevertheless, systemic antibiotic treatment still represents the mainstream method for preventing infection, and few antibacterial coatings are applied clinically. This is because the externally introduced traditional antibacterial coatings suffer from the risk of invalidation and tissue toxicity induced by the consumption of antibacterial agents, degradation, and shedding. In this work, we proposed a rapid photo-sonotherapy by creating an oxygen deficiency on a titanium (Ti) implant through sulfur (S)-doping (Ti-S-TiO2-x), which endowed the implants with great sonodynamic and photothermal ability. Without introducing an external antibacterial coating, it reached a high antibacterial efficiency of 99.995% against Staphylococcus aureus under 15 min near-infrared light and ultrasound treatments. Furthermore, bone infection was successfully treated after combination treatments, and improved osseointegration was observed. Importantly, the S-doped Ti implant immersed in water for 6 months showed an unchanged structure and properties, suggesting that the Ti implant with intrinsic modification showed stable antibacterial performance under exogenous stimuli with a high antibacterial performance in vivo. This photo-sonotherapy based on sulfur doping is also promising for cancer therapy with biosafety.

2.
ACS Appl Mater Interfaces ; 12(4): 4797-4803, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31909585

RESUMO

Cu2+ ions play essential roles in various biological events that occur in the human body. It is important to establish an efficient and reliable detection of Cu2+ ions for people's health. The solution-gated graphene transistors (SGGTs) have been extensively investigated as a promising platform for chemical and biological sensing applications. Herein, highly sensitive and highly selective sensor for Cu2+ ion detection is successfully constructed based on SGGTs with gate electrodes modified by functional carbon quantum dots (CQDs). The sensing mechanism of the sensor is that the coordination of CQDs and Cu2+ ions induces the capacitance change of the electrical double layer (EDL) near the gate electrode and then results in the change of channel current. Compared to other metal ions, Cu2+ ions have an excellent binding nature with CQDs that make it an ultrahigh selective sensor. The CQD-modified sensor achieves excellent Cu2+ ion detection with a minimal level of concentration (1 × 10-14 M), which is several orders of magnitude lower than the values obtained from other conventional detection methods. Interestingly, the device also displays a quick response time on the order of seconds. Due to the functionalized nature of CQDs, SGGTs with CQD-modified gate show good prospects to achieve multifunctional sensing platform in biochemical detections.

3.
Nanoscale ; 12(5): 3435-3442, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31989998

RESUMO

Chinese hydrangea lantern-like Co9S8@MoS2 composites are prepared by a facile solvothermal method. Ultra-thin MoS2 nanosheets as the shells grow tightly and uniformly on the surface of the Co9S8 core. Due to their unique hierarchical core-shell structure and novel morphology, the composites show excellent electrochemical performance as the anode materials of lithium-ion batteries. They can deliver reversible discharge capacities of around 1298, 1150, 1089, 1018 and 941 mA h g-1 at the current densities of 0.1, 0.5, 1, 1.5 and 2.0 A g-1, respectively. Moreover, the Co9S8@MoS2 composites can still maintain a discharge capacity of 1048 mA h g-1 after 300 cycles at a current density of 1.0 A g-1.

4.
Chemosphere ; 240: 124820, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31568942

RESUMO

A novel activated primary tank (APT) with an elutriation unit was developed for recovering carbon by the fermentation and elutriation of primary sludge, and the mechanical elutriation mechanism was analysed by conducting a batch fermentation experiment to improve carbon source recovery. The results indicated that a high stirring velocity gradient could cause sludge disintegration, which could not only shorten the fermentation time, but also increase the production of soluble chemical oxygen demand (SCOD) and volatile fatty acids (VFAs) by 8.3% and 9.5%, respectively. Moreover, mechanical elutriation could also promote the release of SCOD from sludge to water, resulting in an increase in the yield of SCOD by 9.2%, it was observed that elutriation intensity plays a more important role than the elutriation time. The microbial community structure of the fermentation system was influenced by the stirring intensity. The relative abundance of fermentative bacteria in the reactor with a stirring intensity (G) of 160 s-1 was 13.8%, which was significantly higher than that in the reactor with G = 31 s-1 (8.037%), so the accumulation of VFAs and SCOD in the reactor with G = 160 s-1 was improved.


Assuntos
Reatores Biológicos/microbiologia , Carbono/química , Esgotos/química , Veículos Automotores
5.
Analyst ; 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31820746

RESUMO

Owing to its high sensitivity, a solution-gated graphene transistor has rapidly emerged as a cutting edge technology in electrochemical sensing. In this work, composites of gold nanoparticles and reduced graphene oxide were synthesized on a glassy carbon electrode by using the electrodeposition method. A modified glassy carbon electrode was used as the gate electrode and assembled into the solution-gated graphene transistor device along with the graphene channel for a non-invasive glucose detection. The sensing mechanism was based on the change in current in the channel of the device caused by the addition of glucose, of which electro-oxidation on the surface of the gold nanoparticles and reduced graphene oxide led to a change in equivalent gate voltage, and consequently, affected the channel carrier concentration. The self-amplification effect of transistors was utilized in our sensors, which resulted in a detection limit that was 10 times lower than those of conventional electrochemical sensors. Compared to traditional enzymatic transistor sensors, the novel solution-gated graphene transistor nonenzymatic sensors based on gold nanoparticles and reduced graphene oxide demonstrated significant sensing advantages, such as a simple structure, wide linear range from 10 µM to 400 µM and 400 µM to 31 mM, and low detection limit down to 4 µM. The chemicals coexisting in human sweat e.g. sodium chloride, urea, and lactic acid imposed no distinct interference for the glucose detection. Therefore, we achieved a non-invasive detection of glucose in the artificial sweat samples with satisfactory sensing results. This work demonstrates an effective route for non-invasive glucose testing in practical clinical diagnosis by using nonenzymatic, solution-gated graphene transistor devices.

6.
J Physiol Biochem ; 75(4): 607-618, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31786730

RESUMO

Previous studies have shown that stromal interaction molecule1 (STIM1)-mediated store-operated Ca2+ entry (SOCE) contributes to intracellular Ca2+ accumulation in H9C2 cells subjected to hypoxia/reoxygenation(H/R) injury. The aim of the present study was to investigate the effect of resveratrol on STIM1-mediated intracellular Ca2+ accumulation and subsequent cell death in the context of myocardial ischemia/reperfusion (I/R) injury. C57 BL/6 mice were fed with either saline or resveratrol (50 mg/kg daily for 2 weeks) and then subjected to myocardial I/R injury. TTC/Evans Blue staining and TUNEL assay were performed to quantify the infarct size and apoptosis index. The cardiac function was evaluated by echocardiography. Neonatal rat ventricular cardiomyocytes (NRVCs) underwent hypoxia/reoxygenation (H/R) to establish the in vitro model. To achieve over-expression, NRVCs were transfected with STIM1-adenovirus vector. Apoptosis was analyzed by TUNEL assay. Cell viability was measured using MTS assay and cell necrosis was determined by LDH release assay. Intracellular Ca2+ concentration was detected by laser scanning confocal microscopy using a Fluo-3AM probe. Resveratrol significantly reduced apoptosis, decreased infarct size, and improved cardiac function in mice subjected to myocardial I/R injury. In NRVCs, resveratrol also downregulated STIM1 expression accompanied by decreased intracellular Ca2+ accumulation elicited by H/R injury. In addition, resveratrol reduced cell apoptosis, upregulated the Bcl-2, decreased Bax, and cleaved caspase-3 expression. Furthermore, the effects of resveratrol on STIM1-mediated intracellular Ca2+ accumulation, apoptotic proteins, and H/R-induced cell injury were exacerbated by STIM1 over-expression and were partly abolished by SOCE inhibitor SKF96365 in NRVCs in vitro. Our findings demonstrate that resveratrol exerts anti-apoptotic activity and improves cardiac functional recovery following myocardial I/R by inhibiting STIM1-induced intracellular Ca2+ accumulation.

7.
ACS Nano ; 13(11): 13581-13594, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31697055

RESUMO

Using noninvasive stimulation of cells to control cell fate and improve bone regeneration by optical stimulation can achieve the aim of precisely orchestrating biological activities. In this study, we create a fast and repeatable photoelectric-responsive microenvironment around an implant using a bismuth sulfide/hydroxyapatite (BS/HAp) film. The unexpected increase of photocurrent on the BS/HAp film under near-infrared (NIR) light is mainly due to the depletion of holes through PO43- from HAp and interfacial charge transfer by HAp compared with BS. The electrons activate the Na+ channel of mesenchymal stem cells (MSCs) and change the cell adhesion in the intermediate environment. The behavior of MSCs is tuned by changing the photoelectronic microenvironment. RNA sequencing reveals that when photoelectrons transfer to the cell membrane, sodium ions flux and the membrane potential depolarizes to change the cell shape. Meanwhile, calcium ions fluxed and FDE1 was upregulated. Furthermore, the TCF/LEF in the cell nucleus began transcription to regulate the downstream genes involved in osteogenic differentiation, which is performed through the Wnt/Ca2+ signaling pathway. This research has created a biological therapeutic strategy, which can achieve in vitro remotely, precisely, and noninvasively controlling cell differentiation behaviors by tuning the in vivo photoelectric microenvironment using NIR light.

8.
Biomater Sci ; 7(12): 5383-5387, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31626246

RESUMO

Xerogels usually possess a stable structure and have a low swelling rate due to their inferior dynamics. Herein, a xerogel was synthesized by "imitative" click chemistry based on lipoic acid for picking up bacteria from wound sites, and thus accelerating tissue repair. The cross-linking structure of disulfide and thioether inside the xerogel not only exhibited good ductility and intrinsic self-healing performance, but also showed superior biocompatibility. The xerogel captured more than 60% of the bacteria Staphylococcus aureus via strong electrostatic adsorption in the colonies with a bacteria count of 106. In addition, this xerogel can stick to the skin in the form of patches in the wounds during therapy for wound healing and can be easily stripped from the skin after treatment, which makes it appropriate for the portable therapy of bacteria-infected wounds in emergency circumstances.

9.
Nat Commun ; 10(1): 4490, 2019 10 03.
Artigo em Inglês | MEDLINE | ID: mdl-31582736

RESUMO

The application of photothermal therapy to treat bacterial infections remains a challenge, as the high temperatures required for bacterial elimination can damage healthy tissues. Here, we develop an exogenous antibacterial agent consisting of zinc-doped Prussian blue (ZnPB) that kills methicillin-resistant Staphylococcus aureus in vitro and in a rat model of cutaneous wound infection. Local heat triggered by the photothermal effect accelerates the release and penetration of ions into the bacteria, resulting in alteration of intracellular metabolic pathways and bacterial killing without systemic toxicity. ZnPB treatment leads to the upregulation of genes involved in tissue remodeling, promotes collagen deposition and enhances wound repair. The efficient photothermal conversion of ZnPB allows the use of relatively few doses and low laser flux, making the platform a potential alternative to current antibiotic therapies against bacterial wound infections.

10.
Biosens Bioelectron ; 146: 111751, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31605988

RESUMO

Detection of nitrite is important for environmental safety and human health, and the development of high-performance sensors for accurate detection of nitrite is highly desirable. Herein, a highly sensitive graphene electrochemical transistor (GECT) nitrite sensor was designed and fabricated for the first time. A single layer of graphene was placed between the source and drain electrodes by the wetting transfer method to act as channel for the transistor. Au nanoparticles modified reduced graphene oxide nanocomposites (AuNPs/RGO) were electrodeposited at the transistor gate to improve its catalytic oxidation performance of nitrite with optimized electrodeposition conditions. The sensing principle was attributed to changes in effective gate voltage applied to GECT induced by electrooxidation of nitrite at gate electrodes. Due to the high carrier mobility of graphene in the channel and the excellent electrocatalytical activity of AuNPs/RGO on the gate, the obtained sensor device exhibited an exceedingly low detection limit (0.1 nM nitrite) and ultra-wide linear range from 0.1 nM to 7 µM and from 7 to 1000 µM, which are comparable or superior to the performance of large-scale instruments (e.g. chromatography, spectrophotometry, and spectrofluorimetry etc.). The GECT device also showed good anti-interference performance toward common interfering ions and stable performances. Nitrite in natural lake water has been proven to be monitored by our devices. Therefore, the present novel GECT sensor could act as a desirable practical platform for highly sensitive detection of nitrite in the food and environmental fields.

11.
Bioresour Technol ; 294: 122228, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31610496

RESUMO

Two aeration modes, namely point aeration and step aeration, were proposed and implemented in a full scale Orbal oxidation ditch, and nitrogen removal performance was studied. The results showed that nitrogen removal performance under point aeration mode depended on oxygen supply control. Highest total nitrogen (TN) removal efficiency of 73.2% was achieved when oxygen input in the outer, middle and inner channel accounted for 50, 25 and 25% of total oxygen supply, respectively. With the same oxygen supply, both aeration modes demonstrated complete nitrification with over 97% ammonia nitrogen removal efficiencies. However, TN removal efficiency was 78.8% under step aeration mode, which was higher than that under point aeration. The pyrosequencing results indicated that microbial community composition was affected by aeration modes and step aeration mode was beneficial to the enrichment of denitrifiers. The greater diversity and relative abundance of denitrifiers enhanced TN removal under step aeration mode.


Assuntos
Desnitrificação , Nitrogênio , Amônia , Reatores Biológicos , Nitrificação , Oxigênio
12.
ACS Cent Sci ; 5(9): 1591-1601, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31572786

RESUMO

Herein, a core-shell dual metal-organic framework (MOF) heterointerface is synthesized. The Prussian blue (PB) MOF acts as a core for the growth of a porphyrin-doped MOF which is named PB@MOF. Porphyrins can significantly enhance the transfer of photoinspired electrons from PB and suppress the recombination of electrons and holes, thus enhancing the photocatalytic properties and consequently promoting the yields of singlet oxygen rapidly under 660 nm illumination. PB@MOF can exhibit a better photothermal conversion efficiency up to 29.9% under 808 nm near-infrared irradiation (NIR). The PB@MOF heterointerface can possess excellent antibacterial efficacies of 99.31% and 98.68% opposed to Staphylococcus aureus and Escherichia coli, separately, under the dual light illumination of 808 nm NIR and 660 nm red light for 10 min. Furthermore, the trace amount of Fe and Zr ions can trigger the immune system to favor wound healing, promising that PB@MOF achieves the rapid therapy of bacterial infected wounds and environmental disinfection.

13.
Adv Sci (Weinh) ; 6(17): 1900599, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31508278

RESUMO

Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS2, IR780 photosensitizer, and arginine-glycine-aspartic acid-cysteine (RGDC) to safely eradicate biofilms on titanium implants within 20 min. The magnetron-sputtered MoS2 film possesses excellent photothermal properties, and IR780 can produce reactive oxygen species (ROS) with the irradiation of near-infrared (NIR, λ = 700-1100 nm) light. Consequently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT), assisted by glutathione oxidation accelerated by NIR light, can provide synergistic and rapid killing of bacteria, i.e., 98.99 ± 0.42% eradication ratio against a Staphylococcus aureus biofilm in vivo within 20 min, which is much greater than that of PTT or PDT alone. With the assistance of ROS, the permeability of damaged bacterial membranes increases, and the damaged bacterial membranes become more sensitive to heat, thus accelerating the leakage of proteins from the bacteria. In addition, RGDC can provide excellent biosafety and osteoconductivity, which is confirmed by in vivo animal experiments.

14.
ACS Omega ; 4(4): 6452-6458, 2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-31459778

RESUMO

Transition-metal oxides are considered as promising anode materials because of the high theoretical specific capacities. However, the fast capacity fading and unstable cycling performance restricted their electrochemical performance. To achieve fast and stable lithium storage capability, in this work, bricklike Ca9Co12O28 is synthesized via a modified Pechini method with the assistance of the C12H25SO4Na surfactant. The as-obtained Ca9Co12O28 ternary oxides exhibit stable structural stability, which may be attributed to the in situ formed CaO layers during the first discharge process. When tested as an anode material in lithium-ion batteries (LIBs), bricklike Ca9Co12O28 exhibits an excellent reversible capacity of 517 mA h g-1 at 1 C after 200 cycles. Even at the high rate of 3 C, the discharge capacity can still reach 392 mA h g-1 after 200 cycles. It reveals a great application prospect in anode materials of LIBs.

15.
ACS Nano ; 13(10): 11153-11167, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31425647

RESUMO

Patients often face the challenge of antibiotic-resistant bacterial infections and lengthy tissue reconstruction after surgery. Herein, human hair-melanosome derivatives (HHMs), comprising keratins and melanins, are developed using a simple "low-temperature alkali heat" method for potentially personalized therapy. The mulberry-shaped HHMs have an average width of ∼270 nm and an average length of ∼700 nm, and the negatively charged HHMs can absorb positively charged Lysozyme (Lyso) to form the HHMs-Lyso composites through electrostatic interaction. These naturally derived biodegradable nanostructures act as exogenous killers to eliminate methicillin-resistant Staphylococcus aureus (MRSA) infection with a high antibacterial efficacy (97.19 ± 2.39%) by synergistic action of photothermy and "Lyso-assisted anti-infection" in vivo. Additionally, HHMs also serve as endogenous regulators of collagen alpha chain proteins through the "protein digestion and absorption" signaling pathway to promote tissue reconstruction, which was confirmed by quantitative proteomic analysis in vivo. Notably, the 13 upregulated collagen alpha chain proteins in the extracellular matrix (ECM) after HHMs treatment demonstrated that keratin from HHMs in collagen-dependent regulatory processes serves as a notable contributor to augmented wound closure. The current paradigm of natural material-tissue interaction regulates the cell-ECM interaction by targeting cell signaling pathways to accelerate tissue repair. This work may provide insight into the protein-level pathways and the potential mechanisms involved in tissue repair.

16.
Analyst ; 144(19): 5802-5809, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31465037

RESUMO

A dual-mode sensing platform, involving fluorescence and reflectance modes, has been demonstrated for highly sensitive and selective detection of solvents and metal ions based on carbon dot-based inverse opal hydrogels (CD-IOHs). In this work, CD-IOHs have been first synthesized via the typical templating technique. Two kinds of CDs, including solvent and Cu(ii) ion sensitive CDs, have been incorporated into the matrix of IOHs during the co-polymerization of acrylic acid (AA) and 2-hydroxyethyl methacrylate (HEMA). The CD-IOHs not only appear green under daylight but also exhibit stable photoluminescence (PL) under UV light owing to the stop-band effect of photonic crystals and the quantum effect of CDs, respectively. By using these two optical phenomena, for solvent sensing, the CD-IOHs change their colors from green, yellow, and red to a semitransparent state and show good linear sensing with the ethanol content varying from 0 to 45% in reflectance mode, while their PL intensities exhibit a nonlinear detection trend: first an increase and then a decrease with the ethanol content in fluorescence mode. Remarkably, as for metal ion sensing, the CD-IOHs have high selectivity for Cu(ii) ions via the specific PL quenching effect of Cu(ii) ion sensitive CDs. Furthermore, the CD-IOHs show good linear detection in both modes and a wide linear detection range from 0.1 µM to 7 mM. Thus, high selectivity, colorimetric detection, a broad linear detection range, and dual-mode sensing can be realized using the CD-IOHs.

17.
Adv Healthc Mater ; 8(19): e1900835, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31464096

RESUMO

Bacterial infections often cause orthopedic surgery failures. It is hard for the immune system and antibiotics to clear bacteria adhered to implants after they form a mature biofilm, and a secondary surgery is required to remove the infected implants. To avoid this, a hybrid coating of Bi2 S3 @Ag3 PO4 /Ti is prepared to eliminate biofilm using near-infrared (NIR) light. Bi2 S3 nanorod (NR) arrays are prepared on titanium (Ti) implants through hydrothermal methods, and Ag3 PO4 nanoparticles (NPs) are loaded on Bi2 S3 NR arrays using a stepwise electrostatic adsorption strategy. The introduction of Ag3 PO4 NPs enhances the photocatalysis performances of Bi2 S3 , and the hybrid coating also exhibits good photothermal effects. After 808 nm light irradiation for 15 min, it shows superior bactericidal efficiency of 99.45% against Staphylococcus aureus, 99.74% against Escherichia coli in vitro, and 94.54% against S. aureus biofilm in vivo. Bi2 S3 @Ag3 PO4 /Ti also shows good cell viability compared to pure Ti. This NIR-activated-inorganic hybrid semiconductor heterojunction coating is biocompatible and could be employed to eliminate biofilm effectively, which makes it a very promising strategy for the surface modification of bone implant materials.

18.
Anal Chim Acta ; 1072: 25-34, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31146862

RESUMO

For the first time, a simple electrochemical co-deposition was utilized to synthesis the gold and zirconia nanocomposites modified graphene nanosheets on glassy carbon electrode (Au-ZrO2-GNs/GCE) for electrocatalytic analysis of methyl parathion (MP). According to Field-Emission Scanning Electron Microscopy (FE-SEM), Transmission Electronic Microscopy (TEM) and X-Ray Diffraction (XRD), the gold nanoparticles were uniformly distributed on the surface of graphene-based nanocomposite. The Au-ZrO2-GNs/GCE based sensor exhibited superior capacity for MP detection, ascribed to the strong affinity of zirconia towards the phosphoric group, as well as the high catalytic activity and good conductivity of Au-GNs. The best fabrication and work conditions were then obtained by systematically optimization of the electrodeposition process, pH value and enrichment time. Compared to the gold nanoparticles, zirconia or graphene modified electrodes, AuZrO2-GNs/GCE sensor displayed superior electro-catalytic response toward MP oxidation. The sensor response current of square wave voltammetry was highly linearly correlated with the MP concentrations range of 1-100 ng mL-1 and 100-2400 ng mL-1 with the detection limit of 1 ng mL-1. The Au-ZrO2-GNs/GCE nanocomposite sensor showed excellent accuracy and reproducibility for detection of MP in Chinese cabbage samples, providing a new method for efficient pesticide detection in practical applications.


Assuntos
Técnicas Eletroquímicas , Ouro/química , Grafite/química , Metil Paration/análise , Nanocompostos/química , Zircônio/química , Eletrodos , Tamanho da Partícula , Propriedades de Superfície
19.
Chem Biol Interact ; 307: 82-90, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31047918

RESUMO

Honokiol (HKL) is a natural low-molecular-weight biphenolic compound derived from the bark of magnolia trees. Previous studies indicate that HKL exerts potent cardioprotective effects on ischemia/reperfusion (I/R) injury; however, evidence of the further relationship between HKL posttreatment and myocardial I/R injury has not been clearly found. In our study, we explored the protective effect of HKL post treatment on myocardial I/R injury in C57BL/6 mice. We also demonstrated that HKL significantly reduced cellular reactive oxygen species production and attenuated mitochondrial damage in neonatal rat cardiomyocytes exposed to hypoxia/reoxygenation (H/R). In addition, HKL was found to enhance autophagy during I/R or H/R; these effects could be partially blocked by the autophagic flux inhibitor chloroquine. Moreover, our results suggested that enhanced autophagic flux is associated with the Akt signaling pathway. Collectively, our results indicate that HKL posttreatment alleviates myocardial I/R injury and suggest a critical cardioprotective role of HKL in promoting autophagic flux.


Assuntos
Autofagia , Compostos de Bifenilo/uso terapêutico , Lignanas/uso terapêutico , Traumatismo por Reperfusão Miocárdica/tratamento farmacológico , Espécies Reativas de Oxigênio/metabolismo , Animais , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Compostos de Bifenilo/química , Compostos de Bifenilo/farmacologia , Cloroquina/farmacologia , Modelos Animais de Doenças , Lignanas/química , Lignanas/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Traumatismo por Reperfusão Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/patologia , Miócitos Cardíacos/citologia , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Transdução de Sinais/efeitos dos fármacos
20.
Biosens Bioelectron ; 136: 91-96, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31039492

RESUMO

Highly sensitive and low-cost DNA detection are in great desire owing to the significant role of DNA molecules in detecting genetic damage and errors for the diagnosis and treatment of multiple diseases. Traditional detections of DNA mainly rely on large-scale instrument, which requires the complicated detection process and high cost. Solution-gated electrochemical transistors are widely studied due to high sensitivity and low cost. Here, we exploit a graphene electrochemical transistor for the efficient and sensitive DNA detection. The probe DNA is modified on the gate electrode to detect the target DNA molecules. A novel method is introduced to modify the gate electrode with the probe DNA to detect the different concentration of target DNA. Herein, in comparison with previous reported methods, our DNA sensors show a good limit of detection in the range of 1 fM - 5 µM. The highly sensitive and selective validate the developed DNA sensor as a promising tool for routine use.


Assuntos
Técnicas Biossensoriais/métodos , DNA/análise , Transistores Eletrônicos , Eletrodos , Ouro/química , Grafite/química
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