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2.
Medicine (Baltimore) ; 98(32): e16592, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31393358

RESUMO

RATIONALE: Refractory nasopharyngeal carcinoma is challenging to treat and at present there is no standard treatment or any good choice. PATIENT CONCERNS: Although the three patients in our case reports had already underwent multiple treatments before, they still suffered from disease recurrence of nasopharyngeal carcinoma. DIAGNOSIS: They were diagnosed as refractory nasopharyngeal carcinoma. INTERVENTIONS: A continuous infusion of Endostar, an antiangiogenic agent, combined with chemotherapy and radiation therapy was given to treat the patients. OUTCOMES: Patients showed complete or partial response to the combined therapy as evidenced by regression of tumors and decrease in plasma Epstein-Barr virus (EBV) DNA load. LESSONS: Continuous infusions of Endostar in combination with chemotherapy and/or radiation therapy showed promising efficacy and safety. The combination therapy indicates a new approach to treat refractory nasopharyngeal carcinoma.


Assuntos
Inibidores da Angiogênese/uso terapêutico , Endostatinas/uso terapêutico , Carcinoma Nasofaríngeo/tratamento farmacológico , Neoplasias Nasofaríngeas/tratamento farmacológico , Proteínas Recombinantes/uso terapêutico , Adulto , Quimiorradioterapia/métodos , Feminino , Humanos , Infusões Intravenosas , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo/terapia , Neoplasias Nasofaríngeas/patologia , Neoplasias Nasofaríngeas/terapia
3.
CNS Neurosci Ther ; 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31411808

RESUMO

INTRODUCTION: The occurrence of circadian rhythm disorder in patients with Alzheimer's disease (AD) is closely related to the abnormal deposition of amyloid-ß (Aß), and d-Ser2-oxyntomodulin (Oxy) is a protease-resistant oxyntomodulin analogue that has been shown to exert neuroprotective effects. AIMS: This study aimed to explore whether Oxy, a new GLP-1R/GCGR dual receptor agonist, can improve the Aß-induced disrupted circadian rhythm and the role of GLP-1R. METHODS: A mouse wheel-running experiment was performed to explore the circadian rhythm, and western blotting and real-time PCR were performed to assess the expression of the circadian clock genes Bmal1 and Per2. Furthermore, a lentivirus encoding an shGLP-1R-GFP-PURO was used to interfere with GLP-1R gene expression and so explore the role of GLP-1R. RESULTS: The present study has confirmed that Oxy could restore Aß31-35-induced circadian rhythm disorders and improve the abnormal expression of Bmal1 and Per2. After interfering the GLP-1R gene, we found that Oxy could not improve the Aß31-35-induced circadian rhythm disorder and abnormal expression of clock genes. CONCLUSION: This study demonstrated that Oxy could improve Aß31-35-induced circadian rhythm disorders, and GLP-1R plays a critical role. This study thus describes a novel target that may be potentially used in the treatment of AD.

4.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 35(3): 199-203, 2019 May 28.
Artigo em Chinês | MEDLINE | ID: mdl-31257798

RESUMO

OBJECTIVE: To analyze the expression and relationship of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR) in local skin tissues of pressure injury and investigate the possible mechanism of stage 3 pressure injury refractory wound. METHODS: Forty male SD rats were randomly divided into normal control group, compressed 3 d, 5 d, 7 d, and 9 d groups. Stage 3 pressure injury animal model were established by magnet compression. The morphology of skin was observed by HE staining. The expression of VEGF was detected by immunohistochemistry. The expression levels of HIF-1α, VEGF and KDR protein in skin tissue were detected by Western blot. One-way analysis of variance and LSD test were performed on the data. RESULTS: ①The HE results showed that compared with the normal control group, the epidermis of the compressed group was gradually thickened, the number of blood vessels was decreased, the collagen arrangement disordered and inflammatory cells infiltration were increased. ②Immunohistochemical results showed that the expression of VEGF protein in the 3 d group was significantly higher than that in the normal control group (P<0.01). The expression of VEGF protein in the skin tissue of 5 d, 7 d and 9 d groups was lower than that in normal control group (P<0.05). WB results were consistent with immunohistochemistry results. ③WB results showed that the expression of HIF-1α in the skin tissues of the rats in 3 d, 5 d and 7 d groups was higher than that in the normal control group (P<0.01 or P<0.05). The expression of KDR protein was lower than that of the normal control group (P<0.05 or P<0.01). CONCLUSION: HIF-1α mediated reduction of VEGF and KDR protein expression and decreased tissue angiogenesis may be one of the important causes of chronic dysfunction of stage 3 pressure injury.


Assuntos
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Pressão/efeitos adversos , Pele/lesões , Fator A de Crescimento do Endotélio Vascular/metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Animais , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Masculino , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Fator A de Crescimento do Endotélio Vascular/genética , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/genética
5.
J Org Chem ; 84(16): 10349-10361, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31347360

RESUMO

A collection of chiral spirocyclic tetrahydronaphthalene (THN)-oxindole hybrids bearing a quaternary carbon center at the ß-position of THN has been developed. The diastereodivergent direct catalytic Michael-aldol reaction between 3-ylideneoxindole and 2-methylbenzaldehyde was accomplished by using bifunctional tertiary amine. Simply changing the protecting group on the substrate in the organocatalytic cascade reaction led to inverted diastereoselectivity in good yields with a high ee value. To explain the diastereodivergence of the organocatalytic Michael-aldol cascade, we also proposed plausible transition-state models for [4 + 2] annulation based on the observed stereochemistry of the products.

7.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(1): 123-127, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31037915

RESUMO

OBJECTIVE: To summarize the drug sensitivity and its trends of Clostridium difficile diarrhea pathogenic strains in a large tertiary hospital, so as to provide basic reference data for the treatment and control of Clostridium difficile infection. METHODS: There were 73 toxigenic isolates collected from fecal sample of diarrheal patients in West China Hospital of Sichuan University during two periods. One was from August to December in 2015 (44 strains) , and another was from July 2016 to July 2017 (29 strains) . Enhanced nosocomial infection control measures were implemented during the second sample collection period. The toxin gene was amplified by PCR and sequenced for identification. Minimum inhibitory concentration (MIC) of metronidazole, vancomycin, clindamycin, moxifloxacin, rifaximin, fidaxomicin and linezolid were determined using agar double dilution method. We analyzed the drug resistance characteristics of Clostridium difficile and compared the changes of antimicrobial resistance before and after the enhanced control measures implementation. RESULTS: All 73 strains tested were sensitive to metronidazole and vancomycin. Resistance rate to clindamycin, moxifloxacin, tetracycline and rifaximin were 79.5%, 26.0%, 27.4%, and 9.5%, respectively. Fidaxomicin and nitazoxanide were highly susceptible in vitro against these strains with MIC ranges<0.008-0.5 mg/L ( P<0.05). Resistance to clindamycin and moxifloxacin were significantly decreased after enhanced control measures implementation (resistance rates were 99.5% vs. 44.8%, 36.4% vs. 10.3%, P<0.05). Additionally, isolate with decreased susceptibility to tinezolid as MIC 16 mg/L was found. CONCLUSION: Clostridium difficileis highly resistant to clindamycin and quinolones. Since strains remain highly sensitive to metronidazole and vancomycin in our hospital, empirical application is reasonable without routine antimicrobial susceptibility testing.


Assuntos
Infecções por Clostridium , Clostridium difficile , Antibacterianos , China , Humanos , Testes de Sensibilidade Microbiana , Universidades
8.
J Biol Chem ; 294(25): 10006-10017, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31101655

RESUMO

Nucleus accumbens-associated protein-1 (NAC1) is a transcriptional repressor encoded by the NACC1 gene, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance. NAC1 has therefore been explored as a potential therapeutic target for managing malignant tumors. However, effective approaches for effective targeting of this nuclear protein remain elusive. In this study, we identified a core unit consisting of Met7 and Leu90 in NAC1's N-terminal domain (amino acids 1-130), which is critical for its homodimerization and stability. Furthermore, using a combination of computational analysis of the NAC1 dimerization interface and high-throughput screening (HTS) for small molecules that inhibit NAC1 homodimerization, we identified a compound (NIC3) that selectively binds to the conserved Leu-90 of NAC1 and prevents its homodimerization, leading to proteasomal NAC1 degradation. Moreover, we demonstrate that NIC3-mediated down-regulation of NAC1 protein sensitizes drug-resistant tumor cells to conventional chemotherapy and enhances the antimetastatic effect of the antiangiogenic agent bevacizumab both in vitro and in vivo These results suggest that small-molecule inhibitors of NAC1 homodimerization may effectively sensitize cancer cells to some anticancer agents and that NAC1 homodimerization could be further explored as a potential therapeutic target in the development of antineoplastic agents.

9.
Sci Rep ; 9(1): 1083, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30705349

RESUMO

The differences between the XXZ model with topological and periodical boundary conditions were compared by studying their entanglement, quantum discord, and critical temperature above which the entanglement vanishes. It shows that the different boundary conditions mainly affect bipartite quantum correlations of the boundary spins rather than that of other spin pairs. The topological boundary spins can protect entanglement and discord against strong magnetic fields while the periodical boundary spins can protect them against nonuniform magnetic fields. Compared with the periodical XXZ model, the critical temperature is significantly improved for the topological XXZ model. The topological XXZ model also allows us to improve significantly its critical temperature by increasing the strength of magnetic field, which is not feasible for the periodical XXZ model. It is therefore more promising for preparing entangled states at high temperature in the topological XXZ model.

10.
Artigo em Inglês | MEDLINE | ID: mdl-30770441

RESUMO

Trimeric intracellular cation (TRIC) channels are thought to provide counter-ion currents that facilitate the active release of Ca2+ from intracellular stores. TRIC activity is controlled by voltage and Ca2+ modulation, but underlying mechanisms have remained unknown. Here we describe high-resolution crystal structures of vertebrate TRIC-A and TRIC-B channels, both in Ca2+-bound and Ca2+-free states, and we analyze conductance properties in structure-inspired mutagenesis experiments. The TRIC channels are symmetric trimers, wherein we find a pore in each protomer that is gated by a highly conserved lysine residue. In the resting state, Ca2+ binding at the luminal surface of TRIC-A, on its threefold axis, stabilizes lysine blockage of the pores. During active Ca2+ release, luminal Ca2+ depletion removes inhibition to permit the lysine-bearing and voltage-sensing helix to move in response to consequent membrane hyperpolarization. Diacylglycerol is found at interprotomer interfaces, suggesting a role in metabolic control.

11.
J Cell Physiol ; 2019 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-30680718

RESUMO

Hypoxia is a common biological hallmark of solid cancers, which has been proposed to be associated with oncogenesis and chemotherapy resistance. The purpose of the present study was to investigate the role and underlying mechanisms of olfactomedin 4 (OLFM4) in the hypoxia-induced invasion, epithelial-mesenchymal transition (EMT), and chemotherapy resistance of non-small-cell lung cancer (NSCLC). We observed dramatically upregulated expression of OLFM4 in several NSCLC cell lines, and this effect was more pronounced in A549 and H1299 cells. In addition, our data revealed that OLFM4 expression was remarkably increased in both A549 and H1299 cells under hypoxic microenvironment, accompanied by enhanced levels of hypoxia-inducible factor (HIF)-1α protein. The HIF-1α level was elevated in response to hypoxia, resulting in the regulation of OLFM4. Interestingly, OLFM4 was a positive regulator of hypoxia-driven HIF-1α production. Moreover, depletion of OLFM4 modulated multiple EMT-associated proteins, as evidenced by the enhanced E-cadherin levels along with the diminished expression of N-cadherin and vimentin in response to hypoxia, and thus blocked invasion ability of A549 and H1299 cells following exposure to hypoxia. Furthermore, ablation of OLFM4 accelerated the sensitivity of A549 cells to cisplatin under hypoxic conditions, implying that OLFM4 serves as a key regulator in chemotherapeutic resistance under hypoxia. In conclusion, OLFM4/HIF-1α axis might be a potential therapeutic strategy for NSCLC.

12.
J Cancer ; 9(18): 3352-3360, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30271496

RESUMO

Purpose: Investigating surrogate endpoints shortening the time of therapeutic evaluation in nasopharyngeal carcinoma (NPC) after radical treatment. Patients and Methods: We retrospectively analyzed 830 patients receiving intensity-modulated radiotherapy (IMRT) from 2008 to 2010 and being stratified by the 8th edition of UICC/AJCC staging system and the plasma Epstein-Barr virus DNA (EBV DNA). The annual rates of overall survival (OS), progression-free survival (PFS), loco-regional recurrence-free survival (LRFS), and distant metastasis-free survival (DMFS) were sequentially calculated using the life table and compared by the McNemar method. Results: The time of shortening therapeutic evaluation by surrogate endpoints: OS, PFS, LRFS and DMFS could be shortened to 1-year (100% vs 100%, P=1) in patients with stage I; OS, PFS, LRFS and DMFS could be shortened to 3-year (96.9% vs 96.1%, P = 1; 94.6% vs 92.2%, P = 0.125; 96.9% vs 95.3%, P = 0.5) and 4-year (92.2% vs 91.2%, P = 0.125) in stage II; In the high EBV DNA group , OS and DMFS could be shortened to 1-year (100% vs 100%, P = 1;100% vs 100%, P = 0.25) in stage II; OS and PFS could be shortened to 3-year (94.3% vs 91.4%, P = 1;82.9% vs 74.3%, P = 0.25) in stage III; OS could be shortened to 4-year (75% vs 72.7%, P = 1) in stage IVA. Conclusions: The time of therapeutic evaluation could be shortened to <5-year in stages I-II patients. The year of surrogate endpoints could be ahead in stages II-IVA with high EBV DNA.

13.
Brain Res ; 2018 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-30321496

RESUMO

BACKGROUND: MYCN and LMO1 amplification are commonly observed in neuroblastoma (NB), which was often accompanied by genetic loss of let-7 microRNA (miRNA). Fibroblast growth factor (FGF) was found to regulate let-7 miRNA expression via FGF receptor substrate 2 (FRS2), which then activates transforming growth factor beta (TGF-ß) signaling. METHODS: Expression of MYCN, LMO1, FRS2, let-7, and TGF-ß receptor I (TGFßRI) was selectively knocked-down or enhanced in NB cells. Proliferation, invasion, migration, metastasis and tumorigenesis of NB, expression of downstream signaling factors and metastasis-associated protein were evaluated. RESULTS: Knock-down on either MYCN or LMO1 has led to inhibition on proliferation, invasion, migration, and metastasis of NB cells, and knock-down of FRS2 resulted in increases in MYCN and LMO1 expression and enhanced invasion, migration and metastasis of NB cells. Decreased expression of TGF-ß1 or TGFßRI led to decrease expression in LMO1 and proliferation, invasion, migration and metastasis markers, except MYCN expression which appeared not to be regulated by TGF-ß1 or TGFßRI. Furthermore, let-7 miRNA was shown to decrease the expression levels of TGF-ßRI, LMO1 and MYCN. CONCLUSIONS: FGF regulates MYCN and TGF-ß1-induced LMO1 and metastasis of NB cells via let-7 miRNA.

14.
Zhongguo Zhong Yao Za Zhi ; 43(15): 3205-3210, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200719

RESUMO

In recent years,hepatotoxicity problem of Polygonum multiflorum has caused high attention. Domestic scholars also explored the causes of liver damage caused by it. For example, the establishment of guideline for diagnosis and treatment of herb-induced liver injury, and the theory about relationship between hepatocyte toxicity and chemical composition, solvents, processing, use and pathological basis of patients and so on. To try to combine theory with practice,author analyzed risk factors about the case reports of P. multiflorum causing liver damage, and made some suggestions on P. multiflorum about individualized application, drug selection and requirements for taking. This for providing reference for the safe use of P. multiflorum.

15.
Huan Jing Ke Xue ; 39(10): 4809-4816, 2018 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-30229631

RESUMO

In order to investigate the effect of multiple fluoroquinolones on the performance and microbial community of a constructed wetland, ofloxacin, norfloxacin, and ciprofloxacin were added to the influent of the vertical flow constructed wetland for two months. Results indicated that COD removal rate gradually decreased after adding antibiotics, with the minimum removal rate of 70.94%, followed by gradual recovery. TP removal rate also decreased, with some fluctuations, while ammonia removal remained stable. Fluoroquinolones thus have an important effect on COD and TP removal from the constructed wetland, but there is no evident effect on ammonia removal. Based on results of the Shannon index and Shannoneven index, there were no significant changes in the microbial community, while the Chao1 index increasing significantly. Comparing community composition before and after antibiotic addition, the relative abundance of Proteobacteria decreased from 44.90% to 34.12%, still maintaining its predominance, while Firmicutes increased from 2.55% to 10.55%. At the class level, ß-Proteobacteria declined from 17.03% to 8.36%, while the relative abundance of Clostridia, Bacilli, and Bacteroidia increased from 0.50%, 1.85%, and 0.10% to 4.21%, 4.64%, and 2.56%, respectively. The genera Dechloromonas and Pseudarthrobacter decreased from 8.56% and 5.10% to 3.16% and 1.53%, respectively, while Trichococcus, Tessaracoccus, and Desulfovibrio increased from 0.66%, 0.03%, and 0.02% to 3.84%, 3.83%, and 2.06%, respectively. The microbial community of the constructed wetland thus changed under the pressure of multiple fluoroquinolones.

16.
Sci Rep ; 8(1): 11081, 2018 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-30038348

RESUMO

We investigate the behavior of coherence in scattering quantum walk search on complete graph under the condition that the total number of vertices of the graph is significantly larger than the marked number of vertices we are searching, N ≫ v. We find that the consumption of coherence represents the increase of the success probability for the searching, also it is related to the efficiency of the algorithm in oracle queries. If no coherence is consumed or an incoherent state is utilized, the algorithm will behave as the classical blind search, implying that coherence is responsible for the speed-up in this quantum algorithm over its classical counterpart. The effect of noises, in particular of photon loss and random phase shifts, on the performance of algorithm is studied. Two types of noise are considered because they arise in the optical network used for experimental realization of scattering quantum walk. It is found that photon loss will reduce the coherence and random phase shifts will hinder the interference between the edge states, both leading to lower success probability compared with the noise-free case. We then conclude that coherence plays an essential role and is responsible for the speed-up in this quantum algorithm.

17.
Chin J Nat Med ; 16(6): 446-455, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30047466

RESUMO

Saposhnikovia divaricata is a valuable Chinese medicinal herb; the transformation from vegetative growth to reproductive growth may lead to the decrease of its pharmacological activities. Therefore, the study of bolting and flowering for Saposhnikovia divaricata is warranted. The present study aimed to reveal differentially expressed genes (DEGs) and regularity of expression during the bolting and flowering process, and the results of this study might provide a theoretical foundation for the suppression of early bolting for future research and practical application. Three sample groups, early flowering, flower bud differentiation, and late flowering (groups A, B, and C, respectively) were selected. Transcriptomic analysis identified 67, 010 annotated unigenes, among which 50, 165 were differentially expressed including 16, 108 in A vs B, and 17, 459 in B vs C, respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway functional classification analysis were performed on these differentially expressed genes, and five important pathways were significantly impacted (P ≤ 0.01): plant circadian rhythm, other glycan degradation, oxidative phosphorylation, plant hormone signal transduction, and starch and sucrose metabolism. Plant hormone signal transduction might play an important role in the bolting and flowering process. The differentially expressed indole-3-acetic acid (IAA) gene showed significant down-regulation during bolting and flowering, while the transport inhibitor response 1 (TIR1) gene showed no significant change during the bolting process. The expression of flowering related genes FLC, LYF, and AP1 also showed a greater difference at different development stages. In conclusion, we speculate that the decrease in auxin concentration is not caused by the degrading effect of TIR1 but by an alternative mechanism.


Assuntos
Apiaceae/crescimento & desenvolvimento , Apiaceae/genética , Flores/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Flores/crescimento & desenvolvimento , Redes Reguladoras de Genes , RNA de Plantas/genética , Reprodutibilidade dos Testes
18.
Sheng Li Xue Bao ; 70(3): 319-328, 2018 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-29926074

RESUMO

Recently, there has been a growing interest in the roles of androgen in the development of obesity and obesity-related diseases such as diabetes, metabolic syndrome, atherosclerosis (AS), hypertension and cardiovascular diseases, as well as in the disorders of glucose and lipid metabolism. Androgen exerts its biological role mainly through binding to the androgen receptor (AR), one of the nuclear receptors widely expressed in multiple tissues such as the skeletal muscle, liver, adipose tissue and brain. Low testosterone and AR deficiency result in obesity, obesity-related diseases and metabolic disorders. Androgen regulates almost all the intracellular transduction pathways related to glucose and lipid metabolism, including key metabolic enzymes/proteins, nuclear transcription factors (PPARγ, LXRα and FoxO1), inflammation, leptin sensitivity of hypothalamus, proliferation and differentiation of adipocyte, mitochondrial function and vascular endothelial function. In addition, females with high androgen are more likely to develop obesity as well as glucose and lipid metabolism disorders, while the underlying mechanisms remain unclear. This review focuses on the role and underlying mechanisms of androgen and AR in the development of obesity and obesity-related diseases as well as glucose and lipid metabolism disturbance in men.

19.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 34(1): 78-82, 2018 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-29926665

RESUMO

OBJECTIVE: To investigate the effects of 4-week moderate aerobic exercise plus diet control on serum levels of total insulin-like growth factor 1(IGF-1) and IGF-1 binding protein-3 (IGFBP-3) as well as IGF-1 activity (reflected by molar ratio of IGF-1/IGFBP-3) in female obese adolescents and youths, and their possible role on fat loss, and improvement of glucose and lipid metabolism. METHODS: Nine female obese youths (age:18~19 y) and 30 female obese adolescents (age:14~16 y) were recruited and undertook 4-week aerobic exercise such as swimming and jogging (6 days/week, twice a day, 2 h/time with 5 min rest per 30 min exercise) with gradual increase of intensity from low (heart rate immediately post-exercise of 1st week:100~120 beats/min) to moderate (heart rate immediately post-exercise of 2nd-4th weeks:120~140 beats/min) level, combined with a diet intervention (total daily energy intake of 1 400 or 1 600 kcal according to basal metabolism rate) in Shanghai Dianfeng weight loss enclosed camp. Nine normal weight young women and 9 female children matched at age and nationality were recruited as the normal control. Before and after the experimental period, anthropometric index (body weight, body mass index(BMI) and waist circumference), glucose and lipid metabolism parameters including fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), triglyceride (TG); total cholesterol (TC), low density lipoprotein (LDL) and high density lipoprotein (HDL), and serum levels of total IGF-1 and IGFBP-3 were measured, and IGF-1 activity was calculated in the obese and normal control female adolescents. RESULTS: ①Compared with normal control, the serum levels of total IGF-1 and IGFBP-3 were decreased in the female obese youths and adolescents, and IGF-1 activity was reduced only in the obese female adolescents. ②The serum level of IGFBP-3 was down-regulated and IGF-1 activity was up-regulated while no change of serum total IGF-1 was induced by 4-week moderate aerobic exercise plus diet control, accompanied with significant decreases of body weight, BMI and waist circumference as well as improvement of glucose and lipid metabolism in the female obese youths and adolescents. Except for a positive association between the increased IGF-1 activity and the decreased waist circumference was found in the female obese youths by Pearson's correlation analysis, there was no relation of the decreased IGFBP-3, the increased IGF-1 activity with the improvements of anthropometric index and glucose and lipid metabolism in female obese youths and adolescents. CONCLUSIONS: The serum level of IGFBP-3 was down-regulated and the IGF-1 activity was up-regulated by 4-week moderate aerobic exercise plus diet control in female obese youths and adolescents. The increase of IGF-1 activity might be associated with the exercise-plus-diet-induced decrease of waist circumstance in female obese youths.

20.
Clin Neurol Neurosurg ; 170: 43-46, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29727800

RESUMO

OBJECTIVE: The aim of this single-institution cohort study is to describe clinical characteristics of patients with breast cancer brain metastases (BCBM), to investigate survival after diagnosis of brain metastases (BM), and to assess the aggressive treatments to BCBM. PATIENTS AND METHODS: We identified 134 consecutive patients diagnosed with operable breast cancer and then who developed BM at the Sun Yat-sen University Cancer Center from 2000 to 2015, and analyzed the therapeutic methods for primary breast cancer and BM to evaluate whether they were associated with longer survival after the development of BM. The median age at breast cancer diagnosis was 47 years (range 21-73 years). RESULTS: The median survival after BM was 16.2 months (range 12.1-20.3 months), and the survival rates were 62% and 37% at 1 and 2 years, respectively. Multivariate analysis showed that craniotomy (p = 0.034) and targeted therapy (p < 0.001) for BCBM were positively correlated with survival after diagnosis of BM; radiotherapy (p = 0.024) after surgery for primary breast cancer was beneficial to BM. CONCLUSIONS: Surgical resection and targeted therapy are effective treatment for BCBM. Radiotherapy after surgery for the management of primary breast cancer is necessary in patients with brain progression later.

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