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1.
BMC Plant Biol ; 21(1): 103, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33602134

RESUMO

BACKGROUND: The overall genetic distribution and divergence of cloned genes among bread wheat varieties that have occurred during the breeding process over the past few decades in Ningxia Province, China, are poorly understood. Here, we report the genetic diversities of 44 important genes related to grain yield, quality, adaptation and resistance in 121 Ningxia and 86 introduced wheat cultivars and advanced lines. RESULTS: The population structure indicated characteristics of genetic components of Ningxia wheat, including landraces of particular genetic resources, introduced varieties with rich genetic diversities and modern cultivars in different periods. Analysis of allele frequencies showed that the dwarfing alleles Rht-B1b at Rht-B1 and Rht-D1b at Rht-D1, 1BL/1RS translocation, Hap-1 at GW2-6B and Hap-H at Sus2-2B are very frequently present in modern Ningxia cultivars and in introduced varieties from other regions but absent in landraces. This indicates that the introduced wheat germplasm with numerous beneficial genes is vital for broadening the genetic diversity of Ningxia wheat varieties. Large population differentiation between modern cultivars and landraces has occurred in adaptation genes. Founder parents carry excellent allele combinations of important genes, with a higher number of favorable alleles than modern cultivars. Gene flow analysis showed that six founder parents have greatly contributed to breeding improvement in Ningxia Province, particularly Zhou 8425B, for yield-related genes. CONCLUSIONS: Varieties introduced from other regions with rich genetic diversity and landraces with well-adapted genetic resources have been applied to improve modern cultivars. Founder parents, particularly Zhou 8425B, for yield-related genes have contributed greatly to wheat breeding improvement in Ningxia Province. These findings will greatly benefit bread wheat breeding in Ningxia Province as well as other areas with similar ecological environments.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33527627

RESUMO

In this study, we developed a superstrong and reversible adhesive, which can possess a high bonding strength in the "adhesive" state and detach with the application of heating. An ionic crystal (IC) gel, in which an IC was immobilized within a soft-polymer matrix, were synthesized via in situ photo-crosslinking of a precursor solution composed of N, N-dimethyl acrylamide (DMAA) and a melted IC. The obtained IC gel is homogenous and transparent at melt point. When cooled to the phase transition temperature of the IC, the gel turns into the adhesive with the adhesion strength of 5.82 MPa (on glasses), due to the excellent wetting of melted gel and a thin layer of crystalline IC with high cohesive strength formed on the substrates. The synergistic effects between IC, polymer networks and substrates were investigated by solid state 1H NMR and molecular dynamics simulation. Such an adhesive layer is reversable and can be detached by heating and subsequent re-adhesion via cooling. This study proposed the new design of removable adhesives, which can be used in dynamic and complex environments.

3.
Neurobiol Dis ; 152: 105302, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33609640

RESUMO

Both spinophilin (SPN, also known as neurabin 2) and Rac1 (a member of Rho GTPase family) are believed to play key roles in dendritic spine (DS) remodeling and spinal nociception. However, how SPN interacts with Rac1 in the above process is unknown. Here, we first demonstrated natural existence of SPN-protein phosphatase 1-Rac1 complex in the spinal dorsal horn (DH) neurons by both double immunofluorescent labeling and co-immunoprecipitation, then the effects of SPN over-expression and down-regulation on mechanical and thermal pain sensitivity, GTP-bound Rac1-ERK signaling activity, and spinal DS density were studied. Over-expression of SPN in spinal neurons by intra-DH pAAV-CMV-SPN-3FLAG could block both mechanical and thermal pain hypersensitivity induced by intraplantar bee venom injection, however it had no effect on the basal pain sensitivity. Over-expression of SPN also resulted in a significant decrease in GTP-Rac1-ERK activities, relative to naive and irrelevant control (pAAV-MCS). In sharp contrast, knockdown of SPN in spinal neurons by intra-DH pAAV-CAG-eGFP-U6-shRNA[SPN] produced both pain hypersensitivity and dramatic elevation of GTP-Rac1-ERK activities, relative to naive and irrelevant control (pAAV-shRNA [NC]). Moreover, knockdown of SPN resulted in increase in DS density while over-expression of it had no such effect. Collectively, SPN is likely to serve as a regulator of Rac1 signaling to suppress DS morphogenesis via negative control of GTP-bound Rac1-ERK activities at postsynaptic component in rat DH neurons wherein both mechanical and thermal pain sensitivity are controlled.

4.
J Dairy Sci ; 2021 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-33551160

RESUMO

Streptococcus agalactiae is a contagious pathogen that causes bovine mastitis worldwide, resulting in considerable economic losses. In this study, we isolated 42 S. agalactiae strains in 379 milk samples from cows with subclinical mastitis on 15 dairy farms in 12 Chinese provinces. Analysis based on capsular typing and multilocus sequence typing, combined with patterns of virulence gene scanning and antimicrobial resistance, identified the lineages and populations of the isolates. We grouped the 42 isolates into 7 sequence types belonging to 6 clonal complexes, mainly CC103 (31/42 isolates; 73.8%). We identified an ST-23 strain named Sa 129 for the first time on Chinese dairy farms-this strain is usually associated with human isolates. Capsular types Ia and II were predominant in capsular typing. The prevalence of virulence profile 1 (bibA, cfb, cspA, cylE, fbsA, fbsB, hylB, and pavA) was 64.3%, and represented the main trend in China. With respect to antimicrobial resistance, most isolates were susceptible to ß-lactams, rifamycin, glycopeptides, and oxazolidone; resistance to several antimicrobial agents, including lincomycin, clindamycin, and doxycycline, varied in 4 different regions. Our research provides a profile for the molecular epidemiology, multilocus sequence typing, antimicrobial resistance, and virulence gene clustering of S. agalactiae, and may be beneficial for the clinical monitoring, prevention, and control of mastitis in dairy cattle.

5.
Mol Med Rep ; 23(2): 1, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33300066

RESUMO

The purpose of the present study was to determine the biological function and associated regulatory mechanism of small nucleolar RNA host gene 14 (SNHG14) in childhood acute myeloid leukaemia (AML). SNHG14 expression was measured via RT­qPCR in bone marrow tissues from 57 patients with AML and 57 healthy donors. The clinicopathological features of AML patients with low and high SNHG14 expression were analysed. AML cell viability and apoptosis were assessed using MTT and flow cytometry analyses. The starBase online database, and RNA­binding protein immunoprecipitation and dual luciferase reporter gene assays were employed to analyse the interactions among SNHG14, microRNA (miR)­193b­3p and MCL1 apoptosis regulator BCL2 family member (MCL1). SNHG14 was found to be overexpressed in the bone marrow tissues of patients with AML. The French­American­British classification and cytogenetics were significantly different between patients with high and low expression of SNHG14. Silencing SNHG14 decreased AML cell proliferation and facilitated apoptosis. SNHG14 functioned as a sponge for miR­193b­3p, and miR­193b­3p decreased the viability and accelerated the apoptosis rate of AML cells. In addition, miR­193b­3p targeted MCL1. Furthermore, silencing SNHG14 resulted in the sponging of miR­193b­3p to regulate cell viability, apoptosis, and MCL1 expression in AML. SNHG14 silencing decreased the viability and promoted apoptosis of AML cells by modulating the miR­193b­3p/MCL1 axis.

6.
Invest Ophthalmol Vis Sci ; 61(14): 9, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33275652

RESUMO

Purpose: The identification of target pathways to block excessive angiogenesis while simultaneously restoring physiological vasculature is an unmet goal in the therapeutic management of ischemic retinopathies. pNaKtide, a cell-permeable peptide that we have designed by mapping the site of α1 Na/K-ATPase (NKA)/Src binding, blocks the formation of α1 NKA/Src/reactive oxygen species (ROS) amplification loops and restores physiological ROS signaling in a number of oxidative disease models. The aim of this study was to evaluate the importance of the NKA/Src/ROS amplification loop and the effect of pNaKtide in experimental ischemic retinopathy. Methods: Human retinal microvascular endothelial cells (HRMECs) and retinal pigment epithelium (ARPE-19) cells were used to evaluate the effect of pNaKtide on viability, proliferation, and angiogenesis. Retinal toxicity and distribution were assessed in those cells and in the mouse. Subsequently, the role and molecular mechanism of NKA/Src in ROS stress signaling were evaluated biochemically in the retinas of mice exposed to the well-established protocol of oxygen-induced retinopathy (OIR). Finally, pNaKtide efficacy was assessed in this model. Results: The results suggest a key role of α1 NKA in the regulation of ROS stress and the Nrf2 pathway in mouse OIR retinas. Inhibition of α1 NKA/Src by pNaKtide reduced pathologic ROS signaling and restored normal expression of hypoxia-inducible factor 1-α/vascular endothelial growth factor (VEGF). Unlike anti-VEGF agents, pNaKtide did promote retinal revascularization while inhibiting neovascularization and inflammation. Conclusions: Targeting α1 NKA represents a novel strategy to develop therapeutics that not only inhibit neovascularization but also promote physiological revascularization in ischemic eye diseases.

7.
Front Oncol ; 10: 545862, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33163395

RESUMO

Background: In differentiating indeterminate pulmonary nodules, multiple studies indicated the superiority of deep learning-based computer-assisted diagnosis system (DL-CADx) over conventional double reading by radiologists, which needs external validation. Therefore, our aim was to externally validate the performance of a commercial DL-CADx in differentiating benign and malignant lung nodules. Methods: In this retrospective study, 233 patients with 261 pathologically confirmed lung nodules were enrolled. Double reading was used to rate each nodule using a four-scale malignancy score system, including unlikely (0-25%), malignancy cannot be completely excluded (25-50%), highly likely (50-75%), and considered as malignant (75-100%), with any disagreement resolved through discussion. DL-CADx automatically rated each nodule with a malignancy likelihood ranging from 0 to 100%, which was then quadrichotomized accordingly. Intraclass correlation coefficient (ICC) was used to evaluate the agreement in malignancy risk rating between DL-CADx and double reading, with ICC value of <0.5, 0.5 to 0.75, 0.75 to 0.9, and >0.9 indicating poor, moderate, good, and perfect agreement, respectively. With malignancy likelihood >50% as cut-off value for malignancy and pathological results as gold standard, sensitivity, specificity, and accuracy were calculated for double reading and DL-CADx, separately. Results: Among the 261 nodules, 247 nodules were successfully detected by DL-CADx with detection rate of 94.7%. Regarding malignancy rating, DL-CADx was in moderate agreement with double reading (ICC = 0.555, 95% CI 0.424 to 0.655). DL-CADx misdiagnosed 40 true malignant nodules as benign nodules and 30 true benign nodules as malignant nodules with sensitivity, specificity, and accuracy of 79.2, 45.5, and 71.7%, respectively. In contrast, double reading achieved better performance with 16 true malignant nodules misdiagnosed as benign nodules and 26 true benign nodules as malignant nodules with sensitivity, specificity, and accuracy of 91.7, 52.7, and 83.0%, respectively. Conclusion: Compared with double reading, DL-CADx we used still shows inferior performance in differentiating malignant and benign nodules.

8.
Int J Biol Macromol ; 2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33166556

RESUMO

As a heparin analogue, sulfonated chitosan (SCS) has been confirmed to have similar structure and properties to heparin which is shown to be a linker molecule having specific binding sites with collagen fibrils. In this study, the effects of a varying concentration of SCS on the self-assembly process of type I collagen were investigated. The study on intermolecular interaction between collagen and SCS was carried out via using ultraviolet-visible (UV-vis) spectrophotometry and circular dichroism (CD) spectroscopy. The addition of SCS did not disrupt the triple helix conformation of collagen. However, the decreased value of Rpn showed that the SCS, to some extent, influenced the percentage of triple helix conformation. The turbidity measurements revealed that the self-assembly rate was increased in the presence of a low concentration of SCS whereas decreased with further increasing the SCS concentration. The observation of microstructure via scanning electron microscopy (SEM) and atomic force microscopy (AFM) exhibited the characteristic D-periodicity, indicating that the presence of SCS did not disrupt the self-assembly nature of collagen. Moreover, the addition of SCS facilitated the lateral aggregation of fibrils, leading to the formation of larger fibrils. The rheological analysis showed that the gelation time of collagen was prolonged with increasing the concentration of SCS, in support of a longer lag-phase duration detected in turbidimetric measurements. We expect that valuable data would be provided in this study for further developing of ECM analogues, and propitious performances could be endowed to these biomimetic materials after SCS incorporation.

9.
Eur J Pharmacol ; : 173734, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33220272

RESUMO

Perioperative neurocognitive disorder (PND) is a common complication of elderly patients after surgery and lacks effective prevention and treatment measures. We investigated the effect and mechanism of gastrodin (GAS), a natural plant ingredient, on postoperative cognition induced by laparotomy in aged mice. Male aged (18 months) mice were subjected to laparotomy and orally treated with GAS (25, 50, and 100 mg/kg) 3 weeks before surgery and 1 week after surgery. In addition, some male aged (18 months) mice were subjected to viral vector or GSK-3ß expression virus injection followed by laparotomy with or without 100 mg/kg GAS treatment. GAS improved learning and memory in aged mice after surgery. Surgery increased the levels of pro-inflammatory factors (TNF-α, IL-1ß and IL-6) and decreased the level of an anti-inflammatory factor (IL-10) in the mouse hippocampus, and these changes were reversed by GAS treatment. GAS also suppressed the activation of microglia. GAS inhibited the phosphorylation of GSK-3ß and Tau. Furthermore, surgery induced more serious cognitive dysfunction, inflammatory factors, activation of microglia, and phosphorylation of GSK-3ß and Tau in GSK-3ß overexpressing aged mice. The improvement of learning and memory, the reduction of inflammation and microglia activation, and the suppression of GSK-3ß and Tau phosphorylation by GAS were prevented when GSK-3ß was overexpressed in aged mice subjected to surgery. Our finding suggested that GAS exerts neuroprotective effects in aged mice subjected to laparotomy by suppressing neuroinflammation and GSK-3ß and Tau phosphorylation. Thus, these findings suggest that GAS may be a promising agent for PND.

10.
Oncol Lett ; 20(6): 385, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33154782

RESUMO

5-Fluorouracil (5-FU)-based chemotherapy is the first-line option for patients with advanced colorectal cancer (CRC). However, the development of chemoresistance is the primary cause of treatment failure. Halofuginone (HF), a small molecule alkaloid derived from febrifugine, has been demonstrated to exert strong anti-proliferative effects. However, to the best of our knowledge, whether HF inhibits the progression of 5-FU-resistant human CRC HCT-15/FU cells, and the underlying mechanisms, remain unknown. In the present study, the effects of HF on HCT-15/FU cells were assessed in vitro. The results revealed that HF inhibited HCT-15/FU cell viability as demonstrated by the MTT and colony formation assays. Following treatment of HCT-15/FU cells with HF, the migratory and invasive capacities of the cells were significantly decreased. MicroRNA (miRNA/miR)-sequencing data, subsequent miRNA trend analysis and reverse transcription-quantitative PCR all demonstrated that miR-132-3p expression was increased following treatment with HF in a dose-dependent manner. Western blot analysis indicated that following treatment with HF, the expression levels of proteins associated with proliferation, invasion and metastasis in cells were markedly downregulated. These results suggested that HF inhibited the proliferation, invasion and migration of HCT-15/FU cells by upregulating the expression levels of miR-132-3p. Therefore, miR-132-3p may serve as a molecular marker, which may be used to predict CRC resistance to 5-FU, and HF may serve as a novel clinical treatment for 5-FU-resistant CRC.

11.
Sensors (Basel) ; 20(22)2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-33203079

RESUMO

Spacecraft formation flying (SFF) in highly elliptical orbit (HEO) has attracted much attention since many applications in space explore, while precise guidance navigation and control (GNC) technology, especially precise ranging, conducted the basis of success for such SFF missions. In this paper, we introduced a novel K band microwave ranging (MWR) equipment that aimed for the on-orbit verification of submillimeter level precise ranging technology in future HEO SFF missions. The ranging technique is a synchronous dual one-way ranging (DOWR) microwave phase accumulation system, which achieved tens of microns of ranging accuracy in laboratory environment. Detailed design and development process of MWR equipment are provided, with ranging error sources analyzed, and relative orbit dynamic models for HEO formation scenes are given with real perturbations considered. Moreover, an adaptive Kalman filter algorithm is introduced for SFF relative navigation design, incorporating with process noise uncertainty. The performance of SFF relative navigation while using MWR are tested in a hardware in loop (HIL) simulation system within a high precision six degree of freedom (6-DOF) moving platform. The final range estimation errors from MWR using adaptive filter are less than 35 µm m and 8.5 µm/s for range rate, which demonstrated the promising accuracy for future HEO formation mission applications.

12.
J Environ Qual ; 2020 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-33241881

RESUMO

In this study, carboxyl functionalized multiwall CNTs (c-MWCNTs) in plant (lettuce) tissues were quantitatively analyzed with programmed thermal analysis (PTA) coupled with a sequential digestion. PTA analysis evidenced a linear relationship between c-MWCNT-bound carbon and elemental carbon (EC) detected. A detection limit of 114 - 708 µg C/g plant tissues (dry mass) was achieved for analysis of c-MWCNTs. The method was demonstrated using the tissues of lettuce cultured hydroponically for three weeks with c-MWCNTs at an exposure of 10 and 20 µg/mL. This quantitative analysis can be used to provide insights into CNT exposure through agricultural products and promote its sustainable application. This article is protected by copyright. All rights reserved.

13.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(11): 1543-1549, 2020 Nov 30.
Artigo em Chinês | MEDLINE | ID: mdl-33243741

RESUMO

OBJECTIVE: To assess the effect of transnasal humidified rapid-insufflation ventilatory exchange (THRIVE) on gastric insufflation during general anesthesia induction in obese patients. METHODS: Ninety obese patients (BMI 30-39.9 kg/m2) undergoing laparoscopic cholecystectomy under general anesthesia were randomized into 3 groups (n=30) to receive facemask pre- oxygenation followed by face mask ventilation (FMV) after administration of anesthetics (Group M), oxygenation with THRIVE (Group T), or pre-oxygenation with facemask combined with THRIVE followed continuous oxygenation with both FMV and THRIVE after administration of anesthetics (Group M+T). The patients in the latter two groups received continuous oxygen via THRIVE during tracheal intubation. All the patients received real-time ultrasound monitoring of the gastric antrum, and positive gastric insufflation (GI+) was defined by the presence of comet-tail artifacts. The cross-sectional area of the gastic antrum (CSA-GA) was measured by ultrasound before and after pre-oxygenation and after intubation. The patients' SpO2, PaO2, and PaCO2 at admission (T1), 5 min after pre-oxygenation (T2), 5 min after medication (T3), and immediately after intubation (T4) were recorded, and the incidence of postoperative adverse events was assessed. RESULTS: The incidence of gastric insufflation was significantly higher in Group M and Group M+T than in Group T (P < 0.05). The CSA-GA was significantly greater at T4 than at T1 in Group M and Group M+T and in their GI+s ubgroups. The GI+ subgroups in Group M and Group M+ T had significantly larger CSA-GA at T4 than the GI- subgroups (P < 0.05). CSA-GA did not vary significantly during anesthesia induction in Group T (P>0.05). The incidence of grade Ⅰ gastric distension was lower but grade Ⅱ gastric distention was higher in Group M and Group M+T than in Group T (P < 0.05). Group M showed significantly greater variations of PaO2 at T3 and T4 than Group T and Group M+T (P < 0.05). CONCLUSIONS: Ultrasound monitoring of the comet tail sign and the changes of CSA-GA in the gastric antrum is feasible and reliable for detecting gastrointestinal airflow, and in obese patients, the application of THRIVE for induction of anesthesia can ensure the oxygenation level without further increasing gastric insufflation.

14.
Environ Sci Technol ; 54(22): 14235-14245, 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33108869

RESUMO

Intermediate volatility and semivolatile organic compounds (IVOC/SVOC) are important precursors of secondary organic aerosol (SOA) while SVOC is an important contributor to primary organic aerosol (POA). However, combustion emissions data for volatility classes are limited. This study reports the gas and particle emissions that were sampled with various dilution factors from a sewage sludge incinerator burning fuel oil. Volatility distributions were determined using measurements from online mass spectrometry and offline organic compound analyses. In the low volatility organic compound (LVOC) to IVOC range, volatility bins with organic saturation concentrations of 10-100 µg m-3 were most abundant, which was due to organic acids generated from sludge burning. Organic aerosol (OA) emission factors (EFOA) increased 1.4 times after cooling to ambient temperatures in comparison to those of the samples from the hot stack. Upon further isothermal dilution at 25 °C, the EFOA decreased while organic gas phase EFs increased with increasing dilution. Phase partitioning in volatility bins with saturation concentrations of 10-100 µg m-3 was sensitive to isothermal dilution that influenced the EFs. Therefore, gas- and particle-phase measurements alone cannot constrain EFs for these volatility classes. Low dilution factors may overestimate the particle phase and underestimate the gas phase EFs compared with real-world emission conditions.

15.
J Med Econ ; : 1-5, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33081555

RESUMO

Electronic health records (EHRs) can define real world patient populations with high levels of clinical specificity, potentially addressing some of the shortcomings of other types of real world data (RWD) when informing decisions about the comparative effectiveness of medical technologies. An important but under-recognized concern for EHR-derived RWD, however, is that the rich clinical data permits creation of very homogenous subpopulations from the larger group of eligible patients, thereby reducing the representativeness of the cohort relative to clinical practice. In this article, we discuss the tradeoffs between choosing clinical specificity versus representativeness in population sampling for comparative effectiveness research. Using EHR-derived RWD, we provide an example in non-small cell lung cancer to illustrate the concepts, showing wide variation in outcomes among potential comparator cohorts. We close with several recommendations for selecting comparator populations from EHRs that address the balance between matching clinical guidelines and capturing practice variability in comparative effectiveness research.

16.
Int J Biol Macromol ; 165(Pt A): 1164-1174, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33038398

RESUMO

Injectable hydrogels are of great interest in tissue engineering, and those incorporating hydroxyapatite (HA) are especially acclaimed in the application of bone repair. Synthetic micro-HA were generally used for this purpose and in some cases, surface modification of HA was further applied to improve the interfacial compatibility of rigid inorganic HA with soft organic matrix. In this study, the injectable hydrogels based on oxidized alginate hybrid HA nanoparticles and carboxymethyl chitosan were achieved via Schiff base reaction. Physicochemical characterization confirmed that oxidized HA/Alg hybrids (OHAH) were successfully prepared. Rheological measurements verified the formation of hydrogels based on the dynamic imine bonding, and the gelation time showed a negative correlation to the concentration and oxidation time of OHAH, while the storage moduli exhibited a positive correlation. The self-healing property of these hydrogels was validated by the splicing experiments and rheological experiments. The lyophilized hydrogels showed porous structures with numerous HA nanoparticles distributed on the surface of pore wall. MTT assays and live/dead staining of cell experiments confirmed the cytocompatibility of these hydrogels. The injectable hydrogels with self-healing and tunable gelling properties were ingeniously prepared with functionalized alginate-mediated HA hybrid nanoparticles, and these hydrogels are promising for applications in bone tissue engineering.

17.
Thorac Cancer ; 11(12): 3596-3598, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33063939

RESUMO

Here, we present a case of a distal-type bronchiolar adenoma (BA) of the lung. BAs are benign lung tumors characterized by nodular proliferation of bilayered bronchiolar-type epithelium with a continuous layer of basal cells. This patient underwent S3 segmentectomy following detection by computed tomography (CT) scan of a gradually enlarging ground-glass nodule (GGO) over a five month period. Nodule morphology and immunophenotype were consistent with those of distal-type BA of the lung. An epidermal growth factor receptor (EGFR) exon 21 p.L858R missense mutation was identified which, to the best of our knowledge, is the first case to be reported of a common gene mutation associated with non-small cell lung cancer (NSCLC) being found in a BA lesion. Following surgery, the patient remains relapse-free. KEY POINTS: SIGNIFICANT FINDINGS OF THE STUDY: Pathological assessment of a lung nodule confirmed a papillary tumor with a double-layered cell structure, less than typical cytoplasm, and a mixture of ciliated columnar and globular cells, consistent with a distal-type bronchiolar adenoma. WHAT THIS STUDY ADDS: This is the first report of an EGFR exon 21 p.L858R mutation in a bronchiolar adenoma.

18.
Medicine (Baltimore) ; 99(40): e22596, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33019479

RESUMO

BACKGROUND: The pathophysiologic of vascular malformations is still unclear, and the treatment of vascular malformations is a challenge. With improvement in the understanding of pathogenesis of vascular malformations, sirolimus has been a promising and effective treatment. As so far, there is absent convincing evidence to confirm the efficacy of sirolimus for vascular malformations. The purpose of this study was to evaluate the effectiveness and safety of sirolimus in the treatment of vascular malformations. METHODS: The literatures about the management of vascular malformations with sirolimus would be searched from databases of MEDLINE, EMBASE, PubMed, Web of Science, Clinicaltrials.org., Cochrane Library, China Biology Medicine Database (CBM), Wan Fang Database, China National Knowledge Infrastructure Database (CNKI), and VIP Science Technology Periodical Database. We will search each database from inception or 1995 to August 20, 2020. Two researchers worked independently on literature selection, data extraction and quality assessment. The efficacy and safety of sirolimus in the treatment of vascular malformations were the main outcomes. Adverse events after sirolimus were evaluated as the secondary outcomes. The included studies will be analyzed by Review Manager 5.3. If the results are applicable, meta-analysis would also be performed. RESULTS: The study will evaluate the efficacy and safety of sirolimus in the treatment of vascular malformations based on current evidence. CONCLUSION: The conclusion of this study will provide more reliable, evidence-based data for the use of sirolimus in the treatment of vascular malformations. PROSPERO REGISTRATION NUMBER: CRD42020167881.


Assuntos
Imunossupressores/uso terapêutico , Sirolimo/uso terapêutico , Malformações Vasculares/tratamento farmacológico , Gerenciamento de Dados , Feminino , Humanos , Imunossupressores/efeitos adversos , Masculino , Projetos de Pesquisa , Segurança , Sirolimo/efeitos adversos , Serina-Treonina Quinases TOR/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo , Resultado do Tratamento , Malformações Vasculares/fisiopatologia
19.
ACS Cent Sci ; 6(9): 1497-1506, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32999925

RESUMO

Enzyme immobilization in metal-organic frameworks (MOFs) as a promising strategy is attracting the interest of scientists from different disciplines with the expansion of MOFs' development. Different from other traditional host materials, their unique strengths of high surface areas, large yet adjustable pore sizes, functionalizable pore walls, and diverse architectures make MOFs an ideal platform to investigate hosted enzymes, which is critical to the industrial and commercial process. In addition to the protective function of MOFs, the extensive roles of MOFs in the enzyme immobilization are being well-explored by making full use of their remarkable properties like well-defined structure, high porosity, and tunable functionality. Such development shifts the focus from the exploration of immobilization strategies toward functionalization. Meanwhile, this would undoubtedly contribute to a better understanding of enzymes in regards to the structural transformation after being hosted in a confinement environment, particularly to the orientation and conformation change as well as the interplay between enzyme and matrix MOFs. In this Outlook, we target a comprehensive review of the role diversities of the host matrix MOF based on the current enzyme immobilization research, along with proposing an outlook toward the future development of this field, including the representatives of potential techniques and methodologies being capable of studying the hosted enzymes.

20.
Micromachines (Basel) ; 11(10)2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32992689

RESUMO

Platelet separation and purification are required in many applications including in the detection and treatment of hemorrhagic and thrombotic diseases, in addition to transfusions and in medical research. In this study, platelet separation was evaluated using a novel zigzag microchannel fluidic device while leveraging a dielectrophoresis (DEP) electric field using the COMSOL multiphysics software package and additional experimentation. The zigzag-shaped microchannel was superior to straight channel devices for cell separation because the sharp corners reduced the required horizontal distance needed for separation and also contributed to an asymmetric DEP electric field. A perfect linear relationship was observed between the separation distance and the corner angles. A quadratic relationship (R2 = 0.99) was observed between the driving voltage and the width and the lengths of the channel, allowing for optimization of these properties. In addition, the voltage was inversely proportional to the channel width and proportional to the channel length. An optimal velocity ratio of 1:4 was identified for the velocities of the two device inlets. The proposed device was fabricated using laser engraving and lithography with optimized structures including a 0.5 mm channel width, a 120° corner angle, a 0.3 mm channel depth, and a 17 mm channel length. A separation efficiency of 99.4% was achieved using a voltage of 20 V and a velocity ratio of 1:4. The easy fabrication, lower required voltage, label-free detection, high efficiency, and environmental friendliness of this device make it suitable for point-of-care medicine and biological applications. Moreover, it can be used for the separation of other types of compounds including lipids.

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