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1.
Ying Yong Sheng Tai Xue Bao ; 30(3): 1015-1024, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30912395

RESUMO

As the key and hot topic in landscape ecology, ecological security pattern plays an important role in maintaining regional ecological security and achieving regional sustainable development. Based on land use data of 2016, we used the InVEST model to evaluate the habitat quality of Tang County to determine the ecological source. The resistance surface was constructed by selecting the resistance factors such as land use type, habitat quality index, vegetation coverage, distance to water, distance to settlements, distance to roads, with the resistance threshold being used to partition the ecological security pattern. Finally, the ecological security pattern of Tang County was constructed by determining the ecological corridor with the MCR model. The results showed that the ecological source of Tang County accounted for 3.3% of the total area, mainly distributed in forest land and waters with large plaque area, and the Western Ocean Reservoir (one of the four major reservoirs in Hebei Province). According to the cost resistance mutation point, the research region could be divided into prohibited development zone, restricted development zone, optimized development zone, and key development zone. The percentage of each zone was 18.9%, 43.6%, 27.6% and 9.9%, respectively. The total length of the potential ecological corridors in Tang County was 333.52 km, and was 263.91 km after optimization, which was helpful for various ecological exchanges. Our results had important guiding significance for the rational and sustainable use of land resources in Tang County, and could provide theoretical and technical supports for the decision-making of land planning and layout in Tang County.


Assuntos
Ecossistema , China , Florestas
2.
Microb Pathog ; 117: 310-314, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29481973

RESUMO

Florfenicol is an antibiotic, a fluorinated structural analogue of thiamphenicol and chloramphenicol, approved exclusively for use in Asia for aquaculture since the 1980's. Our study examined the prevalence of florfenicol resistance in bovine mastitis Escherichia coli isolates. A total of 245 E. coli isolates were collected from bovine mastitis in Ningxia Province, China between May 2016 to July 2017 and screened for florfenicol resistance gene, floR gene by PCR analysis. About 7.35% (15/245) of the isolates were positive for floR gene. Minimal inhibitory concentration (MIC) results showed that 9 isolates were susceptible strains and 6 isolates were highly resistant to florfenicol. HPLC (high performance liquid chromatography) results showed that the amounts of florfenicol was significantly improved in the presence of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) as an efflux pump inhibitor. This, therefore, indicates that the employment of florfenicol in conjunction with CCCP in future drug formulations should be considered.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Proteínas de Escherichia coli/genética , Escherichia coli/genética , Genes Bacterianos/genética , Tianfenicol/análogos & derivados , Animais , Carbonil Cianeto m-Clorofenil Hidrazona , Bovinos , China , Combinação de Medicamentos , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Feminino , Mastite Bovina/microbiologia , Testes de Sensibilidade Microbiana , Tianfenicol/farmacologia
3.
Sci Rep ; 7(1): 9701, 2017 08 29.
Artigo em Inglês | MEDLINE | ID: mdl-28851901

RESUMO

Although alcohol is an established risk factor of head and neck cancer (HNC), insufficiencies exist in the literature in several aspects. We analyzed detailed alcohol consumption data (amount and type of alcoholic beverage) of 811 HNC patients and 940 controls to evaluate the association between alcohol and HNC by HNC sites and by genotypes of ADH1B and ALDH2. Alcohol was associated with an increased HNC risk in a dose-response relationship, with the highest risk observed for hypopharyngeal cancer, followed by oropharyngeal and laryngeal cancers. Liquor showed a stronger positive association with HNC than beer and wine. The highest HNC risk occurred in individuals with the slow ADH1B and slow/non-functional ALDH2 genotype combination. In our study population, 21.8% of HNCs, 55.7% of oropharyngeal cancers, and 89.1% of hypopharyngeal cancers could be attributed to alcohol. Alcohol accounted for 47.3% of HNCs among individuals with the slow ADH1B and slow/non-functional ALDH2 genotype combination. The HNC risk associated with alcohol became comparable to that of never/occasional drinkers after ten or more years of cessation from regular alcohol drinking. In conclusion, alcohol use is associated with an increased HNC risk, particularly for individuals with slow ethanol metabolism. HNC incidence may be reduced by alcohol cessation.


Assuntos
Consumo de Bebidas Alcoólicas , Neoplasias de Cabeça e Pescoço/epidemiologia , Neoplasias de Cabeça e Pescoço/etiologia , Consumo de Bebidas Alcoólicas/efeitos adversos , Alelos , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Polimorfismo de Nucleotídeo Único , Vigilância da População , Medição de Risco , Fatores de Risco , Taiwan/epidemiologia
4.
BMC Cancer ; 17(1): 286, 2017 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-28431509

RESUMO

BACKGROUND: Although substantial evidence supports a 20-30% risk reduction of colon cancer, breast cancer, and endometrial cancer by physical activity (PA), the evidence for head and neck cancer (HNC) is limited. Three published studies on the association between PA and HNC have generated inconsistent results. The current study examined the association between recreational PA (RPA) and HNC risk with a more detailed assessment on the intensity, frequency, duration, and total years of RPA. METHODS: Data on RPA were collected from 623 HNC cases and 731 controls by in-person interview using a standardized questionnaire. The association between RPA and HNC risk was assessed using unconditional logistic regression, adjusted for sex, age, educational level, use of alcohol, betel quid, and cigarette, and consumption of vegetables and fruits. RESULTS: A significant inverse association between RPA and HNC risk was observed in a logistic regression model that adjusted for sex, age, and education (odds ratio (OR) = 0.65, 95% confidence interval (CI): 0.51-0.82). However, after further adjustment for the use of alcohol, betel quid, and cigarette, and consumption of vegetables and fruits, RPA was no longer associated with HNC risk (OR =0.97, 95% CI: 0.73-1.28). No significant inverse association between RPA and HNC risk was observed in the analysis stratified by HNC sites or by the use of alcohol, betel quid, or cigarette. CONCLUSION: Results from our study did not support an inverse association between RPA and HNC risk. The major focus of HNC prevention should be on cessation of cigarette smoking and betel chewing, reduction of alcohol drinking, and promotion of healthy diet that contains plenty of fruits and vegetables.


Assuntos
Exercício Físico/fisiologia , Neoplasias de Cabeça e Pescoço/epidemiologia , Consumo de Bebidas Alcoólicas/epidemiologia , Estudos de Casos e Controles , Fumar Cigarros/epidemiologia , Feminino , Humanos , Modelos Logísticos , Masculino
5.
PLoS One ; 11(10): e0164937, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27780233

RESUMO

OBJECTIVES: Although betel quid (BQ) is an established risk factor of head and neck cancer (HNC), insufficiencies exist in the literature regarding the dose-response, BQ types, HNC sites, and BQ cessation. The current study was conducted to fill these insufficiencies. MATERIALS AND METHODS: A hospital-based case-control study was conducted to evaluate the association between BQ and HNC. In-person interview was conducted to collect data on BQ chewing. The current analysis included 487 men newly diagnosed with HNC and 617 male controls who were frequency-matched to the cases by age. The association between BQ and HNC was assessed using multivariable unconditional logistic regression. RESULTS: Ever BQ chewing was associated with an increased HNC risk regardless of the BQ types. A non-linear positive association between BQ and HNC was observed, with a steep rise in HNC risk for the first 5 pack-years or 200,000 minutes of BQ consumption. Every year of BQ cessation was associated with a 2.9% reduction in HNC risk; however, the risk did not reduce to the level of non-BQ chewers even after 20 years of BQ cessation. Eliminating BQ chewing may prevent 51.6% of HNCs, 62.6% of oral cancers, and 41.3% of pharyngeal cancers in Taiwan. CONCLUSION: Our results supported the positive association between BQ and HNC. BQ cessation is effective in reducing HNC risk and should be encouraged. Because BQ cessation may not reduce the HNC risk to the level of non-BQ chewers, it is important to prevent the initiation of BQ chewing.


Assuntos
Carcinoma de Células Escamosas/epidemiologia , Neoplasias de Cabeça e Pescoço/epidemiologia , Piper betle/efeitos adversos , Fumar/epidemiologia , Estudos de Casos e Controles , Humanos , Modelos Logísticos , Masculino , Mastigação , Pessoa de Meia-Idade , Fatores de Risco , Fumar/efeitos adversos , Carcinoma de Células Escamosas de Cabeça e Pescoço , Taiwan/epidemiologia
6.
Cancer Causes Control ; 27(9): 1105-15, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27443169

RESUMO

PURPOSE: Allergy symptoms have been associated with a reduced head and neck cancer (HNC) risk, while elevated blood immunoglobulin E (IgE) levels have been associated with an increased HNC risk. According to the "prophylaxis hypothesis," allergic reaction is the body's way of expelling carcinogens. IgE level may be increased by exposure to environmental carcinogens, including alcohol and cigarette smoke. We hypothesized that individuals with elevated serum IgE without allergy symptoms (i.e., asymptomatic atopic) would have the highest HNC risk. METHODS: A case-control study of HNC (576 cases and 740 controls) was conducted to evaluate the association between allergy symptoms or serum total IgE and HNC risk and the effect modification of allergy symptoms on the association between serum total IgE and HNC risk. RESULTS: Elevated serum total IgE was associated with a significantly increased HNC risk [odds ratio (OR) 1.71, 95 % confidence interval (CI) 1.21-2.42]. Having allergy symptoms was associated with a significantly reduced HNC risk (OR 0.56, 95 % CI 0.43-0.73). Compared to subjects with normal serum total IgE and no allergy symptoms, asymptomatic atopic individuals had a significantly increased HNC risk (OR 2.12, 95 % CI 1.33-3.35). CONCLUSIONS: Our results provided further evidence to support the "prophylaxis hypothesis." Further investigations regarding the immune profiles of asymptomatic atopic individuals may provide additional clues for the biological mechanisms underlying the association between allergy symptoms, IgE, and HNC risk.


Assuntos
Neoplasias de Cabeça e Pescoço/epidemiologia , Hipersensibilidade/epidemiologia , Imunoglobulina E/sangue , Estudos de Casos e Controles , Feminino , Neoplasias de Cabeça e Pescoço/sangue , Neoplasias de Cabeça e Pescoço/imunologia , Humanos , Hipersensibilidade/sangue , Hipersensibilidade/imunologia , Incidência , Masculino , Pessoa de Meia-Idade , Risco
7.
Mol Med Rep ; 14(3): 2038-44, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27431484

RESUMO

ADAM10 (a disintegrin and metalloprotease 10) is a member of the ADAMs family, which is key in the development of the nervous system, by regulating proliferation, migration, differentiation and survival of various cells, including axonal growth and myelination. Previous studies have investigated the embryonic or postnatal expression of ADAM10, however, detailed information regarding its cellular distribution in the adult stage, to the best of our knowledge, is not available. The present study investigated the expression pattern of the ADAM10 gene in the adult mouse central nervous system (CNS) using an ADAM10 complementary RNA probe for in situ hybridization (ISH). Immunohistochemical staining was used to identify the type of the ISH staining­positive cells with neuron­ or astrocyte­specific antibodies. The results of the current study demonstrated that the ADAM10 gene was predominantly expressed in the neurons of the cerebral cortex, hippocampus, thalamus and cerebellar granular cells in adult mouse CNS.


Assuntos
Proteína ADAM10/metabolismo , Sistema Nervoso Central/metabolismo , Proteína ADAM10/genética , Animais , Cerebelo/metabolismo , Cérebro/metabolismo , Feminino , Expressão Gênica , Imuno-Histoquímica/métodos , Hibridização In Situ/métodos , Masculino , Camundongos , Neurônios/metabolismo , Especificidade de Órgãos/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
8.
Medicine (Baltimore) ; 94(26): e1064, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26131827

RESUMO

This analysis evaluated the association between serum retinol levels and risk of head and neck cancer (HNC) and whether the association is modulated by the use of alcohol, betel quid, or cigarette. In addition, we also examined the association between HNC risk and 2 single nucleotide polymorphisms, TTR rs1667255 and RBP4 rs10882272, that have been associated with serum retinol levels. Unconditional logistic regression was performed to evaluate the association between serum retinol levels and HNC risk among 160 HNC cases and 198 controls. The associations between TTR rs1667255 and RBP4 rs10882272 and serum retinol levels or HNC risk were evaluated by linear regression and unconditional logistic regression, respectively, for 418 HNC cases and 497 controls. The results showed that HNC cases had a lower mean serum retinol level compared with controls (845.3 µg/L vs 914.8 µg/L, P = 0.03). An inverse association between serum retinol levels and HNC risk occurred among never/occasional alcohol drinkers but not among regular drinkers. TTR rs1667255 was associated with serum retinol levels; however, neither TTR rs1667255 nor RBP4 rs10882272 was associated with HNC risk. In summary, this study showed an inverse association between serum retinol levels and HNC risk, specifically among never/occasional alcohol drinkers. More studies are needed to establish the underlying biologic mechanisms for the inverse association between serum retinol levels and HNC risk and the modulation of this relationship by alcohol drinking.


Assuntos
Consumo de Bebidas Alcoólicas/efeitos adversos , Carcinoma de Células Escamosas/sangue , Neoplasias de Cabeça e Pescoço/sangue , Fumar/efeitos adversos , Vitamina A/sangue , Areca , Carcinoma de Células Escamosas/etiologia , Estudos de Casos e Controles , Neoplasias de Cabeça e Pescoço/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Piper betle , Polimorfismo de Nucleotídeo Único , Proteínas Plasmáticas de Ligação ao Retinol/genética
9.
BMC Genomics ; 16: 22, 2015 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-25612663

RESUMO

BACKGROUND: Regional specificity allows different skin regions to exhibit different characteristics, enabling complementary functions to make effective use of the integumentary surface. Chickens exhibit a high degree of regional specificity in the skin and can serve as a good model for when and how these regional differences begin to emerge. RESULTS: We used developing feather and scale regions in embryonic chickens as a model to gauge the differences in their molecular pathways. We employed cosine similarity analysis to identify the differentially regulated and co-regulated genes. We applied low cell techniques for expression validation and chromatin immunoprecipitation (ChIP)-based enhancer identification to overcome limited cell availabilities from embryonic chicken skin. We identified a specific set of genes demonstrating a high correlation as being differentially expressed during feather and scale development and maturation. Some members of the WNT, TGF-beta/BMP, and Notch family known to be involved in feathering skin differentiation were found to be differentially regulated. Interestingly, we also found genes along calcium channel pathways that are differentially regulated. From the analysis of differentially regulated pathways, we used calcium signaling pathways as an example for further verification. Some voltage-gated calcium channel subunits, particularly CACNA1D, are expressed spatio-temporally in the skin epithelium. These calcium signaling pathway members may be involved in developmental decisions, morphogenesis, or epithelial maturation. We further characterized enhancers associated with histone modifications, including H3K4me1, H3K27ac, and H3K27me3, near calcium channel-related genes and identified signature intensive hotspots that may be correlated with certain voltage-gated calcium channel genes. CONCLUSION: We demonstrated the applicability of cosine similarity analysis for identifying novel regulatory pathways that are differentially regulated during development. Our study concerning the effects of signaling pathways and histone signatures on enhancers suggests that voltage-gated calcium signaling may be involved in early skin development. This work lays the foundation for studying the roles of these gene pathways and their genomic regulation during the establishment of skin regional specificity.


Assuntos
Galinhas/genética , Pele/metabolismo , Animais , Canais de Cálcio Tipo L/genética , Canais de Cálcio Tipo L/metabolismo , Diferenciação Celular/genética , Embrião de Galinha , Galinhas/metabolismo , Cromatina/metabolismo , Imunoprecipitação da Cromatina , Plumas/metabolismo , Genoma , Histonas/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Transdução de Sinais , Fator de Crescimento Transformador beta/metabolismo
10.
PLoS One ; 9(5): e96507, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24796481

RESUMO

BACKGROUND: The current study evaluated the association between tea consumption and head and neck cancer (HNC) in Taiwan, where tea is a major agricultural product and a popular beverage. METHODS: Interviews regarding tea consumption (frequency, duration, and types) were conducted with 396 HNC cases and 413 controls. Unconditional logistic regression was performed to estimate the odds ratio (OR) and 95% confidence interval (CI) of HNC risk associated with tea drinking, adjusted for sex, age, education, cigarette smoking, betel quid chewing, and alcohol drinking. RESULTS: A reduced HNC risk associated with tea drinking (OR for every cup per day = 0.96, 95% CI: 0.93-0.99; OR for ≧5 cups per day = 0.60, 95% CI: 0.39-0.94) was observed. The association was especially significant for pharyngeal cancer (OR for every cup per day = 0.93, 95% CI: 0.88-0.98; OR for ≧5 cups per day = 0.32, 95% CI: 0.16-0.66). A significant inverse association between HNC and tea consumption was observed particularly for green tea. CONCLUSIONS: This study suggests that tea drinking may reduce the risk of HNC. The anticancer property of tea, if proven, may offer a natural chemopreventive measure to reduce the occurrence of HNC.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Neoplasias de Cabeça e Pescoço/prevenção & controle , Chá , Quimioprevenção , Feminino , Neoplasias de Cabeça e Pescoço/epidemiologia , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Medição de Risco , Taiwan/epidemiologia
11.
Int J Cancer ; 135(10): 2424-36, 2014 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-24719202

RESUMO

Alcohol consumption is an established risk factor for head and neck cancer (HNC). The major carcinogen from alcohol is acetaldehyde, which may be produced by humans or by oral microorganisms through the metabolism of ethanol. To account for the different sources of acetaldehyde production, the current study examined the interplay between alcohol consumption, oral hygiene (as a proxy measure for the growth of oral microorganisms), and alcohol-metabolizing genes (ADH1B and ALDH2) in the risk of HNC. We found that both the fast (*2/*2) and the slow (*1/*1+ *1/*2) ADH1B genotypes increased the risk of HNC due to alcohol consumption, and this association differed according to the slow/non-functional ALDH2 genotypes (*1/*2+ *2/*2) or poor oral hygiene. In persons with the fast ADH1B genotype, the HNC risk associated with alcohol drinking was increased for those with the slow/non-functional ALDH2 genotypes. For those with the slow ADH1B genotypes, oral hygiene appeared to play an important role; the highest magnitude of an increased HNC risk in alcohol drinkers occurred among those with the worst oral hygiene. This is the first study to show that the association between alcohol drinking and HNC risk may be modified by the interplay between genetic polymorphisms of ADH1B and ALDH2 and oral hygiene. Although it is important to promote abstinence from or reduction of alcohol drinking to decrease the occurrence of HNC, improving oral hygiene practices may provide additional benefit.


Assuntos
Álcool Desidrogenase/genética , Consumo de Bebidas Alcoólicas/efeitos adversos , Aldeído Desidrogenase/genética , Carcinoma de Células Escamosas/etiologia , Neoplasias de Cabeça e Pescoço/etiologia , Higiene Bucal/efeitos adversos , Polimorfismo Genético/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Aldeído-Desidrogenase Mitocondrial , Carcinoma de Células Escamosas/epidemiologia , Estudos de Casos e Controles , Feminino , Seguimentos , Genótipo , Neoplasias de Cabeça e Pescoço/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Prognóstico , Fatores de Risco , Fumar/efeitos adversos , Adulto Jovem
12.
Oral Oncol ; 49(10): 1010-7, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23948049

RESUMO

OBJECTIVES: This analysis examined the association between oral hygiene and head and neck cancer (HNC) and whether this association differed by the consumption of alcohol, betel quid, or cigarette and by the genetic polymorphisms of inflammation-related genes. MATERIALS AND METHODS: Interviews regarding dental care and oral health were conducted with 317 HNC cases and 296 controls. Genotyping was performed for 6 single nucleotide polymorphisms in IL6, IL10 and PTGS2. RESULTS: A positive association was observed between HNC and no regular dental visits (odds ratio (OR)=2.86, 95% confidence interval (CI): 1.47-5.57), brushing teeth <2times/day (OR=1.51, 95% CI: 1.02-2.23), frequent gum bleeding (OR=3.15, 95% CI: 1.36-7.28), and loss of >20 teeth (OR=2.31, 95% CI: 1.05-5.07). Analysis with dental care score (range: 0-4, 4=worst dental care), which combined regular dental visits, toothbrushing, and use of dental floss and mouthwash, showed a positive trend with HNC risk, particularly among alcohol drinkers and cigarette smokers. Multifactor dimensionality reduction analysis divided the study subjects into high- and low-risk group based on combinations of dental care score and IL6 rs1800796 genotypes. Compared to the low-risk group, the high-risk group had an OR of HNC=2.16 (95% CI: 1.44-3.25). CONCLUSIONS: This study observed a positive association between poor oral hygiene and HNC, which appeared to differ by alcohol or cigarette consumption and the genotypes of IL6 rs1800796. Further investigations are needed to determine whether poor oral hygiene is a cause for HNC or a surrogatemarker of an unhealthy lifestyle that increases the risk of HNC.


Assuntos
Carcinoma de Células Escamosas/epidemiologia , Neoplasias Laríngeas/epidemiologia , Neoplasias Bucais/epidemiologia , Higiene Bucal/estatística & dados numéricos , Neoplasias Faríngeas/epidemiologia , Consumo de Bebidas Alcoólicas/epidemiologia , Estudos de Casos e Controles , Feminino , Humanos , Interleucina-6/genética , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Fatores de Risco , Fumar/epidemiologia , Taiwan/epidemiologia , Uso de Tabaco/epidemiologia
13.
PLoS One ; 8(2): e55138, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23383309

RESUMO

BACKGROUND: Although the relationship between allergy and cancer has been investigated extensively, the role of allergy in head and neck cancer (HNC) appears less consistent. It is not clear whether allergies can independently influence the risk of HNC in the presence of known strong environmental risk factors, including consumption of alcohol, betel quid, and cigarette. METHODS: THE CURRENT PAPER REPORTS RESULTS FROM: 1) an original hospital-based case-control study, which included 252 incident cases of HNC and 236 controls frequency-matched to cases on sex and age; and 2) a meta-analysis combining the results of the current case-control study and 13 previously published studies (9 cohort studies with 727,569 subjects and 550 HNC outcomes and 5 case-control studies with 4,017 HNC cases and 10,928 controls). RESULTS: In the original case-control study, we observed a strong inverse association between allergies and HNC [odds ratio = 0.41, 95% confidence interval (CI): 0.27-0.62]. The meta-analysis also indicated a statistically significant inverse association between HNC and allergies [meta-relative risk (RR) = 0.76, 95% CI: 0.63-0.91], particularly strong for allergic rhinitis (meta-RR = 0.55, 95% CI: 0.40-0.76). In addition, the inverse association between allergies and HNC was observed only among men (meta-RR = 0.67, 95% CI: 0.54-0.84) but not among women (meta-RR = 0.98, 95% CI: 0.81-1.18). CONCLUSIONS: These findings suggest that immunity plays an influential role in the risk of HNC. Future studies investigating immune biomarkers, including cytokine profiles and genetic polymorphisms, are warranted to further delineate the relationship between allergies and HNC. Understanding the relationship between allergies and HNC may help devise effective strategies to reduce and treat HNC.


Assuntos
Neoplasias de Cabeça e Pescoço/epidemiologia , Hipersensibilidade/epidemiologia , Consumo de Bebidas Alcoólicas/efeitos adversos , Areca/efeitos adversos , Estudos de Casos e Controles , Feminino , Neoplasias de Cabeça e Pescoço/etiologia , Neoplasias de Cabeça e Pescoço/imunologia , Humanos , Hipersensibilidade/imunologia , Modelos Logísticos , Masculino , Razão de Chances , Fatores Sexuais , Fumar/efeitos adversos , Inquéritos e Questionários , Taiwan/epidemiologia
14.
Artigo em Chinês | MEDLINE | ID: mdl-23072129

RESUMO

OBJECTIVE: To construct a recombinant vector for rapid gene tagging in Giardia lamblia. METHODS: To obtain the recombinant vector pGL gdh-Neo with the Neo selection marker, the Neo gene was put under the control of gdh promoter by overlap PCR and inserted into pGEM-5zf. A DNA fragment containing multiple cloning sites (MCS) followed by triple hemagglutinin(3HA) coding sequences was synthesized and cloned into the pGL gdh-Neo to construct a recombinant vector pGL MCS-3HA-gdh-Neo. Giardia H2A gene was selected as a tagging gene to validate the effectivity of the recombinant vector pGL MCS-3HA-gdh-Neo. The histone H2A coding sequence was amplified by PCR, digested with EcoR I and Spe I, and inserted into MCS of pGL MCS-3HA-gdh-Neo. The resulting plasmid was then linearized and transfected into Giardia trophozoites. The H2A recombinant strain selected by G418 was analyzed by PCR,Western blotting and immunofluorescence. RESULTS: A rapid tagging recombinant vector with multiple cloning sites and triple hemagglutinin (3HA) was constructed with a length of 4 260 bp. The H2A recombinant vector was transfected into Giardia trophozoites and integrate into the Giardia genome at the correct locus. The HA-tagged H2A protein was expressed with a molecular weight (Mr) of 16 900. CONCLUSION: A rapid tagging recombinant vector of genes in Giardia lamblia, pGL MCS-3HA-gdh-Neo, has been constructed.


Assuntos
Vetores Genéticos , Giardia lamblia/genética , Clonagem Molecular , Expressão Gênica , Plasmídeos , Transfecção
15.
Cell Biochem Biophys ; 62(1): 41-6, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21833674

RESUMO

The goal of this retrospective study was to determine the effect of para-aortic lymphadenectomy on clinical outcome in patients with stage N+ rectal adenocarcinoma below the peritoneal reflection. A retrospective analysis was performed on the clinical outcome of 181 patients with stage N+ rectal adenocarcinoma below the peritoneal reflection who underwent total mesorectal excision (TME) with total pelvic lymph node (PLN) adenectomy, with or without para-aortic lymph node (PAN) adenectomy. Independent prognostic factors were determined by multivariate Cox regression analysis. Disease-free survival (DFS) was analyzed using Kaplan-Meier curves and the log-rank test. The incidence of PLN metastases was 39.2% (71/181) in all the patients, and the incidence of PAN metastases was 12% (12/100) in patients who received PLN + PAN adenectomies. The patients were divided into two groups: PLN adenectomy (n = 81) and PLN + PAN adenectomy (n = 100). There were no statistically significant differences in clinicopathological factors between the PLN adenectomy and PLN + PAN adenectomy groups. On univariate analysis, the gross tumor type (P = 0.012), histological differentiation (P = 0.013), CEA level (P = 0.019), T stage (P = 0.019), N stage (P < 0.0001), and the number of positive PLN sites (P < 0.0001) were associated with poor DFS. Gross tumor type (P = 0.031), N stage (P = 0.001), and the number of positive PLN sites (P < 0.0001) were independent prognostic factors for DFS as identified by multivariate Cox regression analysis. PLN + PAN adenectomy significantly improved DFS compared to PLN adenectomy alone in patients with noninfiltrating type (P = 0.001), but not in patients with infiltrating type (P = 0.075). PLN + PAN adenectomy significantly improved DFS compared to PLN adenectomy alone in patients with 0 or 1 positive PLN site (P = 0.001, P = 0.009 respectively), but not in patients with ≥2 positive PLN sites (P = 0.095). In the N1 and N2 stage groups, PLN + PAN adenectomy significantly improved DFS compared with PLN adenectomy alone (P = 0.001; P < 0.0001, respectively). Furthermore, mean DFS was longer in the absence of PAN metastasis (P < 0.0001). PAN metastases appear to be associated with reduced DFS. Total PAN adenectomy may improve DFS in patients with noninfiltrating type, stage III rectal cancer below the peritoneal reflection, who have <2 positive PLN sites.


Assuntos
Adenocarcinoma/cirurgia , Aorta/cirurgia , Excisão de Linfonodo , Neoplasias Retais/cirurgia , Adenocarcinoma/mortalidade , Adenocarcinoma/patologia , Adulto , Idoso , Intervalo Livre de Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Pelve/cirurgia , Peritônio/patologia , Peritônio/cirurgia , Prognóstico , Neoplasias Retais/mortalidade , Neoplasias Retais/patologia , Análise de Regressão , Estudos Retrospectivos
16.
Artigo em Chinês | MEDLINE | ID: mdl-21826903

RESUMO

Giardia lamblia is an important human pathogen that causes diarrhea and malnutrition. As a late-branching eukaryote, G. lamblia may have special mechanisms for regulating gene expression which differ from other eukaryotes. In this paper, the mechanisms that governing regulation of G. lamblia gene expression, such as promoters, transcription factors, transcriptional regulation, translation initiation and epigenetic mechanisms are summarized.


Assuntos
Regulação da Expressão Gênica , Giardia lamblia/genética , Sequência de Bases , Expressão Gênica , Proteínas de Protozoários/genética
17.
J Neurochem ; 116(2): 217-26, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21054390

RESUMO

Astrogliosis occurs after brain ischemia, and excessive astrogliosis can devastate the neuronal recovery. Previous reports show that galectin-1 (Gal-1) regulates proliferation of several cell types and plays an important role after nervous system injuries. Here, we found that expression of Gal-1 was remarkably up-regulated in activated astrocytes around ischemic infarct. Furthermore, under ischemic conditions either in vitro or in vivo, Gal-1 was found to inhibit the proliferation of astrocytes in a dose-dependent manner, attenuate astrogliosis and down-regulate the astrogliosis associated expression of nitric oxide synthase and interleukin-1ß after the ischemia. All these changes were blocked by lactose, suggesting a lectin dependent manner of Gal-1's function. Moreover, 7-day Gal-1 treatment reduced apoptosis of neurons, decreased brain infarction volume and improved neurological function induced by the ischemia. Together, these findings indicate that through reducing astrogliosis related damages, Gal-1 is a potential therapeutical target for attenuating neuronal damage and promoting recovery of brain ischemia.


Assuntos
Astrócitos/metabolismo , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/metabolismo , Galectina 1/fisiologia , Galectina 1/uso terapêutico , Gliose/tratamento farmacológico , Gliose/metabolismo , Recuperação de Função Fisiológica/fisiologia , Animais , Astrócitos/patologia , Células Cultivadas , Modelos Animais de Doenças , Galectina 1/biossíntese , Gliose/patologia , Masculino , Distribuição Aleatória , Ratos , Ratos Wistar , Regulação para Cima/fisiologia
18.
Water Sci Technol ; 62(5): 1060-6, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20818046

RESUMO

This study strived to improve the photocatalytic activity by using liquid-phase non-thermal plasma (LPNTP) technology for preparing N-doping TiO(2) as well as to separate/recover the N-dope TiO(2) particles by using ceramic ultrafiltration membrane process. The yellow color N-doped TiO(2) photocatalysts, obtained through the LPNTP process, were characterized with UV-Vis spectroscopy, X-ray diffraction (XRD), and electron spectroscopy for chemical analysis (ESCA). The UV-Vis spectrum of N-doped TiO(2) showed that the absorption band was shifted to 439 nm and the band gap was reduced to 2.82 eV. The structure analysis of XRD spectra showed that the peak positions and the crystal structure remained unchanged as anatase after plasma-treating at 13.5 W for 40 min. The photocatalytic activity of N-doped TiO(2) was evaluated by azo dyes under visible light, and 63% of them was degraded after 16 hours in a continuous-flow photocatalytic system. For membrane separation/recover system, the recovery efficiency reached 99.5% after the ultrafiltration had been carried out for 90 min, and the result indicated that the photocatalyst was able to be separated/recovered completely.


Assuntos
Compostos Azo/química , Cerâmica , Corantes/química , Nitrogênio/química , Titânio/química , Poluentes Químicos da Água/química , Catálise , Fotólise , Fatores de Tempo , Movimentos da Água , Purificação da Água/instrumentação , Purificação da Água/métodos
19.
Neurochem Res ; 35(11): 1716-24, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20689988

RESUMO

Galectin-1, an endogenous mammalian lectin, has been implicated in a variety of CNS disorders. However, its role in cerebral ischemia is still elusive. In the present study, we investigated the effect of recombinant galectin-1 on production of astrocytic brain-derived neurotrophic factor (BDNF) and functional recovery following ischemia. Endogenous galectin-1 was found to be markedly upregulated, paralleled with increased astrocytic BDNF production under ischemic conditions both in vitro and in vivo. Administration of galectin-1 significantly enhanced the expression and secretion of astrocytic BDNF in dose dependent manner. Moreover, rats subjected to photochemical cerebral ischemia showed reduced neuronal apoptosis in ischemic boundary zone and improved functional recovery after brain infusion of galectin-1 (1 µg/days, 7 days). These results suggest that induction of BDNF in astrocytes by galectin-1 may be a promising intervention to attenuate brain damage after stroke.


Assuntos
Astrócitos/metabolismo , Isquemia Encefálica/fisiopatologia , Fator Neurotrófico Derivado do Encéfalo/biossíntese , Galectina 1/fisiologia , Animais , Astrócitos/efeitos dos fármacos , Galectina 1/farmacologia , Masculino , Camundongos , Ratos , Ratos Wistar , Regulação para Cima
20.
Am J Hypertens ; 22(6): 680-6, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19300423

RESUMO

BACKGROUND: Conjugated linoleic acid (CLA) refers to a group of positional and geometrical conjugated dienoic isomers of linoleic acid. Our aim was to investigate the effect of 8-week dietary CLA supplementation on blood pressure, concentrations of plasma adiponecin, leptin, and as well as angiotensin-converting enzyme (ACE) activity in obese hypertensive subjects. METHODS: Eighty obese individuals with stage 1 uncontrolled essential hypertension were randomized in a double-blind, placebo-controlled trial. Participants were randomized to a daily dose of 4.5 g/day CLA (nine 0.5-g capsules; a 50:50 isomer blend of c 9,t 11 and t 10,c 12 CLA) with 37.5 mg/day ramipril (group 1) or placebo with 37.5 mg/day ramipril (group 2) for 8 weeks. Baseline and endpoint systolic BP, diastolic BP, and concentrations of plasma adiponecin, leptin, angiotensinogen, and ACE activity were measured. RESULTS: Treatment with CLA significantly enhanced the reduction effect of ramipril on systolic BP and diastolic BP (P < 0.05). It also increased plasma adiponectin concentration (P < 0.05) and decreased plasma concentrations of leptin and angiotensinogen (P < 0.05); however, significant change was not observed in ACE activity. CONCLUSIONS: An 8-week long supplementation of CLA enhanced the effect of ramipril on blood pressure reduction in treated obese hypertensive patients. The antihypertensive effect of CLA might be related to the changed secretion of hypertensive adipocytokines in plasma.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/uso terapêutico , Pressão Sanguínea/efeitos dos fármacos , Suplementos Nutricionais , Hipertensão/tratamento farmacológico , Ácidos Linoleicos Conjugados/uso terapêutico , Obesidade/complicações , Ramipril/uso terapêutico , Adiponectina/sangue , Inibidores da Enzima Conversora de Angiotensina/administração & dosagem , Angiotensinogênio/sangue , Pressão Sanguínea/fisiologia , Relação Dose-Resposta a Droga , Método Duplo-Cego , Sinergismo Farmacológico , Feminino , Seguimentos , Humanos , Hipertensão/epidemiologia , Hipertensão/etiologia , Leptina/sangue , Ácidos Linoleicos Conjugados/administração & dosagem , Masculino , Pessoa de Meia-Idade , Obesidade/sangue , Obesidade/epidemiologia , Ramipril/administração & dosagem , Resultado do Tratamento
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