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1.
Chin Med J (Engl) ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31725457

RESUMO

BACKGROUND: Colorectal cancer (CRC) has become one of the major life-threatening complications in patients with inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's disease (CD). This study aimed to explore the clinical-pathologic similarities and differences in the IBD-associated CRC (IBD-CRC) between patients in China and Canada. METHODS: Data of 78 patients with IBD-CRC retrospectively retrieved from two representative medical institutions in Beijing (China) and Calgary (Canada) over the same past 13 years, including 25 (22 UC-associated and three CD-associated) from Beijing group and 53 (32 UC-associated and 21 CD-associated) from Calgary group, were compared with regards to their clinical and pathologic characteristics. RESULTS: Several known features of IBD-CRC were seen in both groups, including long duration and large extent of colitis, active inflammation background, multifocal lesions, and advanced tumor-node-metastasis stage. Beijing group showed a significantly higher percentage of UC (88.0% vs. 60.4%, P = 0.018), younger age at diagnosis of CRC (48.6 ±â€Š12.8 years vs. 61.6 ±â€Š14.7 years, P < 0.001), lower ratio of mucinous adenocarcinoma (7.1% vs. 42.4%, P = 0.001) compared with Calgary group. None of the Beijing group had concurrent primary sclerosing cholangitis, while 5.7% of Calgary group did. Surveillance colonoscopy favored the detection rate of precancerous lesions (41.4% vs.17.0%, P = 0.002). CONCLUSIONS: As compared with patients from the Calgary group, the IBD-CRC patients in Beijing group were younger, less CD-associated and had less mucinous features, otherwise they were similar in many common features.

3.
Acta Pharmacol Sin ; 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31659239

RESUMO

Osteocalcin, expressed in osteoblasts of the bone marrow, undergoes post-translational carboxylation and deposits in mineralized bone matrix. A portion of osteocalcin remains uncarboxylated (uncarboxylated osteocalcin, GluOC) that is released into blood where it functions as a hormone to regulate insulin secretion and insulin sensitivity. As insulin resistance is closely associated with metabolic syndrome, this study is aimed to elucidate how GluOC regulates glucose and lipid metabolism in KKAy mice, an animal model displaying obese, hyperglycemia, hyperinsulinemia, insulin resistance, and hepatic steatosis. GluOC (3, 30 ng/g per day, ig) was orally administered to female KKAy mice for 4 weeks. Whole-body insulin sensitivity, glucose metabolism, hepatic steatosis, dyslipidemia were examined using routine laboratory assays. We found that GluOC administration significantly enhanced insulin sensitivity in KKAy mice by activating hepatic IRß/PI3K/Akt pathway and elevated the whole-body insulin sensitivity with decreased FPI and HOMA-IR index. Furthermore, GluOC administration alleviated hyperglycemia through suppressing gluconeogenesis and promoting glycogen synthesis in KKAy mice and in cultured hepatocytes in vitro. Moreover, GluOC administration dose-dependently ameliorated dyslipidemia and attenuated hepatic steatosis in KKAy mice by inhibiting hepatic de novo lipogenesis and promoting fatty-acid ß-oxidation. These results demonstrate that GluOC effectively enhances hepatic insulin sensitivity, improves hyperglycemia and ameliorates hepatic steatosis in KKAy mice, suggesting that GluOC could be a promising drug candidate for treating metabolic syndrome.

4.
Malar J ; 18(1): 317, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31533747

RESUMO

BACKGROUND: Plasmodium falciparum apical membrane antigen-1 (PfAMA-1) is a promising candidate antigen for a blood-stage malaria vaccine. However, antigenic variation and diversity of PfAMA-1 are still major problems to design a universal malaria vaccine based on this antigen, especially against domain I (DI). Detail understanding of the PfAMA-1 gene polymorphism can provide useful information on this potential vaccine component. Here, general characteristics of genetic structure and the effect of natural selection of DIs among Bioko P. falciparum isolates were analysed. METHODS: 214 blood samples were collected from Bioko Island patients with P. falciparum malaria between 2011 and 2017. A fragment spanning DI of PfAMA-1 was amplified by nested polymerase chain reaction and sequenced. Polymorphic characteristics and the effect of natural selection were analysed using MEGA 5.0, DnaSP 6.0 and Popart programs. Genetic diversity in 576 global PfAMA-1 DIs were also analysed. Protein function prediction of new amino acid mutation sites was performed using PolyPhen-2 program. RESULTS: 131 different haplotypes of PfAMA-1 were identified in 214 Bioko Island P. falciparum isolates. Most amino acid changes identified on Bioko Island were found in C1L. 32 amino acid changes identified in PfAMA-1 sequences from Bioko Island were found in predicted RBC-binding sites, B cell epitopes or IUR regions. Overall patterns of amino acid changes of Bioko PfAMA-1 DIs were similar to those in global PfAMA-1 isolates. Differential amino acid substitution frequencies were observed for samples from different geographical regions. Eight new amino acid changes of Bioko island isolates were also identified and their three-dimensional protein structural consequences were predicted. Evidence for natural selection and recombination event were observed in global isolates. CONCLUSIONS: Patterns of nucleotide diversity and amino acid polymorphisms of Bioko Island isolates were similar to those of global PfAMA-1 DIs. Balancing natural selection across DIs might play a major role in generating genetic diversity in global isolates. Most amino acid changes in DIs occurred in predicted B-cell epitopes. Novel sites mapped on a three dimensional structure of PfAMA-1 showed that these regions were located at the corner. These results may provide significant value in the design of a malaria vaccine based on this antigen.

5.
Carbohydr Polym ; 224: 115169, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472851

RESUMO

Dialdehyde carboxymethylcellulose (DCMC) solution generally has quite low solid content, which inevitably limits its industrial application. In this work, carboxymethylcellulose sodium (Na-CMC) was pre-degraded using H2O2 followed by periodate oxidation for preparing DCMC with high solid content as practical tanning agent. Pre-degradation conditions optimization showed that H2O2 dosage most impacted the tanning effect of DCMC, and the Mw and viscosity of Na-CMC underwent remarkable reduction. FT-IR and 1H NMR illustrated that aldehyde group was successfully introduced into DCMC after periodate oxidation. Under the optimized conditions, the solid content of DCMC could be improved to around 30%. This DCMC could endow tanned leather with high shrinkage temperature and satisfactory fiber dispersion. Besides, DCMC tanned leather had comparable physical and organoleptic properties to those of leathers tanned by chrome tanning agent and commercial polyaldehyde tanning agent TWT. This suggests the prospect of DCMC with high solid content as useful tanning agent.

6.
Int J Biol Macromol ; 138: 321-333, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31295499

RESUMO

The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel. The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively. The gelling characteristic of HBC hydrogel was not influenced by composite CS and DOPA. The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate. In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h. The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis. The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.

7.
Ying Yong Sheng Tai Xue Bao ; 30(6): 1965-1973, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31257769

RESUMO

We studied the seed rain, seed pool quantity and quality dynamics, and seedling develop-ment of natural Quercus aliena var. acureserrata forests in different slopes (sunny, semi-sunny, semi-shady and shady slope) of the middle Qinling Mountains, China. The results showed that seed rain of Q. aliena var. acureserrata generally began at mid-August, peaked from mid-late September to early October, and ended in mid-early November. The dissemination process, occurrence time, and composition of seed rain in four slopes were different. The seed rain intensity of the semi-sunny slope was the highest, which was 134.13 seeds·m-2, followed by sunny slope, semi-shady slope, and shady slope. The seed rain on the sunny slope occurred first, and the duration of the whole and peak period were the longest. The seed rain on the shady slope occurred at the latest, and the duration of the whole and peak period were the shortest. The seed viability and mature seeds proportion were semi-sunny slope > sunny slope > semi-shady slope > shady slope. From the end of the seed rain to the August of the next year, the size of soil seed bank was semi-sunny slope > sunny slope > semi-shady slope > shady slope. The number of mature, immature and nibbled seeds and seed vigor of soil seed bank showed a decreasing trend with time, while the number of moldy seeds increased. The seeds in soil seed bank mainly existed in the litter layer, followed by the 0-2 cm soil layer, with few seeds in the 2-5 cm soil layer. The seedling density of Q. aliena var. acureserrata was significantly different among the four slopes. The semi-sunny slope, with the highest seedling density, was more suitable for seed germination and seedling growth of Q. aliena var. acureserrata than other slopes.


Assuntos
Quercus , Plântula , China , Banco de Sementes , Sementes , Solo/química
8.
Zhongguo Zhen Jiu ; 39(7): 787-91, 2019 Jul 12.
Artigo em Chinês | MEDLINE | ID: mdl-31286744

RESUMO

The article "Effect of acupuncture vs sham acupuncture on live births among women undergoing in vitro fertilization: a randomized clinical trial", published in JAMA in May 2018, has concluded that acupuncture does not improve the rate of live births among women undergoing IVF. Through careful study of the article, the author analyzes its reliability from acupuncture therapeutic plan and specific acupuncture operation. As a result, although the research showed no significant difference between the acupuncture group and the sham acupuncture group, it could not prove no therapeutic effect in the sham acupuncture group, so the conclusion that the acupuncture did not improve the therapeutic effect could not be drawn; the compatibility of acupoints was inconsistent with the previous protocol, and its rationality was controversial; whether the frequency and duration of acupuncture treatment could highlight the live birth rate should be further discussed. In addition, the selection of acupuncturists may be another reason for the failure of the research aim.


Assuntos
Terapia por Acupuntura , Nascimento Vivo , Feminino , Fertilização In Vitro , Humanos , Gravidez , Reprodutibilidade dos Testes
9.
Biomed Pharmacother ; 118: 109216, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31319371

RESUMO

The osseointegration process of implant is seriously impaired in type 2 diabetes mellitus (T2DM) that causes high failure rate, and insufficiency exists in current insulin therapy, creating a demand for new bone-synergistic agent. Cinaciguat, a novel type of soluble guanylate cyclase (sGC) activator, plays a vital role in glucose metabolism, inflammation control and bone regeneration. We hypothesized that the combined application of cinaciguat and insulin could reverse poor implant osseointegration in diabetes. To test this hypothesis, streptozotocin-induced diabetic rats were placed implants in the femur, and divided into five groups: control, T2DM, cinaciguat-treated T2DM (7 µg/kg), insulin-treated T2DM (12 IU/kg), cinaciguat plus insulin combination-treated T2DM (7 µg/kg and 12 IU/kg respectively), according to different treatment received. The weight and glucose levels of rats were evaluated at fixed times, and plasma level of cyclic guanosine monophosphate (cGMP) was determined before euthanasia. Three months after therapy, the femurs were isolated for pull-out test, environmental scanning electron microscope observation, microscopic computerized tomography evaluation and various histology analysis. Results revealed that diabetic rats showed the highest blood glucose level and lowest cGMP content, which led to the worst structural damage and least osseointegration. Combined treatment could attenuate the diabetes induced hyperglycemia to be normal, restore the cGMP content, protein kinase G II (PKG II) expression, phosphodiesterase-5 (PDE5) activity and ameliorate the mechanical strength, the impaired bone microarchitecture and osseointegration to the highest level. Meanwhile, monotreatment (insulin or cinaciguat) also showed restorative effect, but less. Our findings demonstrated that the cGMP/PKG II signaling pathway activated by cinaciguat mediated the favorable effects of the combined application on improving implant fixation under T2DM condition.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31265863

RESUMO

Glutamatergic dysregulation has served as one common pathophysiology of major depressive disorder (MDD) and a promising target for treatment intervention. Previous studies implicate neurotransmission via metabotropic glutamate receptors (mGluRs) and Homer1 in stress-induced anhedonia, but the mechanisms have not been well elucidated. In the present study, we used two different animal models of depression, chronic social defeat stress (CSDS) and chronic restraint stress (CRS), to investigate the expression of Homer1 isoforms and functional interaction with mGluRs. We found that chronic stress selectively upregulated the expression of Homer1b/c in the hippocampus, whereas the level of Homer1a was unchanged. Additionally, there was a significant negative correlation between the levels of Homer1-mGluR5 signaling and depressive-like behaviors. Both application of paired-pulse low-frequency stimulation (PP-LFS) and the selective group 1 mGluRs agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) significantly enhanced mGluR-dependent long-term depression (LTD) at CA3-CA1 pyramidal cell synapses in slices from susceptible mice, whereas there was no change in NMDAR-dependent LTD induced by LFS. Furthermore, these effects were associated with the internalization of surface AMPARs in hippocampal pyramidal neurons, including reduced the expression of AMPARs and amplitude of AMPARs-mediated mEPSC. Finally, we found that chronic stress activated the KR-like ER kinase-eukaryotic initiation factor 2α (PERK-eIF2α) signaling pathway, subsequently phosphorylated cAMP response element binding protein (CREB) at the S129 and reduced the BDNF level, eventually leading to the impairment of synaptic transmission and depressive-like behaviors. Therefore, our study suggests that PERK-eIF2α acts as a critical target downstream of Homer1-mGluR5 complex to mediate chronic stress-induced depressive-like behaviors, and highlights them as a potential target for the treatment of mood disorder.

11.
Int J Phytoremediation ; : 1-7, 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31321987

RESUMO

A pot experiment was conducted to study the effects of different rootstocks on the cadmium (Cd) accumulation characteristics of the post-grafting generations of Cd-hyperaccumulator Galinsoga parviflora plants. Five treatments, ungrafted and G. parviflora seedlings grafted on the rootstocks of Kalimeris indica, Senecio scandens, Conyza canadensis, and Artemisia sieversiana, were utilized. The four rootstock grafts decreased the shoot biomass of the G. parviflora post-grafting generation compared with ungrafted. The K. indica and S. scandens grafts increased the Cd concentration in shoots of the G. parviflora post-grafting generation by 15.06% and 14.40%, respectively, compared with ungrafted, while the C. canadensis and A. sieversiana grafts had no significant effects. K. indica grafts increased the amount of Cd extracted by shoots of the G. parviflora post-grafting generation by 10.59% compared with ungrafted, while the other treatments resulted in decreases. Compared with ungrafted, the different rootstocks had no significant effects on the photosynthetic pigment content of the G. parviflora post-grafting generation, and only C. canadensis grafts increased the superoxide dismutase activity level, while only K. indica grafts increased the peroxidase activity level. Therefore, the K. indica rootstock could increase the phytoremediation capability of G. parviflora post-grafted plants grown in Cd-contaminated soil.

12.
Chin Med J (Engl) ; 132(15): 1833-1842, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31268903

RESUMO

BACKGROUND: Imbalance of intestinal microbiota was closely related to colitis. Under these circumstances, regulation of enteric flora may be beneficial to the repair of inflammation. We aimed to investigate the effects of probiotics (Bifidobacterium and Lactobacillus), prebiotics and their combination on inflammation, and microflora in mice of acute colitis. METHODS: C57BL/6J mice were divided into six groups randomly (blank control group, model control group, probiotics group, synbiotics group, lactitol group and probiotics + lactitol group). Each group was given 2.5% dextran sulfate sodium drinking water for 5 days other than the blank control group. Except for the model control group, the other four groups were intervened with probiotics, synbiotics (probiotics and inulin), lactitol, and probiotics + lactitol. Mice were sacrificed after 1 week of gavage, and pathologic scores were calculated. The feces of different periods and intestinal mucosa samples were collected to analyze the differences of intestinal microbiota by 16S rRNA sequencing. Differences of two groups or multiple groups were statistically examined through unpaired Student t test and analysis of variance (ANOVA), respectively. ANOVA, Tukey, Anosim, and metastats analysis were used to compare differences of microbiota among different groups. RESULTS: After gavage for 1 week, the pathologic scores of groups with the intervention were significantly lower than those in the model control group, and the difference was statistically significant (P < 0.05). The model control group was higher in the genus of Bacteroides (relative abundance: 0.3679 vs. 0.0099, P = 0.0016) and lower in Lactobacillus (relative abundance: 0.0020 vs. 0.0122, P = 0.0188), Roseburia (relative abundance: 0.0004 vs. 0.0109, P = 0.0157), compared with the blank control group. However, the same phenomenon was not found in groups gavaged with probiotics and lactitol. Compared with model control group, mice with intervention were increased with Bifidobacterium (relative abundance: 0.0172 vs. 0.0039, P = 0.0139), Lachnospiraceae_NK4A136_group (relative abundance: 0.1139 vs. 0.0320, P = 0.0344), Lachnospiraceae_UCG-006 (relative abundance: 0.0432 vs. 0.0054, P = 0.0454), and decreased with Alistipes (relative abundance: 0.0036 vs. 0.0105, P = 0.0207) in varying degrees. The mucosal flora was more abundant than the fecal flora, and genus of Mucispirillum (relative abundance: 0.0207 vs. 0.0001, P = 0.0034) was more common in the mucosa. Lactitol group showed higher level of Akkermansia than model control group (relative abundance: 0.0138 vs. 0.0055, P = 0.0415), probiotics group (relative abundance: 0.0138 vs. 0.0022, P = 0.0041), and synbiotics group (relative abundance: 0.0138 vs. 0.0011, P = 0.0034), while probiotics + lactitol group had more abundant Akkermansia than synbiotics group (relative abundance: 0.0215 vs. 0.0013, P = 0.0315). CONCLUSIONS: Probiotics and prebiotics reduce the degree of inflammation in acute colitis mice obviously. Mice with acute colitis show reduced beneficial genera and increased harmful genera. Supplementation of probiotics and prebiotics display the advantage of increasing the proportion of helpful bacteria and regulating the balance of intestinal microbiota. Lactitol might promote the proliferation of Akkermansia.

13.
Medicine (Baltimore) ; 98(23): e15953, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31169720

RESUMO

BACKGROUND: Studies showed the controversial results about the effect of common genetic polymorphisms on the atrial fibrillation (AF) recurrence. We performed the systematic review and meta-analysis to qualify the association between common genetic polymorphisms and AF recurrence. METHODS: Articles were systematically retrieved PubMed, Web of Science, EMBASE, Wanfang, and CNKI database and 9 studies including 3204 patients were enrolled in our meta-analysis. RESULTS: Results showed that the associations were significant under rs2200733 3 genetic models (TT vs CC: odds ratio [OR] [confidence interval [CI]] = 1.336 [1.061-1.683], P = .014; CT vs CC: OR [CI] = 0.759 [0.614-0.937], P = .01; TT vs CT + CC: OR [CI] = 2.308 [1.440-3.700], P = .001). The association was significant under rs10033464 genetic model (TT vs GG: OR [CI] = 1.517 [1.165-1.976], P = .002). CONCLUSIONS: Rs13376333 on chromosome 1q21 (in KCNN3), rs7193343 and rs2106261 on chromosome 16q22 (in ZFHX3) were not associated with AF recurrence in our meta-analysis. In total, our meta-analysis found that rs2200733 and rs10033464 on chromosome 4q25 (near PITX2) were associated with the risk of AF recurrence.


Assuntos
Fibrilação Atrial/genética , Cromossomos Humanos/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único , Idoso , Feminino , Proteínas de Homeodomínio/genética , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Recidiva , Canais de Potássio Ativados por Cálcio de Condutância Baixa/genética , Fatores de Transcrição/genética
14.
Waste Manag ; 87: 407-416, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31109541

RESUMO

In this work, the leaching pattern, chemical speciation, and environmental risks of various heavy metals (Pb, Zn, Cu, Cd, Cr, and Ni) were investigated synchronously under different acid corrosion conditions through end-point pH leaching experiments. The heavy metals were present in raw, stabilized (phosphoric acid; chelating agent), and solidified (Portland cement) municipal solid waste incineration (MSWI) fly ash. The results showed that the stabilization and solidification pre-treatment could effectively decrease the leaching of most heavy metals. However, phosphoric acid stabilization and Portland cement solidification increased the solubility of Ni and Pb/Cu/Cd under low end-point pH conditions, while that of Cr and Pb increased under high end-point pH conditions. Overall, the leaching pattern of heavy metals was not affected by the addition of binders/additives. The results from speciation analysis showed that the bioavailable fractions (exchangeable and carbonate-bound) were leached out from initial raw or solidified/stabilized fly ash after distilled water leaching. However, with the decrease in end-point pH levels, the bioavailable fractions increased again due to the increase in acid corrosion on metal-bearing mineral matrixes. The risk assessment results indicated that, after exposing the raw or solidified/stabilized fly ash to highly acidic conditions, not only the high-content Pb/Zn/Cu, but also some low-content Cd posed potential risks to the environment. During the leaching process, under extremely acidic conditions, the increased environmental risks posed by Pb/Zn/Cu/Cd in residual fly ash solids were greatly ascribed to the increase in bioavailable fractions, which might result in the re-leaching of some heavy metals to the environment.


Assuntos
Incineração , Metais Pesados , Carbono , Cinza de Carvão , Corrosão , Material Particulado , Resíduos Sólidos
15.
J Biomed Mater Res A ; 107(10): 2123-2134, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31094049

RESUMO

Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration. A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells. Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release. Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization. In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).

16.
Mol Med Rep ; 19(6): 5097-5104, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31059057

RESUMO

Numerous studies have demonstrated the association between senescence and cancer. However, the molecular mechanism regulating senescence in ovarian cancer remains unknown. In the present study, the protein expression level of calbindin 1 (CALB1) in ovarian cancer was examined using western blot and immunohistochemistry. The function of CALB1 in ovarian cancer cells was examined using MTT assay, anchorage­independent growth assay and senescence assay. The molecular mechanisms underlying CALB1 function were investigated using immunoprecipitation and pull­down assays. In the present study, the expression of CALB1 was found to be increased in ovarian cancer. Overexpression of CALB1 promoted the proliferation and colony formation of ovarian cancer cells and inhibited senescence by modulating the expression levels of p21 and p27. Knockdown of CALB1 inhibited the proliferation and colony formation of ovarian cancer cells. Mechanistically, co­immunoprecipitation assays revealed that CALB1 interacts with MDM2 proto­oncogene (MDM2) and promoted the interaction between p53 and MDM2. Collectively, the present study suggested that CALB1 may act as an oncogene in ovarian cancer by inhibiting the p53 pathway.


Assuntos
Calbindina 1/metabolismo , Senescência Celular , Neoplasias Ovarianas/patologia , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Calbindina 1/antagonistas & inibidores , Calbindina 1/genética , Linhagem Celular Tumoral , Proliferação de Células , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Inibidor de Quinase Dependente de Ciclina p27/metabolismo , Feminino , Humanos , Neoplasias Ovarianas/metabolismo , Plasmídeos/genética , Plasmídeos/metabolismo , Ligação Proteica , Interferência de RNA , RNA Interferente Pequeno/metabolismo
17.
Sci Rep ; 9(1): 7738, 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31123304

RESUMO

A real-time quantitative PCR assay using a species-specific primer pair was developed to rapidly and accurately quantify Valsa mali, the causative pathogen of apple Valsa canker (AVC), in crabapple seeds, crabapple seedlings, apple twigs and apple seeds. Surveys were conducted in different regions, and crabapple or apple seeds were collected for V. mali detection by qPCR assay. Our results showed that 12.87% to 49.01% of crabapple seeds collected from different regions were positive for V. mali. The exopleura and endopleura were the two major areas of V. mali infection in crabapple seeds. The presence of V. mali infection in crabapple seeds was also confirmed by a high-throughput sequencing approach. With the growth of crabapple seedlings, the concentration of V. mali gDNA in crabapple seedlings gradually increased until eight or more leaf blades emerged. One-year-old twigs from an apple scion nursery were infected with V. mali, and only apple seeds from infected apple trees showing evident Valsa canker symptoms carried V. mali. In conclusion, this study reports that crabapple seeds and apple seeds carried V. mali as latent inoculum sources. V. mali infected not only apple tissues but also crabapple seedlings, which are the rootstocks of apple trees. This study indicated that the inoculum sources for AVC vary. Application of a novel qPCR assay can potentially improve the accuracy of early diagnosis, and is helpful to reveal the epidemic regularity of AVC.

18.
J Asian Nat Prod Res ; 21(4): 299-307, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30909734

RESUMO

Six new glycosides (1-6), together with three known ones, were isolated from the twigs and leaves of Rhododendron latoucheae. Their structures were elucidated based on the spectroscopic data, including infrared spectrometry, mass spectrometry, and nuclear magnetic resonance experiments, along with Mosher's method. In addition, all compounds were tested their antiviral (herpes simplex virus-1 and influenza A/95-359) activities.


Assuntos
Glicosídeos/isolamento & purificação , Rhododendron/química , Antivirais/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Espectroscopia de Ressonância Magnética , Folhas de Planta/química
19.
Biomed Pharmacother ; 113: 108716, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30844657

RESUMO

MicroRNA-575 (miR-575) is oncogene in many tumors. However, the role of miR-575 in the progression of gastric cancer (GC) is still unknown. The aim of this study was to identify whether miR-575 play a role in the development of GC. We obtained GC cell lines, GC tissues from 40 patients to measure the levels of miR-575 and its predicted target PTEN by using RT­PCR, immunohistochemistry or western blot analysis. MGC-803 cells were transfected with miR-575 inhibitor, and cells viability and apoptosis were measured. miR-575 aberrantly up-regulated in GC tissues and GC cell lines compared with corresponding control. In cell lines, MGC­803 expressed highest level of miR-575 among the tested cells. The level of miR-575 was correlated with the tumor size, AJCC stage and prognosis, but not with the other clinical parameters. Knockdown of miR-575 inhibited proliferation and promoted apoptotic death of MGC-803 cells both in vitro and vivo. PTEN levels (both mRNA and protein) were remarkably decreased in cancer tissues compared with the paired-adjacent tissues, and negatively correlated with miR-575 in the tissues. By using luciferase reporter assay, we found PTEN was a direct downstream target of miR-575, and negatively regulated by miR-575 via targeting its 3'UTR. Knockdown or overexpression of miR-575 in MGC-803 cells negatively regulated PTEN expression. Finally, silencing PTEN partially impaired anti-proliferative effects of miR-575 inhibitor. miRNA-575 serves a pivotal role in GC as a cancer promoter gene by targeting PTEN to regulate proliferation and apoptosis of the cancer cells.


Assuntos
Apoptose/genética , Proliferação de Células/genética , MicroRNAs/genética , PTEN Fosfo-Hidrolase/metabolismo , Neoplasias Gástricas/patologia , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Invasividade Neoplásica , PTEN Fosfo-Hidrolase/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
20.
Bioresour Technol ; 281: 84-89, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30802819

RESUMO

Alginate oligosaccharides (AOS) showed various biological activities. Traditional protocol for producing AOS was a multiple-step and high-pollution procedure. In this study, a rapid and efficient AOS producing method was developed directly from Laminaria japonica. Natural sun-dried L. japonica with a feed ratio of 1:7 (w/v) was pretreated using cellulase with a dry weight of 3%, for releasing the fermentable sugars (8.5 g/L glucose and 15.2 g/L mannitol). Then, the engineered yeast Yarrowia lipolytica strain with alginate lyase activity was grown using an algae-based medium. After fermentation for 72 h, glucose and mannitol were completely consumed, and 71.8 mM AOS was extracted from the fermentation supernatant. The degree of polymerization (DP) was ranging from 2 to 3. The recovery yield of AOS was about 91.7%. The purity of the extracted AOS was 92.6%. Overall, our work provided new insights for the development of green biotechnologies for oligosaccharide production from seaweed.


Assuntos
Fermentação , Laminaria/metabolismo , Oligossacarídeos/metabolismo , Alginatos/metabolismo , Hidrólise , Alga Marinha/metabolismo , Yarrowia/genética , Yarrowia/metabolismo
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