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1.
Front Aging Neurosci ; 14: 860762, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35721018

RESUMO

Background: Knee osteoarthritis (KOA) is the leading cause of pain and stiffness, affecting older adults' physical function and quality of life. As a form of mind-body exercise, Tai Chi has been recommended as an exercise prescription for KOA patients. This study examined the effects and continuation of modified Tai Chi exercises on physical function and quality of life in elderly women with KOA. Methods: We conducted a single-blind, randomized controlled trial (RCT) on 40 older women with KOA. The participants were randomized to a 12 weeks Tai Chi or control group. The Tai Chi group attended a kind of modified Tai Chi training sessions three times per week; the control group attended wellness education sessions once a week. The primary outcome was the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Secondary outcomes were the Berg Balance Scale (BBS), Timed Up and Go (TUG), Short-Form 36 (SF-36), Pittsburgh Sleep Quality of Index (PSQI), Self-rating Anxiety Scale (SAS), and Self-rating Depression Scale (SDS). Results: After the 12-weeks the Tai Chi group showed significan improvement in the WOMAC pain (mean difference, -5.09 points, p = 0.001), WOMAC stiffness (mean difference, -3.60 points, p = 0.002), WOMAC physical function (mean difference, -11.21 points, p = 0.001) compared to the control group. In addition, the Tai Chi group had also significant improvement in the BBS (mean difference, 1.70 points, p = 0.008), TUG (mean difference, -0.52s, p = 0.001), SF-36PCS (mean difference, 7.60 points, p = 0.001), MCS (mean difference, 7.30 points, p = 0.001), PSQI (mean difference, -3.71 points, p = 0.001), SDS (mean difference, -5.37 points, p = 0.025) and SAS (mean difference, -5.06 points, p = 0.002). Conclusion: The modified Tai Chi exercises are an effective treatment for improved physical function and quality of life in elderly women with KOA. Clinical Trial Registration: The trial was registered in Chinese Clinical Trial Registry (ChiCTR2000040721), http://www.chictr.org.cn/edit.aspx?pid=65419&htm=4.

2.
J Hazard Mater ; 430: 128434, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35739655

RESUMO

CeO2/TiO2 catalyst is a promising material for realizing the integration of denitrification and mercury removal to reduce mercury emissions. Oxidation mechanism of Hg0 on CeO2/TiO2 (001) surface in the presence of HCl and O2 was studied by density functional theory (DFT). The results indicated that Hg0 was physically adsorbed on CeO2/TiO2 (001) surface. As an important intermediate, HgCl was adsorbed on the surface of CeO2/TiO2 (001) utilizing enhanced chemisorption, while the adsorption energy of HgCl2 was only -57.05 kJ/mol. In the absence of HCl, mercury oxidation followed the Mars-Maessen mechanism with a relatively high energy barrier, and the product (HgO) was difficult to desorb, which hindered the reaction process. When HCl existed, reactive chlorine (Cl*) would be produced by the dissociation of HCl, and the mercury oxidation would follow the Langmuir-Hinshelwood mechanism. The co-existence of HCl and O2 had no significant effect on the adsorption of Hg0, but reduced the reaction energy barrier and the final product (HgCl2) was more easily desorbed from the catalyst surface. In addition, two complete cyclic reaction pathways for catalytic oxidation of Hg0 on CeO2/TiO2 (001) surface were constructed to clarify the detailed reaction process.

3.
BMC Biol ; 20(1): 139, 2022 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-35698132

RESUMO

BACKGROUND: Peach (Prunus persica) is an economically important stone fruit crop in Rosaceae and widely cultivated in temperate and subtropical regions, emerging as an excellent material to study the interaction between plant and environment. During its genus, there are four wild species of peach, all living in harsh environments. For example, one of the wild species, P. mira, originates from the Qinghai-Tibet Plateau (QTP) and exhibits strong cold/ultraviolet ray environmental adaptations. Although remarkable progresses in the gene discovery of fruit quality-related traits in peach using previous assembled genome were obtained, genomic basis of the response of these wild species to different geographical environments remains unclear. RESULTS: To uncover key genes regulating adaptability in different species and analyze the role of genetic variations in resistance formation, we performed de novo genome assembling of four wild relatives of peach (P. persica), P. mira, P. davidiana, P. kansuensis, and P. ferganensis and resequenced 175 peach varieties. The phylogenetic tree showed that the divergence time of P. mira and other wild relatives of peach was 11.5 million years ago, which was consistent with the drastic crustal movement of QTP. Abundant genetic variations were identified in four wild species when compared to P. persica, and the results showed that plant-pathogen interaction pathways were enriched in genes containing small insertions and deletions and copy number variations in all four wild relatives of peach. Then, the data were used to identify new genes and variations regulating resistance. For example, presence/absence variations which result from a hybridization event that occurred between P. mira and P. dulcis enhanced the resistance of their putative hybrid, P. davidiana. Using bulked segregant analysis, we located the nematode resistance locus of P. kansuensis in chromosome 2. Within the mapping region, a deletion in the promoter of one NBS-LRR gene was found to involve the resistance by regulating gene expression. Furthermore, combined with RNA-seq and selective sweeps analysis, we proposed that a deletion in the promoter of one CBF gene was essential for high-altitude adaptation of P. mira through increasing its resistance to low temperature. CONCLUSIONS: In general, the reference genomes assembled in the study facilitate our understanding of resistance mechanism of perennial fruit crops, and provide valuable resources for future breeding and improvement.


Assuntos
Prunus persica , Cromossomos , Variações do Número de Cópias de DNA , Evolução Molecular , Genoma de Planta , Filogenia , Melhoramento Vegetal , Prunus persica/genética
4.
Arch Virol ; 2022 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-35716268

RESUMO

A lytic Pseudomonas aeruginosa phage, vB_PaeP_Lx18 (Lx18), was isolated from the sewage of a dairy farm. Biological characterization revealed that Lx18 was stable from 40 °C to 60 °C and over a wide range of pH values from 4 to 10. It was able to lyse 63.6% (21/33) of the P. aeruginosa strains tested and was able to reduce and disperse biofilms, with a biofilm reduction rate of 76.8%. Whole-genome sequencing showed that Lx18 is a dsDNA virus with a genome of 42,735 bp and G+C content of 62.16%. The genome contains 54 open reading frames (ORFs), 28 of which have known functions, including DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis. No virulence or tRNA genes were identified. Phylogenetic analysis showed that phage Lx18 belongs to the genus Phikmvvirus. The lysozyme of Lx18, Lys18, was cloned and expressed. The combined action of Lys18 and ethylenediaminetetraacetic acid (EDTA) had antibacterial activity against Pseudomonas aeruginosa. The study of phage Lx18 and its lysozyme will provide basic information for further research on the treatment of Pseudomonas aeruginosa infections.

5.
Opt Lett ; 47(11): 2919-2922, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35648964

RESUMO

A smart vector-inspired optical vision guiding (VIOVG) method for autonomous underwater vehicle (AUV) docking and formation is proposed. Unlike traditional optical guiding methods based on LED arrays, the method is inspired by a vector, and uses four laser diodes to form a wing-light pattern to realize smart optical guiding for AUVs. Due to the light scattering effect from water, the four laser diodes can emit four bright and slim laser beams in water, which can be captured by underwater cameras. The intersections of the laser beams form wing-lights as markers for AUV distance and pose estimation. The wing-light pattern is easily tuned and extended by changing the propagation direction and the power of the laser beams beyond the limitation of the physical size of the AUV or docking station. The simulations and experiments show that the proposed method can achieve high precision positioning. A relative distance error of 3.35% is achieved in a positioning experiment at ∼10 m. It has great potential for AUV docking and formation, especially for small AUVs.

6.
Front Oncol ; 12: 749119, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35651807

RESUMO

Purpose: To systematically investigate the characterization of tumor microenvironment (TME) in clear cell renal cell carcinoma (ccRCC), we performed a comprehensive analysis incorporating genomic alterations, cellular interactions, infiltrating immune cells, and risk signature. Patients and Methods: Multi-omics data including RNA-seq, single-nucleotide variant (SNV) data, copy number variation (CNV) data, miRNA, and corresponding prognostic data were obtained from The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) database. The CIBERSORT algorithm was utilized to identify prognostic TME subclusters, and TMEscore was further quantified. Moreover, the mutational landscape of TCGA-KIRC was explored. Lastly, TIDE resource was applied to assess the significance of TMEscore in predicting immunotherapeutic benefits. Results: We analyzed the TME infiltration patterns from 621 ccRCC patients and identified 5 specific TME subclusters associated with clinical outcomes. Then, we found that TMEcluster5 was significantly related to favorable prognosis and enriched memory B-cell infiltration. Accordingly, we depicted the clustering landscape of TMEclusters, TMEscore levels, tumor mutation burden (TMB), tumor grades, purity, and ploidy in all patients. Lastly, TIDE was used to assess the efficiency of immune checkpoint blockers (ICBs) and found that the TMEscore has superior predictive significance to TMB, making it an essential independent prognostic biomarker and drug indicator for clinical use. Conclusions: Our study depicted the clustering landscape of TMEclusters, TMEscore levels, TMB, tumor grades, purity, and ploidy in total ccRCC patients. The TMEscore was proved to have promising significance for predicting prognosis and ICB responses, in accordance with the goal of developing rationally individualized therapeutic interventions.

7.
Microbiol Spectr ; : e0049622, 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35579466

RESUMO

To investigate the contribution of a tet(A) variant to tigecycline resistance in Enterobacter hormaechei and the recombination events that occurred during transmission of this variant. MICs were determined by broth microdilution. E. hormaechei G17 was characterized by PCR, transfer assay, S1-PFGE, Southern blot hybridization, and WGS analysis. A tet(A) variant conferring resistance to tigecycline was present in E. hormaechei G17. This strain harbored two resistance plasmids (pG17-1, 264,084 bp and pG17-2, 68,610 bp) and its E. coli transformant Tm-G17TGC one resistance plasmid (pTm-G17, 93,013 bp). The comparative analysis of pG17-1, pG17-2, and pTm-G17 showed that a tet(A) variant-carrying multiresistance gene cluster (~23 kb) originating from pG17-1 had integrated into pG17-2, forming the novel plasmid pTm-G17. In a first step, this multiresistance gene cluster was excised from pG17-1 by recombination of homologous sequences, including △TnAs1 at both termini, thereby generating an unconventional circularizable structure (UCS). In a second step, this UCS integrated into pG17-2 via recombination between homologous sequences, including IS26 present on both, the UCS and pG17-2, thereby giving rise to the new plasmid pTm-G17. In summary, a tet(A) variant conferring resistance to tigecycline was reported in E. hormaechei. Transfer of a tet(A) variant-carrying multiresistance gene cluster between plasmids occurred in a two-step recombination process, in which homologous sequences, including either △TnAs1 or IS26, were involved. IMPORTANCE Tigecycline is an important last-resort broad spectrum antimicrobial agent. This study describes the two-step recombination processes resulting in the transfer of the tet(A) variant gene between different plasmids in E. hormaechei, which depicts the role of recombination processes in the generation of UCSs and new plasmids, both carrying a tet(A) variant conferring resistance to tigecycline. Such processes enhance the dissemination of resistance genes, which is of particular relevance for resistance genes, such as the tet(A) variant. The presence and transmission of a tet(A) variant in E. hormaechei will compromise the efficacy of tigecycline treatment for E. hormaechei associated infection.

8.
BMC Infect Dis ; 22(1): 471, 2022 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-35578187

RESUMO

BACKGROUND: Vaccination has been proven to be an effective approach against the coronavirus disease 2019 (COVID-19) pandemic. This study aimed to determine the acceptance rate and factors influencing acceptance of COVID-19 vaccination among people living with HIV (PLWH) in Guangxi, China. METHODS: A cross-sectional survey was carried out in five cities in Guangxi, China from May 7 to June 1, 2021. Questionnaires on the acceptance of COVID-19 vaccination and the related factors were conducted among PLWH recruited by simple random sampling. Univariate and multivariate logistic regression analyses were performed to identify factors associated with acceptance of COVID-19 vaccination. RESULTS: Of all valid respondents (n = 903), 72.9% (n = 658) were willing to receive COVID-19 vaccination. Fear of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was the main reason for being willing to receive vaccination (76.0%), while the main reasons for not willing were the concerns about vaccine safety (54.7%) and the vaccination's effect on antiretroviral therapy (ART) (50.6%). The most important factors influencing acceptance were the perception that vaccination is unsafe for HIV-infected people (aOR = 0.082, 95% CI = 0.024-0.282) and the poor efficacy in preventing SARS-CoV-2 infection in HIV-infected people (aOR = 0.093, 95% CI = 0.030-0.287). Other factors associated with acceptance included Zhuang ethnicity (aOR = 1.653, 95% CI = 1.109-2.465), highest education level of middle school, high school or above (aOR = 1.747, 95% CI = 1.170-2.608; aOR = 2.492, 95% CI = 1.326-4.682), and the vaccination having little effect on ART efficacy (aOR = 2.889, 95% CI = 1.378-6.059). CONCLUSIONS: Acceptance rate of the COVID-19 vaccination is relatively low among PLWH compared to the general population in China, although some patients refused vaccination due to concerns about vaccine safety and vaccination affecting ART efficacy. More research is needed to investigate the impact of the COVID-19 vaccines on ART efficacy and the effectiveness in preventing SARS-CoV-2 infection among PLWH.


Assuntos
COVID-19 , Infecções por HIV , COVID-19/epidemiologia , COVID-19/prevenção & controle , Vacinas contra COVID-19 , China/epidemiologia , Estudos Transversais , Humanos , SARS-CoV-2 , Inquéritos e Questionários , Vacinação
9.
10.
RSC Adv ; 12(13): 8154-8159, 2022 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-35424761

RESUMO

SiBZrOC quinary ceramics were obtained through the modification of a SiOC precursor with B(OH)3 and Zr(OnPr)4. The results showed that both B and Zr atoms were involved in the SiOC network through Si-O-B and Si-O-Zr bonds, respectively. The combined effects of B and Zr on the chemical structure and the thermal stability of the SiBZrOC system were investigated in detail. The sp3-C/Si ratio of SiBZrOC ceramics was between the values for SiZrOC and SiBOC. The presence of B promotes the crystallization of t-ZrO2, which precipitated at 1000 °C and transformed to m-ZrO2 at 1400 °C. At 1600 °C, ZrO2 reacted with the matrix and formed ZrSiO4, which consumed SiO2 and thus inhibited the carbothermal reaction. The very small I(D)/I(G) ratio of 0.13 in the Raman spectra indicated the high graphitization of free carbon in SiBZrOC ceramics, which was observed by TEM with 10-20 graphene layers. The SiBZrOC ceramics showed excellent thermal stability in argon at 1600 °C for 5 h with a mass loss of 6%. Both the formation of ZrSiO4 and the highly graphitized free carbon play important roles in inhibiting the carbothermal reaction and thus improving the thermal stability of SiBZrOC ceramics.

11.
RSC Adv ; 12(3): 1375-1383, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-35425177

RESUMO

To improve the performance of lithium-sulfur (Li-S) batteries, herein, based on the idea of designing a material that can adsorb polysulfides and improve the reaction kinetics, a Co,N-co-doped graphene composite (Co-N-G) was prepared. According to the characterization of Co-N-G, there was a homogeneous and dispersed distribution of N and Co active sites embedded in the Co-N-G sample. The 2D sheet-like microstructure and Co, N with a strong binding energy provided significant physical and chemical adsorption functions, which are conducive to the bonding S and suppression of LiPSs. Moreover, the dispersed Co and N as catalysts promoted the reaction kinetics in Li-S batteries via the reutilization of LiPSs and reduced the electrochemical resistance. Thus, the discharge specific capacity in the first cycle for the Co-N-G/S battery reached 1255.7 mA h g-1 at 0.2C. After 100 cycles, it could still reach 803.0 mA h g-1, with a retention rate of about 64%. This phenomenon proves that this type of Co-N-G composite with Co and N catalysts plays an effective role in improving the performance of batteries and can be further studied in Li-S batteries.

12.
J Diabetes Res ; 2022: 8895950, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35372585

RESUMO

Objective: The mechanism of Panax notoginseng in treating myocardial fibrosis (MF) was investigated using network pharmacology. Methods: Effective ingredients and potential targets of Panax notoginseng were screened in relevant databases to construct a compound-target network. Targets of MF were then screened to select common targets and construct a protein-protein interaction network. This was followed by Gene Ontology and pathway enrichment analyses. Molecular docking then verified the results of network analysis. Results: A total of 14 effective ingredients and 119 potential targets for MF were predicted. Quercetin, beta-sitosterol, and gossypetin were speculated to be the main active ingredients. The mechanism of action may be related to AGE-RAGE, proteoglycans, and IL-17 signaling pathways. Five key targets (IL6, ALB, AKT1, TNF, and VEGFA) may be involved in the treatment of MF using Panax notoginseng. Conclusions: This study embodies the complex network relationship of multicomponents, multitargets, and multipathways of Panax notoginseng in treating MF and provides a novel method for further research on this herb's mechanism.


Assuntos
Medicamentos de Ervas Chinesas , Panax notoginseng , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Fibrose , Simulação de Acoplamento Molecular
13.
Microbiol Spectr ; : e0221221, 2022 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-35384693

RESUMO

Ebola virus, a member of the Filoviridae family, utilizes the attachment factors on host cells to support its entry and cause severe tissue damage. TIM-1 has been identified as a predominant attachment factor via interaction with phosphatidylserine (PS) localized on the viral envelope and glycoprotein (GP). In this study, we give the first demonstration that TIM-1 enhances the cellular entry of three species of Ebola virus, as well as those harboring GP mutations (A82V, T544I, and A82V T544I). Furthermore, two TIM-1 variants (i.e., TIM-1-359aa and TIM-1-364aa) had comparable effects on promoting Zaire Ebola virus (EBOV) attachment, internalization, and infection. Importantly, recombinant TIM-1 ectodomain (ECD) protein could decrease the infectivity of Ebola virus and display synergistic inhibitory effects with ADI-15946, a monoclonal antibody with broad neutralizing activity to Ebola virus. Of note, EBOV strains harboring GP mutations (K510E and D552N), which were refractory to antibody treatment, were still sensitive to TIM-1 protein-mediated impairment of infectivity, indicating that TIM-1 protein may represent an alternative therapeutic regimen when antibody evasion occurs. IMPORTANCE The viral genome has acquired numerous mutations with the potential to increase transmission during the 2013-to-2016 outbreak of Ebola virus. EBOV strains harboring GP mutations (A82V, T544I, and A82V T544I), which have been identified to increase viral infectivity in humans, have attracted our attention. Herein, we give the first report that polymorphic TIM-1 enhances the infectivity of three species of Ebola virus, as well as those harboring GP mutations (A82V, T544I, and A82V T544I). We show that recombinant TIM-1 ECD protein could decrease the infectivity of Ebola virus with or without a point mutation and displays synergistic inhibitory effects with ADI-15946. Furthermore, TIM-1 protein potently blocked cell entry of antibody-evading Ebola virus species. These findings highlight the role of TIM-1 in Ebola virus infection and indicate that TIM-1 protein represents a potential therapeutic avenue for Ebola virus and its mutated species.

14.
Front Vet Sci ; 9: 871826, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35419450

RESUMO

Chicken infectious anemia (CIA) is an immunosuppressive disease caused by the chicken infectious anemia virus (CIAV) resulting in heavy economic losses once an outbreak is established. This study conducted a systematic analysis of the epidemiology and pathology of CIA in Henan province, China. A total of 437 clinical tissue samples and 120 poultry disease-related live attenuated vaccines were collected during 2017-2020; of which 45 were positive for CIAV nucleic acid, with a positive rate of 8.08%. Our results showed that genome sequence similarity among a total of 12 CIAV isolates was high, and ranged from 97.1 to 99.3%, and their similarity to the vaccine strains Cux-1 and Del-Ros ranged from 97.8 to 98.6%. However, There were mutations in the locus of the major capsid proteins VP1, VP2, and VP3 among all isolates. The subsequent sequence analysis indicated that the isolates of HN-4 and HN-8 showed genetic recombination and follow up animal experiments revealed that HN-4 might be a pathogenic strain. Our results reveal that both field infection and non-CIAV vaccines contamination promote the epidemiology of CIAV in China and some dominant epidemic viruses have undergone recombination and evolution. This study provides important information to help with the prevention and control of CIAV in the poultry industry.

15.
Cartilage ; 13(2): 19476035221093060, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35438034

RESUMO

OBJECTIVE: The present study explored whether low-intensity pulsed ultrasound (LIPUS) enhances the therapeutic efficacy of mesenchymal stem cells (MSCs) in osteoarthritis (OA) cartilage repair by regulating autophagy-mediated exosome release. DESIGN: MSCs were isolated from the rat bone marrow and treated with rapamycin, 3-methyladenine, or LIPUS. The mechanism of the LIPUS-stimulated exosome release by MSCs was analyzed by inhibiting autophagy. In addition, the MSCs were co-cultured with OA chondrocytes and stimulated by LIPUS, with or without exosome release inhibitor intervention. The exosome release was detected through transmission electron microscopy (TEM), nanoparticle tracking analysis, and biomarker expression analysis. Autophagy was analyzed through TEM, autophagy-related gene expression analysis, and immunofluorescence analysis in vitro. Furthermore, a rat knee OA model was constructed and treated with MSCs, GW4869, and LIPUS. The cartilage repair was assessed through histopathological analysis and extracellular matrix protein expression analysis. RESULTS: The in vitro results indicated that LIPUS promoted MSC exosome release by activating autophagy. The in vivo results demonstrated that LIPUS significantly enhanced the positive effects of MSCs on OA cartilage. These effects were significantly blocked by GW4869, an inhibitor of exosome release. CONCLUSIONS: LIPUS can enhance the therapeutic efficacy of MSCs in OA cartilage repair, and the underlying mechanism is related to the increase in autophagy-mediated exosome release.


Assuntos
Células-Tronco Mesenquimais , Osteoartrite do Joelho , Animais , Autofagia , Medula Óssea , Osteoartrite do Joelho/terapia , Ratos , Ondas Ultrassônicas
16.
Nat Commun ; 13(1): 2188, 2022 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-35449139

RESUMO

Computational meta-optics brings a twist on the accelerating hardware with the benefits of ultrafast speed, ultra-low power consumption, and parallel information processing in versatile applications. Recent advent of metasurfaces have enabled the full manipulation of electromagnetic waves within subwavelength scales, promising the multifunctional, high-throughput, compact and flat optical processors. In this trend, metasurfaces with nonlocality or multi-layer structures are proposed to perform analog optical computations based on Green's function or Fourier transform, intrinsically constrained by limited operations or large footprints/volume. Here, we showcase a Fourier-based metaprocessor to impart customized highly flexible transfer functions for analog computing upon our single-layer Huygens' metasurface. Basic mathematical operations, including differentiation and cross-correlation, are performed by directly modulating complex wavefronts in spatial Fourier domain, facilitating edge detection and pattern recognition of various image processing. Our work substantiates an ultracompact and powerful kernel processor, which could find important applications for optical analog computing and image processing.


Assuntos
Processamento de Imagem Assistida por Computador , Óptica e Fotônica , Computadores , Análise de Fourier , Processamento de Imagem Assistida por Computador/métodos
17.
Int Immunopharmacol ; 108: 108678, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35364431

RESUMO

Aryl hydrocarbon receptor (Ahr) is thought to be a crucial factor that regulates immune responses, which may be involved in the pathogenesis of autoimmune inflammation including rheumatoid arthritis (RA). The results of our group in recent years have shown that Paeoniflorin-6'-O-benzene sulfonate (code: CP-25), a novel ester derivative of paeoniflorin, has a good effect on improving RA animal models. However, whether the anti-arthritis effect of CP-25 is related to Ahr remains unclear. Here, we showed that CP-25 treatment ameliorated adjuvant-induced arthritis (AA), a rat model of RA, by inhibiting Ahr-related activities in fibroblasts like synoviocytes (FLS). AA rats were treated with CP-25 or paroxetine from days 17 to 33 after immunization. We showed that CP-25 alleviated arthritis symptoms and the pathological changes. Treatment with CP-25 decreased the expression of Ahr in the synovium of AA rats. CP-25 inhibited the expression of Ahr and the G protein-coupled receptor kinase 2 (GRK2) as well as the co-expression of GRK2 with Ahr in FLS of AA rats. Furthermore, CP-25 down-regulated the production of Kyn in FLS of AA rats. These results suggested that CP-25 may inhibit the expression and activation of Ahr. Besides, treatment with CP-25 reduced the proliferation and migration of MH7A caused by Ahr activation. In addition, we also demonstrated that CP-25 down-regulated the total and nuclear expression of Ahr and the expression of GRK2 in Kyn-treated MH7A. Moreover, the co-expression and co-localization of Ahr and GRK2in Kyn-treated MH7A were also repressed by CP-25. The data presented here demonstrated that CP-25 suppressed FLS dysfunction in rats with AA, which were associated with reduced Ahr activation and the interaction between Ahr and GRK2.


Assuntos
Artrite Experimental , Artrite Reumatoide , Sinoviócitos , Animais , Artrite Experimental/patologia , Artrite Reumatoide/metabolismo , Proliferação de Células , Células Cultivadas , Fibroblastos , Glucosídeos , Monoterpenos , Ratos , Receptores de Hidrocarboneto Arílico/metabolismo , Membrana Sinovial/patologia
18.
Sci Total Environ ; 834: 155354, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35460773

RESUMO

Deoxynivalenol (DON) and fumonisin B1 (FB1), as a group of highly toxic secondary metabolites, have become a potential source of water environmental pollutants. To minimize two mycotoxins exposure to consumers, a dual lateral flow immunoassay (LFIA) integrated with the smartphone was reported for simultaneous and quantitative detection of DON and FB1 in the water environment. The significantly improved sensitivity was contributed to a smartphone-based device with the ability to image and analyze results. Under optimized conditions, the detection limits of DON and FB1 were calculated to be 3.46 and 2.65 ng/mL, which were approximately 25 and 10 folds lower than those of the visual detection of the LFIA. This method showed good specificity and a good dynamic linear detection for DON and FB1. The recoveries of DON and FB1 were evaluated by the spiked lake water, river water, and pond water, ranging from 92.47% to 106.2% with the relative standard deviation under 9.13%. Moreover, the results of the developed LFIA showed a high correlation with enzyme-linked immunosorbent assay (ELISA) results, with a correlation coefficient of 0.999 for DON and 0.996 for FB1, respectively. To sum up, the developed LFIA provides a promising platform for sensitive, simultaneous, quantitative, and on-site detection of DON and FB1 in the water environment.


Assuntos
Fumonisinas , Micotoxinas , Fumonisinas/análise , Fumonisinas/toxicidade , Imunoensaio , Micotoxinas/análise , Smartphone , Tricotecenos , Água
19.
Pain Physician ; 25(2): E309-E317, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35322986

RESUMO

BACKGROUND: Lumbar disc herniation (LDH) is the most common cause of sciatica. Percutaneous endoscopic discectomy (PELD) is indicated when conservative treatments fail, which has been proved effective. During conventional PELD, ruptured discs and loose fragments inside discs are removed as much as possible to guarantee a lower reherniation rate, but it inevitably would lead to deterioration of disc degeneration and loss of disc height after PELD. Ensuring sufficient decompression while alleviating the post-operation disc degeneration process is still a clinical problem. OBJECTIVE: To evaluate the imaging and clinical outcomes of bi-needle PELD with intradiscal irrigation technique for the treatment of lumbar disc herniation (LDH). STUDY DESIGN: Multicenter retrospective cohort study. SETTING: Shanghai Changzheng Hospital, Naval Medical University, Shanghai, China. METHODS: A total of 48 patients who underwent bi-needle PELD (B-PELD) or conventional-PELD (C-PELD) for LDH in our 2 spine centers were included in this study. There were 26 cases in the C-PELD group (male 12 cases, female 14 cases) with an average age of 34.6 ± 6.8 years. And there were 22 patients in the B-PELD group (male 10 cases, female 12 cases) with an average age of 35.1 ± 6.4 years. The difference in postoperative disc degeneration (Pfirrmann grades, disc-vertebra height ratios [D-V H ratios]), visual analog scale (VAS) of low back pain, and reoperation rates were compared between the 2 groups. RESULTS: There was no significant difference in gender, age, disease duration, and surgical level between the 2 groups (P > 0.05). The postoperative VAS of back pain was 2.31 ± 0.53 for the C-PELD group and 0.63 ± 0.74 for the B-PELD group; the difference was significant (P = 0.013). The difference between the preoperative and postoperative D-V H ratios in the C-PELD group was significant (P < 0.0001), while it was not significant in the B-PELD group (P = 0.6708). The difference between the loss of D-V H ratios after surgery was significant between the 2 groups (P = 0.0003). The loss of D-V H ratios was higher in the C-PELD group. The difference between the preoperative and postoperative Pfirrmann grades in the B-PELD group was not significant (P = 0.7261); however, it was significant in the C-PELD group (P = 0.0012). The reoperation rate in the C-PELD group was 7.7%, and the reoperation rate in the B-PELD group was 4.5%; the difference was not significant (P = 1). LIMITATIONS: This study employed a retrospective design, and its inherent selection bias and limited statistical power should be considered. CONCLUSIONS: Bi-needle technique with saline irrigation maneuver showed a significant advantage of restoration of disc height and amelioration of disc degeneration compared to conventional PELD surgery.


Assuntos
Discotomia Percutânea , Degeneração do Disco Intervertebral , Deslocamento do Disco Intervertebral , Adulto , China , Discotomia Percutânea/métodos , Endoscopia/métodos , Feminino , Humanos , Degeneração do Disco Intervertebral/cirurgia , Deslocamento do Disco Intervertebral/cirurgia , Vértebras Lombares/cirurgia , Masculino , Estudos Retrospectivos , Resultado do Tratamento
20.
Phys Chem Chem Phys ; 24(12): 7195-7202, 2022 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-35262534

RESUMO

Antimony sulfide (Sb2S3) and selenide (Sb2Se3) have emerged as promising earth-abundant alternatives among thin-film photovoltaic compounds. A distinguishing feature of these materials is their anisotropic crystal structures, which are composed of quasi-one-dimensional (1D) [Sb4X6]n ribbons. The interaction between ribbons has been reported to be van der Waals (vdW) in nature and Sb2X3 are thus commonly classified in the literature as 1D semiconductors. However, based on first-principles calculations, here we show that inter-ribbon interactions are present in Sb2X3 beyond the vdW regime. The origin of the anisotropic structures is related to the stereochemical activity of the Sb 5s lone pair according to electronic structure analysis. The impacts of structural anisotropy on the electronic, dielectric and optical properties relevant to solar cells are further examined, including the presence of higher dimensional Fermi surfaces for charge carrier transport. Our study provides guidelines for optimising the performance of Sb2X3-based photovoltaics via device structuring based on the underlying crystal anisotropy.

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