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1.
Talanta ; 203: 227-234, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31202330

RESUMO

In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment.

2.
Nanoscale ; 11(18): 9185-9193, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31038146

RESUMO

Recent advances in nanotechnology have developed a lot of opportunities for biological applications. In this work, multifunctional MoS2/AuNR nanocomposites with unique high NIR absorption were designed via combining MoS2 nanosheets and gold nanorods (AuNRs). The nanocomposites were synthesized through electrostatic self-assembly and showed high stability and good biocompatibility. Then they were used to modulate the aggregation of amyloid-ß peptides, destabilize mature fibrils under NIR irradiation, and eliminate Aß-induced ROS against neurotoxicity. The inhibition and destabilization effects were confirmed by Thioflavin T (ThT) fluorescence assay and transmission electron microscopy (TEM). Cell viability assay and ROS assay revealed that MoS2/AuNR nanocomposites could alleviate Aß-induced oxidative stress and cell toxicity. More importantly, both MoS2 nanosheets and AuNRs can be used as NIR photothermal agents, MoS2/AuNR nanocomposites have enhanced ability of disrupting Aß fibrils and improved cell viability by generating local heat under low power NIR irradiation. Our results provide new insights into the design of new multifunctional systems for the treatment of amyloid-related diseases.


Assuntos
Peptídeos beta-Amiloides/metabolismo , Raios Infravermelhos , Nanocompostos/química , Fragmentos de Peptídeos/metabolismo , Amiloide/química , Amiloide/toxicidade , Peptídeos beta-Amiloides/química , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Dissulfetos/química , Ouro/química , Humanos , Molibdênio/química , Nanocompostos/toxicidade , Nanotubos/química , Fragmentos de Peptídeos/química , Espécies Reativas de Oxigênio/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier
3.
J Colloid Interface Sci ; 539: 575-584, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30611053

RESUMO

Development of effective inhibitors toward Aß aggregation and reactive oxygen species (ROS) scavengers are of crucial therapeutic implications for Alzheimer's disease (AD). Herein, a novel agent with dual enzyme mimic activities has been fabricated as a multifunctional Aß fibrillation modulator. MoO3-x nanodots were synthesized by pulsed laser ablation (PLA) method in MoS2 nanosheets solutions, which may act directly as numerous fine targets. MoO3-x nanodots showed a uniform and monodispersed morphology, and the tiny dots were around 3-5 nm with a narrow size distribution. Due to the efficient charge transition between Mo5+/Mo6+ on the dots surface, MoO3-x nanodots exhibited excellent catalase and SOD mimic activities, which were adopted to alleviate Aß-mediated oxidative stress. Moreover, MoO3-x nanodots can efficiently inhibit Aß aggregation and destabilize the preformed fibrils, and eventually protect neuronal cells from apoptosis induced by Aß. Taken together, MoO3-x nanodots with multifunctional roles can act as a potential therapeutic strategy for treatment of amyloid induced neurotoxicity.


Assuntos
Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/toxicidade , Molibdênio/química , Molibdênio/farmacologia , Nanopartículas/química , Neurônios/efeitos dos fármacos , Neurônios/patologia , Óxidos/química , Óxidos/farmacologia , Apoptose/efeitos dos fármacos , Catalase/química , Sobrevivência Celular/efeitos dos fármacos , Humanos , Molibdênio/metabolismo , Neurônios/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Óxidos/metabolismo , Tamanho da Partícula , Agregados Proteicos/efeitos dos fármacos , Espécies Reativas de Oxigênio/análise , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/química , Propriedades de Superfície , Células Tumorais Cultivadas
4.
Inflamm Res ; 2018 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-30008029

RESUMO

AIM: The study aimed to investigate the effects of DNA repair proteins on cell apoptosis in human DPSCs during inflammation. METHODS: Lipopolysaccharide (LPS) was used to stimulate inflammation in dental pulp in vivo and in vitro. We identified the activation of DSB response and DNA repair proteins in inflamed pulp tissue and in LPS-treated human DPSCs. Then we transfected the cells with Ku70 (a key protein involved in NHEJ) siRNA and detected the expression changes of γ-H2A.X, DNA repair proteins and cell apoptosis. RESULTS: Immunohistochemical staining showed that at 4 and 6 days of pulpitis the expression of Ku70 and γ-H2A.X significantly increased. The levels of γ-H2A.X, Ku70, Xrcc4, and Rad51 increased considerably in the LPS-treated DPSCs. Furthermore, decreased expression of Ku70 could increase the number of γ-H2A.X foci, apoptotic cells and reduce cell viability in DPSCs. CONCLUSIONS: The results indicate that NHEJ pathway was the main mechanism involved in DNA damage response induced by repeated LPS stimulation in DPSCs. Meanwhile, the findings suggested that Ku70 serves importantly in the apoptosis of DPSCs in the inflammatory environment.

5.
Colloids Surf B Biointerfaces ; 162: 296-305, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29216517

RESUMO

Nanomaterials with visible light-driven photocatalytic activity have attracted much attention due to their excellent abilities in degradation of various organic pollutants as well as inactivating bacteria. Herein, graphene oxide (GO) enwrapped silver chloride/silver (AgCl/Ag) nanocomposites with high visible light absorption were designed and fabricated as efficient antibacterial agents. AgCl NPs were synthesized in the presence of GO first and Ag NPs were coated on AgCl surface by heat reduction to form GO-AgCl/Ag nanocomposites. The as prepared nanocomposites revealed improved stability, higher absorption properties in the visible light region. The enhanced antibacterial activity was observed by quantification of colony forming units (CFU) and morphological changes of bacteria. The antibacterial mechanism of GO-AgCl/Ag was also investigated by evaluating membrane permeability and ROS level. Moreover, GO-AgCl/Ag composites can eliminate bacterial biofilms more efficiently under visible light irradiation. Our results provide new insights into the design of new multifunctional systems for antibacterial applications.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Grafite/farmacologia , Nanopartículas Metálicas/química , Nanocompostos/química , Compostos de Prata/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Animais , Antibacterianos/química , Biofilmes/efeitos da radiação , Catálise , Sobrevivência Celular/efeitos dos fármacos , Grafite/química , Luz , Nanopartículas Metálicas/efeitos da radiação , Nanopartículas Metálicas/ultraestrutura , Camundongos , Células NIH 3T3 , Nanocompostos/efeitos da radiação , Nanocompostos/ultraestrutura , Óxidos , Processos Fotoquímicos , Compostos de Prata/química , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus aureus/efeitos da radiação
6.
J Endod ; 44(3): 414-431, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29273495

RESUMO

INTRODUCTION: The aim of this review was to evaluate whether the apical diameter of teeth with necrotic pulp affects the outcomes of regenerative endodontic treatment and to determine the minimal apical size needed to obtain proper pulp revascularization. METHODS: A literature search was performed from January 1, 2001, to November 25, 2016. Studies that satisfied the inclusion criteria were subjected to data extraction and analysis. RESULTS: In total, 14 studies with 85 patients were included. There were 10 case reports, 3 case series, and 1 prospective cohort study. The apical diameters of the teeth were divided into 3 groups: a narrow-sized group (group N), <0.5 mm (n = 10); a medium-sized group (group M), 0.5-1.0 mm (n = 25); and a wide-sized group (group W), >1.0 mm (n = 60). In group N, 1 tooth failed, 2 teeth completely healed, and 7 teeth incompletely healed. In group M, 2 teeth were excluded, and 1 tooth failed. In group W, 3 teeth were excluded, and 4 teeth failed. The clinical success rates were 90%, 95.65%, and 92.98% in groups N, M, and W, respectively. CONCLUSIONS: Within the limitations, the teeth with apical diameters <1.0 mm achieved clinical success after regenerative endodontic treatment. Meanwhile, the teeth with apical diameters of 0.5-1.0 mm attained the highest clinical success rate, which may be related to other potential factors, including patient age, pulp necrosis etiology, preoperative apical radiolucency, procedure details, follow-up period, and sample size.

7.
Oncotarget ; 8(42): 72182-72196, 2017 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-29069778

RESUMO

Diffuse large B-cell lymphoma (DLBCL) is one of the leading causes of cancer-related mortality, and responds badly to existing treatment. Thus, it is of urgent need to identify novel prognostic markers and therapeutic targets of DLBCL. Recent studies have shown that long non-coding RNAs (lncRNAs) play an important role in the development of cancer. By using the next generation HiSeq sequencing assay, we determined lncRNAs exhibiting differential expression between DLBCL patients and healthy controls. Then, RT-qPCR was performed for identification in clinical samples and cell materials, and lncRNA PANDA was verified to be down-regulated in DLBCL patients and have considerable diagnostic potential. In addition, decreased serum PANDA level was correlated to poorer clinical outcome and lower overall survival in DLBCL patients. Subsequently, we determined the experimental role of lncRNA PANDA in DLBCL progression. Luciferase reporter assay and chromatin immunoprecipitation assay suggested that lncRNA PANDA was induced by p53 and p53 interacts with the promoter region of PANDA. Cell functional assay further indicated that PANDA functioned as a tumor suppressor gene through the suppression of cell growth by a G0/G1 cell cycle arrest in DLBCL. More importantly, Cignal Signal Transduction Reporter Array and western blot assay showed that lncRNA PANDA inactivated the MAPK/ERK signaling pathway. In conclusion, our integrated approach demonstrates that PANDA in DLBCL confers a tumor suppressive function through inhibiting cell proliferation and silencing MAPK/ERK signaling pathway. Thus, PANDA may be a promising therapeutic target for patients with DLBCL.

8.
Analyst ; 142(13): 2500-2506, 2017 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-28589198

RESUMO

Due to low cost and high stability, the applications of inorganic nanomaterials as efficient alternatives to natural enzymes are drawing much attention. In this work, novel CuO/Pt nanocomposites with high peroxidase-like activity were designed and applied for the colorimetric detection of ascorbic acid (AA). The nanocomposites were prepared by decorating Pt NPs on the surface of CuO nanosheets, which displayed good uniformity and showed improved distribution and stability. The catalytic activity of the prepared CuO/Pt nanocomposites was tested against various chromogenic substrates in the presence of H2O2, which displayed efficient peroxidase-like activity and high catalytic stability against temperature. The catalytic mechanism of the CuO/Pt nanocomposites was investigated by hydroxyl radical detection. The peroxidase-like activity decreased significantly in the presence of AA. On the basis of the inhibition property, a colorimetric biosensor was constructed by using the CuO/Pt nanocomposites for the detection of AA. It showed a high selectivity against amino acids, carbohydrates and normal ions. Thus, this work provides new insights into the application of inorganic nanocomposite-based nanozymes in the biosensing field.

9.
ACS Appl Mater Interfaces ; 9(25): 21116-21123, 2017 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-28613069

RESUMO

The complex pathogenic mechanisms of Alzheimer's disease (AD) include the aggregation of ß-amyloid peptides (Aß) into oligomers or fibrils as well as Aß-mediated oxidative stress, which require comprehensive treatment. Therefore, the inhibition of Aß aggregation and free-radical scavenging are essential for the treatment of AD. Nanoparticles (NPs) have been found to influence Aß aggregation process in vitro. Herein, we report the inhibition effects of molybdenum disulfide (MoS2) NPs on Aß aggregation. Polyvinylpyrrolidone-functionalized MoS2 NPs were fabricated by a pulsed laser ablation method. We find that MoS2 NPs exhibit multifunctional effects on Aß peptides: inhibiting Aß aggregation, destabilizing Aß fibrils, alleviating Aß-induced oxidative stress, as well as Aß-mediated cell toxicity. Moreover, we show that MoS2 NPs can block the formation of the Ca2+ channel induced by Aß fibrils in the cell membrane for the first time. Thus, these observations suggest that MoS2 NPs have great potential for a multifunctional therapeutic agent against amyloid-related diseases.

10.
Nanoscale ; 9(18): 5927-5934, 2017 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-28436514

RESUMO

Single or few-layered MoS2 nanosheets, as a novel class of 2D nanomaterials, have received tremendous attention due to their fantastic physical and chemical properties. Here, we fabricated MoS2-PEG-CpG with a small and uniform size as a multifunctional platform for photothermal enhanced immunotherapy. MoS2 nanosheets were fabricated by chemical exfoliation and further probe sonication. To realize MoS2-based adjuvant delivery, MoS2 nanosheets were functionalized with cytosine-phosphate-guanine (CpG) and polyethylene glycol (PEG) to form MoS2-PEG-CpG nanoconjugates. As an efficient nanocarrier with excellent near infrared-light (NIR) absorbing performance, MoS2-PEG-CpG significantly promotes CpG intracellular accumulation and the effect can be further enhanced by photothermal treatment. In addition, the enhanced uptake can stimulate the production of proinflammatory cytokines and remarkably elevate the immune response level. Finally, we found that MoS2-PEG-CpG could reduce the proliferative activity of cancer cells when co-cultured with a macrophage-like cell upon NIR irradiation, implying a novel strategy for multifunctional therapeutics against cancers.


Assuntos
Ilhas de CpG , Imunoterapia , Molibdênio , Nanoconjugados , Neoplasias/tratamento farmacológico , Fototerapia , Animais , Linhagem Celular Tumoral , Camundongos , Camundongos Endogâmicos C57BL , Células RAW 264.7
11.
Environ Toxicol Pharmacol ; 51: 45-50, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28282589

RESUMO

This study investigated the correlation between differentially expressed proteins in amniotic fluid (AF) and cleft palate induced by all-trans retinoic acid (atRA), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice. Seven proteins were differentially expressed at embryonic day (E) 16.5 in atRA and control groups as revealed by label-based mouse antibody array. Enzyme-linked immunosorbent assay was further used to detect the expression levels of these proteins in AF from E13.5 to E16.5 in atRA, TCDD, and control groups. The cleft palate groups showed lower concentrations of receptor for advanced glycation end products (RAGE) and epiregulin at E16.5. RAGE immunostaining obviously decreased in palatal tissue sections obtained from E14.5 to E16.5 in the cleft palate groups as revealed by immunohistochemistry. These findings indicate that reduced levels of RAGE and epiregulin in AF are correlated to chemically induced cleft palate in mice.


Assuntos
Líquido Amniótico/metabolismo , Fissura Palatina/metabolismo , Epirregulina/metabolismo , Receptor para Produtos Finais de Glicação Avançada/metabolismo , Animais , Biomarcadores/metabolismo , Fissura Palatina/induzido quimicamente , Modelos Animais de Doenças , Feminino , Idade Gestacional , Camundongos Endogâmicos C57BL , Palato/efeitos dos fármacos , Palato/embriologia , Palato/metabolismo , Dibenzodioxinas Policloradas/toxicidade , Gravidez , Receptor para Produtos Finais de Glicação Avançada/genética , Tretinoína/toxicidade
12.
Nat Commun ; 8: 14364, 2017 02 24.
Artigo em Inglês | MEDLINE | ID: mdl-28232668

RESUMO

Non-syndromic cleft lip with palate (NSCLP) is the most serious sub-phenotype of non-syndromic orofacial clefts (NSOFC), which are the most common craniofacial birth defects in humans. Here we conduct a GWAS of NSCLP with multiple independent replications, totalling 7,404 NSOFC cases and 16,059 controls from several ethnicities, to identify new NSCLP risk loci, and explore the genetic heterogeneity between sub-phenotypes of NSOFC. We identify 41 SNPs within 26 loci that achieve genome-wide significance, 14 of which are novel (RAD54B, TMEM19, KRT18, WNT9B, GSC/DICER1, PTCH1, RPS26, OFCC1/TFAP2A, TAF1B, FGF10, MSX1, LINC00640, FGFR1 and SPRY1). These 26 loci collectively account for 10.94% of the heritability for NSCLP in Chinese population. We find evidence of genetic heterogeneity between the sub-phenotypes of NSOFC and among different populations. This study substantially increases the number of genetic susceptibility loci for NSCLP and provides important insights into the genetic aetiology of this common craniofacial malformation.


Assuntos
Fenda Labial/genética , Fissura Palatina/genética , Loci Gênicos/genética , Predisposição Genética para Doença , Adulto , Fatores Etários , Grupo com Ancestrais do Continente Asiático/etnologia , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , Fenda Labial/etnologia , Fissura Palatina/etnologia , Estudos de Coortes , Feminino , Estudo de Associação Genômica Ampla , Técnicas de Genotipagem , Humanos , Masculino , Polimorfismo de Nucleotídeo Único , Fatores Sexuais
13.
ACS Appl Mater Interfaces ; 8(37): 24404-14, 2016 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-27576084

RESUMO

Nucleic acid-based therapy has emerged as a revolutionary methodology for treatment of the diseases related to protein dysfunction; however, lack of systemically applicable synthetic delivery systems limits its current usage in local applications, particularly for DNA-based therapy with regard to the poor bioavailability in the systemic administrations. To overcome this obstacle, we compiled multiple chemistry-based strategies into the manufacture of the gene delivery formulations to pursue improved tolerability of DNA to the enzymatic degradation in the biological milieu and prolonged retention in the systemic circulation. Here, we constructed a distinctive multilayered functional architecture: plasmid DNA (pDNA) was electrostatically complexed with cationic poly(lysine) (polyplex) as the interior pDNA reservoir, which was further cross-linked by redox-responsive disulfide cross-linking to minimize the occurrence of polyplex disassembly through exchange reaction with the biological charged components. Still, the pDNA reservoir was spatially protected by a sequential thermoresponsive poly(N-isopropylacrylamide) palisade as the intermediate barrier and a biocompatible hydrophilic poly(ethylene glycol) (PEG) shell with the aim of preventing the accessibility of the biological species, particularly the nuclease degradation to the pDNA payload. Subsequent investigations validated the utilities of these strategies in accomplishing prolonged blood retention. In an attempt to apply this method for tumor therapy, ligand cyclic (Arg-Gly-Asp) peptide was attached at the distal end of PEG, validating prompted tumor-targeted delivery and gene expression of the loaded antiangiogenic gene at the targeted tumor cells and accordingly exerting antiangiogenesis of the tumors for abrogation of tumor growth. Together with its excellent safe profile, the proposed formulation suggests potential utility as a practical gene delivery system for treatment of intractable diseases.


Assuntos
Nanoestruturas , Linhagem Celular Tumoral , Técnicas de Transferência de Genes , Humanos , Micelas , Neoplasias , Plasmídeos , Polietilenoglicóis , Polímeros , Transfecção
14.
ACS Appl Mater Interfaces ; 8(4): 2511-6, 2016 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-26745637

RESUMO

Nanowires have attracted much attention due to their potential bioapplications, such as delivery of drugs or sensing devices. Here we report the development of a unique cell-capture and release platform based on nanowires. The combination of nanowires, surface-binding peptides, and cell-targeting aptamers leads to specific and efficient capture of cancer cells. Moreover, the binding processes are reversible, which is not only useful for downstream analysis but also for reusability of the substrate. Our work provides a new method in the design of the cell-capture and release platform, which may open up new opportunities of developing cell-separation and diagnosis systems based on cell-capture techniques.


Assuntos
Aptâmeros de Peptídeos/química , Nanofios/química , Adsorção , Gálio/química , Humanos , Células MCF-7 , Microscopia de Fluorescência
15.
Phys Chem Chem Phys ; 17(33): 21576-82, 2015 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-26220437

RESUMO

Today cancer is one of the most life-threatening diseases in the world. The conventional cancer therapies, including surgery, chemo- and radiation therapies, have some disadvantages, such as limited efficiency and significant side effects. It is necessary to develop new therapeutic treatments. Herein, we integrated the targeted photocatalytic and chemotherapy in a multifunctional drug-delivery platform. The aptamer-functionalized ZnO nanoparticles (NPs) were successfully synthesized. The anti-cancer drug was loaded in the aptamer-ZnO NP system. In vitro cell cytotoxicity experiments showed that combined therapy had a higher rate of death of cancer cells compared to that of single photocatalytic or chemotherapy. Furthermore, aptamer-functionalization could greatly increase the accumulation of nanoparticles within cancer cells and lead to better therapeutic effects. The results suggest that aptamer-functionalized semiconductor nanoparticles may have potential in the development of targeted photocatalytic and chemotherapy against cancer.


Assuntos
Antineoplásicos/química , Aptâmeros de Nucleotídeos/química , Portadores de Fármacos/química , Nanopartículas Metálicas/química , Óxido de Zinco/química , Antineoplásicos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/química , Doxorrubicina/toxicidade , Células HEK293 , Humanos , Luz , Células MCF-7 , Silanos/química
16.
Arch Oral Biol ; 60(3): 501-7, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25555252

RESUMO

OBJECTIVE: Glycogen synthase kinase-3ß (Gsk-3ß)/ß-catenin signaling regulates development of the secondary palate. It has been unclear about the effects of Gsk-3ß/ß-catenin signaling on palatal fusion and osteogenic differentiation in palatal shelves. DESIGN: In this study, palatal shelves from mouse embryonic day 13 (E13) were cultured in vitro with or without lithium chloride (LiCl). Palatal fusion was evaluated by haematoxylin-eosin staining. The expression of osteogenic markers in palatal shelves was measured by quantitative PCR, and immunohistochemical staining. Cell proliferation and apoptosis were examined by Ki-67 immunohistochemical and TUNEL staining, respectively. Gsk-3ß expression was evaluated by quantitative PCR and Western blotting. ß-catenin protein expression was evaluated by Western blotting. RESULTS: After the treatment with 10 mM LiCl, palatal shelves failed to fuse, and the mRNA and protein levels of osteogenic markers were reduced compared with controls. The number of Ki67-positive cell in the palatal osteoid was significantly higher in the LiCl group than in the controls. The apoptotic cells in the midline epithelial seam were reduced by LiCl. Gsk-3ß mRNA and protein expression levels decreased and ß-catenin protein expression levels increased by treatment of LiCl. CONCLUSION: Our findings show that LiCl-mediated GSK3ß inhibition prevents palatal fusion and osteogenic differentiation in palatal shelves by increased ß-catenin signaling. It indicated that overactivation of canonical Wnt signaling might impair the fusion of the secondary palate.


Assuntos
Fissura Palatina/induzido quimicamente , Cloreto de Lítio/toxicidade , Osteogênese/efeitos dos fármacos , Palato/efeitos dos fármacos , Palato/embriologia , Animais , Apoptose , Western Blotting , Proliferação de Células/efeitos dos fármacos , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Camundongos , Reação em Cadeia da Polimerase , RNA Mensageiro/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Proteínas Wnt/efeitos dos fármacos , beta Catenina/metabolismo
17.
Colloids Surf B Biointerfaces ; 123: 293-301, 2014 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-25264283

RESUMO

Semiconductors are important materials used for the development of high-performance biomedical devices. Gallium nitride (GaN) is a well-known III-nitride semiconductor with excellent optoelectronic properties as well as high chemical stability and biocompatibility. The formation of tight interfaces between GaN substrates and cells would be crucial for GaN-based devices used for probing and manipulating biological processes of cells. Here we report a strategy to greatly enhance cell adhesion and survival on nanotextured GaN surface which was treated by UV illumination and fibronectin (FN) adsorption. Cell studies showed that the UV/FN treatment greatly enhanced cell adhesion and growth on nanotextured GaN surfaces. These observations suggest new opportunities for novel nanotextured GaN-based biomedical devices.


Assuntos
Fibronectinas/química , Gálio/química , Gálio/farmacologia , Animais , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Humanos , Camundongos , Células NIH 3T3 , Nanoestruturas/química , Semicondutores , Raios Ultravioleta
18.
Small ; 10(21): 4386-94, 2014 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-25059878

RESUMO

A novel and convenient method to synthesize the nanocomposites combining graphene oxides (GO) with gold nanoparticles (AuNPs) is reported and their applications to modulate amyloid peptide aggregation are demonstrated. The nanocomposites produced by pulsed laser ablation (PLA) in water show good biocompatibility and solubility. The reduced aggregation of amyloid peptides by the nanocomposites is confirmed by Thioflavin T fluorescence and atomic force microscopy. The cell viability experiments reveals that the presence of the nanocomposites can significantly reduce the cytotoxicity of the amyloid peptides. Furthermore, the depolymerization of peptide fibrils and inhibition of their cellular cytotoxicity by GO/AuNPs is also observed. These observations suggest that the nanocomposites combining GO and AuNPs have a great potential for designing new therapeutic agents and are promising for future treatment of amyloid-related diseases.


Assuntos
Proteínas Amiloidogênicas/metabolismo , Ouro/química , Grafite/química , Terapia a Laser/métodos , Lasers , Nanocompostos/química , Agregação Patológica de Proteínas/prevenção & controle , Água/química , Proteínas Amiloidogênicas/química , Sobrevivência Celular , Células Cultivadas , Humanos , Terapia a Laser/instrumentação , Teste de Materiais , Óxidos/química , Peptídeos/química , Peptídeos/metabolismo , Agregação Patológica de Proteínas/metabolismo
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