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1.
Sci Rep ; 9(1): 15856, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676802

RESUMO

In this paper, modulation of reflected wavefront out of the incident plane by a tunable acoustic metasurface is investigated based on the fully generalized Snell's law in the three-dimensional space. The metasurface is constructed by a square lattice of circular holes with gradient annular bumps. The phase shift is tuned by changing the volume of water filled in the holes. The acoustic wave steering out of the incident plane and the out-of-plane acoustic focusing with the oblique incidence at the subwavelength scale are demonstrated numerically by selecting suitable distributions of water depth. The numerical results show that the wavefront of the reflected wave can be manipulated over a wide frequency range; and the gradient design of the unit cells can suppress the parasitic reflection. The present work is relevant to the practical design of novel acoustic devices.

2.
J Immunother ; 42(8): 297-308, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31478975

RESUMO

On the basis of the autologous tumor-infiltrating lymphocytes (TILs) or genetically modified TILs for adoptive cell therapy have received more attention. Programmed cell death protein 1 (PD-1) expression on the T cells exert complex response during the tumor immune response. But the composition and function of PD-1T-cell subsets in TILs from human lung cancer still limited. In blood and TILs from human lung cancer patients, we confirmed that PD-1 is expressed in higher levels in CD4T-cell subsets than in CD8T-cell subsets. To further analyze the function of PD-1T cells in TILs, we observed the cytokine production in different T-cell subsets. We found that higher interferon-γ and granzyme B production in CD4/CD8PD-1T-cell subsets in TILs than in peripheral blood mononuclear cells (PBMCs); except for PD-1Tscm, higher tumor necrosis factor-α production was observed in PD-1T-cell subsets in TILs than in PBMCs; the expression level of interleukin-17 were lower in PD-1T cells in TILs than in PBMCs; and perforin expression was significantly reduced in CD4PD-1T cells subsets in TILs compared with peripheral blood. Clarify elucidating the composition and function of PD-1T-cell subsets in TILs will have great value in clinical application for evaluating the sensitivity to PD-1 blockade and selecting the promising candidate T-cell subsets in TILs for combination immunotherapy in human lung cancer.

3.
Pathol Int ; 69(6): 350-359, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31273876

RESUMO

This study aimed to investigate the association of SDH gene mutations and promoter methylation with succinate dehydrogenase-deficient gastrointestinal stromal tumors (SDH-deficient GISTs) and to further discuss the potential molecular mechanisms underlying SDHB expression loss in these tumors. First, a total of 26 patients with SDH-deficient GISTs were selected by identifying the loss of SDHB protein expression and wild-type for KIT and PDGFRa mutations. Then SDH gene mutations and promoter methylation were detected by DNA sequencing and methylation-specific polymerase chain reaction, respectively, and the clinical and pathological data of SDH-deficient GISTs patients were collected and analyzed accordingly. The results of genetic testing demonstrated that 38.46% (10/26) of these patients harbored mutations in SDHB, SDHC, and SDHD genes (3 cases with double mutations). Besides, aberrant promoter methylation of SDH genes was detected in 10 out of 26 cases (38.46%), including 8 cases in SDHA gene, 3 cases in SDHB gene, 1 case in both SDHA and SDHB genes. It is suggested that SDH gene mutations and promoter methylation may contribute to the loss of SDH protein expression in sporadic SDH-deficient GISTs. This study indicated that the genetic and epigenetic alterations of SDH genes may occur during tumor formation.

4.
Materials (Basel) ; 12(10)2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31108881

RESUMO

Submicron lenses and cylinders exhibiting excellent properties in photodetector and quantum applications have been fabricated on a diamond surface by an inductively-coupled plasma (ICP) etching technique. During ICP etching, a layer containing 500 nm diameter balls of SiO2 was employed as mask. By changing the mixing ratio of O2, Ar and CF4 during ICP etching, several submicron structures were fabricated, such as cylinders and lenses. The simulation results demonstrated that such submicron structures on a diamond's surface can greatly enhance the photon out-coupling efficiency of embedded nitrogen-vacancy center.

5.
Chin J Traumatol ; 22(1): 1-11, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30850324

RESUMO

Vacuum sealing drainage (VSD) is frequently used in abdominal surgeries. However, relevant guidelines are rare. Chinese Trauma Surgeon Association organized a committee composed of 28 experts across China in July 2017, aiming to provide an evidence-based recommendation for the application of VSD in abdominal surgeries. Eleven questions regarding the use of VSD in abdominal surgeries were addressed: (1) which type of materials should be respectively chosen for the intraperitoneal cavity, retroperitoneal cavity and superficial incisions? (2) Can VSD be preventively used for a high-risk abdominal incision with primary suture? (3) Can VSD be used in severely contaminated/infected abdominal surgical sites? (4) Can VSD be used for temporary abdominal cavity closure under some special conditions such as severe abdominal trauma, infection, liver transplantation and intra-abdominal volume increment in abdominal compartment syndrome? (5) Can VSD be used in abdominal organ inflammation, injury, or postoperative drainage? (6) Can VSD be used in the treatment of intestinal fistula and pancreatic fistula? (7) Can VSD be used in the treatment of intra-abdominal and extra-peritoneal abscess? (8) Can VSD be used in the treatment of abdominal wall wounds, wound cavity, and defects? (9) Does VSD increase the risk of bleeding? (10) Does VSD increase the risk of intestinal wall injury? (11) Does VSD increase the risk of peritoneal adhesion? Focusing on these questions, evidence-based recommendations were given accordingly. VSD was strongly recommended regarding the questions 2-4. Weak recommendations were made regarding questions 1 and 5-11. Proper use of VSD in abdominal surgeries can lower the risk of infection in abdominal incisions with primary suture, treat severely contaminated/infected surgical sites and facilitate temporary abdominal cavity closure.


Assuntos
Abdome/cirurgia , Drenagem/métodos , Medicina Baseada em Evidências , Guias de Prática Clínica como Assunto , Sociedades Médicas/organização & administração , Infecção da Ferida Cirúrgica/prevenção & controle , Traumatologia/organização & administração , Vácuo , China , Humanos
6.
Sci Rep ; 9(1): 5192, 2019 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-30914662

RESUMO

Fabrication of hydrogen-terminated diamond (H-diamond) field-effect transistor (FET) with AlOx dielectric layer has been successfully carried out. The AlOx layer was formed by auto-oxidizing 6 nm Al film in the air at room temperature, and a FET without AlOx dielectric layer has also been fabricated for comparison. For both FETs, 100 nm Al layers were deposited as the gate electrodes, respectively. The leakage current density in FET with AlOx dielectric layer was four magnitude orders lower than that without AlOx dielectric layer at VGS = -5 V, indicating that AlOx dielectric layer could effectively reduce leakage current and prevent reverse ID in ID - VDS caused by defects on diamond surface. Distinct pinch-off characteristic with p-type channel was observed in ID - VDS measurement. The threshold voltage was -0.4 V at VDS = -15 V.

7.
Mol Med Rep ; 18(6): 5663-5668, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30387846

RESUMO

Hepatic ischemia/reperfusion injury (HIRI) serves a causative role in postoperative hepatocyte death; however, the mechanisms underlying HIRI remain unclear. Mitochondrial autophagy, with apoptosis, cell cycle distribution and DNA damage repair, may be regarded as a regulatory factor post­HIRI. Parkin, a novel ubiquitin ligase, has been reported to increase mitochondrial autophagy and decrease apoptosis. However, the association between Parkin, mitochondrial autophagy and other regulatory factors in HIRI is unclear. In the present study, the effects of Parkin on HIRI were investigated, using hepatocytes and livers from male Sprague Dawley rats subjected to simulated in vivo HIRI. The results of the present study demonstrated that Parkin expression and mitochondrial autophagy were upregulated post­HIRI, leading to decreased hepatocyte death. Parkin knockdown suppresses the level of mitochondrial autophagy and promotes hepatocyte apoptosis by suppressing apoptosis regulator Bcl­2 function post­HIRI. In addition, Parkin deficiency alters cell cycle distribution and impairs DNA damage repair post­HIRI. In conclusion, Parkin facilitates mitochondrial autophagy and DNA damage repair, inhibits apoptosis, and modulates the cell cycle, leading to increased hepatocyte survival, demonstrating that Parkin may act as a protective regulatory factor post HIRI.


Assuntos
Apoptose/genética , Ciclo Celular/genética , Dano ao DNA/genética , /genética , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/metabolismo , Ubiquitina-Proteína Ligases/deficiência , Animais , Modelos Animais de Doenças , Regulação da Expressão Gênica , Técnicas de Silenciamento de Genes , Hepatócitos/metabolismo , Hepatopatias/etiologia , Hepatopatias/metabolismo , Hepatopatias/patologia , Masculino , Mitocôndrias/genética , Mitocôndrias/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Traumatismo por Reperfusão/patologia
8.
Sci Rep ; 7(1): 12157, 2017 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-28939907

RESUMO

Investigation of ohmic contact between iridium (Ir) film and hydrogen-terminated single crystal diamond has been carried out with annealing temperature from 300 to 600 °C in argon (Ar) and hydrogen ambient. Electrodes were deposited on hydrogen-terminated single crystal diamond by electron beam evaporation technique, and specific contact resistivity has been measured by transmission line model. The interface between Ir film and hydrogen-terminated single crystal diamond was characterized by transmission electron microscopy and energy dispersive X-ray spectroscopy. Theoretical calculation value of barrier height between Ir film and hydrogen-terminated single crystal diamond was around -1.1 eV. All results indicate that an excellent ohmic contact could be formed between Ir film and hydrogen-terminated single diamond.

9.
Cryobiology ; 75: 100-105, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-28093198

RESUMO

BACKGROUND: Mild hypothermia has been well known as an effective way to reduce ischemia reperfusion injury (IRI), while the mechanisms are still unclear. More and more evidences have indicated that miRNAs should been involved in the regulation of IRI and expecially some miRNAs have shown temp-responsiveness for temperature variation. Therefore, the role of miR-122 in mild hypothermia pretreatment after IRI was investigated. METHODS: We established a LO2 cell anoxia-reoxygenation injury model to simulate liver IRI. Five groups of differently pretreated L02 cells were studied. ALT, AST and LDH as well as cell viability were measured. Flow cytometric analysis was used to evaluate the apoptosis. The expression of miR-122 was quantified by qRT-PCR. Insulin-like growth factor 1 receptor (IGF-1R), protein kinase B (p-AKT), AKT, forkhead box O3a (p-FOXO3a) and Caspase3 were examined using western blot analysis. RESULTS: We found that mild hypothermia pretreatment could reduce the hepatocellular injury and induce a significant down-regulation in miR-122 expression after IRI. However, those effects of protection were attenuated by overexpressed miR-122 blockade. We further demonstrated that down-regulation of miR-122 promoted IGF-1R translation and AKT activity, suppressed FOXO3a activity and Caspase3 expression after mild hypothermia pretreatment, which was abrogated by miR-122 mimic. CONCLUSION: Our data clearly demonstrate that mild hypothermia pretreatment can down-regulate miR-122 to protect hepatocytes against IRI through activation IGF-1R/AKT signaling pathway and inhibit cells apoptosis.


Assuntos
Hepatócitos/metabolismo , Hipotermia Induzida , MicroRNAs/biossíntese , Receptor IGF Tipo 1/biossíntese , Traumatismo por Reperfusão/metabolismo , Apoptose/efeitos dos fármacos , Regulação para Baixo , Humanos , Hipotermia Induzida/métodos , Transdução de Sinais/efeitos dos fármacos
10.
Bioorg Med Chem Lett ; 27(3): 562-566, 2017 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-28003138

RESUMO

(-)-Dehydroxymethylepoxyquinomicin ((-)-DHMEQ, 1) is a specific inhibitor of NF-κB. It binds to SH group in the specific cysteine residue of NF-κB components with its epoxide moiety to inhibit DNA binding. In the present research, we have designed and synthesized an epoxide-free analog called (S)-ß-salicyloylamino-α-exo-methylene-Æ´-butyrolactone (SEMBL, 3). SEMBL inhibited DNA binding of NF-κB component p65 in vitro. It inhibited LPS-induced NF-κB activation, iNOS expression, and inflammatory cytokine secretions. It also inhibited NF-κB and cellular invasion in ovarian carcinoma ES-2 cells. Moreover, its stability in aqueous solution was greatly enhanced compared with (-)-DHMEQ. Thus, SEMBL has a potential to be a candidate for a new anti-inflammatory and anticancer agent.


Assuntos
4-Butirolactona/farmacologia , Anti-Inflamatórios não Esteroides/farmacologia , Desenho de Drogas , NF-kappa B/antagonistas & inibidores , Salicilamidas/farmacologia , 4-Butirolactona/síntese química , 4-Butirolactona/química , Animais , Anti-Inflamatórios não Esteroides/síntese química , Anti-Inflamatórios não Esteroides/química , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Humanos , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Estrutura Molecular , NF-kappa B/metabolismo , Células RAW 264.7 , Salicilamidas/síntese química , Salicilamidas/química , Relação Estrutura-Atividade
11.
Ying Yong Sheng Tai Xue Bao ; 28(11): 3553-3561, 2017 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-29692098

RESUMO

The 30-year-old biological soil crusts dominated by mosses (hereafter moss crusts) and corresponding uncrusted soil (hereafter no crust) were sampled on loess soil and aeolian soil at 0-12 cm depth on the Loess Plateau of China. Afterwards, the hydrolase activities of the samples were measured, and their correlations with soil physicochemical properties were analyzed. Compared with no crust, the activities of urease, alkaline phosphatase, invertase, and protease of the moss crusts were 2.4, 7.6, 20.7, 2.4 times higher on loess soil, respectively; while they were 3.5, 22.2, 22.3, 2.0 times higher on aeolian soil, respectively. Compared with no crust, the soil water content of the moss crusts was decreased by 6.5% and 0.8% on loess soil and aeolian soil, and the soil temperature of the moss crusts was decreased by 0.8 and 2.5 ℃, respectively; the contents of soil organic matter, alkali-hydrolyzable nitrogen, and available phosphorus of the moss crusts were 2.5, 2.9 and 3.6 times higher on the loess soil, and they were 3.6, 3.0 and 6.6 times higher on the aeolian soil, respectively. The soil hydrolase activities were positively correlated with the soil nutrient content and negatively correlated with the soil water content. Soil temperature was positively correlated with the urease activity and negatively correlated with the protease activity. The moss crusts significantly improved soil nutrients and fertility through increasing soil enzyme activities on the Loess Plateau of China, and these effects were reasonably attributed to the moderate soil water content and temperature regulated by the moss crusts.


Assuntos
Microbiologia do Solo , Vento , China , Cianobactérias , Ecossistema , Solo , Água
12.
Onco Targets Ther ; 9: 6935-6943, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27895490

RESUMO

BACKGROUND: Paxillin is implicated in tumorigenesis, progression and aggressive phenotypes of various malignancies, highlighting its functions in cellular adhesion, migration and survival. However, the roles of paxillin in human gliomas remain unclear. The aim of this study was to evaluate the clinical implication of paxillin expression in patients with gliomas and its biological function in glioma cells. PATIENTS AND METHODS: Expression levels of paxillin gene and protein, respectively, were detected by quantitative real-time reverse transcription polymerase chain reaction, Western blot and immunohistochemistry analyses in 120 pairs of glioma and matched nontumorous brain tissues. The associations between paxillin expression and various histopathological features of glioma patients were also statistically evaluated. Then, the functions of paxillin in cell migration and invasion of glioma cell lines were determined by transwell assays in vitro. RESULTS: The expression levels of both paxillin gene and protein in glioma tissues were markedly higher than those in matched nontumorous brain tissues. Notably, paxillin overexpression was significantly associated with the grade of malignancy (P<0.05). Moreover, the enforced expression of paxillin promoted the migration and invasion of glioma cells, while the loss of paxillin expression efficiently suppressed cell migration and invasion of glioma cell lines. CONCLUSION: Our data suggest that paxillin may function as an oncogene and its overexpression may be closely correlated with tumor progression of human gliomas by modulating tumor cell motility, implying the potential of paxillin as a new therapeutic target for glioma intervention.

13.
Neurosci Lett ; 630: 233-240, 2016 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-27497919

RESUMO

The study investigated the roles and mechanisms of Salvianolic acid B (Sal B) on permeability of rat brain microvascular endothelial cells (RBMECs) exposed to high glucose. The results demonstrated that Sal B greatly up-regulated the expression of tight junction (TJ) proteins and decreased the permeability of RBMECs compared with the control group. And the increase of reactive oxidative species (ROS) production, the upregulation of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) protein induced by high glucose were antagonized by Sal B. In addition, a great decrease of microRNA-200b (miR-200b) was observed in the RBMECs under high-glucose condition, which was significantly increased by Sal B pretreatment. And overexpression of miR-200b markedly attenuated the RBMECs permeability and inhibited the expression of VEGF protein by targeting with 3'-UTR of its mRNA. This led to the conclusion that Sal B-mediated improvement of blood-brain barrier dysfunction induced by high-glucose is related to the ROS/HIF-1α/VEGF and miR-200b/VEGF signaling pathways.


Assuntos
Benzofuranos/administração & dosagem , Barreira Hematoencefálica/efeitos dos fármacos , Barreira Hematoencefálica/metabolismo , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , MicroRNAs/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Células Cultivadas , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Glucose/administração & dosagem , Microvasos/efeitos dos fármacos , Microvasos/metabolismo , Ocludina/metabolismo , RNA Mensageiro/metabolismo , Ratos , Transdução de Sinais/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacos , Proteína da Zônula de Oclusão-1/metabolismo
14.
Int J Biochem Cell Biol ; 78: 297-306, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27477314

RESUMO

During the fermentation process, Clostridium acetobutylicum cells are often inhibited by the accumulated butanol. However, the mechanism underlying response of C. acetobutylicum to butanol stress remains poorly understood. This study was performed to clarify such mechanism through investigating the butanol stress-associated intracellular biochemical changes at acidogenesis phase (i.e., middle exponential phase) and solventogenesis phase (i.e., early stationary phase) by a gas chromatography-mass spectrometry-based metabolomics strategy. With the aid of partial least-squares-discriminant analysis, a pairwise discrimination between control group and butanol-treated groups was revealed, and 27 metabolites with variable importance in the projection value greater than 1 were identified. Under butanol stress, the glycolysis might be inhibited while TCA cycle might be promoted. Moreover, changes of lipids and fatty acids compositions, amino acid metabolism and osmoregulator concentrations might be the key factors involved in C. acetobutylicum metabolic response to butanol stress. It was suggested that C. acetobutylicum cells might change the levels of long acyl chain saturated fatty acids and branched-chain amino acids to maintain the integrity of cell membrane through adjusting membrane fluidity under butanol stress. The increased level of glycerol was considered to be correlated with osmoregulation and regulating redox balance. In addition, increased levels of some amino acids (i.e., threonine, glycine, alanine, phenylalanine, tyrosine, tryptophan, aspartate and glutamate) might also confer butanol tolerance to C. acetobutylicum. These results highlighted our knowledge about the response or adaptation of C. acetobutylicum to butanol stress, and would contribute to the construction of feasible butanologenic strains with higher butanol tolerance.


Assuntos
Biocombustíveis/microbiologia , Butanóis/metabolismo , Butanóis/farmacologia , Clostridium acetobutylicum/citologia , Clostridium acetobutylicum/metabolismo , Fermentação , Espaço Intracelular/efeitos dos fármacos , Espaço Intracelular/metabolismo , Clostridium acetobutylicum/efeitos dos fármacos , Clostridium acetobutylicum/fisiologia , Relação Dose-Resposta a Droga , Fermentação/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos
15.
J Acoust Soc Am ; 139(6): 3311, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27369156

RESUMO

In this paper, bandgap and dynamic effective properties of two-dimensional elastic metamaterials with a chiral comb-like interlayer are studied by using the finite element method. The effects of the geometrical parameters of the chiral comb-like interlayer on the band edges are investigated and discussed. Combined with the analysis of the vibration modes at the band edges, equivalent spring-mass/pendulum models are developed to investigate the mechanisms of the bandgap generation. The analytically predicted results of the band edges, including the frequency where the double negative properties appear, and the numerical ones are generally in good agreement. The research findings in this paper have relevant engineering applications of the elastic metamaterials in the low frequency range.

16.
Brain Res ; 1636: 81-92, 2016 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-26835555

RESUMO

Previous studies have shown that modulation of the receptor-mediated endocannabinoid system during ischemia injury can induce potent neuroprotective effects. However, little is known about whether cannabinoid-2 (CB2) receptor agonist would produce a protective effect on blood-spinal cord barrier (BSCB) during ischemia. Using an in vivo transient spinal cord ischemia model in rats, JWH-015 (1mg/kg, i.p.), a CB2 receptor selective agonist, or vehicles were injected 20 min before ischemia. The effects of JWH-015 on BSCB permeability, the major structural protein for the formation of caveolae, caveolin-1 (cav-1), tight junction (TJ) protein Occludin and zona occludens protein-1 (ZO-1) were examined at day 1, day 3 and day 7 of reperfusion after transient spinal cord ischemia in rats. Here we demonstrated that JWH-015 significantly down-regulated the expression of cav-1, up-regulated the expression of TJ proteins, and then decreased the permeability of BSCB compared with control group. In addition, using an in vitro BBB model, oxygen glucose deprivation (OGD) was applied to simulate spinal cord ischemia in vitro in Human brain microvascular endothelial cells (HBMECs). JWH-015 greatly increased the transepithelial electrical resistance (TEER) and changed the distribution of ZO-1 and Occludin. Moreover, JWH-015 induced the expression of p-PKB and p-FoxO1 protein and decreased the expression of cav-1, which were greatly reversed by ROS inhibitor or PI3K inhibitor. Taken together, all of these results suggested that JWH-015 might regulate the BSCB permeability and this effect could be related to paracellular and transcellular pathway. And pharmacological CB2R ligands offer a new strategy for BSCB protection during ischemic injury.


Assuntos
Barreira Hematoencefálica/efeitos dos fármacos , Indóis/farmacologia , Receptor CB2 de Canabinoide/agonistas , Traumatismo por Reperfusão/patologia , Junções Íntimas/efeitos dos fármacos , Animais , Permeabilidade Capilar/efeitos dos fármacos , Modelos Animais de Doenças , Impedância Elétrica , Azul Evans/farmacocinética , Glucose/deficiência , Peroxidase do Rábano Silvestre/farmacocinética , Hipóxia/patologia , Técnicas In Vitro , Masculino , Ocludina/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Ratos Wistar , Medula Espinal , Proteína da Zônula de Oclusão-1/metabolismo
17.
World J Microbiol Biotechnol ; 31(12): 2003-13, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26385547

RESUMO

Bacteriophage can be used as a potential alternative agent for controlling Lactobacillus plantarum contamination during bioethanol production. However, how Saccharomyces cerevisiae respond against contaminative L. plantarum or added bacteriophage remains to be fully understood. In this study, gas chromatography-mass spectrometry and a multivariate analysis were employed to investigate the intracellular biochemical changes in S. cerevisiae cells that were elicited by L. plantarum contamination or bacteriophage treatment. The intracellular metabolite profiles originating from different groups were unique and could be distinguished with the aid of principal component analysis. Moreover, partial least-squares-discriminant analysis revealed a group classification and pairwise discrimination, and 13 differential metabolites with variable importance in the projection value greater than 1 were identified. The metabolic relevance of these compounds in the response of S. cerevisiae to L. plantarum contamination or bacteriophage treatment was discussed. Besides generating lactic acid and competing for nutrients or living space, L. plantarum contamination might also inhibit the growth of S. cerevisiae through regulating the glycolysis in S. cerevisiae. Moreover, increased concentrations of monounsaturated fatty acids secondary to bacteriophage treatment might lead to more membrane fluidity and promote the cell viability of S. cerevisiae.


Assuntos
Bacteriófagos/metabolismo , Cromatografia Gasosa-Espectrometria de Massas/métodos , Lactobacillus plantarum/metabolismo , Metabolômica , Saccharomyces cerevisiae/metabolismo , Técnicas de Cocultura , Etanol/metabolismo , Fermentação , Glicólise , Ácido Láctico/metabolismo , Análise Multivariada
18.
Int J Biol Macromol ; 81: 387-95, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26277748

RESUMO

The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product. Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized. The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0. AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of ß-glycoside linkages at the 1200-1000cm(-1) peaks. However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH. The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Basidiomycota/química , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Antioxidantes/isolamento & purificação , Cromatografia Gasosa , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Polissacarídeos Fúngicos/isolamento & purificação , Hidrólise , Radical Hidroxila/antagonistas & inibidores , Oxirredução/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier
19.
J Neurol Sci ; 346(1-2): 51-9, 2014 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-25129208

RESUMO

Previous studies have shown that curcumin (Cur) can produce potent neuroprotective effects against damage due to spinal cord injury (SCI). However, whether Cur can preserve the function of the blood-spinal cord barrier (BSCB) is unclear. The present study was performed to investigate the mechanism underlying BSCB permeability changes, which were induced by treatment with Cur (75, 150, and 300 mg/kg, i.p.) after compressive SCI in rats. BSCB permeability was evaluated by Evans blue leakage. Motor recovery of rats with SCI was assessed using the Basso, Beattie, and Bresnahan scoring system every day until the 21st days post-injury. The protein levels of heme oxygenase-1 (HO-1), tight junction protein, and inflammatory factors were analyzed by western blots. The expression of the inflammatory factors tumor necrosis factor-α (TNF-α) and nuclear factor-kappaB (NF-κB) mRNA was determined with reverse transcription-polymerase chain reactions. Treatment with Cur (150 and 300 mg/kg) significantly reduced Evans blue leakage into the spinal cord tissue at 24h after SCI. Cur (150 mg/kg) significantly increased HO-1 protein expression. The levels of TNF-α and NF-κB mRNA and protein greatly increased at 24h after SCI, and this increase was significantly attenuated by Cur treatment. ZO-1 and occludin expression was upregulated by Cur (150 mg/kg) treatment after SCI, and this effect was blocked by the HO-1 inhibitor zinc protoporphyrin. Long-term effects of Cur on motor recovery after SCI were observed. Our results indicated that Cur can improve motor function after SCI, which could correlate with improvements in BSCB integrity.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Permeabilidade Capilar/efeitos dos fármacos , Curcumina/uso terapêutico , Compressão da Medula Espinal/tratamento farmacológico , Compressão da Medula Espinal/patologia , Junções Íntimas/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Método Duplo-Cego , Azul Evans , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Transtornos dos Movimentos/tratamento farmacológico , Transtornos dos Movimentos/etiologia , NF-kappa B/genética , NF-kappa B/metabolismo , Ocludina/genética , Ocludina/metabolismo , Protoporfirinas/uso terapêutico , Ratos , Ratos Sprague-Dawley , Medula Espinal/patologia , Compressão da Medula Espinal/complicações , Junções Íntimas/patologia , Fatores de Tempo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo , Proteína da Zônula de Oclusão-1/genética , Proteína da Zônula de Oclusão-1/metabolismo
20.
Cytotechnology ; 66(2): 309-16, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23896701

RESUMO

Spermatogonial stem cells (SSCs) are the only type of cells that transmit genes to the subsequent generations. The proliferation, cultivation and identification of SSCs in vitro are critical to understanding of male infertility, genetic resources and conservation of endangered species. To investigate the effects of glial cell-derived neurotrophic factor (GDNF) and leukemia inhibitory factor (LIF) on the proliferation of mouse SSCs in vitro, supplement of GDNF and/or LIF were designed to culture SSCs. The testes of 6-8 d mouse were harvested and digested by two-step enzyme digestion method. The SSCs and Sertoli cells were separated by differential plating. Then the SSCs were identified by alkaline phosphatase staining, RT-PCR and indirect immunofluorescence cell analysis. The cellular proliferation capacity was measured by methyl thiazolyl tetrazolium assay. The results showed that addition of 20 and 40 ng/ml of GDNF could strongly promote growth of mouse SSCs (p < 0.05). There was no significant difference between LIF treatment groups and the control group in promoting proliferation of the mouse SSCs (p > 0.05). However, the combination of 20 ng/ml GDNF and 1,000 U/ml LIF could significantly enhance the invitro proliferation of mouse SSCs (p < 0.05), and the OD490 value was 0.696 at day 5 of culture when the density of SSCs was 5-10 × 10(4) cells/ml.

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