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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120346, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34508928

RESUMO

Hg(II) is one of the most toxic heavy metal ions. The bioconcentration and degradation-resistant of Hg(II) bring about serious harm to the ecosystem and humans. Therefore, the establishment of an accurate and effective method for detecting mercury ions is of great significance to environmental protection, food safety and human health. In this work, a new fluorescent nanoprobe was presented using nitrogen-doped carbon quantum dots (N-CQDs) for Hg(II) sensing with high stability and selectivity. On this basis, a paper-based chip was innovatively developed for visualization detection of Hg(II). The N-CQDs were prepared through a one-step hydrothermal reaction using catechol and ethylenediamine as carbon and nitrogen sources, respectively. As-prepared N-CQDs exhibit the strong green fluorescence at the excitation/emission wavelength of 370/511 nm. In aqueous solution, a rapid and highly sensitive detection method of Hg(II) was established by the joint of dynamic and static quenching effect of Hg(II) on N-CQDs fluorescence. Under the optimized conditions, there was a stable correlation between the fluorescence intensity change of N-CQDs and the concentrations of Hg(II) in the range of 15 âˆ¼ 104 nM, and the detection limit was down to 8 nM (S/N = 3). The recoveries of water, sorghum and rice were 91.60 to 102.46%, which was consistent with ICP-MS. More importantly, the N-CQDs nanoprobe was further integrated in nitrocellulose membrane to develop paper-based chip for Hg(II) visualization detection, and the detection performance was also excellent. This strategy had significant implications for achieving low-cost, on-site real-time monitoring of mercury (II) in the environment and food.


Assuntos
Mercúrio , Pontos Quânticos , Carbono , Ecossistema , Humanos , Nitrogênio
2.
Mikrochim Acta ; 188(11): 383, 2021 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-34661751

RESUMO

ZIF-67-derived Co nanoparticles supported on N-doped carbon skeletons have been prepared from melamine foam (Co-NPs/NCs) for non-enzymatic electrochemical H2O2 detection. The synthesis of Co-NPs/NCs was demonstrated via calcination treatment using melamine foam (MF) and ZIF-67 as precursors. The experimental results show that Co-NPs/NCs composites exhibit eminent catalytic activity toward specific determination of H2O2 with high selectivity and sensitivity (252.43 and 203.88 µA mM-1 cm-2), low LOD (0.12 µM), and wide linear ranges (10-2080 and 2080-11,800 µM). The excellent performance might be ascribed to the synergetic effects of MOF and N-doped carbon skeletons. The carbon skeletons serve as a conductive bridge and provide a large specific surface area, which can facilitate electron transfer and well disperse nanoparticles. This non-enzymatic electrochemical sensor based on Co-NPs/NCs can successfully detect H2O2 secreted by living cells, indicating its great potential in the early diagnosis and pathological exploration of disease.

3.
BMC Infect Dis ; 21(1): 1062, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34645414

RESUMO

BACKGROUND: The incidence of hand foot and mouth disease (HFMD) has increased in recent years, making it a very common childhood illness worldwide. The relationship between different enterovirus genotypes and disease severity is not clearly understood. Given that enteroviruses are transmitted through the gastrointestinal tract, we hypothesized that variation in intestinal microorganisms of the host might play a role in the prognosis of HFMD. METHODS: We carried out a meta-transcriptomic-wide association study of fecal samples obtained from a cohort of children (254 patients, 227 tested positive for enterovirus, including 16 patients co-infectied with 2 kinds of enterovirus) with mild and severe HFMD and healthy controls. RESULTS: We found there was no significant difference in the amount of each virus type between the mild and severe cases. Genes of enterovirus 71 (EV71) and coxsackievirus A (CV-A) from the severe and mild cases did not show significant clustering. Clostridium sp. L2-50 and Bacteroides stercoris ATCC 43183 were enriched in the guts of children with severe HFMD and KEGG enrichment was found between mild and severe cases. CONCLUSIONS: Intestinal microorganisms appear to interact with enterovirus to determine the progression of HFMD. Genes of Bacteroides and Clostridium may be used as predictive markers for a more efficient prognosis and intervention. The enrichment of intestinal bacteria genes with functions may facilitate the development of severe symptoms for HFMD patients.


Assuntos
Enterovirus Humano A , Enterovirus , Microbioma Gastrointestinal , Doença de Mão, Pé e Boca , Bacteroides , Criança , China , Enterovirus/genética , Enterovirus Humano A/genética , Microbioma Gastrointestinal/genética , Doença de Mão, Pé e Boca/epidemiologia , Humanos , Lactente
4.
Biol Pharm Bull ; 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34707025

RESUMO

To investigate the mechanism of the protective effect of tetrahydroxystilbene glucoside (TSG) on nerve cells, an injury model induced by rotenone in PC12 cells was constructed. Cell viability was detected by using CCK8 assay. Apoptosis was detected by using flow cytometry. The mitochondrial membrane potential (MMP) was detected by using the fluorescent probe JC-1. Generation of reactive oxygen species (ROS) in PC12 cells was determined using the CM-H2DCFDA probe. Protein expression in PC12 cells was detected using western blotting. The results showed that TSG (20-100 µM) attenuated the cytotoxic effects of rotenone on PC12 cells. TSG pretreatment attenuated the apoptosis rate, the degradation of PARP and the activation of cleaved caspase 3, which was induced by rotenone. TSG can significantly reduce the effect of rotenone on the reduction of MMP and the expression of cytoC in the cytosolic fraction. TSG attenuated rotenone-induced de-phosphorylation and mitochondrial translocation of cofilin, as well as rotenone-induced accumulation of ROS. The western blot results showed that ROT could decrease the expression level of p-GSK-3ß and p-AKT, and TSG could weaken these effects of rotenone. In addition, TSG increased the expression level of Nrf2 in the nuclear fraction. These results suggest that TSG could protect PC12 cells against rotenone through multiple pathways. Thus, TSG has the potential to become a novel neuroprotective agent.

5.
BMJ Open ; 11(10): e052609, 2021 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-34697118

RESUMO

OBJECTIVE: This study aimed to describe the epidemiological and clinical features and potential factors related to the time to return negative reverse transcriptase (RT)-PCR in discharged paediatric patients with COVID-19. DESIGN: Retrospective cohort study. SETTING: Unscheduled admissions to 12 tertiary hospitals in China. PARTICIPANTS: Two hundred and thirty-three clinical charts of paediatric patients with confirmed diagnosis of COVID-19 admitted from 1 January 2020 to 17 April 2020. PRIMARY AND SECONDARY OUTCOME MEASURES: Primary outcome measures: factors associated with the time to return negative RT-PCR from COVID-19 in paediatric patients. SECONDARY OUTCOME MEASURES: epidemiological and clinical features and laboratory results in paediatric patients. RESULTS: The median age of patients in our cohort was 7.50 (IQR: 2.92-12.17) years, and 133 (57.1%) patients were male. 42 (18.0%) patients were evaluated as asymptomatic, while 162 (69.5%) and 25 (10.7%) patients were classified as mild or moderate, respectively. In Cox regression analysis, longer time to negative RT-PCR was associated with the presence of confirmed infection in family members (HR (95% CI): 0.56 (0.41 to 0.79)). Paediatric patients with emesis symptom had a longer time to return negative (HR (95% CI): 0.33 (0.14 to 0.78)). During hospitalisation, the use of traditional Chinese medicine (TCM) and antiviral drugs at the same time is less conducive to return negative than antiviral drugs alone (HR (95% CI): 0.85 (0.64 to 1.13)). CONCLUSIONS: The mode of transmission might be a critical factor determining the disease severity of COVID-19. Patients with emesis symptom, complications or confirmed infection in family members may have longer healing time than others. However, there were no significant favourable effects from TCM when the patients have received antiviral treatment.


Assuntos
COVID-19 , Criança , Pré-Escolar , Estudos de Coortes , Humanos , Masculino , Estudos Retrospectivos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SARS-CoV-2
6.
Materials (Basel) ; 14(20)2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34683691

RESUMO

The purpose of this paper is to study the effects of different trajectory planning methods on the mechanical properties of components. The scope of the research includes finite element simulation calculation and experimental tests of the actual structure. The test shall be carried out in the whole load range until the failure of the structure occurs. Taking the composite conical shell as an example, a variable angle initial path generation method of the conical shell surface is proposed, and the parallel offset algorithms based on partition and the circumferential averaging are proposed to fill the surface. Then, finite element analysis is carried out for the paths that satisfy the manufacturability requirements, the analysis results show that the maximum deformation and maximum transverse as well as longitudinal stress of fiber of circumferential averaging variable angle path conical shell are reduced by 16.3%, 5.85%, and 19.76%, respectively, of that of the partition variable angle path. Finally, the strength analysis of conical shells manufactured by different trajectory design schemes is carried out through finite element analysis and actual failure tests. The finite element analysis results are in good agreement with the experimental results of the actual structure. The results show that the circumferential uniform variable angle has good quality, and it is proved that the path planning algorithm that coordinates path planning and defect suppression plays an important role in optimizing placement trajectory and improving mechanical properties of parts.

7.
Cells ; 10(9)2021 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-34571871

RESUMO

Notch is a conserved developmental signaling pathway that is dysregulated in many cancer types, most often through constitutive activation. Tumor cells with nuclear accumulation of the active Notch receptor, NICD, generally exhibit enhanced survival while patients experience poorer outcomes. To understand the impact of NICD accumulation during tumorigenesis, we developed a tumor model using the Drosophila ovarian follicular epithelium. Using this system we demonstrated that NICD accumulation contributed to larger tumor growth, reduced apoptosis, increased nuclear size, and fewer incidents of DNA damage without altering ploidy. Using bulk RNA sequencing we identified key genes involved in both a pre- and post- tumor response to NICD accumulation. Among these are genes involved in regulating double-strand break repair, chromosome organization, metabolism, like raptor, which we experimentally validated contributes to early Notch-induced tumor growth. Finally, using single-cell RNA sequencing we identified follicle cell-specific targets in NICD-overexpressing cells which contribute to DNA repair and negative regulation of apoptosis. This valuable tumor model for nuclear NICD accumulation in adult Drosophila follicle cells has allowed us to better understand the specific contribution of nuclear NICD accumulation to cell survival in tumorigenesis and tumor progression.


Assuntos
Núcleo Celular/genética , Sobrevivência Celular/genética , Proteínas de Drosophila/genética , Drosophila/genética , Ovário/patologia , Receptores Notch/genética , Transcrição Genética/genética , Animais , Carcinogênese/genética , Carcinogênese/patologia , Reparo do DNA/genética , Feminino , Receptor Notch1/genética , Transdução de Sinais/genética
8.
Pediatr Investig ; : e12282, 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34540320

RESUMO

Importance: The Coronavirus disease 2019 (COVID-19) global pandemic poses a considerable challenge for pediatricians. Objective: This study aimed to identify the epidemiological characteristics and clinical features of pediatric patients with COVID-19 in China. Methods: This multicenter retrospective study included pediatric patients from 46 hospitals in China, covering 12 provinces and two municipalities. Epidemiological, demographic, clinical, laboratory, treatment, and outcome data were analyzed. Results: In total, 211 pediatric patients with COVID-19 were included in this study. The median age was 7.0 years (range: 22 days to 18 years). Approximately 16.3% of the patients exhibited asymptomatic infections, 23.0% had upper respiratory tract infections, and 60.7% had pneumonia, including two with severe pneumonia and one with critical illness. Approximately 78.7% of the pediatric patients occurred in familial clusters. The most three common symptoms or signs at onset in children with COVID-19 were fever (54.5%), cough (49.3%), and pharyngeal congestion (20.8%). Only 17.6% of the patients presented with decreased lymphocyte count, whereas 13.6% had increased lymphocyte count. Among the patients with pneumonia who exhibited abnormal chest computed tomography findings, 18.2% (23/127) of the patients had no other symptoms. Generally, the chest radiographs showed abnormalities that affected both lungs (49.6%); ground-glass opacity (47.2%) was the most common manifestation. The cure and improvement rates were 86.7% (183/211) and 13.3% (28/211), respectively. Only one patient with an underlying condition received invasive mechanical ventilation; none of the patients died. Interpretation: Similar to adults, children of all age groups are susceptible to COVID-19. Fortunately, most pediatric patients have mild symptoms or remain asymptomatic, despite the high incidence of pneumonia. Decreased proportions of white blood cells and lymphocytes are less frequent in children than in adults.

9.
Semin Cancer Biol ; 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34562587

RESUMO

Polyploidy, a cell status defined as more than two sets of genomic DNA, is a conserved strategy across species that can increase cell size and biosynthetic production, but the functional aspects of polyploidy are nuanced and vary across cell types. Throughout Drosophila developmental stages (embryo, larva, pupa and adult), polyploid cells are present in numerous organs and help orchestrate development while contributing to normal growth, well-being and homeostasis of the organism. Conversely, increasing evidence has shown that polyploid cells are prevalent in Drosophila tumors and play important roles in tumor growth and invasiveness. Here, we summarize the genes and pathways involved in polyploidy during normal and tumorigenic development, the mechanisms underlying polyploidization, and the functional aspects of polyploidy in development, homeostasis and tumorigenesis in the Drosophila model.

10.
Nano Lett ; 21(18): 7806-7814, 2021 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-34463511

RESUMO

Harvesting water from untapped fog is a potential and sustainable solution to freshwater shortages. However, designing high-efficiency fog collectors is still a critical and challenging task. Herein, learning from the unique microstructures and functionalities of the Namib desert beetle, honeycomb, and pitcher plant, we present a multi-bioinspired patterned fog collector with hydrophilic nanofibrous bumps and a hydrophobic slippery substrate for spontaneous and efficient fog collection. Interestingly, hydrophilic nanofibrous bumps display a honeycomb-like cellular grid structure self-assembled from electrospun nanofibers. Notably, the patterned nanofibrous fog collector exhibits superior water-collecting efficiency of 1111 mg cm-2 h-1. The hydrophilic nanofibrous bumps increase the effective fog-collecting area, and the hydrophobic slippery substrate promotes quick transport of collected water in the desired direction reducing the secondary water evaporation, finally achieving rapid directional transport of tiny droplets and high-efficiency water collection. This work opens a new avenue to collect water efficiently and provides clues to research on the multi-bioinspired synergistical optimization strategy.


Assuntos
Besouros , Nanofibras , Animais , Interações Hidrofóbicas e Hidrofílicas , Água
11.
Anim Biotechnol ; : 1-11, 2021 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-34392775

RESUMO

The purpose of this study was to evaluate the neuroprotective effect of leptin on a non-human primate model of cerebral ischemia. A total of 39 Guangxi macaques were used to establish the primate cerebral-ischemia model. HE staining was used to evaluated the pathological changes. Moreover, magnetic resonance imaging was used for the detection of embolic area. The measurements of behavior observation and cerebral infarction area were also performed. They all received autologous thrombus operation. Furthermore, western blot and RT-PCR were also used to detect the protein and mRNA expression levels of apoptosis-related factors. Our results showed that leptin could reduce the volume of cerebral infarction by about 35%. Behavioral defects can be significantly improved. In addition, mid-term and long-term behavioral deficiencies had been significantly improved by leptin. Moreover, leptin significantly decreased the expression levels of caspase-3 and Bax, and increased the expression levels of Bcl-2. In conclusion, leptin has neuroprotective effects on cerebral ischemia by effectively reducing the volume of cerebral infarction.

12.
Front Pediatr ; 9: 564991, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34386463

RESUMO

Background: Nosocomial meningitis with multidrug-resistant (MDR) or extensively drug-resistant (XDR) Acinetobacter baumannii is a life-threatening complication in neurosurgery. Treatment of these infections is challenging because of poor penetration of the available antibiotics into the cerebrospinal fluid (CSF). Intrathecal (ITH) or intraventricular (IVT) administration of antibiotics is increasingly used as the last treatment option against MDR/XDR Gram-negative bacteria meningitis not responding to intravenous (IV) regimens. However, pertinent data in pediatric patients is scarce. Case Presentation: A 14-year-old male patient developed meningitis from an MDR strain of A. baumannii following endoscopic endonasal resection of craniopharyngioma. Despite a combination therapy involving IV tigecycline, we observed clinical and bacteriologic failure. The patient was then successfully treated with an ITH and IV polymyxin B-based combination. Quantification of tigecycline and polymyxin B in CSF was performed with two-dimensional high-performance liquid chromatography (2D-HPLC) and HDLC coupled with tandem mass spectrometry (HPLC-MS/MS), respectively. Adverse drug reactions (neurotoxicity and skin hyperpigmentation), probably induced by polymyxin B, were acceptable and reversible. Conclusions: The case illustrates ITH and IV Polymyxin B-based combination is an optimal therapeutic option against MDR A. baumannii meningitis in this pediatric patient. In the future, real-time PK/PD data obtained from patients during ITH/IVT polymyxin B therapy should be required to optimize polymyxin use with maximal efficacy and minimal adverse effects.

13.
Analyst ; 146(17): 5403-5412, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34346414

RESUMO

Two different electrochemical sensors (Hemin-G4/Au/GCE and Hemin-G4/Au/ITO) were developed and applied to explore the electrocatalytic capacity of H2O2 reduction. Due to the excellent catalytic activity of Hemin-G4 and high conductivity of gold nanoparticles, both electrodes show excellent electrochemical performances towards H2O2 with a low LOD (0.67 µM for Hemin-G4/Au/GCE and 0.65 µM for Hemin-G4/Au/ITO), rapid response (<4 s), and high selectivity and sensitivity (314.33 µA mM-1 cm-2 for Hemin-G4/Au/GCE and 322.22 µA mM-1 cm-2 for Hemin-G4/Au/ITO). The two electrodes allow sensitive capture of H2O2 produced by A549 cells. Compared with the conventional method of detection in cell suspensions, an ITO electrode with a large specific surface area and good biocompatibility can provide a promising platform for cell adhesion, so as to realize real-time and in situ detection of extracellular H2O2. The experimental results show that A549 cells can adhere to the surface of the Hemin-G4/Au/ITO electrode and grow well. This is benefitted from the three-dimensional structure of the Hemin-G4/Au hydrogel, which provides a suitable microenvironment for cell adhesion and growth. Furthermore, the in situ detection shows a faster response time than that of in-solution detection. This is because the H2O2 generated by the cells can be directly captured by the ITO electrode, which avoids diffusion from the solution to the electrode. These results indicate that the self-supporting hydrogel modified ITO electrode has great application prospects in basic biomedical research and continuous dynamic surveillance of diseases.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Técnicas Eletroquímicas , Eletrodos , Ouro , Hidrogéis , Peróxido de Hidrogênio , Compostos de Estanho
14.
Food Addit Contam Part B Surveill ; 14(3): 177-183, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34362289

RESUMO

Although the Chinese diet has become very abundant in the past 30 years, few people know that traditional Chinese diet is exposed to aluminium (Al). A total of 1232 samples were purchased during 2017-2019 and analysed for Al content with an inductively coupled plasma-mass spectrometry (ICP-MS) method. High Al levels were found in deep-fried dough sticks (mean 219 mg/kg), starch products (mean 84.5 mg/kg), and steam bread (mean 28.6 mg/kg). The average dietary Al exposure of residents in North China was 1.82 mg/kg bw/week, lower than the PTWI (provisional tolerable weekly intake). Deep-fried dough sticks (DFDS) are the main Al contributor in North China, providing 28.2% of the daily intake. The P95 dietary exposure to Al from DFDS was 2.3 mg/kg bw/week, exceeding the PTWI. Therefore, more attention should be paid to the health risk of exposure to Al from DFDS and starch products. Over-use of Al associated with food additives should be effectively controlled.


Assuntos
Alumínio , Exposição Dietética , Alumínio/análise , Pão/análise , China , Dieta/efeitos adversos , Contaminação de Alimentos/análise , Humanos
15.
Pharm Dev Technol ; 26(8): 910-921, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34280065

RESUMO

Polycaprolactone-poly (ethylene glycol) block copolymer (PCL-PEG) based nanoparticles were prepared for the intravenous administration of docetaxel (DTX). PCL-PEG-Tyr and PCL-PEG-Ang were synthesized by using tyrosine (Tyr) and angiopep-2 (Ang) as coupling ligands, and dual-modified PCL-PEG-based nanoparticles (PCL-PEG-Tyr/Ang) were prepared. The physicochemical properties, in vitro drug release, in vitro cytotoxicity, in vitro cellular uptake efficiency, in vivo biodistribution and in vivo antitumor efficacy of PCL-PEG-based nanoparticles were investigated. The PCL-PEG-based nanoparticles were spherical with a mean diameter of 100 nm and high encapsulation efficiencies (> 85%). The results of in vitro drug release showed that the PCL-PEG-based nanoparticles loaded with DTX had sustained-release characteristics. For in vitro cytotoxicity tests, the dual-modified PCL-PEG-based nanoparticles (PCL-PEG-Tyr/Ang) demonstrated the minimum IC50 value (2.94 µg/mL) compared with other PCL-PEG-based nanoparticles. In addition, the cellular uptake of coumarin-6 (C6) in HT29 cells was observed and determined in the PCL-PEG-Tyr/Ang nanoparticles group, which was significantly higher than that in the other PCL-PEG-based groups and C6 solution group. The results of in vivo imaging showed that dual-modified PCL-PEG nanoparticles had better tumor targeting than the other PCL-PEG-based nanoparticles. In the HT29 tumor-xenografted nude mice model, DTX-loaded PCL-PEG-Tyr/Ang nanoparticles also had a significantly higher inhibitory efficacy on tumor growth than Taxotere®-treated group. These results indicated that the dual-modified PCL-PEG-based nanoparticles (PCL-PEG-Tyr/Ang) could be a promising anticancer drug delivery system.

16.
Comput Intell Neurosci ; 2021: 5529905, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34285692

RESUMO

Plant species recognition is a critical step in protecting plant diversity. Leaf-based plant species recognition research is important and challenging due to the large within-class difference and between-class similarity of leaves and the rich inconsistent leaves with different sizes, colors, shapes, textures, and venations. Most existing plant leaf recognition methods typically normalize all leaf images to the same size and then recognize them at one scale, which results in unsatisfactory performances. A novel multiscale convolutional neural network with attention (AMSCNN) model is constructed for plant species recognition. In AMSCNN, multiscale convolution is used to learn the low-frequency and high-frequency features of the input images, and an attention mechanism is utilized to capture rich contextual relationships for better feature extraction and improving network training. Extensive experiments on the plant leaf dataset demonstrate the remarkable performance of AMSCNN compared with the hand-crafted feature-based methods and deep-neural network-based methods. The maximum accuracy attained along with AMSCNN is 95.28%.


Assuntos
Redes Neurais de Computação , Folhas de Planta
17.
Front Cell Infect Microbiol ; 11: 663884, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34277466

RESUMO

Background: The pandemic of Coronavirus Disease 2019 (COVID-19) brings new challenges for pediatricians, especially in the differentiation with non-COVID-19 pneumonia in the peak season of pneumonia. We aimed to compare the clinical characteristics of pediatric patients with COVID-19 and other respiratory pathogens infected pneumonias. Methods: We conducted a multi-center, cross-sectional study of pediatric inpatients in China. Based on pathogenic test results, pediatric patients were divided into three groups, including COVID-19 pneumonia group, Non-COVID-19 viral (NCV) pneumonia group and Non-viral (NV) pneumonia group. Their clinical characteristics were compared by Kruskal-Wallis H test or chi-square test. Results: A total of 636 pediatric pneumonia inpatients, among which 87 in COVID-19 group, 194 in NCV group, and 355 in NV group, were included in analysis. Compared with NCV and NV patients, COVID-19 patients were older (median age 6.33, IQR 2.00-12.00 years), and relatively fewer COVID-19 patients presented fever (63.2%), cough (60.9%), shortness of breath (1.1%), and abnormal pulmonary auscultation (18.4%). The results were verified by the comparison of COVID-19, respiratory syncytial virus (RSV) and influenza A (IFA) pneumonia patients. Approximately 42.5%, 44.8%, and 12.6% of the COVID-19 patients presented simply ground-glass opacity (GGO), simply consolidation, and the both changes on computed tomography (CT) scans, respectively; the proportions were similar as those in NCV and NV group (p>0.05). Only 47.1% of COVID-19 patients had both lungs pneumonia, which was significantly lower than that proportion of nearly 80% in the other two groups. COVID-19 patients presented lower proportions of increased white blood cell count (16.5%) and abnormal procalcitonin (PCT) (10.7%), and a higher proportion of decreased lymphocyte count (44.0%) compared with the other two groups. Conclusion: Majority clinical characteristics of pediatric COVID-19 pneumonia patients were milder than non-COVID-19 patients. However, lymphocytopenia remained a prominent feature of COVID-19 pediatric pneumonia.


Assuntos
COVID-19 , Pneumonia , Criança , China/epidemiologia , Estudos Transversais , Humanos , Pulmão/diagnóstico por imagem , Pneumonia/epidemiologia , Estudos Retrospectivos , SARS-CoV-2
18.
ACS Appl Mater Interfaces ; 13(30): 36587-36594, 2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34311547

RESUMO

Freshwater shortage is a critical global issue that needs to be resolved urgently. Efficient water collection from fog provides a promising and sustainable solution to produce clean drinking water, especially in the desert and arid regions. Nature has long served as our best source of inspiration for designing new structures and developing new materials. Herein, we report a strategy to design a novel Janus fog collector with a hydrophilic lizard-skin-like nanofibrous network upper surface and hydrophobic slippery lower surface using a simple and feasible method of coating and electrospinning. We analyze the forming law of the lizard-skin-like nanofibrous network structure on different substrates using electric field simulation. The resulting copper mesh-based Janus fog collector exhibits superior water-collecting efficiency (907 mg cm-2 h-1) and long-term durability, achieving directional transport of tiny droplets and high-efficiency water collection. However, there are few reports on the combination of the lizard-skin-like nanofibrous capillary network and slippery surface for efficient fog collection. Therefore, we believe that this work will open a new avenue to collect water efficiently and also provide clues to research on the lizard-skin-like nanofibrous network structure.

19.
Talanta ; 233: 122554, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34215057

RESUMO

Accurate and effective detection of single-stranded nucleic acids is vital in both disease diagnosis and pathological studies. Hence, we develop a PAMmer-assisted CRISPR/Cas9 system mediated G4-EXPAR (Cas-G4EX) strategy for site-specific detection of ssRNA and ssDNA. PAMmer-assisted CRISPR/Cas9 executes the site-specific cleavage of target ssRNA or ssDNA and released product fragment with the desired sequence at the 3'-terminal. This fragment serves as a primer to activate subsequent sequence-dependent exponential amplification reaction (EXPAR). The G-rich EXPAR products assembles with hemin to form a G-Quadruplex (G4/hemin). G4/hemin catalyzes ABTS-H2O2 system with the appearance of vivid green color, realizing naked-eye analysis. Cas-G4EX integrates the superiority of CRISPR/Cas9 and EXPAR, presenting outstanding site-specific recognition and high-performance amplification efficiency. Meanwhile, the programmability of CRISPR/Cas9 system makes the proposed method become a universal detection paradigm for any ssRNA or ssDNA. Cas-G4EX assay shows the linear relationship from 250 aM to 2.5 nM for ssRNA detection with the actual LOD of 250 aM, and that ranges from 100 aM to 1 nM for ssDNA detection with the actual LOD of 100 aM. Additionally, the acceptable recoveries of 101.48%-109.61% for ssRNA and 93.25%-111.98% for ssDNA in real detection of human serum are obtained for detection of single-strand nucleic acid in real samples. Cas-G4EX also exhibits the excellent discrimination for single-base mutation of single-stranded nucleic acids. Therefore, Cas-G4EX assay provides a promising platform in the applications of molecular diagnosis and pathological analysis.


Assuntos
Sistemas CRISPR-Cas , Peróxido de Hidrogênio , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , DNA de Cadeia Simples/genética , Humanos , RNA
20.
Polymers (Basel) ; 13(11)2021 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-34064107

RESUMO

Microcapsules encapsulated within epoxy as a curing agent have been successfully applied in self-healing materials, in which the healing performance significantly depends on the binding behaviour of the epoxy curing agent with the cement matrix. In this paper, the binding energy was investigated by molecular dynamics simulation, which could overcome the shortcomings of traditional microscopic experimental methods. In addition to the construction of different molecular models of epoxy, curing agents, and dilutants, seven models were established to investigate the effects of chain length, curing agent, and epoxy resin chain direction on the interfacial binding energy. The results showed that an increase of chain length exhibited had limited effect on the binding energy, while the curing agent and the direction of the epoxy significantly affected the interfacial binding energy. Among different factors, the curing agent tetrethylenepentamine exhibited the highest value of interfacial binding energy by an increment of 31.03 kcal/mol, indicating a better binding ability of the microcapsule core and the cement matrix. This study provides a microscopic insight into the interface behaviour between the microcapsule core and the cement matrix.

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