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1.
Mater Sci Eng C Mater Biol Appl ; 120: 111672, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33545837

RESUMO

The application of digitally manufactured dental metals has aroused the attention on their biocompatibilities. Three-dimensional oral mucosal model (3D OMM) would provide excellent assessments to the biocompatibility. In the current study, we set to measure metal ion release levels in the extracts of cast gold-platinum alloy (Au-Pt), differently manufactured cobalt-chromium alloy (Co-Cr) and commercially pure titanium (cp-Ti). We further tested two scaffold materials of 3D OMM to determine the better one for the succedent work. Lastly, we evaluated the apoptotic and autophagic effects of cast Au-Pt, and differently manufactured Co-Cr and cp-Ti on mucosal cells based on 3D OMM. We found that, in the construction of 3D OMM, Matrigel showed better performance than bovine acellular dermal matrix. Thus, Matrigel was chosen to construct the 3D OMM in the succedent studies. The results of ion release and biological assessments showed that, firstly, cast Au-Pt and cp-Ti triggered less early apoptotic cells and ion release than cast Co-Cr, implying better chemical stability and biocompatibility of them; secondly, digitally manufactured (including CAD/CAM milling and SLM) Co-Cr showed significantly lower ion release levels and lesser early apoptotic effects on 3D OMM as compared to the cast one. Although cast cp-Ti released much more ions than CAD/CAM milling one, manufacturing methods had no impact on apoptotic effect of cp-Ti. Therefore, we believe that digital methods possess same or even better chemical stability and biocompatibility than conventional casting one. Thirdly, although increased autophagic levels are observed in all test groups, so far there is no evidence that the test metals trigger different levels of autophagy as compared to each other. In addition, correlation analysis indicates that Co, W, and Mn appear to be the potential inducements for the apoptotic and autophagic effects of Co-Cr.

2.
J Hazard Mater ; 412: 125214, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33529835

RESUMO

Our previous study revealed PAHs' wide occurrence in corals from multiple coral reef regions (CRRs) in the South China Sea. However, little is known about their occurrence, distribution, fate, and sources in the ambient environment of these CRRs. This study aimed to resolve these research gaps. The results showed ∑15PAHs (total concentrations of 15 US EPA priority controlled PAHs exclude naphthalene) in the atmosphere (gas-phase: 0.31-49.6 ng m-3; particle-phase: 2.6-649 pg m-3) were mainly influenced by air mass origins. Southwesterly wind caused higher ∑15PAHs than the southeasterly wind. The ∑15PAHs in seawater from the nearshore (462 ± 244 ng L-1) was higher than that from offshore Zhongsha Islands (80.5 ± 72.1 ng L-1) because of the effect of terrigenous pollution and ocean current. Source apportionment indicated that the mixed sources of spilled oil and combustion from neighboring countries were the main contributors to PAHs in these CRRs. The total deposition fluxes showed that PAHs tended to migrate from the atmosphere to seawater. Global warming may inhibit this process, but PAHs still have a migration pattern of atmosphere-ocean-corals, which will further increase the environmental pressure on coral reef ecology.

3.
Diabetes Res Clin Pract ; : 108702, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33609619

RESUMO

AIMS: This research aimed to investigate the effects of high glucose(HG) on the innate immunity of podocytes and diabetic nephropathy (DN) mice via Toll like receptor (TLR) signaling, and explore the protective effectsof human umbilical cord mesenchymal stem cells (HUC-MSCs) on this process. METHODS: HUC-MSCs obtained from human umbilical cord were cocultured with podocytes and transplanted into DN mice. Flow cytometry, CCK-8assay, ELISA, western blot analysis, periodicacid-schiff, masson, immunohistochemistry and immunofluorescence staining was used to detect the inflammation, TLR signaling, physical, biochemical and morphological parameters in podocytes and DN mice. RESULTS: HG reduced the viability of podocytes, activated TLR2 and TLR4 signaling pathway and increased the expression of inflammatory cytokines such as IL-6, IL-1ß, TNF-α, and MCP-1 in podocytes and DN mice. However, HUC-MSCs decreased the inflammation and restrained the TLR signaling pathway caused by HG in vitro and in vivo. Furthermore the rhHGF decreased the expression of TLR2 and TLR4 while the blockade of HGF increased the expression of TLR2 and TLR4 in podocytes. CONCLUSIONS: HUC-MSCs have benefits to the podocytes under HG and the progression of DN by inhibiting TLR signaling pathway and depressing the inflammation. HUC-MSCs may be a therapeutic strategy for treating patients with DN.

4.
J Phys Chem Lett ; : 861-868, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33428415

RESUMO

The hot carrier cooling dynamics in the C-excitonic state of monolayer MoS2 is slowed down by the hot phonon bottleneck and Auger heating effects, as exploited by ultrafast transient absorption spectroscopy. The hot carrier cooling process, determined by the hot phonon bottleneck, can be prolonged through rising the excitation photon energy or increasing the absorbed photon flux. By inducing the Auger heating effect under higher absorbed photon flux, the hot carrier lifetime also increases at the low excitation photon energy. When these two effects are combined under higher excitation photon energy and higher absorbed photon flux, the hot phonon bottleneck is gradually weakened because of Auger recombination. In addition, the similar hot carrier phenomenon can be observed in A/B excitonic states owing to the same physical mechanism. Our work establishes a solid photophysics foundation for 2D transition-metal dichalcogenide applications in advanced energy conversion, optical quantum communication, quantum technology, etc.

5.
Anal Methods ; 13(5): 672-684, 2021 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-33475104

RESUMO

A novel magnetic molybdenum disulfide@graphene (Fe3O4/MoS2@G) nanocomposite with amphiphilic properties was prepared via a co-mixing solvothermal method. To demonstrate the feasibility of Fe3O4/MoS2@G as a sorbent during sample preparation, it was employed for the magnetic solid phase extraction (MSPE) of ten pyrethroids, three triazoles and two acaricide pyridaben and picoxystrobin in an emulsified aqueous solution. Dichloromethane was used as the extractant to form an emulsified aqueous solution. Subsequently, the Fe3O4/MoS2@G sorbent with amphiphilic properties was used to retrieve 15 wide polarity insecticides from dichloromethane via MSPE. The proposed method has the advantage of being applicable to different polar pesticides, strengthening the capacity of enrichment and purification of target analytes. The π-π interaction between the hydrophilic and hydrophobic moieties of Fe3O4/MoS2@G and the aromatic rings of target analytes were responsible for the efficient sorption. Thus, a reliable, convenient, and efficient method for the analysis of 15 insecticides with wide polarity in wolfberry samples was established by coupling Fe3O4/MoS2@G nanocomposite MSPE with gas chromatography-mass spectrometry (GC-MS) analysis. The obtained linearity of this method was in the range from 1 to 5000 ng mL-1 for 15 analytes, with determination coefficients (R2) ≥0.9907. The limit of detection (LOD) for 15 insecticides was in the range from 0.1 to 5.0 ng g-1. The recoveries of 15 insecticides from spiked wolfberry samples were in the range from 71.41% to 110.53%, and RSD was less than 14.8%.

6.
Nanomaterials (Basel) ; 11(2)2021 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-33513842

RESUMO

In this paper, we explore whether interparticle spacing affects steady-state and transient-state optical properties by comparing close-packed CdSe/ZnS-quantum dots (QDs) and CdSe/ZnS-QDs dispersed in polymethyl methacrylate (PMMA). High-pressure is an effective physical means to adjust the interparticle spacing of QDs, which may artificially expand the application of QDs further. The results under high-pressure indicate that it is the reduced interparticle spacing rather than the enhanced quantum confinement effect with volume compression that has a stronger effect on exciton relaxation of CdSe/ZnS-QDs. This work is hoped to help us further understand the effect of interparticle spacing among QDs in various integrated environments.

7.
J Hazard Mater ; 403: 123618, 2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32823029

RESUMO

The usage of alternative per- and polyfluoroalkyl substances (PFASs) has been increasing due to the restriction and elimination of legacy PFASs. However, there is limited knowledge on bioaccumulation and trophic magnification of alternative PFASs, especially in subtropical ecosystems. In the present study, we performed a comprehensive survey to investigate the occurrence, bioaccumulation and trophic magnification of legacy and alternative PFASs in subtropical marine food webs in the Beibu Gulf, South China. Results showed that perfluorobutanoic acid (PFBA) and perfluorooctanoic acid (PFOA) were the predominant PFASs in water phase, while perfluorooctane sufonate (PFOS) contributed most to the sum of target PFASs in sediments and marine organisms. Of the investigated PFASs, PFOS and 6:2 chlorinated polyfluoroalkyl ether sulfonic acids (F-53B) exhibited the highest bioaccumulation factor with values > 5000, qualifying as very bioaccumulative chemicals. There was a significant positive correlation between log BSAF and the carbon chain length of perfluoroalkyl carboxylic acids (PFCAs). Trophic magnification (TMF) was observed for PFOS and F-53B, while the remaining PFASs were biodiluted through the present food web. The hazard ratios for PFOS and PFOA in all organisms were far less than unity, suggesting overall low PFAS risks for humans through consumption of marine organisms.

8.
Sci Total Environ ; 752: 141882, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32889286

RESUMO

Antibiotic residues in mariculture environments have been detected globally, while little information is available about their dynamic levels, source, behavior, and fate during the whole culture process. In this study, the dynamic occurrence, bioaccumulation, source, fate, and human dietary risk of 19 antibiotics were investigated in different breeding stages of mariculture ponds near the Maowei Sea, South China. Fourteen antibiotics, including three sulfonamides (SAs), five fluoroquinolones (FQs), three macrolides (MLs), and two chloramphenicols (CAPs), were detected in the mariculture ponds, with FQs being the most abundant antibiotics. Significant variations of antibiotic concentration occurred during the whole culture process. Severe weather, especially typhoons and rainstorms, resulted in the average highest levels of ∑19antibiotics (mean: 567 ng L-1) in mariculture ponds. The source apportionment estimated for the mariculture ponds showed that direct application was the primary source of antibiotics (91.2%). The antibiotics in mariculture ponds were mainly discharged through aquaculture wastewater (65.8%) and settling particles (33.8%). The estimated annual input of antibiotics into the Maowei Sea was 2.24 times higher through the two main rivers (48.0 kg a-1) than through the mariculture wastewater (24.1 kg a-1). The apparent bioaccumulation factors (ABAFs) confirmed that young and adult tilapia accumulated more sulfamethoxazole (SMX) and norfloxacin (NOX), respectively. The result from the estimated daily intakes suggested that the antibiotics in the seafood could not pose a risk to human health by dietary exposure assessment. CAPSULE: Big variation of antibiotic concentration occurred during the whole culture process in the mariculture farms, and the storm increased antibiotic application.


Assuntos
Tanques , Poluentes Químicos da Água , Antibacterianos/análise , China , Monitoramento Ambiental , Humanos , Poluentes Químicos da Água/análise
9.
Sci Total Environ ; 751: 141718, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32889462

RESUMO

Antibiotics are of particular concern because of their ubiquity in aquatic environment and long-term adverse effects on aquatic organisms and humans. However, there is no information about the bioaccumulation and trophic magnification of antibiotics in subtropical environments. In this study, we determined the concentrations of 22 antibiotics to investigate their occurrence, bioaccumulation and trophic magnification in a subtropical food web from the Beibu Gulf. The total concentrations of target antibiotics ranged from 52.94-77.76 ng/L in seawater, 9.69-15.43 ng/g dry weight (dw) in sediment, and 0.68-4.75 ng/g wet weight (ww) in marine organisms, respectively. Macrolides were the predominant antibiotics in water, while fluoroquinolones were more abundant in sediment and biota samples. The total concentrations of target antibiotics in examined marine taxa descended in the order: crustacean > cephalopod > fish, with antibiotic profiles displaying distinct difference among taxa. Log BAFs (bioaccumulation factor) for antibiotics in all organisms ranged from -0.50 for erythromycin-H2O (ETM-H2O) to 2.82 for sulfamonomethoxine (SMM). Significantly negative correlation was observed between the log Dow and log BAF values (p < .05), indicating that log Dow is a good predictor of antibiotics bioaccumulation potential in marine organisms. The trophic magnification factors (TMFs) for sulfadiazine (SDZ) and enoxacin (ENX) were greater than unity, suggesting the trophic magnification of these chemicals through the food web. In contrast, enrofloxacin (ENR), ciprofloxacin (CIX), ofloxacin (OFX), norfloxacin (NOX), ETM-H2O and trimethoprim (TMP) were biodiluted in the food web from the Beibu Gulf. This study provides substantial information on the fate and trophic transfer of antibiotics in a subtropical marine ecosystem.


Assuntos
Cadeia Alimentar , Poluentes Químicos da Água , Animais , Antibacterianos/análise , Bioacumulação , China , Ecossistema , Monitoramento Ambiental , Peixes , Humanos , Poluentes Químicos da Água/análise
10.
J Am Chem Soc ; 142(52): 21751-21757, 2020 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-33337859

RESUMO

Although more attention has been attracted to the therapy based on reactive oxygen species (ROS) for tumor therapy in recent years, such as photodynamic therapy and chemodynamic therapy, the limited ROS production rate leads to their poor treatment effect owing to the relatively low content of O2 and H2O2 in tumor microenvironments, confined light penetration depth, strict Fenton reaction conditions (pH 3-4), and so on. Therefore, it is urgent to explore the new agents with highly efficient ROS generation capacity. Herein, we first prepared phospholipid coated Na2S2O8 nanoparticles (PNSO NPs) as new ROS generation agents for in situ generating Na+ and S2O82- through gradual degradation, which can then be changed to toxic •SO4- (a novel reported ROS) and •OH regardless of the amount of H2O2 and pH value in the tumor microenvironment (TME). As the generation of a large amount of Na+, PNSO NPs can bypass the ion transport rules of cells through endocytosis to deliver large amounts of Na+ into the cells, resulting in a surge of osmolarity and rapid cell rupture and lysis. Osmotic pressure induced by PNSO NPs will further lead to an unusual manner of cell death: caspase-1-related pyroptosis. Moreover, all of above effects will cause high immunogenic cell death, regulate the immunosuppressed TME, and then activate systemic antitumor immune responses to combat tumor metastasis and recurrence. We believe PNSO NPs will be new and potential ROS generation agents, and this work will broaden the thinking of the exploring of new antitumor nanodrugs.

11.
Int J Mol Med ; 46(6): 2035-2046, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33125088

RESUMO

Liver fibrosis (LF) is a healing response to wounds resulting in liver injury that can cause liver failure or even cancer without functional prevention. Resveratrol (RSV) has been suggested to exert biological effects against various human diseases. MicroRNA­20a (miRNA/miR­20a) has been shown to promote disease progression. The present study aimed to assess the mechanisms through which RSV induces autophagy and activates the miR­20a­mediated phosphatase and tensin homolog (PTEN)/PI3K/AKT signaling pathway in LF. First, a rat model of carbon tetrachloride (CCL4)­induced LF and a cell model of platelet­derived growth factor (PDGF)­BB­stimulated HSC­T6 cells were established for use in subsequent experiments. Subsequently, RSV at a range of concentrations was injected into the model rats with LF. Indicators related to liver injury, oxidative stress and fibrosis were determined in the rats with LF. The RSV­treated HSC­T6 cells were subjected to transfection with miR­20a mimic and PTEN overexpression plasmid to assess the levels of liver injury and LF. A dual­luciferase reporter gene assay was performed to verify the binding sites between PTEN and miR­20a. RSV was found to alleviate LF in rats, and autophagy was enhanced in the rats with LF following RSV treatment. Furthermore, the activation of the PTEN/PI3K/AKT axis attenuated LF, which was reversed by transfection with miR­20a mimic. RSV reversed the inhibitory effects of miR­20a on PTEN expression, reducing miR­20a expression and promoting PTEN, PI3K and p­AKT protein expression, thus attenuating LF. On the whole, the present study demonstrates that RSV induces autophagy and activates the miR­20a­mediated PTEN/PI3K/AKT signaling pathway to attenuate LF. These findings may lead to the development of potential therapeutic strategies for LF.

12.
Nanoscale Horiz ; 5(12): 1574-1585, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33033819

RESUMO

The defects at the interface and grain boundaries (GBs) of perovskite films limit the performance of perovskite solar cells (PSCs) seriously. Herein, organic semiconductors with different terminal groups including a ladder-type electron-deficient-core-based fused structure (DAD) fused core with 2-(3-oxo-2,3-dihydro-1H-inden-1 ylidene)malononitrile (BTP-4H), DAD with 2-(5,6-dichloro-3-oxo-2,3-dihydro-1H-inden-1 ylidene)malononitrile (BTP-4Cl), and DAD with 2-(5,6-difluoro-3-oxo-2,3-dihydro-1H-inden-1 ylidene)malononitrile (BTP-4F) are introduced into perovskite films to study the effects of the terminal groups on the PSC performance. A physical model is proposed to understand the effects of the terminal groups on the perovskite growth and energy level alignment of devices. Compared with BTP-4H and BTP-4Cl, BTP-4F can more effectively delay the crystallization rate and increase the crystal sizes due to hydrogen bonding of F and FA. BTP-4F can also provide more efficient charge transport channels due to the optimal energy level alignment. Most importantly, BTP-4F can promote charge transport from the perovskite film to spiro-OMeTAD and to SnO2, thus realizing simultaneous up-bottom passivation of perovskite films. Finally, the BTP-4F passivated PSCs exhibit a remarkable PCE of 22.16%, and the device can maintain ∼86% of the initial PCE after 5000 h. Therefore, this work presents significant potential of organic semiconductors in PSCs toward high efficiency and high stability due to the terminal groups.

13.
Int J Syst Evol Microbiol ; 70(11): 5880-5887, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33034551

RESUMO

A novel Gram-stain-negative, non-endospore-forming, motile, and aerobic bacterial strain, M105T, was isolated from coral Porites lutea, and was subjected to a polyphasic taxonomic study. Global alignment based on 16S rRNA gene sequences indicated that M105T shares the highest sequence identity of 94.5 % with Aliikangiella marina GYP-15T. The average nucleotide identity (ANI) and average amino acid identity (AAI) between M105T and A. marina GYP-15T was 69.8 and 71.6 %, respectively. On the basis of the results of phenotypic, chemotaxonomic, phylogenetic, phylogenomic, and comparative genomic analyses, it is concluded that M105T should represent a novel species in the genus Aliikangiella, for which the name Aliikangiella coralliicola sp. nov. is proposed. The type strain is M105T (=MCCC 1K03773T= KCTC 72442T). Furthermore, the family Kangiellaceae was classified into two families on the basis of phylogenetic, phylogenomic, polar lipid profile and motility variations. The novel family Pleioneaceae fam. nov. is proposed to accommodate the genera Aliikangiella and Pleionea.

14.
Int J Syst Evol Microbiol ; 70(11): 5620-5626, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32924922

RESUMO

A novel Gram-stain-negative, non-endospore-forming, non-motile, aerobic bacterium (strain R33T) was isolated from coral Porites lutea and subjected to a polyphasic taxonomic study. The G+C content was 44.5 mol%. The only detected respiratory quinone was menaquinone 6 (MK-6). The major cellular fatty acids were iso-C15 : 0 and iso-C15 : 1 ω6c. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Global alignment based on 16S rRNA gene sequences indicated that strain R33T shares the highest sequence identity of 93.2 % with Muriicola marianensis A6B8T in the family Flavobacteriaceae. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R33T forms a distinct branch in a stable clade comprising strain R33T and members of the genera Muriicola, Robiginitalea, Eudoraea and Zeaxanthinibacter. The phylogenomic analysis also supported this 16S rRNA gene-based phylogenetic result. Comparative genomic analysis indicated that strain R33T is rich in AraC-type DNA-binding domain-containing protein-coding genes, which means the regulation of carbon utilization is very complex. Low 16S rRNA gene identity, different polar lipids and/or cellular fatty acid profiles could readily distinguish strain R33T from any validly published type strains. Therefore, strain R33T is suggested to represent a new species in a new genus, for which the name Poritiphilus flavus gen. nov., sp. nov. is proposed. The type strain is R33T (=MCCC 1K03853T=KCTC 72443T).

15.
Mar Pollut Bull ; 160: 111668, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32927184

RESUMO

A series of flow-through reactor experiments were undertaken to assess the potential effect of porewater advection and dissolved inorganic phosphorus (DIP) concentration on benthic DIP dynamics in permeable sediments collected from the Weizhou Island, northern South China Sea. The flux of DIP ranged from -0.13 to 0.05 mmol m-2 h-1, and the reversal from DIP efflux to influx occurred when the DIP concentration reached a threshold. DIP release from the sediment into the seawater peaked at intermediate advection rate, which perhaps provide optimum conditions for DIP release related to CaCO3 dissolution. Phosphorus limitation in seawater could be relieved by DIP release from the sediment, and CaCO3-bound P in carbonate sands may play a major role in benthic DIP release and decrease in the molar nitrogen/phosphorus ratio in seawater around the Weizhou Island.


Assuntos
Fósforo , Areia , Carbonatos , China , Sedimentos Geológicos , Ilhas , Fósforo/análise
16.
Sci Rep ; 10(1): 15179, 2020 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-32938983

RESUMO

Seventy-five wild tilapia samples from six rivers (ten sites) in Guangxi province were collected and analyzed for 53 organochlorine compounds. DDTs, endosulfan, and PCBs were the most dominant compounds found in this study. Tiandong County (TD) and Guigang City (GG) sites were found to be heavily contaminated with high levels of endosulfan (385-925 ng/g lw) and/or DDTs (20.1-422 ng/g lw). The diagnostic ratios indicated that the residues of DDTs and endosulfan in wild tilapia are associated with historical applications as well as the recent introduction of technical DDTs and endosulfan at some sampling sites. The correlation between total length, body mass, and organochlorines (OCs) was higher than the correlation between age and lipid content. There was no significant correlation between organochlorine pesticides (OCPs) and lipid content. Therefore, for organisms, the feeding intensity (related to length and mass) of fish could better reflect degree of pollution than exposure time (age) of fish. The hazardous ratios for the 50th and 95th percentile data of OCPs and PCBs in fish were both below 1, suggesting that daily exposure to OCPs and PCBs yields a lifetime cancer risk lower than 1 in 10,000.

17.
J Dermatol Sci ; 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32951990

RESUMO

BACKGROUND: Human Apurinic/Apyrimidinic Endonuclease 1 (APE1/REF-1/HAP1) is a multifunction protein involved in the progression of cancer. But the role of APE1 in cutaneous squamous cell carcinoma (cSCC) is unclear. OBJECTIVE: This study is aimed to investigate the basic modulatory mechanism of APE1 in cSCC development and offer a novel potential target for clinical treatment. METHODS: The expression of APE1 in cSCC tissues was detected by western blot and immunohistochemistry (IHC) staining. The function of APE1 and miR-27a in cSCC cells was investigated by cell counting kit-8 (CCK-8) assays, colony formation assays and transwell migration assays. Western blot was used to determine the expression of APE1 in cSCC and epithelial-mesenchymal transition (EMT) markers in HSC-1 and HSC-5 cells with APE1 knockdown or overexpression. Double luciferase reporter assays were performed to confirm the interaction of miR-27a and APE1. RESULTS: We identified that APE1 was significantly upregulated in human cSCC tissues and cSCC cells and its overexpression promoted cell proliferation, migration and the expression of EMT markers in cSCC cells. Mechanistically, miR-27a was predicted and confirmed as the upstream of APE1. Its downregulation also enhanced the proliferation and migration of cSCC cells. Rescue experiments demonstrated that restoration of APE1 expression significantly abolished the inhibition of cell proliferation and migration mediated by miR-27a. CONCLUSION: As a direct gene of miR-27a, APE1 improved cell proliferation and migration to promote the progression of cSCC, which could be considered as a potential therapeutic target for cSCC treatment.

18.
PLoS One ; 15(9): e0239710, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32991592

RESUMO

Early diagnosis is important for the clinical management of diseases caused by dengue virus (DENV) infections. We investigated the performance of three commercially available DENV nonstructural protein 1 (NS1) rapid diagnostic tests (RDTs) using 173 acute-phase sera collected from dengue fever-suspected patients during the 2012-2013 DENV outbreak in Taiwan. The results of the NS1 RDTs were compared with those of qRT-PCR to calculate the sensitivity and specificity of the NS1 RDTs. The anti-DENV IgM and IgG RDT results were included to increase the probability of detecting acute DENV infection. The anti-DENV IgM/IgG RDT results were also compared with those of IgM/IgG captured ELISA. The sera from DENV qRT-PCR-positive patients were subjected to NS1 RDTs, as well as IgM/IgG captured ELISA. These results suggested that there was no significant difference in the sensitivities of the three commercially available DNEV NS1 RDTs; the SD NS1 RDT results showed the highest agreement with the qRT-PCR reference results, followed in order by the Bio-Rad and CTK NS1 RDT results when the specificity was considered. Inclusion of the IgM or IgG RDT results increased the likelihood of diagnosing either a primary or secondary DENV infection. NS1 RDTs were more sensitive for the detection of primary infections than secondary infections, related to DENV viremia levels determined by qRT-PCR. These results suggested that anti-DENV antibodies reduced the sensitivity of NS1 rapid tests. We also analyzed the sensitivity for the detection of different DENV serotypes, and the results suggested that the NS1 RDTs used in this study were valuable for rapid screening of acute DENV infection with DENV-1, DENV-2 and DENV-3. Our results suggest that the NS1 RDT is a good alternative to qRT-PCR analysis for timely dengue disease management and prevention in dengue-endemic regions where medical resources are lacking or during large dengue outbreaks. However, the relatively low sensitivity for DENV-4 might miss the detection of DENV-4-infected cases.

19.
Biomol NMR Assign ; 14(2): 323-327, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32844357

RESUMO

Growth factor receptor-bound 2 (Grb2) is an important link in the receptor tyrosine kinase signaling cascades. It is involved in crucial processes, both physiological (mainly embryogenesis) and pathological (different types of cancer). Several binding partners of all three domains (SH3-SH2-SH3) of this adaptor protein are well described, such as ErbB family members for the SH2 domain and Sos for the SH3 domains. How the different domains interact with each other, both structurally and functionally, is still unclear. These interactions could be essential for regulation processes, and therefore are of great interest. Although a lot of structural data on Grb2 exist, they describe either individual domains, ligand-bound conformations, or frozen pictures of the protein captured by crystallography. Here we report the assignment of backbone and of [Formula: see text] chemical shifts of full-length, apo-Grb2 in solution. In addition to the assigned conformation corresponding to three well-folded domains, a set of peaks compatible with the presence of an unfolded conformation of the N-terminal SH3 domain is observed. This assignment paves the way for future studies of inter-domain interactions and dynamics that have to be taken into account when studying the regulation of Grb2 interactions and signaling pathways.

20.
Environ Monit Assess ; 192(9): 579, 2020 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-32783089

RESUMO

Relatively high-latitude waters are supposed as a refuge for corals under ocean warming. A systematic assessment of the Weizhou Island reef in the northern South China Sea, a relatively high-latitude region, shows that the ecosystem restoration index decreased from 0.96 to 0.62 during the period between 1990 and 2015. Although the biotic community, supporting services, and regulating services remained at good or very good states, the provisioning services, cultural services, and especially habitat structure deteriorated to very poor or moderate states. Gray relational analysis showed that these ecological declines exhibited a strong relationship with human pressures from tourism activities and the petrochemical industry. The recoveries of the biotic community and supporting services that benefited from wintertime warming appeared to be partly offset by intensive human pressures. The long-term effects on ecosystem structure and functions suggest that anthropogenic disturbances have impaired the possibility of this area serving as a potential thermal refuge for reef-building corals in the South China Sea. This study thus provides an integrated approach for assessing the adaptive responses of coral reef ecosystems to climate change and local human activities.


Assuntos
Antozoários , Recifes de Corais , Animais , China , Mudança Climática , Ecossistema , Monitoramento Ambiental , Humanos
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