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1.
Medicine (Baltimore) ; 101(46): e31778, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36401443

RESUMO

Several studies have found associations of genes with atrial fibrillation (AF), including SCN5A-H558R. However, there are limited data of these associations among populations living at different altitudes. We investigated the relationship between the SCN5A-H558R polymorphism and AF in Tibetans living at different altitudes in Qinghai, China. General clinical and genotype data were obtained from 72 patients with AF and 109 non-AF (NAF) individuals at middle altitudes, and from 102 patients with AF and 143 NAF individuals at high altitudes. Multifactor logistic regression was performed to determine associations and AF risk factors. SCN5A-H558R genotypes differed significantly between the AF and NAF groups (P < .0125) and the G allele was an independent AF risk factor (P < .05) at both altitudes, with no significant differences according to altitude (P > .0125). At middle altitudes, age, red blood cell distribution width (RDW-SD), left atrial internal diameter (LAD), and G allele were independent AF risk factors. At high altitudes, age, smoking, hypertension, RDW-SD, free triiodothyronine, LAD, and G allele were independent AF risk factors (P < .05). The G allele of SCN5A-H558R might be an independent risk factor of AF both high and middle altitude, but there are some differences in other clinical risk factors of AF.


Assuntos
Fibrilação Atrial , Humanos , Fibrilação Atrial/epidemiologia , Fibrilação Atrial/genética , Altitude , Tibet/epidemiologia , Fatores de Risco
2.
J Neuroimmunol ; 373: 577999, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36395637

RESUMO

BACKGROUND: Anti-IgLON5 is a rare neurologic disease that can present with epileptic seizures. However, epileptic seizures have not been characterized and are underreported. We aimed to investigate the clinical characteristics and demographics of epileptic seizures in patients with anti-IgLON5 disease. METHODS: We reported a case of anti-IgLON5 disease presenting with epileptic seizures and presented a comprehensive literature review on epileptic seizures in patients with the anti-IgLON5 disease. We searched the Medline, Pubmed, and Web of Science databases using the following search algorithm: "IgLON5" or "anti-IgLON5" or "IgLON5 antibody" limited to publications in English. RESULTS: We identified 183 cases from 66 publications. In addition to our case, nine (4.9%) patients with anti-IgLON5 disease had reported epileptic seizures, either focal or generalized. Of those, epileptic seizures were one of the main reasons for neurology consultation in six (3.2%). Patients with epileptic seizures affected both sexes similarly and usually developed in middle age. In addition to epileptic seizures, a majority of patients had sleep disorders and cognitive impairment. The frequency of epileptic seizures was reduced with the treatment of immunotherapy and antiseizure medication. CONCLUSION: Anti-IgLON5 disease can present with epileptic seizures, and our study expands the clinical spectrum of the anti-IgLON5 disease.


Assuntos
Encefalite , Epilepsia , Doença de Hashimoto , Feminino , Masculino , Pessoa de Meia-Idade , Humanos , Epilepsia/tratamento farmacológico , Epilepsia/epidemiologia , Convulsões , Moléculas de Adesão Celular Neuronais
3.
Nat Commun ; 13(1): 6911, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376324

RESUMO

Precise understanding of interfacial metal-hydrogen interactions, especially under in operando conditions, is crucial to advancing the application of metal catalysts in clean energy technologies. To this end, while Pd-based catalysts are widely utilized for electrochemical hydrogen production and hydrogenation, the interaction of Pd with hydrogen during active electrochemical processes is complex, distinct from most other metals, and yet to be clarified. In this report, the hydrogen surface adsorption and sub-surface absorption (phase transition) features of Pd and its alloy nanocatalysts are identified and quantified under operando electrocatalytic conditions via on-chip electrical transport measurements, and the competitive relationship between electrochemical carbon dioxide reduction (CO2RR) and hydrogen sorption kinetics is investigated. Systematic dynamic and steady-state evaluations reveal the key impacts of local electrolyte environment (such as proton donors with different pKa) on the hydrogen sorption kinetics during CO2RR, which offer additional insights into the electrochemical interfaces and optimization of the catalytic systems.

4.
Chem Sci ; 13(39): 11639-11647, 2022 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-36320394

RESUMO

Aqueous electrochemical nitroarene reduction reaction using H2O as the sustainable hydrogen source is an emerging technology to produce functionalized anilines. However, the development of low-cost electrocatalysts and the fundamental mechanistic understanding of the selective NO-RR still remain challenging. Herein, self-supporting hierarchical nanosheets consisting of high-density Co9S8/Ni3S2 heterojunctions on Ni foam (Co9S8/Ni3S2-NF) are constructed via an in situ self-template strategy. With combined advantages of high-loading, high surface exposure, efficient conductivity and unique electronic structure of the Co9S8/Ni3S2 interface, the as-prepared Co9S8/Ni3S2-NF exhibits efficient electrocatalytic NO-RR performance, including up to 99.0% conversion and 96.0% selectivity towards aniline, and outstanding functional group tolerance. Mechanistic investigations and theoretical calculations reveal that electron transfer from Ni3S2 to Co9S8 is beneficial for the co-adsorption of H2O and nitrobenzene molecules at the interfacial sites, promoting the formation of active hydrogen and subsequent reduction of nitrobenzene. Additionally, the interfacial charge transfer breaks the symmetry of two active Co sites at the Co9S8/Ni3S2 interface, which markedly reduces the energy barrier for reduction of nitrobenzene to aniline. This work offers a successful example for the interfacial engineering of metal sulfide-based heterojunctions with excellent electrocatalytic nitroarene reduction performance, and also paves the way for the in-depth understanding of the corresponding mechanism.

5.
Front Microbiol ; 13: 1044009, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36312967

RESUMO

In this work, the removal efficiency and mechanism of various constructed wetlands microcosm systems on antibiotic wastewater, as well as the biological community response of microalgae and microorganisms were explored. Overall, the algal-bacteria symbiosis in conjunction with the gravel matrix had the most comprehensive treatment efficiency for antibiotic wastewater. However, pollutants such as high-concentration antibiotics impaired the biological community and functions. In the systems fed with microorganisms, both abundance and diversity of them were significantly reduced comparing with the initial value. According to the correlation analysis revealed that the pollutants removal rate increased with the addition of the relative abundance of some bacterial genera, while decreased with the addition of relative abundance of other bacterial genera. The presence of gravel matrix could lessen the stressful effect of antibiotics and other pollutants on the growth of microalgae and microorganisms, as well as improved treatment efficiency of antibiotic wastewater. Based on the findings of the study, the combination of gravel matrix and algal-bacteria symbiosis can considerably increase the capacity of constructed wetlands to treat antibiotic wastewater and protect biological community, which is an environmentally friendly way.

6.
J Phys Chem Lett ; 13(41): 9607-9617, 2022 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-36206518

RESUMO

Operando reconstruction of solid catalyst into a distinct active state frequently occurs during electrocatalytic processes. The correlation between initial and operando states, if ever existing, is critical for the understanding and precise design of a catalytic system. Inspired by recently established intermediate metallic state of Bi-based catalysts during electrocatalytic carbon dioxide reduction (CO2RR), here we investigate a series of Bi oxide catalysts (Bi, Bi2O3, BiO2) and demonstrate that the operando surface/subsurface oxygen loading, positively correlated to the initial oxygen content, plays a critical role in determining Bi-based CO2RR performance. Higher initial oxygen loading indicates a better electrocatalytic efficiency. Further analysis shows that this conclusion generally applies to all Bi-based electrocatalysts reported up to date. Following this principle, cost-effective BiO2 nanocrystals demonstrated the highest formate Faradaic efficiency (FE) and current density compared to Bi/Bi2O3, further allowing a pair-electrolysis system with 800 mA/cm2 current density and an overall 175% FE for formate production.

7.
Ecotoxicol Environ Saf ; 245: 114123, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-36183427

RESUMO

Cadmium (Cd) is a type of high-risk heavy metal that can damage organs such as the liver, but its mechanism is not yet clear. Ferroptosis is a newly discovered mode of regulatory cell death. We explored whether ferroptosis is involved in Cd-induced liver damage and the underlying mechanism. Our research showed that Cd induced liver damage by inducing ferroptosis, and the use of ferroptosis inhibitors reduced the degree of liver damage. Moreover, the occurrence of ferroptosis was accompanied by the activation of the PERK-eIF2α-ATF4-CHOP signaling pathway, and inhibiting endoplasmic reticulum (ER) stress reduced ferroptosis demonstrating that ferroptosis induced by Cd is dependent on ER stress. In addition, chloroquine, a common autophagy inhibitor, mitigated ferroptosis caused by Cd exposure. Then, the iron chelator deferoxamine reduced Cd-induced lipid peroxidation and cell death, demonstrating that the iron regulation disorder caused by ferritin phagocytosis contributes to the Cd-induced ferroptosis. In conclusion, our results show that Cd-induced liver toxicity is accompanied by ferroptosis, which contributes to Cd inducing oxidative stress to trigger autophagy and ER stress to promote the process of ferroptosis.


Assuntos
Ferroptose , Hepatopatias , Autofagia , Cádmio/metabolismo , Cádmio/toxicidade , Cloroquina , Desferroxamina , Estresse do Retículo Endoplasmático , Ferritinas , Humanos , Ferro/metabolismo , Quelantes de Ferro
8.
Microorganisms ; 10(9)2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36144324

RESUMO

A sudden large-scale bloom event of the haptophyte Phaeocystis globosa that lasted over one month in the winter of 2021 was observed offshore Qingdao, China. This P. globosa bloom event was unusual as it was the first P. globosa bloom recorded in Qingdao offshore. Furthermore, the temperature at which this event occurred was much lower than that of previous P. globosa blooms in China. We hypothesize that the P. globosa strains that drove the development of this bloom offshore Qingdao were genetically unique and have a competitive advantage in the environmental conditions. To test this hypothesis, we analyzed P. globosa genetic diversity and the temporal dynamics of the bloom, using the high-resolution molecular markers pgcp1 and cox1 that we developed recently. The analysis revealed that the genetic compositions of P. globosa offshore Qingdao were rather limited, containing two dominant genotypes and other rare genotypes with low abundance, representing a small portion of the genetic diversities identified in coastal waters in China, and were rather different from the P. globosa genotypes outside of the Jiaozhou Bay before the P. globosa bloom in the winter of 2021. This suggested only certain strains contribute to the development of blooms under certain environmental conditions. The genetic composition may indicate the unusual timing and scale of this P. globosa event.

9.
Phys Chem Chem Phys ; 24(36): 21588-21592, 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36069424

RESUMO

Proteins tune the reactivity of metal sites; less understood is the impact of association with a redox partner. We demonstrate the utility of carbon-deuterium labels for selective analysis of delicate metal sites. Introduced into plastocyanin, they reveal substantial strengthening of the key Cu-Cys89 bond upon association with cytochrome f.


Assuntos
Cobre , Plastocianina , Carbono , Cobre/química , Citocromos f/metabolismo , Deutério , Oxirredução , Plastocianina/química , Plastocianina/metabolismo
10.
Comput Biol Med ; 149: 106064, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36084383

RESUMO

Structural flexibility is an intrinsic characteristics of a protein upon interacting with other molecules, which mainly comes from the movement of a residue's side chain, backbone and even an entire domain. Considering this property can be very helpful in protein binding analysis, such as epitope identification during antibody-antigen interaction. Unfortunately, to our knowledge, no approach is available at studying the dynamicity of protein binding from the computational perspective. We are pioneering a new perspective of exploring protein binding sites with considering the structural flexibility, particularly from the epitopes identification angle in antibody-antigen binding. To this end, we first obtained protein antigen structures with epitopes available, and built residue-level graphs of antigens. These graphs were highly densified subsequently by incorporating the structural flexibility. Later, the edge enriched graphs were clustered into overlapping subgraphs and were classified as epitope or non-epitope by a graph convolutional network. Experiments on epitope identification shown that the proposed flexibility-aware model markedly outperformed existing approaches by lifting the F1-score to 0.656, making a remarkable increment of 16.3% compared to the state-of-the-art. A quick study on generic protein binding site prediction also made a noteworthy improvement with increasing the F1-score by 8%. The superior performance obtained from both the specific and generic protein interaction analysis demonstrate that incorporating flexibility in computational models is helpful to strength the capability of identifying epitopes as well as general protein binding sites. This seminal study can be inspiring and promising to the wide range of protein interaction analysis.


Assuntos
Antígenos , Epitopos de Linfócito B , Antígenos/química , Sítios de Ligação , Epitopos de Linfócito B/química , Ligação Proteica
11.
J Am Chem Soc ; 144(33): 15185-15192, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-35948416

RESUMO

Selective oxidation to synthesize nitriles is critical for feedstock manufacturing in the chemical industry. Current strategies typically involve substitutions of alkyl halides with toxic cyanides or the use of strong oxidation reagents (oxygen or peroxide) under ammoxidation/oxidation conditions, setting considerable challenges in energy efficiency, sustainability, and production safety. Herein, we demonstrate a facile, green, and safe electrocatalytic route for selective oxidation of amines to nitriles under ambient conditions, assisted by the anodic water oxidation on metal-doped α-Ni(OH)2 (a typical oxygen evolution reaction catalyst). By controlling the balance between co-adsorption of the amine molecule and hydroxyls on the catalyst surface, we demonstrate that Mn doping significantly promotes the subsequent chemical oxidation of amines, resulting in Faradaic efficiencies of 96% for nitriles under ≥99% conversion. This anodic oxidation is further coupled with cathodic hydrogen evolution for overall atomic economy and additional green energy production.

12.
Toxicol Appl Pharmacol ; 451: 116190, 2022 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-35917840

RESUMO

Acrylamide (ACR) as a by-product of Maillard reaction is widely present in food. Although ACR is known to exhibit neurotoxicity, most studies about ACR neurotoxicity are currently short-term high-dose providing limited reference value for human exposure. The present study aims to determine the effects of chronic ACR exposure on dopaminergic neurons in rat nigra and the potential mechanism from the perspective of NLRP3 inflammasome-mediated neuroinflammation. The SD rats were maintained on treated drinking water providing dosages of 0, 0.5, or 5 mg/kg/day ACR for 12 months. ACR exposure caused motor dysfunction in rats, which was associated with dopaminergic neuron loss, α-Synuclein (α-Syn) accumulation and decreased brain-derived neurotrophic factor (BDNF) in nigra. ACR activated microglia by increasing Iba-1+, Iba-1+CD68+ positive cells and the percentage of ameboid-shaped ones in rat nigra. ACR markedly upregulated the protein levels of NLRP3 inflammasome constituents NLRP3 and caspase-1 and inflammatory cytokine IL-1ß. ACR chronic exposure increased the risk of Parkinson's disease (PD) like dopaminergic neuron depletion in nigra potentially through NLRP3 inflammasome-mediated neuroinflammtion.


Assuntos
Neurônios Dopaminérgicos , Transtornos Motores , Acrilamida/toxicidade , Animais , Dopamina/metabolismo , Humanos , Inflamassomos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Microglia , Transtornos Motores/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Degeneração Neural/metabolismo , Doenças Neuroinflamatórias , Ratos , Ratos Sprague-Dawley
13.
Front Immunol ; 13: 936707, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958546

RESUMO

Background: Vogt-Koyanagi-Harada (VKH) disease is an autoimmune inflammatory disorder characterized by bilateral granulomatous uveitis. The objective of this study was to identify immune hub genes involved in the pathogenesis and progression of VKH disease. Methods: High throughput sequencing data were downloaded from the Gene Expression Omnibus (GEO) and an immune dataset was downloaded from ImmPort. Immune differentially expressed genes (DEGs) were obtained from their intersection in the GEO and ImmPort datasets. Immune hub genes for VKH disease were selected through differential expression analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Disease Ontology (DO), protein-protein interaction (PPI) network, and clustering analyses. Confidence in the immune hub genes was subsequently validated using box plots and receiver operating characteristic (ROC) curves. Results: A total of 254 DEGs were screened and after the intersection with ImmPort, 20 genes were obtained as immune DEGs. Functional enrichment analysis indicated that the key genes were mainly involved in several types of immune pathways (such as the lymphocyte mediated and leukocyte mediated immune responses, natural killer cell mediated cytotoxicity, and antigen binding) and immunodeficiency diseases. Following PPI network analysis, the top seven genes in cluster 1 were selected as potential immune hub genes in VKH. After evaluating the accuracy of the hub genes, one gene (GNLY) was excluded because its expression level was statistically similar in VKH patients and healthy controls. Finally, six immune hub genes, namely KLRC2, KLRC3 SH2D1B, GZMB, KIR2DL3, and KIR3DL2 were identified as playing important roles in the occurrence and development of VKH disease. Conclusion: Six immune hub genes (KLRC2, KLRC3 SH2D1B, GZMB, KIR2DL3, and KIR3DL2) identified by our bioinformatics analyses may provide new diagnostic and therapeutic targets for VKH disease.


Assuntos
Síndrome Uveomeningoencefálica , Análise por Conglomerados , Biologia Computacional , Ontologia Genética , Humanos , Subfamília C de Receptores Semelhantes a Lectina de Células NK , Mapas de Interação de Proteínas/genética , Síndrome Uveomeningoencefálica/genética
14.
Biomater Sci ; 10(20): 5938-5946, 2022 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-36043429

RESUMO

Retinal progenitor cell (RPC) transplantation has been proposed as a potential strategy for the treatment of retinal degeneration, which is a leading cause of vision loss. However, a major obstacle is the poor proliferation of RPCs. Accumulating evidence suggests that the chiral features of the extracellular microenvironment are closely related to cell proliferation. Inspired by this, L/D-phenylalanine-derived molecules (LP and DP) are employed to construct a biomimetic chiral microenvironment for enhancing RPC proliferation. LP and DP self-assemble into left-handed and right-handed helical fibrous networks, respectively. It is found that DP nanofibrous films show an excellent ability in promoting RPC proliferation via the activation of the Akt and extracellular signal-regulated kinase (ERK) pathways. In addition, both LP and DP nanofibrous films have the advantage of attenuating inflammation, and LP films can maintain the stem potential of RPCs. Thus, the promotion of RPC proliferation using a bioinspired chiral fibrous microenvironment is a promising strategy for RPC-based therapies for retinal degeneration.


Assuntos
MAP Quinases Reguladas por Sinal Extracelular , Degeneração Retiniana , Diferenciação Celular/fisiologia , Proliferação de Células/fisiologia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Humanos , Sistema de Sinalização das MAP Quinases , Fenilalanina/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Degeneração Retiniana/metabolismo , Células-Tronco
15.
Front Immunol ; 13: 871713, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35844539

RESUMO

Autoimmune diseases are a series of diseases involving multiple tissues and organs, characterized by the over production of abnormal multiple antibodies. Although most studies support that the impaired immune balance participates in the development of autoimmune diseases, the specific pathogenesis of it is not fully understood. Intestinal immunity, especially the intestinal mucosal barrier has become a research hotspot, which is considered to be an upstream mechanism leading to the impaired immune balance. As an important defense barrier, the intestinal mucosal barrier regulates and maintains the homeostasis of internal environment. Once the intestinal barrier function is impaired under the effect of multiple factors, it will destroy the immune homeostasis, trigger inflammatory response, and participate in the development of autoimmune diseases in the final. However, the mechanism of the intestinal mucosal barrier how to regulate the homeostasis and inflammation is not clear. Some studies suggest that it maintains the balance of immune homeostasis through the zonulin pathway, intestinal microbiome, and Toll-like receptor signaling pathway. Our review focused on the composition and the function of the intestinal mucosal barrier to describe the research progress of it in regulating the immune homeostasis and inflammation, and also pointed that the intestinal mucosal barrier was the potential targets in the treatment of autoimmune diseases.


Assuntos
Doenças Autoimunes , Microbioma Gastrointestinal , Doenças Autoimunes/metabolismo , Humanos , Inflamação/metabolismo , Mucosa Intestinal , Intestinos
16.
Polymers (Basel) ; 14(13)2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-35808663

RESUMO

The preforming quality of carbon fiber plain-woven thermoset prepreg (CFPWTP) is critical to the performance of composite aerospace parts. The deformation ability of the CFPWTP material during preforming is affected by both the fabric woven structure and the resin viscosity, which is different from the dry textile material. Incorrect temperature parameters can enlarge the resin's viscosity, and high viscosity can inhibit fiber deformation and cause defects. This study proposes an equivalent continuum mechanics model considering its temperature-force behavior. Picture frame tests and axial tensile tests at 15 °C, 30 °C, and 45 °C are conducted to obtain the temperature-stress-strain constitutional equations. By Taylor's expansion formula and surface fitting method, the constitutive modulus of the material is obtained. Consequently, a saddle-shaped forming simulation is carried out, which is later validated by experiments. Results show that the accuracy of the predicted model is high, with 0.9% of width error and 5.1% of length error separately. Besides, the predicted wrinkles are consistent with the test in fold position and in deformation trend under different temperatures.

17.
Front Mol Biosci ; 9: 885806, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35755807

RESUMO

Glioblastoma (GBM) is the most common malignant primary brain tumor in adults, which is fast growing and tends to invade surrounding normal brain tissues. Uncovering the molecular and cellular mechanisms of GBM high invasion potential is of great importance for the treatment and prognostic prediction. However, the commonly used two-dimensional (2D) cell culture and analysis system suffers from lack of the heterogeneity and in vivo property of brain tissues. Here, we established a three-dimensional (3D) cell culture-based analysis system that could better recapitulate the heterogeneity of GBM and mimic the in vivo conditions in the brain. The GBM cell lines, DBTRG and U251, were cultured by hanging drop culture into the GBM multicellular spheroids, which were embedded in the optimized 3D brain-stiffness-mimicking matrix gel (0.5 mg/ml Collagen Ⅰ + 3 mg/ml Matrigel+ 3.3 mg/ml Hyaluronic Acid (HA)). The biochemical composition of the optimized matrix gel is similar to that of the brain microenvironment, and the elastic modulus is close to that of the brain tissue. The dynamics of the GBM spheroids was examined using high-content imaging for 60 h, and four metrics including invasion distance, invasion area, single-cell invasion velocity, and directionality were employed to quantify the invasion capacity. The result showed that DBTRG cells possess higher invasion capacity than U251 cells, which was consistent with the results of the classic transwell test. Transcriptome analysis of both cell lines was performed to explore the underlying molecular mechanisms. Our novel brain-stiffness-mimicking matrix gel enables comprehensive invasion analysis of the 3D cultured GBM cells and provides a model basis for in-depth exploration of the mechanisms regulating GBM invasion including the interaction between GBM cells and brain stroma.

18.
J Hepatocell Carcinoma ; 9: 389-403, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35592243

RESUMO

Purpose: The aim of this study was to identify and validate novel biomarkers for distinguishing among hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), liver fibrosis/liver cirrhosis (LF/LC) and chronic hepatitis B (CHB). Patients and Methods: Transcriptomic sequencing was conducted on the liver tissues of 5 patients with HCC, 5 patients with LF/LC, 5 patients with CHB, and 4 healthy controls. The expression levels of selected mRNAs and proteins were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical (IHC) staining, and were verified in validation set (n=200) and testing set (n=400) via enzyme-linked immunosorbent assay (ELISA). Results: A total of 9 hub mRNAs were identified by short time-series expression miner and weighted gene co-expression network analysis. Of note, the results of qRT-PCR and IHC staining demonstrated that SHC adaptor protein 1 (SHC1), SLAM family member 8 (SLAMF8), and interleukin-32 (IL-32) exhibited gradually increasing trends in the four groups. Subsequent ELISA tests on the validation cohort indicated that the plasma levels of SHC1, SLAMF8 and IL-32 also gradually increased. Furthermore, a diagnostic model APFSSI (age, PLT, ferritin, SHC1, SLAMF8 and IL-32) was established to distinguish among CHB, LF/LC and HCC. The performance of APFSSI model for discriminating CHB from healthy subjects (AUC=0.966) was much greater compared to SHC1 (AUC=0.900), SLAMF8 (AUC=0.744) and IL-32 (AUC=0.821). When distinguishing LF/LC from CHB, APFSSI was the most outstanding diagnostic parameter (AUC=0.924), which was superior to SHC1, SLAMF8 and IL-32 (AUC=0.812, 0.684 and 0.741, respectively). Likewise, APFSSI model with the greatest AUC value displayed an excellent performance for differentiating between HCC and LF/LC than other variables (SHC1, SLAMF8 and IL-32) via ROC analysis. Finally, the results in the test set were consistent with those in the validation set. Conclusion: SHC1, SLAMF8 and IL-32 can differentiate among patients with HCC, LF/LC, CHB and healthy controls. More importantly, the APFSSI model greatly improves the diagnostic accuracy of HBV-associated liver diseases.

19.
Polymers (Basel) ; 14(9)2022 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-35567024

RESUMO

Carbon fiber plain-woven prepreg is one of the basic materials in the field of composite material design and manufacturing, in which defect identification is an important and easily neglected part of testing. Here, a novel high recognition rate inspection method for carbon fiber plain-woven prepregs is proposed for inspecting bubble and wrinkle defects based on image texture feature compression. The proposed method attempts to divide the image into non-overlapping block lattices as texture primitives and compress them into a binary feature matrix. Texture features are extracted using a gray level co-occurrence matrix. The defect types are further defined according to texture features by k-means clustering. The performance is evaluated in some existing computer vision and machine learning methods based on fiber recognition. By comparing the result, an overall recognition rate of 0.944 is achieved, which is competitive with the state-of-the-arts.

20.
Neurol Sci ; 43(9): 5431-5439, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35579823

RESUMO

BACKGROUND/OBJECTIVE: Cardiac diseases are frequently accompanied by elevated levels of biomarkers, among which, troponin is commonly investigated. The levels of plasma cardiac troponin I (cTnI), which has been shown to predict short-term mortality, are elevated in patients with acute cerebral infarction (ACI). However, few studies have assessed the association between cTnI concentration and long-term mortality in patients with ACI following thrombolysis. METHODS: Patients with ACI admitted between January 1, 2014, and December 31, 2016, were registered. Data on demographics and outcomes with elevated cTnI levels were also collected. RESULTS: A total of 145 patients with ACI were recruited; 97 (66%), 30 (20%), and 18 (12%) patients had cTnI concentrations < 0.030 (group 1), 0.030-0.10 (group 2), and > 0.10 µg/L (group 3), respectively. cTnI elevation was associated with older age, atrial fibrillation, congestive heart failure, renal insufficiency, coronary artery disease, stroke severity (National Institutes of Health Stroke Scale score), and prior smoking history at admission. After adjusting for comorbidities and severity at 3 months after ACI, cTnI elevation on admission was significantly associated with ascending 5-year mortality (hazard ratio, 1.80; 95% confidence interval, 1.22-2.65). CONCLUSIONS: Even after adjusting for several possible confounders, cTnI elevation in patients with ACI treated with rt-PA was associated with a 1.80-fold increased risk of 5-year mortality.


Assuntos
Isquemia Encefálica , Acidente Vascular Cerebral , Doença Aguda , Biomarcadores , Infarto Cerebral , Humanos , Prognóstico , Terapia Trombolítica/efeitos adversos , Troponina I
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