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1.
Chem Commun (Camb) ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031183

RESUMO

A two-photon excited "Ping-Pong" type energy transfer process is for the first time disclosed in a pyrene-modified Ir(iii) cyclometalated complex. The energy transfer, from the singlet excited state of the 4-(pyren-1-yl)-tpy (tpy-py) unit to the Ir(iii) moiety and then back again to the triplet excited state of the tpy-py unit, enhances both the two-photon absorption cross sections and singlet oxygen quantum yield of the complex, and dramatically boosts its two-photon photodynamic activity both in vitro and in 3D multicellular spheroids.

3.
J Hazard Mater ; 386: 121960, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31893555

RESUMO

Both heavy metals and microplastic pollutants are ubiquitous in the aquatic environment. The uptake of lead(II) ions from aqueous solutions onto aged nylon microplastics was investigated as a function of pH, contact time, temperature, supporting electrolyte concentration and fulvic acid concentration in batch studies. The effect of surface properties on the adsorption behavior of lead(II) was investigated with scanning electron microscope equipped with the energy dispersive X-ray spectroscope (SEM-EDAX), Fourier transform-infrared (FTIR) spectroscopy, thermal gravimetric analysis (TGA), X-ray diffraction (XRD) and differential scanning calorimetric (DSC). The adsorption kinetics conformed to the pseudo-second order equation, Elovich equation and intraparticle diffusion model well. The experimental data of the adsorption process was fitted to the Langmuir and Freundlich adsorption isotherms and the parameters were estimated. The lead(II) uptake on aged nylon microplastics was spontaneous and endothermic in nature. The lead(II) adsorption was significantly dependent on the sodium chloride concentrations, initial solution pH and fulvic acid concentrations. Results of this study highlight the importance of surface carboxyl function group of aged nylon microplastics in controlling lead(II) adsorption.

4.
Am Heart J ; 221: 84-94, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31954328

RESUMO

BACKGROUND: High-sensitivity cardiac troponin (hs-cTn) assays enhance detection of lower circulating troponin concentrations, but the impact on outcomes in clinical practice is unclear. Our objective was to compare outcomes of chest pain patients discharged from emergency departments (EDs) using hs-cTn and conventional troponin (cTn) assays. METHODS: We conducted an observational study of chest pain patients aged 40-105 years who presented to an ED from April 1, 2013, to March 31, 2017, and were discharged home. We compared 30-day and 1-year outcomes of EDs that used hs-cTn versus cTn assays. The primary outcome was a composite of all-cause death, myocardial infarction or unstable angina. Comparisons were conducted with (1) no adjustment; (2) adjustment for demographic, socioeconomic, and hospital characteristics; and (3) full clinical adjustment. RESULTS: Among the 394,910 patients, 62,138 (15.7%) were evaluated at hs-cTn EDs and 332,772 (84.3%) were evaluated at cTn EDs. Patients discharged from hs-cTn EDs were less likely to have diabetes, hypertension, or prior heart disease. At 30 days, the unadjusted primary outcome rate was lower in hs-cTn EDs (0.9% vs 1.0%, P < .001). The 30-day hazard ratios for the primary outcome were 0.84 (95% CI 0.77-0.92) for no adjustment and 0.98 (95% CI 0.88-1.08) for full adjustment. Over 1 year, patients discharged from hs-cTn EDs had significantly fewer primary outcomes (3.7% vs 4.1%, P < .001) and lower hazard ratio (0.93; 95% CI 0.89-0.98) even after full adjustment. CONCLUSIONS: Hs-cTn testing was associated with a significantly lower adjusted hazard of myocardial infarction, angina, and all-cause hospitalization at 1 year but not 30 days.

5.
Am J Sports Med ; 48(1): 117-127, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31765235

RESUMO

BACKGROUND: It has been speculated that the patellar J sign may have a negative effect on the clinical outcomes of patients with recurrent patellar dislocation (RPD). PURPOSE: To (1) evaluate clinical outcomes, postoperative patellar stability, and patellar maltracking correction in patients with RPD treated with derotational distal femoral osteotomy (DDFO) and combined procedures and (2) investigate the influence of J sign severity on the clinical outcomes. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: Between January 2015 and December 2016, a total of 78 patients (81 knees) with RPD, a positive J sign, and an excessive femoral anteversion angle (FAA; ≥30°) were surgically treated with DDFO and combined procedures. J sign severity was graded according to a previously described classification system (grades 1-3). Routine radiography and computed tomography were performed on every patient to evaluate the patellar height, trochlear dysplasia, genu valgum, tibial tuberosity-trochlear groove distance, patellar lateral tilt angle, and patella-trochlear groove distance. The patellar lateral shift distance during stress radiography was measured preoperatively and postoperatively to quantify medial patellofemoral ligament (MPFL) graft laxity under anesthesia, and "MPFL residual graft laxity" was defined as the patellar ridge surpassing the apex of the lateral femoral trochlea. Patients were evaluated using the Kujala, International Knee Documentation Committee (IKDC), and Lysholm scores preoperatively and postoperatively. Patients were allocated into 3 subgroups in terms of the severity of the J sign: low-grade group 1 (grade 1; n = 19), low-grade group 2 (grade 2; n = 16), and high-grade group (grade 3; n = 12). Subgroup analyses were performed to investigate the influence of a high-grade J sign on the clinical outcomes. RESULTS: Among the 78 patients (81 knees), 47 patients (47 knees) met the inclusion criteria. The mean follow-up time was 26.1 ± 1.7 months. The mean preoperative and postoperative FAAs were 36.2°± 5.3° and 10.0°± 2.1°, respectively, with a mean correction angle of 26.2°± 5.9°. At the final follow-up, all patient-reported outcomes improved significantly, and subgroup analyses showed that the high-grade group had significantly lower Kujala scores (75.6 vs 85.3 for low-grade group 1 [P < .001] and 83.4 for low-grade group 2 [P = .001]), Lysholm scores (77.6 vs 84.6 for low-grade group 1 [P = .003]), and IKDC scores (78.6 vs 87.3 for low-grade group 1 [P = .001] and 84.3 for low-grade group 2 [P = .033]) than the low-grade groups. The total rate of MPFL residual graft laxity was 8.5% (4/47), and the prevalence of the postoperative residual J sign was 38.3% (18/47). Subgroup analyses showed significant differences between the high-grade group and the 2 low-grade groups with regard to the MPFL residual graft laxity rate (33.3% vs 0.0% for low-grade group 1 [P = .016] and 0.0% for low-grade group 2 [P = .024]), residual J sign rate (91.7% vs 15.8% for low-grade group 1 [P < .001] and 25.0% for low-grade group 2 [P < .001]), and patellar lateral shift distance (14.2 vs 8.1 mm for low-grade group 1 [P = .002] and 8.7 mm for low-grade group 2 [P = .007]). CONCLUSION: In a group of patients treated for RPD with a positive preoperative J sign and increased FAA (≥30°), patients with a preoperative high-grade J sign had inferior clinical outcomes, more MPFL residual graft laxity, and greater residual patellar maltracking.

6.
Food Chem ; 310: 125947, 2020 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-31841939

RESUMO

An ultra performance liquid chromatography-Triple time of flight mass spectrometry (UPLC-Triple TOF-MS/MS) method were established to characterize the lipid profiles in four shellfish species. More than 600 lipid molecular species belonging to 14 classes were detected. Phospholipids (PLs) were predominant in Chlamys farreri (54.9%) and glycerolipids (GLs) were dominant in Ostrea gigas (51.6%). PLs that contained polyunsaturated fatty acids (PUFAs) such as PC (16:0/20:5), PC (16:0/22:6) and PE (18:0/22:6) were the main molecular species. Especially, the percentage of sphingolipids (SLs) in four shellfishes is considerable (18.8-38.6%), the characterization of their special long-chain base (LCB) structure (mainly d19:3) and N-acyl group (mainly 16:0) was realized. Several SL subclasses with low abundance in four shellfish species, such as ceramide 2-aminoethylphosphonate (CAEP) and deoxy-ceramide (DeoxyCer), were also detected. These active lipids identified by this method have potential value in revealing the nutritional value of shellfishes and serving as biomarkers for distinguishing different shellfishes.

7.
Ecotoxicol Environ Saf ; 188: 109858, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31706236

RESUMO

Cultivar-dependent cadmium (Cd) accumulation was principal in developing Cd-pollution safe cultivars (PSCs). Proteins related to different Cd accumulations of the low-Cd-accumulating (SJ19) and high-Cd-accumulating (CX4) cultivars were investigated by iTRAQ analysis. Higher Cd bioaccumulation factors and translocation factor in CX4 than in SJ19 were consistent with the cultivar-dependent Cd accumulations. The Cd uptake was promoted in CX4 due to its higher expression of Cd-binding proteins and the lower expression of Cd-efflux proteins in roots. What's more, significantly elevated thiol groups (PC2 and PC3) in CX4 under Cd stress might contribute to the high Cd accumulation in roots and the root-to-shoot translocation of Cd-PC complex. Up-regulated proteins involved in cellulose biosynthesis and pectin de-esterification in SJ19 enhanced the Cd sequestration of root cell walls, which was considered as the predominant strategy for reducing Cd accumulation in shoots. The present study provided novel insights in the cultivar-dependent Cd accumulation in shoots of B. parachinensis.


Assuntos
Brassica/metabolismo , Cádmio/metabolismo , Proteínas de Plantas/metabolismo , Poluentes do Solo/metabolismo , Transporte Biológico , Brassica/genética , Celulose/metabolismo , Pectinas/metabolismo , Raízes de Plantas/metabolismo , Brotos de Planta/metabolismo , Proteômica , Reagentes de Sulfidrila/metabolismo
8.
Aging (Albany NY) ; 11(24): 11803-11813, 2019 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-31866584

RESUMO

Increasing studies have demonstrated that long noncoding RNAs (lncRNAs) play vital roles in tumor development and progression. However, the relationship between osteosarcoma and HIF1AAS2 remains unknown. The expression of HIF1AAS2 and miR-129-5p was detected in osteosarcoma cell lines and samples via qRT-PCR. Cell Counting Kit-8 (CCK-8) and invasion assays were performed to determine cell proliferation and invasion ability, and a dual luciferase reporter assay was performed to determine the interaction between HIF1AAS2 and miR-129-5p. We showed that the expression of HIF1A-AS2 was upregulated in the osteosarcoma samples compared with the expression in noncancerous samples. Moreover, patients with high HIF1A-AS2 expression had a shorter overall survival. Ectopic expression of HIF1A-AS2 enhanced osteosarcoma cell proliferation, cell cycle progression and invasion. We found that overexpression of miR-129-5p decreased the luciferase activity of wild-type (WT) HIF1A-AS2 but not mutant HIF1A-AS2. Ectopic expression of HIF1A-AS2 suppressed miR-129-5p expression in MG-63 cells. We demonstrated that miR-129-5p was downregulated in osteosarcoma and was negatively associated with HIF1A-AS2 expression. Furthermore, ectopic expression of miR-129-5p suppressed osteosarcoma cell proliferation, cell cycle progression and invasion. In addition, overexpression of HIF1A-AS2 promoted cell proliferation, cell cycle progression and invasion of osteosarcoma cells through the modulation of miR-129-5p. These results indicated that HIF1A-AS2 might be a potential therapeutic target for osteosarcoma.

9.
Sensors (Basel) ; 19(23)2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31766679

RESUMO

High-resolution range profile (HRRP) has attracted intensive attention from radar community because it is easy to acquire and analyze. However, most of the conventional algorithms require the prior information of targets, and they cannot process a large number of samples in real time. In this paper, a novel HRRP recognition method is proposed to classify unlabeled samples automatically where the number of categories is unknown. Firstly, with the preprocessing of HRRPs, we adopt principal component analysis (PCA) for dimensionality reduction of data. Afterwards, t-distributed stochastic neighbor embedding (t-SNE) with Barnes-Hut approximation is conducted for the visualization of high-dimensional data. It proves to reduce the dimensionality, which has significantly improved the computation speed. Finally, it is exhibited that the recognition performance with density-based clustering is superior to conventional algorithms under the condition of large azimuth angle ranges and low signal-to-noise ratio (SNR).

10.
Chem Commun (Camb) ; 55(93): 14081-14084, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31696872

RESUMO

Cholesterol (CHOL) is an indispensable component of liposomes. Incorporation of 7-dehydrocholesterol (7-DHC) instead of CHOL can efficiently enhance the anticancer activity of photosensitizer-encapsulated liposomes upon irradiation, yielding an IC50 value about half of that of CHOL-based controls. The photo-oxidation of 7-DHC into its endoperoxide form by singlet oxygen may account for the enhanced therapeutic effect, realizing an efficient combination of photodynamic therapy (PDT) and photoactivated chemotherapy.


Assuntos
Antineoplásicos/farmacologia , Desidrocolesteróis/farmacologia , Lipossomos/farmacologia , Fármacos Fotossensibilizantes/farmacologia , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Desidrocolesteróis/química , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Lipossomos/química , Imagem Óptica , Tamanho da Partícula , Fotoquimioterapia , Fármacos Fotossensibilizantes/química , Espécies Reativas de Oxigênio/análise , Espécies Reativas de Oxigênio/metabolismo
11.
Orthop J Sports Med ; 7(10): 2325967119879052, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31656824

RESUMO

Background: Insertional Achilles tendinopathy (IAT) is a common finding in the clinic. However, consensus on its mechanism, pathological process, diagnosis, treatment, and rehabilitation is lacking. Thus, the Chinese Society of Sports Medicine organized and invited experts representing the fields of ankle disease and tendinopathy to jointly develop an expert consensus on IAT. Study Design: A consensus statement of the Chinese Society of Sports Medicine. Methods: A total of 34 experts in the field of sports medicine and orthopaedics were invited to participate in the compilation of a consensus statement regarding IAT. Consensus was achieved according to the Delphi method. First, 10 working groups composed of 34 experts were established to compile draft statements about clinical problems related to IAT by reviewing and analyzing the available literature. An expert consensus meeting to discuss drafts was then arranged. Each statement was individually presented and discussed, followed by a secret vote. Consensus was reached when more than 50% of the experts voted in its favor. The strength of the proposed recommendation was classified based on the proportion of favorable votes: consensus, 51% to 74%; strong consensus, 75% to 99%; unanimity, 100%. Results: Of the 10 expert consensus statements on the clinical diagnosis and treatment of IAT, there was strong consensus for 8 statements and unanimity for 2 statements. Conclusion: This expert consensus focused on the concepts, causes, pathological process, clinical diagnosis, and treatment of IAT. Accepted recommendations in these areas which will assist clinicians in carrying out standardized management of related diseases.

12.
Zhongguo Zhen Jiu ; 39(10): 1063-6, 2019 Oct 12.
Artigo em Chinês | MEDLINE | ID: mdl-31621258

RESUMO

OBJECTIVE: To observe the therapeutic effect of pre-acupuncture at Neiguan (PC 6) and Zusanli (ST 36) on exercise-induced fatigue. METHODS: A total of 50 subjects were divided into an observation group and a control group by random number table method, 25 cases in each one. In the observation group, acupuncture was applied at Neiguan (PC 6) and Zusanli (ST 36) before exercise, once a day, consecutive 5-day treatment was required. In the control group, there was no intervention. The subjective fatigue perception score, serum levels of lactate and lactate dehydrogenase, heart rate and oxygen saturation before and after exercise were observed in the two groups. RESULTS: Compared before exercise, the subjective fatigue perception scores, serum levels of lactate and lactate dehydrogenase and heart rates after exercise were increased in the two groups (P<0.05, P<0.01), and the subjective fatigue perception score, serum levels of lactate and lactate dehydrogenase and heart rate after exercise in the observation group were lower than the control group (P<0.05, P<0.01). There was no significant difference in oxygen saturation before and after exercise in the two groups,there was no significant difference in oxygen saturation after exercise between the two groups (P>0.05). CONCLUSION: Pre-acupuncture at Neiguan (PC 6) and Zusanli (ST 36) can effectively reduce the subjective fatigue perception score,serum levels of lactate and lactate dehydrogenase and heart rate to improve exercise-induced fatigue.


Assuntos
Pontos de Acupuntura , Terapia por Acupuntura , Exercício , Fadiga/terapia , Estudos de Casos e Controles , Exercício/fisiologia , Frequência Cardíaca , Humanos
13.
Orthop J Sports Med ; 7(9): 2325967119873852, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31579683

RESUMO

Background: The surgical management of chronic lateral ankle instability (CLAI) has evolved since the 1930s, but for the past 50 years, the modified Broström technique of ligament repair has been the gold standard. However, with the development of arthroscopic techniques, significant variation remains regarding when and how CLAI is treated operatively, which graft is the optimal choice, and which other controversial factors should be considered. Purpose: To develop clinical guidelines on the surgical treatment of CLAI and provide standardized guidelines for indications, surgical techniques, rehabilitation strategies, and assessment measures for patients with CLAI. Study Design: A consensus statement of the Chinese Society of Sports Medicine. Methods: A total of 14 physicians were queried for their input on guidelines for the surgical management of CLAI. After 9 clinical topics were proposed, a comprehensive systematic search of the literature published since 1980 was performed for each topic through use of China Biology Medicine (CBM), China National Knowledge Infrastructure (CNKI), PubMed, Web of Science, EMBASE, and the Cochrane Library. The recommendations and statements were drafted, discussed, and finalized by all authors. The recommendations were graded as grade 1 (strong) or 2 (weak) based on the GRADE (Grading of Recommendations Assessment, Development, and Evaluation) concept. Based on the input from 28 external specialists independent from the authors, the clinical guidelines were modified and finalized. Results: A total of 9 topics were covered with regard to the following clinical areas: surgical indications, surgical techniques, whether to address intra-articular lesions, rehabilitation strategies, and assessments. Among the 9 topics, 6 recommendations were rated as strong and 3 recommendations were rated as weak. Each topic included a statement about how the recommendation was graded. Conclusion: This guideline provides recommendations for the surgical management of CLAI based on the evidence. We believe that this guideline will provide a useful tool for physicians in the decision-making process for the surgical treatment of patients with CLAI.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31514158

RESUMO

Broad learning system (BLS) is a novel neural network with effective and efficient learning ability. BLS has attracted increasing attention from many scholars owing to its excellent performance. This article proposes a weighted BLS (WBLS) based on BLS to tackle the noise and outliers in an industrial process. WBLS provides a unified framework for easily using different methods of calculating the weighted penalty factor. Using the weighted penalty factor to constrain the contribution of each sample to modeling, the normal and abnormal samples were allocated higher and lower weights to increase and decrease their contributions, respectively. Hence, the WBLS can eliminate the bad effect of noise and outliers on the modeling. The weighted ridge regression algorithm is used to compute the algorithm solution. Weighted incremental learning algorithms are also developed using the weighted penalty factor to tackle the noise and outliers in the additional samples and quickly increase nodes or samples without retraining. The proposed weighted incremental learning algorithms provide a unified framework for using different methods of computing weights. We test the feasibility of the proposed algorithms on some public data sets and a real-world application. Experiment results show that our method has better generalization and robustness.

15.
Neural Regen Res ; 14(12): 2164-2172, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31397356

RESUMO

Quantitative assessment of the recovery of nerve function, especially sensory and autonomic nerve function, remains a challenge in the field of nerve regeneration research. We previously found that neural control of vasomotor activity could be potentially harnessed to evaluate nerve function. In the present study, five different models of left sciatic nerve injury in rats were established: nerve crush injury, nerve transection/suturing, nerve defect/autografting, nerve defect/conduit repair, and nerve defect/non-regeneration. Laser Doppler perfusion imaging was used to analyze blood perfusion of the hind feet. The toe pinch test and walking track analysis were used to assess sensory and motor functions of the rat hind limb, respectively. Transmission electron microscopy was used to observe the density of unmyelinated axons in the injured sciatic nerve. Our results showed that axonotmesis-evoked vasodilatation in the foot 6 months after nerve injury/repair recovered to normal levels in the nerve crush injury group and partially in the other three repair groups; whereas the nerve defect/non-regeneration group exhibited no recovery in vasodilatation. Furthermore, the recovery index of axonotmesis-evoked vasodilatation was positively correlated with toe pinch reflex scores and the density of unmyelinated nerve fibers in the regenerated nerve. As C-fiber afferents are predominantly responsible for dilatation of the superficial vasculature in the glabrous skin in rats, the present findings indicate that axonotmesis-evoked vasodilatation can be used as a novel way to assess C-afferent function recovery after peripheral nerve injury. This study was approved by the Ethics Committee for Laboratory Animals of Nantong University of China (approval No. 20130410-006) on April 10, 2013.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31398131

RESUMO

In recent years, the deep reinforcement learning (DRL) algorithms have been developed rapidly and have achieved excellent performance in many challenging tasks. However, due to the complexity of network structure and a large amount of network parameters, the training of deep network is time-consuming, and consequently, the learning efficiency of DRL is limited. In this paper, aiming to speed up the learning process of DRL agent, we propose a novel approximate policy-based accelerated (APA) algorithm from the viewpoint of the error analysis of approximate policy iteration reinforcement learning algorithms. The proposed APA is proven to be convergent even with a more aggressive learning rate, making the DRL agent have a faster learning speed. Furthermore, to combine the accelerated algorithm with deep Q-network (DQN), Double DQN and deep deterministic policy gradient (DDPG), we proposed three novel DRL algorithms: APA-DQN, APA-Double DQN, and APA-DDPG, which demonstrates the adaptability of the accelerated algorithm with DRL algorithms. We have tested the proposed algorithms on both discrete-action and continuous-action tasks. Their superior performance demonstrates their great potential in the practical applications.

17.
Accid Anal Prev ; 132: 105268, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31465932

RESUMO

Single-vehicle (SV) and multi-vehicle (MV) crashes have been recognized as differing in spatial distribution and influencing factors, but little consideration has been given to these differences as related to hotspot identification. For the purpose of better hotspot identification, this study aims to analyze influencing factors of SV and MV crashes and to explore the consistency between SV and MV hotspots. Crash data, roadway geometric design features, and traffic characteristics were collected along the two directions of a 45-km freeway section in Shanghai, China. Univariate negative binomial conditional autoregressive (NB-CAR) and bivariate negative binomial spatial conditional autoregressive (BNB-CAR) models were developed to analyze the influencing factors and specifically address (1) site correlation between SV and MV crashes within the same freeway segment, and (2) spatial correlation among different freeway segments within the same direction. The modeling results showed substantial differences in the significant factors that influence SV and MV crashes, including both roadway geometric features and traffic operational factors. A non-negligible site correlation was found between SV and MV crashes. Taking into account the site correlation, the BNB-CAR model outperformed the NB-CAR model in terms of parameter estimation and model fitting. For hotspot identification, potential for safety improvement based on the empirical Bayes method was adopted to handle the crash fluctuation problem. Substantial inconsistency was found between SV and MV hotspots despite the site correlation: in the top ten hotspots, no hotspot was shared by the two crash types. This result highlights the importance of differentiating SV and MV crashes when identifying hotspots, providing insight into freeway safety analysis.


Assuntos
Acidentes de Trânsito/estatística & dados numéricos , Ambiente Construído , Teorema de Bayes , China , Humanos , Modelos Estatísticos , Reprodutibilidade dos Testes
18.
Chemistry ; 25(61): 13879-13884, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31468605

RESUMO

Ruthenium(II) polypyridyl complexes featuring peripheral quaternary ammonium structures were found to be able to selectively inactivate Gram-positive Staphylococcus aureus (S. aureus), including methicillin-resistant S. aureus (MRSA) upon visible light irradiation, but have low phototoxicity toward 293T cells, L02 cells and lack hemolysis toward rabbit red blood cells (RBC), exhibiting promising potential as a novel type of antimicrobial photodynamic therapy (aPDT) agents.

19.
Dalton Trans ; 48(32): 12177-12185, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31334521

RESUMO

Ru(ii) polypyridine complexes which can undergo photo-induced ligand dissociation and subsequent DNA covalent binding may potentially serve as photoactivated chemotherapeutic (PACT) agents. In this paper, three fluorinated dppz ligand coordinated Ru(ii) complexes (2-4) containing four monodentate pyridine ligands were studied. All complexes released one pyridine and covalently bound to DNA upon 470 nm irradiation. Compared with the parent complex [Ru(dppz)(py)4]2+ (1), 2-4 displayed enhanced phototoxicity but diminished dark cytotoxicity, more favorable for PACT application. Complex 3 is the most efficient one with IC50 values of about 8 µM toward HeLa and SKOV-3 cell lines, and also has a much higher IC50 value toward normal L-02 cells. Our results indicate that fluorination on the retaining ligand may be an efficient way to improve the drug activity of Ru(ii) PACT agents.

20.
J Agric Food Chem ; 67(33): 9423-9431, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31329442

RESUMO

A high-throughput lipid analysis method was established to comprehensively investigate the lipid profiles of three marine (Scomberomorus niphonius, Scophthalmus maximus, and Oncorhynchus keta) and one freshwater (Ctenopharyngodon idellus) fish species using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Over 700 molecular species from 12 major lipid subclasses were identified. Glycerolipids (73.7-85.6%) and phospholipids (PLs, 13.7-25.6%) were dominant components in total lipids. Polyunsaturated fatty acid PLs, such as phosphatidylethanolamine (PE, 16:0-22:6), PE (18:1-22:6), and phosphatidylcholine (16:0-22:6), were the major molecular species in PLs. The lipid composition of three marine fish (mainly C22:5) was significantly different from that in C. idellus (mainly C20:4 and C20:5). A unique long-chain base of sphingolipids was found in fish (mainly d19:3). These bioactive lipids were proposed to be potential biomarkers for distinguishing different fish species and evaluating nutritional values.


Assuntos
Lipídeos/química , Alimentos Marinhos/análise , Animais , Cromatografia Líquida de Alta Pressão , Cyprinidae , Ácidos Graxos Insaturados/química , Linguados , Espectrometria de Massas , Oncorhynchus keta , Fosfatidiletanolaminas/química , Fosfolipídeos/química , Atum
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