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1.
Front Biosci (Elite Ed) ; 12: 113-125, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31585873

RESUMO

We investigated coronary heart disease (CHD) and cardiovascular disease (CVD) event rates in a diverse population with a coronary artery calcium score (CACS) of 0 and the role of CACS in the detection of subclinical noncalcified atherosclerotic plaque. A total of 15,884 participants in five studies were included in this meta-analysis. Hazard ratios (HRs) with 95% confidence intervals (95% CIs) were calculated. The results showed that CHD incidence significantly increased with increased CACS (HR=0.05, 95% CI 0.03-0.06, Z=5.82, P=0.002). The CHD rate was low and further increased with CACS of 101-300. With CACS >300, the CHD rate was highest. Similarly, CVD rate was low with CACS of 0, increased with CACS of 1-100 (HR=0.03, 95% CI 0.01-0.06, Z=1.66, P=0.096), and further increased with CACS of 101-300. With CACS >300, the CVD rate was highest. Clinical evidence indicated that the higher the CACS, the higher the CHD and CVD rates, while the CVD rate does not always decreased compared with CHD rate with the same CACS, especially with CACS of 0.

2.
Bioelectrochemistry ; 131: 107387, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31698179

RESUMO

Enhancing the self-buffering capacity is critical in the operation of bufferless microbial fuel cells (BLMFCs). Inorganic carbon (IC) is an ideal endogenous buffer, but its spontaneously accumulated concentration is insufficient to adjust anolyte pH. In this study, BLMFCs were operated with anaerobic sludge to enhance IC accumulation and increase anolyte pH. The accumulated IC concentration during a single running cycle was elevated from 8.3 mM to 12.5 mM, and anolyte pH remained above 7.5. The electric power output was significantly promoted from 332.2 mW·m-2 to 628.1 mW·m-2, and the coulombic efficiency (CE) slightly increased from 16.4% to 19.5%. Geobacter was the electro-active genus in the anode biofilms of the MFCs, and its relative abundance in the KCl-S anode biofilm increased from 0.2% to 5.75%. After continuous operation, the predominant genus of the anaerobic sludge had changed from Flavobacterium to Fusibacter.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31612268

RESUMO

Dichlorvos (DDVP) is widely applied in the agricultural industry, and its residues are considered hazardous to the environment. Microbial bioremediation is an innovative technology with the potential to mitigate such pollution. Trichoderma atroviride strain T23, a filamentous fungus, is very efficient at degrading DDVP. Therefore, we used DDVP as a model organophosphate pesticide to study the mechanism by which Trichoderma degrades organophosphate pesticides, with the aim of attaining a global understanding of the molecular mechanism of enzymatic degradation of organophosphate pesticides by beneficial fungi. DDVP can be biodegraded via two routes, and the primary one involves hydrolysis of the P-O bond, which can result in the production of the novel degradation intermediate trichloroethanol. TaPon1-like showed continuously high expression during 120 h, and deletion of the gene decreased the efficiency of P-O bond hydrolysis. The enzyme produced by TaPon1-like had a low Km for DDVP (0.23 mM) and a high kcat (204.3 s-1). The enzyme was able to hydrolyze broad substrates such as organophosphate oxons and lactone and maintain stable activity in a wide range of pH and temperature values. The TaPon1-like hydrolase played an important role in the first step of DDVP degradation by strain T23 and contributed to a comprehensive understanding of the mechanism of organophosphate pesticide degradation.

4.
Medicine (Baltimore) ; 98(39): e17322, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31574865

RESUMO

BACKGROUND: This study will aim to assess the effectiveness of the rehabilitation training (RT) combined acupuncture for the treatment of patients with neurogenic bladder (NB) secondary to the spinal cord injury (SCI). METHODS: We will conduct a comprehensive literature search from the following databases from the inceptions to the present with no language limitation: PUBMED, EMBASE, Cochrane Library, SinoMed, Web of Science, Allied and Complementary Medicine Database, VIP, WANGFANG, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure. Additionally, we will also search gray literature, including dissertations and conference proceedings. RevMan V.5.3 software will be used for the study selection, assessment of bias of bias, and data synthesis. RESULTS: This study will synthesize the available evidence of RT combined with acupuncture for NB secondary to SCI, including episodes of urinary incontinence, urinary retention, urinary tract infection, bladder overactivity, quality of life, and adverse events. CONCLUSION: This study will determine whether RT combined acupuncture is an effective and safety therapy for NB secondary to SCI. SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42019146127.


Assuntos
Terapia por Acupuntura/métodos , Traumatismos da Medula Espinal , Bexiga Urinaria Neurogênica , Humanos , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/reabilitação , Resultado do Tratamento , Bexiga Urinaria Neurogênica/etiologia , Bexiga Urinaria Neurogênica/terapia
5.
Nat Commun ; 10(1): 4209, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31527657

RESUMO

Natural killer/T cell lymphoma (NKTCL) is a rare and aggressive malignancy with a higher prevalence in Asia and South America. However, the molecular genetic mechanisms underlying NKTCL remain unclear. Here, we identify somatic mutations of GNAQ (encoding the T96S alteration of Gαq protein) in 8.7% (11/127) of NKTCL patients, through whole-exome/targeted deep sequencing. Using conditional knockout mice (Ncr1-Cre-Gnaqfl/fl), we demonstrate that Gαq deficiency leads to enhanced NK cell survival. We also find that Gαq suppresses tumor growth of NKTCL via inhibition of the AKT and MAPK signaling pathways. Moreover, the Gαq T96S mutant may act in a dominant negative manner to promote tumor growth in NKTCL. Clinically, patients with GNAQ T96S mutations have inferior survival. Taken together, we identify recurrent somatic GNAQ T96S mutations that may contribute to the pathogenesis of NKTCL. Our work thus has implications for refining our understanding of the genetic mechanisms of NKTCL and for the development of therapies.

8.
Taiwan J Obstet Gynecol ; 58(4): 501-504, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31307741

RESUMO

OBJECTIVE: This study aims to evaluate the value of the ultrasound-related scoring system on pregnant patients receiving assisted reproductive technology (IVF/ICSI) and early pregnancy outcome. MATERIALS AND METHODS: This prospective study included 208 pregnant women receiving assisted reproductive technology (IVF/ICSI). The following ultrasound parameters were measured: gestational sac size, the proportion of the embryo and gestational sac (embryo/gestational sac), yolk sac size, and fetal cardiac activity. The above data were assigned according to the ongoing pregnancy rate (up to 14 weeks), and the score increased parallel to the pregnancy rate. All patients were grouped according to their scores. RESULTS: Patients with a score of 4-5 had a low ongoing pregnancy rate of 14.29%, while patients with a score of 6-7 had an ongoing pregnancy rate of 55.56%. Surprisingly, patients with a score of 8-9 had an ongoing pregnancy rate of 97.22%. In addition, it was found that the ongoing pregnancy rate was 100% (36/36) in patients with a score of 9. Conversely, there was no ongoing pregnancy in patients with a score of 4. CONCLUSION: First, this scoring system is strongly associated with an ongoing pregnancy of over 14 weeks. Second, some reassurance can be given to patients with favorable ultrasound parameters, regardless of maternal age or previous pregnancy loss. Third, it would be meaningless to continue the pregnancy in patients with a score of 4, according to the scoring system. Fourth, patients without cardiac activity and embryos at days 33-35 after embryo transfer should discontinue the pregnancy, while patients with embryos should proceed with the pregnancy.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31195162

RESUMO

BACKGROUND & AIMS: There is controversy over whether use of non-vitamin K antagonist oral anticoagulants (NOACs) associates with increased risk of major gastrointestinal bleeding (GIB) compared with conventional therapies (such as vitamin K antagonists or anti-platelet agents). We performed a systematic review and meta-analysis of data from randomized controlled trials and high-quality real-world studies. METHODS: We performed a systematic search of the MEDLINE, EMBASE, Cochrane Library, and ClinicalTrials.gov Website databases (through Oct 12, 2018) for randomized controlled trials and high-quality real-world studies that reported major GIB events in patients given NOACs or conventional therapy. Relative risks (RRs) for randomized controlled trials and adjusted hazard ratios (aHRs) for real-world studies were calculated separately using random-effects models. RESULTS: We analyzed data from 43 randomized controlled trials (183,752 patients) and 41 real-world studies (1,879,428 patients). The pooled major rates of GIB for patients on NOACs (1.19%) vs conventional treatment (0.92%) did not differ significantly (RR from randomized controlled trials, 1.09; 95% CI, 0.91-1.31 and aHR from real-world studies, 1.02; 95% CI, 0.94-1.10; Pinteraction=.52). Rivaroxaban, but not other NOACs, was associated with an increased risk for major GIB (RR from randomized controlled trials, 1.39; 95% CI, 1.17-1.65 and aHR from real-world studies, 1.14; 95% CI, 1.04-1.23; Pinteraction = .06). Analyses of subgroups, such as patients with different indications, dosage, or follow-up time, did not significantly affect results. Meta-regression analysis failed to detect any potential confounding to impact the primacy outcome. CONCLUSIONS: In a systematic review and meta-analysis of data from randomized controlled trials and real-world studies, we confirmed that there is no significant difference in risk of major GIB between patients receiving NOACs vs conventional treatment. Rivaroxaban users had a 39% increase in risk for major GIB.

11.
Cancer Med ; 8(8): 3892-3904, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31124291

RESUMO

BACKGROUND: Natural Killer T-Cell Lymphoma (NKTCL) is a subtype of Non-Hodgkin's Lymphoma, and its morbidity is ranked the first of T-Cell Lymphoma. Hippo signaling pathway is involved in the pathogenesis of tumors. However, the role of Hippo signaling pathway in the oncogenesis of NKTCL still remains unclear. METHODS: The expressions of mammalian sterile 20-like kinase 1 (MST1) and Yes-associated protein (YAP) were investigated by RT-PCR and Western blotting. Cell viability was detected by MTT assays. Cell cycle and cell apoptosis were determined by flow cytometry. Cell proliferative capacity was detected by colony formation assay. Nude mice xenograft models were established and the tumor sections were analyzed by immunohistochemistry (IHC) staining. RESULTS: The expression of MST1 was significantly down-regulated in NKTCL tissues (n = 30) and cell lines, while the expression of YAP was significantly up-regulated, and the phosphorylation of YAP was inhibited. Overexpression of MST1, knockdown of YAP, or verteporfin (VP) treatment could inhibit cell proliferation, and promote cell cycle arrest and apoptosis in NKTCL cells, while knockdown of MST1 and overexpression of YAP promoted cell proliferation. Additionally, Bcl-2/Bax ratio and downstream effectors of Hippo signaling pathway (c-myc, survivin, cyclinD1, CTGF, and TEAD) were significantly decreased when MST1 was overexpressed and YAP was knocked down or after VP treatment. Furthermore, our mice model demonstrated that activation of Hippo signal pathway suppressed the tumorigenesis of NKTCL. CONCLUSION: The activation of Hippo signal pathway via overexpressing MST1 or down-regulating YAP can inhibit the tumorigenesis of NKTCL.

12.
Fungal Biol ; 123(6): 448-455, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31126421

RESUMO

Maize stalk rot and ear rot, caused by Fusarium graminearum and Fusarium verticillioides, respectively, are major diseases that threaten the sustainable production of maize. In this study, an artificial inoculation assay demonstrated that the control efficacy of maize stalk rot and ear rot by Trichoderma asperellum granules were 49.83 % and 39.63 %, respectively. By high-throughput sequencing of maize plants, a total of 76 196 ITS1 sequences and 887 226 V3V4 16S rRNA sequences were analyzed and were grouped into 2934 fungal and 24 248 bacterial operational taxonomic units (OTUs), respectively. It revealed a significantly higher endophytic microbial abundance in the stem tissue of plants grown in T. asperellum-treated soil than in those grown in the control, with the largest increase observed in the basal stem section. In addition, the endophytic microbial diversity and corresponding control effects all gradually decreased from the basal to apical parts of the stem in plants grown in Trichoderma-treated soil, indicating that Trichoderma stimulated a more significant effect on the defense system in the basal section of the stalk than in the apical parts of plants. Furthermore, the accumulation of deoxynivalenol (DON) and fumonisin B1 (FB1) decreased in the stem and ear of maize grown in T. asperellum-treated soil.

13.
Sci Total Environ ; 676: 436-446, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31048173

RESUMO

In order to analyze the temporal-spatial distribution characteristics of PM2.5, PM10, SO2, NO2, CO and O3 in five cities and the potential sources of PM10 in southern Xinjiang during 2016, we collected one year officially released data for analysis. The average PM10, PM2.5, SO2, NO2, O3 and CO concentrations were 289 ±â€¯363, 99 ±â€¯106, 17 ±â€¯9, 29 ±â€¯11, 65 ±â€¯25 µg m-3 and 1.3 ±â€¯0.6 mg m-3 in southern Xinjiang in 2016, respectively. The air pollutants presented distinct seasonal and spatial distribution characteristics. During sandstorm process, the particulate matters (PM) concentrations increased abruptly, with the PM10 and PM2.5 maximum concentrations exceeding 1000 and 500 µg m-3 in each city. The backward trajectory results showed that the air masses in Akesu, Kurla, Hotan, Kashi and Atushi were mainly from the Bayingol Mongolian Autonomous Prefecture, Kyrgyzstan, Kizilesu Kirgiz Autonomous Prefecture and Taklimakan Desert (TD). In addition, TD was the main potential contributor to ambient PM10 in five cities during the dust season (DS), with a weighted potential source contribution function (WPSCF) > 0.9. While the trajectories of air masses from TD, Bayingol Mongolian Autonomous Prefecture, Urumqi-Changji Area and local emission were potential sources contributing to PM2.5 in these five cities during DS, with a WPSCF > 0.7. Moreover, the high weighted concentration weighted trajectory (WCWT) values were distributed in the Tarim basin, with PM10 > 700 µg m-3, however, the local emission and long distance transport contributed to the PM2.5 > 160 µg m-3 for five cities. This study comprehensively analyzes the pollution characteristics of air pollutants in five important cities in the southern margin of the Tarim Basin for the first time, and will provide an important reference basis for the prevention and control of air pollution in southern Xinjiang.

14.
Huan Jing Ke Xue ; 40(3): 1052-1061, 2019 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087951

RESUMO

To characterize the chemical composition of PM2.5 and the formation of pollution during a heavy pollution episode in the winter in a typical logistics city, PM2.5 samples were collected from December 2016 to January 2017 at six sampling sites, and the water-soluble ions, elements, and carbon components were determined. The results showed that the average concentration of PM2.5 was (145.2±87.8) µg·m-3 during the whole sampling period, of which 82% of daily average concentrations were above class Ⅱ of the national standards. The average concentrations of PM2.5 during the two heavy pollution episodes were (187.3±79.8) and (205.3±92.0) µg·m-3, which were 5.4 and 5.9 times, respectively, as high as class Ⅱ of the national standard. The results of the chemical composition of the PM2.5 showed that secondary water-soluble inorganic ions (SO42-, NO3-, and NH4+) were the main components of PM2.5 in winter (51.2% of PM2.5 mass concentration), followed by organic matter (OM, 23.8%), and mineral dust (12.7%). Combined with the change trend and accumulation rate of chemical components during the pollution episode, we discovered that the increasing of SNA and OM led to PM2.5 pollution in the first episode, while the growth of SNA caused the second pollution episode. This was further verified by the synchronous change of SOR, NOR, and the OC/EC ratio. PMF analysis indicated that mixed sources of secondary particulate matter and biomass combustion (50.0%), coal combustion (16.8%), vehicles (12.9%), fugitive dust (10.0%), industry (5.3%) and soil dust (5.0%), were the main sources of PM2.5 of Linyi city in the winter. Compared with the average concentration over the whole sampling period, the contribution of secondary particles during the two pollution episodes was significantly increased. This indicates that the formation and accumulation of secondary particulate matter under static and humid meteorological conditions were the main influencing factors during the heavy pollution episodes.

15.
Huan Jing Ke Xue ; 40(3): 1062-1070, 2019 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087952

RESUMO

To study the atmospheric PM2.5 pollution characteristics and sources in heating and non-heating periods in Shenyang, 113 groups of effective PM2.5 samples were collected from January 29, 2015 to January 26, 2016, and the water-soluble ions, carbon constituents and elements in PM2.5 were tested. The results indicated that the average PM2.5 mass concentration in Shenyang during the sampling period was 66 µg·m-3. Among the sampled PM2.5 concentrations, 31.0% exceeded the daily value of the secondary standard limit of the Chinese National Ambient Air Quality Standard (75 µg·m-3). The average concentration and over-standard rate of PM2.5 in the heating period (90 µg·m-3, 68.6%) was higher than that of the non-heating period (51 µg·m-3, 31.4%). The concentrations of the 21 elements (except Mg, Ti, Ca, Fe, and Si), water-soluble ions (except Ca2+), OC, and EC were all higher in the heating period than in the non-heating period. The ratio of[NO3-]/[SO42-]showed that the influence of moving source increased obviously in the non-heating period, and fixed source was still the main contributor in the heating period. The water-soluble ions were the result of the interaction of fixed source and moving source. The NOR and SOR analyses showed that the secondary conversion of NOx was weak, and the secondary conversion of SO2 was obvious, especially in the non-heating period. The enrichment factor showed that the elements with high EF value mainly came from coal burning, traffic pollution, and industrial emissions. The reconstructed PM2.5 masses were highly correlated with the measured ones. The main constituents of PM2.5 in both heating and non-heating seasons were organic matter (28.0%, 23.1%), mineral dust (14.5%, 26.0%), and sulfate (15.1%, 19.9%), and PM2.5 was mainly affected by the secondary particles, combustion sources and dust sources.

16.
Sci Total Environ ; 676: 11-17, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31029896

RESUMO

Anolyte acidification is inevitable in the operation of buffer-free microbial fuel cells (MFCs), which restricts the proliferation and metabolism of electroactive bacteria, and results in electric-power deterioration. The anodic metabolic end-products, inorganic carbons (IC), which are composed of H2CO3 (dissolved CO2), HCO3-, and CO32-, are ideal endogenous buffers, whereas the naturally accumulated IC are far from enough to prevent anolyte acidification. In this work, different volume ratios of the anolytes (10%, 30%, and 50%) were recycled to increase the IC concentrations of the single-chamber air-cathode buffer-free MFCs. Under anolyte recycling running mode, IC accumulation agreed with the SGompertz model and the fitting IC-asymptotic concentrations (ICAC) grew exponentially to 18.5 mM, 24.4 mM, and 32.8 mM as the anolyte recycling ratio increased from 10% to 30% and 50%. Self-buffering running can be realized when the anolyte recycling ratio exceeds 50% for the MFC feeding on 1 g·L-1 of acetate. The electric power for the 50% recycling scenario increased from the baseline control of 272.4 mW·m-2 to 628.5 mW·m-2. The coulombic efficiency (CE) was also apparently improved. This paper for the first time clarifies the accumulation law of endogenous IC buffers under anolyte partially recycling mode and their self-buffering effects.


Assuntos
Fontes de Energia Bioelétrica , Carbono/química , Bactérias , Tampões (Química) , Eletricidade , Eletrodos , Concentração de Íons de Hidrogênio
17.
Pulm Pharmacol Ther ; 56: 69-74, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30930172

RESUMO

Interstitial lung disease (ILD) is the most common pulmonary manifestation of Rheumatoid arthritis (RA) lung disease. The mechanism of RA-ILD remains obscure and more effective treatments are still needed. Resveratrol (RSV) a phytoalexin found with anti-inflammation and antioxidant activity. RSV has been reported to protect against RA. In current study, we evaluated the effects of RSV on RA-ILD and further explored the underlying mechanisms. We established the RA-ILD rat model by injecting Freund's complete adjuvant (FCA). After administration of RSV into RA-ILD rats, the disease parameters were assessed, inflammatory cytokines productions were analyzed, and the effects of RSV on JAK/STAT/RANKL were evaluated. Injection of FCA caused RA-ILD in rats, which had clear lung damage, fibrosis, and elevated pro-inflammatory cytokines in both serum and lung. RSV treatment significantly ameliorated the lung disease and prevented pro-inflammatory cytokines production. In addition, RSV inhibited JAK/STAT/RANKL signaling pathway in RA-ILD rats. RSV treatment alleviates RA-ILD in rats by inhibiting JAK/STAT/RANKL signaling pathway.

18.
Oncologist ; 2019 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-30952822

RESUMO

BACKGROUND: T-lymphoblastic lymphoma (T-LBL) is a highly aggressive neoplasm of lymphoblasts of T-cell origin. Although promising improvements have been recently achieved, one third of patients experience relapse or refractory T-LBL. Therefore, optimal strategies for identifying high-risk patients are urgently needed. MATERIALS AND METHODS: In the present study, 75 newly diagnosed adult patients (aged ≥15 years) with T-LBL were identified and the predictive value of complete blood count (CBC) abnormalities, including lymphocyte-monocyte ratio (LMR), neutrophil-lymphocyte ratio (NLR), and platelet-lymphocyte ratio (PLR) on clinical outcomes, was analyzed. RESULTS: Using the receiver operating characteristic curve to determine the best cutoff values based on survival, it was found that patients with T-LBL with LMR ≤2.8, NLR ≥3.3, and PLR ≥200 had both inferior progression-free survival (PFS) and inferior overall survival (OS), in which the differences were much more remarkable in the international prognostic index score 0-2 subgroup. In the multivariable analysis, NLR ≥3.3 together with age >40 years and central nervous system (CNS) involvement were identified to be independently associated with shortened PFS, whereas PLR ≥200 and CNS involvement were identified to be independent risk factors for OS. LMR, NLR, and PLR were integrated to generate a "CBC score" model, which well separated adult patients with T-LBL into three risk groups, and the 3-year OS was 84%, 53%, and 30% for low-, intermediate-, and high-risk patients, respectively. CONCLUSION: Overall, a "CBC score" model was initially promoted for stratification in adult patients with T-LBL using simple, widely available, and easy to interpret parameters in the largest adult T-LBL cohort to date. IMPLICATIONS FOR PRACTICE: Optimal strategies for identifying high-risk patients with T-lymphoblastic lymphoma (T-LBL) are urgently needed. In the largest adult T-LBL cohort to date, simple, inexpensive, widely available parameters were applied and revealed that patients with lymphocyte-monocyte ratio (LMR) ≤2.8, neutrophil-lymphocyte ratio (NLR) ≥3.3, and platelet-lymphocyte ratio (PLR) ≥200 had both inferior progression-free survival and inferior overall survival (OS), in which the differences were much more remarkable in the international prognostic index score 0-2 subgroup. LMR, NLR, and PLR were integrated to generate a "complete blood count score" model, in which the 3-year OS was 84%, 53%, and 30% for low-, intermediate-, and high-risk patients, respectively.

19.
BMJ Open ; 9(4): e024800, 2019 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-30944133

RESUMO

INTRODUCTION: Chou-Ling-Dan (CLD) (Laggerapterodonta) granules are an ethnic herbal medicine from Yunnan province of China. CLD granules have been used for the treatment of inflammatory conditions and feverish diseases in China, including seasonal influenza, but few evidence-based medicine (EBM) clinical studies have been conducted to assess its efficacy and safety in the treatment of influenza. Here, we performed an EBM clinical trial combining Western Chinese medicine and traditional Chinese medicine (TCM) evaluation systems to evaluate the efficacy and safety of CLD granules in the treatment of seasonal influenza. METHODS AND ANALYSIS: The study is designed as a multicentre, randomised, double-blinded, double-simulation, oseltamivir-controlled and placebo-controlled, parallel-design clinical trial. Eligible subjects (n=318) will be allocated after satisfying the criteria (Western medicine). Subjects will be randomised to receive CLD granules, oseltamivir, or a placebo for 5 days of treatment and with follow-up after treatment to record symptoms and signs and to collect pharyngeal/throat swabs and serum samples for detecting the virus and antibodies. At the same time, the syndrome differentiation criteria of TCM, such as tongue body, furred tongue and type of pulse, will be recorded as determined by doctors of both Western and Chinese medicine. Participants will be instructed to comply with the protocol and to keep a daily record of symptoms. The primary and secondary outcomes and safety indicators will be used to evaluate the efficacy and safety of CLD granules in the treatment of seasonal influenza based on both Western Chinese medicine and TCM evaluation systems. ETHICS AND DISSEMINATION: The CLD granules clinical trial will be conducted in accordance with the Declaration of Helsinki and Good Clinical Practice and has been approved by the Ethics Committee of the First Affiliated Hospital of Guangzhou Medical University. All participants must provide written informed consent. The results obtained will be disseminated at international medical conferences and in peer-reviewed publications. TRIAL REGISTRATION NUMBER: NCT02662426; Pre-results.

20.
Water Environ Res ; 91(8): 780-787, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30921491

RESUMO

In this study, a spontaneous electric field membrane bioreactor (SEF-MBR), equipped with the innovative Cu-nanowires conductive microfiltration membrane, was developed to achieve membrane fouling mitigation and high-quality effluent. The membrane fouling was significantly mitigated due to the presence of spontaneous electric field that the intensity of the spontaneous electric field in the established SEF-MBR was up to 0.073 V/cm. After over 2-month operation, the membrane flux of SEF-MBR was 2.1 times that of the control reactor. The thickness of fouling layer on the Cu-nanowires conductive membrane surface was about 80 µm, which was far thinner than that on the surface of commercial polyvinylidene fluoride (PVDF) membrane. Meanwhile, it was featured with the lower microbe density and extracellular polymeric substance (EPS) content. The effluent quality of SEF-MBR met the first-class discharge standards, and the removal rates were 94.5% for chemical oxygen demand (COD), 99.8% for NH 4 + - N , 78.5% for total nitrogen (TN), and 86.6% for total phosphorus (TP). The established system with the innovative Cu-nanowires conductive membrane showed a promising prospect for using the spontaneous electric field to mitigate membrane fouling and achieve high-quality effluent without extra power consumption. PRACTITIONER POINTS: The innovative Cu-NWs conductive microfiltration membrane was prepared. The spontaneous electric field in the novel SEF-MBR mitigated membrane fouling. The fouling layer of the novel SEF-MBR was thinner with lower microbe and EPS content. The effluent quality of the novel SEF-MBR met the first-class discharge standard.


Assuntos
Incrustação Biológica/prevenção & controle , Reatores Biológicos , Eletricidade , Membranas Artificiais , Purificação da Água , Cobre , Filtração , Nanofios
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