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1.
Medicine (Baltimore) ; 100(5): e24007, 2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-33592857

RESUMO

RATIONALE: This study aimed to investigate the genetic mutation characteristics of congenital idiopathic hypogonadotropic hypogonadism (IHH) through the clinical features and genetic analysis of 2 patients with IHH in 1 pedigree. PATIENT CONCERNS: A 23-year-old girl presented with primary amenorrhea, sparse pubic hair, lack of breast development, and delayed sexual development. DIAGNOSES: Combined with the clinical characteristics, auxiliary examinations, and molecular genetic analysis, the patient was diagnosed as IHH. INTERVENTIONS: Whole exome and Sanger sequencing were performed to validate the mutation in family members. OUTCOMES: A novel homozygous missense mutation c.521A > G (p.Q174R) in the GNRHR gene was identified in the 2 affected sisters. Familial segregation showed that the homozygous variant was inherited from their parents respectively and the eldest sister was the carrier without correlative symptom. LESSONS: We reported a novel GNRHR mutation in a pedigree with congenital idiopathic hypogonadotropic hypogonadism. Glutamine at amino acid position 174 was highly conserved among various species. The molecular structure of GNRHR protein showed that p.Q174R mutation brought in a new stable hydrogen bond between position 174 and 215, may impede conformational mobility of the TMD4 and TMD5. It suggests that the missense mutation c.521A > G related to congenital idiopathic hypogonadotropic hypogonadism was probably a causative factor for both sisters. Through high-throughput sequencing and experimental verification, we had basically determined the patient's pathogenic mutation and inheritance, which could better guide doctors for treatment.


Assuntos
Hipogonadismo , Receptores LHRH/genética , Feminino , Predisposição Genética para Doença , Testes Genéticos/métodos , Homozigoto , Humanos , Hipogonadismo/congênito , Hipogonadismo/genética , Hipogonadismo/fisiopatologia , Mutação de Sentido Incorreto , Linhagem , Irmãos , Adulto Jovem
3.
Sci Prog ; 104(1): 36850420987043, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33449868

RESUMO

In order to solve the problem of freezing damage treatment and anti-freezing design for railway tunnels in operation in the seasonal frozen area, this paper proposes a polyurethane-corrugated steel plate insulation lining, and studies its thermal insulation effect, relying on a freezing damage treatment project of an existing passenger-dedicated tunnel for on-site application. In this paper, the insulation lining is applied to the actual project and the insulation effect is verified by the field monitoring. The temperature test section was set in the anti-freezing design section. The polyurethane-corrugated steel plate insulation lining proposed in this paper is mainly composed of corrugated steel plate, polyurethane insulation layer and waterproof layer. The polyurethane-corrugated steel insulation lining is applied by using self-made rail flat battery car, door-type scaffolding, arch slide and other construction equipments. The on-site temperature test shows that the insulation effect is obvious. The polyurethane-corrugated steel plate insulation lining has the advantages of fast construction speed, good thermal insulation effect, preventing concrete from peeling off blocks, easy removal and replacement. According to the field temperature test, under the natural temperature outside the tunnel from -18°C to -22°C in winter of 2018-2020, the temperature of the interface between surrounding rock and lining of the tunnel monitoring sections are all above 0°C so that the freezing damage is eliminated from the root. Research results can provide a theoretical basis for the anti-freezing projects of tunnels in seasonal frozen area.

4.
Brain Behav ; 10(10): e01796, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32815287

RESUMO

INTRODUCTION: Autism spectrum disorder (ASD) is a complex and prevalent neurodevelopmental disorder characterized by deficits in social communication and social interaction as well as repetitive behaviors. Alterations in function connectivity are widely recognized in recent electroencephalogram (EEG) studies. However, most studies have not reached consistent conclusions, which could be due to the developmental nature and the heterogeneity of ASD. METHODS: Here, EEG coherence analysis was used in a cohort of children with ASD (n = 13) and matched typically developing controls (TD, n = 15) to examine the functional connectivity characteristics in long-distance and short-distance electrode pairs. Subsequently, we explore the association between the connectivity strength of coherence and symptom severity in children with ASD. RESULTS: Compared with TD group, individuals with ASD showed increased coherence in short-distance electrode pairs in the right temporal-parietal region (delta, alpha, beta bands), left temporal-parietal region (all frequency bands), occipital region (theta, alpha, beta bands), right central-parietal region (delta, alpha, beta bands), and the prefrontal region (only beta band). In the long-distance coherence analysis, the ASD group showed increased coherence in bilateral frontal region, temporal region, parietal region, and frontal-occipital region in alpha and beta bands. The strength of such connections was associated with symptom severity. DISCUSSION: Our study indicates that abnormal connectivity patterns in neuroelectrophysiology may be of critical importance to acknowledge the underlying brain mechanism.

5.
Cyberpsychol Behav Soc Netw ; 22(2): 127-131, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30779616

RESUMO

We examined and analyzed binocular imbalance in a group of randomly selected normal subjects with corrected visual acuity of 0.8 or more in both eyes without ocular diseases, by using augmented virtual reality system. This study will quantify the correlation between binocular imbalance in these normal subjects and various spatial frequencies and temporal frequencies in the integrated binocular stimulation mode. A retrospective study was carried out and data from 97 patients (51 males and 46 females) who were diagnosed with no eye diseases, and with corrected binocular visual acuity of 0.8 or more, were collected. Their binocular visual function, Hirschberg test refraction status, and binocular imbalance were assessed using the quantified visual perception stimulation mode in an augmented virtual reality platform. When normal subjects (with corrected binocular vision of 0.8 or more without ocular diseases) were assessed using the integrated binocular stimulation mode with different temporal and spatial frequencies, a gradual reduction of binocular imbalance could be observed when spatial frequencies were increased. When contrast balance was adjusted, binocular imbalance was more prevalent in the horizontal state compared with the vertical state. This was seen when patients were stimulated by different temporal frequencies in the virtual reality testing mode. 17.53% and 29.90% of participants reached complete binocular fusion under low temporal frequency stimulation and high temporal frequency stimulation, respectively, while binocular imbalance was found in 65.9% and 62.89% of participants who were stimulated, respectively, by low temporal frequency and high temporal frequency. In addition, 16.5% and 7.21% experienced severe binocular imbalance under low temporal and high temporal frequency stimulation, respectively. The integrated binocular stimulation mode under low temporal frequency was more sensitive in detecting binocular imbalance. The status of binocular balance in normal subjects was not the same when stimulated by the augmented virtual reality platform, as many showed varying degrees of binocular imbalance. The results from our clinical study demonstrate that integrated binocular stimulation with different spatial and temporal frequencies enables a more sensitive detection of binocular imbalance, making it a powerful new tool for the diagnosis and analysis of clinical binocular abnormalities. In addition, since more than half of the normal subjects (without eye diseases who has corrected vision of 0.8 or more) exhibited binocular imbalance in our study, we believe that this brief binocular imbalance is likely a transient physiological phenomenon, which is not pathological to visual acuity and function, and should only be seen as pathological when binocular imbalance is prolonged, causing functional deficits such as reading impairment.


Assuntos
Realidade Virtual , Visão Binocular/fisiologia , Percepção Visual/fisiologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estimulação Luminosa/métodos , Estudos Retrospectivos , Adulto Jovem
6.
BMC Psychiatry ; 18(1): 283, 2018 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-30180836

RESUMO

BACKGROUD: Autism spectrum disorders (ASD) are a complex group of neurodevelopmental disorders with a genetic basis. The role of long-chain polyunsaturated fatty acids (LC-PUFAs) and the occurrence of autism has been the focus of many recent studies. The present study investigates whether genetic variants of the fatty acid desaturase (FADS) 1/2 and elongation of very long-chain fatty acids protein (ELOVL) 2 genes, which are involved in LC-PUFA metabolism, are associated with ASD risk. METHODS: A cohort of 243 ASD patients and 243 unrelated healthy controls were enrolled in this case control study. Sixteen tag single nucleotide polymorphisms from the FADS1-2 and ELOVL2 genes were genotyped using the Sequenom Mass Array. RESULTS: There were significant differences in allelic distribution of FADS2 rs526126 (OR = 0.55, 95% CI = 0.42-0.72, pFDR < 0.05) between autistic children and controls. FADS2 rs526126 and ELOVL2 rs10498676 were associated with decreased ASD risk in recessive model (OR = 0.07, 95% CI = 0.02-0.22, pFDR < 0.01; OR = 0.56, 95% CI = 0.35-0.89, pFDR = 0.042), while ELOVL2 rs17606561, rs3756963, and rs9468304 were associated with increased ASD risk in overdominant model (OR = 1.63, 95% CI = 1.12-2.36, pFDR = 0.036; OR = 1.64, 95% CI = 1.14-2.37, pFDR = 0.039; OR = 1.75, 95% CI = 1.22-2.50, pFDR = 0.017). The A/A genotype of rs10498676 was correlated with a decline in the Autism Diagnostic Interview-Revised communication (verbal and nonverbal) domain. CONCLUSIONS: These findings provide evidence of an association between FADS2 and ELOVL2 polymorphisms and ASD susceptibility in Chinese children.


Assuntos
Acetiltransferases/genética , Grupo com Ancestrais do Continente Asiático/genética , Transtorno do Espectro Autista/genética , Ácidos Graxos Dessaturases/genética , Polimorfismo de Nucleotídeo Único/genética , Transtorno do Espectro Autista/diagnóstico , Transtorno do Espectro Autista/epidemiologia , Estudos de Casos e Controles , Criança , Pré-Escolar , China/epidemiologia , Estudos de Coortes , Elongases de Ácidos Graxos , Feminino , Predisposição Genética para Doença/epidemiologia , Predisposição Genética para Doença/genética , Humanos , Masculino
7.
Metab Brain Dis ; 32(4): 1033-1042, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28299627

RESUMO

Autism spectrum disorder (ASD) is a neurodevelopment disorder with abnormalities of social interaction, communication and repetitive behaviors. The higher prevalence of ASD in men implies a potential relationship between sex hormones and ASD etiology. The ESR2 gene encodes estrogen receptor beta (ESR2) and plays an important role during brain development. A relationship between ESR2 and ASD has been suggested by studies on single nucleotide polymorphisms and mRNA and protein expression levels in ASD patients. Here, we explored the possible epigenetic regulation of the ESR2 gene in autism. We collected genomic DNA from the peripheral blood of Chinese Han males with autism and age-matched normal males and measured DNA methylation of CpG islands in the ESR2 gene, which consisted of 41 CpG sites among the proximal promoter region and an untranslated exon, by bisulfite sequencing. We also investigated a relationship between DNA methylation and phenotypic features of autism, as assessed by the Children Autism Rating Scale. We found little overall difference in the DNA methylation of the ESR2 5'-flanking region in individuals with autism compared with normal individuals. However, detailed analyses revealed that eight specific CpG sites were hypermethylated in autistic individuals and that four specific CpG sites were positively associated with the severity of autistic symptoms. Our study indicates that the epigenetic dysregulation of ESR2 may govern the development of autism.


Assuntos
Transtorno Autístico/genética , Metilação de DNA , Receptor beta de Estrogênio/genética , Grupo com Ancestrais do Continente Asiático/genética , Pré-Escolar , China , Ilhas de CpG/genética , Humanos , Masculino , Regiões Promotoras Genéticas
8.
Life Sci ; 173: 43-54, 2017 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-28161158

RESUMO

AIMS: To investigate the effect of FTY720 on the valproic acid (VPA) rat model of autism. MAIN METHODS: As an animal model of autism, we used intraperitoneal injection of VPA on embryonic day 12.5 in Wistar rats. The pups were given FTY720 orally at doses of 0.25, 0.5 and 1mg/kg daily from postnatal day 15 to 35. Social behavior, spatial learning and memory were assessed at the end of FTY720 treatment. The histological change, oxidative stress, neuroinflammatory responses, and apoptosis-related proteins in the hippocampus were evaluated. KEY FINDINGS: FTY720 (1mg/kg) administration to VPA-exposed rats (1) improved social behavior, spatial learning and memory impairment; (2) resulted in a reduction in neuronal loss and apoptosis of pyramidal cells in hippocampal CA1 regions; (3) inhibited activation of microglial cells, in turn lowering the level of pro-inflammatory cytokines interleukin-1ß (IL-1ß) and IL-6 in the hippocampus; (4) changed Malondialdehyde (MDA) levels, Glutathione (GSH) levels, superoxide dismutase (SOD) activity and Glutathione Peroxidase (GSH-Px) activity in the hippocampus; (6) inhibited the elevated Bax and caspase-3 protein levels and enhanced the relative expression level of Bcl-2 in the hippocampus; and (7) increased phospho-Ca2+/calmodulin-dependent protein kinase II (p-CaMKII), phospho-cAMP-response element binding protein (p-CREB) and Brain Derived Neurotrophic Factor (BDNF) protein expression in the hippocampus. SIGNIFICANCE: FTY720 rescues social deficit, spatial learning and memory impairment in VPA-exposed rats. FTY720 exerts both a direct protection for neurons and an indirect modulation of inflammation-mediated neuron loss as a possible mechanism of neuroprotection.


Assuntos
Apoptose/efeitos dos fármacos , Transtorno Autístico , Cloridrato de Fingolimode/efeitos adversos , Aprendizagem/efeitos dos fármacos , Transtornos da Memória , Células Piramidais/metabolismo , Comportamento Social , Animais , Proteínas Reguladoras de Apoptose/biossíntese , Transtorno Autístico/induzido quimicamente , Transtorno Autístico/metabolismo , Transtorno Autístico/patologia , Fator Neurotrófico Derivado do Encéfalo/biossíntese , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/biossíntese , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/biossíntese , Modelos Animais de Doenças , Feminino , Cloridrato de Fingolimode/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Inflamação/induzido quimicamente , Inflamação/metabolismo , Inflamação/patologia , Interleucina-1beta/biossíntese , Interleucina-6/biossíntese , Masculino , Malondialdeído/metabolismo , Transtornos da Memória/induzido quimicamente , Transtornos da Memória/metabolismo , Transtornos da Memória/patologia , Estresse Oxidativo/efeitos dos fármacos , Oxirredutases/biossíntese , Células Piramidais/patologia , Ratos , Ratos Wistar
9.
Neuro Endocrinol Lett ; 37(6): 439-444, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28315628

RESUMO

OBJECTIVES: Autism has a significant sex difference. This implies that the sex hormones might have effect on autism. Estrogens play an important role in early nervous system development and sex differentiation through estrogen receptors in brain. Thus, we tested the hypothesis that estrogen receptor alpha (ESR1) gene affects the pathogenesis of autism and related symptoms. METHODS: Genotypes of rs11155819 and rs2234693 were determined in boys with autism and normal boys from Chinese Han population. A case-control study was performed to explore the association between polymorphisms in ESR1 gene and autism susceptibility. Assessment tool was used to evaluate the neuropsychological developmental level of autistic children. Finally, we analyzed the association of these single nucleotide polymorphisms (SNPs) with specific symptoms. RESULTS: The results showed no significant differences between cases and controls in the distribution of genotypes and allele frequencies of the two SNPs. However, rs11155819 TT genotype showed a lower neuropsychological development level among autistic children, especially in the aspects of fine motor and adaptation ability (p=0.028; p=0.042). CONCLUSION: Polymorphisms of ESR1 are relevant to autism symptoms in Chinese Han children.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Transtorno Autístico/genética , Receptor alfa de Estrogênio/genética , Estudos de Associação Genética , Polimorfismo de Nucleotídeo Único/genética , Estudos de Casos e Controles , Criança , Pré-Escolar , Frequência do Gene/genética , Humanos , Masculino
10.
Int J Dev Neurosci ; 49: 67-78, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26639559

RESUMO

Prenatal exposure to valproic acid (VPA) in rat offspring is capable of inducing experimental autism with neurobehavioral aberrations. This study investigated the effect of docosahexaenoic acid (DHA) on hippocampal cell death, learning and memory alteration in an experimental rat autism model. We found that DHA supplementation (75, 150 or 300 mg/kg/day, 21 days) rescued the VPA (600 mg/kg) induced DHA reduction in plasma and hippocampus in a dose-dependent manner, increased the levels of hippocampal p-CaMKII and p-CREB without affecting total protein level, and altered BDNF-AKT-Bcl-2 signaling pathway, as well as inhibited the activity of caspase-3. DHA also influenced the content of malondialdehyde (MDA) and the activities of antioxidant enzymes in the VPA-treated offspring. Consistent with the previous results, we also observed that 300 mg/kg DHA supplementation markedly increased the cell survival, decreased the cell apoptosis, and increased mature neuronal cell in the hippocampus in VPA-treated offspring. Utilizing the Morris water maze test, we found that DHA prevented cognitive impairment in offspring of VPA-treated rats. The data suggested that DHA may play a neuroprotective role in hippocampal neuronal cell and ameliorates dysfunctions in learning and memory in this rat autism model. Thus, DHA could be used as treatment intervention for mitigating behavioral dysfunctions in autism spectrum disorder (ASD).


Assuntos
Transtorno Autístico/induzido quimicamente , Ácidos Docosa-Hexaenoicos/uso terapêutico , Hipocampo/patologia , Deficiências da Aprendizagem , Transtornos da Memória , Fármacos Neuroprotetores/uso terapêutico , Animais , Anticonvulsivantes/toxicidade , Transtorno Autístico/complicações , Caspase 3/metabolismo , Morte Celular/efeitos dos fármacos , Modelos Animais de Doenças , Ácidos Docosa-Hexaenoicos/metabolismo , Feminino , Glutationa Peroxidase/metabolismo , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Deficiências da Aprendizagem/tratamento farmacológico , Deficiências da Aprendizagem/etiologia , Deficiências da Aprendizagem/patologia , Masculino , Malondialdeído/metabolismo , Aprendizagem em Labirinto/efeitos dos fármacos , Transtornos da Memória/tratamento farmacológico , Transtornos da Memória/etiologia , Transtornos da Memória/patologia , Fármacos Neuroprotetores/metabolismo , Fosfopiruvato Hidratase/metabolismo , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismo , Ácido Valproico/toxicidade
11.
Int J Dev Neurosci ; 44: 14-21, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25916973

RESUMO

To investigate whether the decreased level of serum polyunsaturated fatty acids (PUFAs) in patients with autism is associated with the expression of related liver metabolic enzymes, we selected rats that were exposed to valproic acid (VPA) on embryonic day 12.5 (E12.5) as a model of autism. We observed the serum levels of PUFAs and the expression of related liver metabolic enzymes, including Δ5-desaturase, Δ6-desaturase and elongase (Elovl2), in VPA-exposed and control rats on postnatal day 35 (PND35) and conducted sex dimorphic analysis. We found that the levels of serum PUFAs and related liver metabolic enzymes in the VPA rats were significantly reduced, in association with autism-like behavioral changes, the abnormal expression of apoptosis-related proteins and hippocampal neuronal injury, compared to the control rats and showed sex difference in VPA group. This finding indicated that rats exposed to VPA at the embryonic stage may exhibit reduced synthesis of serum PUFAs due to the down-regulation of liver metabolic enzymes, thereby inducing nervous system injury and behavioral changes, which is affected by sex in the meantime.


Assuntos
Acetiltransferases/metabolismo , Anticonvulsivantes/toxicidade , Transtorno Autístico/sangue , Transtorno Autístico/induzido quimicamente , Ácidos Graxos Dessaturases/metabolismo , Ácidos Graxos Insaturados/sangue , Ácido Valproico/toxicidade , Acetiltransferases/genética , Animais , Caspase 3/metabolismo , Modelos Animais de Doenças , Comportamento Exploratório/efeitos dos fármacos , Comportamento Exploratório/fisiologia , Ácidos Graxos Dessaturases/genética , Elongases de Ácidos Graxos , Feminino , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Aprendizagem em Labirinto/fisiologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal/sangue , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/fisiopatologia , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Wistar , Fatores Sexuais , Proteína X Associada a bcl-2/metabolismo
12.
J Zhejiang Univ Sci B ; 15(3): 264-71, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24599690

RESUMO

OBJECTIVE: A study in a Caucasian population has identified two single-nucleotide polymorphisms (SNPs) in ZNF533, one in DOCK4, and two in IMMP2L, which were all significantly associated with autism. They are located in AUTS1 and AUTS5, which have been identified as autism susceptibility loci in several genome-wide screens. The present study aimed to investigate whether ZNF533, DOCK4, and IMMP2L genes are also associated with autism in a northeastern Chinese Han population. METHODS: We performed a similar association study using families with three individuals (one autistic child and two unaffected parents). A family-based transmission disequilibrium test (TDT) was used to analyze the results. RESULTS: There were significant associations between autism and the two SNPs of ZNF533 gene (rs11885327: χ(2)=4.5200, P=0.0335; rs1964081: χ(2)=4.2610, P=0.0390) and the SNP of DOCK4 gene (rs2217262: χ(2)=5.3430, P=0.0208). CONCLUSIONS: Our data suggest that ZNF533 and DOCK4 genes are linked to a predisposition to autism in the northeastern Chinese Han population.


Assuntos
Transtorno Autístico/genética , Endopeptidases/genética , Proteínas Ativadoras de GTPase/genética , Polimorfismo de Nucleotídeo Único/genética , Proteínas Repressoras/genética , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Criança , Pré-Escolar , China , DNA/química , DNA/genética , Família , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Proteínas Nucleares , Reação em Cadeia da Polimerase
13.
Dalton Trans ; 42(41): 14726-32, 2013 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-23887557

RESUMO

Front-side illuminated solar cells with CdS quantum dots (QDs) incorporated with free-standing through-hole TiO2 nanotube arrays (TNAs) were developed. The solar cells, based on TNAs with different lengths that were sensitized by successive ionic layer adsorption and reaction method (SILAR) with various cycles, have been tested. The morphology and crystalline phase of the TiO2 nanotubes were studied by field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). The crystallized free-standing through-hole TNAs were easily transferred to the fluorine-doped tin oxide glass to form a photoanode by slightly modifying the anodization procedure. The SILAR technique enables us to control the loading amount and particle size of CdS QDs by altering deposition cycles. The cells with TNAs ca. 20 µm long (obtained by anodization for 4 h) and 5 SILAR cycles show a photovoltaic conversion efficiency as high as 1.187% under simulated sunlight (AM 1.5, 100 mW cm(-2)).


Assuntos
Compostos de Cádmio/química , Fontes de Energia Elétrica , Nanotubos/química , Pontos Quânticos , Energia Solar , Sulfetos/química , Titânio/química , Tamanho da Partícula , Porosidade , Propriedades de Superfície
14.
J Nanosci Nanotechnol ; 13(2): 1461-6, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23646661

RESUMO

CdS quantum dots (QDs)-sensitized TiO2 film with three-dimensionally (3D) ordered macropores was synthesized via a two-step method on ITO glass substrate. 3D-ordered macroporous TiO2 film was firstly fabricated on an ITO glass via layer-by-layer deposition and hydrolysis of tetrabutyl titanate using 3D-ordered latex film as organic template, followed by calcination at 450 degrees C for 2 h to remove the template. Then, the CdS QDs were deposited on the 3D-ordered macroporous TiO2 film by successive ionic layer adsorption and reaction technique. The as-prepared CdS-sensitized TiO2 film was characterized with X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy, diffusive reflectance UV-visible absorption spectra, and photoelectrochemical measurements. Its photocatalytic activity was evaluated by the photocatalytic degradation of crystal violet aqueous solution at ambient temperature. It was revealed in our results that the CdS QDs-sensitized 3D-ordered macroporous TiO2 film exhibits enhanced photocatalytic activity for the photodegradation of crystal violet than that of the CdS-free 3D-ordered macroporous TiO2 film and that of CdS QD-sensitized TiO2 film without 3D-ordered macropores under the irradiation of visible light due to the co-existence of 3D-ordered interconnected macropores and the sensitization of CdS QDs.

15.
PLoS One ; 6(11): e27428, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22110649

RESUMO

BACKGROUND: Autism is a common, severe and highly heritable neurodevelopmental disorder in children, affecting up to 100 children per 10,000. The MET gene has been regarded as a promising candidate gene for this disorder because it is located within a replicated linkage interval, is involved in pathways affecting the development of the cerebral cortex and cerebellum in ways relevant to autism patients, and has shown significant association signals in previous studies. PRINCIPAL FINDINGS: Here, we present new ASD patient and control samples from Heilongjiang, China and use them in a case-control and family-based replication study of two MET variants. One SNP, rs38845, was successfully replicated in a case-control association study, but failed to replicate in a family-based study, possibly due to small sample size. The other SNP, rs1858830, failed to replicate in both case-control and family-based studies. CONCLUSIONS: This is the first attempt to replicate associations in Chinese autism samples, and our result provides evidence that MET variants may be relevant to autism susceptibility in the Chinese Han population.


Assuntos
Grupo com Ancestrais do Continente Asiático/etnologia , Grupo com Ancestrais do Continente Asiático/genética , Transtornos Globais do Desenvolvimento Infantil/genética , Grupos Étnicos/genética , Polimorfismo de Nucleotídeo Único/genética , Proteínas Proto-Oncogênicas c-met/genética , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Predisposição Genética para Doença/genética , Humanos , Masculino , Adulto Jovem
16.
J Hazard Mater ; 169(1-3): 1112-8, 2009 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-19500906

RESUMO

V-doped TiO(2)nanoparticles were synthesized at low temperature and characterized by X-ray diffraction (XRD), Raman spectroscopy (RS), transmission electron microscopy (TEM), Brunauer-Emmet-Teller (BET), X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, and photoluminescence (PL) spectroscopy, respectively. It is found the nanoparticle shape changed from needle, to short stick and then to cubic with the increase of doped V concentration, which was also accompanied by the improvement of crystallinity. The specific surface area (S(BET)) decreased with increasing V content. It is confirmed that V ions can be doped in TiO(2) by substituting Ti(4+) ions, which suppressed the CB --> VB and surface recombination of photoinduced electrons and holes, and a relation was found between the PL spectra and the UV photocatalytic activity. There was an optimum V content for the V-doped TiO(2) to present the best UV-light induced photoactivity, but they were visible-inactive. At last, the effect of the doping V as trapping centers on photocatalysis was investigated in detail, and used to explain the difference between the photocatalysis under the illumination of UV light and visible light.


Assuntos
Nanopartículas/química , Processos Fotoquímicos , Titânio/química , Vanádio/química , Catálise , Temperatura Baixa , Estrutura Molecular , Raios Ultravioleta
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