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1.
Artigo em Inglês | MEDLINE | ID: mdl-32078554

RESUMO

This commentary presents a replication study to verify the effectiveness of a sum of squared correlations (SSCOR)-based steady-state visual evoked potentials (SSVEPs) decoding method proposed by Kumar et al.. We implemented the SSCOR-based method in accordance with their descriptions and estimated its classification accuracy using a benchmark SSVEP dataset with cross validation. Our results showed significantly lower classification accuracy compared with the ones reported in Kumar et al.'s study. We further investigated the sources of performance discrepancy by simulating data leakage between training and test datasets. The classification performance of the simulation was remarkable similar to those reported by Kumar et al.. We, therefore, question the validity of evaluation and conclusions drawn in Kumar et al.'s study.

2.
Biochem Pharmacol ; : 113848, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32044354

RESUMO

The enhancement of drug efflux caused by ATP-binding cassette (ABC) transporters (including ABCG2 and ABCB1) overexpression is an important factor for multidrug resistance (MDR) in cancers. After testing the reversal activities of 19 chalcone and bis-chalcone derivatives on MDR cancer cell lines, we found that non-basic chalcone CYB-2 exhibited the most potent reversal activities against both ABCG2- and ABCB1-mediated MDR. The mechanistic studies show that this compound can increase the accumulation of anticancer drugs in both ABCG2- and ABCB1-overexpressing cancer cell lines, resulting from the blocked efflux function of the MDR cancer cell lines. This inhibition is due to the barred ABCG2 and ABCB1 ATPase activities rather than altering the expression or localization of ABCG2 or ABCB1 transporters. The previous studies showed that non-basic chalcones were ABCG2-specific inhibitors; however, we found that non-basic chalcone CYB-2 can be developed as an ABCG2/ABCB1 dual inhibitor to overcome MDR in cancers that co-express both ABCG2 and ABCB1. Moreover, non-basic chalcone CYB-2 has synthetic tractability compared to other chalcone-based derivatives.

3.
J Affect Disord ; 263: 500-506, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31759662

RESUMO

BACKGROUND: Few systemic studies of psychotic depression (PD) have been conducted in first-episode and drug-naïve (FEDN) patients. This study was to examine the prevalence of PD and its clinical correlates in a large sample of FEDN outpatients with major depressive disorder (MDD) in a Chinese Han population, which to our best knowledge has not been studied. METHODS: In this cross-sectional study, a total of 1718 FEDN MDD outpatients were recruited. All patients were rated on the positive symptom subscale of the Positive and Negative Syndrome Scale (PANSS) for psychotic symptoms, the 17-item Hamilton Depression Rating Scale (HAMD-17) for depression and Hamilton Anxiety Rating Scale (HAMA) for anxiety. RESULTS: The prevalence of PD was 10.0% (171/1718) in MDD patients. Compared to the non-PD patients, PD patients had significantly older age, lower educational levels, higher anxiety symptom score and were more likely to attempt suicide. Further logistic regression analysis indicated that PD was associated with older age, suicide attempt and comorbid anxiety (all p < 0.05). Multiple regression analyses showed that both HAMD and HAMA total scores were significantly associated with PANSS positive symptom subscale score. LIMITATIONS: Our cross-sectional study design does not show a direct causal relationship between psychiatric symptoms and related factors in patients with MDD. CONCLUSIONS: Our results suggest a high prevalence of PD in MDD patients in the acute early stage in a Chinese Han population, which is associated with demographic variables and clinical symptoms.

4.
J Neural Eng ; 17(1): 016033, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31747642

RESUMO

OBJECTIVE: In recent years, brain-computer interface (BCI) systems based on electroencephalography (EEG) have developed rapidly. However, the decoding of voluntary finger pre-movements from EEG is still a challenge for BCIs. This study aimed to analyze the pre-movement EEG features in time and frequency domains and design an efficient method to decode the movement-related patterns. APPROACH: In this study, we first investigated the EEG features induced by the intention of left and right finger movements. Specifically, the movement-related cortical potential (MRCP) and event-related desynchronization (ERD) features were extracted using discriminative canonical pattern matching (DCPM) and common spatial patterns (CSP), respectively. Then, the two types of features were classified by two fisher discriminant analysis (FDA) classifiers, respectively. Their decision values were further assembled to facilitate the classification. To verify the validity of the proposed method, a private dataset containing 12 subjects and a public dataset from BCI competition II were used for estimating the classification accuracy. MAIN RESULTS: As a result, for the private dataset, the combination of DCPM and CSP achieved an average accuracy of 80.96%, which was 5.08% higher than the single DCPM method (p  < 0.01) and 10.23% higher than the single CSP method (p  < 0.01). Notably, the highest accuracy could achieve 91.5% for the combination method. The test accuracy of dataset IV of BCI competition II was 90%, which was equal to the best result in the existing literature. SIGNIFICANCE: The results demonstrate the MRCP and ERD features of pre-movements contain significantly discriminative information, which are complementary to each other, and thereby could be well recognized by the proposed combination method of DCPM and CSP. Therefore, this study provides a promising approach for the decoding of pre-movement EEG patterns, which is significant for the development of BCIs.

5.
Food Res Int ; 127: 108628, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31882076

RESUMO

Perfluorodecanoic acid (PFDA) is a highly toxic food contaminant that is extensively used in food applications as surface antifouling agent. In this present study, we aimed to assess whether green tea polyphenols (GTPs) and epigallocatechin-3-gallate (EGCG) exert protective effects against PFDA-induced liver damage and inflammation in mice. A mouse model to evaluate liver toxicity was established by giving mice drinking water containing different concentrations of PFDA. GTPs or EGCG (0.32%, w/v) were co-administered to mice exposed to PFDA in drinking water. Overall, GTPs and EGCG extended the survival time and inhibited weight loss among mice who received a lower dose of PFDA. Moreover, GTPs and EGCG ameliorated hepatic oxidative stress, cell apoptosis, necrosis, steatosis, edema, and degeneration, reduced hepatic inflammation and NLRP3 inflammasome activation caused by a moderate dose of PFDA. Taken together, these results show that GTPs or EGCG (or green tea intake) supplements can be beneficial for people exposed to PFDA.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31831401

RESUMO

Event-related potentials (ERPs) are one of the most popular control signals for brain-computer interfaces (BCIs). However, they are very weak and sensitive to the experimental settings including paradigms, stimulation parameters and even surrounding environments, resulting in a diversity of ERP patterns across different BCI experiments. It's still a challenge to develop a general decoding algorithm that can adapt to the ERP diversities of different BCI datasets with small training sets. This study compared a recently developed algorithm, i.e. discriminative canonical pattern matching (DCPM), with seven ERP-BCI classification methods, i.e. linear discriminant analysis (LDA), stepwise LDA, bayesian LDA, shrinkage LDA, spatial-temporal discriminant analysis (STDA), xDAWN and EEGNet for the single-trial classification of two private EEG datasets and three public EEG datasets with small training sets. The feature ERPs of the five datasets included P300, motion visual evoked potential (mVEP), and miniature asymmetric visual evoked potential (aVEP). Study results showed that the DCPM outperformed other classifiers for all of the tested datasets, suggesting the DCPM is a robust classification algorithm for assessing a wide range of ERP components.

7.
Radiat Oncol ; 14(1): 196, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703705

RESUMO

BACKGROUND: To compare irradiation dose to the second and third portions of duodenum (Duo2 and Duo3) with a new refined and old delineation method of para-aortic region for patients with locally advanced cervical cancer (LACC) receiving prophylactic extended-field radiotherapy (EFRT). METHODS: Twenty consecutive patients with LACC were treated with prophylactic EFRT from January 2016 to January 2017 at our institute. Two delineation methods of para-aortic region were designed for each patient, the old delineation method ensured a full coverage of aortic and inferior vena cava, while the right paracaval region above L3 was omitted from CTV in the new delineation method. Patients received a dose of 50.4Gy in 28 fractions for PCTV and a dose of 60.2Gy in 28 fractions for PGTV with volumetric-modulated arc therapy (VMRT). The dose delivered to Duo2 and Duo3 with these two delineation methods were compared. RESULTS: All treatment plans achieved excellent target volume coverage with 95% of PCTV receiving 50.4Gy and 95% of PGTV receiving 60.2Gy. There was no difference between delineation methods in low dose level (V5, V10, V15, V20, V25) for Duo2 and Duo3. The V30, V35, V40, V45, V50, Dmax, Dmean and D2cc for Duo2 with the new and old delineation methods were 55.76% vs 80.54% (P = 0.009), 34.72% vs 70.91% (P < 0.001), 18.69% vs 55.46% (P < 0.001), 8.20% vs 41.49% (P < 0.001), 1.86% vs 21.60% (P < 0.001), 49.58Gy vs 52.91Gy (P = 0.002), 30.38Gy vs 39.22Gy (P = 0.001) and 37.90Gy vs 48.64Gy (P < 0.001) respectively. For Duo3, the new delineation method achieved significant advantages in V30, V35, V40, V45, V50 and Dmean over the old one (96.82% vs 99.25%, P = 0.021; 89.65% vs 97.21%, P = 0.001; 79.50% vs 93.18%, P < 0.001; 65.63% vs 82.93%, P < 0.001; 43.39% vs 65.60%, P < 0.001; 46.09Gy vs 49.24Gy, P < 0.001), no deference was observed regarding D2cc and Dmax with these two delineation methods. CONCLUSION: With the new delineation method of para-aortic area in prophylactic EFRT, significant reduction of irradiation dose to the second and third portions of duodenum in high dose area was obtained. This may further lower the incidence of duodenal toxicity when performing prophylactic EFRT for patients with LACC.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31714240

RESUMO

Ensemble pruning, selecting a subset of individual learners from an original ensemble, alleviates the deficiencies of ensemble learning on the cost of time and space. Accuracy and diversity serve as two crucial factors, while they usually conflict with each other. To balance both of them, we formalize the ensemble pruning problem as an objection maximization problem based on information entropy. Then we propose an ensemble pruning method, including a centralized version and a distributed version, in which the latter is to speed up the former. Finally, we extract a general distributed framework for ensemble pruning, which can be widely suitable for most of the existing ensemble pruning methods and achieve less time-consuming without much accuracy degradation. Experimental results validate the efficiency of our framework and methods, particularly concerning a remarkable improvement of the execution speed, accompanied by gratifying accuracy performance.

9.
Adv Exp Med Biol ; 1101: 41-65, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31729671

RESUMO

Brain-computer interfaces (BCIs) provide a direct communication channel between human brain and output devices. Due to advantages such as non-invasiveness, ease of use, and low cost, electroencephalography (EEG) is the most popular method for current BCIs. This chapter gives an overview of the current EEG-based BCIs for the main purpose of communication and control. This chapter first provides a taxonomy of the EEG-based BCI systems by categorizing them into three major groups: (1) BCIs based on event-related potentials (ERPs), (2) BCIs based on sensorimotor rhythms, and (3) hybrid BCIs. Next, this chapter describes challenges and potential solutions in developing practical BCI systems toward high communication speed, convenient system use, and low user variation. Then this chapter briefly reviews both medical and non-medical applications of current BCIs. Finally, this chapter concludes with a summary of current stage and future perspectives of the EEG-based BCI technology.


Assuntos
Interfaces Cérebro-Computador , Eletroencefalografia , Comunicação , Potenciais Evocados , Humanos
10.
Acta Pharm Sin B ; 9(5): 871-879, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31649840

RESUMO

RAS is one of the most well-known proto-oncogenes. Its gain-of-function mutations occur in approximately 30% of all human cancers. As the most frequently mutated RAS isoform, KRAS is intensively studied in the past years. Despite its well-recognized importance in cancer malignancy, continuous efforts in the past three decades failed to develop approved therapies for KRAS mutant cancer. KRAS has thus long been considered to be undruggable. Encouragingly, recent studies have aroused renewed interest in the development of KRAS inhibitors either directly towards mutant KRAS or against the crucial steps required for KRAS activation. This review summarizes the most recent progress in the exploration of KRAS-targeted anticancer strategies and hopefully provides useful insights for the field.

12.
Food Res Int ; 125: 108516, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31554085

RESUMO

The main objectives of the study were to compare the phenolic composition, chemical and biological antioxidant activities, and cytotoxicity towards IMR90, HCT8, and A549 cell lines of eight grades of Chinese keemun black tea (Camellia sinensis var. sinensis) using a statistical approach. No cytotoxic effects were observed on IMR90 normal cells. Our results all together show that the chemical antioxidant capacity of high-grade black teas measured by DPPH, FRAP, and total reducing capacity assays was correspondingly higher than the mean values of low-grade teas and these antioxidant assays were not associated with cytotoxicity towards cancerous cell lines (HCT8 and A549). High grades of Chinese keemun black teas contained higher contents of total phenolics, flavonoids and ortho-diphenols than lower grades and theaflavin-3,3'-di-gallate could only be detected in high black tea grades (T1 and T2). Intermediate-high keemun black tea grades - C1, C3, T1, and T2 - which also had the highest mean values of TPC, flavonoids, o-diphenols, theaflavin-3-gallate, theaflavin-3'-gallate, Fe2+ chelating ability, and chemical antioxidant activity, presented the highest inhibition of Wistar rat's brain oxidation. No clear differentiation and trend were observed between erythrocyte protection and Chinese black tea grades as results clearly showed that intermediate black tea grades (C3 and C4) protected more the human erythrocytes against mechanical stress. Our study shows that although higher Chinese keemun black tea grades (T1 and T2) presented the highest TPC, flavonoids, and chemical antioxidant activity, these in vitro chemical assays were not translated into higher biological activity.

13.
Opt Express ; 27(18): 25314-25329, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31510405

RESUMO

Continuous-variable quantum key distribution (CVQKD) provides an approach for secure communication in optical fiber communication systems. However, its practical implementation has been hindered by low secret key bit rates that are usually limited to several bits/s to hundreds of kbits/s at distances of more than 25 kilometers. In this paper, we use a pair of optical frequency combs (OFCs) for both multiple parallel transmission and coherent reception, which assign multiple sub-channels involving multiple independent secret keys in a single fiber to increase the key bit rate. The first and last sub-channels are selected for propagating phase references to compensate the phase offset between two free-running combs. We analyze possible excess noise caused by dispersive walk-off in the transmission, imperfect phase compensation in the reception and photon leakage from the phase references. Compared to the previous single-channel CVQKD method, simulation results show more than a factor of 20 increase in the secret key rate at a transmission distance of 35 km and the number of comb lines of 35.

14.
Eur J Med Chem ; 179: 849-862, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31302589

RESUMO

Ko143, a potent ABCG2 inhibitor that reverses multidrug resistance in cancer, cannot be used clinically due to its unsuitable metabolic stability. We identified benzoyl indoles as reversal agents that reversed ABCG2-mediated multidrug resistance (MDR), with synthetic tractability and enhanced metabolic stability compared to Ko143. Bisbenzoyl indole 2 and monobenzoyl indole 8 significantly increased the accumulation of mitoxantrone (MX) in ABCG2-overexpressing NCI-H460/MX20 cells, and sensitized NCI-H460/MX20 cells to mitoxantrone. Mechanistic studies were conducted by [3H]-MX accumulation assay, Western blot analysis, immunofluorescence analysis and ABCG2 ATPase assay. The results revealed that the reversal efficacies of compounds 2 and 8 were not due to an alteration in the expression level or localization of ABCG2 in ABCG2-overexpressing cell lines. Instead, compounds 2 and 8 significantly stimulated the ATP hydrolysis of ABCG2 transporter, suggesting that these compounds could be competitive substrates of ABCG2 transporter. Overall, the results of our study indicated that compounds 2 and 8 significantly reversed ABCG2-mediated MDR by blocking the efflux of anticancer drugs.


Assuntos
Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores , Antineoplásicos/farmacologia , Dicetopiperazinas/farmacologia , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia , Proteínas de Neoplasias/antagonistas & inibidores , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo , Antineoplásicos/química , Antineoplásicos/metabolismo , Dicetopiperazinas/química , Dicetopiperazinas/metabolismo , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Compostos Heterocíclicos de 4 ou mais Anéis/química , Compostos Heterocíclicos de 4 ou mais Anéis/metabolismo , Humanos , Estrutura Molecular , Proteínas de Neoplasias/metabolismo , Relação Estrutura-Atividade , Células Tumorais Cultivadas
15.
Rev Sci Instrum ; 90(5): 055004, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31153264

RESUMO

In order to improve the applicability of piezoelectric actuators (PEAs) in precision positioning, least squares support vector regression (LS-SVR) is applied to model hysteresis in PEAs due to its high modeling accuracy and fast convergence speed. However, low robustness of LS-SVR makes modeling accuracy susceptible to noises, which makes LS-SVR hysteresis models difficult to be applied in engineering environment. In this article, a robust truncated least squares support vector regression (T-LSSVR) is proposed. With the truncation strategy, redundancy in the training set is reduced and robustness is improved. Parameters required for T-LSSVR are optimized by particle swarm optimization and cross optimization algorithms. To test the proposed approach, it is applied to predict the hysteresis of PEAs. Results show that the proposed method is more accurate and robust than other versions of LS-SVR when the training set is polluted by noises, and meanwhile reduces the sample size and increases computational efficiency.

16.
Food Chem ; 297: 124950, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253304

RESUMO

Leaves of plants from the genus Camellia (CAM) are used to make tea; however, there are limited data that compares chemical composition and biological activity of CAM cultivars used to make six tea types. Fourteen CAM cultivars were analyzed by HPLC and UPLC-Q-TOF-MS/MS and biological activity was assessed in a cell growth assay. Tea bioactives and cell growth inhibition varied 2-4 fold. EGCG was the dominant catechin that predicted the magnitude of growth inhibition. However, pure EGCG did not fully account for inhibitory activity suggesting that it may serve as a chemical marker for bioefficacy. As an unbiased characterization of differences in chemical composition among CAM, individual metabolomes were determined and used to generate principle components (PC). PC's from the metabolome were complementary to those from targeted analyses of tea bioactives and were predictive of growth inhibition. This study provides a frame work for identifying CAM cultivars with beneficial traits.


Assuntos
Camellia sinensis/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Camellia/química , Catequina/análogos & derivados , Catequina/análise , Catequina/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Análise de Alimentos/métodos , Análise de Alimentos/estatística & dados numéricos , Humanos , Metaboloma , Extratos Vegetais/química , Análise de Componente Principal , Espectrometria de Massas em Tandem , Chá/química
17.
Food Chem ; 297: 124963, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253305

RESUMO

Authentication of ground coffee has become an important issue because of fraudulent activities in the sector. In the current work, sixty-seven Brazilian coffees produced in different geographical origins using organic (ORG, n = 25) and conventional (CONV, n = 42) systems were analyzed for their stable isotope ratios (δ13C, δ18O, δ2H, and δ15N). Data were analyzed by inferential analysis to compare the factors whereas linear discriminant analysis (LDA), k-nearest neighbors (k-NN), and support vector machines (SVM) were used to classify the coffees based on their origin. ORG and CONV cultivated coffees could not be differentiated according to C stable isotope ratio (δ13C; p = 0.204), but ORG coffees presented higher values of the N stable isotope ratio (δ15N; p = 0.0006). k-NN presented the best classification results for both ORG and CONV coffees (87% and 67%, respectively). SVM correctly classified coffees produced in São Paulo (75% accuracy), while LDA correctly classified 71% of coffees produced in Minas Gerais.


Assuntos
Café/química , Análise de Alimentos/métodos , Espectrometria de Massas/métodos , Brasil , Isótopos de Carbono/análise , Deutério/análise , Análise Discriminante , Análise de Alimentos/estatística & dados numéricos , Espectrometria de Massas/estatística & dados numéricos , Isótopos de Nitrogênio/análise , Agricultura Orgânica , Isótopos de Oxigênio/análise , Máquina de Vetores de Suporte
18.
Environ Technol ; : 1-14, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31158062

RESUMO

In this work, using an agricultural waste of sugarcane bagasse, new biomass of magnetic sugarcane bagasse activated carbon (MSBAC) has been successfully prepared by a simple microwave method. The composition and structure of MSBAC were characterised by SEM, XRD, BET, and FT-IR. It was found that MSBAC was a mesoporous material with a loose structure and rough surface, and it had a high specific surface area. The pHPZC was 4.1, and MSBAC presented a greater amount of acid functional groups than basic groups, making it efficient for adsorption of cationic dye. To study the adsorption ability of MSBAC, methylene blue (MB) was selected as sample pollutant. Effects of pH, MSBAC dosage, initial MB concentration, temperature, time on the adsorption of MB, and the possibility of regeneration of MSBAC were investigated. The adsorption results showed that the maximum adsorption capacity was 36.14 mg·g-1, and the pH had no significant effect on the MB adsorption in the range of 2-10. The equilibrium data fitted Langmuir isotherm, and the adsorption kinetic data obeyed pseudo-second-order kinetic model. The adsorption process involving the surface diffusion and film diffusion. The positive value of ΔH revealed the adsorption behaviour was an endothermic process. The salt concentration had a negative effect on MB removal. MSBAC had a good magnetic separation performance. The used MSBAC could be regenerated by a simple calcination method under the temperature of 300℃ for 30 min.

19.
Biomed Pharmacother ; 116: 109025, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31154267

RESUMO

Rheumatoid arthritis (RA) is a chronic, inflammatory, synovitis-dominated systemic disease with unknown etiology. RA is characterized by the involvement of multiple affected joints, symmetry, and invasive arthritis of the limbs, which can lead to joint deformity, cartilage destruction, and loss of function. Cannabinoid receptor 2 (CB2) has potent immunomodulatory and anti-inflammatory effects and is predominantly expressed in non-neuronal tissues. In the current study, the role of CB2 in the process of inflammatory bone erosion in RA was examined. The selective agonist or high-affinity ligand of CB2 (4-quinolone-3-carboxamides CB2 agonist, 4Q3C CB2 agonist, 4Q3C) significantly reduced the severity of arthritis, decreased histopathological findings, and markedly reduced bone erosion in collagen-induced arthritis (CIA) mice. In addition, 4Q3C prevented an increase in the nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) ratio and inhibited the formation of osteoclasts in CIA mice. Furthermore, the expression of tumor necrosis factor-alpha, interleukin-1ß, cyclooxygenase-2, and inducible nitric oxide synthase was lower in 4Q3C-treated CIA mice than in control CIA mice. Micro-computed tomography corroborated the finding that 4Q3C reduced joint destruction. These data clearly indicate that the CB2-selective agonist, 4Q3C, may have anti-inflammatory and anti-osteoclastogenesis effects in RA and may be considered to be a novel treatment for RA.


Assuntos
Artrite Experimental/tratamento farmacológico , Inflamação/tratamento farmacológico , Articulações/patologia , Substâncias Protetoras/uso terapêutico , Receptor CB2 de Canabinoide/agonistas , Animais , Artrite Experimental/complicações , Artrite Experimental/diagnóstico por imagem , Artrite Experimental/patologia , Biomarcadores/metabolismo , Osso e Ossos/patologia , Citocinas/sangue , Inflamação/complicações , Inflamação/diagnóstico por imagem , Inflamação/patologia , Mediadores da Inflamação/sangue , Articulações/diagnóstico por imagem , Masculino , Camundongos Endogâmicos DBA , Osteoprotegerina/metabolismo , Ligante RANK/metabolismo , Receptor CB2 de Canabinoide/metabolismo , Microtomografia por Raio-X
20.
Food Funct ; 10(7): 3898-3908, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31187838

RESUMO

Inflammatory liver diseases present a significant public health problem. Green tea polyphenols (GTPs) have a myriad of health benefits in animals and humans, including alleviating of hepatic inflammation; however, the underlying mechanisms are complicated and remain unclear. The current study investigated the preventive effects and mechanism of GTPs on lipopolysaccharide (LPS)-induced inflammatory liver injury in mice. The ICR mice received intragastric GTPs once per day for 7 consecutive days prior to LPS stimulation (15 mg kg-1, intraperitoneally) and liver damage and oxidative stress, pro-inflammatory cytokines, and the hepatic nuclear factor-κB (NF-κB) and Nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasomes were observed. Our results showed that GTP supplementation significantly reduced LPS-induced plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and hepatic malondialdehyde (MDA) levels; and LPS-induced reduction of glutathione (GSH) levels and total superoxide dismutase (T-SOD) activities was drastically improved by GTP pretreatment. GTP supplementation significantly reduced plasma contents and hepatic mRNA levels of interleukin (IL)-1ß, IL-18, IL-6, and tumor necrosis factor (TNF)-α, compared with LPS-treated mice which did not receive GTP treatment. In addition, the production of cytokines, such as IL-1ß, IL-18, IL-6, and TNF-α in mice livers, and acute-phase response (plasma levels of nitric oxide and C-reactive protein) were also decreased following GTP pre-treatment. Furthermore, GTPs reduced LPS-induced hepatic NF-κB signaling and NLRP3 inflammasome activation. GTPs exert protective effects against inflammatory liver injury by regulating NF-κB signaling and the NLRP3 inflammasome activation. Our findings suggest that dietary GTP supplementation may be an adjunctive prevention and treatment for acute liver injury-associated inflammation.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Inflamassomos/antagonistas & inibidores , Lipopolissacarídeos/efeitos adversos , Proteína 3 que Contém Domínio de Pirina da Família NLR/antagonistas & inibidores , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Chá/química , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Citocinas/metabolismo , Modelos Animais de Doenças , Glutationa/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Malondialdeído , Camundongos , Camundongos Endogâmicos ICR , NF-kappa B/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Superóxido Dismutase
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