Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.114
Filtrar
1.
Environ Sci Technol ; 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32401498

RESUMO

A massive ecological restoration program has been implemented in northern China with the aim of protecting the Beijing-Tianjin-Hebei metropolitan area of eastern China from dust events. However, some current studies have cast doubt on the efficacy of such ecological restoration projects, partly due to the constraint of available water in northern China, leading to poor survival rates of planted trees in semiarid regions (15%). In this study, using a logical framework combining statistical analysis, partial least squares path model analysis and a regional climate model (RegCM) simulation with multi-source dust indicators, we found that there was a reduction of dust in northern China that was synchronous with the increase in vegetation growth after ecological restoration. In contrast to previous reports of a decrease in wind speed due to ecological restoration, this study found that the increase in vegetation had an insignificant impact on local wind speed (p=0.30). Instead, ecological restoration mainly reduced the sand emission in steppe area by improving the soil conditions of the underlying surface, and hence contributed 15% of the reduction of dust events in the Beijing-Tianjin-Hebei metropolitan area through dust transmission (p=0.002). The effect of ecological restoration in the northern steppe on dust reduction over the northeastern metropolitan area of China should not be overstated.

2.
Chemosphere ; 256: 127064, 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32454352

RESUMO

Passive air samplers comprising sorbent-Tenax-TA thermal desorption tubes were applied for monitoring cyclic volatile methyl siloxanes (cVMSs) in the air above the aeration tanks of eight wastewater treatment plants (WWTPs, including five "open" and three "close" plants) in Dalian, China. The aim was to investigate the inputs of cVMSs from WWTPs to the air throughout a year (June 2016-July 2017). The ∑cVMS concentrations were averaged at 3.14 ± 6.84 µg·m-³ with D5 as the dominant compound (contributing to 63.8% of the ∑cVMS concentration). The annual average concentrations of D4, D5, and D6 in the air of the "close" WWTPs were 5.33 ± 4.63, 13.4 ± 14.4, and 1.05 ± 1.47 µg·m-³, and 1.31 ± 1.29, 1.57 ± 2.01, and 0.301 ± 0.340 µg·m-³ in the air from the "open" WWTPs, respectively. For both the close and open aeration tanks, the concentration of ∑cVMSs was the highest in summer and the lowest in winter, showing a significant correlation with ambient temperature (linear regression; p < 0.01). A simplified Gaussian dispersion model and a single chamber model were introduced to estimate annual emissions (kg·yr-1) of cVMSs from "open" and "close" WWTPs, ranging from 86.9 to 165 kg yr-1 and from 203 to 278 kg yr-1, respectively. Examining the relationship between the per capita emissions of cVMSs and average property prices, our results indicated that a greater amount of personal care products were used/discharged by people with relatively higher socioeconomic status.

3.
Cell Mol Biol (Noisy-le-grand) ; 66(1): 65-69, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32359386

RESUMO

Osteoarthritis (OA) is a clinical disease which seriously affects the quality of life of sufferers. Although the pathogenesis of OA has not been fully unraveled, it is may be due to increased levels of pro-inflammatory cytokines, activation of inflammation-related signaling pathways, and degradation of extracellular matrix. Osteoarthritis is characterized by chronic joint pain, swelling, stiffness, limited movement or joint deformity, all of which seriously affect the quality of life and health of the affected individuals. Myroside (Myr) is a polyphenolic hydroxyflavone glycoside extracted from the fruits, bark and leaves of myroside and other natural plants. It has many pharmacological properties, especially anti-inflammatory effects. In the present study, primary chondrocytes of IL-1ß rats were used to simulate pathological environment of chondrocytes in OA, and to explore the effect of Myr on chondrocytes. It was found that Myr improved the viability and proliferation of chondrocytes, and also inhibited apoptosis in these cells. Moreover, Myr reduced the expressions of inflammatory factors, and inhibited inflammatory reactions in chondrocytes. These findings provide good experimental basis for the clinical application of Myr in the prevention and treatment of progressive degeneration of cartilage in OA.

4.
Gastric Cancer ; 2020 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-32388635

RESUMO

BACKGROUND: Increasing evidence indicates that angiogenesis plays an important role in tumor progression. The function of cathepsin L (CTSL), an endosomal proteolytic enzyme, in promoting tumor metastasis is well recognized. The mechanisms by which CTSL has promoted the angiogenesis of gastric cancer (GC), however, remains unclear. METHODS: The nuclear expression levels of CTSL were assessed in GC samples. The effects of CTSL on GC angiogenesis were determined by endothelial tube formation analysis, HUVEC migration assay, and chick embryo chorioallantoic membrane (CAM) assay. The involvement of the CDP/Cux/VEGF-D pathway was analyzed by angiogenesis antibody array, Western blot, co-immunoprecipitation (Co-IP) and dual-luciferase reporter assay. RESULTS: In this study, we found that the nuclear CTSL expression level in GC was significantly higher than that in adjacent nontumor gastric tissues and was a potential important clinical prognostic factor. Loss- and gain-of-function assays indicated that CTSL promotes the tubular formation and migration of HUVEC cells in vitro. The CAM assay also showed that CTSL promotes angiogenesis of GC in vivo. Mechanistic analysis demonstrated that CTSL can proteolytically process CDP/Cux and produce the physiologically relevant p110 isoform, which stably binds to VEGF-D and promotes the transcription of VEGF-D, thus contributing to the angiogenesis of GC. CONCLUSION: The findings of the present study suggested that CTSL plays a constructive role in the regulation of angiogenesis in human GC and could be a potential therapeutic target for GC.

5.
Clin Lung Cancer ; 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32371161

RESUMO

BACKGROUND: Patients eligible and referred for lung cancer screening (LCS) may not complete the visit. We aimed to identify differences in demographic, clinical, and socioeconomic characteristics between LCS participants and LCS-eligible nonparticipants and determine potential reasons for nonparticipation. MATERIALS AND METHODS: LCS-eligible patients referred between April 2015 and August 2016 were divided into participants and nonparticipants. Retrospective data were collected. A telephone survey was conducted in a subset of nonparticipants to identify reasons for not participating and assess their understanding of the benefits and harms of LCS. RESULTS: We identified 542 participants and 276 LCS-eligible nonparticipants. Female sex, lower pack-years, active smoking, the absence of a history of chronic obstructive pulmonary disease, coronary artery disease, or liver disease, and chronic kidney disease or a history of malignancy were associated with not participating. One hundred nonparticipants completed the telephone survey-29% were unaware of the appointment; 19% had concerns about the distance from the screening site and 14% with insurance coverage; 10% had fear of the imaging result; and 10% felt the computed tomography scan was unnecessary. Eighteen percent knew the benefit of LCS, and 69% were not aware of any screening harms. CONCLUSIONS: LCS-eligible nonparticipants are more likely to be female, active smokers, have fewer total pack-years of smoking, chronic kidney disease, a history of prior malignancy, and not have chronic obstructive pulmonary disease, coronary artery disease, or liver disease. Targeted education about the benefits and harms of LCS, verification of insurance coverage, and providing convenient screening locations may improve participation.

6.
Bioorg Chem ; 99: 103812, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32302796

RESUMO

Alopecines A-E (1-5), five unusual matrine-type alkaloids featuring with an additional dichlorocyclopropane (1-3) or a di/tri-chloromethyl (4/5) attached on the D ring, were isolated from the seeds of Sophora alopecuroides. Their structures and absolute configurations were elucidated by extensive spectroscopic techniques, and X-ray diffraction analyses or time-dependent density functional theory-based electronic circular dichroism (TDDFT-ECD) calculations. Alkaloid 4 exhibited potent inhibitory effects on the proliferation of ConA-induced T lymphocytes or LPS-induced B cells with IC50 value of 3.98 or 3.74 µM, respectively.

7.
Medicine (Baltimore) ; 99(17): e19659, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32332609

RESUMO

BACKGROUND: The number of citations a scientific paper has received indicates its impact within any medical field. We performed a bibliometric analysis to highlight the key topics of the most frequently cited 100 articles on perianal fistula to determine the advances in this field. METHODS: The Scopus database was searched from 1960 to 2018 using the search terms "perianal fistula" or "anal fistula" or "fistula in ano" or "anal fistulae" or "anorectal fistulae" including full articles. The topic, year of publication, publishing journal, country of origin, institution, and department of the first author were analyzed. RESULTS: The median number of citations for the top 100 of 3431 eligible papers, ranked in order of the number of citations, was 100 (range: 65-811), and the number of citations per year was 7.5 (range: 3.8-40.1). The most-cited paper (by Parks et al in 1976; 811citations) focused on the classification of perianal fistula. The institution with the highest number of publications was St Mark's Hospital, London, UK. The most-studied topic was surgical management (n = 47). The country and the decade with the greatest number of publications in this field were the USA (n = 34) and the 2000s (n = 50), respectively. CONCLUSION: The 100 most frequently cited manuscripts showed that surgical management had the greatest impact on the study of perianal fistula. This citation analysis provides a reference of what could be considered the most classic papers on perianal fistula, and may serve as a reference for researchers and clinicians as to what constitutes a citable paper in this field.


Assuntos
Bibliometria , Fístula Retal/epidemiologia , Fístula Retal/cirurgia , Humanos , Publicações Periódicas como Assunto
8.
BMC Med Inform Decis Mak ; 20(1): 64, 2020 04 06.
Artigo em Inglês | MEDLINE | ID: mdl-32252745

RESUMO

BACKGROUND: In this study, we focus on building a fine-grained entity annotation corpus with the corresponding annotation guideline of traditional Chinese medicine (TCM) clinical records. Our aim is to provide a basis for the fine-grained corpus construction of TCM clinical records in future. METHODS: We developed a four-step approach that is suitable for the construction of TCM medical records in our corpus. First, we determined the entity types included in this study through sample annotation. Then, we drafted a fine-grained annotation guideline by summarizing the characteristics of the dataset and referring to some existing guidelines. We iteratively updated the guidelines until the inter-annotator agreement (IAA) exceeded a Cohen's kappa value of 0.9. Comprehensive annotations were performed while keeping the IAA value above 0.9. RESULTS: We annotated the 10,197 clinical records in five rounds. Four entity categories involving 13 entity types were employed. The final fine-grained annotated entity corpus consists of 1104 entities and 67,799 tokens. The final IAAs are 0.936 on average (for three annotators), indicating that the fine-grained entity recognition corpus is of high quality. CONCLUSIONS: These results will provide a foundation for future research on corpus construction and named entity recognition tasks in the TCM clinical domain.

10.
Artigo em Inglês | MEDLINE | ID: mdl-32306261

RESUMO

Park playgrounds recently are suffering serious heavy metals contamination in China. It is urgent to assess the ecological risk and identify the sources for heavy metals. A total of 111 topsoil samples were collected from four park playgrounds in Xi'an, and the X-ray fluorescence (XRF) instrument was used to measure the concentrations of heavy metals including chromium(Cr), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), lead (Pb), manganese (Mn), and cobalt (Co), respectively. Ecological risk ([Formula: see text]) and potential ecological risk index (RI) were introduced to determine the pollution level and ecological risk, and the absolute principal component score-multiple linear regression (APCS-MLR) model was implemented to identify the sources for heavy metals. The main results were as follows. (1) Except As, the mean concentrations of measured heavy metals of four park playgrounds surpassed the soil background values of Shaanxi Province. (2) In each park playground, the [Formula: see text] was below a "low" risk level ([Formula: see text]=10) for Cr, Ni, Zn, As, and Mn; Cu was between a "moderate" and "considerable" risk level; Pb was between a "low" and "moderate" risk level; and [Formula: see text] was between a "considerable" and "high" risk level for Co. Besides, the RI index was on a "high" risk level (120 < RI < 240) with an obvious spatial distinction. (3) The anthropogenic factors were the main sources for heavy metals, and mixed sources and natural sources were considered as the minor sources for metals. (4) The sources contributions for Co had obvious spatial heterogeneity in each park situated in four different urban planning districts.

12.
Cancer Biother Radiopharm ; 35(4): 307-312, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32191497

RESUMO

Background: Long noncoding RNA (lncRNA) LUADT1 is a known oncogenic lncRNA in lung cancer. This study aimed to explore the roles of LUADT1 in melanoma. Materials and Methods: Sixty pairs of melanoma and nontumor tissues were obtained from 60 melanoma patients (37 men and 23 women, 38-68 years, 52.1 ± 4.9 years) at the First Affiliated Hospital of Zhejiang University School of Medicine. Gene expression was analyzed by quantitative polymerase chain reaction and western blot. Cell transfections were performed to analyze gene expression. Results: We found that LUADT1 was upregulated in melanoma and high levels of LUADT1 predicted poor survival. RNA interaction prediction showed that LUADT1 can form base pairing with miR-28-5p. In melanoma cells, LUADT1 overexpression mediated the upregulated Ras-related protein Rap-1b (RAP1B). Cell proliferation assay showed that LUADT1 and RAP1B overexpression mediated the increased proliferation rate of melanoma cells. In addition, miR-28-5p overexpression played opposite roles attenuating the effects of LUADT1 overexpression on both RAP1B expression and cancer cell proliferation. Conclusions: LUADT1 in melanoma and may sponge miR-28-5p to upregulate RAP1B, thereby promoting cancer cell proliferation.

13.
Cell Biol Int ; 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32125046

RESUMO

Long non-coding RNA (lncRNA) SNHG16 has been reported to be significant regulators in multiple cancers. However, never has the relationship between it and cardiac hypertrophy been studied until now. In this study, angiotensin II (Ang II)-treated cardiomyocytes isolated from neonatal mice were used as a model of cardiac hypertrophy in vitro. Real-time quantitative polymerase chain reaction was performed to measure the expression of SNHG16, miR-182-5p, and insulin-like growth factor 1 (IGF1). The relationship between SNHG16 and its downstream genes were corroborated by RNA pull-down and luciferase reporter experiments. Western blot was conducted to detect the expression of markers of hypertrophy. The results disclosed that SNHG16 expression was in a high level in the cardiac hypertrophic model. Down-regulation of SNHG16 could decline the expression of hypertrophic markers and reduce cell surface area induced by Ang II. Moreover, SNHG16 was discovered to be activated by transcription factor CCCTC-binding factor. In addition, SNHG16 could enlarge cell surface area and increase the expression of hypertrophic markers by inhibiting miR-182-5p expression in Ang II-treated cardiomyocytes. Finally, overexpression of IGF1 could rescue the effects of silenced SNHG16 on cardiac hypertrophy cells. In brief, our study illustrated that silenced SNHG16 repressed Ang II-imposed cardiac hypertrophy via targeting miR-182-5p/IGF1 axis.

15.
Plant Physiol ; 183(1): 331-344, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32165446

RESUMO

A wide variety of intrinsic and extrinsic cues lead to cell death with unclear mechanisms. The infertility of some death mutants often hurdles the classical suppressor screens for death regulators. We have developed a transient RNA interference (RNAi)-based screen using a virus-induced gene silencing approach to understand diverse cell death pathways in Arabidopsis (Arabidopsis thaliana). One death pathway is due to the depletion of a MAP kinase (MAPK) cascade, consisting of MAPK kinase kinase 1 (MEKK1), MKK1/2, and MPK4, which depends on a nucleotide-binding site Leu-rich repeat (NLR) protein SUMM2. Silencing of MEKK1 by virus-induced gene silencing resembles the mekk1 mutant with autoimmunity and defense activation. The RNAi-based screen toward Arabidopsis T-DNA insertion lines identified SUMM2, MEKK2, and Calmodulin-binding receptor-like cytoplasmic kinase 3 (CRCK3) to be vital regulators of RNAi MEKK1-induced cell death, consistent with the reports of their requirement in the mekk1-mkk1/2-mpk4 death pathway. Similar with MEKK2, overexpression of CRCK3 caused dosage- and SUMM2-dependent cell death, and the transcripts of CRCK3 were up-regulated in mekk1, mkk1/2, and mpk4 MEKK2-induced cell death depends on CRCK3. Interestingly, CRCK3-induced cell death also depends on MEKK2, consistent with the biochemical data that MEKK2 complexes with CRCK3. Furthermore, the kinase activity of CRCK3 is essential, whereas the kinase activity of MEKK2 is dispensable, for triggering cell death. Our studies suggest that MEKK2 and CRCK3 exert concerted functions in the control of NLR SUMM2 activation and MEKK2 may play a structural role, rather than function as a kinase, in regulating CRCK3 protein stability.

16.
Sci Rep ; 10(1): 4061, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32132612

RESUMO

Previous studies have found that fine particulate matter (PM2.5) air pollution is associated with decreased lung function. However, most current research focuses on children with asthma, leading to small sample sizes and limited generalization of results. The current study aimed to measure the short-term and lag effects of PM2.5 among school-aged children using repeated measurements of lung function.This prospective panel study included 848 schoolchildren in Zhejiang Province, China. Each year from 2014-2017, two lung function tests were conducted from November 15th to December 31st. Daily air pollution data were derived from the monitoring stations nearest to the schools. A mixed-effects regression model was used to investigate the relationship between PM2.5 and lung function. The effect of PM2.5 on lung function reached its greatest at 1-day moving average PM2.5 exposure. For every 10 µg/m3 increase in the 1-day moving average PM2.5 concentration, Forced Vital Capacity (FVC) of children decreased by 33.74 mL (95% CI: 22.52, 44.96), 1-s Forced Expiratory Volume (FEV1) decreased by 32.56 mL (95% CI: 21.41, 43.70), and Peak Expiratory Flow (PEF) decreased by 67.45 mL/s (95% CI: 45.64, 89.25). Stronger associations were found in children living in homes with smokers. Short-term exposure to PM2.5 was associated with reductions in schoolchildren's lung function. This finding indicates that short-term exposure to PM2.5 is harmful to children's respiratory health, and appropriate protective measures should be taken to reduce the adverse effects of air pollution on children's health.

18.
Artigo em Inglês | MEDLINE | ID: mdl-32197470

RESUMO

To understand the heavy metal pollution in agricultural soils along the East River basin and assess the pollution related health effect to local residents, interviews and archived data were obtained to identify the study sites affected by polluted tailing. Soil samples were collected and tested for heavy metal content and the Comprehensive Pollution Index (CPI). The degree of pollution of agricultural soils in the area was assessed using GIS-based Spatial distribution map of heavy metals and the trend of soil heavy metal risk. Two villages (Matian and Zhudui) near the East River were included in this study for health effect assessment. A total of 193 residents aged 15 or above from each village were tested for the present status of chronic diseases. Convenient sampling method was used to collect blood samples from 78 residents for heavy metal concentration. The contents of Pb, Cd, As, Zn, and Cu in the agricultural soils were all over the standards with a moderate to severe CPI. Among these metals, Cd was the highest followed by Pb, and Cu was the lowest. The contents of Pb, Cd, As, and Zn tend to be higher in soils closer to the river. The prevalence of chronic diseases was over 30%, which is significantly higher than the report from the national central region (23.15%). The average blood lead level (BLL) among children under 14 years is 7.42 µg/dL. Although the adults in Matian village had a significantly higher BLL (χ2 = 8.70, p = 0.03) as compared to Zhudui village, there was no significant difference for the prevalence of chronic diseases between the two villages (χ2 = 3.23, p = 0.09). The mean BLL of children and the proportion of children with BLL ≥ 10 µg/dL in this study are equivalent to the national average. The higher BLL concentration and prevalence of chronic diseases in adults might be due to their long-term exposure to heavy metal contamination environment and higher background level of heavy metals. Findings from this study will form the baseline information for local government to the development of effective approaches to control the heavy metal contamination and reduce the pollution related adverse health effect on local residents.

19.
Sensors (Basel) ; 20(6)2020 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-32188068

RESUMO

Low dose computed tomography (CT) has drawn much attention in the medical imaging field because of its ability to reduce the radiation dose. Recently, statistical iterative reconstruction (SIR) with total variation (TV) penalty has been developed to low dose CT image reconstruction. Nevertheless, the TV penalty has the drawback of creating blocky effects in the reconstructed images. To overcome the limitations of TV, in this paper we firstly introduce the structure tensor total variation (STV1) penalty into SIR framework for low dose CT image reconstruction. Then, an accelerated fast iterative shrinkage thresholding algorithm (AFISTA) is developed to minimize the objective function. The proposed AFISTA reconstruction algorithm was evaluated using numerical simulated low dose projection based on two CT images and realistic low dose projection data of a sheep lung CT perfusion. The experimental results demonstrated that our proposed STV1-based algorithm outperform FBP and TV-based algorithm in terms of removing noise and restraining blocky effects.

20.
FASEB J ; 34(4): 5061-5076, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32043638

RESUMO

Recently, interest in using whole food-derived mixtures to alleviate chronic metabolic syndrome through potential synergistic interactions among different components is increasing. In this study, the effects and mechanisms of tuna meat oligopeptides (TMOP) on hyperuricemia and associated renal inflammation were investigated in mice. Dietary administration of TMOP alleviated hyperuricemia and renal inflammation phenotypes, reprogramed uric acid metabolism pathways, inhibited the activation of NLRP3 inflammasome and TLR4/MyD88/NF-κB signaling pathways, and suppressed the phosphorylation of p65-NF-κB. In addition, TMOP treatments repaired the intestinal epithelial barrier, reversed the gut microbiota dysbiosis and increased the production of short-chain fatty acids. Moreover, the antihyperuricemia effects of TMOP were transmissible by transplanting the fecal microbiota from TMOP-treated mice, indicating that the protective effects were at least partially mediated by the gut microbiota. Thus, for the first time, we clarify the potential effects of TMOP as a whole food derived ingredient on alleviating hyperuricemia and renal inflammation in mice, and additional efforts are needed to confirm the beneficial effects of TMOP on humans.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA