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1.
Phytomedicine ; 108: 154502, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36274412

RESUMO

BACKGROUND: TYHX-Tongyang Huoxue decoction has been used clinically for nearly 40 years. The ingredients of TYHX are Radix Astragali (Huangqi), Red Ginseng (Hongshen), Rehmannia Glutinosa (Dihuang), Common Yam Rhizome (Shanyao) and Cassia-bark-tree Bark (Rougui). Our previous experiments confirmed that TYHX can protect sinoatrial node cells. However, its mechanism of action is not completely understood yet. PURPOSE: The present study aimed to determine the protective effects of TYHX against Sinus node cell injury under hypoxic stress and elucidate the underlying mechanisms of protection. METHODS: Through RNA sequencing analysis and network pharmacology analysis, we found significant differences in mitochondrial-related genes before and after hypoxia-mimicking SNC, resolved the main regulatory mechanism of TYHX. Through the intervention of TYHX on SNC, a series of detection methods such as laser confocal, fluorescence co-localization, mitochondrial membrane potential and RT-PCR. The regulatory effect of TYHX on ß-tubulin in sinoatrial node cells was verified by in vitro experiments. The mechanism of action of TYHX and its active ingredient quercetin to maintain mitochondrial homeostasis and protect sinoatrial node cells through mitophagy, mitochondrial fusion/fission and mitochondrial biosynthesis was confirmed. RESULTS: Through RNA sequencing analysis, we found that there were significant differences in mitochondrial related genes before and after SNC was modeled by hypoxia. Through pharmacological experiments, we showed that TYHX could inhibit the migration of Drp1 to mitochondria, inhibit excessive mitochondrial fission, activate mitophagy and increase the mitochondrial membrane potential. These protective effects were mainly mediated by ß-tubulin. Furthermore, the active component quercetin in TYHX could inhibit excessive mitochondrial fission through SIRT1, maintain mitochondrial energy metabolism and protect SNCs. Our results showed that protection of mitochondrial function through the maintenance of ß-tubulin and activation of SIRT1 is the main mechanism by which TYHX alleviates hypoxic stress injury in SNCs. The regulatory effects of TYHX and quercetin on mitochondrial quality surveillance are also necessary. Our findings provide empirical evidence supporting the use of TYHX as a targeted treatment for sick sinus syndrome. CONCLUSION: Our data indicate that TYHX exerts protective effects against sinus node cell injury under hypoxic stress, which may be associated with the regulation of mitochondrial quality surveillance (MQS) and inhibition of mitochondrial homeostasis-mediated apoptosis.


Assuntos
Medicamentos de Ervas Chinesas , Sirtuína 1 , Tubulina (Proteína) , Humanos , Hipóxia , Mitocôndrias , Quercetina/farmacologia , Nó Sinoatrial/citologia , Nó Sinoatrial/metabolismo , Sirtuína 1/metabolismo , Tubulina (Proteína)/metabolismo , Medicamentos de Ervas Chinesas/farmacologia
2.
Gen Physiol Biophys ; 41(6): 569-577, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36454117

RESUMO

As COVID-19 spreads over the world, the treatment of acute lung injury (ALI) has attracted much attention. Considering ubiquitin-specific protease (USP) 25 has been relevant to inflammation, this article focused on its role in ALI and its regulatory mechanism. Lipopolysaccharide (LPS) was applied to separately stimulate mice and human lung epithelial cells to establish in vivo and in vitro ALI models. To discover the effects of USP25 overexpression on mouse, lung pathology, inflammatory factor levels, edema, number of inflammatory cells, and downstream protein levels were evaluated. USP25 overexpression in mice could alleviate LPS-induced lung tissue lesions and edema, and reduce inflammatory factors and inflammatory cells. It also inhibited the levels of downstream TRAF6, MAPK pathway-related proteins, and Fos Proto-Oncogene (FOS) in vivo. Furthermore, BEAS-2B cells were transfected with TNF receptor-associated factor 6 (TRAF6) plasmids to study the role of TRAF6 in the regulatory mechanism of USP25. TRAF6 overexpression was found to reverse the functions of USP25 overexpression on cells. In conclusion, USP25 reduced ALI and inhibited inflammation in lung epithelial cells via regulating TRAF6/MAPK/FOS signaling.

3.
Ecotoxicol Environ Saf ; 248: 114278, 2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36379071

RESUMO

Groundwater is a very important natural resource for humanity, however, the degradation of groundwater quality caused by anthropogenic activities may pose a serious hazard to human health and ecosystem. In this study, 143 groundwater samples were analyzed to investigate the spatiotemporal variations of hydrochemistry, groundwater quality and potential human health risk of groundwater in Jinghe River Basin, Chinese Loess Plateau. Based on the mean concentrations value (MCV) and over-standard rate (OSR), the groundwater in Jinghe River Basin were divided into three groups: Group Ⅰ (high MCV: greater than standard values and high OSR: ≥ 10 %) contains TDS (total dissolved solids), TH (total harness), SO42-, F- and Fe; Group Ⅱ (low MCV and high OSR) contains pH, Cl-, NO3-, Cr6+ and As, Group Ⅲ (low MCV and low OSR) contains NO2-, NH4+, Zn, Mn, Pb, I- and Cd. The results of set pair assessment indicated that the unsuitable and doubtful class of comprehensive groundwater quality are mainly distributed in the northern part of study area, accounting for 29 % and 13.6 % of the area in 2004 and 2015, respectively. Human health risk assessment based on the triangular fuzzy number suggested that the variation of risk levels in different age groups is Children > Adult Men > Adult Women. Average carcinogenic risks of As in groundwater for the three groups exceed the acceptable level, and non-carcinogenic risk of NO3- for Children is higher than the acceptable threshold. The findings of this study provide valuable insight into the spatiotemporal characteristics of groundwater quality and potential health risks of polluted groundwater by anthropogenic activities.

4.
BMC Microbiol ; 22(1): 281, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36424543

RESUMO

BACKGROUND: Plants maintain the steady-state balance of the mutually beneficial symbiosis relationship with their endophytic fungi through secondary metabolites. Meanwhile endophytic fungi can serve as biological inducers to promote the biosynthesis and accumulation of valuable secondary metabolites in host plants through a variety of ways. The composition and structure of endophytic fungal community are affected by many factors, including tissues, seasons and so on. In this work, we studied the community diversity, temporal and spatial pattern of endophytic fungi detected from the roots, stems and leaves of Huperzia serrata in different seasons. The correlation between endophytic fungi and huperzine A (HupA) content in plants was analyzed. RESULTS: A total of 7005 operational taxonomic units were detected, and all strains were identified as 14 phyla, 54 classes, 140 orders, 351 families and 742 genera. Alpha diversity analysis showed that the diversity of endophytic fungi in stem and leaf was higher than that in root, and the diversity in summer (August) was lower than that in other months. NMDS analysis showed that the endophytic fungal communities of leaves, stems and roots were significantly different, and the root and leaf communities were also different between four seasons. Through correlation analysis, it was found that 33 genera of the endophytic fungi of H. serrata showed a significant positive correlation with the content of HupA (p < 0.05), of which 13 genera (Strelitziana, Devriesia, Articulospora, Derxomyces, Cyphellophora, Trechispora, Kurtzmanomyces, Capnobotryella, Erythrobasidium, Camptophora, Stagonospora, Lachnum, Golubevia) showed a highly significant positive correlation with the content of HupA (p < 0.01). These endophytic fungi may have the potential to promote the biosynthesis and accumulation of HupA in plant. CONCLUSIONS: This report is the first time to analyze the diversity of endophytic fungi in tissues of H. serrata in different seasons, which proves that there is variability in different tissues and seasonal distribution patterns. These findings provide references to the study of endophytic fungi of H. serrata.


Assuntos
Ascomicetos , Basidiomycota , Huperzia , Micobioma , Humanos , Huperzia/microbiologia , Endófitos
5.
Anal Sci ; 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36427159

RESUMO

We developed a quantitative proton nuclear magnetic resonance (qNMR) with global spectral deconvolution (GSD) method to determine the gamma-aminobutyric acid content in Chinese yam with the proton signal at δH 2.30. Trimethylsilyl-2,2,3,3-tetradeuteropropionic acid sodium salt was set as the internal standard. The method was validated and showed admissible stability, repeatability, and precision. Compared to the traditional high-performance liquid chromatography method, this method did not involve tedious pre-treatment and expensive standard. Compared to ordinary qNMR, GSD algorithm could effectively remove the effect of noise, baseline distortions and signal overlapping. Overall, qNMR with GSD method is a rapid, simple and reliable method to quantitatively determine functional metabolites even overlapped with other compounds in herbs or foods.

6.
Sci Rep ; 12(1): 19634, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36385129

RESUMO

Several single-center studies have evaluated the predictive performance of mechanical power (MP) on weaning outcomes in prolonged invasive mechanical ventilation (IMV) patients. The relationship between MP and weaning outcomes in all IMV patients has rarely been studied. A retrospective study was conducted on MIMIC-IV patients with IMV for more than 24 h to investigate the correlation between MP and weaning outcome using logistic regression model and subgroup analysis. The discriminative ability of MP, MP normalized to dynamic lung compliance (Cdyn-MP) and MP normalized to predicted body weight (PBW-MP) on weaning outcome were evaluated by analyzing the area under the receiver-operating characteristic (AUROC). Following adjustment for confounding factors, compared with the reference group, the Odds Ratio of weaning failure in the maximum MP, Cdyn-MP, and PBW-MP groups increased to 3.33 [95%CI (2.04-4.53), P < 0.001], 3.58 [95%CI (2.27-5.56), P < 0.001] and 5.15 [95%CI (3.58-7.41), P < 0.001], respectively. The discriminative abilities of Cdyn-MP (AUROC 0.760 [95%CI 0.745-0.776]) and PBW-MP (AUROC 0.761 [95%CI 0.744-0.779]) were higher than MP (AUROC 0.745 [95%CI 0.730-0.761]) (P < 0.05). MP is associated with weaning outcomes in IMV patients and is an independent predictor of the risk of weaning failure. Cdyn-MP and PBW-MP showed higher ability in weaning failure prediction than MP.


Assuntos
Estado Terminal , Desmame do Respirador , Humanos , Estado Terminal/terapia , Respiração Artificial , Estudos Retrospectivos , Curva ROC
7.
Breastfeed Med ; 17(11): 940-946, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36378822

RESUMO

Background: The microbial community in human milk is associated with many maternal and neonatal factors. This study aimed to investigate the effect of antibiotic exposure on the microbial community structure of colostrum. Methods: Twenty women with antibiotic treatment immediately after delivery and 10 age-matched control women were enrolled at the Guangdong Women and Children Hospital. Colostrum samples were collected within postpartum 30 hours. The V4 variable region of the bacterial 16S rRNA gene was sequenced to characterize the microbial profile using Illumina MiSeq platform. Results: Phyla Proteobacteria and Firmicutes were the predominant bacteria in colostrum samples. The core and abundant genera in colostrum included Streptococcus, Staphylococcus, and Pseudomonas. Compared with the control group, principal coordinate analysis based on the Bray-Curtis distance showed a significant difference in milk microbial community in women with antibiotic exposure, accompanied by a significantly lower alpha diversity and a different microbial ecological network. Furthermore, the relative abundances of genera Actinomyces, Anaerobacter, and Clostridium_sensu_stricto significantly decreased after antibiotic treatment. Conclusions: This study provided evidence of alterations in the colostrum microbial community with antibiotic exposure, improving our understanding of the effects of antibiotic treatment on the milk microbiome.


Assuntos
Colostro , Microbiota , Gravidez , Recém-Nascido , Criança , Feminino , Humanos , RNA Ribossômico 16S/genética , Antibacterianos/uso terapêutico , Aleitamento Materno , Leite Humano/microbiologia
8.
Theranostics ; 12(17): 7550-7566, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36438474

RESUMO

Rational: Senescence is a major aging process that contributes to the development of cardiovascular diseases, but the underlying molecular mechanisms remain largely unknown. One reason is due to the lack of suitable animal models. We aimed to generate a cardiomyocyte (CM)-specific senescent animal model, uncover the underlying mechanisms, and develop new therapies for aging associated cardiac dysfunction. Methods: The gain/loss of circHIPK3 approach was used to explore the role of circHIPK3 in cardiomyocyte (CM) senescence. To investigate the mechanisms of circHIPK3 function in cardiac senescence, we generated CM-specific tamoxifen-induced circHIPK3 knockout (CKO) mice. We also applied various analyses including PCR, Western blot, nuclear and cytoplasmic protein extraction, immunofluorescence, echocardiography, RNA immunoprecipitation assay, RNA-pulldown assay, and co-immunoprecipitation. Results: Our novel CKO mice exhibited worse cardiac function, decreased circHIPK3 expression and telomere length shortening in the heart. The level of the senescence-inducer p21 in the hearts of CKO mice was significantly increased and survival was poor compared with control mice. In vitro, the level of p21 in CMs was significantly decreased by circHIPK3 overexpression, but increased by circHIPK3 silencing. We showed that circHIPK3 was a scaffold for p21 mRNA-binding protein HuR and E3 ubiquitin ligase ß-TrCP. circHIPK3 silencing weakened the interaction between HuR and ß-TrCP, reduced HuR ubiquitination, and enhanced the interaction between HuR and p21 mRNA. Moreover, we found that mice injected with human umbilical cord mesenchymal stem cell-derived exosomes (UMSC-Exos) showed increased circHIPK3 levels, decreased levels of p21, longer telomere length, and good cardiac function. However, these beneficial effects exerted by UMSC-Exos were inhibited by silencing circHIPK3. Conclusions: We successfully generated CM-specific CKO mice for aging research. Our results showed that deletion of circHIPK3 led to exaggerated CM senescence and decreased cardiac function. As a scaffold, circHIPK3 enhanced the binding of E3 ubiquitin ligase ß-TrCP and HuR in the cytoplasm, leading to the ubiquitination and degradation of HuR and reduced p21 activity. In addition, UMSC-Exos exerted an anti-senescence and cardio-protective effect by delivering circHIPK3. These findings pave the way to the development of new therapies for aging associated cardiac dysfunction.


Assuntos
Cardiopatias , RNA Circular , Humanos , Camundongos , Animais , Ubiquitina/metabolismo , Proteínas Contendo Repetições de beta-Transducina , RNA Mensageiro/metabolismo , RNA , Ubiquitina-Proteína Ligases/genética , Ubiquitina-Proteína Ligases/metabolismo
9.
Front Microbiol ; 13: 1017352, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36386634

RESUMO

Brown leaf spot on tobacco is a serious fungal disease caused by Alternaria alternata. Peroxisomes are organelles playing an important role in the development and infection of plant pathogenic fungi. But, until now, there is no report on the peroxisome dynamics during the conidia germination of A. alternata. To evaluate the roles of peroxisome in the development of the fungus, in the present work, an enhanced green fluorescent protein (eGFP) cassette tagged with peroxisome targeting signal 2 (PTS2) was integrated into A. alternata to label the organelles, and an eGFP cassette carrying a nuclear located signal (NLS) was performed parallelly. The transformants containing the fusions emitted fluorescence in punctate patterns. The fluorescence of eGFP-PTS2 was distributed exactly in the peroxisomes while those of eGFP-NLS were located in the nucleus. Typical AaGB transformants were selected to be investigated for the peroxisome dynamics. The results showed that during spore germination, the number of peroxisomes in the spores decreased gradually, but increased in the germ tubes. In addition, when the transformants were cultured on lipid media, the numbers of peroxisomes increased significantly, and in a larger portion, present in striped shapes. These findings give some clues for understanding the peroxisomal functions in the development of A. alternata.

10.
Materials (Basel) ; 15(21)2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36363289

RESUMO

The advantageous implications of the microstructure and volume fraction of reversed austenite for the tensile properties of super 13Cr martensitic stainless steel (13Cr SMSS) in an experiment with quenching and double-step tempering treatment in the temperature range of 550-750 °C were investigated. The results show that, with increases in one-step tempering temperature, the content of reversed austenite was enhanced considerably from 0.9% to 13.3%. The reversed austenite distributed in the martensitic lath boundary conformed to the (11¯1)γ//(011)α' and [011]γ//[1¯1¯1]α' Kurdjumov-Sachs orientation relationship with the matrix. When tempered at 675 °C for 3 h for the first stage and 600 °C for 2 h for the second stage, the maximum volume fraction of reversed austenite was approximately 13.3%, achieving uniform elongation of 10.4% and total elongation of 27.2%. Moreover, the product of strength and elongation (PSE) was 23.5 GPa·% higher than other samples. The outstanding combination of high strength and commendable plasticity was due to the phase transformation of the reversed austenite into secondary martensite during tensile straining. The reversed austenite consumed the plastic energy at the tip of the microcrack and made the crack tip blunt, which hindered the further propagation of the crack, consequently increasing the total elongation and improving toughness.

11.
Insect Biochem Mol Biol ; 151: 103865, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36336194

RESUMO

Dicers belong to a class of large RNase III multidomain ribonucleases and are central components of the RNA interference (RNAi) pathways. In insects, Dicer-2 has been known to cleave long double-stranded RNA (dsRNA) in small interfering RNA (siRNA)-mediated-RNAi pathway. However, Dicer-1 is responsible for cleaving precursor microRNAs (pre28 miRNAs) in miRNA-mediated RNAi pathway. In this study, we identified one LmDicer-1 and two LmDicer-2 (LmDicer-2a and LmDicer-2b) genes in Locusta migratoria. The RNAi of RNAi assay showed that knockdown of each of the Dicer genes reduced RNAi efficiency against a target gene (Lmß-Tubulin), suggesting that all these genes participated in the siRNA-mediated RNAi pathway. Sequence analyses of the siRNAs generated from dsLmß-Tubulin after silencing each LmDicer gene showed no significant difference in the pattern of siRNAs mapped to dsLmß-Tubulin. This result indicated that all the three LmDicers are capable of generating siRNAs from the dsRNA. We then generated recombinant proteins consisting of different domains using Escherichia coli expression system and incubated each recombinant protein with dsLmß-Tubulin. We found that the recombinant Dicer proteins successfully cleaved dsLmß-Tubulin. However, LmDicer-2a-R lacking dsRBD domain lost activity, suggesting that dsRBD domain is critical for Dicer function. Furthermore, overexpression of these proteins in Drosophila S2 cells improved RNAi efficiency. Our siRNA affinity chromatography and LC-MS/MS analysis identified LmDicer-2a, LmDicer-2b, LmR2D2, LmAgo2a, LmAgo1, LmStaufen and LmTARBP2 as constituents of RNA-induced silencing complex. Taken together, these data show that both LmDicer-1 and two LmDicer-2s all participate in siRNA-mediated RNAi pathway and likely contribute to high RNAi efficiency in L. migratoria.

12.
Dis Markers ; 2022: 1614208, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36246560

RESUMO

Objective: To analyze the differentially expressed genes (DEGs) in rats with endogenous acute respiratory distress syndrome (ARDS) lung injury and explore the pathogenesis and early diagnostic molecular markers using whole transcriptomic data. Methods: Twelve 8-week-old male Sprague Dawley rats were selected and randomly and equally divided into ARDS lung injury group and normal control group. RNA was extracted from the left lung tissues of both the groups and sequenced using the paired-end sequencing mode of the Illumina Hiseq sequencing platform. The DEGs of miRNA, cirRNA, lncRNA, and mRNA were screened using DESeq2 software, and the ceRNA regulatory network was constructed using Cytoscape. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed using the mRNA DEGs. STRING and Cytoscape software were used to construct the protein interaction network and identify the 15 key genes, which were verified using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Based on different screening conditions, and compared with the control group, the ARDS lung injury group showed 836 mRNA DEGs (386 upregulated and 450 downregulated), 110 lncRNA DEGs (53 upregulated and 57 downregulated), 19 circRNA DEGs (3 upregulated and 16 downregulated), and 6 miRNA DEGs (5 upregulated and 1 downregulated gene). GO showed that the DEGs of mRNA were mainly involved in biological processes, such as defense response to lipopolysaccharide and other organisms, leukocyte chemotaxis, neutrophil chemotaxis, and cytokine-mediated signaling. KEGG enrichment analysis showed that the DEGs played their biological roles mainly by participating in IL-17, TNF, and chemokine signaling pathways. The PPI analysis showed a total of 281 node proteins and 634 interaction edges. The top 15 key genes, which were screened, included Cxcl10, Mx1, Irf7, Isg15, Ifit3, Ifit2, Rsad2, Ifi47, Oasl, Dhx58, Usp18, Cmpk2, Herc6, Ifit1, and Gbp4. The ceRNA network analysis showed 69 nodes and 73 correlation pairs, where the key gene nodes were miR-21-3p, Camk2g, and Stx2. Conclusions: The chemotaxis, migration, and degranulation of inflammatory cells, cytokine immune response, autophagy, and apoptosis have significant biological functions in the occurrence and development of endogenous acute lung injury during ARDS. Thus, the camk2g/miR-21-3p/lncRNA/circRNA network, CXCL10/CXCR3, and IL-17 signaling pathways might provide novel insights and targets for further studying the lung injury mechanism and clinical treatment.


Assuntos
Ácidos Nucleicos Livres , Lesão Pulmonar , MicroRNAs , RNA Longo não Codificante , Síndrome do Desconforto Respiratório , Animais , Masculino , Ratos , Quimiocinas/genética , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Interleucina-17/genética , Lipopolissacarídeos , MicroRNAs/genética , Ratos Sprague-Dawley , Síndrome do Desconforto Respiratório/genética , RNA Circular , RNA Longo não Codificante/genética , RNA Mensageiro/genética , Transdução de Sinais , Transcriptoma
13.
Oncogene ; 41(44): 4877-4892, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36192478

RESUMO

PTEN is frequently mutated in human cancers, which leads to the excessive activation of PI3K/AKT signaling and thus promotes tumorigenesis and drug resistance. Met1-linked ubiquitination (M1-Ubi) is also involved in cancer progression, but the mechanism is poorly defined. Here we find that HOIP, one important component of linear ubiquitin chain assembly complex (LUBAC), promotes prostate cancer (PCa) progression by enhancing AKT signaling in a PTEN-dependent manner. Mechanistically, PTEN is modified by M1-Ubi at two sites K144 and K197, which significantly inhibits PTEN phosphatase activity and thus accelerates PCa progression. More importantly, we identify that the high-frequency mutants PTENR173H and PTENR173C in PCa patients showed the enhanced level of M1-Ubi, which impairs PTEN function in inhibition of AKT phosphorylation and cell growth. We also find that HOIP depletion sensitizes PCa cells to therapeutic agents BKM120 and Enzalutamide. Furthermore, the clinical data analyses confirm that HOIP is upregulated and positively correlated with AKT activation in PCa patient specimen, which may promote PCa progression and increase the risk of PCa biochemical relapse. Together, our study reveals a key role of PTEN M1-Ubi in regulation of AKT activation and PCa progression, which may propose a new strategy for PCa therapy.


Assuntos
PTEN Fosfo-Hidrolase , Neoplasias da Próstata , Masculino , Humanos , PTEN Fosfo-Hidrolase/genética , PTEN Fosfo-Hidrolase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Linhagem Celular Tumoral , Neoplasias da Próstata/tratamento farmacológico , Neoplasias da Próstata/genética , Ubiquitinação
14.
Medicine (Baltimore) ; 101(41): e31091, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36254019

RESUMO

Male infertility is a multifactorial condition that is closely associated with chromosomal abnormalities. Reciprocal chromosomal translocation (RCT) is a significant structural genetic abnormality. The specific mechanisms of forms of RCT affecting male infertility include the product of chromosomally unbalanced gametes, thereby disrupting the structure and function of important genes responsible for spermatogenesis. RCT breakpoints have been found to disrupt gene structure and function in many medical fields However, the relationship between RCT breakpoints and male infertility remains to be determined. The purpose of this study is to describe 2 male carriers of RCTs 46,XY,t(8;22)(q13;q13) and 46,XY,t(8;14)(q13;q22). Both patients were collected from the second hospital of Jilin University. Semen parameters were detected using the computer-aided semen analysis system. Cytogenetic analysis was performed using standard operating procedure. Related genes on chromosomal breakpoints were searched using Online Mendelian Inheritance in Man. One man had semen parameters within the normal range, but the couple was infertile after 5 years of marriage. The other man showed normal semen parameters, and his wife had experienced 2 spontaneous miscarriages. Using a literature search, the association between chromosome 22q13 breakpoint and fertility were investigated. The results suggest that physicians should focus on the clinical phenotype of the patients and the breakpoints of RCT in genetic counseling. An important gene related to human male infertility is clearly located in chromosome region 22q13, and its function is worthy of further study.


Assuntos
Infertilidade Masculina , Translocação Genética , Humanos , Masculino , Pontos de Quebra do Cromossomo , Infertilidade Masculina/genética , Cariótipo , Cariotipagem , Translocação Genética/genética , Cromossomos Humanos
15.
Sci Rep ; 12(1): 17215, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36241689

RESUMO

To investigate the safety and effectiveness of trans-oral trans-sheath forceps biopsy (TTFB) for patients with severe esophageal obstruction under fluoroscopy. From November 2016 to November 2019, 35 patients with level III or IV dysphagia and a Karnofsky score of less than 60 were enrolled to undergo TTFB and esophageal nutrition tube insertion or stenting simultaneously. Data on diagnostic performance, early complications, and radiation dose were collected, and Karnofsky scores before and after the procedures were compared. The technical success of TTFB was 100%. The sensitivity, specificity and accuracy were 92.3% (24/26), 77.8% (7/9), and 88.6% (31/35), respectively. Complications occurred in two cases (5.7%). The mean procedure duration and irradiation dose were 23.2 min and 7.2 mSv, respectively. The Karnofsky scores significantly increased after 2-4 weeks (t = 11.22, P < 0.0001). TTFB is a safe and effective method for patients with severe esophageal obstruction under fluoroscopy, especially in those who cannot undergo or refuse endoscopy.


Assuntos
Neoplasias Esofágicas , Estenose Esofágica , Biópsia/efeitos adversos , Neoplasias Esofágicas/complicações , Estenose Esofágica/complicações , Fluoroscopia/métodos , Humanos , Estudos Retrospectivos , Instrumentos Cirúrgicos/efeitos adversos
16.
Vis Comput ; : 1-17, 2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36185464

RESUMO

With the worldwide spread of the COVID-19 pandemic, the demand for medical syringes has increased dramatically. Scale defect, one of the most common defects on syringes, has become a major barrier to boosting syringe production. Existing methods for scale defect detection suffer from large volumes of data requirements and the inability to handle diverse and uncertain defects. In this paper, we propose a robust scale defects detection method with only negative samples and favorable detection performance to solve this problem. Different from conventional methods that work in a batch-mode defects detection manner, we propose to locate the defects on syringes with a two-stage framework, which consists of two components, that is, the scale extraction network and the scale defect discriminator. Concretely, the SeNet is first built to utilize the convolutional neural network to extract the main structure of the scale. After that, the scale defect discriminator is designed to detect and label the scale defects. To evaluate the performance of our method, we conduct experiments on one real-world syringe dataset. The competitive results, that is, 99.7% on F1, prove the effectiveness of our method.

17.
Front Plant Sci ; 13: 994214, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36304403

RESUMO

Pollen morphology, pollen vigor, and long-term pollen storage are critical for plant cross-breeding and genetic improvement of Exochorda racemosa. We developed a protocol for viability determination and storage of E. racemosa pollen for breeding new varieties. The medium components for E. racemosa pollen germination was optimized by using an Orthogonal Assay Test Strategy (OATS). The germination rates of E. racemosa pollen were investigated after storing at different temperatures and different storage periods. The size of E. racemosa pollen was medium with three germination ditches, and the sculptural type of pollen was striate. Red ink and 2,3,5-triphenyl tetrazolium chloride (TTC) can effectively distinguish viable pollen from the unviable pollen of E. racemosa. The most suitable medium (CK2) for E. racemosa was composed of 150 g· L-1 sucrose, 100 mg·L-1 boric acid, 150 mg· L-1 Ca(NO3)2 and 50 mg· L-1 GA3. Low-temperature stress produced the greater inhibition of pollen tube growth compared with high-temperature conditions. The CK2 medium at pH 6.5 resulted in the highest pollen germination rate and most extended pollen tube length. The optimal temperature for storage of dried pollen was -80°C (P < 0.01), and the germination rate was 53.60% after storage for 390 days. Thawing in a 35°C water bath produced the best viability of E. racemosa pollen after storage at -20°C and -80°C. The short-term storage of E. racemosa fresh pollen at 4°C was better than that at -20°C and -80°C (P < 0.01). It is possible to evaluate pollen quality and store pollen grains for E. racemosa by the parameters defined in this study.

18.
Front Pharmacol ; 13: 1012294, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36278160

RESUMO

We conducted a phase I bioequivalence trial in healthy Chinese subjects in the fasting and postprandial states. The goal of this trial was to compare the pharmacokinetics and safety of the test preparation Cefaclor granule (Disha Pharmaceutical Group Co., Ltd.) and the reference preparation Cefaclor suspension (Ceclor®, Eli Lilly and Company). In this trial, 24 subjects were selected in the fasting and postprandial states, respectively. Enrolled subjects randomly accepted a single dose of 0.125 g Cefaclor granule or Cefaclor suspension. The washout period was set as 2 days. Blood samples were collected within 8 h after administration in the fasting state and within 10 h after administration in the postprandial state. Plasma concentrations were determined by Liquid chromatography-tandem mass spectrometry (LC-MS/MS). Pharmacokinetic parameters (AUC, Cmax) were used to evaluate bioequivalence of the two drugs. In the fasting trial, the geometric mean ratios (90% confidence intervals CIs) for Cmax, AUC0-t, and AUC0-∞ were 93.01% (85.96%-100.63%), 97.92% (96.49%-99.38%) and 97.95% (96.52%-99.41%), respectively. The GMR (90% CIs) for Cmax, AUC0-t, and AUC0-∞ in postprandial state were 89.27% (81.97%-97.22%), 97.31% (95.98%-98.65%) and 97.31% (95.93%-98.71%), respectively. The 90% CIs of AUC and Cmax in the fasting and postprandial states were within the 80-125% bioequivalence range. Therefore, Cefaclor granule and Cefaclor suspension were bioequivalent and displayed similar safety profiles. Furthermore, food intake affected the pharmacokinetic parameters of both drugs.

19.
Front Plant Sci ; 13: 956083, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36299788

RESUMO

Physalins, active ingredients from the Physalis alkekengi L. var. franchetii (P. alkekengi) plant, have shown anti-inflammatory, antioxidant and anticancer activities. Whereas the bioactivity of physalins have been confirmed, their biosynthetic pathways, and those of quite a few derivatives, remain unknown. In this paper, biosynthesis and structure modification-related genes of physalins were mined through transcriptomic and metabolomic profiling. Firstly, we rapidly and conveniently analyzed physalins by UPLC-Q-TOF-MS/MS utilizing mass accuracy, diagnostic fragment ions, and common neutral losses. In all, 58 different physalin metabolites were isolated from P. alkekengi calyxes and berries. In an analysis of the physalin biosynthesis pathway, we determined that withanolides and withaphysalins may represent a crucial intermediate between lanosterol and physalins. and those steps were decanted according to previous reports. Our results provide valuable information on the physalin metabolites and the candidate enzymes involved in the physalins biosynthesis pathways of P. alkekengi. In addition, we further analyzed differential metabolites collected from calyxes in the Jilin (Daodi of P. alkekengi) and others. Among them, 20 physalin metabolites may represent herb quality biomarkers for Daodi P. alkekengi, providing an essential role in directing the quality control index of P. alkekengi.

20.
J Exp Bot ; 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36306203

RESUMO

Locoweeds are leguminous forbs known for their toxicity to livestock caused by the endophytic fungi Alternaria sect. Undifilum. Unlike the defensive mutualism reported in many toxin producing endophytes and their plant hosts, the benefits that A. sect. Undifilum can confer to it host plants has been unclear. Here we conducted physiological and genetic analyses to show that A. (sect. Undifilum) oxytropis influences the growth, especially root development in its locoweed host and Arabidopsis. A. oxytropis significantly decreased primary root length while increasing the number of lateral roots and root hairs, plant leaf area and fresh weight. It enhanced the levels of plant endogenous auxin, and the expression of key genes in auxin biosynthesis, signaling and transport. Such influence on root development was abolished in auxin signaling and polar transport mutants. A. oxytropis down-regulated PIN1 but increased PIN2, PIN7, and AUX1, which may reflect altered spatial auxin accumulation responsible for the change in root architecture. Plant growth was also insensitive to A. oxytropis when NPA (naphthylphthalamic acid) was applied. Our findings indicate a function of A. oxytropis in promoting the growth and development of Arabidopsis via the regulation of auxin. This suggests the possible role of A. oxytropis to benefit its locoweed hosts, via a process irrelevant to its toxin production.

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