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1.
Biomed Res Int ; 2021: 6645252, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33969122

RESUMO

Objectives: Handgrip strength (HS) is a risk factor of all-cause mortality and cardiovascular diseases. However, the influencing factors and mechanisms contributing to this correlation remain unclear. Therefore, we aimed to explore factors related to HS and investigated the mechanism underlying its risk predictive value. Methods: This was a prospective, cross-sectional study. One hundred forty-five participants were recruited from December 2019 to November 2020. HS was measured using a hydraulic hand dynamometer and adjusted for body mass index (HSBMI) and body surface area (HSBSA). Body composition was assessed via bioimpedance spectroscopy. Physical fitness was measured using a cardiopulmonary exercise test system. Univariate, multiple linear regression analyses and receiver operator characteristic curve (ROC) were conducted to evaluate the associations between various participant characteristics and HS. Results: The average participant age was 21.68 ± 2.61 years (42.8% were male). We found positive correlations between HSBMI/HSBSA and VO2max, VEmax, Loadmax, and METmax in both sexes (p < 0.05). Lean-tissue, protein, total water, and inorganic salt percentages were positively correlated, and fat percentage was negatively correlated with HSBMI in men and with HSBMI and HSBSA in women (p < 0.05). Multiple regression revealed that VO2max was independently associated with HSBSA in both sexes (ß = 0.215, 0.173; 95%confidence interval [CI] = 0.032 - 0.398, 0.026-0.321; p = 0.022, 0.022, respectively) and independently associated with HSBMI in women (ß = 0.016, 95%CI = 0.004 - 0.029, p = 0.011). ROC analysis showed that HSBMI and HSBSA can moderately identify normal VO2max in men (area under curve [AUC] = 0.754, 0.769; p = 0.002, 0.001, respectively) and marginally identify normal VO2max in women (AUC = 0.643, 0.635; p = 0.029, 0.042, respectively). Conclusions: BMI- and BSA-adjusted HS could serve as indicators of physical health, and HSBSA may moderately reflect cardiorespiratory fitness levels in healthy young adults, particularly in males. Clinical trials registry site and number: China Clinical Trial Center (ChiCTR1900028228).

2.
J Stroke Cerebrovasc Dis ; 30(7): 105807, 2021 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-33895428

RESUMO

BACKGROUND: Robot-assisted gait training has been confirmed to have beneficial effect on the rehabilitation of stroke patients. An exoskeleton robot, named BEAR-H1, is designed to help stroke patients with walking disabilities. METHODS: 17 subjects in experimental group and 15 subjects in control group completed the study. The experimental group received 30 minutes of BEAR-H1 assisted gait training(BAGT), and the control group received 30 minutes of conventional training, 5 times/week for 4weeks. All subjects were evaluated with 6-minute walk test (6MWT), Fugl-Meyer Assessment for lower extremity (FMA-LE), Functional Ambulatory Classification (FAC), Modified Ashworth Scale (MAS), and gait analysis at baseline and after 4 weeks intervention. RESULTS: The improvements of 6MWT, FMA-LE, gait speed, cadence, step length and cycle duration in BAGT group were more noticeable than in the control group. However, there was no difference in the assessment of MAS between two groups. CONCLUSIONS: Our results showed that BAGT is an effective intervention to improve the motor and walking ability during 4 weeks training for subacute stroke patients.

3.
Oral Oncol ; : 105290, 2021 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-33888406

RESUMO

BCOR-rearranged sarcoma is the third common subtype of undifferentiated small round cell sarcoma and was first recognized in 2012 from bone sarcoma with a similar morphological manifestation with undifferentiated round cell but totally different molecular hallmark from Ewing sarcoma. Primary BCOR-rearranged sarcomas were mostly found in bone and soft tissue and were sporadically reported in viscera involving kidney, which add difficulties in differential diagnosis. Here we present a 21 years old male patient with BCOR-CCNB3 in neck, which first diagnosed as thyroid cancer.

4.
Zhongguo Zhen Jiu ; 41(3): 252-6, 2021 Mar 12.
Artigo em Chinês | MEDLINE | ID: mdl-33798305

RESUMO

OBJECTIVE: To compare the efficacy of interactive scalp acupuncture, scalp acupuncture alone and scalp acupuncture plus cognitive training for cognitive dysfunction after stroke. METHODS: A total of 660 patients with cognitive dysfunction after stroke were randomly divided into an interactive scalp acupuncture group (218 cases, 18 cases dropped off), a scalp acupuncture group (220 cases, 20 cases dropped off) and a scalp acupuncture plus cognitive training group (222 cases, 22 cases dropped off). All the patients were treated with routine medication and exercise rehabilitation training. The interactive scalp acupuncture group was treated with scalp acupuncture on the parietal midline, and contralateral anterior parietal temporal oblique line and posterior parietal temporal oblique line at the same time of cognitive training; the scalp acupuncture group was treated with scalp acupuncture alone, and the scalp acupuncture plus cognitive training group was treated with scalp acupuncture and cognitive training in the morning and afternoon respectively. All the treatments were given once a day, 6 times a week for 8 weeks. Montreal cognitive assessment (MoCA) scale score was used to evaluate the cognitive function before treatment, 4 weeks and 8 weeks into treatment. RESULTS: Compared before treatment, the total score of MoCA was increased after 4-week treatment and 8-week treatment in the three groups (P<0.01), and the score in the interactive scalp acupuncture group was higher than that in the scalp acupuncture group and the scalp acupuncture plus cognitive training group (P<0.05, P<0.01). Compared before treatment, each item score of MoCA was increased after 8-week treatment in the three groups (P<0.01), and the score in the interactive scalp acupuncture group was higher than that in the scalp acupuncture group and the scalp acupuncture plus cognitive training group (P<0.01). Except for the attention, the remaining items scores of MoCA in the scalp acupuncture plus cognitive training group were higher than those in the scalp acupuncture group (P<0.01). CONCLUSION: The interactive scalp acupuncture could significantly improve the cognitive function in patients with cognitive dysfunction after stroke, and the efficacy is superior to scalp acupuncture alone and scalp acupuncture plus cognitive training.


Assuntos
Terapia por Acupuntura , Disfunção Cognitiva , Reabilitação do Acidente Vascular Cerebral , Acidente Vascular Cerebral , Pontos de Acupuntura , Disfunção Cognitiva/etiologia , Disfunção Cognitiva/terapia , Humanos , Couro Cabeludo , Acidente Vascular Cerebral/complicações , Resultado do Tratamento
5.
Emerg Infect Dis ; 27(6)2021 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-33834988

RESUMO

In October 2020, highly pathogenic avian influenza A(H5N8) viruses were detected in 2 dead swans in Inner Mongolia, China. Genetic analysis showed that the H5N8 isolates belong to clade 2.3.4.4b and that the isolates cluster with the H5N8 viruses isolated in Eurasia in the fall of 2020.

6.
BMC Ophthalmol ; 21(1): 116, 2021 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-33663431

RESUMO

BACKGROUND: To evaluate the 12-month efficacy and safety of intravitreal conbercept for myopic choroidal neovascularization (CNV). METHODS: A retrospective, observational study. Thirty-four eyes of 34 pathologic myopic patients with CNV were treated with intravitreal conbercept (IVC) 0.5 mg with a follow up of 12 months. After the first injection, administration of conbercept followed a pro re nata (PRN) regimen. Outcomes included best corrected visual acuity (BCVA), central retinal thickness (CRT), CNV size, the total number of treatments, and adverse events. RESULTS: The mean patient age was 55.88 ± 16.17 years, and the mean eye spherical equivalent was - 8.72 ± 3.75 D. The mean number of IVC over 12 months was 2.12 ± 0.69. Overall, best-corrected visual acuity(BCVA)improved from 0.86 ± 0.33 logMAR at baseline to 0.44 ± 0.32 logMAR at month 12 (p < 0.001), mean improvement of vision was 4.12 ± 2.69 lines. Mean central retinal thickness reduced from 285.9 ± 104.6 µm at baseline to 192.1 ± 97.5 µm at month 12 (p < 0.001). Mean CNV size decreased from 0.52 ± 0.38 mm2 at baseline to 0.31 ± 0.19 mm2 at 12 months (p < 0.05). All the 34 eyes had reduced or stable size of CNV. Thirty-two eyes (94.12 %) showed the absence of CNV leakage at the end of the study period. No severe systemic or ocular adverse events were observed. CONCLUSIONS: Intravitreal conbercept 0.5 mg was safe and effective for treatment of myopic CNV over 12 months in a real-world setting.

7.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 46(1): 39-46, 2021 Jan 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-33678635

RESUMO

OBJECTIVES: To explore the effect of etomidate on the neuronal activity of ventral thalamic reuniens nucleus and the underlying mechanisms. METHODS: Whole-cell patch clamp method was used to explore the effect of etomidate on the activity of ventral thalamic reuniens neurons in the acute brain slices obtained from 4-5 weeks old C57BL/6J mice. The electrophysiological characteristics of ventral thalamic reuniens neurons were recorded in the current clamp mode, and then the effects of etomidate (0.5, 2.0, 8.0 µmol/L etomidate groups) and intralipid (intralipid group) on the discharge frequency and membrane potential of ventral thalamic reuniens neurons were recorded. During the experiment, the ventral thalamic reuniens neuron firing rates (RNFRs) were recorded as FB, FD and Fw before, after administration, and after elution; and the membrane potential was recorded as MPB and MPD before, after administration. The chlorine channel of gamma-amino butyric acid Type A (GABAA) receptor was blocked with 100 µmol/L picrotoxin (PTX). The RNFRs were recorded as FBS, FETO and FETO+PTX before, after perfusing etomidate with sub-anesthesia concentration (0.5 µmol/L) and after perfusing both PTX and etomidate. RESULTS: In the intralipid group, there was no significant difference among the FB, FD and Fw (P>0.05). But in the etomidate groups (0.5, 2.0, 8.0 µmol/L), the FD was less than the FB, there was significant difference (all P<0.01); the Fw was higher than the FD, there was significant difference (all P<0.05). Moreover, there was significant difference in the inhibitory degree of the RNFRs between the 0.5 µmol/L etomidate group and the 8.0 µmol/L etomidate group (P<0.05). In the experiment to explore the mechanism of etomidate (0.5 µmol/L), the FETO was compared with the FBS, there was significant difference (P<0.01); but when the FETO+PTX was compared with the FBS, there was no significant difference (P>0.05). CONCLUSIONS: Etomidate can inhibit the activity of ventral thalamic reuniens neurons in concentration-dependent manner, and which is reversible. Etomidate with sub-anesthetic concentration inhibits the activity of ventral thalamic reuniens neurons via targeting the GABAA receptor.


Assuntos
Etomidato , Animais , Etomidato/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Neurônios , Técnicas de Patch-Clamp , Receptores de GABA-A
8.
Artigo em Inglês | MEDLINE | ID: mdl-33705312

RESUMO

Sparse representation has achieved great success across various fields including signal processing, machine learning and computer vision. However, most existing sparse representation methods are confined to the real valued data. This largely limit their applicability to the quaternion valued data, which has been widely used in numerous applications such as color image processing. Another critical issue is that their performance may be severely hampered due to the data noise or outliers in practice. To tackle the problems above, in this work we propose a robust quaternion valued sparse representation (RQVSR) method in a fully quaternion valued setting. To handle the quaternion noises, we first define a new robust estimator referred as quaternion Welsch estimator to measure the quaternion residual error. Compared to the conventional quaternion mean square error, it can largely suppress the impact of large data corruption and outliers. To implement RQVSR, we have overcome the difficulties raised by the noncommutativity of quaternion multiplication and developed an effective algorithm by leveraging the half-quadratic theory and the alternating direction method of multipliers framework. The experimental results show the effectiveness and robustness of the proposed method for quaternion sparse signal recovery and color image reconstruction.

9.
Reprod Sci ; 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33725311

RESUMO

Nerve growth factor (NGF) plays an important role in regulating the hypothalamus-pituitary-gonadal (HPG) axis. However, the effects of NGF on spermatogenesis remain unclear. This study aimed to assess the potential application of NGF with nasal delivery on spermatogenesis in azoospermic mice. We established a model with azoospermia induced by a single intraperitoneal (i.p.) injection of busulfan. NGF pre-encapsulated with liposomes (25, 50, and 100 µg/kg) was delivered via internasal administration. Three weeks after busulfan injection, NGF treatments were performed twice a week for 8 weeks; the change of sperm quality, testis and epididymis histopathology, and androgenic hormone were analyzed to evaluate sperm regeneration. Furthermore, 30 mg/kg busulfan injection caused severe testicular atrophy of the seminiferous tubules, characterized by a loss of spermatogenic elements and sperms. NGF with nasal administration could significantly upregulate the markers expressing meiotic spermatogonia (Stra8) and spermatocytes (SYCP3), restore spermatogenesis, and improve sperm quality in busulfan-treated mice by increasing the secretion of sexual hormones. The convenient and noninvasive nasal delivery of NGF may be a new potential therapy for spermatogenesis via activating the HPG axis and elevating androgenic hormones. This study opened a new horizon for NGF application in reproductive endocrine.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33656532

RESUMO

CONTEXT: The role of immune-related genes (IRGs) in thyroid cancer dedifferentiation and accompanying immune exhaustion remains largely unexplored. OBJECTIVE: To construct a significant IRG-based signature indicative of dedifferentiation and immune exhaustion in thyroid cancer. DESIGN AND SETTINGS: One exploratory cohort and two validation cohorts were used to identify stably dysregulated IRGs in dedifferentiated thyroid cancer (DDTC) and to obtain independent risk factors for dedifferentiation. The IRGs formed a gene signature, whose predictive value was tested by the receiver operating characteristic curve. Correlations between the signature and differentiation-related genes, immune checkpoints, and prognosis were analyzed. Gene set enrichment analyses were performed to identify related signaling pathways. RESULTS: Four IRGs, PRKCQ, PLAUR, PSMD2, and BMP7, were found to be repeatedly dysregulated in DDTC, and they formed an IRG-based signature with a satisfactory predictive value for thyroid cancer dedifferentiation. Correlation analyses revealed that immune checkpoints were closely related to the four IRGs and the IRG-based signature, which was significantly associated with the histological subtype (P = 0.026), lymph node metastasis (P = 0.001) and BRAFV600E mutation (P < 0.001). The downregulated expression of PRKCQ shortened the disease-free survival for patients with thyroid cancer. Furthermore, we identified several signaling pathways inherently associated with the IRG-based signature. CONCLUSIONS: This study suggests that IRGs participate in the dedifferentiation and immune exhaustion process of thyroid cancer and are potential biomarkers for DDTC.

11.
Arch Microbiol ; 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33646337

RESUMO

The medicinal fungus Sanghuang produces diverse bioactive compounds and is widely used in Asian countries. However, little is known about the genes and pathways involved in the biosynthesis of these active compounds. Based on our previous study providing Sanghuangporus vaninii genomic information, the transcriptomes of MY (mycelium), OY (1-year-old fruiting bodies), and TY (3-year-old fruiting bodies) were determined in this study. A significant number of genes (4774) were up- or downregulated between mycelium and fruiting bodies, but only 1422 differentially expressed genes were detected between OY and TY. 138 genes encoding P450s were identified in the fungal genome and grouped into 25 P450 families; more than 64% (88) of the genes were significantly differentially expressed between the mycelium and fruiting body, suggesting that these P450s are involved in fungal sexual development. Importantly, the expression of genes involved in bioactive compound (triterpenoids, polysaccharides, and flavonoids) biosynthesis in asexual (cultured with solid and liquid media) and sexual stages was explored and combined with transcriptome and quantitative PCR analyses. More genes involved in the biosynthesis of bioactive compounds were expressed more highly in mycelium than in fruiting bodies under liquid medium culture compared with solid medium culture, which was consistent with the yields of different bioactive compounds, suggesting that liquid fermentation of S. vaninii Kangneng can be used to obtain these bioactive compounds. A comprehensive understanding of the genomic information of S. vaninii will facilitate its potential use in pharmacological and industrial applications.

12.
Medicine (Baltimore) ; 100(10): e24940, 2021 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-33725855

RESUMO

ABSTRACT: Although non-alcoholic fatty liver disease (NAFLD) is strongly associated with type 2 diabetes mellitus (T2DM), the diagnosis of NAFLD for T2DM patients remains a challenge.This study aimed to investigate the prevalence and risk factors for the NAFLD in T2DM outpatients.This is a retrospective, cross-sectional study that included 2405 T2DM patients treated and admitted for glucose control into the Endocrinology Department of our hospital from April 2017 to March 2019. Using strict exclusion criteria, the target patients were screened and divided into two groups: NAFLD patients (study group) and non-NAFLD patients (control group). Subsequently, 34 factors were compared between the two groups. Furthermore, multivariate analysis of the NAFLD risk factors was performed using logistic regression. Finally, the diagnostic significance of individual biochemical predictors, as well as the combined predictive indicator (CPI), for NAFLD was estimated using receiver operating characteristic (ROC) curve analysis.In this study, the overall prevalence of NAFLD in T2DM patients was 58.67%. Of the target patients, 17 factors were identified by univariate analysis to be associated with NAFLD, and 8 factors were found to be significant predictors for NAFLD using binary logistic regression modeling. Furthermore, the CPI and C-Peptide represent high diagnostic value for NAFLD in T2DM patients.This study provides a more comprehensive risk factor analysis for NAFLD in T2DM patients. These data can be used to provide timely diagnosis and effective management of NAFLD.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Adulto , Idoso , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/diagnóstico , Hepatopatia Gordurosa não Alcoólica/etiologia , Prevalência , Estudos Retrospectivos , Medição de Risco/estatística & dados numéricos , Fatores de Risco
14.
Vaccines (Basel) ; 9(2)2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33579020

RESUMO

Infectious bursal disease (IBD), an immunosuppressive disease of young chickens, is caused by infectious bursal disease virus (IBDV). Novel variant IBDV (nVarIBDV), a virus that can evade immune protection against very virulent IBDV (vvIBDV), is becoming a threat to the poultry industry. Therefore, nVarIBDV-specific vaccine is much needed for nVarIBDV control. In this study, the VP2 protein of SHG19 (a representative strain of nVarIBDV) was successfully expressed using an Escherichia coli expression system and further purified via ammonium sulfate precipitation and size-exclusion chromatography. The purified protein SHG19-VP2-466 could self-assemble into 25-nm virus-like particle (VLP). Subsequently, the immunogenicity and protective effect of the SHG19-VLP vaccine were evaluated using animal experiments, which indicated that the SHG19-VLP vaccine elicited neutralization antibodies and provided 100% protection against the nVarIBDV. Furthermore, the protective efficacy of the SHG19-VLP vaccine against the vvIBDV was evaluated. Although the SHG19-VLP vaccine induced a comparatively lower vvIBDV-specific neutralization antibody titer, it provided good protection against the lethal vvIBDV. In summary, the SHG19-VLP candidate vaccine could provide complete immune protection against the homologous nVarIBDV as well as the heterologous vvIBDV. This study is of significance to the comprehensive prevention and control of the recent atypical IBD epidemic.

15.
Accid Anal Prev ; 152: 105979, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33548586

RESUMO

Due to immaturity in their physical and cognitive development, children are particularly vulnerable to road traffic injuries as pedestrians. Child pedestrian injury primarily occurs in urban areas, with a significant share at crosswalks. The aim of this study is to explore whether an intervention programme based on the theory of "behaviour spectrums" can improve the street-crossing skills of primary school children. Children were recruited near a local primary school through invitation letters and were randomly divided into two groups: a control group (n = 10, no intervention) and an experimental group (n = 10, intervention). The children in the experimental group received 30-45 min of training. The child participants were asked to wear an eye tracker and performed a crossing test in a real-world street environment; in this test, they were required to successively pass through an unsignalised intersection, an unsignalised T-intersection and a signalised intersection on a designated test route. A high-definition camera was used to record the children's crossing behaviour, and the Tobii Pro Glasses 2 eye tracker was used to derive indicators of the children's visual behaviour in the areas of interest (AOIs) in the street. The evaluation was conducted on children's crossing behaviour in the control group (which received no intervention) and the experimental group (tested at two time points after the intervention: children tested immediately after the intervention and children retested one month after the intervention). The results showed that compared with the control group, the children in the experimental group no longer focused on the small area around the body (e.g., the zebra crossing area) and the area in front of the eyes (e.g., the sidewalk area), which increased their visual attention to the traffic areas on the left and right sides of the zebra crossing; thus, unsafe crossing behaviour was reduced in the experimental group. Compared with the experimental group immediately after the intervention, the intervention effect on some indicators showed a significant weakening trend in the retest of the experimental group one month later. Overall, the results show that an intervention programme based on the theory of "behaviour spectrums" can improve children's crossing skills. This study provides valuable information for the development and evaluation of intervention programmes to improve children's street-crossing skills.

16.
BMC Musculoskelet Disord ; 22(1): 203, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33602187

RESUMO

BACKGROUND: The surgical treatment of acetabular fracture has adverse outcomes and high risk, and minimally invasive method is a good way to reduce complications and improve hip joint function. This study is to investigate the treatment of certain acetabular fractures primarily involving the anterior column and quadrilateral plate using a limited pararectus approach and the anatomical plates. METHODS: A consecutive cohort of 17 patients with anterior displaced acetabular fractures were managed operatively with a limited approach and the anatomical plates. Ten patients had anterior column fractures, 1 patient had anterior wall fracture, 4 patients had transverse fractures and 2 patients had anterior column with posterior hemi-transverse fractures. The inferior half of the pararectus approach was adopted to open the medial window and to access the anterior column and the quadrilateral plate. The anatomical plates were used for internal fixation. Residual displacements were assessed on the postoperative CT scans using a standardized digital method. The surgical details, hip functional outcomes, and complications were noted. RESULTS: All of the patients were operated using the limited pararectus approach and the anatomical plates successfully. The mean operative time and blood loss were 90.9 min and 334.1 ml, respectively. The average postoperative residual gap and step displacement on CT were 2.9 mm and 0.7 mm, respectively. The radiological outcome was estimated according to the Matta score, ten of the cases were graded anatomical, six were graded imperfect, and one was graded poor. Follow up averaged 15 months. Functional outcomes were excellent for nine, good for six, and fair for two. It was noted that one case of peritoneal injury was repaired intraoperatively. CONCLUSIONS: The limited pararectus approach with the advantages of less trauma, direct exposure to the anterior column and quadrilateral plate. The anatomical plates can fit with the surface of the acetabulum, which saves the time of remodeling plates during operation and facilitate fracture reduction. The combination approach can be a good choice for limited surgery of displaced anterior acetabular fractures especially involving the quadrilateral plate.

17.
World Neurosurg ; 2021 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-33561552

RESUMO

BACKGROUND: This study explored diffusion tensor imaging (DTI) and diffusion tensor tractography (DTT) to assess the prognosis of thoracolumbar spinal cord injury (SCI). METHODS: Twenty patients with acute traumatic thoracolumbar complete SCI (T1-L1, American Spinal Injury Association Impairment Scale [AIS] grade A) underwent conventional magnetic resonance imaging and DTI examinations. DTI measured the fractional anisotropy (FA) and apparent diffusion coefficient adjacent to the lesion epicenter. DTT was used to detect the white matter fiber morphology and measure the imaginary white matter fiber volume and connection rates of fiber tractography (CRFT). The patients' neurological functions were evaluated by the AIS grades. RESULTS: At the final-follow-up, among the 20 patients with AIS grade A, 15 maintained the AIS grade (group A), and 5 patients showed improvement of AIS grade (group B). Group A's mean FA value was significantly lower than that of group B, whereas the mean apparent diffusion coefficient value among the 2 groups showed no significant difference. The white matter fibers of most patients in group A were completely ruptured (11/15), but the white matter fibers of all patients in group B were retained in different number (5/5). The mean CRFT of group B was significantly higher than that of group A (P < 0.05). The improvement of AIS grade was slightly positively correlated with FA values and highly positively correlated with CRFT. CONCLUSIONS: The prognosis of complete thoracolumbar SCI may be related to the FA value and the CRFT. The application of DTI and DTT may optimize the diagnosis of thoracolumbar SCI.

18.
Food Chem ; 345: 128840, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33601658

RESUMO

A simple, rapid and accurate detection method for the authentication of animal species is urgently required in the food detection field. The present study established a horse-specific polymerase chain reaction integrated with a lateral flow sensor assay (Horse-PCR-LFS) for the rapid detection of horse meat. In this test, a cytb gene sequence of horse was amplified using PCR, the PCR amplicon was checked with the lateral flow sensor assay, and the result of the sensor can be read within 2-3 min by the naked eye. The detection limit of the test was up to 0.01% horse meat in artificially adulterated meat mixtures, the assay also successfully detected horse DNA in various commercial food samples. As a rapid and user-friendly molecular detection tool, this test provides an accurate detection format for the identification of horse and offers solutions to problems related to animal meat adulteration and animal-origin food safety and traceability.


Assuntos
DNA/análise , Cavalos/genética , Produtos da Carne/análise , Carne/análise , Reação em Cadeia da Polimerase/métodos , Animais , DNA/metabolismo , Sondas de DNA/metabolismo , Análise de Alimentos , Reprodutibilidade dos Testes
19.
Mol Cell Biochem ; 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33599894

RESUMO

MiR-200a acts as a key role in tumor malignant progression. This work purposed to assess the function of miR-200a in Wilm's tumor. Based on bioinformatics analysis, the expression, prognostic value and related pathways of miR-200a and CDC7 (a potential downstream molecule of miR-200a) in Wilm's tumor were analyzed. qRT-PCR was conducted to confirm the miR-200a level in Wilm's tumor cells. The luciferase reporter assay was carried out to verify the binding of miR-200a to 3'-UTR of CDC7. Then, the impacts of miR-200a and CDC7 on cell viability and apoptosis were measured using CCK-8 and flow cytometry assays. Also, western blot was applied to measure the expression of CDC7 as well as Wnt/ß-catenin signaling pathway-related proteins and apoptosis proteins. Herein, we revealed that miR-200a was lowly expressed in Wilm's tumor tissues and cells and the low miR-200a expression is closely bound up with death and poor outcomes. Moreover, miR-200a directly targeted and inhibited CDC7 in Wilm's tumor cells. Biological function experiments illustrated that overexpression of miR-200a reduced the viability and elevated the apoptosis of Wilm's tumor cells, while overexpression of CDC7 reversed the inhibitory impact of miR-200a on cell viability and the promoting impact of miR-200a on cell apoptosis. Besides, we revealed that miR-200a/CDC7 axis can decrease the expression of ß-Catenin, Cyclin D1 and C-Myc as well as the phosphorylation of GSK-3ß, thus inhibiting the Wnt/ß-catenin signaling pathway. Furthermore, blocking the Wnt/ß-catenin signaling pathway caused an increase on cell apoptosis, while overexpression of CDC7 can reverse these impacts. Collectively, miR-200a/CDC7 axis involved in regulating the malignant phenotype of Wilm's tumor through Wnt/ß-catenin signaling pathway, which provides a theoretical basis for targeted molecular therapy of Wilm's tumor.

20.
Artigo em Inglês | MEDLINE | ID: mdl-33430091

RESUMO

With the development of Internet technology, the speed of information dissemination and accelerated updates result in frequent discussion of topics and expressions of public opinion. In general, multi-dimensional discussion topics related to the same event are often generated in the network, and the phenomenon of multi-dimensional public opinion polarization is formed under the mutual influence of groups. This paper targets the phenomenon of multi-dimensional public opinion polarization under topic-derived situations as the research object. Firstly, this paper identifies the factors influencing multi-dimensional public opinion polarization, including the mutual influence of different topic dimensions and the interaction of viewpoints within the same topic. Secondly, the topic correlation coefficient is introduced to describe the correlation among topics in different dimensions, and the individual topic support degree is used to measure the influence of topics in different dimensions and that of information from external intervention on individual attitudes. Thirdly, a multi-dimensional public opinion polarization model is constructed by further integrating multi-dimensional attitude interaction rules. Finally, the influence of individual participation, topic status, topic correlation coefficient and external intervention information on the multi-dimensional public opinion polarization process is analyzed through simulation experiments. The simulation results show that:(1) when there is a negative correlation between multi-dimensional topics, as the number of participants on different dimensional topics becomes more consistent, the conflict between multi-dimensional topics will weaken the polarization effect of overall public opinion. However, the effect of public opinion polarization will be enhanced alongwith the enhancement in the confidence of individual opinions. (2) The intervention of external intervention information in different dimensions at different times will further form a multi-dimensional and multi-stage public opinion polarization, and when the multi-dimensional topics are negatively correlated, the intervention of external intervention information will have a stronger impact on the multi-dimensional and multi-stage public opinion polarization process. Finally, the rationality and validity of the proposed model are verified by a real case.


Assuntos
Atitude , Opinião Pública , Simulação por Computador , Disseminação de Informação
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