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1.
Biochem Biophys Res Commun ; 516(3): 991-998, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31277941

RESUMO

Spinal cord injury (SCI) is terrible damage leading to the deficiencies and results in infinite inconvenience to sufferers. The effective treatment for SCI still meets a larger number of problems. Herein, the underlying molecular mechanism and novel therapy of SCI are urgently to investigate. Arachidonate 12-lipoxygenase (ALOX12) is widely expressed in various cell types and plays important role in modulating different cellular processes, such as platelet aggregation, cell migration and cancer cell proliferation. Nevertheless, the effects of ALOX12 on SCI are unclear. In the study, SCI model was established in wild type (WT) mice and ALOX12 knockout mice. First, ALOX12 expression was up-regulated in spinal cord tissues of WT mice after SCI. ALOX12-knockout mice exhibited improved behavior after SCI operation. Glial activation triggered by SCI was also alleviated in mice with the loss of ALOX12, as evidenced by the down-regulated expression of glial fibrillary acidic protein (GFAP) and Iba-1 in spinal cord samples. Further, SCI-induced inflammation was markedly prevented in ALOX12-knockout mice through blocking inhibitor of NF-κB α (IκBα)/nuclear factor-κB (NF-κB) pathway signaling. Additionally, reducing ALOX12 expression attenuated apoptosis in spinal cord tissues of SCI mice by decreasing Cyto-c, cleaved Caspase-3 and poly (ADP-ribose) polymerases (PARP) expression. The protective role of ALOX12-decrease against SCI was verified in LPS-incubated glial cells through repressing inflammatory response and apoptotic formation. Moreover, transgenic mice with ALOX12 over-expression showed accelerated SCI, associated with intensified inflammation and apoptosis. Based on these results, strategies for inhibiting ALOX12 could be used to prevent SCI development by repressing inflammation and apoptosis.

2.
Medicine (Baltimore) ; 98(20): e15448, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31096440

RESUMO

BACKGROUND: The single nucleotide polymorphism (SNP) rs2476601 of the protein tyrosine phosphatase, nonreceptor type 22 (PTPN22) gene has been presented to implicate in the pathogenesis of alopecia areata (AA) in a few association investigations with limited sample size and inconsistent conclusions. METHODS: The aim of the current meta-analysis was to assess and synthesize the presently available data on the connection between rs2476601 and AA vulnerability. Six electronic databases, including EMBASE, PubMed, Web of Science, the Cochrane Library, Wanfang data, and the China National Knowledge Infrastructure database (CNKI), were systematically retrieved for relevant observational studies published previous to November 2018. Total odds ratios (ORs) and corresponding 95% confidence intervals (95% CIs) were analyzed to evaluate the correlation between PTPN22 polymorphism and AA. Risk of bias was estimated according to the Newcastle-Ottawa Scale (NOS). Sensitivity analyses were carried out using the RevMan 5.3 software. RESULTS: In general, 5 case-control studies including 1129 AA patients and 1702 healthy control individuals were obtained for this meta-analysis. The pooled results suggested that rs2476601 SNP was significantly associated with AA susceptibility under allelic model (C vs T, OR = 0.77, 95% CI, 0.64-0.92, P = .003) and recessive model (CC vs CT + TT, OR = 0.73, 95% CI, 0.60-0.88, P = .001). CONCLUSION: On the basis of the results of the current research, the rs2476601 polymorphism of PTPN22 gene is significantly correlated with AA susceptibility. The C-allele and CC-genotype carriers at this locus have a lower risk of AA.


Assuntos
Alopecia em Áreas/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Proteína Tirosina Fosfatase não Receptora Tipo 22/genética , Alelos , Alopecia em Áreas/etnologia , Alopecia em Áreas/fisiopatologia , Estudos de Casos e Controles , Feminino , Genótipo , Heterozigoto , Humanos , Masculino , Estudos Observacionais como Assunto , Risco , Índice de Gravidade de Doença
3.
Ying Yong Sheng Tai Xue Bao ; 30(5): 1667-1678, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31107024

RESUMO

Rapidly and accurately predicting leaf area (LA) and leaf dry mass (LDM) are essential for exploring the response of plant traits to climate change. Empirical models suitable for predicting LA and LDM of a single leaf for various broadleaved tree species at the regional scale have not been proposed. We selected six broadleaved tree species in four mixed broadleaved-Korean pine (Pinus koraiensis) forests in northeastern China, including Betula platyphylla, Tilia amurensis, Populus davidiana, Betula costata, Fraxinus mandshurica and Ulmus laciniata, and measured leaf length, leaf width, leaf thickness, LA and LDM at different canopy layers (top, middle, and low). Using the median of leaf length and width ratio as the classification criterion, the six species were sorted into two groups. We tested whether different canopy layers for each group of broadleaved tree species had significant impacts on the empirical model for predicting LA and LDM. We constructed empirical models suitable for predicting LA and LDM of a single leaf at different canopy layers at the regional scale, and verified their forecast accuracy, and further evaluated their applicability for predicting LA and LDM of same broadleaved tree species in other regions. These results showed that the LA of a single leaf increased significantly with the decreases of canopy height for the six tree species, while the LDM of some broadleaved tree species showed a downward trend. The canopy height had significant impacts on constructing the empirical model for LA and LDM. The average forecast accuracy of empirical model was 95% and 83% for LA and LDM of a single leaf across canopy layers for two groups of broadleaved tree species, respectively. The average forecast accuracy was 94% and 80% for predicting LA and LDM of corresponding broadleaved tree species in other regions, respectively, indicating that the empirical models constructed in this study had a universal applicability in Northeast China.


Assuntos
Monitoramento Ambiental/métodos , Florestas , Pinus , Árvores , China , Folhas de Planta
4.
Kaohsiung J Med Sci ; 35(3): 133-138, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30887719

RESUMO

Hepatitis B virus (HBV) infection is one of major causes of various kinds of liver diseases. Among the four open reading frames (ORFs) of the HBV genome, the X region of HBV encodes HBx protein, which plays an important regulatory role in HBV infection. NF-κB and high-mobility group protein box1 (HMGB1) are potentially able to enhance the scavenging activity of host cells against foreign microorganisms. The present study focuses on the effect of HBx on the expression of HMGB1 in vitro. First, the lentiviral vector was used to induce the overexpression of HBx protein in LO2 cells (a normal hepatocyte cell line). Then, NF-κB activity, HMGB1 expression and the production of ROS were detected by Western blot and DCFH-DA (ROS detector) staining. Afterward, rotenone and LPS, which are activors of ROS and NF-κB, respectively, were used to stimulate HBx-overexpressing cells. Then, the expression differentiation of HMGB1 and ROS or the activity alternation of NF-κB was detected again. HBx inhibited the activity of NF-κB, inhibited the expression of HMGB1 and inhibited the production of ROS. The stimulation study with retenone or LPS suggested that there were mutual regulations between NF-κB and HMGB1. HBx inhibits the expression of HMGB1 and the generation of ROS via the NF-κB signaling pathway.


Assuntos
Regulação da Expressão Gênica , Proteína HMGB1/genética , NF-kappa B/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais , Transativadores/metabolismo , Linhagem Celular , Citocinas/metabolismo , Proteína HMGB1/metabolismo , Humanos , Mediadores da Inflamação/metabolismo
5.
Biosci Rep ; 2018 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-30420491

RESUMO

Diabetic nephropathy (DN) is one of the most devastating complications of diabetes mellitus. Carbohydrate response element binding protein (ChREBP) is a basic helix-loop-helix leucine zipper transcription factor that primarily mediates glucose homeostasis in the body. The present study investigated the role of ChREBP in the pathogenesis of DN. The expression of ChREBP was detected in patients with type 2 diabetes mellitus (T2DM), diabetic mice and mesangial cells. ELISA was used to measure cytokine production in mesangial cells. Flow cytometry analysis was performed to detect the apoptosis of mesangial cells in the presence of high glucose. The expression levels of ChREBP and several cytokines (TNFα, IL-1ß, and IL-6) were up-regulated in T2DM patients. The mRNA and protein levels of ChREBP were also significantly elevated in the kidneys of diabetic mice. Moreover, glucose treatment promoted mRNA levels of TNF-α, IL-1ß and IL-6 in mesangial cells. Glucose stimulation induced significant apoptosis of SV40 MES 13 cells. In addition, transfection with ChREBP siRNA significantly inhibited ChREBP expression. Consequently, the inflammatory responses and apoptosis were inhibited in SV40 MES 13 cells. These results demonstrated that ChREBP could mediate the inflammatory response and apoptosis of mesangial cells, suggesting that ChREBP may be involved in the pathogenesis of DN.

6.
Pharmazie ; 73(9): 526-532, 2018 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-30223936

RESUMO

Artesunate is a semi-synthetic derivative of a Chinese herb named Artemisia annua L. that is commonly used as an antimalarial agent in the history of traditional Chinese medicine. Many studies have reported artesunate possesses anti-inflammatory and immunoregulation properties. The present study was conducted to explore whether artesunate was effective in experimental autoimmune myasthenia gravis (EAMG) in Lewis rats. Our data showed that artesunate could improve the clinical symptoms and suppress the development of EAMG. Artesunate exerted its immunomodulatory effects by inhibiting lymphocyte proliferation and the expression of costimulatory molecules CD86, modulating Th1/Th2 cytokine expression levels, and enhancing the level of Treg cells. The final result of administration of artesunate was the decreased synthesis of anti-R97-116 IgG, IgG2a, and IgG2b antibodies. The treatment effect of artesunate was more obvious at dose of 10 mg/kg. These date suggest that artesunate might be a potential drug for the treatment of human myasthenia gravis (MG).

7.
J Endourol ; 32(10): 955-960, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30062905

RESUMO

OBJECTIVE: To evaluate the modifications and feasibility of radical videoscopic inguinal lymphadenectomy (VIL). PATIENTS AND METHODS: From January 2010 to December 2017, more than 200 patients who have underwent bilateral radical inguinal lymphadenectomy for penile cancer in Sun Yat-Sen University Cancer Center. And there were 33 patients who received radical VIL and 174 patients who received open inguinal lymphadenectomy (OIL). According to similar factors of age, body mass index, T stage, and N stage, two matched groups were created with a rate of 1:2, one group received VIL, and another group received OIL. The numbers of harvested lymph nodes, operating times, and complications were compared between the two groups. Descriptive statistical analyses, t tests, chi-square tests, and rank sum tests were performed. RESULTS: In total, 93 patients were selected, including 31 patients who underwent bilateral VIL and 62 who underwent OIL. The numbers of harvested lymph nodes did not differ significantly (p = 0.983), the operating time was longer for the VIL than the open lymphadenectomy (p < 0.01), and the morbidity was lower among the VIL than the open lymphadenectomy. CONCLUSIONS: Modified radical VIL is feasible, practical, and results in reduced morbidity. The dissecting field and the defined plane were critical to these modifications.

8.
Huan Jing Ke Xue ; 39(2): 684-690, 2018 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964831

RESUMO

Spatio-temporal distribution of PM2.5 and variations in the relationship between PM2.5 and other pollutants are the main components of PM2.5spatio-temporal statistical analysis. Existing methods directly analyze spatio-temporal distribution based on monitoring data; thus, it is difficult to effectively reveal the aggregation structure of PM2.5 concentrations. Geographically weighted regression, commonly used to model the relationships between PM2.5 and other pollutants, cannot accurately describe the spatio-temporal variability of dependency. In this study, the clustering structure of PM2.5 concentrations in Beijing was identified using the spatial clustering algorithm and the seasonal distribution characteristics of PM2.5 were analyzed based on the clustering results. The relationship between PM2.5 and PM10 was modeled by geographically and temporally weighted regression and the spatio-temporal variability of dependency was analyzed according to the regression results. The results showed that PM2.5 pollution levels and spatial variability were lower in spring and summer than those in autumn and winter and the concentration of PM2.5 in each season was characterized by low spatial distribution in the north and high spatial distribution in the south. Geographically and temporally weighted regression showed better performance; the correlations between PM2.5 and PM10 in spring and summer are weaker than those in autumn and winter and the correlation between PM2.5 and PM10 in the northwest is stronger than that in the southeast in each season.

9.
Ying Yong Sheng Tai Xue Bao ; 29(6): 1745-1752, 2018 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-29974681

RESUMO

Rapid and accurate measurement of leaf area (LA) and leaf dry mass (LM) is one of the basic requirement for leaf geometry and plant functional studies. It is important not only for studying leaf morphology and biomass estimation, but also for understanding the response mechanism of vegetation to climate change. In this study, we took two temperate deciduous broad-leaved tree species, Ulmus laciniata and Acer tegmentosum, as the study objects, constructed empirical models between LA or LM and leaf structure parameters (e.g., leaf length, L; leaf width, W) to reveal the interspecific variability in the selection of empirical model formats (linear or non-linear) and independent variables. We evaluated the forecast accuracy of these empirical models in predicting LA and LM for each species. The results showed that the optimal empirical models for predicting LA were LA=0.614L1.468W0.464 and LA=0.865(LW)0.933, and for predicting LM were LM=0.003L1.537W0.365 and LM=0.001L2.318 for U. laciniata and A. tegmentosum, respectively. The forecast accuracies of empirical models in predicting LA were 88% and 96%, and for LM were 73% and 83% for U. laciniata and A. tegmentosum, respectively. In addition, based on the empirical models for predicting LA and LM, the specific leaf area also could be indirectly measured under non-destructive conditions, with the forecast accuracies being 83% and 90% for U. laciniata and A. tegmentosum, respectively. These results provide a technical support for the efficient measurement of leaf traits and their dynamics.


Assuntos
Mudança Climática , Folhas de Planta/crescimento & desenvolvimento , Acer , China , Árvores
10.
Braz J Med Biol Res ; 51(7): e6201, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29791586

RESUMO

This study aimed to investigate the role of hypoxia-inducible factor-2α (HIF-2α) in the expression of tight junction proteins and permeability alterations in rat glomerular endothelial cells (rGENCs) under hypoxia conditions. The expression level of HIF-2α and tight junction proteins (occludin and ZO-1) in rGENCs were examined following 5% oxygen density exposure at different treatment times. HIF-2α lentivirus transfection was used to knockdown HIF-2α expression. Cells were divided into four groups: 1) control group (rGENCs were cultured under normal oxygen conditions), 2) hypoxia group (rGENCs were cultured under hypoxic conditions), 3) negative control group (rGENCs were infected with HIF-2α lentivirus negative control vectors and cultured under hypoxic conditions), and 4) Len group (rGENCs were transfected with HIF-2α lentivirus and cultured under hypoxic conditions). The hypoxia, negative control, and Len groups were kept in a hypoxic chamber (5% O2, 5% CO2, and 90% N2) for 24 h and the total content of occludin and ZO-1, and the permeability of rGENCs were assessed. With increasing hypoxia time, the expression of HIF-2α gradually increased, while the expression of occludin decreased, with a significant difference between groups. ZO-1 expression gradually decreased under hypoxia conditions, but the difference between the 24 and 48 h groups was not significant. The permeability of cells increased following 24-h exposure to hypoxia compared to the control group (P<0.01). The knockdown of HIF-2α expression significantly increased occludin and ZO-1 content compared with hypoxia and negative control groups (P<0.01), while permeability was reduced (P<0.01). Hypoxia increased HIF-2α content, inducing permeability of rGENCs through the reduced expression of occludin and ZO-1.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Células Endoteliais/química , Glomérulos Renais/citologia , Ocludina/metabolismo , Proteína da Zônula de Oclusão-1/metabolismo , Animais , Hipóxia Celular/fisiologia , Proliferação de Células , Células Endoteliais/metabolismo , Permeabilidade , Ratos , Fatores de Tempo
11.
Medicine (Baltimore) ; 97(16): e0468, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29668621

RESUMO

RATIONALE: Penetrating neck trauma has a mortality of 3% to 6% and is associated with serious complications, mainly due to the high density of vital structures in this area and the lack of corresponding protection from bone. PATIENT CONCERNS: A 55-year-old man who suffered neck trauma involving the parotid gland, caused by a rebar, after suffering a fall from the second floor of a building. DIAGNOSES: Penetrating neck trauma. INTERVENTIONS: The patient underwent a neck exploration under general anesthesia in the emergency operating room and tracheotomy was performed prophylactically. OUTCOMES: The foreign object was ultimately removed successfully with no complications. LESSONS: We concluded that computed tomography (CT) and three-dimensional reconstruction of the CT images can be a viable method to exclude macrovascular injury for patients who are in an emergency condition and are not eligible for computed tomography angiography (CTA). A chest tube could be used as an option for the removal of foreign bodies in the case of sharp or rough objects (such as spiral foreign bodies), when fully exposing the foreign body and its track.


Assuntos
Esvaziamento Cervical/métodos , Lesões do Pescoço , Glândula Parótida , Ferimentos Penetrantes , Corpos Estranhos/diagnóstico por imagem , Corpos Estranhos/cirurgia , Humanos , Processamento de Imagem Assistida por Computador/métodos , Masculino , Pessoa de Meia-Idade , Lesões do Pescoço/diagnóstico , Lesões do Pescoço/cirurgia , Glândula Parótida/diagnóstico por imagem , Glândula Parótida/lesões , Glândula Parótida/cirurgia , Cuidados Pós-Operatórios , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento , Ferimentos Penetrantes/diagnóstico , Ferimentos Penetrantes/cirurgia
12.
Anal Biochem ; 540-541: 38-44, 2018 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-29128291

RESUMO

OBJECTIVE: To establish a high throughput, low cost, and simple nanotechnology-based method for the detection of single nucleotide polymorphism (SNP) loci in type 2 diabetes mellitus (T2DM). METHODS: Multiplex ligase detection reaction (LDR) amplification was performed using fluorescently labeled magnetic nanosphere-bound upstream LDR probes and downstream probes labeled with a unique fluorescent group for each SNP locus. The amplified LDR products were separated by magnetic nanospheres and then scanned by fluorescence spectroscopy. Four SNP loci associated with T2DM were detected, including the rs13866634 locus in SLC30A8, rs10811661in CDKN2A/2B, rs1111875 in the HHEX gene, and rs7903146 in the TCF7L2 gene. The SNP genotype was also determined by DNA sequencing as a control. RESULTS: The SNP genotypes of the four gene loci determined by the nanosphere-based multiplex LDR method were consistent with the DNA sequencing results. The accuracy rate was 100%. CONCLUSION: A method based on multiplex PCR and LDR was established for simultaneous detection of four SNP loci of T2DM susceptibility genes.


Assuntos
Diabetes Mellitus Tipo 2/diagnóstico , Corantes Fluorescentes/química , Nanosferas/química , Técnicas de Amplificação de Ácido Nucleico , Polimorfismo de Nucleotídeo Único , Adulto , Sequência de Bases , Inibidor de Quinase Dependente de Ciclina p18/química , Inibidor de Quinase Dependente de Ciclina p18/genética , Diabetes Mellitus Tipo 2/genética , Feminino , Genótipo , Proteínas de Homeodomínio/química , Proteínas de Homeodomínio/genética , Humanos , Ligases/metabolismo , Magnetismo , Masculino , Pessoa de Meia-Idade , Análise de Sequência de DNA , Proteína 2 Semelhante ao Fator 7 de Transcrição/química , Proteína 2 Semelhante ao Fator 7 de Transcrição/genética , Fatores de Transcrição/química , Fatores de Transcrição/genética , Transportador 8 de Zinco/química , Transportador 8 de Zinco/genética
13.
Acta Radiol ; 59(6): 672-680, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28870086

RESUMO

Background To clarify the nature of cervical malignant lymphadenopathy is highly important for the diagnosis and differential diagnosis of head and neck tumors. Purpose To investigate the role of first-order apparent diffusion coefficient (ADC) histogram analysis for differentiating lymphoma from metastatic lymph nodes of squamous cell carcinoma (SCC) in the head and neck region. Material and Methods Diffusion-weighted imaging (DWI) data of 67 patients (lymphoma, n = 20; SCC, n = 47) with malignant lymphadenopathy were retrospectively analyzed. The SCC group was divided into nasopharyngeal SCC and non-nasopharyngeal SCC groups. The ADC histogram features (ADC10, ADC25, ADCmean, ADCmedian, ADC75, ADC90, skewness, and kurtosis) were derived and then compared by independent-samples t-test and one-way analysis of variance test, respectively. Receiver operating characteristic curve analyses were employed to investigate diagnostic performance of the significant parameters. Results Lymphoma showed significantly lower ADCmean, ADCmedian, ADC75, and ADC90 than SCC (all P < 0.05). Setting ADC90 = 0.719 × 10-3 mm2/s as the threshold value, optimal diagnostic performance was achieved (area under the curve [AUC] = 0.719, sensitivity = 95.7%, specificity = 50.0%). Subgroup analyses showed no significant difference between lymphoma and NPC (all P > 0.05). Lymphoma showed significantly lower ADC25, ADCmean, ADCmedian, ADC75, and ADC90 than non-nasopharyngeal SCC (all P < 0.05). Optimal diagnostic performance (AUC = 0.847, sensitivity = 86.7%, specificity = 80.0%) could be achieved when setting ADC90 = 0.943 × 10-3 mm2/s as the threshold value. Conclusion Given its limitations, our study has shown that first-order ADC histogram analysis is capable of differentiating lymphoma from metastatic lymph nodes of SCC, especially those of non-nasopharyngeal SCC.


Assuntos
Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/secundário , Neoplasias de Cabeça e Pescoço/patologia , Linfoma/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Diagnóstico Diferencial , Imagem de Difusão por Ressonância Magnética , Feminino , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
14.
Braz. j. med. biol. res ; 51(7): e6201, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889119

RESUMO

This study aimed to investigate the role of hypoxia-inducible factor-2α (HIF-2α) in the expression of tight junction proteins and permeability alterations in rat glomerular endothelial cells (rGENCs) under hypoxia conditions. The expression level of HIF-2α and tight junction proteins (occludin and ZO-1) in rGENCs were examined following 5% oxygen density exposure at different treatment times. HIF-2α lentivirus transfection was used to knockdown HIF-2α expression. Cells were divided into four groups: 1) control group (rGENCs were cultured under normal oxygen conditions), 2) hypoxia group (rGENCs were cultured under hypoxic conditions), 3) negative control group (rGENCs were infected with HIF-2α lentivirus negative control vectors and cultured under hypoxic conditions), and 4) Len group (rGENCs were transfected with HIF-2α lentivirus and cultured under hypoxic conditions). The hypoxia, negative control, and Len groups were kept in a hypoxic chamber (5% O2, 5% CO2, and 90% N2) for 24 h and the total content of occludin and ZO-1, and the permeability of rGENCs were assessed. With increasing hypoxia time, the expression of HIF-2α gradually increased, while the expression of occludin decreased, with a significant difference between groups. ZO-1 expression gradually decreased under hypoxia conditions, but the difference between the 24 and 48 h groups was not significant. The permeability of cells increased following 24-h exposure to hypoxia compared to the control group (P<0.01). The knockdown of HIF-2α expression significantly increased occludin and ZO-1 content compared with hypoxia and negative control groups (P<0.01), while permeability was reduced (P<0.01). Hypoxia increased HIF-2α content, inducing permeability of rGENCs through the reduced expression of occludin and ZO-1.


Assuntos
Animais , Ratos , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Células Endoteliais/química , Glomérulos Renais/citologia , Ocludina/metabolismo , Proteína da Zônula de Oclusão-1/metabolismo , Hipóxia Celular/fisiologia , Proliferação de Células , Células Endoteliais/metabolismo , Permeabilidade , Fatores de Tempo
15.
Exp Ther Med ; 14(1): 753-758, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28672995

RESUMO

Human bocavirus (HBoV) is classified in the Bocavirus genus within the Parvoviridae family, first identified from children with respiratory diseases. Previous studies have investigated the stimulating effect of HBoV on cell apoptosis and autophagy. In the present study, human bronchial epithelial cells (HBECs) were utilized to examine the mechanism of HBoV recombination expressing vector (pWHL-1) on the promotion of cell apoptosis and autophagy. The results from the present study indicated that pWHL-1 inhibited the proliferation of HBECs in a time-dependent manner. Additionally, pWHL-1induced apoptosis, as substantiated by an increased apoptotic rate and presence of autophagosomes. Following pWHL-1 transfection, proliferating cell nuclear antigen, caspase-3 and B cell lymphoma 2 (Bcl-2) protein expression levels were decreased, with the exception of Bcl-2 associated × (Bax) protein, which increased. mRNA and protein expression levels of microtubule-associated protein 1A/1B-light chain 3 (LC3) II and autophagy protein 5 were increased in pWHL-1-transfected HBECs, whereas, the mRNA and protein levels of LC3I and sequestosome 1 were decreased. Notably, pWHL-1 also enhanced the activation of p53 and inhibited AKT activation in HBECs. Results from the present study suggest that pWHL-1 induces apoptosis and autophagy, thus providing a novel insight into the effect of HBoV and its uses in respiratory diseases.

16.
J Huazhong Univ Sci Technolog Med Sci ; 37(3): 412-418, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28585128

RESUMO

Nasal polyp (NP) is a common chronic inflammatory disease of the nasal cavity and sinuses. Although some authors have suggested that NP is related to inflammatory factors such as interleukin (IL)-1ß, IL-5, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor (TNF)-α, and IL-17, the mechanisms underlying the pathogenesis and progression of NP remain obscure. This study investigated the expression and distribution of IL-17 and syndecan-1 in NP, and explored the roles of these two molecules in the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps (Eos CRSwNP) and non-Eos CRSwNP. Real-time PCR and immunohistochemistry were used to detect the expression of IL-17 and syndecan-1 in samples [NP, unciform process (UP) from patients with CRS, and middle turbinate (MT) from healthy controls undergoing pituitary tumor surgery]. The results showed that the expression levels of IL-17 and syndecan-1 were upregulated in both NP and UP tissues, but both factors were higher in NP tissues than in UP tissues. There was no significant difference in IL-17 levels between the Eos CRSwNP and non-Eos CRSwNP samples, and syndecan-1 levels were increased in the non-Eos CRSwNP tissues as compared with those in Eos CRSwNP tissues. In all of the groups, there was a close correlation between the expression of IL-17 and syndecan-1 in nasal mucosa epithelial cells, glandular epithelial cells, and inflammatory cells, suggesting that IL-17 and syndecan-1 may play a role, and interact with each other, in the pathogenesis of non-Eos CRSwNP.


Assuntos
Eosinofilia/genética , Interleucina-17/genética , Pólipos Nasais/genética , Rinite/genética , Sinusite/genética , Sindecana-1/genética , Estudos de Casos e Controles , Doença Crônica , Eosinofilia/imunologia , Eosinofilia/patologia , Células Epiteliais/imunologia , Células Epiteliais/patologia , Regulação da Expressão Gênica , Humanos , Interleucina-17/imunologia , Cavidade Nasal/imunologia , Cavidade Nasal/patologia , Mucosa Nasal/imunologia , Mucosa Nasal/patologia , Pólipos Nasais/complicações , Pólipos Nasais/imunologia , Pólipos Nasais/patologia , Rinite/complicações , Rinite/imunologia , Rinite/patologia , Sinusite/complicações , Sinusite/imunologia , Sinusite/patologia , Sindecana-1/imunologia
17.
Oncotarget ; 8(29): 47230-47238, 2017 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-28525383

RESUMO

PURPOSE: To evaluate the correlation between histogram parameters derived from diffusion-kurtosis (DK) imaging and the clinical stage of nasopharyngeal carcinoma (NPC). RESULTS: High T-stage (T3/4) NPC showed significantly higher Kapp-mean (P = 0.018), Kapp-median (P = 0.029) and Kapp-90th (P = 0.003) than low T-stage (T1/2) NPC. High N-stage NPC (N2/3) showed significantly lower Dapp-mean (P = 0.002), Dapp-median (P = 0.002) and Dapp-10th (P < 0.001) than low N-stage NPC (N0/1). High AJCC-stage NPC (III/IV) showed significantly lower Dapp-10th (P = 0.038) than low AJCC-stage NPC (I/II). ROC analyses indicated that Kapp-90th was optimal for predicting high T-stage (AUC, 0.759; sensitivity, 0.842; specificity, 0.607), while Dapp-10th was best for predicting high N- and AJCC-stage (N-stage, AUC, 0.841; sensitivity, 0.875; specificity, 0.807; AJCC-stage, AUC, 0.671; sensitivity, 0.800; specificity, 0.588). MATERIALS AND METHODS: DK imaging data of forty-seven consecutive NPC patients were retrospectively analyzed. Apparent diffusion for Gaussian distribution (Dapp) and apparent kurtosis coefficient (Kapp) were generated using diffusion-kurtosis model. Histogram parameters, including mean, median, 10th, 90th percentiles, skewness and kurtosis of Dapp and Kapp were calculated. Patients were divided into low and high T, N and clinical stage based on American Joint Committee on Cancer (AJCC) staging system. Differences of histogram parameters between low and high T, N and AJCC stages were compared using t test. Multiple receiver operating characteristic (ROC) curves were used to determine and compare the value of significant parameters in predicting high T, N and AJCC stage, respectively. CONCLUSIONS: DK imaging-derived parameters correlated well with clinical stage of NPC, therefore could serve as an adjunctive imaging technique for evaluating NPC.


Assuntos
Carcinoma/diagnóstico por imagem , Carcinoma/patologia , Imagem por Ressonância Magnética , Neoplasias Nasofaríngeas/diagnóstico por imagem , Neoplasias Nasofaríngeas/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Imagem por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo , Estadiamento de Neoplasias , Variações Dependentes do Observador , Curva ROC , Reprodutibilidade dos Testes , Carga Tumoral , Adulto Jovem
18.
Sci Rep ; 7: 40824, 2017 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-28091620

RESUMO

We report on the optical and structural characterization of rubrene polycrystalline films fabricated from vacuum deposition with various substrate temperatures (Tsub). Depending on Tsub, the role of twisted and planar rubrene conformational isomers on the properties of rubrene films is focused. The temperature (T)-dependent inverse optical transmission (IOT) and photoluminescence (PL) spectra were performed on these rubrene films. The origins of these IOT and PL peaks are explained in terms of the features from twisted and planar rubrene molecules and of the band characteristics from rubrene molecular solid films. Here, two rarely reported weak-peaks at 2.431 and 2.605 eV were observed from IOT spectra, which are associated with planar rubrene. Besides, the T-dependence of optical bandgap deduced from IOT spectra is discussed with respect to Tsub. Together with IOT and PL spectra, for Tsub > 170 °C, the changes in surface morphology and unit cell volume were observed for the first time, and are attributed to the isomeric transformation from twisted to planar rubrenes during the deposition processes. Furthermore, a unified schematic diagram in terms of Frenkel exciton recombination is suggested to explain the origins of the dominant PL peaks performed on these rubrene films at 15 K.

19.
Artigo em Inglês | MEDLINE | ID: mdl-27827944

RESUMO

OBJECTIVE: Asthma is one of the most common chronic diseases and associated with significant morbidity and mortality. However, few data on occupational and environmental risk factors of asthma are available, particularly in Asian adults. Based on a national cross-sectional survey, we assessed the prevalence and risk factors of asthma in Chinese adults. METHODS: A total of 9974 participants aged 15 years and over in seven Chinese cities were selected using a stratified four-stage random sampling. All participants were interviewed face-to-face in their homes using a standardized self-administered questionnaire. Multivariate logistic regression analyses were adopted to determine various risk factors for asthma. RESULTS: The prevalence of self-reported lifetime asthma was 2.46% among the entire adult population, 3.02% among males and 1.93% among females. The prevalence varied by age group, ethnicity, marital status, education, and floor space per person (p < 0.05). After adjusting for socio-demographic variables and smoking, we found independent occupational and environmental determinants of asthma, including a clearance-related job (OR = 2.28, 95%CI: 1.07-4.89), occupational exposure to industrial or occupational poisonous gas (OR = 4.21, 95%CI: 2.43-7.30), having large amounts of carpet in the workplace (OR = 2.61, 95%CI: 1.20-5.69) and using coal for cooking (OR = 2.65, 95%CI: 1.26-5.57). CONCLUSIONS: Asthma is a serious public health problem in China. Our study provides important updated information on the prevalence of asthma and its associated risk factors, which may help us better understand the epidemiology of asthma and prevent this disorder.


Assuntos
Asma/epidemiologia , Exposição Ambiental , Fatores Socioeconômicos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Asma/etiologia , China/epidemiologia , Cidades/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Exposição Ocupacional , Prevalência , Fatores de Risco , Autorrelato , Adulto Jovem
20.
Int Immunopharmacol ; 41: 136-143, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27601384

RESUMO

Myasthenia gravis (MG) is an autoimmune neuromuscular disease characterized by fatigue and muscle weakness. Ginseng is used in the treatment of MG. Ginsenoside Rb1 (G-Rb1), the most abundant ginsenoside in ginseng root, has been proved to be immune regulatory in various diseases. In this study, we investigated the effects and mechanisms of G-Rb1 in treatment for MG in a rat model. Our data showed that G-Rb1 treatment markedly ameliorated the symptoms of experimental autoimmune myasthenia gravis (EAMG) rats, decreased the percentage of Th17 cells in mononuclear cells (MNCs), and increased the number of Treg and Th2 cells in MNCs. We also found that G-Rb1 treatment decreased the serum level of anti-R97-116 peptides IgG1 and IgG2a antibodies. Our findings provide strong evidence that G-Rb1 treatment has immune regulatory effects in EAMG rats, which indicate that G-Rb1 may be employed as a therapeutic medication for MG.


Assuntos
Ginsenosídeos/uso terapêutico , Fatores Imunológicos/uso terapêutico , Miastenia Gravis Autoimune Experimental/tratamento farmacológico , Animais , Citocinas/imunologia , Feminino , Ginsenosídeos/farmacologia , Imunoglobulina G/imunologia , Fatores Imunológicos/farmacologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Linfonodos/citologia , Miastenia Gravis Autoimune Experimental/imunologia , Ratos Endogâmicos Lew , Linfócitos T Reguladores/efeitos dos fármacos , Linfócitos T Reguladores/imunologia , Células Th17/efeitos dos fármacos , Células Th17/imunologia , Células Th2/efeitos dos fármacos , Células Th2/imunologia
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