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1.
Artigo em Inglês | MEDLINE | ID: mdl-31897729

RESUMO

BACKGROUND: In light of the controversies regarding the surgical treatment of adult Chiari malformation type I (CM-I) with syringomyelia, a retrospective study was conducted to evaluate the safety and efficacy of tonsillectomy followed by modified reconstruction of the cisterna magna with or without craniectomy. METHODS: Between 2008 and 2017, 78 adult CM-I patients (36 males and 42 females, mean age 40.6 years old) with syringomyelia were treated with posterior fossa decompression (PFD) with tonsillectomy and modified reconstruction of the cisterna magna. Patients were divided into two study groups: group A (n = 40) underwent cranioplasty with replacement of the bone flap; group B (n = 38) underwent suboccipital craniectomy. Neurological outcomes were evaluated by traditional physician assessment (improved, unchanged, and worsened) and the Chicago Chiari Outcome Scale (CCOS). Syringomyelia outcomes were assessed radiologically. RESULTS: The procedure was successfully performed in all patients, and restoration of normal cerebrospinal fluid (CSF) flow was confirmed by intraoperative ultrasonography. The median postoperative follow-up was 20.3 months (range 18-60 months). Clinical improvement was evident in 66 (84.6%) patients, with no significant differences between the two groups (85.0% vs. 84.2%, P = 0.897). According to the CCOS, 36 patients (90.0%) in group A were labeled as "good" outcome, compared with that of 34 (86.8%) in group B (P = 0.734). Improvement of syringomyelia was also comparable between the groups, which was observed in 35 (87.5%) vs. 33 (86.8%) patients (P = 0.887). The postoperative overall (7.5% vs. 23.7%, P = 0.048) and CSF-related (2.5% vs. 18.4%, P = 0.027) complication rates were significantly lower in group A than group B. CONCLUSIONS: Tonsillectomy with modified reconstruction of the cisterna magna without craniectomy seems to be a safe and effective surgical option to treat adult CM-I patients with syringomyelia, though future well-powered prospective randomized studies are warranted to validate these findings.

2.
Gynecol Endocrinol ; : 1-5, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31902257

RESUMO

Our previous study have demonstrated the elevated cortisol concentration in the follicular fluid (FF) contributed to the insulin resistance of the granulosa cells (GCs) in PCOS, but the complicated cortisol generation mechanisms are still unknown. 11ß-hydroxysteroid type 1(11ß-HSD1) mainly functions as reductase in intact cells, converting cortisone to cortisol. Cortisol and IL-1ß are known to induce 11ß-HSD1 in number of tissues, but few results were obtained in ovarian GCs In this study, FF and GCs from PCOS and non-PCOS patients were collected to study the interaction of cortisol and IL-1ß in 11ß-HSD1 expression. The ELISA and qRT-PCR revealed that the cortisol and IL-1ß concentration in FF and 11ß-HSD1 abundance in GCs were elevated in PCOS patients. By using cultured GCs in vitro, we demonstrated that both cortisol and IL-1ß could stimulate 11ß-HSD1 expression. The induction of 11ß-HSD1 by IL-1ß was further inducted by cortisol, whereas the induction of IL-1ß and IL-6 expression by IL-1ß was completely inhibited by cortisol. In conclusion, cortisol and IL-1ß preformed a synergistically upregulation of 11ß-HSD1 expression in GCs, contributing to the accumulation of cortisol in FF of PCOS patients. This may lead to the metabolic disorders of the ovary.

3.
Traffic Inj Prev ; : 1-6, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31906720

RESUMO

Objective: The primary objective of this study is to explore the effects of the lighting level on nighttime safety of signalized intersections based on conflict models under traffic conditions varying in cycles.Method: Nighttime data were collected from a field study at six signalized intersections in Nanjing, Jiangsu Province in China. Nighttime rear-end conflict models were developed by adopting the generalized linear model (GLM) approach to relate the frequency of rear-end traffic conflicts to lighting level, traffic volume and platoon ratio at the signal cycle level.Results: The final model consisting of all explanatory variables, including lighting level, traffic volume, and platoon ratio demonstrates a better performance of safety evaluation when compared to the model considering traffic volume only and the model with traffic volume and an additional variable of lighting or traffic conditions. Nighttime safety of signalized intersections is expected to improve with larger platoon ratios and higher lighting levels. A potential application of the final model was further explored by benefit-cost analyses. The analyses provided a hypothetical recommended lighting level under various traffic volumes and platoon ratios when safety benefit equals lighting system cost.Conclusions: Nighttime safety can be evaluated using the developed rear-end conflict models, which relate the number of rear-end conflicts to traffic and lighting variables. The number of rear-end conflicts can be calculated by the final conflict model with lighting level, traffic volume, and platoon ratio. The developed model can be potentially applied to provide further insights on the lighting management for intersection safety optimization with traffic conditions varying in signal cycles via vehicle-to-infrastructure (V2I) communications.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31944886

RESUMO

Approximately 20% of COPD patients have been considered to have a 'nonhyperinflator phenotype'. However, this judgment depends on patients making a maximal inspiratory capacity (IC) maneuver at rest, since the IC during exercise is compared to this baseline measurement. We hypothesized that IC maneuvers at rest are sometimes submaximal and tested this hypothesis by measuring IC and associated neural respiratory drive at rest, during inhalation of CO2 and exercise in patients with COPD. Twenty-six COPD patients (age 66±6 years, mean FEV1 40±11% pred) and 39 healthy subjects (age 39±14 years, FEV1 98±12% pred) were studied. IC and the diaphragm electromyogram (EMGdi )associated with it (EMGdi-IC), forced inspiratory vital capacity (FIVC) and its corresponding EMGdi (EMGdi-FIVC) were measured during inhalation of 8% CO2 (8% CO2-92% O2) and room air. Incremental exhaustive cycle ergometer exercise was also performed in both patients with COPD and healthy subjects. IC, EMGdi-IC, FIVC and EMGdi-FIVC during breathing 8% CO2 were significantly greater than those during breathing room air in both patients with COPD and healthy subjects (all p<0.001). EMGdi-IC in patients with COPD constantly increased during exercise from 145 ± 40 µV at rest to 185 ± 52 µV at the end of exercise but change in IC was variable. Neural respiratory drive and IC increased during hypercapnia. Exercise related hypercapnia in patients with COPD raises neural respiratory drives which compensate for IC reduction, leading to underestimation of dynamic hyperinflation measured by IC at rest breathing room air.

6.
Molecules ; 25(2)2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31952118

RESUMO

Spondias pinnata (Linn. f.) Kurz (Anacardiaceae) is widely distributed in tropical Asia, where it is commonly used as a vegetable and fruit, and is attracting increasing research attention. In this study, we investigated the chemical composition and the cytotoxic, antimicrobial, and anti-inflammatory activities of the fruit peel essential oil of S. pinnata (EOSP), which has been consumed as a medicine and condiment in Xishuangbanna, southwest China. A total of 40 components were identified by Gas Chromatography/Mass spectrometry (GC-MS), representing 95.19% of the EOSP, with furfural (17.14%), α-terpineol (13.09%), and ethyl benzoate (9.05%) as the main constituents. EOSP has moderate cytotoxic activity against five cancer cells and obvious antimicrobial activity against five pathogenic strains. In particular, EOSP (Minimal Inhibitory and Fungicidal Concentration, MIC and MFC, 16‒32 µg/mL) showed a 32-times higher inhibition effect against Aspergillus fumigatus than the positive control Tigecycline (MIC and MBC 512‒1024 µg/mL). EOSP also showed strong anti-inflammatory activity by significantly inhibiting nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW 264.7 cell lines at 0.08‱, with no effect on cell viability. These bioactivities of S. pinnata fruit peel validate its traditional uses and suggest that it could be a new source of natural antimicrobial and anti-inflammatory agents for food or medical industries.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31953343

RESUMO

In this study, the mineral-weathering bacterium Pseudomonas azotoformans F77, isolated from the soil of a debris flow area, was evaluated for its weathering activity under direct contact with biotite or without contact. Then, biotite-weathering behaviors of strain F77, its mutants that were created by deleting the gcd and adh genes, which are involved in gluconic acid metabolism and pilus formation, respectively and the double mutant F77ΔgcdΔadh, were compared. The relative gene expression levels of F77 and its mutants F77Δgcd and F77Δadh were also analyzed in the presence of biotite. Direct contact with biotite increased Fe and Al release from the mineral in the presence of F77. All strains had a similar ability to release Fe and Al from the mineral except for F77Δgcd and F77Δadh Mobilized Fe and Al concentrations were decreased by up to 72, 26, and 87% in the presence of F77Δgcd, F77Δadh, and F77ΔgcdΔadh, respectively, compared to those observed in the presence of F77 during the mineral-weathering process. Gluconic acid production was decreased for F77Δgcd and F77ΔgcdΔadh, while decreased cell attachment on the mineral surface was observed for F77Δadh compared to that observed for F77. The F77 genes involved in pilus formation and gluconic acid metabolism showed increased expression levels in the presence of biotite. The results of this study showed the important role of the genes involved in gluconic acid metabolism and pilus formation in mineral weathering by F77 and demonstrated the distinctive effect of these genes on mineral weathering by F77.IMPORTANCE Bacteria play an important role in mineral weathering and soil formation; although the molecular mechanisms underlying the interactions between bacteria and silicate minerals are poorly understood. In this study, the interactions between biotite and the highly effective mineral-weathering bacterium P. azotoformans F77 were characterized. Our results showed that the genes involved in gluconic acid metabolism and pilus formation play important roles in mineral weathering by F77. The presence of biotite could promote the expression of these genes in F77, and a distinctive effect of these genes on mineral weathering by F77 was observed in this study. Our results provide new knowledge and promote better understanding regarding the interaction between silicate minerals and mineral-weathering bacteria and of the molecular mechanisms involved in these processes.

8.
Br J Radiol ; : 20190887, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31904268

RESUMO

OBJECTIVE: In spite of the well-known importance of thalamus in hemifacial spasm (HFS), the thalamic resting-state networks in HFS is still rarely mentioned. This study aimed to investigate resting-state functional connectivity (FC) of the thalamus in HFS patients and examine its association with clinical measures. METHODS: 25 HFS patients and 28 matched healthy controls underwent functional MRI at rest. Using the left and right thalamus as seed regions respectively, we compared the thalamic resting-state networks between patient and control groups using two independent sample t-test. RESULTS: Compared with controls, HFS patients exhibited strengthened bilateral thalamus-seeded FC with the parietal cortex. Enhanced FC between right thalamus and left somatosensory association cortex was linked to worse motor disturbance, and the increased right thalamus-right supramarginal gyrus connection were correlated with improvement of affective symptoms. CONCLUSION: Our findings indicate that the right thalamus-left somatosensory association cortex hyperconnectivity may represent the underlying neuroplasticity related to sensorimotor dysfunction. In addition, the upregulated FC between the right thalamus and right supramarginal gyrus in HFS, is part of the thalamo-default mode network pathway involved in emotional adaptation. ADVANCES IN KNOWLEDGE: This study provides new insights on the integrative role of thalamo-parietal connectivity, which participates in differential neural circuitry as a mechanism underlying motor and emotional functions in HFS patients.

9.
J Am Chem Soc ; 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31940193

RESUMO

The coupling nature of thermoelectric properties determines that optimizing the Fermi level is the priority to achieve a net increase in thermoelectric performance. Conventionally, the carrier concentration is used as the reflection of the Fermi level in the band structure. However, carrier concentration strongly depends upon the material's effective mass, leading to the optimal carrier concentration varies over a large scale for different materials. Herein, inspired by the big data survey, we develop a golden Seebeck coefficient range of 202 - 230 µVK-1 for thermoelectric semiconductors with lattice thermal conductivity of 0.4 - 1.5 Wm-1K-1. When the measured Seebeck coefficient reaches this range, the corresponding figure of merit is maximized. Using this approach, we exemplarily analyze the characteristics of n-type Pb1-xBixSe thermoelectric materials. With detailed electron microscopy and property characterizations, the high densities of dislocations and pores are found to be responsible for a low lattice thermal conductivity. Moreover, Bi substitution significantly tunes the Seebeck coefficient in a wide range. As a result, the Seebeck coefficient of ~-230 µVK-1 in Pb0.98Bi0.02Se is close to the golden range, leading to a figure of merit beyond 1.5. This finding provides an intuitive metric to determine the optimization extent of thermoelectric performance.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31944499

RESUMO

Fully utilizing solar energy for catalysis requires integration of conversion mechanisms and therefore delicate design of catalyst structures and active species. Here, a MOF crystal engineering method was developed to controllably synthesize the copper-ceria catalyst with well-dispersed photoactive Cu-[O]-Ce species. Using preferential CO oxidation as a model reaction, the catalyst showed remarkably efficient and stable photoactivated catalysis, which found practical application in feed gas treatment for fuel cell gas supply. The coexistence of photochemistry and thermochemistry effects contributes to the high efficiency. Our results demonstrate a catalyst design approach with atomic or molecular precision and the combinatorial photoactivation strategy for solar energy conversion.

11.
Zhongguo Zhen Jiu ; 40(1): 75-8, 2020 Jan 12.
Artigo em Chinês | MEDLINE | ID: mdl-31930903

RESUMO

It is well believed that children's spleen is often insufficient, as the structure and qi of spleen-stomach are incomplete. The deficiency of spleen-stomach plays an important role in the occurrence, development and pathogenesis of pediatric diseases. Professor LIU Xiao-ying has inherited the academic thought of NI Zhu-ying, a famous physician of TCM, and proposed the external treatment method of "supporting the middle". This method emphasizes the constant care of the spleen-stomach of children in the aspect of acupoint selection, and adoptes the external treatment of children's tuina, supporting-yang cupping therapy (including warming tuina, warming moxibustion and warm scrapping), acupuncture (including abdominal acupuncture, intradermal needling) to treat children's diseases of spleen, lung, kidney and others, which has achieved satisfactory therapeutic effects.

12.
J Agric Food Chem ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31933359

RESUMO

The strong and inducible cbh1 promoter is most widely used to express heterologous proteins useful in food and feed industries in Trichoderma reesei. Enhancing its ability to direct transcription provides a general strategy to improve protein production in T. reesei. The cbh1 promoter was engineered by replacing eight binding sites of the transcription repressor ACE1 to those of the activators ACE2, Hap2/3/5, and Xyr1, respectively. While changing ACE1 to Hap2/3/5-binding sites completely abolished the transcription ability, replacements with ACE2- and Xyr1-binding sites (designated cbh1pA and cbh1pX promoters, respectively) largely improved the promoter transcription efficiency, as reflected by expression of a reporter gene DsRed. The cbh1pA and cbh1pX promoters were applied to improve secretory expression of a codon-optimized mannanase from Aspergillus niger to 3.6- and 5.0-fold higher, respectively, which has high application potential in feed industry.

13.
Aging (Albany NY) ; 12(1): 193-203, 2020 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-31901899

RESUMO

Our previous studies have demonstrated that interleukin-12p35 knockout (IL-12p35 KO) regulates the progression of various cardiovascular diseases, such as acute cardiac injury and hypertension. The aims of this study were to investigate whether IL-12p35 KO affects aging-related cardiac remodeling and to explore the possible mechanisms. First, the effects of IL-12p35 KO on heart structure and function were detected, and the results showed that IL-12p35 KO exacerbated cardiac remodeling and increased cardiac senescence-related protein levels in aged mice. In addition, whether IL-12p35 KO regulates cardiac senescence-related protein expression, cardiac mitochondrial dysfunction and cardiomyocyte apoptosis was also investigated. IL-12p35 KO increased mitochondrial calcium fluorescence intensity and ROS fluorescence intensity, while it reduced mitochondrial membrane potential. Furthermore, reduced mitochondrial complex (I-IV) activity and ATP levels and increased apoptosis-inducing factor (AIF)-related cardiomyocyte apoptosis were observed in aged IL-12p35 KO mice compared with wild-type mice. Our results demonstrate that aging is aggravated by IL-12p35 KO and that the mechanism may be related to exacerbation of mitochondrial dysfunction and AIF-related cardiomyocyte apoptosis.

14.
Spectrochim Acta A Mol Biomol Spectrosc ; 230: 118025, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31927511

RESUMO

A quinoline-based hydrazone, namely, bis((quinolin-8-yl)methylene)carbonohydrazide (1), has been designed and synthesized, which could be used as a dual probe for selective recognition of Co2+ and Zn2+ by monitoring changes in absorption and fluorescence spectral pattern, respectively. The detection limits of 1 for these two ions are 0.21 and 0.66 µM, respectively. The complex formation between 1 and Co2+/Zn2+ is responsible for the detection process, as confirmed through several spectral methods and DFT calculations. The application of the as-synthesized sensor is demonstrated in Zn2+ imaging in living cells.

15.
World Neurosurg ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31954884

RESUMO

OBJECTIVE: To evaluate the safety and efficacy of an enhanced recovery after surgery (ERAS) program for intraspinal tumors in a single-institutional prospective randomized controlled trial. METHODS: A multimodal and multidisciplinary ERAS protocol for intraspinal tumor surgery was developed. A total of 94 enrolled patients were randomized into two groups: 48 were managed following ERAS protocol (ERAS group), and 46 received conventional perioperative care (control group). The primary endpoint was postoperative length of stay (LOS). The secondary outcomes included postoperative pain score and pain medication use, urinary catheterization, ambulation, mortality, reoperation/readmission rates, complication rates, patient satisfaction, and overall cost. RESULTS: A significant reduction in LOS was achieved in patients undergoing ERAS protocol compared with the controls (5d vs. 8d, P<0.0001). Moreover, patients in ERAS group had better postoperative pain scores (1.0±1.3 vs. 1.9±1.3, P=0.007), decreased use of patient-controlled analgesia (4.2% vs. 19.6%, P=0.020) and oral opioid (37.5% vs. 58.7%, P=0.040), early urinary catheter removal (58.3% vs. 6.5%, P<0.0001), greater ambulation (68.8% vs. 17.4%, P<0.0001), and higher satisfaction scores (91.8±4.4 vs. 88.2±6.8, P=0.022) than control group. There were no deaths or 30-day readmission/reoperation in both groups, nor did the postoperative complication rates differ between groups. CONCLUSIONS: The ERAS protocol for intraspinal tumor surgery seems to be feasible, effective and safe in shortening postoperative LOS, improving postoperative pain control with reduced opioid use, and accelerating functional recovery without increasing rates of complications or reoperation/readmission. Adoption of spine ERAS programs could be encouraged in practice though validation with larger-scale multicenter trials is warranted.

16.
Behav Sleep Med ; 18(1): 1-9, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-30380915

RESUMO

Objective: Mindfulness-based interventions (MBIs) are clinically effective for insomnia, but the research findings have been mixed. This meta-analysis of randomized controlled trials (RCTs) examined the effect of MBIs on insomnia. Method: Both English (PubMed, PsycINFO, Embase, and Cochrane Library databases) and Chinese (WanFang and CNKI) databases were systematically and independently searched. Standardized mean differences (SMDs) and risk ratio (RR) with their 95% confidence intervals (CIs) were calculated using the random effects model. Results: Five RCTs (n = 520) comparing MBIs (n = 279) and control (n = 241) groups were identified and analyzed. Compared to the control group, participants in the MBIs group showed significant improvement in insomnia as measured by the Pittsburgh Sleep Quality Index (n = 247; SMD: -1.01, 95% CI: -1.28 to -0.75, I2 = 0%, p < 0.00001) at post-MBIs assessment. Conclusion: In this comprehensive meta-analysis, MBIs appear to be effective in the treatment of insomnia. Further studies to examine the long-term effects of MBIs for insomnia are needed.

17.
Food Chem ; 311: 125975, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31855777

RESUMO

A modified quick, easy, cheap, effective, rugged, and safe method coupled with ultra-high performance liquid chromatography-tandem mass spectrometry was established for the simultaneous detection of five Alternaria toxins (tenuazonic acid, alternariol, alternariol monomethyl ether, altenuene, and tentoxin) in wolfberry. The sample pretreatment conditions including the dilution solvent, the extraction solvent and the QuEChERS purification parameters were optimized. Detection of the five Alternaria toxins was performed in MRM mode under ESI + conditions. The results showed that the five Alternaria toxins exhibitedgood linearity (1-200 µg/L, with correlation coefficients > 0.999). The limits of detection were 0.07-0.24 µg/kg, and the limits of quantification were 0.32-0.77 µg/kg. The trueness was between 73.8% and 111.5%, and the precision was lower than 10%. The established method was effectively used for the determination of five Alternaria toxins in 155 wolfberry samples from Northwest China.

18.
Insect Sci ; 27(1): 159-169, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29851277

RESUMO

Agasicles hygrophila has been introduced worldwide as a control agent for the invasive weed Alternanthera philoxeroides. However, global warming has potential impact on its controlling efficacy. The aim of this research was to explore the primary factors responsible for the greatly reduced A. hygrophila population in hot summers. To imitate the temperature conditions in summers, different developmental stages of A. hygrophila were treated with high temperatures from 32.5 °C to 45 °C for 1-5 h. Based on the survival rate, the heat tolerance of each developmental stage was ranked from lowest to highest as follows: egg, 1st, 2nd, 3rd instar larva, adult and pupa. Eggs showed the lowest heat tolerance with 37.5 °C as the critical temperature affecting larval hatching. Heat treatment of the A. hygrophila eggs at 37.5 °C for 1 h decreased the hatch rate to 24%. Our results indicated that when compared with the control at 25 °C, 1 h treatment at 37.5 °C prolonged the duration of the egg stage, shortened the duration of oviposition and total longevity, and changed the reproductive pattern of A. hygrophila. The net reproductive rate, intrinsic rate and finite rate were all significantly reduced. The results suggest that low heat tolerance of the eggs was the major factor responsible for the reduction of A. hygrophila populations, and the key temperature was 37.5 °C. Therefore, appropriate measures should be taken to protect eggs in order to maintain the efficacy of A. hygrophila in the biological control of A. philoxeroides in hot summers.


Assuntos
Besouros/fisiologia , Temperatura Alta/efeitos adversos , Termotolerância , Animais , Besouros/crescimento & desenvolvimento , Feminino , Larva/crescimento & desenvolvimento , Larva/fisiologia , Masculino , Óvulo/fisiologia , Pupa/crescimento & desenvolvimento , Pupa/fisiologia , Estações do Ano
19.
Mol Carcinog ; 59(1): 45-55, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31659808

RESUMO

A prostate cancer risk single-nucleotide polymorphism (SNP), rs13426236, is significantly associated with melanophilin (MLPH) expression. To functionally characterize role of the rs13426236 in prostate cancer, we first performed splicing-specific expression quantitative trait loci analysis and refined the significant association of rs13426236 allele G with an increased expression of MLPH splicing transcript variant 4 (V4) (P = 7.61E-5) but not other protein-coding variants (V1-V3) (P > .05). We then performed an allele-specific reporter assay to determine if SNP-containing sequences functioned as an active enhancer. Compared to allele A, allele G of rs13426236 showed significantly higher luciferase activity on the promoter of the splicing transcript V4 (P < .03) but not on the promoter of transcript V1 (P > .05) in two prostate cancer cell lines (DU145 and 22Rv1). Cell transfection assays showed stronger effect of transcript V4 than V1 on promoting cell proliferation, invasion, and antiapoptotic activities. RNA profiling analysis demonstrated that transcript V4 overexpression caused significant expression changes in glycosylation/glycoprotein and metal-binding gene ontology pathways (FDR < 0.01). We also found that both transcripts V4 and V1 were significantly upregulated in prostate adenocarcinoma (P ≤ 2.49E-6) but only transcript V4 upregulation was associated with poor recurrence-free survival (P = .028, hazard ratio = 1.63, 95% confidence interval = 1.05-2.42) in The Cancer Genome Atlas data. This study provides strong evidence showing that prostate cancer risk SNP rs13426236 upregulates expression of MLPH transcript V4, which may function as a candidate oncogene in prostate cancer.

20.
Curr Eye Res ; 45(2): 144-152, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31869261

RESUMO

Purpose: To test the performance of the four tonometers in providing IOP measurements that were free of the effects of corneal biomechanics changes caused by refractive surgery.Methods: Four tonometers were employed to provide IOP measurements for 65 participants who accepted Femtosecond laser-assisted LASIK (FS-LASIK). The measurements included GAT-IOP by the Goldmann Applanation Tonometer, DCT-IOP by the Dynamic Contour Tonometer, Goldmann-correlated IOP (ORA-IOPg) and corneal-compensated IOP (ORA-IOPcc) by the Ocular Response Analyzer, and uncorrected IOP (CVS-IOP) and biomechanically corrected IOP (CVS-bIOP) by the Corvis ST. Statistical analyses were performed to assess the association of the differences in IOP caused by FS-LASIK with central corneal thickness (CCT), mean corneal curvature (Km), age, refractive error correction (REC), optical zone diameter (OZD), ablation zone diameter (AZD), residual stromal bed thickness (RSB) and RSB ratio (RSB/CCT). Multiple linear regression models were constructed to explore factors influencing IOP changes.Results: All four tonometers exhibited significant differences between IOP measurements taken pre and post-surgery except for CVS-bIOP in the low to moderate myopia group (t = 1.602, p = .12). CVS-bIOP, followed by DCT-IOP, provided the best agreement between pre and post-FS-LASIK measurements with the lowest differences in IOP and the narrowest limits of agreement. The pre-post IOP differences were also significantly associated with the reduction in CCT in only GAT-IOP, ORA-IOPg, and CVS-IOP. CVS-bIOP and ORA-IOPcc were the only measurements that were not correlated with CCT, Km or age both before and after FS-LASIK.Conclusions: The biomechanically corrected bIOP from the Corvis ST provided post-FS-LASIK measurements that were in closest agreement with those obtained before surgery. In comparison, GAT-IOP, ORA-IOPg, ORA-IOPcc, and CVS-IOP appeared to be more influenced by the changes in corneal biomechanics caused by FS-LASIK.

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