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1.
J Nanosci Nanotechnol ; 20(2): 828-839, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383078

RESUMO

At present, high-performance cement-based composites are widely used, and they are prone to early cracking due to their high autogenous shrinkage stress. In this research, the uniformly dispersed GNPs were added into high-performance cementitious materials. The autogenous shrinkage of high-performance cementitious matrix materials with different incorporation of GNPs was also researched with water to cement ratio of 0.25, 0.30 and 0.35. According to hydration heat, hydration products, microstructure and porosity of GNPs cementitious matrix materials, the microcosmic mechanism for autogenous shrinkage was also investigated. It was testified that moderate addition of GNPs decreased the autogenous shrinkage of cement-based composites. Moreover, the autogenous shrinkage value was minimal after treatment with 0.10 wt% GNPs cement paste sample for 7 days, at the water to cement ratio of 0.35, and the depressed percentage of autogenous shrinkage reached 81.60% compared with the blank sample.

2.
Inorg Chem ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31503467

RESUMO

Thiospinel AgIn5S8 as a visible-light-active semiconductor has been frequently used as a photoabsorber in solar cells, optoelectronics devices, and photoelectrochemical cells. Similar to temperature, pressure is an efficient external stimulus for both crystalline structural and electronic modulations to improve properties. Herein, we present the pressure tuning effect on AgIn5S8 up to 40 GPa. A pressure-driven phase transition from the ambient cubic spinel structure to an orthorhombic structure is observed around 10 GPa as evidenced from the in situ high pressure synchrotron X-ray diffraction results. The high pressure phase of AgIn5S8 adopts the defective LiVO2-type structure with all the Ag+/In3+ cations sitting in the octahedrally coordinated environments. Both the electric transport and photocurrent measurements show dramatic changes along with the phase transition around 10 GPa, and the high pressure phase of AgIn5S8 exhibits greatly improved conductivity but decreased responses to visible light illumination. Surprisingly, the in situ UV-vis measurements reveal the onset pressure point of bandgap evolution around 7.5 GPa, far below the structural phase transition pressure around 10 GPa, which indicates the early initiated local structural change in the pressure range 7.5-10 GPa. An in situ Raman technique is used to confirm the coordination environment changes of AgIn5S8 under compression, the results of which reveal the coexistence of both the ambient and the high pressure structure features of AgIn5S8 in the pressure range 7.5-10 GPa. This work provides a demonstration on how external pressure affects the crystal structure, electronic structure, and optical properties of chalcogenide semiconductors and sheds light on the structure design of better optoelectrical materials under ambient conditions.

3.
Traffic Inj Prev ; : 1-6, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31442087

RESUMO

Objective: Taxis provide an important mode of public transport in China, but there has been very little in-depth research on the crash involvement propensity of taxi drivers. Thus, this study was conducted to develop a quantitative model for predicting the crash rate of taxi drivers. Methods: A total of 2,391 taxi drivers from 29 companies in 4 Chinese cities completed a structured and anonymous face-to-face questionnaire reporting their demographic information, workload conditions, aberrant driving behaviors, and crash history within the 2 years prior to the survey. Using the self-reported variables, a negative binomial model was implemented to predict taxi drivers' property damage only (PDO) and personal injury (PI) crash rates and identify the factors contributing to this risk. Results: Descriptive analysis of the survey data revealed that the workload of taxi drivers in China is relatively heavy. Seven significant predictors of PDO and PI crash rates were identified, including crossing red lights, dangerous overtaking, honking at a slow driver, failure to use an indicator lamp, driving while fatigued, stopping in forbidden areas to pick up or drop off a passenger, and driving with one hand. Taxi drivers' average off-duty days per week, aggressive driving behaviors, and preventing another driver from merging had significant effects only on PDO crash rate, and sleep problems were found to be significantly correlated with PI crash rate. Conclusions: To improve the safety of taxi drivers, considerable measures should be strictly implemented, ranging from periodic driver training and safety education to workload reduction, with the cooperation of government agencies and taxi companies. The findings of this study contribute to the design of potentially useful policy initiatives as well as targeted safety promotion programs.

4.
Eur J Surg Oncol ; 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31445769

RESUMO

INTRODUCTION: Synovial sarcoma (SS) is a tumor of unknown origin and is extremely rare in the central nervous system. Most studies on intracranial SS included only one or two cases. To better understand the disease, we review a series of primary intracranial SS. METHOD AND MATERIALS: 16 primary intracranial SS in Tiantan Hospital during 2008-2017 were included. The clinical characteristics, including radiological and histological examination, operative records, and prognoses were reviewed. RESULT: The case series included nine male and seven female patients with an average age of 23.8 years. Radiological results showed that the supratentorial region (81.25%) was the most common site of the brain involved. All patients were misdiagnosed as non-SS tumors. Gross total resection (GTR) was achieved in 12 cases (75.0%), and subtotal resection (STR) was achieved in 4 cases. All cases showed the characteristic SYT-SSX fusion gene, as detected by RT-PCR. The mean progression-free survival time (PFS) was 10.0 months and the mean overall survival time (OS) was 15.5 months. Multivariate analysis revealed that GTR and postoperative adjuvant radiotherapy were independent factors for PFS (HR = 6.143, 95% CI = 1.491-25.312; P = 0.012, HR = 6.143, 95% CI = 1.491-25.312; P = 0.012 respectively) and OS (HR = 9.000, 95% CI = 1.627-49.773; P = 0.012, HR = 0.017, 95% CI = 0.001-0.213; P = 0.002 respectively). CONCLUSION: Intracranial SS were more frequently observed in the supratentorial region and in young patients without sex predilection. We recommend adjuvant radiation regardless of the extent of resection. More patients and longer follow-up periods were needed to further elucidate the biological features of intracranial SS.

5.
ACS Appl Mater Interfaces ; 11(31): 27813-27822, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31291080

RESUMO

The Mn-based mixed polyanion is expected to be a promising cathode material for sodium-ion batteries applied to large-scale smart grid energy storage systems due to its stable three-dimensional crystal structure, low cost, and high energy density. Herein, a novel carbon nanotube (CNT)-modified mixed-polyanion material (Na4Mn2Co(PO4)2P2O7) with a high voltage of 3.86 V is synthesized by a facile spray-drying method. The well-designed Na4Mn2Co(PO4)2P2O7/C-CNTs microsphere has excellent electronic and ionic conductivity by virtue of the carbon nanotube conductive skeleton. The as-prepared Na4Mn2Co(PO4)2P2O7/C-CNTs composite exhibits a reversible initial discharge capacity of 96.1 mA h g-1 and an energy density of 371 Wh kg-1 at 0.1 C. Furthermore, Na4Mn2Co(PO4)2P2O7/C-CNTs and hard carbon are assembled into a full battery, which delivers an initial discharge capacity of 88.8 mA h g-1, a working voltage of 3.85 V, and a promising energy density of 249.9 Wh kg-1 at 0.1 C. Therefore, the outstanding performance makes the Na4Mn2Co(PO4)2P2O7/C-CNTs material a potential candidate for large-scale applications of sodium-ion batteries.

6.
Artif Cells Nanomed Biotechnol ; 47(1): 3067-3071, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31349749

RESUMO

Long non coding RNAs (lncRNAs) are important in the occurrence and development of various cancers. They have been considered to participate in many processes of diseases. In this study, we aimed at investigating expression level and clinical significance of lncRNA X91348 in hepatocellular carcinoma (HCC). The expression of X91348 in tissue and serum samples from patients with HCC and from healthy people was detected through quantitative real-time polymerase chain reaction (qRT-PCR). X91348 expression was decreased in patients with HCC compared with healthy controls no matter in tissue or serum samples. The relationship between X91348 expression and clinicopathologic characteristics was analysed. The result demonstrated that tumour size, HBsAg and Child-Pugh were vital influencing factors for X91348 expression, which revealed X91348 may be involved in the progress of HCC. Kaplan-Meier analysis was used to evaluate overall survival of patients with different expressions of X91348. Finally, prognostic value of X91348 in HCC was assessed via cox regression analysis. X91348 was proved to be closely related to the prognosis of HCC. Taken together, the down-regulation of X91348 could be an independent diagnostic and prognostic indicator for HCC.

7.
J Cardiothorac Surg ; 14(1): 143, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31340823

RESUMO

BACKGROUNDS: The pulmonary ciliated muconodular papillary tumor (CMPT) is a very rare tumor with only several case reports in published literatures, and its clinicopathological features, standard treatment methods and prognosis has not been well defined. METHODS: Two cases of CMPT diagnosed and treated in our hospital and 39 cases reported in the published literature were analyzed retrospectively. RESULTS: The cohort of 41 CMPT patients comprised of 20 males and 21 females, aged 9-84 years. The diameter of the primary tumor was 0.3-4.5 cm. Most of these lesions were subsolid nodules, as observed on computed tomography and easily misdiagnosed as early lung adenocarcinoma. Tumors of 26 patients were stained by immunohistochemistry method, which revealed that CK7, CEA, and TTF-1 were positive and CK20 was negative in most patients. The results of gene alternation demonstrated mutations in EGFR, KRAS, and BRAF and ALK rearrangements in CMPT. All the patients underwent surgical treatment and did not receive postoperative adjuvant therapy. The follow-up duration was 0-120 months, and no case of tumor recurrence was found until the final follow-up. CONCLUSIONS: The incidence of CMPT was low and rate of image misdiagnosis high. Immunohistochemistry is helpful for accurate diagnosis of CMPT. Sub-lobectomy may be proper and adjuvant treatment should be avoided since the disease is now prone to benign lesions. Furthermore, since the biological behavior of this tumor is not yet fully elucidated, additional case data are essential for accurate conclusions.


Assuntos
Adenocarcinoma de Pulmão/patologia , Adenocarcinoma Mucinoso/patologia , Neoplasias Pulmonares/patologia , Adenocarcinoma de Pulmão/diagnóstico por imagem , Adenocarcinoma de Pulmão/genética , Adenocarcinoma de Pulmão/cirurgia , Adenocarcinoma Mucinoso/diagnóstico por imagem , Adenocarcinoma Mucinoso/genética , Adenocarcinoma Mucinoso/cirurgia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Cílios/patologia , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Mutação , Prognóstico , Estudos Retrospectivos , Tomografia Computadorizada por Raios X , Adulto Jovem
8.
Life Sci ; 232: 116594, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31233761

RESUMO

AIMS: Sleep deficiency has been reported to be associated with some oral health problems. Oral ulcers are very common lesions of the oral mucosa, which severely impact patients' quality of life. However, the association between sleep deficiency and the oral ulcer remains unknown. The present study aims to explore the effects of sleep deficiency on oral ulcers. MAIN METHODS: Rats were divided into normal control group (n = 30) and oral ulcer group (OU group, n = 50). Model rats with phenol-induced oral ulcers were deprived of sleep for 72 h by using the modified multiple platform technique. KEY FINDINGS: Sleep deprivation worsened oral ulcers and delayed healing process in rats. In addition, sleep deprivation increased the γ-aminobutyric acid (GABA, P < 0.01) and 5-hydroxytryptamine (5-HT, P < 0.05) levels in serum and brain, the corticotrophin (ACTH, P < 0.05), corticosterone (CORT, P < 0.01), immunoglobulin (Ig)M (P < 0.01), tumor necrosis factor-alpha (TNF-α) (P < 0.01), interleukin (IL)-1ß (P < 0.01), IL-6 (P < 0.01), IL-8 (P < 0.01), monocyte chemoattractant protein-1 (MCP-1) (P < 0.01), and 8-hydroxy-deoxyguanosine (8-OHdG, P < 0.01) levels in serum. Sleep deprivation also up-regulated malonaldehyde (MDA) (P < 0.05), TNF-α (P < 0.05), and IL-1ß (P < 0.01) levels in oral mucosa tissue and delayed superoxide dismutase (SOD, P < 0.05) activity recovery. SIGNIFICANCE: These data suggest that sleep deprivation impaired the oral ulcer healing in rat oral mucosa, and the mechanisms of this effect are probably related to neuro-immuno-endocrine system and oxidative stress.

9.
ChemSusChem ; 12(16): 3817-3826, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31237111

RESUMO

Olivine-structured LiMnPO4 (LMP) is an efficient Li+ host owing to its high theoretical energy density and thermal stability. However, its poor ionic and electronic conductivity severely hinder its practical application. Herein, one-dimensional (1D) LMP@C nanofibers with in situ created 3D mesoporous architecture are reported and the charge-storage behavior is addressed. Ultrafine LMP nanoparticles are homogeneously confined in the nanofibers with interconnected and exposed mesoporous intersections, facilitating the electronic/ionic transportation and retarding the pulverization/fracture of electrodes. Remarkably, the hierarchical construction promotes a certain degree of pseudocapacitive contribution. The diffusion-controlled battery-type and surface-controlled capacitive faradaic redox processes act synergistically, giving new insights into Li-ion storage cathode materials to reach the common goal of high energy density and power density simultaneously. The current separation technique suggests surface-dominated pseudocapacitance as the major Li+ storage mechanism at high rates, which is regarded as an efficient way to improve the rate performance. Hence, the as-prepared LMP@C nanofibers could deliver a high reversible capacity of 149.8 mAh g-1 with 92 % charge retention over 300 cycles at 0.2 C (1 C=171 mA g-1 ). Even at a high rate of 5 C, a capacity of 63.1 mAh g-1 is retained after 2000 cycles with an exceptional cyclic stability.

10.
ACS Appl Mater Interfaces ; 11(23): 20796-20803, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-31090395

RESUMO

Aqueous rechargeable zinc batteries (ARZBs) are desirable for energy storage devices owing to their low cost and abundance of the Zn anode, but their further development is limited by a dearth of ideal cathode materials that can simultaneously possess high capacity and stability. Herein, we employ a layered structure of ammonium vanadium bronze (NH4)0.5V2O5 as the cathode material for ARZBs. The large interlayer distance supported by the NH4+ insertion not only facilitates the Zn2+-ion intercalation/deintercalation but also improves the electrochemical stability in ARZBs. As a result, the layered structural (NH4)0.5V2O5 cathode delivers a high capacity up to 418.4 mA h g-1 at a current density of 0.1 A g-1. A reversible capacity of 248.8 mA h g-1 is still retained after 2000 cycles and a capacity retention of 91.4% was maintained at 5 A g-1. Furthermore, in comparison with previously reported Zn-ion batteries, the Zn/(NH4)0.5V2O5 battery achieves a prominent high energy density of 418.4 W h kg-1 while delivering a high power density of 100 W kg-1. The results would enlighten and push the ammonium vanadium compounds to a brand new stage for the application of aqueous batteries.

11.
Sci Rep ; 9(1): 6607, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31036927

RESUMO

The study was designed to assess a practical mode of postdilution continuous venovenous hemofiltration (CVVH) with regional citrate anticoagulation (RCA) using a calcium-containing replacement solution, and to compare it with a CVVH mode with no anticoagulation (NA). Both methods were employed in our center for acute kidney injury (AKI) patients at high risk of bleeding. Fifty-six patients were equally allocated into the RCA-CVVH group and the NA-CVVH group. The study displayed no significant differences between groups involving baseline characteristics, severity level, blood gas analysis, hepatic/renal/coagulative functions, electrolytes, hemoglobin concentration, and platelet counts before or after continuous renal replacement therapy (CRRT). Compared to the NA-CVVH group, the RCA-CVVH group had a lower level of transfused packed red blood cells and platelet as well as a longer filter lifespan. The result showed no substantial differences between groups in terms of the mean supporting time and cost involving CRRT per person, the length of ICU and hospital stays, and the ICU survival. Homeostasis was basically preserved at a target range during the RCA post-CVVH procedure. Serious complications did not arise during the RCA process. RCA postdilutional CVVH is a safe and effective mode for application in AKI patients with a high risk of bleeding, and it can extend the filter lifespan and decrease blood loss, compared with the NA mode for CRRT. Further studies are needed to evaluate this mode for CRRT. (Retrospective Registration number ChiCTR1800016462, Registration date 2/6/2018).

13.
Gene ; 707: 136-142, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31054361

RESUMO

Neural stem/progenitor cells (NSPCs) can enhance regeneration after spinal cord injury (SCI), but survival of transplanted cells remains poor. Understanding how NSPCs respond to the chemical mediators of secondary injury thus is essential for treating SCI. Thymosin ß4 (Tß4) has physiological functions that are highly relevant to SCI. We exposed NSPCs to oxidative stress and found reduced expression of Tß4 in H2O2-injured NSPCs. Using an MTT assay, we found that Tß4 dose dependently increased viability of the injured NSPCs. Tß4 also reversed the decreases of intracellular Ca2+ concentration and increases of lactate dehydrogenase in NSPCs induced by H2O2 treatment. H2O2 exposure increased NSPC apoptosis, which Tß4 decreased. In H2O2-induced NSPCs, ROS production and pro-inflammatory cytokines increased, and again, Tß4 reversed these effects. We investigated the toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (MyD88) signaling pathway as an underlying mechanism in Tß4's protective effect on H2O2-exposed NSPCs. Our results showed that Tß4 reduced expression of TLR4 and MyD88. Moreover, H2O2-exposed NSPCs that were treated with the TLR4/MyD88 pathway inhibitor showed a reversal of all the effects caused by H2O2, similar to Tß4's effects. In conclusion, our study determined that Tß4 attenuated H2O2-induced oxidative stress injury in NSPCs via the TLR4/MyD88 pathway.


Assuntos
Células-Tronco Neurais/citologia , Transdução de Sinais , Traumatismos da Medula Espinal/metabolismo , Timosina/metabolismo , Animais , Sobrevivência Celular , Células Cultivadas , Modelos Animais de Doenças , Regulação para Baixo , Peróxido de Hidrogênio/efeitos adversos , Masculino , Fator 88 de Diferenciação Mieloide/metabolismo , Células-Tronco Neurais/efeitos dos fármacos , Células-Tronco Neurais/metabolismo , Estresse Oxidativo , Ratos , Ratos Sprague-Dawley , Receptor 4 Toll-Like/metabolismo
14.
Angew Chem Int Ed Engl ; 58(28): 9517-9521, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31070857

RESUMO

Exposure of anisotropic crystal facets allows the directional transfer of photoexcited electrons (e- ) and holes (h+ ), for spatial charge separation. High-index facets with a high density of low-coordinated atoms always serve as reactive catalytic sites. However, preparation of multi-facets or high-index facets is highly challenging for layered bismuth-based photocatalysts. Herein, we report the preparation of unprecedented eighteen-faceted BiOCl with {001} top facets and {102} and {112} oblique facets via a hydrothermal process. Compared to the conventional BiOCl square plates with {001} top facets and {110} lateral facets, the eighteen-faceted BiOCl has highly enhanced photocatalytic activity for H2 evolution and hydroxyl radicals (. OH) production. Theoretical calculations and photodeposition results disclose that the of eighteen-faceted BiOCl has a well-matched {001}/{102}/{112} ternary facet junction, which provides a cascade path for more efficient charge flow than the binary facet junction in BiOCl square plates.

15.
Int J Biol Macromol ; 133: 172-183, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30951779

RESUMO

The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways. An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1. The production optimization, purification and characterization of the purified EPS from A. tenuissima F1 and the in-vitro antioxidant potentials was investigated. Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4·7H2O, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 °C, inoculum size of 3%, 180 r/min for 5 d. The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 °C by thermogravimetric analysis (TG/DSC). Molecular weight of the purified EPS was 3.246 × 104 g/mol, and EPS was composed of d-galacturonic acid, rhamnose, d-mannose, glucose, and d-galactose in ratio of 0.45:3.02:3.25:1.0:0.95. The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant.

16.
J Cell Physiol ; 234(11): 19740-19749, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30941768

RESUMO

Long noncoding RNA paternally expressed gene 10 (lncRNA PEG10) has been certified to regulate cell proliferation, metastasis, and apoptosis in diversified cancer cells. Nevertheless, the functions of PEG10 in bladder cancer cells remain uninvestigated. We tried to probe the impacts of PEG10 on proliferation, migration, and invasion of bladder cancer cells. PEG10 expression in SV-HUC-1, T24, RT4, and 253J-BV cells was estimated by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). After pc-PEG10 or sh-PEG10 transfection, the impacts of PEG10 on T24 and 253J-BV cell viability, migration, invasion, and the relevant proteins were disclosed via employing CCK-8, Transwell, and western blot. The relevancy between miR-29b and PEG10 was probed, and the influences of overexpressed miR-29b in above-mentioned cell biological processes were reassessed. Wnt/ß-catenin and JNK pathways were ultimately analyzed to unmake the underling mechanism. We found that overexpressed PEG10 facilitated cell viability and upregulated CyclinD1, CDK4, and CDK6 in T24 and 253J-BV cells. Cell migration and invasion were also elevated by overexpressed PEG10 through the enhancement of MMP-2, MMP-9, and Vimentin. In addition, repressed miR-29b was observed in PEG10-overexpressed T24 and 253J-BV cells. Moreover, overexpressed miR-29b overtly overturned the carcinogenic impacts of PEG10 on T24 cells. The activations of Wnt/ß-catenin and JNK pathways were enhanced by overexpressed PEG10 via mediating miR-29b. SP600125 (JNK inhibitor) disposition reversed the acceerative impacts of PEG10 on cell proliferation, migration, and invasion in T24 cells. In conclusion, the investigations testified that PEG10 gave play to the carcinogenic impacts on bladder cancer cells via mediating miR-29b-Wnt/ß-catenin-JNK axis.

17.
Acta Pharmacol Sin ; 40(9): 1228-1236, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31028291

RESUMO

Bufalin, the major active component of the traditional Chinese medicine ChanSu obtained from the skin and parotid venom glands of toads, has long been known as an anticancer agent. Recent studies show that microRNAs (miRs) are involved in the anticancer activities of bufalin, while long non-coding RNAs (lncRNAs) are known to interact with miRNAs to regulate various biological functions. In this paper, we investigated the possible network related to the antimetastatic effect of bufalin in prostate cancer (PCa) cells. We demonstrated that bufalin (0.05-10 µM) dose-dependently suppressed the proliferation of prostate cancer DU145 and PC3 cells with IC50 values of 0.89 and 1.28 µM, respectively. Furthermore, bufalin treatment significantly suppressed the cell migration and invasion. To explore the role of lncRNAs in the antimetastatic activity of bufalin, we used an lncRNA microarray and found that HOX transcript antisense RNA (HOTAIR) was the most markedly downregulated lncRNA in bufalin-treated PCa cells. Overexpression of HOTAIR counteracted the suppressing effects of bufalin on DU145 and PC3 cells. We then predicted and verified that HOTAIR upregulated FGFR1 expression by sponging miR-520b in PCa cells. In 40 patients with PCa bone metastasis, we used in situ hybridization or immunohistochemical assay to assess the HOTAIR and FGFR1 expression, which revealed that both HOTAIR and FGFR1 expression were significantly higher in bone metastasis tissues than in the primary PCa tissues. In addition, the level of serum HOTAIR was positively associated with the levels of serum bone metabolic markers (CTx, OST, B-ALP and PINP) and may serve as a reasonable biomarker for PCa bone metastasis. Taken together, this is the first study revealing that HOTAIR promotes PCa bone metastasis, and bufalin may be a promising candidate for the treatment of this disease.

18.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 36(2): 245-253, 2019 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-31016941

RESUMO

Vena cava filter is a filter device designed to prevent pulmonary embolism caused by thrombus detached from lower limbs and pelvis. A new retrievable vena cava filter was designed in this study. To evaluate hemodynamic performance and thrombus capture efficiency after transplanting vena cava filter, numerical simulation of computational fluid dynamics was used to simulate hemodynamics and compare it with the commercialized Denali and Aegisy filters, and in vitro experimental test was performed to compare the thrombus capture effect. In this paper, the two-phase flow model of computational fluid dynamics software was used to analyze the outlet blood flow velocity, inlet-outlet pressure difference, wall shear stress on the wall of the filter, the area ratio of the high and low wall shear stress area and thrombus capture efficiency when the thrombus diameter was 5 mm, 10 mm, 15 mm and thrombus content was 10%, 20%, 30%, respectively. Meanwhile, the thrombus capture effects of the above three filters were also compared and evaluated by in vitro experimental data. The results showed that the Denali filter has minimal interference to blood flow after implantation, but has the worst capture effect on 5 mm small diameter thrombus; the Aegisy filter has the best effect on the trapping of thrombus with different diameters and concentrations, but the low wall shear stress area ratio is the largest; the new filter designed in this study has a good filtering and capture efficiency on small-diameter thrombus, and the area ratio of low wall shear stress which is prone to thrombosis is small. The low wall shear stress area of the Denali and Aegisy filters is relatively large, and the risk of thrombosis is high. Based on the above results, it is expected that the new vena cava filter designed in this paper can provide a reference for the design and clinical selection of new filters.


Assuntos
Hemodinâmica , Trombose/terapia , Filtros de Veia Cava , Velocidade do Fluxo Sanguíneo , Humanos , Software , Veia Cava Inferior
19.
Int J Oncol ; 54(6): 2019-2029, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30942439

RESUMO

Osteosarcoma (OS) is the most commonly diagnosed bone tumor in young people with poor prognosis. At present, the mechanisms underlying tumorigenesis in OS are not well understood. The methionine adnosyltransferase 2B (MAT2B) gene encodes the regulatory subunit of methionine adenosyltransferase (MAT). Recent studies demonstrated that it is highly expressed in a number of human malignancies; however, is undefined in OS. In the present study, MAT2B expression was investigated in tumor samples and cell lines. In vivo and in vitro, lentivirus­mediated small hairpin RNA was constructed to target the MAT2B gene and examine the role of MAT2B in OS proliferation. Microarray analysis was performed to examine the possible downstream molecular target of MAT2B in OS. MAT2B was markedly increased in OS specimens compared with the normal bone tissues, and it was additionally abundantly expressed in OS cell lines. Inhibition of MAT2B expression caused a marked decrease in proliferation and significant increase in apoptosis. In vivo, MAT2B silencing significantly inhibited OS cell growth. Microarray analysis suggested that epidermal growth factor receptor (EGFR) and proliferating cell nuclear antigen (PCNA) may function as downstream targets of MAT2B in OS, as confirmed by reverse transcription­quantitative polymerase chain reaction assays and western blotting. Collectively, these results suggested that MAT2B serves a critical role in the proliferation of OS by regulating EGFR and PCNA and that it may be a potential therapeutic target and prognostic factor of OS.


Assuntos
Neoplasias Ósseas/patologia , Metionina Adenosiltransferase/metabolismo , Osteossarcoma/patologia , Antígeno Nuclear de Célula em Proliferação/metabolismo , Animais , Apoptose/genética , Osso e Ossos/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Receptores ErbB/metabolismo , Feminino , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Metionina Adenosiltransferase/genética , Camundongos , Camundongos Nus , Análise de Sequência com Séries de Oligonucleotídeos , Osteossarcoma/genética , RNA Interferente Pequeno/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
20.
Ann Thorac Surg ; 108(3): 873-882, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31026435

RESUMO

BACKGROUND: Only a small series of studies has investigated primary tracheobronchial adenoid cystic carcinoma (TACC), and its prognosis and prognostic factors have not been well defined. METHODS: Patients with TACC diagnosed histologically between January 1967 and December 2017 at our institution were retrospectively analyzed. RESULTS: A total of 191 consecutive patients were included in our study. One hundred sixty-three patients underwent surgery, while the remaining 28 patients received nonoperative therapy. The 5-year, 10-year, and 20-year survival rates of the surgical group were 85.00%, 63.40%, and 47.00%, while the 5-year and 10-year survival rates of the nonoperative group were 63.70% and 46.40%, respectively. The multivariate analysis showed that the complaint duration (<7 months or ≥7 months) and treatment methods (R0 resection, R1 resection with radiotherapy, R1 resection without radiotherapy) were independent prognostic factors for disease-free survival of the R0/1 (resection with no residual tumor or microscopic residual tumor) resected patients, while the tumor size (≤3 cm or >3 cm) and treatment methods were independent prognostic factors for the overall survival. Sixty-four (45.1%) patients in the R0/1 group experienced recurrence/progression, and compared with chemotherapy or supportive treatment, local treatment significantly improved the prognosis of these patients (P < .050). CONCLUSIONS: The complaint duration and tumor size are independent prognostic factors for disease-free survival and overall survival in TACC, respectively. Complete resection of the primary tumor, postoperative adjuvant radiotherapy for patients with positive margins, and local treatment after postoperative recurrence or progression may contribute to better survival.

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