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1.
Plant J ; 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31529523

RESUMO

The nutritional traits of maize kernels are important for human and animal nutrition, and these traits have undergone selection to meet the diverse nutritional needs of humans. However, our knowledge of the genetic basis of selecting for kernel nutritional traits is limited. Here, we identified both single and epistatic quantitative trait loci (QTLs) that contributed to the differences of oil and carotenoid traits between maize and teosinte. Over half of teosinte alleles of single QTLs increased the values of the detected oil and carotenoid traits. Based on the pleiotropism or linkage information of the identified single QTLs, we constructed a trait-locus network to help clarify the genetic basis of correlations among oil and carotenoid traits. Furthermore, the selection features and evolutionary trajectories of the genes or loci underlying variations in oil and carotenoid traits revealed that these nutritional traits produced diverse selection events during maize domestication and improvement. To illustrate more, a mutator distance-relative transposable element (TE) in intron 1 of DXS2, which encoded a rate-limiting enzyme in the methylerythritol phosphate pathway, was identified to increase carotenoid biosynthesis by enhancing DXS2 expression. This TE occurs in the grass teosinte, and has been found to have undergone selection during maize domestication and improvement, and is almost fixed in yellow maize. Our findings not only provide important insights into evolutionary changes in nutritional traits, but also highlight the feasibility of reintroducing back into commercial agricultural germplasm those nutritionally important genes hidden in wild relatives.

2.
Nat Commun ; 10(1): 3741, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31431616

RESUMO

Clinical experience suggests increased incidences of neonatal jaundice when air quality worsens, yet no studies have quantified this relationship. Here we reports investigations in 25,782 newborns showing an increase in newborn's bilirubin levels, the indicator of neonatal jaundice risk, by 0.076 (95% CI: 0.027-0.125), 0.029 (0.014-0.044) and 0.009 (95% CI: 0.002-0.016) mg/dL per µg/m3 for PM2.5 exposure in the concentration ranges of 10-35, 35-75 and 75-200 µg/m3, respectively. The response is 0.094 (0.077-0.111) and 0.161 (0.07-0.252) mg/dL per µg/m3 for SO2 exposure at 10-15 and above 15 µg/m3, respectively, and 0.351 (0.314-0.388) mg/dL per mg/m3 for CO exposure. Bilirubin levels increase linearly with exposure time between 0 and 48 h. Positive relationship between maternal exposure and newborn bilirubin level is also quantitated. The jaundice-pollution relationship is not affected by top-of-atmosphere incident solar irradiance and atmospheric visibility. Improving air quality may therefore be key to lowering the neonatal jaundice risk.

3.
Nat Genet ; 51(6): 1052-1059, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31152161

RESUMO

Maize is one of the most important crops globally, and it shows remarkable genetic diversity. Knowledge of this diversity could help in crop improvement; however, gold-standard genomes have been elucidated only for modern temperate varieties. Here, we present a high-quality reference genome (contig N50 of 15.78 megabases) of the maize small-kernel inbred line, which is derived from a tropical landrace. Using haplotype maps derived from B73, Mo17 and SK, we identified 80,614 polymorphic structural variants across 521 diverse lines. Approximately 22% of these variants could not be detected by traditional single-nucleotide-polymorphism-based approaches, and some of them could affect gene expression and trait performance. To illustrate the utility of the diverse SK line, we used it to perform map-based cloning of a major effect quantitative trait locus controlling kernel weight-a key trait selected during maize improvement. The underlying candidate gene ZmBARELY ANY MERISTEM1d provides a target for increasing crop yields.


Assuntos
Estudos de Associação Genética , Genoma de Planta , Genômica , Fenótipo , Zea mays/genética , Biologia Computacional/métodos , Genômica/métodos , Sequenciamento de Nucleotídeos em Larga Escala , Endogamia , Anotação de Sequência Molecular , Melhoramento Vegetal , Plantas Geneticamente Modificadas , Polimorfismo de Nucleotídeo Único , Característica Quantitativa Herdável
4.
J Integr Plant Biol ; 60(6): 465-480, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29319223

RESUMO

Flowering time is a trait vital to the adaptation of flowering plants to different environments. Here, we report that CCT domain genes play an important role in flowering in maize (Zea mays L.). Among the 53 CCT family genes we identified in maize, 28 were located in flowering time quantitative trait locus regions and 15 were significantly associated with flowering time, based on candidate-gene association mapping analysis. Furthermore, a CCT gene named ZmCOL3 was shown to be a repressor of flowering. Overexpressing ZmCOL3 delayed flowering time by approximately 4 d, in either long-day or short-day conditions. The absence of one cytosine in the ZmCOL3 3'UTR and the presence of a 551 bp fragment in the promoter region are likely the causal polymorphisms contributing to the maize adaptation from tropical to temperate regions. We propose a modified model of the maize photoperiod pathway, wherein ZmCOL3 acts as an inhibitor of flowering either by transactivating transcription of ZmCCT, one of the key genes regulating maize flowering, or by interfering with the circadian clock.


Assuntos
Relógios Circadianos/genética , Flores/genética , Flores/fisiologia , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Proteínas de Plantas/genética , Zea mays/genética , Modelos Biológicos , Fotoperíodo , Filogenia , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Polimorfismo Genético , Reprodutibilidade dos Testes , Transformação Genética
5.
ANZ J Surg ; 88(4): E252-E256, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27764891

RESUMO

BACKGROUND: Liver metastasis is common in patients with oesophageal cancer. The effect of operative intervention for post-operative solitary liver metastasis from oesophageal squamous cell carcinoma (ESCC) has not previously been examined. This research was to compare the effect of surgery and non-surgical therapy in patients with post-operative solitary liver metastasis from ESCC. METHODS: We retrospectively analysed the clinical data of 69 consecutive patients with solitary hepatic metastasis who had undergone oesophagectomy for ESCC and were subsequently referred to the First Affiliated Hospital of Zhengzhou University from January 2005 to December 2013. The survival rates of the surgical and non-surgical groups were compared. RESULTS: There were 26 patients in the surgical group and 43 patients in the non-surgical group. There was no operative death in the surgical group. Post-operative complications were observed in six patients, and all of these patients recovered after additional treatments. Patients in the surgical group had 1- and 2-year cumulative survival rates of 50.8 and 21.2%, respectively, which were significantly higher than the 31.0 and 7.1% survival rates of patients in the non-surgical group (P < 0.05). In each group, the patients with a disease-free interval (DFI) lasting >12 months had a better survival rate than those with a DFI lasting ≤12 months (all P < 0.05). CONCLUSIONS: Operative intervention is a better treatment choice for patients with post-operative solitary liver metastasis from ESCC, especially for patients with a DFI lasting >12 months. Patients selected for hepatic resection should be considered on an individual basis through a multidisciplinary team of specialists.

6.
Nat Commun ; 8(1): 1874, 2017 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-29187731

RESUMO

Maize was domesticated from lowland teosinte (Zea mays ssp. parviglumis), but the contribution of highland teosinte (Zea mays ssp. mexicana, hereafter mexicana) to modern maize is not clear. Here, two genomes for Mo17 (a modern maize inbred) and mexicana are assembled using a meta-assembly strategy after sequencing of 10 lines derived from a maize-teosinte cross. Comparative analyses reveal a high level of diversity between Mo17, B73, and mexicana, including three Mb-size structural rearrangements. The maize spontaneous mutation rate is estimated to be 2.17 × 10-8 ~3.87 × 10-8 per site per generation with a nonrandom distribution across the genome. A higher deleterious mutation rate is observed in the pericentromeric regions, and might be caused by differences in recombination frequency. Over 10% of the maize genome shows evidence of introgression from the mexicana genome, suggesting that mexicana contributed to maize adaptation and improvement. Our data offer a rich resource for constructing the pan-genome of Zea mays and genetic improvement of modern maize varieties.


Assuntos
Evolução Molecular , Genoma de Planta/genética , Zea mays/genética , Haplótipos
7.
Artigo em Inglês | MEDLINE | ID: mdl-26680686

RESUMO

The golden drum (Chrysochir aurenus) is an important food fish of Indo-West Pacific and China. To study the phylogenetic status, we sequenced the complete mitochondrial genome of C. aurenus. The mitogenome is 16 505 bp in length and composed of 13 protein-coding genes, 2 rRNAs, 22 tRNAs, and a control region. The gene composition and the structural arrangement of C. aurenus complete mtDNA were identical to most of other vertebrates. The phylogenetic analysis using the complete mitochondrial genome revealed that the C. aurenus might be grouped in different genera of Argyrosominae, but not belonged to Otolithinae, which was highly consistent with that based on the morphology. The present study will be helpful for the evolution and conservation genetic studies of C. aurenus.


Assuntos
Genoma Mitocondrial , Perciformes/genética , Animais , Composição de Bases , Códon de Iniciação , Códon de Terminação , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Fases de Leitura Aberta/genética , Perciformes/classificação , Filogenia , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Análise de Sequência de DNA
8.
Sci Rep ; 6: 18936, 2016 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-26729541

RESUMO

Gene expression variation largely contributes to phenotypic diversity and constructing pan-transcriptome is considered necessary for species with complex genomes. However, the regulation mechanisms and functional consequences of pan-transcriptome is unexplored systematically. By analyzing RNA-seq data from 368 maize diverse inbred lines, we identified almost one-third nuclear genes under expression presence and absence variation, which tend to play regulatory roles and are likely regulated by distant eQTLs. The ePAV was directly used as "genotype" to perform GWAS for 15 agronomic phenotypes and 526 metabolic traits to efficiently explore the associations between transcriptomic and phenomic variations. Through a modified assembly strategy, 2,355 high-confidence novel sequences with total 1.9 Mb lengths were found absent within reference genome. Ten randomly selected novel sequences were fully validated with genomic PCR, including another two NBS_LRR candidates potentially affect flavonoids and disease-resistance. A simulation analysis suggested that the pan-transcriptome of the maize whole kernel is approaching a maximum value of 63,000 genes, and through developing two test-cross populations and surveying several most important yield traits, the dispensable genes were shown to contribute to heterosis. Novel perspectives and resources to discover maize quantitative trait variations were provided to better understand the kernel regulation networks and to enhance maize breeding.


Assuntos
Genoma de Planta , Genômica , Característica Quantitativa Herdável , Transcriptoma , Zea mays/genética , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Genes de Plantas , Anotação de Sequência Molecular , Fenótipo
9.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(5): 3633-5, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-26369837

RESUMO

The giant croaker Nibea japonica (Perciformes, Sciaenidae) is an important economic fish distributing in the East China Sea, South China Sea, and Japan southern coast. In this study, the complete mitochondrial genome of N. japonica was firstly determined. It is 16 496 bp-length and consists of 22 tRNA genes, 13 protein-coding genes, two rRNA genes, and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. Phylogenetic analysis revealed that N. japonica, A. amoyensis, and other seven fish first clustered into the Argyrosominae clade. It is consistent with the taxonomic status. Then, the Argyrosominae, Pseudosciaeninae, and Sciaeniae formed the sister group, while the Johniinae became a separate clade, which is inconsistent with the previous phenotypic report. It is suggested that the researches of single gene and taxionomic might lose some significant evolutionary characters. This study will contribute to phyogenetic analysis of the Sciaenidae and the natural resources conservation.


Assuntos
Genoma Mitocondrial , Perciformes/genética , Animais , DNA Mitocondrial/genética , Proteínas de Peixes/genética , Genes de RNAr , Região de Controle de Locus Gênico , Fases de Leitura Aberta/genética , Perciformes/classificação , Filogenia , RNA de Transferência/genética , Sequenciamento Completo do Genoma
10.
Asian Pac J Trop Med ; 7(6): 473-7, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25066397

RESUMO

OBJECTIVE: To analyze expression heterogeneity of Integrin beta 3 (ITGB3) and B-cell lymphoma 2 (BCL-2) in lung adenocarcinoma tissue and adenocarcinoma cell line and further provide theoretical direction for molecular biological research of lung adenocarcinoma. METHODS: Tissue microarray was used to observe relation among expression, heterogeneitpy and clinical characteristics of ITGB3 and BCL-2 in lung cancer. RESULTS: ITGB3 and BCL-2 increased significantly in A549 cells in CAFs group withß-actin as control; the expression level of BCL-2 also increased in ITGB3 transfected cells with GFP plasmid transfected A549 cells as control; immunohistochemistry staining showed that positive rates of ITGB3, ITGB1 and BCL-2 in normal lung tissues were 0, the positive rates in lung adenocarcinoma were 7.04%, 84.51% and 4.23%, respectively; in the results of immunohistochemistry staining, the expression of Girdin protein in lung adenocarcinoma was homogeneous, however protein expression of ITGB3, ITGB1 and BCL-2 showed different patterns in the same location with significant heterogeneity; majority of ITGB3, ITGB1 or BCL-2 positive tissue showed heterogeneity that expression in trailing edge was higher than that of trailing edge in lung adenocarcinoma tissue, the patients with BCL-2 heterogeneity showed higher lymph node metastasis ratio and lower clinical stage (P<0.05); and the expression of ITGB3 and the clinical characteristics of patients were not significant related (P>0.05). CONCLUSIONS: Expression of ITGB3 and BCL-2 in lung adenocarcinoma and adenocarcinoma cell line showed heterogeneity that expression in trailing edge was higher than that of trailing edge, which may play an important role in promoting tumor lymph node metastasis and vascular invasion, and provides a new research direction for exploration of lung adenocarcinoma metastasis mechanism.


Assuntos
Adenocarcinoma/metabolismo , Integrina beta3/análise , Neoplasias Pulmonares/metabolismo , Pulmão/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/análise , Adenocarcinoma/química , Adenocarcinoma de Pulmão , Linhagem Celular Tumoral , Humanos , Integrina beta3/genética , Integrina beta3/metabolismo , Pulmão/química , Neoplasias Pulmonares/química , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Análise Serial de Tecidos , Transfecção
11.
Plasmid ; 67(1): 60-6, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21907733

RESUMO

Hepatocyte nuclear factor-4alpha (HNF-4a) is an important transcription factor in the liver, and regulates a large number of genes involved in many aspects of hepatocyte functions. In this study, a liver-specific transcriptional regulatory element comprised of albumin promoter (ALBp) and alpha-fetoprotein enhancer (AFPe) was obtained and cloned into the plasmid pHNF4sh-CMV(short hairpin RNA targeting HNF4α) with original CMV promoter removed, resulting to pHNF4sh-EP for liver-specific knockdown of HNF4α expression. In an attempt to verify its characteristics, pHNF4sh-EP was transfected to L02, HepG2, and COS1 cell lines in vitro and delivered into mice in vivo. pHNF4sh-CMV and pNCsh-EP were used as controls. For in vitro, the level of HNF4α mRNA and protein was decreased in all cell lines transfected with pHNF4sh-CMV whereas HNF4α mRNA and protein decreasing was only observed in L02 and HepG2 cell lines upon transfection with pHNF4sh-EP, and this decreasing was more significant as compared with pHNF4sh-CMV transfected cells. For in vivo, the decreasing of HNF4α mRNA and protein was observed in both liver and kidney tissues upon transfection with pHNF4sh-CMV. After transfection with pHNF4sh-EP, decreasing of HNF4α mRNA and protein was only found in liver tissue and this decreasing was more significant. No obvious HNF4α mRNA and protein decreasing was detected either in vitro or in vivo after transfected with pNCsh-EP. In conclusion, pHNF4sh-EP could highly-active and liver-specific knockdown of HNF4α expression liver and it will be useful for further study of the funcitions of HNF4α in liver.


Assuntos
Albuminas/genética , Elementos Facilitadores Genéticos/genética , Vetores Genéticos/genética , Fator 4 Nuclear de Hepatócito/metabolismo , Fígado/metabolismo , Regiões Promotoras Genéticas/genética , alfa-Fetoproteínas/genética , Animais , Western Blotting , Células COS , Cercopithecus aethiops , Regulação Neoplásica da Expressão Gênica , Células Hep G2 , Fator 4 Nuclear de Hepatócito/antagonistas & inibidores , Fator 4 Nuclear de Hepatócito/genética , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
12.
Zhonghua Jie He He Hu Xi Za Zhi ; 34(9): 669-72, 2011 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-22177492

RESUMO

OBJECTIVE: To study KRAS and epidermal growth factor receptor (EGFR) mutations in primary non-small cell lung cancer (NSCLC) and their association with the effects of targeted therapy. METHODS: Gene mutations of KRAS and EGFR in both primary tumors and local lymph node metastases from 150 patients with NSCLC were analyzed by direct sequencing. Twelve of the patients were given gefitinib as neoadjuvant therapy after EGFR-TKI sensitive mutations had been detected in biopsies of mediastinal lymph node metastases. RESULTS: Two primary tumors and 10 metastases were identified to have KRAS mutations, while 35 primary tumors and 44 metastases were found to have EGFR mutations. KRAS and EGFR mutation status was different between primary tumors and corresponding metastases in 6.7% (10/150) and 8.67% (13/150) patients, respectively. One patient with no TKI sensitive mutations in the primary tumor showed disease progression with gefitinib therapy. CONCLUSIONS: Our results suggest that a considerable proportion of NSCLC in Chinese patients showed discrepancy in KRAS and EGFR mutation status between primary tumors and corresponding metastases. This observation may have important implications for the use of targeted TKI therapy in the treatment of NSCLC patients.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Receptores ErbB/genética , Neoplasias Pulmonares/genética , Proteínas Proto-Oncogênicas/genética , Proteínas ras/genética , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Metástase Neoplásica , Proteínas Proto-Oncogênicas p21(ras)
13.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 28(6): 1232-6, 2011 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-22295720

RESUMO

Research about proteomics is of great significance. Two-dimensional electrophoresis (2-DE) is a core technology of proteomics research, which is used for analysis of the protein extracted from cell, tissue and other sam-nology of proteomics research, which is used for analysis of the protein extracted from cell, tissue and other samples. In recent years, 2-DE combined with mass spectrum (MS) technology is widely used to identify differentialples. In recent years, 2-DE combined with mass spectrum (MS) technology is widely used to identify differential protein, to screen tumor markers, to detect drug targets and so on. Proteomics research has become key technology,protein, to screen tumor markers, to detect drug targets and so on. Proteomics research has become key technology, with its high throughput, high resolution and repeatability, and is widely used in various fields, particularly in bio-with its high throughput, high resolution and repeatability, and is widely used in various fields, particularly in biomedical research. We provided here a short review about the application development of 2-DE, especially its contribution on biological medicine.


Assuntos
Pesquisa Biomédica/métodos , Eletroforese em Gel Bidimensional/tendências , Proteômica/métodos , Biomarcadores Tumorais/análise , Humanos , Neoplasias/diagnóstico , Proteoma/análise , Viroses/diagnóstico , Viroses/metabolismo
14.
Dig Dis Sci ; 55(8): 2373-80, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20512414

RESUMO

BACKGROUND: The prognosis of patients with hepatitis B virus (HBV)-associated acute on chronic liver failure (ACLF) is extremely poor. AIM: This study was designed to evaluate the efficacy and safety of nucleoside analogue treatment of patients with HBV-associated ACLF. METHODS: We used a retrospective review of eligible patients from April 2006 to December 2008. Eligible subjects received 0.5 mg entecavir daily until October 2009 (group A), 100 mg lamivudine daily until October 2009 (group B), or no nucleoside analogue (group C). The primary endpoints were three-month survival and the rate of recurrence of HBV-associated ACLF. The secondary endpoints were HBV DNA levels, liver function, the model of end-stage liver disease (MELD) score, and adverse events. RESULTS: A total of 104 consecutive patients were recruited, and 33, 34, and 37 patients were randomly allocated to groups A, B, and C, respectively. Although no significant difference in three-month survival was observed, levels of HBV DNA and rates of recurrence of HBV-associated ACLF were lower. Liver function and MELD score were not significantly improved despite significantly reduced HBV DNA levels. CONCLUSIONS: These data indicated that nucleoside analogue treatment did not improve the short-term prognosis of patients with HBV-associated ACLF although it was efficacious and safe in the management of HBV DNA levels. Intriguingly and importantly, continuous nucleoside analogue treatment can significantly reduce the rate of recurrence, which might be indicative of the further benefit of long-term survival.


Assuntos
Guanina/análogos & derivados , Hepatite B Crônica/complicações , Lamivudina/uso terapêutico , Falência Hepática/tratamento farmacológico , Adulto , Antivirais/uso terapêutico , DNA Viral/sangue , Feminino , Guanina/uso terapêutico , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
15.
Appl Opt ; 48(11): 1998-2005, 2009 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-19363536

RESUMO

We report the design and testing of a novel linear scanning periodic optical delay line (ODL) by use of a helicoid reflective mirror based on a tilted parabolic generatrix that was driven by an electrical motor for a periodic change in the optical path length of the reflected light beam. The divergence and pulse front distortion of the optical beam reflected by the helicoid reflective mirror were simulated based on differential geometry. With a round-trip pass arrangement, a scanning range of delay time as large as 100 ps was obtained by spinning the helicoid reflective mirror with a pitch distance of 7.5 mm. This periodic ODL was used in an optical second-harmonic generation autocorrelator to test the linearity and temporal resolution in comparison with the autocorrelation signal obtained using an ODL structured with a motorized linear translation stage. Experiments demonstrate that our helicoid optical delay device may provide exceptional performance for optical interference, high-resolution terahertz time-domain spectroscopy, and general optical pump-probe experiments.

17.
Contemp Clin Trials ; 29(5): 696-704, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18554990

RESUMO

The purpose of this paper is to evaluate the efficacy and safety of recombinant human hepatocyte growth factor (rh-HGF) for liver failure (LF) using meta-analysis of data from the literature involving available randomized controlled trials of rh-HGF plus comprehensive therapy (CT) compared with that of CT alone (Therapy I versus II) in treating LF. We searched the Cochrane Library, MEDLINE, EMBASE, CBMdisc, and CNKI as well as employing manual searches. Based on our search criteria, we found 21 trials, involving 5902 patients. Our results showed that Therapy I, compared with therapy II, significantly reduced the overall mortality (RR=0.62; 95% CI, 0.59-0.66; p=0.0001). Compared to two clinical types of LF (acute and acute-on-chronic), therapy I perhaps had significant effect on mortality due to sub-acute LF, RR and 95% CI were 0.76 [0.65, 0.89], 0.66 [0.60, 0.74], and 0.58 [0.53, 0.64], respectively. Additionally, there was a reduction in mortality of patients that had evidence for an early stage of LF compared to the two other clinical stages of LF (Middle and Advanced); RR and 95% CI were 0.34 [0.24, 0.49], 0.49 [0.44, 0.55], and 0.87 [0.82, 0.93], respectively. No serious adverse events were reported. We conclude that Therapy I may reduce mortality in LF, especially in sub-acute LF and the early stage of LF. However, considering the strength of the evidence, additional randomized controlled trials are needed before Therapy I can be recommended routinely.


Assuntos
Fator de Crescimento de Hepatócito/uso terapêutico , Falência Hepática/tratamento farmacológico , Garantia da Qualidade dos Cuidados de Saúde , Proteínas Recombinantes/uso terapêutico , Intervalos de Confiança , Humanos , Risco
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