Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 308
Filtrar
2.
Artigo em Inglês | MEDLINE | ID: mdl-31518383

RESUMO

Pancreatic cancer (PC) is the seventh most frequent cause of cancer-related deaths worldwide with a high mortality. MicroRNAs (miRNAs) act as important regulators for the development of PC and participate in the progression of PC. miR-129-5p was reported to regulate the progression of tumors, such as thyroid cancer and gastric cancer. However, the function of miR-129-5p in PC is still unclear. In this study, the down-regulation of miR-129-5p was detected in PC tissues and PC cells. miR-129-5p was overexpressed or knocked down in AsPC-1 and BxPC-3 cells. The results showed that miR-129-5p overexpression suppressed proliferation, migration and invasion, and induced apoptosis of PC cells, whereas miR-129-5p knockdown showed opposite effects. In addition, we found that pre-B-cell leukemia homeobox 3 (PBX3) overexpression promoted proliferation, migration and invasion, but reduced apoptosis of PC cells. PBX3 was identified as a target of miR-129-5p by informatics analysis and dual luciferase reporter assay. Finally, our results indicated that miR-129-5p suppressed cell proliferation and migration by targeting PBX3. This study demonstrated that miR-129-5p could function as a tumor suppressor in the progression and development of PC by targeting PBX3, providing a reliable prognostic factor and a new therapeutic strategy for PC.

3.
Plant Mol Biol ; 101(3): 315-323, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31392474

RESUMO

KEY MESSAGE: Pre-treatment of soybean seedlings with 200 µM salicylic acid before fungal inoculation significantly alleviated disease resistance in soybean seedlings against Fusarium solani infection. Sudden death syndrome of soybean is largely caused by Fusarium solani (F. solani). Salicylic acid (SA) has been reported to induce resistance in plants against many pathogens. However, the effect of exogenous SA application on F. solani infection of soybean is less reported. This study investigated the effect of foliar application of SA on soybean seedlings before F. solani infection. Seedlings were sprayed with 200 µM SA and inoculated with F. solani after 24 h of last SA application. After 3 days post-inoculation, seedlings treated with 200 µM SA showed significantly fewer disease symptoms with increased endogenous SA level, SA marker genes expression and antioxidant activities in the SA-treated seedlings more than the untreated control seedlings. Furthermore, the decrease in hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels was observed in the SA-treated plants as compared to the untreated plants. Analysis of the effect of SA application on F. solani showed that the mycelia growth of F. solani was not affected by SA treatment. Further investigation in this study revealed a decreased in F. solani biomass content in the SA treated seedlings. Results from the present study show that pre-treatment of 200 µM SA can induce resistance of soybean seedlings against F. solani infection.


Assuntos
Resistência à Doença/efeitos dos fármacos , Fusarium/patogenicidade , Doenças das Plantas/microbiologia , Ácido Salicílico/farmacologia , Soja/microbiologia , Regulação da Expressão Gênica de Plantas , Peróxido de Hidrogênio/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/microbiologia , Plântula/efeitos dos fármacos , Plântula/microbiologia , Soja/efeitos dos fármacos
4.
Clin Chim Acta ; 497: 114-119, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31344366

RESUMO

BACKGROUND: Klotho is an anti-aging protein and its increased plasma concentrations were related to good functional outcome of acute ischemic stroke. This study was designed to ascertain the prognostic significance of plasma Klotho in intracerebral hemorrhage. METHODS: Plasma Klotho concentrations in 96 intracerebral hemorrhage patients and 96 healthy controls were quantified. Poor prognosis was defined as modified Rankin scale scores >2 at 90 days. The association of plasma Klotho concentrations with stroke prognosis was assessed using regression model. RESULTS: Patients showed a substantially lower concentration of Klotho than healthy controls (P < .01). Klotho concentrations were highly correlated with National Institutes of Health Stroke Scale scores, Glasgow coma scale scores, intracerebral hemorrhage scores and hematoma volumes (r = -0.426, 0.382, -0.334 and - 0.432). Patients with the highest plasma Klotho concentration were less prone to have poor prognosis at 90 days compared with the lowest quartile (odds ratio, 0.092; 95% confidence interval, 0.015-0.562). Its optimal cutoff value for distinguishing patients at risk of poor prognosis was 345 pg/ml, which yielded a sensitivity value of 0.86 and a specificity value of 0.62. CONCLUSIONS: Decreased plasma Klotho concentrations were associated with increasing severity and poor prognosis significantly, indicating the prognostic role of plasma Klotho in intracerebral hemorrhage.

5.
Microb Pathog ; 135: 103641, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31330262

RESUMO

Atractylodes lancea is a traditional Chinese perennial herb, which has been used for treating gastrointestinal diseases in traditional medicine. The aim of this study was to investigate the effect of Atractylodes lancea volatile oils on the planktonic growth and biofilm formation of Helicobacter pylori (H. pylori). Firstly, the minimal inhibitory concentration (MIC) of the volatile oils against H. pylori were determined using broth dilution method. SPSS17.0 was used to account 50% inhibiting concentration (IC50). Moreover, the anti-biofilm activity of the volatile oils was determined by crystal violet measurement and fluorescence microscope. Finally, gastric epithelial cells (GES-1 cells) were co-incubated with H. pylori with or without volatile oils treated. Real-time PCR and western blot were performed to detect the translocation of virulence factor Cag A. We found that Atractylodes lancea volatile oils inhibited the growth of H. pylori in a concentration dependent manner. The MIC and IC50 of volatile oils against H. pylori were 7.5 mg/mL and 2.181 mg/mL respectively. Fluorescence microscopy and crystal violet measurement indicated that volatile oils at sub-MIC concentration could reduce biofilm formation of H. pylori. In addition, volatile oils decreased the translocation of Cag A and reduced inflammatory cytokine IL-8 in GES-1 cells. Our results suggested that Atractylodes lancea volatile oils could be a potential compound of a novel class of H. pylori inhibitors with anti-H. pylori effects.

6.
Plant Signal Behav ; 14(8): 1629266, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31184293

RESUMO

It is widely recognized that different parts of a plant can communicate with each other via light-dependent long-distance signaling under heterogeneous light conditions. However, the mechanism of such systemic signaling has not been revealed yet. Our studies on different species suggest the involvement of carbohydrates in light-dependent systemic regulation between different parts of a plant under both short- and long-term light heterogeneity. Leaves exposed to better light condition perform enhanced photosynthetic capacity, and act to compensate for the decline in photosynthesis of other leaves under bad light condition within the same plant. This kind of compensatory photosynthesis has a close relationship to the distribution of carbohydrates, and can be regarded as an integrative strategy to make efficient use of sunlight at the whole-plant level.

7.
Water Res ; 161: 308-318, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31203036

RESUMO

In this study, oxytetracycline (OTC) as a target pollutant in swine wastewater was removed by aerobic granular sludge (AGS). The removal rate of 300 µg/L OTC in aerobic granular sludge sequencing batch reactor (AGSBR) increased to 88.00% in 33 days and maintained stable. The chemical oxygen demand (COD), ammonium nitrogen (NH4+-N) and total phosphorus (TP) in wastewater were also efficiently removed. The removal of OTC mainly depended on the adsorption and biodegradation of AGS, and the biodegradation was increased obviously after AGS adaptation to OTC. The degradation products of OTC were analyzed by mass spectrometry. The analysis of metagenome sequencing revealed that the enzymes, such as glycosyl transferases (GTs), polysaccharide lyases (PLs) and auxiliary activities (AAs), may play an important role in the removal of OTC. The Lefse analysis showed that the Flavobacteriia, Flavobacteriales, Cryomorphaceae and Fluviicola were four kinds of microbes with significant difference in OTC feed reactor, which are considered to be drug-resistant bacteria in AGSBR. Furthermore, the dynamics of microbial community changed significantly at three levels, including the enrichment of drug-resistant microorganisms and the microorganisms that gradually reduced or even disappeared under the pressure of OTC.

8.
PLoS One ; 14(4): e0215911, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31039173

RESUMO

For most complex traits, the majority of SNPs identified through genome-wide association studies (GWAS) reside within noncoding regions that have no known function. However, these regions are enriched for the regulatory enhancers specific to the cells relevant to the specific trait. Indeed, many of the GWAS loci that have been functionally characterized lie within enhancers that regulate expression levels of key genes. In order to identify polymorphisms with potential allele-specific regulatory effects, we developed a bioinformatics pipeline that harnesses epigenetic signatures as well as transcription factor (TF) binding motifs to identify putative enhancers containing a SNP with potential allele-specific TF binding in linkage disequilibrium (LD) with a GWAS-identified SNP. We applied the approach to GWAS findings for blood lipids, revealing 7 putative enhancers harboring associated SNPs, 3 of which lie within the introns of LCAT and ABCA1, genes that play crucial roles in cholesterol biogenesis and lipoprotein metabolism. All 3 enhancers demonstrated allele-specific in vitro regulatory activity in liver-derived cell lines. We demonstrated that these putative enhancers are in close physical proximity to the promoters of their respective genes, in situ, likely through chromatin looping. In addition, the associated alleles altered the likelihood of transcription activator STAT3 binding. Our results demonstrate that through our approach, the LD blocks that contain GWAS signals, often hundreds of kilobases in size with multiple SNPs serving as statistical proxies to the true functional site, can provide an experimentally testable hypothesis for the underlying regulatory mechanism linking genetic variants to complex traits.

9.
Anal Sci ; 35(8): 903-909, 2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31061240

RESUMO

Ferrocenylmethanol (Fc-OH) is included in ß-cyclodextrin (ß-CD) to form the ß-CD-Fc-OH complex by host-guest supramolecular interaction. ß-CD dissociates from the ß-CD-Fc-OH complex due to the conversion of Fc-OH to Fc+-OH under a stimulus of oxidant. In our study, Fc-OH is oxidized after a series of enzymatic reactions of creatinine, which blocks the other means for oxidation of Fc-OH. And the background noise is reduced for testing for serum creatinine (sCr). The chronoamperometry signal for creatinine (with a constant potential -0.3 V vs. Ag/AgCl) increases linearly in the 1 - 1000 µM range, with a limit of detection as low as 0.5 µM. The amperometric potential of -0.3 V greatly prevents the interference of various redox substances in serum. The biosensor was used to test 120 clinical specimens and the results showed a linear correlation with the biochemical analyzer (R2 = 0.9885). The biosensor could be applied to clinical trials and offers good prospects for clinical sCr detection.

10.
Gene ; 709: 75-83, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31129249

RESUMO

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative pathogen for porcine reproductive and respiratory syndrome (PRRS), which lead to huge loss to porcine industry. RACK1 (receptor of activated protein C kinase 1) was first identified as a receptor for protein kinase C. Mounting evidence demonstrated that RACK1 played diverse roles in NF-κB activation and virus infections. We previously reported that siRNA knockdown of RACK1 inhibited PRRSV replication in Marc-145 cells, abrogated NF-κB activation induced by PRRSV infection and reduced the viral titer. Here we established a Marc-145 cell line which could stably overexpress RACK1 to consolidate our findings. Based on the data from RT-qPCR, western blot, immunofluorescence staining, cytopathic effects and viral titer analysis, we concluded that overexpression of RACK1 could enhance the replication of PRRSV in Marc-145 cells and promote the NF-κB activation via upregulating TRAF2 expression and its phosphorylation. Marc-145 cells overexpressing RACK1exhibited severe cytopathic effects post infection with PRRSV and elevated the viral titer. Taken together, RACK1 plays an essential role for PRRSV replication in Marc-145 cells and NF-κB activation. The results presented here shed more light on the understanding of the molecular mechanisms underlying PRRSV infection and its subsequent NF-κB activation. Therefore, we anticipate RACK1 as a promising target for PRRS control.


Assuntos
NF-kappa B/metabolismo , Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia , Receptores de Quinase C Ativada/genética , Fator 2 Associado a Receptor de TNF/genética , Fator 2 Associado a Receptor de TNF/metabolismo , Replicação Viral/genética , Animais , Linhagem Celular , Regulação da Expressão Gênica , Macaca mulatta , Fosforilação , Proteínas Quinases/metabolismo , Receptores de Quinase C Ativada/metabolismo , Transdução de Sinais/genética , Ativação Transcricional , Regulação para Cima
11.
Eur Radiol ; 29(7): 3325-3337, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30972543

RESUMO

OBJECTIVES: To develop and validate a radiomics nomogram to preoperative prediction of isocitrate dehydrogenase (IDH) genotype for astrocytomas, which might contribute to the pretreatment decision-making and prognosis evaluating. METHODS: One hundred five astrocytomas (Grades II-IV) with contrast-enhanced T1-weighted imaging (CE-T1WI), T2 fluid-attenuated inversion recovery (T2FLAIR), and apparent diffusion coefficient (ADC) map were enrolled in this study (training cohort: n = 74; validation cohort: n = 31). IDH1/2 genotypes were determined using Sanger sequencing. A total of 3882 radiomics features were extracted. Support vector machine algorithm was used to build the radiomics signature on the training cohort. Incorporating radiomics signature and clinico-radiological risk factors, the radiomics nomogram was developed. Receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to assess these models. Kaplan-Meier survival analysis and log rank test were performed to assess the prognostic value of the radiomics nomogram. RESULTS: The radiomics signature was built by six selected radiomics features and yielded AUC values of 0.901 and 0.888 in the training and validation cohorts. The radiomics nomogram based on the radiomics signature and age performed better than the clinico-radiological model (training cohort, AUC = 0.913 and 0.817; validation cohort, AUC = 0.900 and 0.804). Additionally, the survival analysis showed that prognostic values of the radiomics nomogram and IDH genotype were similar (log rank test, p < 0.001; C-index = 0.762 and 0.687; z-score test, p = 0.062). CONCLUSIONS: The radiomics nomogram might be a useful supporting tool for the preoperative prediction of IDH genotype for astrocytoma, which could aid pretreatment decision-making. KEY POINTS: • The radiomics signature based on multiparametric and multiregional MRI images could predict IDH genotype of Grades II-IV astrocytomas. • The radiomics nomogram performed better than the clinico-radiological model, and it might be an easy-to-use supporting tool for IDH genotype prediction. • The prognostic value of the radiomics nomogram was similar with that of the IDH genotype, which might contribute to prognosis evaluating.


Assuntos
Astrocitoma/genética , Isocitrato Desidrogenase/genética , Nomogramas , Adulto , Algoritmos , Área Sob a Curva , Astrocitoma/diagnóstico por imagem , Astrocitoma/patologia , Astrocitoma/cirurgia , Sistemas de Apoio a Decisões Clínicas , Imagem de Difusão por Ressonância Magnética/métodos , Feminino , Genótipo , Humanos , Interpretação de Imagem Assistida por Computador/métodos , Estimativa de Kaplan-Meier , Imagem por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Cuidados Pré-Operatórios/métodos , Prognóstico , Curva ROC , Reprodutibilidade dos Testes , Estudos Retrospectivos , Fatores de Risco , Máquina de Vetores de Suporte , Adulto Jovem
12.
Mol Genet Genomic Med ; 7(6): e672, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30972954

RESUMO

BACKGROUND: The serum tumor markers has been widely used in ovarian cancer diagnosis. BRCA1/2 germline mutations are the most common predisposing factors for ovarian cancer development. This study aimed to comprehensively investigate serum tumor markers and BRCA1/2 germline mutations and analyze their associations with ovarian cancer. METHODS: Levels of 11 serum tumor markers were examined in ovarian cancer patients and controls with benign gynecologic diseases. By integrating multiplex PCR and next-generation sequencing technologies, BRCA1/2 germline mutations were analyzed and confirmed by Sanger sequencing. The discriminative models with serum tumor markers and BRCA1/2 mutation status were constructed for ovarian cancer detection and patient stratification. RESULTS: Among 11 markers, six of them were significantly elevated and only beta-human chorionic gonadotropin (ß-HCG) was significantly reduced in ovarian cancer patients. A total of 54 (23.3%) ovarian cancer patients were found to harbor BRCA1/2 deleterious mutations, and BRCA1/2 mutations were significantly associated with Hereditary Breast and Ovarian Cancer-related tumors and family history of cancer. Carbohydrate antigen 125 showed a good performance in ovarian cancer detection as a single marker (AUC = 0.799), while a panel of eight markers showed a good performance in BRCA1 mutation detection with an AUC value of 0.974. In addition, a panel of five serum tumor markers combined with BRCA1/2 mutation status showed a good performance in lymph node metastasis prediction (AUC = 0.843). CONCLUSIONS: We found the association between BRCA1/2 germline mutation status and serum tumor marker levels, and identified discriminative models that combined serum tumor markers with BRCA1/2 mutation status for ovarian cancer detection and patient stratification.

13.
BMC Ophthalmol ; 19(1): 67, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30845992

RESUMO

BACKGROUND: To evaluate the ocular surface characteristics and the infestation of Demodex in Chinese paediatric and adult blepharokeratoconjunctivitis (BKC). METHODS: Fifty consecutive patients with BKC and 50 age- and sex-matched healthy subjects were enrolled. Lid margin characteristics and corneal disorders were evaluated under slit-lamp illumination. Four eyelashes were collected from each eye to examine Demodex infestation by light microscopy. RESULTS: Corneal neovascularization (P = 0.001) and scarring (P = 0.040) were significantly worse in children than in adults with BKC, whereas meibum quality was worse in adults (P = 0.008). Diagnosis delay was longer in children with BKC than in adults (2.2 vs 1.2 years, P = 0.022). Demodex infestation was more frequent in subjects with BKC than in healthy subjects (56% vs 26%, P = 0.002). The lid margin inflammation and meibomian gland dysfunction were worse in Demodex-positive subjects than in Demodex-negative subjects with BKC. CONCLUSIONS: Children with BKC had severer corneal disorders compared with adult BKC patients, which may be caused by a long-delayed diagnosis. Ocular demodicosis was more common in subjects with BKC. Ocular Demodex infestation was associated with worse lid margin inflammation and meibomian gland dysfunction.


Assuntos
Blefarite/parasitologia , Conjuntivite/parasitologia , Doenças da Córnea/patologia , Pestanas/parasitologia , Doenças Palpebrais/parasitologia , Infestações por Ácaros/complicações , Adolescente , Adulto , Estudos de Casos e Controles , Criança , Pré-Escolar , Doenças da Córnea/etiologia , Neovascularização da Córnea/patologia , Diagnóstico Tardio , Doenças Palpebrais/complicações , Pálpebras/patologia , Feminino , Humanos , Masculino , Glândulas Tarsais/patologia , Pessoa de Meia-Idade , Adulto Jovem
14.
Arch Virol ; 164(4): 1059-1067, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30783771

RESUMO

The virome in genital tract secretion samples collected from 80 dairy cattle in Shanghai, China, was characterized. Viruses detected included members of the families Papillomaviridae, Polyomaviridae, Hepeviridae, Parvoviridae, Astroviridae, Picornaviridae, and Picobirnaviridae. A member of a new species within the genus Dyoxipapillomavirus and six circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viral genomes were fully sequenced and phylogenetically analyzed. The prevalence of bovine polyomaviruses 1 and 2 was measured by PCR to be 10% (8/80) and 6.25% (5/80), respectively. PCR screening also indicated that the novel papillomavirus ujs-21015 and bovine herpesvirus 6 were present in three and two out of the 80 samples, respectively.


Assuntos
Vagina/virologia , Vírus/genética , Vírus/isolamento & purificação , Animais , Bovinos , China , DNA Viral/genética , Feminino , Genoma Viral , Sequenciamento de Nucleotídeos em Larga Escala , Metagenômica , Filogenia , Vírus/classificação
15.
J Environ Sci (China) ; 78: 127-136, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30665631

RESUMO

Two types of inoculated sludges, granular sludge that had been stored at -20°C and activated sludge, were investigated for the domestication of aerobic granular sludges (AGSs) in sequencing batch reactors (SBRs). The results showed that using the stored granular sludge as inoculation sludge could effectively shorten the domestication time of AGS and yielded mature granular sludge after 22 days of operation. The AGS domesticated by stored granular sludge had better biomass and sedimentation properties; its MLSS and SVI reached 8.55 g/L and 35.27 mL/g, respectively. The removal efficiencies for chemical oxygen demand (COD), ammonium nitrogen (NH4+-N) and total phosphorus (TP) reached 90.76%, 97.39% and 96.40%, respectively. By contrast, 54 days were needed to obtain mature granules using activated sludge. The microbial community structure was probed by using scanning electron microscopy (SEM) and high-throughput sequencing. The results showed that the diversity of the microbial community in mature granules was reduced when stored granular sludge rather than activated sludge was employed as inoculation sludge, and the dominant microbes were changed. The dominant species in mature granules domesticated using stored granular sludge were Zoogloea, Acidovorax and Tolumonas at the genus classification level, while the dominant species were Zoogloea and TM7-genera in granules developed from activated sludge.


Assuntos
Reatores Biológicos , Esgotos/química , Eliminação de Resíduos Líquidos/métodos , Aerobiose , Compostos de Amônio , Análise da Demanda Biológica de Oxigênio , Biomassa , Nitrogênio , Fósforo , Esgotos/microbiologia , Poluentes Químicos da Água
16.
Sci Total Environ ; 657: 987-999, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30677964

RESUMO

Sustainable agricultural development is urgently required to satisfy future food demands while decreasing environmental costs. Intercropping can increase per-unit farmland productivity through a resource-efficient utilization. However, the fate of N in intercropping systems remains unclear. To study the yield advantages and the fate of N in additive maize-soybean relay intercropping (IMS) systems, we quantified crop yield, soil N transformation abilities, soil bacterial abundances, and the fate of 15N. This study was conducted using three planting patterns, namely, monoculture maize (Zea mays L.) (MM), monoculture soybean (Glycine max L. Merr.) (MS), and IMS, and two N application rates, specifically, no N and applied N (N1, 45 and 135 kg N ha-1 for MS and MM, correspondingly; and N for the IMS, which was the sum of the monocultures). Results showed that a higher per-unit farmland productivity and a lower land use intensity are attained in the intercropping system than in the corresponding monocultures. In addition, land equivalent ratio (LER) ranges from 1.85 to 2.20. Moreover, the fate of 15N showed that the N uptake and residual are the highest, whereas N loss in the IMS is the lowest among all planting patterns. Intercropping had an increased N use efficiency by increasing N utilization efficiency, rather than N uptake efficiency. The abundance of ammonia oxidizer and denitrifier indicated that IMS improves the structure of soil microorganisms. Furthermore, the transformation abilities of soil N denoted that intercropping strengthens ammonifying and nitrifying capacities to increase soil N residual while decreasing ammonia volatilization and N2O emission. Finally, the greenhouse warming potential and gas intensity of N2O were significantly lower in the IMS than in the corresponding monocultures. In summary, the IMS system provides an environmentally friendly approach to increasing farmland productivity.


Assuntos
Agricultura/métodos , Nitrogênio/análise , Solo/química , Soja/crescimento & desenvolvimento , Zea mays/crescimento & desenvolvimento , China , Produtos Agrícolas/crescimento & desenvolvimento , Desnitrificação/genética , Fertilizantes , Expressão Gênica , Nitrogênio/metabolismo
17.
Bioresour Technol ; 271: 258-265, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30278350

RESUMO

Carbon to nitrogen (C/N) ratio is one of the most important factor affecting aerobic granular sludge (AGS) growth and pollutant removal in aerobic granular sludge sequencing batch reactor (AGSBR). For stability of sludge granulation process, AGSs were domesticated in five sequence batch reactors (SBRs) with different C/N ratios (6, 7, 8, 9, and 10), which the ammonia nitrogen concentration of influent was 165 mg/L. The effects of C/N ratio on morphology and property of AGS were studied. The results showed that stable AGS was yielded with good settleability, high pollutant removal efficiency and rich microbial diversity when C/N ratio was 8. AGS yielded had stable structure due to higher protein in extracellular polymeric substances (EPS). High throughput 16S rDNA gene analysis revealed the microbial community diversity increased in AGS under the C/N ratio. The dominant microbes changed at the phylum, class and family three levels with the increasing operation time.


Assuntos
Carbono/análise , Nitrogênio/análise , Esgotos/microbiologia , Aerobiose , Amônia/análise , DNA Ribossômico/genética , Dinâmica Populacional , Esgotos/química , Eliminação de Resíduos Líquidos/métodos
18.
Int J Infect Dis ; 80: 16-27, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30529371

RESUMO

BACKGROUND AND AIM: Chronic hepatitis C is a major global health challenge. China has the world's largest burden of hepatitis C and related liver disease, with several groups having an increased risk of acquiring hepatitis C virus (HCV) infection. We undertook a systematic review in Chinese and English literature to determine the seroprevalence of anti-HCV among men who have sex with men (MSM), sex workers, people who use drugs (PWUD) and migrant workers in China. METHODS: We searched three Chinese databases (CNKI, Wanfang and Chongqing VIP Information) and four English databases (Pubmed, EMBASE, Global Health and CINAHL) for studies published between 2010 and 2015. Included were 89 studies for MSM, 112 for sex workers, 11 for migrant workers, 94 for people who inject drugs (PWID) and 67 for non-injectors. Random-effect models pooled estimates of anti-HCV seroprevalence in each population and a meta-regression model examined the relationship between anti-HCV seroprevalence and injecting drug use. RESULTS: The overall pooled seroprevalence of anti-HCV among MSM was 0.67% (CI 95 0.51-0.86); for sex workers 0.65% (CI 95 0.53-0.77); for migrant workers 0.48 (CI 95 0.20-0.85); for IDUs 72.41% (CI 95 68.71-75.97); among non-injectors 25.07% (CI 95 21.51%-28.80%). Our meta-regression model predicted that the seroprevalence of anti-HCV among PWID increased by 8.6% for each 10% increase seroprevalence of reporting ever having injected drugs. CONCLUSIONS: Overall, seroprevalence of HCV infection is high among PWUD, especially those who inject. Lower HCV seroprevalence (<1%) was found among MSM, sex workers and migrant workers. Our estimates for IDU seroprevalence are higher than that from the national surveillance system, though estimates for other at-risk populations are similar.


Assuntos
Hepatite C/epidemiologia , China/epidemiologia , Bases de Dados Factuais , Usuários de Drogas , Hepacivirus/isolamento & purificação , Homossexualidade Masculina , Humanos , Masculino , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Profissionais do Sexo , Migrantes
19.
Head Neck ; 41(1): 46-55, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30561022

RESUMO

BACKGROUND: Photon (X-ray) radiotherapy (XRT) kills cells via DNA damage, however, how proton radiotherapy (PRT) causes cell death in head and neck squamous cell carcinoma (HNSCC) is unclear. We investigated mechanisms of HNSCC cell death after XRT versus PRT. METHODS: We assessed type of death in 2 human papillomavirus (HPV)-positive and two HPV-negative cell lines: necrosis and apoptosis (Annexin-V fluorescein isothiocyanate [FITC]); senescence (ß-galactosidase); and mitotic catastrophe (γ-tubulin and diamidino-phenylindole [DAPI]). RESULTS: The XRT-induced or PRT-induced cellular senescence and mitotic catastrophe in all cell lines studied suggested that PRT caused cell death to a greater extent than XRT. After PRT, mitotic catastrophe peaked in HPV-negative and HPV-positive cells at 48 and 72 hours, respectively. No obvious differences were noted in the extent of cell necrosis or apoptosis after XRT versus PRT. CONCLUSION: Under the conditions and in the cell lines reported here, mitotic catastrophe and senescence were the major types of cell death induced by XRT and PRT, and PRT may be more effective.

20.
Phys Med Biol ; 2018 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-30523805

RESUMO

In charged particle therapy, the objective is to exploit both the physical and radiobiological advantages of charged particles to improve the therapeutic index. Use of the beam scanning technique provides the flexibility to implement biological dose optimized intensity-modulated ion therapy (IMIT). An easy-to-implement algorithm was developed in the current study to rapidly generate a uniform biological dose distribution, namely the product of physical dose and the relative biological effectiveness (RBE), within the target volume using scanned ion beams for charged particle radiobiological studies. Protons, helium ions and carbon ions were selected to demonstrate the feasibility and flexibility of our method. The general-purpose Monte Carlo simulation toolkit Geant4 was used for particle tracking and generation of physical and radiobiological data needed for later dose optimizations. The dose optimization algorithm was developed using the Python (version 3) programming language. A constant RBE-weighted dose (RWD) spread-out Bragg peak (SOBP) in a water phantom was selected as the desired target dose distribution to demonstrate the applicability of the optimization algorithm. The mechanistic repair-misrepair-fixation (RMF) model was incorporated into the Monte Carlo particle tracking to generate radiobiological parameters and was used to predict the RBE of cell survival in the iterative process of the biological dose optimization for the three selected ions. The post-optimization generated beam delivery strategy can be used in radiation biology experiments to obtain radiobiological data to further validate and improve the accuracy of the RBE model. This biological dose optimization algorithm developed for radiobiology studies could potentially be extended to implement biologically optimized IMIT plans for patients.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA