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1.
Front Public Health ; 10: 933851, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35910905

RESUMO

Nocardia endophthalmitis is a relatively uncommon form of endophthalmitis seen in clinical patients. In general, Nocardia endophthalmitis tends to carry a poor prognosis. Here, we report a 3-year-old child who was admitted to the hospital due to a rupture of the left eye. The suturing and anterior chamber formation were performed immediately. Approximately, 16 days after the operation, massive whitish plump and tufted exudates gathered in the pupil area and at the bottom of the anterior chamber, and the child was diagnosed with endophthalmitis. The infection was initially considered to be caused by fungal pathogens for that the hyphae and spores were observed in the smear. However, the isolate obtained after 4 days of culturation was identified as actinomycetes using MALDI-TOF. We further classified it as Nocardia huaxiensis by next-generation sequencing (NGS) based on the MinION platform. Amikacin and sulfamethoxazole tablets were used to control the infection and the ocular inflammation subsided gradually. Intraocular lens (IOL) implantation is planned to be performed at an appropriate future time to improve his vision. Nocardia endophthalmitis is rare and usually caused by ocular trauma or surgery. In conclusion, Nocardia huaxiensis should be considered as an emerging pathogen and deserves more attention.


Assuntos
Endoftalmite , Nocardiose , Nocardia , Antibacterianos/uso terapêutico , Pré-Escolar , Endoftalmite/diagnóstico , Endoftalmite/tratamento farmacológico , Endoftalmite/microbiologia , Olho , Humanos , Nocardiose/diagnóstico , Nocardiose/tratamento farmacológico , Nocardiose/microbiologia
2.
Front Aging Neurosci ; 14: 943380, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35923549

RESUMO

Background: Cognitive impairment (CI) has become a worldwide health problem. The relationship between CI and uric acid (UA) is contradictory. Objective: We included participants with a full spectrum of CI, from cognitively unimpaired (CU) to dementia, from the Chongqing Ageing & Dementia Study (CADS). Methods: First, we identified the relationships between serum UA (sUA) and cognitive function in different stages of CI. Second, we analyzed these relationships among different stages and types of CI. Finally, we explored the association between sUA and amyloid/tangle/neurodegeneration (ATN) biomarkers. Results: We recruited 427 participants from the CADS, including 382 participants with mini-mental state examination (MMSE) evaluation. The levels of sUA were positively correlated with MMSE scores (p < 0.001), and the correlation was prominent in the course of dementia and in the type of Alzheimer's disease (AD). The levels of UA had a positive correlation with plasma amyloid-ß 42 (Aß42) (p = 0.004). Higher levels of sUA weakened the correlation of MMSE scores with CSF ATN biomarkers and the correlation of CSF Aß42 with tau. Conclusion: UA is positively correlated with cognitive function, especially in the advanced stage of AD. The probable neuroprotective effects of sUA mainly act on Aß42 and the downstream pathological cascade.

3.
Org Lett ; 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35930708

RESUMO

A novel and efficient copper-catalyzed transannular ring-closing reaction of eight-membered rings has been developed that provides a straightforward way to synthesize bicyclo[3.3.0]octane derivatives in good yields. Mechanistic studies revealed that the reaction pathway might involve chlorination followed by the Kornblum reaction. Readily accessible starting materials and good functional group tolerance make this procedure attractive.

4.
Int J Mol Sci ; 23(15)2022 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-35955571

RESUMO

Melatonin (MT) can effectively reduce oxidative damage induced by abiotic stresses such as salt in plants. However, the effects of MT on physiological responses and molecular regulation during wheat germination remains largely elusive. In this study, the response of wheat seeds to MT under salt stress during germination was investigated at physiological and transcriptome levels. Our results revealed that application of MT significantly reduced the negative influence of salt stress on wheat seed germination. The oxidative load was reduced by inducing high activities of antioxidant enzymes. In parallel, the content of gibberellin A3 (GA3) and jasmonic acid (JA) increased in MT-treated seedling. RNA-seq analysis demonstrated that MT alters oxidoreductase activity and phytohormone-dependent signal transduction pathways under salt stress. Weighted correlation network analysis (WGCNA) revealed that MT participates in enhanced energy metabolism and protected seeds via maintained cell morphology under salt stress during wheat seed germination. Our findings provide a conceptual basis of the MT-mediated regulatory mechanism in plant adaptation to salt stress, and identify the potential candidate genes for salt-tolerant wheat molecular breeding.

5.
Expert Rev Anti Infect Ther ; : 1-15, 2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35790112

RESUMO

OBJECTIVES: Vibrio cholerae O1/O139 is responsible for cholera epidemics that remains a huge public health menace across the globe. Furthermore, an increasing resistance rate among V. cholerae strains has been reported around the world. Therefore, the objective of this meta-analysis was to evaluate the weighted pooled resistance (WPR) rates in clinical V. cholerae O1/O139 isolates based on different years, areas, antimicrobial susceptibility testing, and resistance rates. RESEARCH DESIGN AND METHODS: We searched the studies in PubMed, Scopus, Embase, and Web of Science (until January 2020). Statistical analyses were conducted using STATA software (ver. 14.0). RESULTS: A total of 139 studies investigating 24,062 V. cholerae O1/O139 isolates were analyzed. The majority of the studies originated in Asia (n = 102). The WPR rates were as follows: azithromycin 1%, erythromycin 36%, ciprofloxacin 3%, cotrimoxazole 79%, doxycycline 7%, and tetracycline 20%. There was increased resistance to cotrimoxazole, ciprofloxacin, and tetracycline during the 1980-2020 years. CONCLUSIONS: Temporal changes in antibiotic resistance rate found in this study demonstrated the critical continuous surveillance of antibiotic resistance. Also, ciprofloxacin, azithromycin, gentamicin, cephalexin, imipenem, ofloxacin, and norfloxacin were found to be the best antibiotics against V. cholera, with the highest and the lowest effectiveness resistance rate.

6.
Cancer Res ; 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35895805

RESUMO

Hepatocellular carcinoma (HCC) is one of the primary liver malignancies with a poor prognosis. Glutamic-oxaloacetic transaminase 2 (GOT2) is a highly tissue-specific gene in the liver, but the roles GOT2 plays in the progression of HCC remain unclear. Here, we report that GOT2 is downregulated in HCC tumor tissues and that low expression of GOT2 is associated with advanced progression and poor prognosis. In HCC cells, knockdown of GOT2 promoted proliferation, migration, and invasion. In mouse models of HCC, loss of GOT2 promoted tumor growth as well as hematogenous and intrahepatic metastasis. Mechanistically, silencing of GOT2 enhanced glutaminolysis, nucleotide synthesis, and GSH synthesis by reprogramming glutamine metabolism to support the cellular antioxidant system, which activated the PI3K/AKT/mTOR pathway to contribute to HCC progression. Furthermore, HCC with low expression of GOT2 was highly dependent on glutamine metabolism and sensitive to the glutaminase inhibitor CB-839 in vitro and in vivo. Overall, GOT2 is involved in glutamine metabolic reprogramming to promote HCC progression and may serve as a therapeutic and diagnostic target for HCC.

7.
Clin Ther ; 2022 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-35872028

RESUMO

PURPOSE: This study evaluates the tolerability and efficacy of cyclophosphamide (CYC) for the treatment of refractory chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). METHODS: We searched PubMed, Embase, Cochrane Library, and 2 Chinese databases (Chinese National Knowledge Infrastructure and SinoMed) for studies published between database inception and September 30, 2021. Articles obtained using the appropriate keywords were independently selected by 2 reviewers on the basis of the established inclusion and exclusion criteria. FINDINGS: In total, 240 records that were eventually curtailed to 13 studies with 83 patients were retrieved and subsequently included in this evaluation. All 13 studies were included in the systematic review, 7 of which were included in the meta-analysis. The pooled estimate of the response rate was 68% (95% CI, 45%-90%). The pooled estimate of the adverse reaction rate was 8% (95% CI, 0%-15%). The disease duration before the first CYC treatment was negatively correlated with the reduction in the modified Rankin Scale score (r = -0.76, P < 0.001). However, the response rates did not differ significantly between patients of different sexes (P = 0.716) or between patients who received and those who did not receive concurrent glucocorticoids (P = 0.617). IMPLICATIONS: CYC might be a recommended therapeutic option for patients with refractory CIDP, especially those who are unable to accept rituximab treatment. Earlier CYC treatment yields better therapeutic outcomes in patients with refractory CIDP without CYC-related contraindications.

8.
J AOAC Int ; 2022 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-35876857

RESUMO

BACKGROUND: Rumex japonicus Houtt (R. japonicus) is mainly used to treat various skin diseases in Southeast Asia. However, there are few researches on its quality evaluation methods and anti-fungal activity. OBJECTIVE: To establish the quality control criteria for the effective parts from R. japonicus against psoriasis. METHOD: High-performance liquid chromatography (HPLC) was established for its fingerprint, the similarity evaluation, cluster (CA) and principal component analysis (PCA) were used to reveal the differences of those fingerprints among the tested R. japonicus. Network pharmacology analyzed the relationship between the components and psoriasis, revealing the potential targets of R. japonicus. Oxford cup anti-candida albicans experiment was used to verify the antifungal activity of R. japonicus. RESULTS: HPLC was developed for R. japonicus fingerprint by optimizing for 10 batches of quinquennial R. japonicus from different habitats, the 18 common peaks were identified with 10 characteristic peaks such as rutin, quercetin, aloe-emodin, nepodin, emodin, musizin-8-O-ß-D-glucoside, chrysophanol, emodin-8-O-ß-D-glucopyranoside, chrysophanol-8-O-ß-D-glucopyranoside, and aloin, respectively; The network pharmacology-based analysis showed a high correlation between R. japonicus and psoriasis, revealing the potential targets of R. japonicus. The oxford cup anti-candida albicans experiment displayed a significant activity response to emodin-8-O-ß-D-glucopyranoside and the ethyl acetate fraction of R. japonicus acidic aqueous extract. CONCLUSIONS: A new and optimized HPLC method was created, the research provides an experimental basis for the development of effective drugs related to candida albicans. HIGHLIGHTS: The fingerprint of R. japonicus was organically combined with network pharmacology to further clarify its criteria for quality control.

9.
Inorg Chem ; 61(29): 11284-11292, 2022 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-35834349

RESUMO

Broadband near-infrared (NIR) phosphors are necessary materials for developing portable NIR light sources. Moreover, exploiting an NIR phosphor with a main peak located beyond a wavelength of 900 nm remains a challenge because this spectral range has great potential in biological nondestructive testing and solution testing. In this study, a range of Cr3+-doped ZnTa2O6 (ZTO) phosphors were completely synthesized by a solid-state method, which show broadband Cr3+ emission centered at 935 nm with a large full width at half maximum (FWHM) of 185 nm due to two distorted octahedral sites. A packaged phosphor-converted light-emitting diode (pc-LED) device is used to penetrate a 5-cm-thick chicken breast and identify diverse solutions based on differences in the measured transmission spectra. The results indicate broad application prospects in the field of biological tissue penetration and solution analysis.

10.
NPJ Microgravity ; 8(1): 28, 2022 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-35902585

RESUMO

Screen channel liquid acquisition devices (LADs) are among the most promising technologies for separating liquid and vapor phases in propellant storage tanks under microgravity conditions and thus ensuring vapor-free propellant supply to spacecraft engines. However, the prediction of the critical flow rate of a screen channel LAD relies on the full understanding of the three dimensional distribution of injection velocity. In this study, the flow characteristics at the entrance region of the LAD were investigated via particle image velocimetry (PIV) technique and numerical simulations under various working conditions. The experimental results illustrated that the velocity component normal to the porous woven mesh is non-uniform in both streamwise and spanwise directions of channel flow and that this phenomenon has a significant influence on the critical flow rate. Hence, a model that accounts for the three-dimensional flow field was proposed to predict the critical flow rate. The average error in the critical flow rate, which was determined by comparing the proposed model's predictions and the experimental results, was less than 8.4%.

11.
J Chromatogr A ; 1677: 463304, 2022 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-35809524

RESUMO

A method was developed for the determination of 33 antibiotics belonging to 4 different antibiotic groups, including sulfonamides (16), fluoroquinolones (12), macrolides (1), and tetracyclines (4) in water samples using on-line solid-phase extraction-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (on-line SPE-UPLC-MS/MS). The enrichment and analysis conditions were optimized for the determination of trace concentrations (nanogram per liter). Aliquots of the water samples (5 mL) were filtered through a membrane and enriched on an on-line polymeric column with hydrophilic-lipophilic balance (HLB). The analyte was eluted by the mobile phase during on-line SPE and separated on an Acquity BEH130 column, detected by tandem mass spectrometry, and quantified using an external standard method. The optimization of the analytical methods was discussed, which included optimization of pH of the sample, filtration membrane, Na2EDTA, chromatographic column, formic acid and aqueous ammonia in mobile phase. The detection limit for all test compounds by this method was in the range of 0.2-1.5 ng/L, with recoveries of 76.6-118%. The precision of the method, as indicated by the relative standard deviation, was 2.4-7.9%. Results of analysis of surface water samples demonstrated the ability of the proposed method to analyze ultra-trace levels of antibiotics, without the need for complex manual pretreatment.


Assuntos
Antibacterianos , Espectrometria de Massas em Tandem , Antibacterianos/análise , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida/métodos , Extração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos , Água
12.
Sci Rep ; 12(1): 13054, 2022 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-35906290

RESUMO

The shift quality of an automatic transmission directly affects the human-perceived comfort and the durability of the automatic transmission. In general, the inconsistency caused by manufacturing errors, life-cycle changes, or other changes in hydraulic characteristics are the main reason affecting the shift quality, which should be compensated by adaptive control in the shifting process. In this paper, we first provide an in-depth analysis of the relationship between proportional solenoid current, clutch pressure, speed and torque in the shifting process control. Then we propose two efficient adaptive control strategies for the torque phase and inertia phase, respectively. Both algorithms are tested and verified on a riot utility vehicle. The experimental results indicate that the adaptive control strategies proposed in this paper can effectively compensate the engine flare and the clutch tie-up of the torque phase, and keep the inertia phase within a proper time range.


Assuntos
Algoritmos , Humanos , Torque
13.
Angew Chem Int Ed Engl ; : e202209849, 2022 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-35876073

RESUMO

Electrochemical reduction of biomass-derived 5-hydroxymethylfurfural (HMF) represents an elegant route toward sustainable value-added chemicals production that circumvents the use of fossil fuel and hydrogen. However, the reaction efficiency is hampered by the high voltage and low activity of electrodes (Cu, Bi, Pb). Herein, we report a Ru1 Cu single-atom alloy (SAA) catalyst with isolated Ru atoms on Cu nanowires that exhibits an electrochemical reduction of HMF to 2,5-dihydroxymethylfuran (DHMF) with promoted productivity (0.47 vs. 0.08 mmol cm-2 h-1 ) and faradic efficiency (FE) (85.6 vs. 71.3 %) at -0.3 V (vs. RHE) compared with Cu counterpart. More importantly, the FE (87.5 %) is largely retained at high HMF concentration (100 mM). Kinetic studies by using combined electrochemical techniques suggest disparate mechanisms over Ru1 Cu and Cu, revealing that single-atom Ru promotes the dissociation of water to produce H* species that effectively react with HMF via an electrocatalytic hydrogenation (ECH) mechanism.

14.
J Clin Neurosci ; 103: 14-19, 2022 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-35797769

RESUMO

It is well-established that complete expansion and proper apposition to the vessel wall of flow-diverter stents are critical for optimizing endovascular aneurysm outcomes by using flow diversion techniques. We aimed to evaluate the clinical value of high-resolution cone-beam CT (HR-CBCT) upon flow-diverter stent implantation in intracranial aneurysms. In this study, we retrospectively analyzed the clinical data of eighty-one patients (101 intracranial aneurysms) who underwent flow-diverter stent implantation (Pipeline™ or Tubridge™). Images were reconstructed using conventional cone-beam CT (CBCT)(voxel size 0.43 mm isotropic) and HR-CBCT(voxel size 0.15 mm isotropic). Immediately after stent deployment, dual volume 3D fusion images were obtained from 3D-digital subtraction angiography (DSA) and HR-CBCT. The image quality for stent visualization was graded from 0 to 2 (0:not able to assess, 1:limited, but able to assess; 2:clear visualization), and the stent expansion status (full, under-expanded or poor apposition) was also recorded. Finally, patients were treated using flow-diverter stents (n = 92: 17 Pipeline and 75 Tubridge). Compared to CBCT, HR-CBCT led to improved visualization of the structures of the stents and significantly improved the image quality (mean score: 0.59 ± 0.67 vs. 1.6 ± 0.63, P < 0.001). For 28 stents (seven Pipeline and 21 Tubridge), partially incomplete apposition was observed by HR-CBCT but not by conventional CBCT and resolved by microguidewire looping dilation or balloon dilation. High-resolution cone-beam CT could better display flow-diverter stent details and yielded an improved image quality, which facilitated the assessment of stent deployment, potentially reducing the incidence of complications.

15.
Biomaterials ; 287: 121645, 2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35779480

RESUMO

Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a high mortality rate. Immunotherapy has achieved promising clinical results in multiple cancers, but shows unsatisfactory outcome in GBM patients, and poor drug delivery across the blood-brain barrier (BBB) is believed to be one of the main limitations that hinder the therapeutic efficacy of drugs. Herein, a new cationic lipid nanoparticle (LNP) that can efficiently deliver siRNA across BBB and target mouse brain is prepared for modulating the tumor microenvironment for GBM immunotherapy. By designing and screening cationic LNPs with different ionizable amine headgroups, a lipid (named as BAMPA-O16B) is identified with an optimal acid dissociation constant (pKa) that significantly enhances the cellular uptake and endosomal escape of siRNA lipoplex in mouse GBM cells. Importantly, BAMPA-O16B/siRNA lipoplex is highly effective to deliver siRNA against CD47 and PD-L1 across the BBB into cranial GBM in mice, and downregulate target gene expression in the tumor, resulting in synergistically activating a T cell-dependent antitumor immunity in orthotopic GBM. Collectively, this study offers an effective strategy for brain targeted siRNA delivery and gene silencing by optimizing the physicochemical property of LNPs. The effectiveness of modulating immune environment of GBM could further be expanded for potential treatment of other brain tumors.

16.
Biomaterials ; 287: 121664, 2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35810540

RESUMO

The influenza epidemics pose a significant threat to public health. Of them, type B influenza coincided with several severe flu outbreaks. The efficacy of the current seasonal flu vaccine is limited due to the antigenicity changes of circulating strains. In this study, we generated structure-stabilized HA stalk antigens from influenza B and fabricated double-layered protein nanoparticles as universal influenza B vaccine candidates. In vitro studies found that the resulting protein nanoparticles were effectively taken up to activate dendritic cells. Nanoparticle immunization induced broadly reactive immune responses conferring robust and sustained cross-immune protection against influenza B virus strains of both lineages. The results reveal the potential of layered protein nanoparticles incorporated with structure-stabilized constant antigens as a universal influenza vaccine with improved immune protective potency and breadth.

17.
J Colloid Interface Sci ; 627: 969-977, 2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35905583

RESUMO

As an emerging post-graphene two-dimensional material, black phosphorus (BP) has attracted enormous interest as a promising cocatalyst for photocatalytic hydrogen (H2) evolution, however, the activity of either pristine bulk or BP nanosheets is far from satisfactory. Herein, we present an effective strategy to greatly boost the H2 evolution performance of BP via applying the synergistic effect of heterojunction and interfacial lattice strain. A multilayered heterostructure coupling BP nanosheets and nickel oxide (NiO) nanosheets with abundant interface P-Ni and PO bonds is synthesized and utilized as a proof-of-concept material for our design. Both the experimental and theoretical results have revealed that the strain is formed in BP-NiO multilayered heterostructure. The generated lattice strain induces the charge redistribution at the interface between BP and NiO, which leads to the improved electron transfer efficiency and favorable H* adsorption kinetics for photocatalytic H2 evolution reaction. As a result, the BP-NiO heterostructure with strain effect exhibits much enhanced photocatalytic H2 evolution activity in the presence of Eosin Y (EY) as photosensitizer, exceeding that of zero-strained BP/NiO heterostructure and many other reported noble-metal-free cocatalyst.

18.
ACS Appl Mater Interfaces ; 14(30): 35105-35113, 2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35867633

RESUMO

Insect growth regulators (IGRs) guide animal development through injection, oral feeding, or topical application. Among them, lufenuron is a widely used insect cuticle inhibitor but only shows a gastric toxic effect. Lacking contact toxicity limits the effective utilization when spraying the lufenuron pesticide. To overcome this shortcoming, a nanocarrier (star polycation, SPc)-based transdermal delivery system was applied to improve the penetrability and contact toxicity of lufenuron. The fluoride groups in lufenuron could interact with the tertiary amines in the branch-chain of the SPc through electrostatic interaction to form a lufenuron/SPc complex. The above interaction reduced the particle size of lufenuron from 933 to 70 nm. Interestingly, the contact toxicity of SPc-loaded lufenuron was remarkably improved with effects of higher larval mortality and lower egg hatching rate of the devastating pest fall armyworm. The physiological and molecular toxic mechanism was revealed by RNA-Seq analysis. The SPc-loaded lufenuron apparently down-regulated cuticle-related genes and thus inhibited insect cuticle formation. Such contact toxicity was achieved by the transdermal nanodelivery of lufenuron, which up-regulated endocytosis-related genes for drug uptake. This study is the first successful application of a nanoparticle-mediated transdermal delivery system to explore the contact toxicity of an IGR, which alters the IRG's action mode from oral feeding to topical application.


Assuntos
Inseticidas , Praguicidas , Animais , Insetos , Hormônios Juvenis/farmacologia , Larva , Sistemas de Liberação de Fármacos por Nanopartículas , Praguicidas/farmacologia
19.
Front Cell Infect Microbiol ; 12: 919903, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35837476

RESUMO

Metagenomic next-generation sequencing (mNGS) is a novel useful strategy that is increasingly used for pathogens detection in clinic. Some emerging mNGS technologies with long-read ability are useful to decrease sequencing time and increase diagnosed accuracy, which is of great significance in rapid pathogen diagnosis. Reliable DNA extraction is considered critical for the success of sequencing; hence, there is thus an urgent need of gentle DNA extraction method to get unbiased and more integrate DNA from all kinds of pathogens. In this study, we systematically compared three DNA extraction methods (enzymatic cell lysis based on MetaPolyzyme, mechanical cell lysis based on bead beating, and the control method without pre-cell lysis, respectively) by assessing DNA yield, integrity, and the microbial diversity based on long-read nanopore sequencing of urine samples with microbial infections. Compared with the control method, the enzymatic-based method increased the average length of microbial reads by a median of 2.1-fold [Inter Quartile Range (IQR), 1.7-2.5; maximum, 4.8) in 18 of the 20 samples and the mapped reads proportion of specific species by a median of 11.8-fold (Inter Quartile Range (IQR), 6.9-32.2; maximum, 79.27]. Moreover, it provided fully (20 of 20) consistent diagnosed results to the clinical culture and more representative microbial profiles (P < 0.05), which all strongly proves the excellent performance of enzymatic-based method in long-read mNGS-based pathogen identification and potential diseases diagnosis of microbiome related.


Assuntos
Sequenciamento por Nanoporos , DNA , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Metagenoma , Metagenômica/métodos
20.
Opt Lett ; 47(14): 3471-3474, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35838706

RESUMO

We improve an inverse regular perturbation (RP) model using a machine learning (ML) technique. The proposed learned RP (LRP) model jointly optimizes step-size, gain and phase rotation for individual RP branches. We demonstrate that the proposed LRP can outperform the corresponding learned digital back-propagation (DBP) method based on a split-step Fourier method (SSFM), with up to 0.75 dB gain in a 800 km standard single mode fiber link. Our LRP also allows a fractional step-per-span (SPS) modeling to reduce complexity while maintaining superior performance over a 1-SPS SSFM-DBP.

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