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1.
Artigo em Inglês | MEDLINE | ID: mdl-31671247

RESUMO

Fluorescent materials exhibiting two-photon induction (TPI) are used for nonlinear optics, bioimaging, and phototherapy. Polymerizations of molecular chromophores to form π-conjugated structures were hindered by the lack of long-range ordering in the structure and strong π-π stacking between the chromophores. Reported here is the rational design of a benzothiadiazole-based covalent organic framework (COF) for promoting TPI and obtaining efficient two-photon induced fluorescence emissions. Characterization and spectroscopic data revealed that the enhancement in TPI performance is attributed to the donor-π-acceptor-π-donor configuration and regular intervals of the chromophores, the large π-conjugation domain, and the long-range order of COF crystals. The crystalline structure of TPI-COF attenuates the π-π stacking interactions between the layers, and overcomes aggregation-caused emission quenching of the chromophores for improving near-infrared two-photon induced fluorescence imaging.

2.
ACS Nano ; 13(12): 14230-14240, 2019 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-31714733

RESUMO

Tumorous vasculature plays key roles in sustaining tumor growth. Vascular disruption is accompanied by internal coagulation along with platelet recruitment and the resulting suppression of oxygen supply. We intend to artificially create this physiological process to establish the mutual feedback between vascular disruption and platelet-mimicking biotaxis for the cascade amplification of hypoxia-dependent therapy. To prove this concept, mesoporous silica nanoparticles are co-loaded with a hypoxia-activated prodrug (HAP) and a vessel-disruptive agent and then coated with platelet membranes. Upon entering into tumors, our nanotherapeutic can disrupt local vasculature for tumor inhibition. This platelet membrane-coated nanoplatform shares the hemorrhage-tropic function with parental platelets and can be persistently recruited by the vasculature-disrupted tumors. In this way, the intratumoral vascular disruption and tumor targeting are biologically interdependent and mutually reinforced. Relying on this mutual feedback, tumorous hypoxia was largely promoted by more than 20-fold, accounting for the effective recovery of the HAP's cytotoxicity. Consequently, our bioinspired nanodesign has demonstrated highly specific and effective antitumor potency via the biologically driven cooperation among intratumoral vascular disruption, platelet-mimicking biotaxis, cascade hypoxia amplification, and hypoxia-sensitive chemotherapy. This study offers a paradigm of correlating the therapeutic design with the physiologically occurring events to achieve better therapy performance.

3.
Chin Med Sci J ; 34(3): 168-176, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31601299

RESUMO

Objective To explore circulating biomarkers for screening the invasiveness of non-functioning pituitary adenomas (NF-PAs). Methods The exosomal RNAs were extracted from serum of patients with invasive NF-PA (INF-PA) or noninvasive NF-PA (NNF-PA). Droplet digital PCR was adapted to detect the mRNA expression of candidate genes related to tumor progression or invasion, such as cyclin dependent kinase 6 (CDK6), ras homolog family member U (RHOU), and spire type actin nucleation factor 2 (SPIRE2). Student's t-test was used to analyze the statistical difference in the mRNA expression of candidate genes between the two groups. Receiver operating characteristic (ROC) curve was used to establish a model for predicting the invasiveness of NF-PAs. The accuracy, sensitivity, specificity and precision of the model were then obtained to evaluate the diagnostic performance. Results CDK6 (0.2600±0.0912 vs. 0.1789±0.0628, t=3.431, P=0.0013) and RHOU mRNA expressions (0.2696±0.1118 vs. 0.1788±0.0857, t=2.946, P=0.0052) were upregulated in INF-PAs patients' serum exosomes as compared to NNF-PAs. For CDK6, the area under the ROC curve (AUC) was 0.772 (95% CI: 0.600-0.943, P=0.005), the accuracy, sensitivity, specificity and precision were 77.27%, 83.33%, 75.00% and 55.56% to predict the invasiveness of NF-PAs. For RHOU, the AUC was 0.757 (95% CI: 0.599-0.915, P=0.007), the accuracy, sensitivity, specificity and precision were 72.73%, 83.33%, 68.75% and 50.00%. In addition, the mRNA levels of CDK6 and RHOU in serum exosomes were significantly positively correlated (r=0.935, P<0.001). After combination of the cut-off scores of the two genes, the accuracy, sensitivity, specificity and precision were 81.82%, 83.33%, 81.25% and 62.50%. Conclusions CDK6 and RHOU mRNA in serum exosomes can be used as markers for predicting invasiveness of NF-PAs. Combination of the two genes performs better in distinguishing INF-PAs from NNF-PAs. These results indicate CDK6 and RHOU play important roles in the invasiveness of NF-PAs, and the established diagnostic method is valuable for directing the clinical screening and postoperative treatment.


Assuntos
Adenoma/sangue , Biomarcadores Tumorais/sangue , Quinase 6 Dependente de Ciclina/sangue , Exossomos/metabolismo , Proteínas de Neoplasias/sangue , Neoplasias Hipofisárias/sangue , RNA Mensageiro/sangue , RNA Neoplásico/sangue , Proteínas rho de Ligação ao GTP/sangue , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
4.
J Nutr ; 149(12): 2110-2119, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31504723

RESUMO

BACKGROUND: Selenium is prioritized to the brain mainly for selenoprotein expression. Selenoprotein T (SELENOT) protects dopaminergic, postmitotic neurons in a mouse model of Parkinson's disease (PD). OBJECTIVE: We hypothesized a proliferative role of SELENOT in neural cells. METHODS: To assess SELENOT status in PD, sedated male C57BL/6 mice at 10-12 wk of age were injected with 6-hydroxydopamine in neurons, and human peripheral blood mononuclear cells were isolated from 9 healthy subjects (56% men, 68-y-old) and 11 subjects with PD (64% men, 63-y-old). Dopaminergic neural progenitor-like SK-N-SH cells with transient SELENOT overexpression or knockdown were maintained in the presence or absence of the antioxidant N-acetyl-l-cysteine and the calcium channel blocker nimodipine. Cell cycle, proliferation, and signaling parameters were determined by immunoblotting, qPCR, and flow cytometry. RESULTS: SELENOT mRNA abundance was increased (P < 0.05) in SK-N-SH cells treated with 1-methyl-4-phenylpyridinium iodide (3.5-fold) and peripheral blood mononuclear cells from PD patients (1.6-fold). Likewise, SELENOT was expressed in tyrosine hydroxylase-positive dopaminergic neurons of 6-hydroxydopamine-injected mice. Knockdown of SELENOT in SK-N-SH cells suppressed (54%; P < 0.05) 5-ethynyl-2'-deoxyuridine incorporation but induced (17-47%; P < 0.05) annexin V-positive cells, CASPASE-3 cleavage, and G1/S cell cycle arrest. SELENOT knockdown and overexpression increased (88-120%; P < 0.05) and reduced (37-42%; P < 0.05) both forkhead box O3 and p27, but reduced (51%; P < 0.05) and increased (1.2-fold; P < 0.05) cyclin-dependent kinase 4 protein abundance, respectively. These protein changes were diminished by nimodipine or N-acetyl-l-cysteine treatment (24 h) at steady-state levels. While the N-acetyl-l-cysteine treatment did not influence the reduction in the amount of calcium (13%; P < 0.05) by SELENOT knockdown, the nimodipine treatment reversed the decreased amount of reactive oxygen species (33%; P < 0.05) by SELENOT overexpression. CONCLUSIONS: These cellular and mouse data link SELENOT to neural proliferation, expanding our understanding of selenium protection in PD.

5.
Insects ; 10(8)2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31443161

RESUMO

The morphology and distribution of the antennal sensilla of Megalurothrips usitatus Bagnall and Thrips palmi Karny were examined using scanning electron microscopy (SEM). These are serious pests of various economically important crops, and their antennae are important in chemical communication. The antennae of both species consist of a scape, pedicel, and flagellum, but the flagellum of M. usitatus is made up of six sub-segments, whereas that of T. palmi consists of five sub-segments. Seven morphological sensilla types, including Böhm bristle (BB), sensilla campaniformia (Sca), three types of sensilla basiconica (Sb1, Sb2 and Sb3), two types of sensilla chaetica (Sch1 and Sch2), sensilla styloconica (Sst), sensilla trichodea (St), and sensilla cavity (Scav), were recorded in both species. The scape and pedicels exhibited Sch1, BB and Sca. The flagellum exhibited two types of Sch, three types of Sb, St, Sst and Scav. Based on these results, the putative function of the sensilla of M. usitatus and T. palmi are also discussed.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31465879

RESUMO

The ATF/CREB family of transcription factors represents a large group of basic region-leucine zipper (bZip) proteins that regulate diverse cellular responses. Here we carried out a comprehensive analysis of ATF/CREB family members in 22 representative animal species. The family probably originated from the early diverging metazoan and significantly expanded in vertebrates due to multiple whole genome duplication. Duplicates of atf6 were derived from 2R, and duplicates of creb1, crem, jdp2, creb5, atf4, atf5 and atf7 were products of 3R. We also isolated 21 ATF/CREBs, belonging to 6 subfamilies from Nile tilapia. Based on transcriptome data, most members were found to be dominantly expressed in the head kidney, heart, brain and testis. Some ATF/CREBs displayed sexual dimorphic expression in gonad at 5, 90 and 180 dah (days after hatching), but not at 30 dah. creb1a and atf4a were found to be expressed mainly in phase I and II oocytes of the ovary; while creb1b and atf4b mainly in spermatogenic cells of the testis, indicating divergence of duplicated genes from 3R which suggested neofunctionalization or subfunctionalization in gonad. This is the first genome-wide screening and evolutionary analysis of ATF/CREB family in different animals, particularly in teleosts. The expression analysis of this family in tilapia gonad provided a fundamental clue for understanding their important roles in sex differentiation and gonadal development in teleosts.


Assuntos
Fatores Ativadores da Transcrição/metabolismo , Proteína de Ligação a CREB/metabolismo , Ciclídeos/metabolismo , Evolução Molecular , Gônadas/metabolismo , Fatores Ativadores da Transcrição/genética , Animais , Proteína de Ligação a CREB/genética , Ciclídeos/genética , Feminino , Perfilação da Expressão Gênica , Masculino , Ovário/metabolismo , Testículo/metabolismo
7.
Angew Chem Int Ed Engl ; 58(17): 5692-5696, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30848514

RESUMO

Polycyclic aromatic derivatives can trap 1 O2 to form endoperoxides (EPOs) for O2 storage and as sources of reactive oxygen species. However, these materials suffer from structural amorphism, which limit both practical applications and fundamental studies on their structural optimization for O2 capture and release. Metal-organic frameworks (MOFs) offer advantages in O2 binding, such as clear structure-performance relationships and precise controllability. Herein, we report the reversible binding of O2 is realized via the chemical transformation between anthracene-based and the corresponding EPO-based MOF. It is shown that anthracene-based MOF, the framework featuring linkers with polycyclic aromatic structure, can rapidly trap 1 O2 to form EPOs and can be restored upon UV irradiation or heating to release O2 . Furthermore, we confirm that photosensitizer-incorporated anthracene-based MOF are promising candidates for reversible O2 carriers controlled by switching Vis/UV irradiation.

8.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 40(3): 308-314, 2018 Jun 28.
Artigo em Chinês | MEDLINE | ID: mdl-29978784

RESUMO

Objective To investigate the role of methyltransferase-like 3(METTL3) in the proliferation of acute myeloid leukemia (AML) cells and its mechanism. Methods METTL3 expression in AML patients was analyzed in Gene Expression Omnibus data files. METTL3 expression was inhibited by lentivirus-mediated gene transduction in MOLM13 cells,after which cell proliferation was analyzed by cell counting kit-8,N6-methyladenosine (m6A) levels of total mRNA was analyzed by ELISA,specific m6A on MYC was analyzed by gene-specific m6A RNA immunoprecipitation,and MYC expression was analyzed by RT-qPCR and Western blot analysis. Results METTL3 level was slightly increased in AML-M5 patients,and its expression was significantly higher in immature cells than in mature monocytes (t=4.504,P=0.0098). METTL3 knock-down significantly suppressed cell proliferation (P<0.001),reduced m6A level of total mRNA (t=3.606,P=0.042) and specific m6A level on MYC mRNA (P<0.01),and suppressed MYC expression (P<0.01). Conclusion METTL3 acts as an oncogene in MOLM13 cells by upregulating MYC expression.


Assuntos
Adenosina/análogos & derivados , Proliferação de Células , Leucemia Mieloide Aguda/metabolismo , Metiltransferases/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Adenosina/metabolismo , Humanos
9.
ACS Nano ; 12(5): 4630-4640, 2018 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-29584395

RESUMO

We report on the benzoporphyrin-based metal-organic framework (TBP-MOF), with 10-connected Zr6 cluster and much improved photophysical properties over the traditional porphyrin-based MOFs. It was found that TBP-MOF exhibited red-shifted absorption bands and strong near-infrared luminescence for bioimaging, whereas the π-extended benzoporphyrin-based linkers of TBP-MOF facilitated 1O2 generation to enhance O2-dependent photodynamic therapy (PDT). It was demonstrated that poly(ethylene glycol)-modified nanoscale TBP-MOF (TBP-nMOF) can be used as an effective PDT agent under hypoxic tumor microenvironment. We also elucidated that the low O2-dependent PDT of TBP-nMOF in combination with αPD-1 checkpoint blockade therapy can not only suppress the growth of primary tumor, but also stimulate an antitumor immune response for inhibiting metastatic tumor growth. We believe this TBP-nMOF has great potential to serve as an efficient photosensitizer for PDT and cancer immunotherapy.


Assuntos
Antineoplásicos/química , Estruturas Metalorgânicas/química , Nanopartículas/química , Metástase Neoplásica/terapia , Fármacos Fotossensibilizantes/química , Porfirinas/química , Zircônio/química , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Antígeno B7-H1/antagonistas & inibidores , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Terapia Combinada , Xenoenxertos , Humanos , Imunoterapia , Estruturas Metalorgânicas/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Polietilenoglicóis/química , Porfirinas/farmacologia , Receptor de Morte Celular Programada 1/antagonistas & inibidores , Oxigênio Singlete/metabolismo , Distribuição Tecidual , Microambiente Tumoral
10.
Small ; 14(14): e1703680, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29488317

RESUMO

Molybdenum ditelluride nanosheets encapsulated in few-layer graphene (MoTe2 /FLG) are synthesized by a simple heating method using Te and Mo powder and subsequent ball milling with graphite. The as-prepared MoTe2 /FLG nanocomposites as anode materials for lithium-ion batteries exhibit excellent electrochemical performance with a highly reversible capacity of 596.5 mAh g-1 at 100 mA g-1 , a high rate capability (334.5 mAh g-1 at 2 A g-1 ), and superior cycling stability (capacity retention of 99.5% over 400 cycles at 0.5 A g-1 ). Ex situ X-ray diffraction and transmission electron microscopy are used to explore the lithium storage mechanism of MoTe2 . Moreover, the electrochemical performance of a MoTe2 /FLG//0.35Li2 MnO3 ·0.65LiMn0.5 Ni0.5 O2 full cell is investigated, which displays a reversible capacity of 499 mAh g-1 (based on the MoTe2 /FLG mass) at 100 mA g-1 and a capacity retention of 78% over 50 cycles, suggesting the promising application of MoTe2 /FLG for lithium-ion storage. First-principles calculations exhibit that the lowest diffusion barrier (0.18 eV) for lithium ions along pathway III in the MoTe2 layered structure is beneficial for improving the Li intercalation/deintercalation property.

11.
Biomaterials ; 151: 1-12, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29040939

RESUMO

In this report, a biomimetic theranostic oxygen (O2)-meter (cancer cell membrane@Pt(II) porphyrinic-metal organic framework, designated as mPPt) was constructed for cancer targeted and phosphorescence image-guided photodynamic therapy (PDT). mPPt presents high photosensitizers (PSs) loading and evitable self-quenching behaviors for favorable biological O2 sensing and PDT. Besides, endowed by the surface functionalization of cancer cell membrane, the homotypic targeting and immune escape abilities of mPPt could dramatically enhance its cancer targeting ability. Importantly, the O2-dependent phosphorescence responsibility of mPPt could be employed to pre-evaluate the real time O2 level in situ and guide the PDT under light irradiation. A significant anticancer effect is observed after intravenous injection of mPPt and subsequent treatment with PDT with no obvious side effects. As a versatile platform for cell imaging, O2 fluctuation monitoring as well as PDT, this biomimetic O2-meter exhibits great potential for biological analysis and personalized cancer theranostics.


Assuntos
Antineoplásicos/química , Estruturas Metalorgânicas/química , Oxigênio/análise , Fotoquimioterapia/métodos , Nanomedicina Teranóstica/métodos , Animais , Antineoplásicos/farmacologia , Transporte Biológico , Biomimética/métodos , Linhagem Celular Tumoral , Complexos de Coordenação/química , Fluorescência , Haplorrinos , Humanos , Luz , Camundongos , Camundongos Endogâmicos BALB C , Nanopartículas/química , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Imagem Óptica/métodos , Oxigênio/metabolismo , Tamanho da Partícula , Fármacos Fotossensibilizantes/química , Platina/química , Porfirinas/química , Propriedades de Superfície
12.
J Infect Dev Ctries ; 12(9): 794-798, 2018 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-31999639

RESUMO

INTRODUCTION: Neonates are at high risk of nosocomial infections, especially in developing countries. This study aimed to examine the effectiveness of drug-resistant bacteria (DRB) screening in combination with patient barrier precautions in controlling nosocomial infections in neonatal wards. METHODOLOGY: The clinical data of neonates admitted to the Mianyang Central Hospital, Mianyang, China in 2010 and 2012 were retrospectively analyzed. In 2010, DRB screening was conducted using nasal and anal swabs. In 2012, in addition to the DRB screening, patient barrier precautions were implemented. The barrier precautions were lifted if the patients were negative for the DRB screening. Patients with DRB colonization were further isolated to reduce the risk of nosocomial infection. The rate of nosocomial infections in the two years was compared. RESULTS: A total of 1280 neonates in 2010 and 1504 neonates in 2012 were included in the analysis. No significant difference was noticed between the two years in gestational weeks, age, gender, and birth weight. The rate of nosocomial infections was reduced significantly from 2.34% in 2010 to 1.13% in 2012. CONCLUSIONS: DRB screening in combination with the patient barrier precautions may reduce the risk of nosocomial infection in neonates.

13.
Artigo em Inglês | MEDLINE | ID: mdl-29124041

RESUMO

Disturbed homeostasis of gut microbiota has been suggested to be closely associated with 5-fluorouracil (5-Fu) induced mucositis. However, current knowledge of the overall profiles of 5-Fu-disturbed gut microbiota is limited, and so far there is no direct convincing evidence proving the causality between 5-Fu-disturbed microbiota and colonic mucositis. In mice, in agreement with previous reports, 5-Fu resulted in severe colonic mucositis indicated by weight loss, diarrhea, bloody stool, shortened colon, and infiltration of inflammatory cells. It significantly changed the profiles of inflammatory cytokines/chemokines in serum and colon. Adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and VE-Cadherin were increased. While tight junction protein occludin was reduced, however, zonula occludens-1 (ZO-1) and junctional adhesion molecule-A (JAM-A) were increased in colonic tissues of 5-Fu treated mice. Meanwhile, inflammation related signaling pathways including NF-κB and mitogen activated protein kinase (MAPKs) in the colon were activated. Further study disclosed that 5-Fu diminished bacterial community richness and diversity, leading to the relative lower abundance of Firmicutes and decreased Firmicutes/Bacteroidetes (F/B) ratio in feces and cecum contents. 5-Fu also reduced the proportion of Proteobacteria, Tenericutes, Cyanobacteria, and Candidate division TM7, but increased that of Verrucomicrobia and Actinobacteria in feces and/or cecum contents. The fecal transplant from healthy mice prevented body weight loss and colon shortening of 5-Fu treated mice. In addition, the fecal transplant from 5-Fu treated mice reduced body weight and colon length of vancomycin-pretreated mice. Taken together, our study demonstrated that gut microbiota was actively involved in the pathological process of 5-Fu induced intestinal mucositis, suggesting potential attenuation of 5-Fu induced intestinal mucositis by manipulating gut microbiota homeostasis.


Assuntos
Quimiocinas/metabolismo , Citocinas/metabolismo , Fluoruracila/efeitos adversos , Microbioma Gastrointestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Mucosite/metabolismo , Mucosite/microbiologia , Animais , Antígenos CD/metabolismo , Bactérias/classificação , Bactérias/efeitos dos fármacos , Bactérias/genética , Bactérias/metabolismo , Peso Corporal/efeitos dos fármacos , Caderinas/metabolismo , Moléculas de Adesão Celular/metabolismo , Colo/metabolismo , Colo/patologia , Transplante de Microbiota Fecal , Fezes/microbiologia , Imuno-Histoquímica , Inflamação/complicações , Inflamação/patologia , Molécula 1 de Adesão Intercelular/metabolismo , Mucosa Intestinal/patologia , Intestinos/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Mucosite/induzido quimicamente , Mucosite/patologia , NF-kappa B/metabolismo , RNA Ribossômico 16S/genética , Receptores de Superfície Celular/metabolismo , Molécula 1 de Adesão de Célula Vascular/metabolismo , Proteína da Zônula de Oclusão-1/metabolismo
14.
ACS Appl Mater Interfaces ; 9(49): 43143-43153, 2017 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-29168377

RESUMO

A universal strategy was reported that enables functional group-capped nanostructures with various morphologies and compositions to be coated by porphyrin metal-organic framework (MOF). Based on the nanostructure-induced heterogeneous nucleation, the controlled growth of MOF shell on the surface of nanostructures can be realized. It was demonstrated that this modification strategy can realize controlled growth of porphyrin MOF on a series of organic and inorganic nanostructures, such as polydopamine (PDA) nanoparticles, PDA@Pt nanoparticles, graphene oxide sheets, and Au nanorods. The as-prepared composites exhibit excellent catalytic and optical properties that originate from the nanostructure as well as the coated porphyrin MOF. We further explored the potential applications of PDA@MOF and PDA@Pt@MOF in nanomedicine and photocatalysis.

15.
Oncotarget ; 8(33): 55453-55466, 2017 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-28903433

RESUMO

Myelopoiesis is under the control of a complex network containing various regulation factors. Deregulation of any important regulation factors may result in serious consequences including acute myeloid leukemia (AML). In order to find out the genes that may take a part in AML development, we analyzed data from AML cDNA microarray (GSE2191) in the NCBI data pool and noticed that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is abnormally over-expressed in AML patients. Then we investigated the function and mechanisms of hnRNP A1 in myeloid development. A gradually decreased hnRNP A1 expression was detected during granulocytic differentiation in ATRA-induced-NB4 and HL-60 cells and cytokines-induced hematopoietic stem and progenitor cells. By function-loss and winning experiments we demonstrated hnRNP A1's inhibition role via inhibiting expression of C/EBPα, a key regulator of granulocytic differentiation, in the granulocytic differentiation. During granulocytic differentiation the decrease of hnRNP A1 reduces inhibition on C/EBPα expression, and the increased C/EBPα promotes the differentiation. We also demonstrated that miR-451 promotes granulocytic differentiation via targeting to and down-regulating hnRNP A1, and hnRNP A1 positively regulates c-Myc expression. Summarily, our results revealed new function and mechanisms of hnRNP A1 in normal granulocytiesis and the involvement of a feed-back loop comprising c-Myc, miR-451 and hnRNP A1 in AML development.

16.
Sci Rep ; 6: 19291, 2016 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-26765747

RESUMO

A previous study demonstrated that birds that are exposed to light at night develop advanced reproductive systems. However, spectrum might also affect the photoperiodic response of birds. The present study was aimed to investigate the effects of spectral composition on the growth and reproductive physiology of female breeders, using pure light-emitting diode spectra. A total of 1,000 newly hatched female avian breeders (Gallus gallus) were equally allocated to white-, red-, yellow-, green- and blue-light treated groups. We found that blue-light treated birds had a greater and faster weight gain than did red- and yellow-light treated birds (P = 0.02 and 0.05). The red light expedited the sexual maturation of the chicks, whose age at sexual maturity was 7 and 14 days earlier than that of the green- and blue-light treated birds, respectively. The accumulative egg production of the red-light treated birds was 9 and 8 eggs more than that of the blue- and green-light treated birds. The peak lay rate of the red-light treated groups was significantly greater than the blue-light treated birds (P = 0.028). In conclusion, exposure to short-wavelength light appears to promote growth of female breeder birds, whereas exposure to long-wavelength light appears to accelerate reproductive performance.


Assuntos
Galinhas/fisiologia , Luz , Fotoperíodo , Reprodução , Animais , Peso Corporal , Galinhas/crescimento & desenvolvimento , Feminino , Fertilidade , Maturidade Sexual
17.
Zhongguo Zhong Yao Za Zhi ; 41(8): 1498-1503, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-28884546

RESUMO

To investigate the inhibitory effect and mechanism of vina-ginsenoside R7 (R7) on the activation of rat C6 astrocytes cells induced by LPS/TNF-α, cells in logarithmic growth phase were cultured in DMEM medium without FBS for 24 h. After dissociated using 0.25% EDTA-trypsin, the cells were seeded into respective plates at the density of 1.5×106 cells per mL and cultured overnight. The cells were divided into the following groups: control group (no treatment), model group (treated with LPS 1 µg•mL⁻¹ and TNF-α 10 µg•L⁻¹ treated for 24 h), R7 groups (pre-treated with 6.25, 12.5, 25, 50, and 75 µmol•L⁻¹ R7, 4 µmol•L⁻¹ L-NMMA for 2 h and then stimulated with LPS 1 mg•L⁻¹ and TNF-α 10 µg•L⁻¹ for 24 h). Cell viability was analyzed by CCK-8 kit. Secretion of nitric oxide (NO) in the medium was measured by Greiss method. Concentrations of interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) were assayed by ELISA kits. Gene expressions of inflammatory factors were examined by quantitative-PCR analysis. Activation of NF-κB was detected by dual luciferase reporter gene assay kit. The results showed that R7 could significantly inhibit the secretion of NO from C6 cells in a dose-effect manner, with an IC50 of 34 µmol•L⁻¹. And it could reduce cell proliferation induced by LPS/TNF-α stimulation. Furthermore, R7 at 50 µmol•L⁻¹ significantly down-regulated gene expressions of iNOS (P<0.001), TNF-α (P<0.001), IL-1ß(P<0.05), and COX-2 (P<0.001), but could not change gene expression of IL-6. However, R7 reduced the secretion of TNF-α (P<0.001) and IL-6 (P<0.001). Further studies disclosed that, different concentrations of R7 (25, 50, 100 µmol•L⁻¹) could significantly inhibit the transcription activity of NF-κB(P<0.05, P<0.01, and P<0.001). In conclusion, R7 could inhibit inflammatory responses in C6 cells induced by LPS/TNF-α probably by inhibiting the transcription activity of NF-κB, which indicates its possible therapeutic effect in neurological diseases related to neuroinflammation.


Assuntos
Astrócitos/efeitos dos fármacos , Ginsenosídeos/farmacologia , Animais , Células Cultivadas , Regulação para Baixo , Inflamação , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , NF-kappa B/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Ratos , Fator de Necrose Tumoral alfa/farmacologia
18.
Oncol Lett ; 10(3): 1444-1446, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26622687

RESUMO

Giant cystic pheochromocytomas (GPCCs) are rare adrenal tumors and the majority of them present as asymptomatic. As a result GPCCs often remain undiagnosed until surgery and therefore the surgical team face a greater challenge in perioperative management. The present study describes the case of a 36 year-old woman with an undiagnosed GPCC, which was successfully resected despite the occurrence of perioperative cardiovascular events, including hypertension, hypotension, ventricular arrhythmias, acute heart failure, acute myocardial infarction, and the patient was discharged home without any recurrence. It should be considered in retroperitoneal tumour of patients with nonspecific symptoms and given adequate treatment to promote the perioperative safety.

19.
Mol Med Rep ; 12(5): 7039-44, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26299324

RESUMO

Surgical procedures cause a decrease in lymphocyte proliferation rate, an increase in apoptosis and shifts the balance of T­helper (Th)1/Th2 cells towards anti­cell­mediated immunity (CMI) Th2 dominance, which is relevant to the immunosuppressive effects of CMI, postoperative septic complications and the formation of tumor metastasis. Previous studies have revealed that lidocaine exhibits antibacterial actions; regulating inflammatory responses, reducing postoperative pain and affecting the duration spent in hospital. Thus, the present study hypothesized that lidocaine may exert a protective effect on the CMI of patients undergoing surgery for the removal of a primary tumor. A total of 30 adult female patients diagnosed with cervical cancer were recruited to the present study and were randomized into two groups. The lidocaine group received an intravenous bolus dose of 1.5 mg/kg lidocaine, followed by continuous infusion at 1.5 mg/kg/h until discharge from the operating room. The control group received the same volume of normal saline. A 10 ml sample of venous blood was drawn, and the lymphocytes were isolated using Ficoll­paque 1 day prior to surgery, at discharge from the operating room and 48 h post­surgery. The proliferation rate of the lymphocytes was assessed using a Cell Counting Kit­8 assay and was found to be higher in the lidocaine group. The early apoptosis of lymphocytes was attenuated following lidocaine treatment at 48 h post­surgery, as detected using flow cytometry with Annexin V­fluorescein isothiocyanate/propidium iodide staining. The level of interferon (IFN)­Î³ in the serum at 48 h was significantly decreased following surgery in the control group, compared with the pre­surgical values (3.782 ± 0.282, vs. 4.089 ± 0.339 pg/ml, respectively) and the ratio of IFN­Î³ to interleukin­4 was well preserved in the lidocaine group. In conclusion, the present study demonstrated that the intraoperative systemic administration of lidocaine exerted a protective effect on CMI in patients with cervical cancer undergoing radical hysterectomy. This may be beneficial in reducing the occurrence of postoperative septic complications and tumor metastasis formation.


Assuntos
Anestésicos Locais/administração & dosagem , Imunidade Celular/efeitos dos fármacos , Lidocaína/administração & dosagem , Neoplasias do Colo do Útero/cirurgia , Adulto , Idoso , Anestésicos Locais/farmacologia , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Feminino , Proteína HMGB1/sangue , Humanos , Histerectomia , Injeções Intravenosas , Interferon gama/sangue , Interleucina-4/sangue , Lidocaína/farmacologia , Linfócitos/citologia , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismo , Pessoa de Meia-Idade , Dor Pós-Operatória/prevenção & controle , Estudos Prospectivos , Resultado do Tratamento , Neoplasias do Colo do Útero/tratamento farmacológico , Neoplasias do Colo do Útero/patologia
20.
Chem Commun (Camb) ; 51(65): 12970-3, 2015 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-26176026

RESUMO

In this study, a novel UV-responsive drug carrier based on mesoporous silica nanoparticles (MSNs) has been designed. A 'self' peptide was introduced onto the MSN surface through a supramolecular photoswitchable heteroternary complexation of cucurbit[8]uril, azobenzene and 4,4'-dipyridyl derivative. The light-induced isomerization of azobenzene contributed to the formation of intelligent cucurbit[8]uril based self-preserving MSNs (CSMSNs). In vitro studies demonstrated that the CSMSNs were effectively shielded against macrophages by the peptide protective layer. Doxorubicin hydrochloride (DOX·HCl) could be well encapsulated in the CSMSNs and exhibit low toxicity without UV-irradiation. After reaching the tumor tissue, the peptide camouflage was removed upon UV-irradiation, which enabled the target ligand mediated cell uptake of the drug carrier and the accelerated drug release.


Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Hidrocarbonetos Aromáticos com Pontes/química , Preparações de Ação Retardada/química , Doxorrubicina/administração & dosagem , Imidazóis/química , Peptídeos/química , Dióxido de Silício/química , Antibióticos Antineoplásicos/farmacocinética , Compostos Azo/química , Linhagem Celular Tumoral , Doxorrubicina/farmacocinética , Humanos , Porosidade , Piridinas/química , Raios Ultravioleta
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