Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 230
Filtrar
1.
J Ethnopharmacol ; 283: 114635, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34648901

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Huazhuojiedu decoction, a Chinese herbal preparation, has been proven to be clinically effective in treating precancerous lesions in gastric cancer (PLGC). This formula is optimized from a classic formula called "Ganluxiaodu Dan." Although some experiments have shown that Huazhuojiedu decoction is effective against PLGC, the mechanism remains unclear. AIM OF THE STUDY: To investigate the treatment of PLGC with Huazhuojiedu decoction from the perspective of lncRNA in vitro and in vivo. MATERIALS AND METHODS: A PLGC rat model was prepared and randomly divided into a Huazhuojiedu decoction group (HG), a vitacoenzyme group (VG), a model group (MG), and a normal group (CG). Each group was given a corresponding concentration of medicine and distilled water for 10 weeks. The pathological changes in the gastric mucosa were observed by hematoxylin-eosin staining (HE). High-throughput sequencing was performed to detect the differentially expressed lncRNAs in the HG, MG, and CG. Quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) was used to verify differentially expressed lncRNAs, and rat-human homology information was obtained from the University of California, Santa Cruz (UCSC) Genome Database. Human gastric mucosal epithelial cells (GES-1) were used to prepare precancerous lesions of gastric cancer cells (MC). A Huazhuojiedu decoction drug-containing serum was prepared to treat the MC cells. The effects of the Huazhuojiedu decoction and the lncRNA ENST00000517368 (lnc 517368) knockdown or overexpression on PLGC cell proliferation and apoptosis were evaluated in vitro using CCK-8, flow cytometry, and RT-qPCR. RESULTS: The HE results showed that gastric mucosal pathology was significantly improved in the HG. High-throughput sequencing results showed that compared with the CG, 91 lncRNAs upregulated in the MG were restored and downregulated in the HG (P < 0.05), and 115 lncRNAs downregulated in the MG were restored and upregulated in the HG (P < 0.05). The results of RT-qPCR were consistent with the sequencing results. The differentially expressed genomic rat lncRNA ENSRNOT00000079699 is homologous to human lnc 517368. In cell experiments, high expression of lnc 517368 promoted proliferation and reduced apoptosis in PLGC cells, while the Huazhuojiedu decoction reduced the expression of lnc 517368 and improved cell morphology. CONCLUSIONS: Huazhuojiedu decoction inhibited cell proliferation and promoted apoptosis in PLGC cells, and its effect may be partially dependent on the downregulation of lnc 517368.

2.
Talanta ; 237: 122963, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34736688

RESUMO

Endoplasmic reticulum (ER) is an indispensable organelle responsible for protein synthesis, transportation, and maintenance of Ca2+ homeostasis in eukaryotic cells. Recent studies highlighted that ER-targeted photosensitizers with high yield of singlet oxygen (1O2) are effective in selectively disrupting ER function and are promising candidates for anticancer therapy. Unfortunately, no ER targetable fluorescent probes for determining 1O2 photosensitized in this photodynamic therapy process is available. In this work, we synthesized an ER-targetable, two-photon fluorescence probe, ER-1O2, for fluorescence turn-on sensing of 1O2. ER-1O2 demonstrated high sensitivity to 1O2 sensing with a wide detection range (0-2.75 µM) and a low detection limit (0.11 µM). ER-1O2 also displayed excellent selectivity toward 1O2 out of other ROS and metal ions. Notably, ER-1O2 exhibited low cytotoxicity but with specific ER targetable capability. On account of these advantageous features, fluctuations of 1O2 in living cells and brain tissues were effectively visualized by ER-1O2.


Assuntos
Corantes Fluorescentes , Oxigênio Singlete , Encéfalo , Retículo Endoplasmático , Fluorescência
4.
Front Endocrinol (Lausanne) ; 12: 770815, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34867815

RESUMO

The association between hyperuricemia and cardiovascular disease (CVD) has been reported and studied in the past two decades. Xanthine oxidase (XO) induced uric acid (UA) serves as a risk factor and has the independent prognostic and functional impact of heart failure (HF), but whether it plays a positive role in the pathogenesis of HF has remained unclear. Growing evidence suggest the up-regulated XO avtivity and increased production of free oxygen radical (ROS) correspondingly are the core pathogenesis of HF with hyperuricemia, which results in a whole cluster of pathophysiologic cardiovascular effects such as oxidative stress, endothelial dysfunction, vascular inflammation, left ventricular (LV) dysfunction as well as insulin resistance (IR). The use of XO inhibition represents a promising therapeutic choice in patients with HF due to its dual effect of lowering serum UA levels as well as reducing ROS production. This review will discuss the pathophysiologic mechanisms of hyperuricemia with HF, the targeted therapeutic interventions of UA lowering therapies (ULT) with XO inhibition and mechanism underlying beneficial effects of ULT. In addition, the review also summarizes current evidence on the role of ULT in HF and compares CV risk between allopurinol and febuxostat for practical and clinical purposes. Guidelines and implementation of CV risk management in daily practice will be discussed as well.

5.
Chem Asian J ; 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34913596

RESUMO

Amines are an important class of compounds in natural products and medicines. The universal availability of amides provides a potential way for the synthesis of amines. Herein, Ru/Nb2 O5 catalyst is demonstrated to be highly efficient and stable for the selective hydrogenation of propionamide to propylamine (as a model reaction), with up to 91.4% yield of propylamine under relatively mild conditions. Results from XPS analyses, CO chemisorption, TEM images and DRIFTS spectra revealed that the unique properties of Nb2 O5 can effectively activate the C=O group of amides, and the smaller Ru particles on Nb2 O5 could further promote the activation, leading to superior catalytic performance of Ru/Nb2 O5 for amide hydrogenation. Meanwhile, reducing the surface acidity of Nb2 O5 can greatly inhibit the side reactions to by-products, and further enhance the selectivity to amine. Moreover, this catalytic system is also applicable for the hydrogenation of a variety of amides and provides high potential for the industrial production of primary amines from amides.

6.
Artigo em Inglês | MEDLINE | ID: mdl-34839919

RESUMO

The increase in atmospheric carbon dioxide (CO2) concentration has led to numerous problems related to our living environment, seeking an efficient carbon capture and storage (CCS) strategy associated with low energy consumption and expenditures is highly desirable. Here, we demonstrate a facile approach to synthesize a series of highly porous carbon materials derived from porous organic polymers synthesized from three low-cost isomers of triphenyl using chemical activation with KOH at different temperatures. Compared with the precursor porous organic polymers, the porosity of the prepared porous carbon materials is significantly enhanced with surface areas as high as 3367 m2 g-1 and pore volumes up to 1.224 cm3 g-1. Notably, such porous carbon materials deliver an exceptionally high CO2 adsorption capacity of 7.78 mmol g-1 at 273 K and 1 bar, a value that is superior to most of the previously reported adsorbents. In addition, these porous organic polymers and derived porous carbon materials exhibit high CO2/N2 selectivity at ambient conditions. Therefore, the facile construction of highly porous carbon materials from porous organic polymers may offer an efficient strategy for CO2 adsorption and separation and further mitigates greenhouse effect.

7.
Diabetes Metab Res Rev ; : e3514, 2021 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-34841643

RESUMO

1. OBJECTIVE: To explore the relationship between C-peptide and glycemic control rate and diabetic complications(microvascular complication and cerebral infarction), and provide evidence for stratified treatment of type 2 diabetes based C-peptide. 2. METHOD: This is a cross-sectional real-world observational study. According to the inclusion and exclusion criteria, we included 1377 patients with type 2 diabetes mellitus (T2DM), grouped by fasting C-peptide and HOMA-IR. Blood samples were collected after fasting overnight. Logistic regression was used to analyze the relationship among fasting C-peptide, HOMA-IR, C2/C0 ratio, glycemic control rate, and diabetic complications. Restricted cubic spline (RCS) curves based on logistic regression were used to evaluated the relationship between C-peptide, glycemic control rate and DKD. 3. RESULTS: Group Q3 (1.71 ≤C-peptide <2.51ng/mL) has the lowest incidence of DKD, DR and the using rate of insulin, and higher glycemic control rate. Logistic regression shows that, the possibility of not reaching glycemic control in Q3 and Q4 decreased, compared with the group Q1, after adjusting for age, gender, duration of diabetes, BMI, SBP, DBP, Cr, LDL, TG, TC, HDL. Restricted cubic spline (RCS) curve shows that, when C-peptide≤2.68 ng/mL, the incidence of not reaching glycemic control decreases with C-peptide increasing. The possibility of not reaching glycemic control decreased with C2/C0 increasing, when C-peptide≥1.71ng/mL. RCS curve shows that the relationship between C-peptide and DKD present a U-style curve. When C-peptide<2.84 ng/mL, the incidence of DKD decreased with C-peptide increasing. With the C2/C0 ratio increasing, the incidence of DKD, DR, fatty liver didn't decrease. 4. CONCLUSION: When 1.71 ≤C-peptide < 2.51 ng/mL, patients with T2DM had a higher glycemic control rate. Excessive C-peptide plays different roles in DKD and DR, C-peptide may promote the incidence of DKD but protect patients from DR. Higher C2/C0 ratio is important for reaching glycemic control but can't reduce the risk of DKD, DR, and fatty liver. This article is protected by copyright. All rights reserved.

8.
BMC Nephrol ; 22(1): 368, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34742256

RESUMO

BACKGROUND: LncRNA NNT-AS1 (NNT-AS1) has been extensively studied as the causative agent in propagation and progression of lung and bladder cancers, and cholangiocarcinoma. However, its significance in proliferation and inflammation of diabetic nephropathy is enigmatic. This study focuses on the molecular mechanisms followed by NNT-AS1 to establish diabetic nephropathy (DN) and its potential miRNA target. METHODS: Bioinformatics analysis to identify potential miRNA target of NNT-AS1 and smad4 transcription factor was conducted using LncBase and TargetScan, and was subsequently confirmed by luciferase reporter assay. Relative quantitative expression of NNT-AS1 in human glomerular mesangial cells (HGMCs) was detected through quantitative real-time PCR and WB analysis. Cell proliferation was detected through CCK-8 assay, whereas, ELISA was conducted to evaluate the expression of inflammatory cytokines. Following this, relative expression of miR-214-5p and smad4 were confirmed through qRT-PCR and western blot analysis. RESULTS: Results from the experiments manifested up-regulated levels of NNT-AS1 and smad4 in the blood samples of DN patients as well as in HGMCs, whereas, downregulated levels of miR-214-5p were measured in the HGMCs suggesting the negative correlation between NNT-AS1 and miR-214-5p. Potential binding sites of NNT-AS1 showed miR-214-5p as its direct target and NNT-AS1 as potential absorber for this microRNA, in turn increasing the expression of transcription factor smad4. CONCLUSION: The data suggests that NNT-AS1 can be positively used as a potential biomarker and indicator of DN and causes extracellular matrix (ECM) accumulation and inflammation of human mesangial cells.

9.
Medicine (Baltimore) ; 100(46): e27706, 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34797296

RESUMO

BACKGROUND: Hemorrhoids are a common and seriously disruptive condition that seriously affects people's lives in terms of treatment. Injection therapy is an effective minimally invasive scheme for the treatment of grade II-III hemorrhoids, but its clinical application is limited by the adverse reactions caused by injection drugs. Some clinical studies have confirmed the efficacy and safety of Shaobei injection as a traditional Chinese medicine extract. However, there is no standard randomized controlled study to verify its efficacy and explore its potential mechanism. METHODS: This is a prospective, randomized, single blind, parallel controlled trial to study the efficacy of Shaobei injection in the treatment of grade II-III hemorrhoids and its effect on the expression of fibulin-3 and fibulin-5 in fibulin protein family. The patients will be randomly divided into a treatment group and control group. The treatment group will be treated with Shaobei injection, and the control group will be treated with rubber band ligation. The observation indexes include: visual analysis scale, postoperative hospital stay, total use of painkillers, fibulin-3 and fibulin-5, hemorrhoids recurrence, and adverse events. Finally, the data will be statistically analyzed by SPASS 18.0 software. DISCUSSION: This study will compare the efficacy of Shaobei injection with the rubber band ligation method in the treatment of grade II-III haemorrhoids and investigate its effect on the expression of fibulin-3 and fibulin-5 in the fibulin protein family. The results of this study will provide a basis for the clinical use of Paeoniflora injection as an alternative to traditional sclerosing agent in the treatment of grade II-III haemorrhoids.Trial registration: OSF Registration number:DOI 10.17605/OSF.IO/MKVDB.

10.
Bioengineered ; 12(1): 7631-7643, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34605348

RESUMO

CircPRKDC has been disclosed to participate in the tumorigenesis of serval tumors, but the regulatory mechanisms of circPRKDC in GC are still unknown. CircPRKDC, miR-493-5p, and insulin receptor substrate 2 (IRS2) levels were tested by RT-qPCR. The epithelial-mesenchymal transition (EMT)-related protein levels were evaluated via western blot. The cell viability, migration and invasion were evaluated through CCK-8 and Transwell assays. Luciferase reporter and RIP assays were employed to confirm the binding ability between miR-493-5p and circPRKDC or IRS2. CircPRKDC was upregulated in GC samples, and circPRKDC silencing restrained GC cell viability, metastasis, and EMT and suppressed GC tumor growth. Besides, miR-493-5p was a target of circPRKDC, and the repressive impact of circPRKDC knockdown on GC development was neutralized by miR-493-5p inhibition. Moreover, miR-493-5p targeted IRS2 and IRS2 addition rescued the effects of circPRKDC depletion on GC progression. Finally, circPRKDC knockdown could regulate IRS2 expression by targeting miR-493-5p. These results elaborated that circPRKDC accelerated GC development via sponging miR-493-5p and increasing IRS2, which might provide novel potential targets for GC treatment.

11.
Pathol Res Pract ; 227: 153642, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34649054

RESUMO

BACKGROUND: This research focuses on exploring RSK4 protein expression within Clear Cell Renal Cell Carcinoma (ccRCC), based on these investigations on level of expressions coupled with the relevance to clinicopathologic features and clinical outcomes. METHODS: The expression of RSK4 in 48 ccRCC and 20 hydronephrosis samples were under the detection of immunohistochemistry; besides, its relevance to the combination of clinicopathologic features with prognosis was committed in virtue of statistical approaches. RESULTS: The 48 ccRCC samples included 36 (75%, 36/48) positive for RSK4, while the positive rate in hydronephrosis samples were 5 (25%, 5/20). Statistical analysis showed that RSK4 in ccRCC samples express higher expression the hydronephrosis samples (P < 0.05). Furthermore, the expression of RSK4 in ccRCC samples weren't correlated with ages and genders (P > 0.05), while WHO/ISUP nucleolar grade harboured relevance to low survival rate (P = 0.018). Molecular researches demonstrated that over-expression of RSK4 was able to upgrade the proliferation capability of ccRCC cell lines. CONCLUSIONS: According to the expression pattern and molecular systems featured RSK4 in ccRCCs, it performed the function of a latent independent prognostic factor performing the function of a newly built latent therapeutic aim oriented with the patients undergoing RCC. Moreover, the specific mechanism of action is expected to be revealed in the future research.

12.
Front Genet ; 12: 650077, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34497632

RESUMO

We report a single-point variant of low-density lipoprotein receptor (LDLR) in a Chinese proband with a clinical diagnosis of familial hypercholesterolemia (FH) with a comprehensive functional analysis. Target exome capture-based next-generation sequencing was used for sequencing and identification of genomic variants in the LDLR gene. The expression, cellular location, and function of the mutant LDLR were analyzed. Sequencing of LDLR in FH patients indicated a point variant of single-base substitution (G < A) at a position of 2389 in the 16th exon, which led to a loss of the 16th exon in the LDLR messenger RNA. This genomic variant was found to cause exon 16 deletion in the mutant LDLR protein. Subsequent functional analyses showed that the mutant LDLR was retained in the Golgi apparatus and rarely expressed in the cellular membranes of HepG2 cells. Accordingly, the intake ability of HepG2 cells with the mutant LDLR was significantly reduced (P < 0.05). In conclusion, our results suggest that a mutant with a single-base substitution (c. 2389G > A) in the 16th exon of the LDLR gene was associated with miscleavage of messenger RNA and the retention of mutant LDLR in the Golgi apparatus, which revealed a pathogenic variant in LDLR underlying the pathogenesis of FH.

13.
J Clin Endocrinol Metab ; 106(11): 3228-3238, 2021 10 21.
Artigo em Inglês | MEDLINE | ID: mdl-34273152

RESUMO

CONTEXT: How lymph node metastasis (LNM)-associated mortality risk is affected by BRAF V600E in papillary thyroid cancer (PTC) remains undefined. OBJECTIVE: To study whether BRAF V600E affected LNM-associated mortality in PTC. DESIGN, SETTING, AND PARTICIPANTS: We retrospectively analyzed the effect of LNM on PTC-specific mortality with respect to BRAF status in 2638 patients (2015 females and 623 males) from 11 centers in 6 countries, with median age of 46 [interquartile range (IQR) 35-58] years and median follow-up time of 58 (IQR 26-107) months. RESULTS: Overall, LNM showed a modest mortality risk in wild-type BRAF patients but a strong one in BRAF V600E patients. In conventional PTC (CPTC), LNM showed no increased mortality risk in wild-type BRAF patients but a robustly increased one in BRAF V600E patients; mortality rates were 2/659 (0.3%) vs 4/321 (1.2%) in non-LNM vs LNM patients (P = 0.094) with wild-type BRAF, corresponding to a hazard ratio (HR) (95% CI) of 4.37 (0.80-23.89), which remained insignificant at 3.32 (0.52-21.14) after multivariate adjustment. In BRAF V600E CPTC, morality rates were 7/515 (1.4%) vs 28/363 (7.7%) in non-LNM vs LNM patients (P < 0.001), corresponding to an HR of 4.90 (2.12-11.29) or, after multivariate adjustment, 5.76 (2.19-15.11). Adjusted mortality HR of coexisting LNM and BRAF V600E vs absence of both was 27.39 (5.15-145.80), with Kaplan-Meier analyses showing a similar synergism. CONCLUSIONS: LNM-associated mortality risk is sharply differentiated by the BRAF status in PTC; in CPTC, LNM showed no increased mortality risk with wild-type BRAF but a robust one with BRAF mutation. These results have strong clinical relevance.

14.
Materials (Basel) ; 14(13)2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34279268

RESUMO

The construction of a forming limit diagram (FLD) is a conventional approach to obtain limit strains and to evaluate the formability of sheet metal. Appropriate necking criteria should be applied to determine the forming limit curve (FLC) accurately. In recent years, deep research on the determination of the FLC has been carried out; meanwhile, several necking criteria have been proposed. However, the application of inappropriate necking criteria would cause deviations when determining FLCs. In this study, both Marciniak and Nakajima tests were carried out on the AA5086 aluminum sheet to make a comparative investigation of different necking criteria in the determination of FLCs. In the Marciniak test, four existing necking criteria were chosen to construct FLCs, and analyzed in detail. The well-performed time dependent and position dependent methods were selected for the Nakajima test. Meanwhile, the modified Wang method based on the height change of the adjacent points was proposed. The comparative results showed that the time and position dependent methods were relatively conservative in both experiments, while the modified Wang method could identify the onset of localized necking more accurately.

15.
Biol Trace Elem Res ; 2021 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-34275107

RESUMO

The aim of this study is to explore the relationship between serum magnesium (Mg2+) level and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (T2DM). The clinical data of 2222 patients with T2DM, including 713 patients with DR and 1509 patients without DR, between September 2016 and August 2020 in our hospital, were analyzed retrospectively. Further, the role and predictive value of serum Mg2+ on the prevalence of DR were determined through logistic regression and the receiver operating characteristic (ROC) curve respectively. The level of serum Mg2+ was lower in DR group than that in non-DR group (0.92 vs 0.88 mmol/L, P < 0.001). Stratified serum Mg2+ levels into quartiles (Q1-Q4), the first (Q1, Mg2+ ≤ 0.85 mmol/L) and fourth quartile (Q4, ≥ 0.96 mmol/L) represented the lowest and highest quartile, respectively. And the incidence of DR was obviously higher in Q1 and Q2 than that in Q3 and Q4 (50.9% and 30.2% vs 23.5% and 21%, respectively). Logistic regression demonstrated that there remained an independent association between lower serum Mg2+ levels and the occurrence of DR (OR were 3.907 and 1.709 in Q1 and Q2, respectively) no matter whether the interference of confounding variables. ROC curve showed the best cut-off value of serum Mg2+ level in predicting the occurrence of DR was 0.875 mmol/L. Lower Mg2+ levels are related with an increased risk of developing DR. Serum Mg2+ level can be a potential clinical indicator to help identify DR in patients with T2DM.

16.
Chin Med ; 16(1): 37, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33933119

RESUMO

BACKGROUND: Hua-Zhuo-Jie-Du (HZJD), a Chinese herbal prescription consisting of 11 herbs, is commonly used in China to treat chronic atrophic gastritis (CAG). We aimed to determine the effect of HZJD on the microbiome-associated metabolic changes in CAG rats. METHODS: The CAG rat models were induced by 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) combined with irregular fasting and 2% sodium salicylate, which was intragastrically administrated in fasted animals for 24 weeks. The CAG rats in the Chinese medicine (CM) group were administered a daily dose of 14.81 g/kg/day HZJD, and the vitacoenzyme (V) group were administered a daily dose of 0.08 g/kg/day vitacoenzyme. All animals were treated for 10 consecutive weeks, consecutively. Hematoxylin and eosin (H&E) staining was used to assess the histopathological changes in the gastric tissues. An integrated approach based on liquid chromatograph mass spectrometer (LC-MS) metabolic profiling combined with 16S rRNA gene sequencing was carried out to assess the effects of HZJD on CAG rats. Spearman analysis was used to calculate the correlation coefficient between the different intestinal microbiota and the metabolites. RESULTS: The H&E results indicated that HZJD could improve the pathological condition of CAG rats. The LC-MS results indicated that HZJD could significantly improve 21 gastric mucosal tissue perturbed metabolites in CAG rats; the affected metabolites were found to be involved in multiple metabolic pathways, such as the central carbon metabolism in cancer. The results of 16S rRNA gene sequencing indicated that HZJD could regulate the diversity, microbial composition, and abundance of the intestinal microbiota of CAG rats. Following HZJD treatment, the relative abundance of Turicibacter was increased, and the relative abundance of Desulfococcus and Escherichia were decreased in the CM group when compared with the M group. Spearman analysis revealed that perturbed intestinal microbes had a strong correlation with differential metabolites, Escherichia exhibited a negative correlation with l-Leucine, Turicibacter was negatively correlated with urea, and Desulfococcus exhibited a positive correlation with trimethylamine, and a negative correlation with choline. CONCLUSIONS: HZJD could protect CAG by regulating intestinal microbiota and its metabolites.

17.
J Colloid Interface Sci ; 599: 351-359, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33962196

RESUMO

High specific surface area, hierarchical porosity, high conductivity and heteroatoms doping have been considered as the dominating factors of high-performance carbon-based supercapacitors. Inspired by the blue phenomenon of combination of starch and iodine, iodine is employed firstly as pore-making agent to create micropores for the starch-derived carbon in this study. Based on this mechanism, the hierarchically porous carbon is synthesized through simple solvent heating and high-temperature (1000 °C) carbonization, which achieves high specific surface area of 2989 m2 g-1 (an increase of 39.7% compared to that without iodine) and low electrical resistivity of 0.21â€¯Ω·cm. The assembled symmetric supercapacitors, combined with dual redox-active electrolyte (Bi3+ and Br-), deliver the specific capacitance of 1216 F g-1, energy density of 65.4 Wh kg-1, as well as power density of 787.3 W kg-1 at 2 A g-1. In brief, the abundant biomass resource starch is exploited as carbon source, and the iodine sublimation reaction is conducted to provide more micropores to develop high-performance electrodes of supercapacitors.


Assuntos
Carbono , Iodo , Biomassa , Capacitância Elétrica , Porosidade
18.
J Colloid Interface Sci ; 599: 650-660, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33979747

RESUMO

Steam reforming is an effective measure for biomass tar elimination as well as H2-rich syngas (H2 + CO) production. However, the granular or powdery Ni-based catalysts are prone to deactivation, which is caused by inappropriate mass transfer and clogging of catalyst bed. Herein, monolithic wood carbon (WC) with low-tortuosity microchannels is armed with a carbon-coated mesoporous nickel-silica nanocomposite (Ni-SiO2@C) layer via an evaporation-induced self-assembly and calcination procedure for toluene (tar model compound) steam reforming. The quality of the Ni-SiO2@C layer growing on the surface of WC microchannel is affected by the molar ratios of Si/Ni feed. A uniform thin-layer coverage is obtained on the Ni-15SiO2@C/WC (Si/Ni = 15) catalyst, where highly dispersed Ni nanoparticles (average size of 6.6 nm) with appropriate metal-support interaction and remarkable mechanical strength are achieved. The mass transfer, coke resistance, and hydrothermal stability of the Ni-15SiO2@C/WC catalyst were significantly improved by the multilevel structure assembled from the WC microchannels and the secondary ordered SiO2 mesopores. A stable toluene conversion over 97% with an H2 yield of 135 µmol/min was obtained at 600 °C on the Ni-15SiO2@C/WC catalyst. This work opens a new window for facilely constructing high-performance wood carbon-based monolithic tar reforming catalyst.

19.
J Colloid Interface Sci ; 599: 795-804, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33989932

RESUMO

Constructing effective interphase boundary is one of the efficient approaches for improving photocatalytic performances of semiconductor materials. In this work, an anatase/rutile-TiO2 (AR-TiO2) heterophase junction with appropriate carbon content was successfully fabricated via an in-situ phase transformation process. The phase transformation started from the inner core of the nanoparticles and the area of phase interface between anatase and rutile was carefully controlled by regulating the activation temperature. The well-established type-II band alignment between two TiO2 phases with residual carbon as additional charge transfer intermediary which significantly improved the light-harvesting and photoinduced electron-hole pair separation. As a result, the optimal AR-TiO2-550 catalyst (without adding commonly used Pt as co-catalyst) remarkably enhanced photocatalytic H2 generation (201 µmol h-1 g-1), which was about 12-fold to that of P25. The AR-TiO2-550 heterophase junction also showed long-term stability under simulated solar light irradiation. This research provides a new phase engineering route for developing high-efficient photocatalysts.

20.
Int J Rheum Dis ; 24(4): 599-607, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33650318

RESUMO

BACKGROUND: MicroRNAs (miRNAs) have been shown to play a crucial role in inflammation regulation; however, their relationship with inflammation in acute gouty arthritis has not been fully elucidated. Herein, we conducted a study to explore the regulatory roles of miR-223-3p and miR-22-3p in gouty-associated inflammation. METHODS: In vitro and in vivo experiments were conducted to examine the molecular mechanisms of miRNA regulation in gouty inflammation. Dual-luciferase reporter assay was used to verify the direct target of miR-223-3p and miR-22-3p. RESULTS: We found that miR-223-3p and miR-22-3p interacted with the 3' untranslated region segment of NLRP3 (nucleotide-binding domain leucine-rich repeat [NLR] and pyrin domain containing receptor 3) and inhibited its expression. A decreased expression of miR-223-3p and miR-22-3p was observed in both mice air pouch synovium and phorbol myristrate acetate-treated THP-1 cells stimulated with monosodium urate (P < .05). Compared with the negative control group, NLRP3 expression at the transcript and protein level in miR-223-3p and miR-22-3p overexpression group significantly decreased after 6 hours of monosodium urate treatment in vivo and in vitro (P < .05). The results of the dual-luciferase reporter assay demonstrated that miR-223-3p and miR-22-3p directly targeted NLRP3. CONCLUSION: The findings of the present study show that miR-223-3p and miR-22-3p can reduce the inflammatory effects of gout by inhibiting the expression of NLRP3.


Assuntos
Artrite Gotosa/metabolismo , Inflamassomos/metabolismo , Inflamação/metabolismo , MicroRNAs/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Regiões 3' não Traduzidas , Animais , Artrite Gotosa/induzido quimicamente , Artrite Gotosa/imunologia , Artrite Gotosa/prevenção & controle , Sítios de Ligação , Modelos Animais de Doenças , Regulação para Baixo , Humanos , Inflamassomos/genética , Inflamação/induzido quimicamente , Inflamação/imunologia , Inflamação/prevenção & controle , Masculino , Camundongos Endogâmicos BALB C , MicroRNAs/genética , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Transdução de Sinais , Células THP-1 , Ácido Úrico
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...