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1.
Br J Surg ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34643677

RESUMO

BACKGROUND: The therapeutic value of repeat hepatic resection (rHR) or radiofrequency ablation (RFA) for recurrent hepatocellular carcinoma (HCC) is unknown. This study aimed to investigate the safety and efficacy of rHR or RFA. METHODS: This was a retrospective multicentre study of patients with recurrent HCC within the Milan criteria who underwent rHR or RFA at nine university hospitals in China and Italy between January 2003 and January 2018. Survival after rHR or RFA was examined in unadjusted analyses and after propensity score matching (1 : 1). RESULTS: Of 847 patients included, 307 and 540 underwent rHR and RFA respectively. Median overall survival was 73.5 and 67.0 months after rHR and RFA respectively (hazard ratio 1.01 (95 per cent c.i. 0.81 to 1.26)). Median recurrence-free survival was longer after rHR versus RFA (23.6 versus 15.2 months; hazard ratio 0.76 (95 per cent c.i. 0.65 to 0.89)). These results were confirmed after propensity score matching. RFA was associated with lower morbidity of grade 3 and above (0.6 versus 6.2 per cent; P < 0.001) and shorter hospital stay (8.0 versus 3.0 days, P < 0.001) than rHR. CONCLUSION: rHR was associated with longer recurrence-free survival but not overall survival compared with RFA.

2.
Zhonghua Wei Chang Wai Ke Za Zhi ; 24(10): 897-903, 2021 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-34674465

RESUMO

Objective: Splenic flexure mobilization is technically difficult during the resection of left hemicolon cancer. This study aims to compare the safety and effectiveness between the bursa omentalis approach (BOA) and medial-to-lateral approach (MTLA) in laparoscopic radical resection of left-sided colon cancer. Methods: A retrospective cohort study was conducted. Inclusion criteria: (1) patients underwent radical resection of left hemicolon cancer; (2) the postoperative pathological result was adenocarcinoma; (3) patients aged 18-80 years old; (4) no liver, peritoneal or other distant metastasis. Exclusion criteria: (1) poor physical condition with serious heart, brain, lung, liver, kidney and hematopoietic system diseases; (2) unable to tolerate laparoscopic surgery; (3) history of other malignancies simultaneously, or multisource tumors; (4) emergency operation due to bleeding, obstruction, perforation, etc. Clinical data of 189 patients who underwent laparoscopic left hemicolectomy in the Guangdong Provincial Hospital of Chinese Medicine from 2014 to 2020 were retrospectively analyzed. According to surgical approaches, patients were divided into the BOA group (52 cases) and MTLA group (137 cases). The whole group of patients were matched by propensity score matching (PSM) according to the nearest neighbor matching method. The caliper value was 0.01. The matching variables included gender, age, American Society of Anesthesiologists (ASA) score, body mass index, tumor location and tumor stage. After PSM, 47 patients were included in the BOA group and MTLA group, respectively. There were no significant differences in baseline data between the two groups after PSM (all P>0.05). Paired t-test, paired rank sum test and paired Chi-square test were used to compare intraoperative and postoperative paramether between the two groups. Kaplan-Meier method was used to draw the survival curve, and log rank test was used for inter group comparison. When the two survival curves intersect, the two-stage method and restricted mean survival time (RMST) were further performed. Results: Both groups of patients successfully completed the operation without conversion to laparotomy or intraoperative death. No combined splenectomy or pancreatectomy were performed in the two groups. There were also no significant differences in intraoperative blood loss, number of harvested lymph nodes, time to the first flatus and the length of hospital stay between the two groups (all P>0.05). However, the median laparoscopic dissection time in the BOA group was shorter than that in the MTLA group, and the difference was statistically significant (median: 56 minutes vs. 65 minutes, P=0.032). No entry to posterior pancreatic space was recorded in the BOA group but wrong entry to posterior pancreatic space happened to 6.4% (3/47) of patients (body mass index >25 kg/m(2)) when dissecting left Toldt's fascia in the MTLA group. The 3-year disease-free survival rate in BOA group and MTLA group was 90.2% and 86.1%, respectively (P=0.909) and the 3-year overall survival rate was 85.6% and 94.4%, respectively (P=0.532). Conclusions: BOA is safe and feasible in laparoscopic left hemicolectomy, especially for inexperienced surgeons. For obese patients, BOA facilitates the entrance into the correct anatomical level and avoid entering the retropancreatic space.

3.
Zhonghua Nei Ke Za Zhi ; 60(7): 661-664, 2021 Jul 01.
Artigo em Chinês | MEDLINE | ID: mdl-34619844

RESUMO

To investigate the predictive value of [18F]fluorodeoxyglucose-positron emission computed tomography(PET)/CT for disease progression in patients with dermatomyositis (DM) and interstitial lung diseases (ILD). Sixty-seven DM patients who underwent [18F] FDG-PET/CT imaging were retrospectively analyzed from January 2012 to September 2017 at PLA General Hospital. Their clinical manifestations and imaging characteristics were recorded. Compared with those chronically progressed (C-ILD), patients with rapid progression (RP-ILD) had significantly higher erythrocyte sedimentation rate (ESR) and standardized uptake value (SUV) in lungs (P<0.05). In patients with RP-ILD, SUV in lungs was positively correlated with age, disease course, and ESR. Receiver operating characteristic curve analysis suggested that when lung SUV cut off value was 2.25, the sensitivity and specificity to predict disease progression was 77.8% and 72.8%, respectively. Old age, longer disease course, low creatine kinase level, higher ESR, and high SUV are prognostic factors for DM-associated ILD.


Assuntos
Dermatomiosite , Doenças Pulmonares Intersticiais , Dermatomiosite/complicações , Dermatomiosite/diagnóstico por imagem , Progressão da Doença , Elétrons , Fluordesoxiglucose F18 , Humanos , Doenças Pulmonares Intersticiais/diagnóstico por imagem , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Estudos Retrospectivos
4.
Zhonghua Xin Xue Guan Bing Za Zhi ; 49(9): 912-919, 2021 Sep 24.
Artigo em Chinês | MEDLINE | ID: mdl-34530600

RESUMO

Objective: To investigate the effect of neuregulin-1(NRG-1) on cardiac glucose metabolism in Sprague Dawley (SD) rats with experimental myocardial infarction (MI). Methods: Adult male SD rats were randomly divided into three groups: the sham-operated group, MI group, and MI+NRG1 group. The rat MI model was established via ligation of the left anterior descending coronary artery. Two weeks after operation, echocardiography was performed, MI rats with left ventricular ejection fraction (LVEF) between 0.3-0.5 were selected and randomly assigned to MI group and MI+NRG-1 group. Rats in MI+NRG-1 group were treated with recombinant human NRG-1ß (100 µg/kg) via tail vein at 2 weeks after operation (twice per week for 6 weeks); while rats in sham-operated group and MI group received equal volume of physiological saline. By the end of administration, echocardiography and small animal positron emission tomography (PET) were performed to detect cardiac function and myocardial glucose uptake. Myocardial morphology and collagen volume fraction, cardiomyocyte apoptosis and reactive oxygen species (ROS) production were evaluated by histopathologic analysis. Myocardial pyruvate dehydrogenase (PDH) and citrate synthase (CS) activity, as well as ATP production were detected by commercial kits. The mRNA and protein expression levels of NRG-1, p-ErbB4, and key factors involved in glucose metabolism (including Glut-4, HK2, PDK4, PDH, CS) were detected by quantitative real-time PCR (qRT-PCR) and Western blot assay, respectively. Results: With the MI model successfully established, the left ventricular ejection fraction(LVEF) and left ventricular shortening fraction(LVFS) were significantly lower in MI group and MI+NRG-1 group than that in sham group (both P<0.01), while there was no significant difference between MI group and MI+NRG-1 group(all P>0.05). After 6 weeks of NRG-1ß intervention, the LVEF and LVFS were significantly higher in MI+NRG-1 group than in MI group (both P<0.01). By the end of experiment, PET imaging showed that the mean standardized uptake value (SUVmean) were lower in MI+NRG-1 group than in the sham group (4.06±0.28 vs. 5.18±0.37, P<0.01), while significantly higher than that in MI group (4.06±0.28 vs.2.86±0.49, P<0.01). Histopathological analysis showed that compared with MI group, rats in MI+NRG-1 group exhibited significantly decreased left ventricle collagen volume fraction ((7.83±1.24) % vs. (18.31±3.58) %, P<0.01), cardiomyocyte apoptosis((37.98±4.26)% vs. (67.04±5.38)%, P<0.01), and DHE fluorescence intensity(0.057 28±0.007 06 vs. 0.076 94±0.008 46, P<0.01), indicating that NRG-1ß could reduce ROS production. PDH activity, CS activity, and ATP production were significantly higher in MI+NRG-1 group than in MI group (all P<0.05). qRT-PCR demonstrated an upregulated Glut-4, HK2 and CS, but downregulated PDK4 mRNA expression in MI+NRG-1 group compared with MI group (all P<0.01). Western blot assay showed significantly higher protein expression of NRG-1, p-ErbB4, Glut-4, HK2, PDH, CS in MI+NRG-1 group than in MI group (all P<0.01). Conclusion: NRG-1 could improve glucose uptake and utilization in myocardium by activating phosphorylation of myocardial ErbB4 receptor in MI rats, thus providing a therapeutic option for improving energy metabolism after MI.


Assuntos
Infarto do Miocárdio , Neuregulina-1 , Animais , Glucose , Masculino , Infarto do Miocárdio/tratamento farmacológico , Miocárdio , Ratos , Ratos Sprague-Dawley , Volume Sistólico , Função Ventricular Esquerda
5.
Zhonghua Yi Xue Za Zhi ; 101(34): 2698-2702, 2021 Sep 14.
Artigo em Chinês | MEDLINE | ID: mdl-34510876

RESUMO

Objective: To investigate the clinical effect of the radical resection with a proximal incisal edge length of 20-25 mm and 30-35 mm in Siewert type Ⅱ advanced esophagogastric junction adenocarcinoma, to shorten the minimum safe distance of the proximal incisal edge to 20-25 mm. Methods: A retrospective cohort study method was used. The clinical data of 166 patients with Siewert type Ⅱ advanced esophagogastric junction adenocarcinoma who underwent total gastrectomy from January 2017 to August 2020 in the Department of Gastrointestinal Surgery, Heji Hospital Affiliated to Changzhi Medical College were retrospectively collected. According to the proximal incisal edge length, the patients were divided into two groups: the proximal incisal edge length of 20-25 mm group (69 cases) and 30-35 mm group (97 cases). The perioperative conditions and the 6-month follow-up after the operation were compared between the two groups. Results: There was no statistically significant difference in baseline information between the patients in the two groups (P>0.05). The operations of both groups were completed. The intraoperative operation time of the proximal incisal edge length of 20-25 mm group was shorter than that in the proximal incisal edge length of 30-35 mm group ((172±24)and(206±27)min, P<0.001). There were no significant differences in the amount of intraoperative blood loss, the treatment of the diaphragm during the operation and the positive rate of intraoperative freezing of the upper incisal edge between the patients in the two groups (all P>0.05). And there was no significant differences in the first exhaust time, gastric tube removal time, first feeding time and hospital stay after the operation of the two groups (all P>0.05). There was no significant differences in the incidence of anastomotic leakage, anastomotic stenosis, reflux esophagitis and intestinal obstruction after the operation between the patients in the two groups (all P>0.05). And there was no anastomotic leakage case among the 69 cases in the proximal incisal edge length of 20-25 mm group. Postoperative pathological treatment showed no significant differences in the vascular tumor thrombus and nerve infiltration between the two groups (both P>0.05). During the 6-month follow-up, there was no death or tumor recurrence in the two groups, and there was no significant difference in body weight loss at 6 months after the operation between the two groups (P=0.178). Conclusion: When radical resection of Siewert type Ⅱ advanced esophagogastric junction adenocarcinoma is performed, it is feasible to shorten the minimum safe distance of the proximal incisal edge to 20-25 mm under the premise of ensuring R0 resection. The operation time is shortened. Due to the shortening the incisal edge distance, the anastomotic tension is decreased, and the incidence of postoperative anastomotic leakage is also reduced.


Assuntos
Adenocarcinoma , Neoplasias Esofágicas , Neoplasias Gástricas , Adenocarcinoma/cirurgia , Neoplasias Esofágicas/cirurgia , Junção Esofagogástrica , Gastrectomia , Humanos , Recidiva Local de Neoplasia , Estudos Retrospectivos , Neoplasias Gástricas/cirurgia
6.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(9): 873-879, 2021 Sep 09.
Artigo em Chinês | MEDLINE | ID: mdl-34496535

RESUMO

Objective: To record occlusal contact of primary dentition at maximal intercuspal position in children with individual normal occlusion. Methods: A total of 57 children were recruited from patients of the Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology. Inclusion criteria were that the subjects were 3-5 years old with no visually detectable caries or pupal and periapical diseases, had complete primary dentition, had individual normal occlusion, had normal function of craniofacial system, were medically healthy, could cooperate with sampling and had obtained written informed consent from the parents or guardians. Finally, forty-seven children aged 3 to 5 years old were enrolled, including 24 males and 23 females. The age, height and weight of all subjects were (4.1±0.7) years old (ranging 3.0-5.8 years old), (103.7±7.2) cm (ranging 90-120 cm) and (17.1±2.5) kg (ranging 12.5-22.5 kg), respectively. Occlusal abilities such as occlusal contact area, average bite pressure, maximum bite pressure, maximum bite force and occlusal balance were measured with Dental Prescale Ⅱ system. Results: Maximum bite force and occlusal contact area at intercuspal position in children with primary dentition were (567.40±223.84) N (ranging 226.7-1 154.6 N) and (18.56±6.54) mm2 (ranging 8.4-41.2 mm2), respectively. There was a significantly strong correlation between maximum bite force and occlusal contact area (r=0.954, P<0.01). Height and weight of children were also positively correlated with their maximum bite force (r=0.397, P=0.022 and r=0.453, P=0.008, respectively). Maximum occlusal bite force and contact occlusal area of boys [(651.80±224.34) N and (20.77±6.97) mm2] were significantly higher and larger than those of girls [(479.34±190.45) N and (16.25±5.27) mm2] (P<0.05). Thirty-two of all 47 children had one occlusal contact point with maximum bite pressure, mostly locating within the primary molar region. Bite forces of anterior and posterior teeth of primary dentition were (124.12±56.99) N and (450.11±205.09) N, respectively, about (21.82±11.40)% and (71.80±21.35)% of maximum bite force of the whole primary dentition. All of the occlusal balance points located in posterior teeth regions. Occlusal contacts were observed at both anterior and posterior teeth of primary dentition with individual normal occlusion. Conclusions: There was a great variation of maximum bite forces of primary dentitions at intercuspal position of children with individual normal occlusion. Maximum bite force of primary dentition was significantly correlated with occlusal contact area, height and weight of children. Occlusal contact points with maximum bite pressure and occlusal bite balance points of primary dentitions mostly located in primary molar regions.


Assuntos
Força de Mordida , Oclusão Dentária , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Dente Molar , Dente Decíduo
7.
Fa Yi Xue Za Zhi ; 37(3): 388-395, 2021 Jun.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34379910

RESUMO

Abstract: In cases of sudden death, the prevention of sudden cardiac death and the analysis of the cause of death after sudden cardiac death have always been a difficult problem. Therefore, clinical research and forensic pathological identification of sudden cardiac death are of great significance. In recent years, metabolomics has gradually developed into a popular field of life science research. The detection of "metabolic fingerprints" of biological fluids can provide an important basis for early diagnosis of diseases and the discovery of potential biomarkers. This article reviews the current research status of sudden cardiac death and the research on metabolomics of cardiovascular diseases that is closely related to sudden cardiac death and analyzes the application prospects of metabolomics in the identification of the cause of sudden cardiac death.


Assuntos
Morte Súbita Cardíaca , Metabolômica , Biomarcadores , Morte Súbita Cardíaca/etiologia , Morte Súbita Cardíaca/prevenção & controle , Patologia Legal , Humanos
8.
Artigo em Chinês | MEDLINE | ID: mdl-34218562

RESUMO

Objective: To observe the clinical characteristics of occupational chronic fluorosis cases and follow up patients'condition changes after they have been transferred from fluoride work. Methods: In January 2020, the clinical data of occupational chronic fluorosis cases diagnosed in our hospital and their corresponding two-year follow-up data were collected and analyzed. These cases happened in an aluminum factory between January 2010 to January 2020. Results: Nine patients were diagnosed as occupational chronic mild fluorosis because of long bones and joints pain. Skeletal X-ray examination showed a result of skeletal fluorosis and increased urine fluoride. Among the six patients who have been followed up for two years, two of them showed improvements in both symptoms and skeletal X-ray examination results. Three patients'symptoms were alleviated, but their skeletal X-ray examination results showed no significant change. Besides them, the last patient's symptoms and skeletal X-ray examination results were aggravated, which was considered related to still exposed to hydrogen fluoride in new position. Conclusion: Changes of skeletal X-ray examination are the characteristics of occupational chronic fluorosis. By leaving the fluoride position, patients'clinical symptoms can be alleviated, and their bone lesions are not aggravated or even show a trend of alleviating in some cases.


Assuntos
Alumínio , Doenças Ósseas , Fluoretos , Seguimentos , Humanos , Radiografia
9.
Zhonghua Gan Zang Bing Za Zhi ; 29(6): 571-574, 2021 Jun 20.
Artigo em Chinês | MEDLINE | ID: mdl-34225433

RESUMO

Objective: To investigate the expression of miR-495 and its effect on MHCC-97H hepatocellular carcinoma cells. Methods: Fifty-six hepatocellular carcinoma tissue specimens (HCC group) and 40 normal liver tissue specimens (control group) preserved in our hospital from January 2017 to January 2018 were selected. Reverse transcription real-time fluorescent quantitative PCR (qRT-PCR) was used for miR-495 expression detection. MHCC-97H HCC cells were randomly selected and then divide into control group, blank plasmid group and transfection group. The blank plasmid group was transfected with blank plasmid, and the transfection group was transfected with miR-495 inhibitor. The expression of miR-495 in each group of cells were detected using qRT-PCR. CCK method was used to detect each group proliferation activity. Transwell cell migration assay was used to detect each group migration ability. Analysis of variance was used for comparison between multiple groups. Furthermore, LDS-t test was used for pairwise comparison, and t -test was used for comparison between the two groups. Results: The relative expression levels of miR-495 in the HCC group was (2.043 ± 0.382), which was higher than the control group, and the difference between the two groups was statistically significant (P < 0.05). The relative expressions levels of miR-495 in patients with stage III to IV and lymph node metastasis were 2.265 ± 0.284 and 2.290 ± 0.355, which were significantly higher than those of stage I to II and no lymph node metastasis (P < 0.05). The relative expression levels of miR-495 in transfection group was 0.653 ± 0.102, which were significantly lower than control group and blank plasmid group (P < 0.05). The A values of MHCC-97H cells cultured for 24 h and 48 h in transfection group were 0.404 ± 0.106 and 0.604 ± 0.136, which were significantly lower than control group and blank plasmid group (P < 0.05). MHCC-97H cells migration number in the transfection group was (6.10 0 ± 20), which was significantly lower than that of control group and blank plasmid group (P < 0.05). Conclusion: miR-495 high expression has certain relationship with clinicopathological characteristics of HCC tissues. In addition, miR-495 has a certain effect on the proliferation and migration ability of MHCC-97H HCC cells.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , MicroRNAs , Carcinoma Hepatocelular/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/genética , MicroRNAs/genética
10.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(6): 525-531, 2021 Jun 09.
Artigo em Chinês | MEDLINE | ID: mdl-34098667

RESUMO

Objective: To determine the ratio of ingerleukin(IL)-10 secreting regulatory B cells (B10 cells) and the levels of IL-10 in peripheral blood of periodontitis patients with or without chronic obstructive pulmonary disease (COPD), and to explore the function of B10 cells in the pathogenesis of the interaction between COPD and periodontitis. Methods: Forty-five periodontitis patients and healthy people were recruited and divided into three groups: COPD group, COPD+periodontitis group and health controls with 15 people in each group. Peripheral blood samples were collected to determine the B10 population. Another group of 93 periodontitis patients and healthy people were also collected and divided into the same 3 groups with 31 people in each group. Peripheral blood samples were collected to determine the cytokine IL-10. Results: After stimulation with lipopolysaccharide (LPS)+phorbol-12-myristate13-acetate+ionomycin+brefeldin A (PIB) for 5 h, the ratio of B10 in COPD+periodontitis group [(0.44±0.11)%] was significantly lower than that in the control group [(0.63±0.14)%] and periodontitis group [(0.62±0.13)%] (P<0.01), respectively. The ratio of B10 cells showed no significant difference between periodontitis group and healthy control group (P>0.05). After stimulation with LPS+PIB for 48 h, the ratio of B10 cells in COPD+periodontitis group [(7.59±1.33)%] was significantly lower than that in the periodontitis group [(10.14±2.02)%] and the healthy controls [(11.80±1.71)%] (P<0.01), respectively. The ratio of B10 cells in periodontitis group was significantly lower than that in the healthy controls (P<0.05). The levels of IL-10 in periodontitis group [(2.55±0.61) ng/L] and COPD+periodontitis group [(1.95±0.45) ng/L] were significantly lower than that in the control group [(3.96±1.15) ng/L] (P<0.01) respectively. Moreover, the level of IL-10 in COPD+periodontitis group was significantly lower than that in the periodontitis group (P<0.01). Conclusions: The dysfunction of immune system caused by the decreased proportion of B10 cells might be involved in the pathological process of COPD promoted by periodontitis.


Assuntos
Doenças Periodontais , Periodontite , Doença Pulmonar Obstrutiva Crônica , Citocinas , Humanos , Lipopolissacarídeos
11.
Fa Yi Xue Za Zhi ; 37(2): 145-150, 2021 Apr.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34142473

RESUMO

Abstract: Objective To investigate the correlation between the polymorphism of 4 coagulation-related genes, rs1799963 (coagulation factor V gene Leiden), rs6025 (prothrombin gene G20210A), rs1042579 (thrombomodulin protein gene c.1418C>T) and rs1801131 (methylenetetrahydroflate reductase gene) and lower extremity deep venous thrombosis (LEDVT). Methods The 4 genotypes mentioned above of 150 LEDVT patients and 153 healthy controls were detected by the kompetitive allele specific polymerase chain reaction (KASP), then related blood biochemical indicators were collected, binary Logistic regression was established to screen the independent risk factors of LEDVT, and the correlation between polymorphism of 4 coagulation-related genes and LEDVT and its indicators under different genetic modes after adjusting confounding factors were analyzed. Results Five variables, D-dimer, fibrinogen degradation product, homocysteine, sex and age might be the risk factors of LEDVT. These variables were put into 4 genetic inheritance models, and adjusted in binary Logistic regression. The results suggested that the mutations of rs1042579 were correlated with LEDVT under dominant inheritance mode. Conclusion The gene polymorphism of rs1799963, rs6025 and rs1801131 has no significant correlation with the formation of LEDVT. The gene polymorphism of rs1042579 plays a role under dominant inheritance mode, and might be an independent risk factor for formation of LEDVT.


Assuntos
Trombose Venosa , Coagulação Sanguínea/genética , Humanos , Extremidade Inferior , Polimorfismo Genético , Fatores de Risco , Trombose Venosa/genética
13.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(5): 428-434, 2021 May 09.
Artigo em Chinês | MEDLINE | ID: mdl-33904276

RESUMO

Objective: To investigate the clinical effect of free fibula flap transplantation in repairing the defect of mandibular osteoradionecrosis (ORN). Methods: A total of 151 mandibular ORN patients undergoing free fibular flap transplantation were selected from August 2005 to September 2020 in the Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University. Among them, 109 patients were males and 42 patients were females, aged (54.1±10.1) (ranged 31-85) years old. The clinical data of the patients was collected and the survival rate of the flaps and postoperative function were calculated to evaluate the surgical efficacy. The χ2 test was used for difference analysis. Results: Among the 151 patients, mandibular ORN caused by radiotherapy for nasopharyngeal carcinoma accounted for 79.5% (120/151). The average time for mandibular ORN appeared was 5(6) years after radiotherapy. Facial artery [57.2%(87/152)] and superior thyroid artery (50/152, 32.9%) were the main anastomotic arteries in the recipient area. There was no significant difference in the necrosis rates of the two flaps [10.3%(9/87) and 12.5%(5/50), respectively, P=0.949]. The main anastomotic veins in the recipient area were the external jugular vein [48.4%(135/279)] and the common facial vein [26.5%(74/279)]. Twenty-five cases (16.6%) had one vein anastomosed, and 126 cases (83.44%) had two veins anastomosed. There was no significant difference in the flap necrosis rate between the two conditions [20.0%(5/25) and 7.1%(9/126), respectively, P=0.100]. Ninety-seven cases (64.2%) used the peroneal musculocutaneous-fascia composite flap to repair the maxillofacial soft and hard tissue defects. Thirteen cases (8.6%) underwent the restorations with digital virtual surgery design, of which 5 cases were repaired with dental implants at the same time. After the operations, lower respiratory tract infection occurred in 17 patients (11.3%), and upper respiratory tract obstruction occurred in 3 cases (2.0%). The survival rate of the flap after operation was 90.7% (136/151), and 21 patients (13.9%) had flap vascular crisis. Delayed healing of maxillofacial wounds occurred in 33 cases (21.9%). After 3 to 24 months of follow-ups, 110 patients (76.9%) had no fistula inside/outside the oral cavity, 118 patients (82.5%) had an improvement in opening mouth of increasing (≥0.5 cm) after surgery, 135 patients (94.4%) had pain relief, 97 cases (67.8%) could eat normal diet, semi-liquid or soft food, and 137 cases (95.8%) were satisfied or basically satisfied with the treatment effects. Conclusions: The free fibular flap transplantation is an effective method to repair mandibular ORN defects. Preoperative vascular assessment is helpful for the selection of recipient vessels. Facial artery, superior thyroid artery, external jugular vein and common facial vein can be used as the main recipient vessels. The repair of the peroneal musculocutaneous-fascia composite flap facilitates the closure of internal and external fistulas. Digital technology can help to restore the maxillofacial shape more accurately, improve the patient's occlusal and chewing function and enhance the quality of life of mandibular ORN patients.

14.
Phys Rev Lett ; 126(11): 115001, 2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33798346

RESUMO

We report on a precision energy loss measurement and theoretical investigation of 100 keV/u helium ions in a hydrogen-discharge plasma. Collision processes of helium ions with protons, free electrons, and hydrogen atoms are ideally suited for benchmarking plasma stopping-power models. Energy loss results of our experiments are significantly higher than the predictions of traditional effective charge models. We obtained good agreement with our data by solving rate equations, where in addition to the ground state, also excited electronic configurations were considered for the projectile ions. Hence, we demonstrate that excited projectile states, resulting from collisions, leading to capture-, ionization-, and radiative-decay processes, play an important role in the stopping process in plasma.

15.
Artigo em Chinês | MEDLINE | ID: mdl-33660473

RESUMO

OBJECTIVE: To investigate the distribution and changing trend of canine echinococcosis in Inner Mongolia Autonomous Region, so as to provide the scientific evidence for prevention and control of canine echinococcosis in high-risk areas of Inner Mongolia Autonomous Region. METHODS: All data pertaining to Echinococcus infections in canine feces and sampling survey of human echinococcosis were collected from the echinococcosis-endemic foci of Inner Mongolia Autonomous Region from 2012 to 2018, and the prevalence of Echinococcus infections was investigated in dogs and humans. In addition, the spatial distribution characteristics and clusters of canine echinococcoses were identified. RESULTS: A total of 164 139 canine fecal samples were detected in the echinococcosis-endemic foci of Inner Mongolia Autonomous Region from 2012 to 2018, and there were 2 136 fecal samples positive for Echinococcus coproantigens. The positive rates of Echinococcus coproantigens were 0.54% to 1.73% in dogs from 2012 to 2018, with a tendency towards a decline seen in the prevalence of Echinococcus infections in dogs (χ2 = 108.83, P < 0.01), and there was a significant difference in the positive rate of Echinococcus coproantigens in dogs among years (χ2 = 155.27, P < 0.01). Three-dimensional trend analysis showed that canine echinococcosis was mainly concentrated in east of central Inner Mongolia Autonomous Region, and a high prevalence was detected in New Barag Right Banner and Sonid Right Banner. The global spatial distribution of canine echinococcosis appeared a random pattern in Inner Mongolia Autonomous Region from 2012 to 2018 (Moran's I > 0, P > 0.05), and there were "high-high" and "high-low" clusters of canine echinococcosis in local areas. The prevalence of human echinococcosis was 0.08%, and there was a significant difference in the prevalence among regions (χ2 = 147.61, P < 0.01), with a high prevalence seen in West Ujimqin Banner, Jarud Banner and New Barag Right Banner. In addition, the prevalence of human echinococcosis correlated positively with the Echinococcus coproantigen-positive rate in dogs (r = 0.52, P < 0.01). CONCLUSIONS: The prevalence of Echinococcus infections shows an overall tendency towards a decline in dogs in Inner Mongolia Autonomous Region from 2012 to 2018, with a high prevalence found in New Barag Right Banner and Sonid Right Banner. Human echinococcosis is concentrated in clusters of canine echinococcosis, where health education and targeted control interventions requires to be intensified.


Assuntos
Doenças do Cão , Equinococose , Echinococcus , Animais , China/epidemiologia , Doenças do Cão/epidemiologia , Cães , Equinococose/epidemiologia , Equinococose/veterinária , Fezes , Prevalência
16.
Fa Yi Xue Za Zhi ; 37(1): 69-76, 2021 Feb.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-33780188

RESUMO

Abstract: In the past, coronavirus caused two serious human-to-human pandemics in the world, including severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS). In late 2019, coronavirus disease 2019 (COVID-19) caused another major global public health event. Due to the strong infectivity of novel coronavirus, it is difficult to carry out the autopsy of related death cases widely. This paper reviews the previous status of the pathogen detection related to the autopsy of coronavirus infection diseases, and introduces the ongoing detection methods of novel coronavirus in clinical practice, in order to provide reference for the pathogen detection and study related to autopsy of COVID-19.


Assuntos
COVID-19 , Doenças Transmissíveis , Infecções por Coronavirus , Coronavírus da Síndrome Respiratória do Oriente Médio , Autopsia , Infecções por Coronavirus/diagnóstico , Humanos , SARS-CoV-2
18.
Br Poult Sci ; 62(4): 492-498, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33595370

RESUMO

1. The role of probiotics in modulating intestinal mucosal immunity in chicks was investigated by measuring migration of intraepithelial lymphocytes (IEL) and cytokine signals in chicks fed on a diet supplemented with the Lactobacillus casei compared with those of chicks fed on an unsupplemented diet.2. Increased numbers of intraepithelial lymphocytes (IEL) were detected in the ileal epithelium at d 3 and d 7 after feeding a diet containing 108 CFU/g L. casei.3. Greater expression of chemokine genes for C-C motif chemokine ligand 3, C-X-C motif chemokine ligand 12, C-C motif chemokine receptor 5, and C-C motif chemokine receptor 9 were detected in the ileum on d 3, suggesting a greater number of IEL was associated with lymphocyte migration through the chemokine signalling pathway.4. After IEL migration, cell proliferation was evident in mucosal epithelial cells on d 14. Evidence of immune responses induced in the ileum from d 3-21 after feeding the diet containing L. casei was shown by the significant (P < 0.05) differences in transforming growth factor-ß, secretory immunoglobulin A, interferon-γ, tumour necrosis factor-α, interleukin-4, and interleukin-10.5. These results indicated that feeding L. casei helps guide IEL migration and modulates intestinal mucosal immunity.


Assuntos
Linfócitos Intraepiteliais , Lactobacillus casei , Probióticos , Animais , Galinhas , Dieta/veterinária , Imunidade nas Mucosas , Mucosa Intestinal
19.
Fa Yi Xue Za Zhi ; 36(5): 660-665, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33295167

RESUMO

Abstract: Objective To obtain the protein expression profile of rat liver tissue after death by the 2100 bioanalyzer combined with protein chip, and infer the relationship between protein expression profile and postmortem interval. Methods Rats were killed by abdominal anesthesia and placed at 16 ℃. Water-soluble proteins in liver tissues were extracted at 14 time points after death. The expression profile data of proteins with relative molecular weight of 14 000-230 000 were obtained using protein chip, and principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and Fisher discriminant were used to analyze the data. Results According to the changes of protein expression profile, the postmortem interval was divided into group A (0 d), group B (1-9 d), group C (12-30 d) according to the result of PLS-DA. The prediction accuracy of the training set and test set of the model were all 100.0%, and the internal cross-validation of the training set was 100.0% according to Fisher discriminant. The Fisher discriminant model at each time point of group B and C was established to narrow the time window of postmortem interval estimation. The prediction accuracy of the training set and test set were all 100.0%, and the internal cross-validation accuracy of the training set was 100.0% in group B. The prediction accuracy of the training set and test set were respectively 95.2% and 78.6% in group C, and the internal cross-validation of the training set was 88.1%. Conclusion Protein chip detection technology can quickly and easily obtain the expression profile of water-soluble proteins of rat liver tissue with a relative molecular weight of 14 000-230 000 at different time points after death. PLS-DA and Fisher discriminant models are established to classify and predict the postmortem interval, in order to provide new ideas and methods for postmortem interval estimation.


Assuntos
Análise Serial de Proteínas , Tecnologia , Animais , Autopsia , Análise Discriminante , Análise dos Mínimos Quadrados , Mudanças Depois da Morte , Ratos
20.
Poult Sci ; 99(11): 5587-5597, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33142476

RESUMO

The aim of the research was to evaluate the dynamic changes of early posthatch starvation on residual yolk absorption, synthesis of macronutrients (protein, lipid, and glycogen), and organ development in broiler chicks. A total of 720 1-day-old chicks (Lingnan Yellow) were randomly assigned to 3 treatments: group A (nonfasted), group B (fasting for 24 h after placement), and group C (fasting for 48 h after placement). The trial lasted for 168 h, and water was provided ad libitum all the time. Sampling was performed at 0, 24, 48, 72, 120, and 168 h. Nonfasting (group A) promoted (P < 0.05) the absorption of amino acids, fatty acids, mineral elements, protein, and maternal antibody in the residual yolk of broiler chicks. The concentration of insulin-like growth factor 1 in plasma and the liver was higher (P < 0.05) in group A. Nonfasting enhanced (P < 0.05) the synthesis of protein and glycogen in the breast muscle and liver; the relative weights of the liver, pancreas, and spleen; and body weight, but retarded (P < 0.05) the synthesis of triglyceride in the liver. The results indicated that nonfasting (group A) after placement promoted the absorption of residual yolk and synthesis of protein and glycogen in the breast muscle and liver, whereas early feed deprivation promoted the synthesis of lipid in the liver. Thereby, nonfasting after placement promoted organ development and body growth of broiler chicks.


Assuntos
Estruturas Animais , Galinhas , Gema de Ovo , Privação de Alimentos , Estruturas Animais/crescimento & desenvolvimento , Animais , Peso Corporal , Galinhas/crescimento & desenvolvimento , Galinhas/metabolismo , Gema de Ovo/metabolismo , Privação de Alimentos/fisiologia , Glicogênio/biossíntese , Nutrientes/biossíntese , Biossíntese de Proteínas/fisiologia , Distribuição Aleatória
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