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1.
Chem Commun (Camb) ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34643625

RESUMO

In this work, we report a much simpler and low-cost method to prepare main-chain-type semifluorinated alternating copolymers by the formation of a halogen bond (XB) complex between α,ω-diiodoperfluoroalkanes and amines/halide salts. It is interesting that the terminal iodine functional group of the polymer chains is easily lost in the amine-promoted system, while the loss can be significantly reduced by adding a small amount of water. Importantly, the system promoted by halide salts can ensure complete retention of the iodine functional group. Overall, the establishment of this method provides a new strategy for designing smart fluoropolymer materials in a green and environmentally friendly facile manner under irradiation with visible light at room temperature.

2.
Iran J Immunol ; 18(3): 221-225, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34596591

RESUMO

BACKGROUND: Purpuric nephritis is the most common secondary glomerular disease in childhood. Its prevalence in children has been steadily rising in recent years. OBJECTIVE: To explore the characteristics and pathogenesis of peripheral blood lymphocyte subsets and immune function changes in children with Henoch-Schonlein purpura nephritis. METHODS: The study included 104 children with Henoch-Schonlein purpura, divided into nephritis (HSPN) group (68 cases) and non-nephritis (NHSPN) group (36 cases), and 15 normal children were included as the control group. The rate-scatter turbidimetric method was utilized to determine the immunoglobulins IgA, IgG, IgM, C3 and C4, and the flow cytometry analysis technique was employed to detect the levels of lymphocyte subsets such as CD3+, CD4+, CD8+, CD4+/CD8+, CD19+, NK, etc. Results: Compared with the control group, the CD3+, CD4+, CD8+ and NK cell levels of peripheral blood mononuclear cells in the HSPN group and the NHSPN group significantly decreased (P<0.05), and the CD19+ level significantly elevated (P<0.05); whereas the HSPN group had a more significant change than the NHSPN group (P<0.05). Compared with the control group, the serum immunoglobulin IgA and IgG of the HSPN group and the NHSPN group significantly increased, and the IgM, C3, and C4 significantly decreased (P<0.05); while the HSPN group had a more significant change than the NHSPN group (P<0.05). CONCLUSION: Immune dysfunction in children with HSPN is specifically manifested as low cellular immune function, which leads to an increased secretion of inflammatory mediators, activates B cells, and further increases the secretion of immunoglobulins, leading to the occurrence of small vasculitis.

3.
Exp Biol Med (Maywood) ; : 15353702211038511, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34514884

RESUMO

In breast cancer, tumor-associated macrophages with activated phenotypes promote tumor invasion and metastasis. The more aggressive mesenchymal-like breast cancer cells have a selective advantage, skewing macrophages toward the more immunosuppressive subtype. However, the mechanism underlying this shift is poorly understood. Cyclin D1b is a highly oncogenic variant of cyclin D1. Our previous study showed that non-metastatic epithelial-like breast cancer cells were highly metastatic in vivo when cyclin D1b was overexpressed. The present study determined whether cyclin D1b contributed to the interaction between breast cancer cells and macrophages. The results showed that cyclin D1b promoted the invasion of breast cancer cells in vitro. Specifically, through overexpression of cyclin D1b, breast cancer cells regulated the differentiation of macrophages into a more immunosuppressive M2 phenotype. Notably, tumor cells overexpressing cyclin D1b activated macrophages and induced migration of breast cancer cells. Further investigations indicated that SDF-1 mediated macrophage activation through breast cancer cells overexpressing cyclin D1b. These results revealed a previously unknown link between aggressive breast cancer cells and Tumor-associated macrophages, and highlighted the importance of cyclin D1b activity in the breast cancer microenvironment.

4.
Turk J Med Sci ; 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34392671

RESUMO

OBJECTIVE: To investigate the similarities and differences of renal clinical and renal pathology between IgA nephropathy (IgAN) and IgA Vasculitis Nephritis (IgAVN) in children. METHODS: A total of 237 children with IgAN and 190 children with IgAVN were included. The general conditions, clinical characteristics, final diagnosis, clinical and pathological classification of the children were intercepted at the time of admission, and the retrospective comparative analysis was carried out. RESULTS: The results showed that the median course of disease in IgAN group was longer than that in IgAVN group (P=0.02). Patients with IgAN had a significantly higher duration of infection than the patients with IgAVN (P=0.03). The white blood cell count (WBC), hemoglobin (HGB) in IgAN group were significantly lower than that in IgAVN group (P=0.02). The serum creatinine in IgAN group was higher than that in IgAVN group (P=0.02). Patients with IgAN and IgAVN had statistically significant differences in pathological typing between clinical types: hematuria and proteinuria, nephrotic syndrome and chronic nephritis (P=0.004). CONCLUSION: The clinical manifestations of IgAN and IgAVN were similar, but the onset of IgAN was hidden and the clinical manifestations were relatively serious. Renal pathology was mainly glomerulosclerosis and renal tubular atrophy. IgAVN was characterized by acute onset and good renal function. Renal pathology was dominated by endothelial hyperplasia and crescent formation. These differences did not support the hypothesis that the two diseases are the same.

5.
Molecules ; 26(13)2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34202760

RESUMO

A phytochemical investigation of the leaves of the medicinal plant Isodon rubescens led to the isolation of the two new degraded abietane lactone diterpenoids rubesanolides F (1) and G (2). Their structures were elucidated based on the analyses of the HRESIMS and 1D/2D NMR spectral data, and their absolute configurations were determined by ECD spectrum calculations and X-ray single crystal diffraction methods. Compounds 1 and 2, with a unique γ-lactone subgroup between C-8 and C-20, were found to form a carbonyl carbon at C-13 by removal of the isopropyl group in an abietane diterpene skeleton. Rubesanolide G (2) is a rare case of abietane that possesses a cis-fused configuration between rings B and C. The two isolates were evaluated for their biological activities against two cancer cell lines (A549 and HL60), three fungal strains (Candida alba, Aspergillus niger and Rhizopus nigricans) and three bacterial strains (Escherichia coli, Staphylococcus aureus and Bacillus subtilis).


Assuntos
Abietanos , Anti-Infecciosos , Antineoplásicos Fitogênicos , Bactérias/crescimento & desenvolvimento , Fungos/crescimento & desenvolvimento , Isodon/química , Lactonas , Neoplasias/tratamento farmacológico , Folhas de Planta/química , Células A549 , Abietanos/química , Abietanos/isolamento & purificação , Abietanos/farmacologia , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Células HL-60 , Humanos , Lactonas/química , Lactonas/isolamento & purificação , Lactonas/farmacologia , Neoplasias/metabolismo , Neoplasias/patologia
6.
BMC Cardiovasc Disord ; 21(1): 291, 2021 06 11.
Artigo em Inglês | MEDLINE | ID: mdl-34116640

RESUMO

BACKGROUND: Few studies have compared bioelectrical body and visceral fat indices with anthropometric measures, or evaluated their optimal cutoffs in relation to hypertension among Asians. We compared the efficiencies of bioelectrical indices (percentage of body fat, PBF; visceral fat area, VFA) with anthropometric measures (body mass index, BMI; waist-hip ratio, WHR) for hypertension and re-evaluated the optimal cutoffs of each index by age and gender. METHODS: We conducted a cross-sectional survey among 8234 adults for health examination. PBF, VFA, BMI, WHR, and data on hypertension and behaviors were collected. Receiver operating characteristic (ROC) curve and areas under curves (AUCs) were used to analyze the efficiencies of the indices for hypertension, optimal cutoffs were estimated using the Youden index. RESULTS: A total of 8234 individuals aged 21-91 with median age 44 (interquartile range [IQR] 33-56) years were included and 40.56% were men. The overall prevalence of hypertension was 27.47%. The studied indices were all associated with hypertension in all age-specific groups both among men and women except for WHR in 21-29 years old men and PBF in in 21-29 years old women. Among males, there were no statistical differences in powers of four indices for hypertension in all age-specific groups, except for 40-49 years, in which WHR was better than VFA. Among females, no differences were found among the indices in 30-39 and 70-79 years groups, while WHR was the best in 21-29 years group, VFA was better than PBF in 30-39 and 50-59 years groups, BMI was better than PBF and WHR in 60-69 years group. The optimal cutoffs of PBF, VFA, BMI and WHR ranged from 23.9 to 28.7%, 86.4 to 106.9cm2, 23.5 to 27.1 kg/m2, 0.92 to 0.96 across the age categories in males, and 32.8 to 36.3%, 75.9 to 130.9cm2, 21.9 to 26.4 kg/m2, 0.84 to 0.95 across the age categories in females, respectively. CONCLUSIONS: The obesity indices' efficiencies for hypertension varied by age and gender, and their cutoff values varied across the age categories and gender. Specific indices and cutoffs based on person's age and gender should be used to identify individuals with hypertension.


Assuntos
Adiposidade , Antropometria , Hipertensão/diagnóstico , Gordura Intra-Abdominal/fisiopatologia , Obesidade/diagnóstico , Adulto , Distribuição por Idade , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Pressão Sanguínea , China/epidemiologia , Estudos Transversais , Impedância Elétrica , Feminino , Inquéritos Epidemiológicos , Humanos , Hipertensão/epidemiologia , Hipertensão/fisiopatologia , Masculino , Pessoa de Meia-Idade , Obesidade/epidemiologia , Obesidade/fisiopatologia , Valor Preditivo dos Testes , Prevalência , Fatores de Risco , Distribuição por Sexo , Fatores Sexuais , Adulto Jovem
7.
J Ultrasound Med ; 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33818784

RESUMO

OBJECTIVES: A new method based on the adhesion of SonoVue to plasmids was assessed to achieve targeted gene delivery into the vascular endothelium. METHODS: pEGFP-Salusin-α and pcDNA3.1-Salusin-α plasmids were transfected into the arterial endothelium of different rabbit groups. Western blotting was performed to analyze the expression of EGFP and salusin-α in the common carotid arteries of rabbits from different groups, and ELISA was performed to detect plasma salusin-α levels in rabbits from each group; simultaneously, blood parameters of different groups of rabbits were measured. RESULTS: Green fluorescence was observed in the right common carotid artery of rabbits transfected with pEGFP-Salusin-α, but not in the endothelial cells of not-transfected control rabbits. The expression of salusin-α in the transfected animals was higher than that in the control not-transfected animals (P < 0.05). In rabbits transfected with pcDNA3.1-Salusin-α plasmid, salusin-α expression was higher than in the not-transfected control animals (P < 0.05). However, there was no significant difference in plasma salusin-α levels between transfected animals and controls (P > 0.05). Blood parameters were also measured in both groups. CONCLUSIONS: Our data confirm the establishment of a new method using SonoVue for targeted gene delivery into the arterial endothelium. Our study outcomes propose a new method of intervention in atherosclerosis and a new tool for targeted gene delivery.

8.
Endokrynol Pol ; 71(6): 573-574, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33125698

RESUMO

Not required for Clinical Vignette.

10.
J Orthop Surg Res ; 14(1): 103, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30975166

RESUMO

BACKGROUND: Osteosarcoma was locally aggressive and frequently metastasizes to the lung. However, the etiology of osteosarcoma was unknown. Thus, exploring the mechanisms behind the occurrence of osteosarcoma was important for its prediction and prevention. To investigate the usefulness of mammalian Eps15 homology domain 1 (EHD1) as a prognostic marker for osteosarcoma, the expression of EHD1 in 57 osteosarcoma patients was measured using immunohistochemistry techniques and correlated with the clinicopathological features of patients. METHODS: Correlations of EHD1 expression levels with clinicopathological features of patients were assessed using the Pearson χ2 test for categorical variables and the Student t test for continuous variables. Cumulative disease-free survival (DFS) curves and overall survival (OS) curves were plotted using the Kaplan-Meier method, and the relationship between each of the variables and survival was assessed by log-rank tests using univariate analysis. Subsequently, the parameters were tested using the multivariate Cox proportional hazards model, which was used to identify independent variables for predicting survival. EHD1 expression [P = 0.020; HR, 5.582; 95% confidence intervals (CI), 1.314-23.72] was an independent prognostic indicator of DFS in osteosarcoma patients; tumor size and EHD1 expression of osteosarcomas were independent prognostic indicators of OS in osteosarcoma patients. RESULTS: EHD1 protein expression was a positive expression in examined tumor tissues. The median OS time of patients with high expression of EHD1 was 46.8 months (95% CI, 29.8-63.8 months), and the median OS time of patients with low expression of EHD1 was 58.8 months (95% CI, 31.6-86.0 months). The prognosis for patients with low expression of EHD1 in osteosarcomas was significantly better than that for patients with high expression of EHD1 (log-rank test, P = 0.019). CONCLUSION: The expression of EHD1 was negatively correlated with DFS and OS of osteosarcoma patients; therefore, the expression of EHD1 is a prognostic marker for prediction and prevention of osteosarcomas.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/biossíntese , Neoplasias Ósseas/metabolismo , Regulação Neoplásica da Expressão Gênica , Osteossarcoma/metabolismo , Proteínas de Transporte Vesicular/biossíntese , Proteínas Adaptadoras de Transdução de Sinal/genética , Adolescente , Adulto , Neoplasias Ósseas/genética , Neoplasias Ósseas/mortalidade , Criança , Pré-Escolar , Intervalo Livre de Doença , Feminino , Humanos , Masculino , Osteossarcoma/genética , Osteossarcoma/mortalidade , Taxa de Sobrevida/tendências , Proteínas de Transporte Vesicular/genética , Adulto Jovem
11.
FASEB J ; 33(7): 8008-8021, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30913399

RESUMO

Schwann cells are the main supportive cells of the peripheral nerves. Schwann cells suffer inhibition of autophagy under hyperglycemia treatment in diabetic peripheral neuropathy (DPN). However, the exact mechanism is still not fully elucidated. We first observed the decrease of autophagy markers (LC3-II/LC3-I, P62) in the sciatic nerves of diabetic mice vs. normal mice, accompanied with the loss of myelinated nerve fibers and abnormal myelin sheath. In line with this, LC3-II/LC3-I and P62 were also significantly reduced in high glucose-treated rat Schwann cell 96 (RSC96) cells compared with normal glucose-treated cells. Furthermore, we found that trichostatin A [an inhibitor of histone deacetylase (HDAC)] evidently improved LC3-II/LC3-I in high glucose-treated RSC96 cells, without an effect on P62 expression. Again, HDAC1 and HDAC5 were revealed to be increased in RSC96 cells stimulated with high glucose. Inhibition of HDAC1 but not HDAC5 by small hairpin RNA vector enhanced LC3-II/LC3-I in high glucose-cultured RSC96 cells. In addition, LC3-II conversion regulators [autophagy-related protein (Atg)3, Atg5, and Atg7] were detected in high glucose-treated and HDAC1-knockdown RSC96 cells, and Atg3 was proven to be the key target of HDAC1. The presuppression of Atg3 offset the improvement of LC3-II/LC3-I resulting from HDAC1 inhibition in high glucose-treated RSC96 cells. The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway was activated in RSC96 cells treated with high glucose, which was indicated by increased STAT3 phosphorylation. Blocking STAT3 phosphorylation by chemical inhibitor AG490 induced HDAC1 down-regulation followed by increases in Atg3 and LC3-II/LC3-I. Interestingly, we also found that AG490 treatment enhanced P62 expression in high glucose-stimulated RSC96 cells. Taken together, our findings demonstrate that hyperglycemia inhibits LC3-II/LC3-I in an HDAC1-Atg3-dependent manner and decreases P62 expression in an HDAC-independent manner via the JAK-STAT3 signaling pathway in the Schwann cells of DPN.-Du, W., Wang, N., Li, F. Jia, K., An, J., Liu, Y., Wang, Y., Zhu, L., Zhao, S. Hao, J. STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy.


Assuntos
Autofagia/efeitos dos fármacos , Neuropatias Diabéticas/metabolismo , Glucose/farmacologia , Histona Desacetilase 1/fisiologia , Processamento de Proteína Pós-Traducional , Fator de Transcrição STAT3/metabolismo , Células de Schwann/efeitos dos fármacos , Animais , Proteínas Relacionadas à Autofagia/antagonistas & inibidores , Proteínas Relacionadas à Autofagia/biossíntese , Proteínas Relacionadas à Autofagia/genética , Biomarcadores , Linhagem Celular , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Neuropatias Diabéticas/patologia , Técnicas de Silenciamento de Genes , Histona Desacetilase 1/antagonistas & inibidores , Histona Desacetilase 1/genética , Histona Desacetilases/genética , Histona Desacetilases/fisiologia , Ácidos Hidroxâmicos/farmacologia , Camundongos , Proteínas Associadas aos Microtúbulos/biossíntese , Proteínas Associadas aos Microtúbulos/genética , Bainha de Mielina/patologia , Fibras Nervosas Mielinizadas/patologia , Peptídeo Sintases/antagonistas & inibidores , Peptídeo Sintases/biossíntese , Peptídeo Sintases/genética , Fosforilação , Interferência de RNA , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/farmacologia , Ratos , Células de Schwann/metabolismo , Nervo Isquiático/metabolismo , Nervo Isquiático/patologia , Tirfostinas/farmacologia , Regulação para Cima
12.
Biomed Pharmacother ; 109: 1346-1350, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30551385

RESUMO

Interleukin 8 (IL-8) is an important pro-inflammatory cytokine that recruits neutrophil to the areas of inflammation and has been implicated in myocardial ischemia reperfusion injury (MIRI). This study aimed to apply IL-8 targeted myocardial contrast echocardiography (MCE) to evaluate MIRI in rabbits. MCE imaging with IL-8 targeted microbubbles (MBIL-8) and control microbubbles (MBc) was performed in 40 Japanese white rabbits after brief proximal left anterior descending (LAD) partial occlusion for 30 min and subsequent reperfusion for 30 min, 60 min, 120 min and 180 min. Electrocardiogram and regional wall motion were assessed during occlusion and reperfusion. MCE demonstrated that IL-8 level rapidly increased in reperfused myocardial tissue and reached the peak after 120 min of reperfusion and lasted to 180 min of reperfusion. ELISA showed that the tendency of MCE data to change with reperfusion time was the same as that of IL-8 content. Taken together, these results suggest that targeted MCE with IL-8 antibody provides a new approach to noninvasive evaluation of MIRI using ultrasound imaging techniques.


Assuntos
Interleucina-8/metabolismo , Isquemia Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/metabolismo , Miocárdio/metabolismo , Animais , Meios de Contraste/metabolismo , Ecocardiografia/métodos , Masculino , Microbolhas , Infarto do Miocárdio/metabolismo , Reperfusão Miocárdica/métodos , Coelhos
13.
Biomed Res Int ; 2018: 8973986, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30105261

RESUMO

Inhibiting vascular endothelial foam is the focus of clinical attention. Using SonoVue (an ultrasound contrast agent), the salusin-α gene was transfected into the arterial intima of an atherosclerotic rabbit model induced by a high-fat diet in this study. Subsequently the model of blood lipid indexes, the pathological structure of the intima, and changes in molecules regulating atherosclerosis were investigated. The high-density lipoprotein C and apolipoprotein A values in the salusin-α gene overexpression (P) group were higher than those in the salusin-α gene interference (RP) group (P < 0.05), whereas the total cholesterol, low-density lipoprotein C, and apolipoprotein B values were reversed. Rabbits in the P group showed significantly thinner vascular intimal thickness than that of other experimental groups (P < 0.05). The expression of positive regulators of atherosclerosis (ABCA1, ABCG1) was higher in the P group than that in the RP group (P < 0.05), and the opposite effect was observed for negative regulators (ACAT1, CD36). Thus, our results showed that the overexpression of salusin-α gene inhibited the proliferation of the vascular intima, thereby throwing some light on understanding the mechanism how salusin-α gene expression interfered with the foaming of vascular intimal cells.


Assuntos
Aterosclerose/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Túnica Íntima/metabolismo , Animais , Hiperplasia , Lipoproteínas LDL , Modelos Animais , Coelhos
14.
PLoS One ; 12(7): e0180514, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28692662

RESUMO

Mycoplasma infection has been reported in immunocompromised cancer patients; nevertheless, it is not clear if persistent Mycoplasma infection could facilitate the proliferation of cancer cells in immunocompromised organisms. The aim of this study was to examine the relationship between persistent Mycoplasma infection and malignant transformation in an immunodeficient host model. Immunodeficient mouse model was established using cyclophosphamide and mice gastric mucosal cells were infected with Mycoplasma penetrans (Mpe). After 18 weeks, mice were sacrificed and gastric mucosal Mpe infected cells were identified by fluorescence in situ hybridization (FISH). Moreover, pathological and ultrastructural changes in mice gastric mucosa were evaluated and the expression of multiple proto-oncogenes was examined by Western blot. Our data show that Mpe infection was detected in the blood of immunodeficient mice and Mpe persistent infection in mice gastric mucosa was confirmed by FISH. There were pathological and ultrastructural malignant transformation occurred in the gastric mucosa of infected mice compared to control mice. Mpe infected mice showed lower expression of p53 and p21 and higher H-ras expression compared to the control group. Moreover, expression of NF-κB p65 subunit increased in Mpe infected mice, similar to the TNF-α expression. Bax expression in gastric mucosa of Mpe infected mice was lower while Bcl-2 expression was higher than in the uninfected control group. Collectively these data demonstrate that persistent Mpe infection is associated with aberrant expression of multiple proto-oncogenes in gastric mucosa of immunodeficient mice which potentially facilitate the malignant transformation.


Assuntos
Transformação Celular Neoplásica/patologia , Mucosa Gástrica/microbiologia , Mucosa Gástrica/patologia , Infecções por Mycoplasma/microbiologia , Infecções por Mycoplasma/patologia , Mycoplasma penetrans/fisiologia , Animais , Apoptose , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Feminino , Mucosa Gástrica/ultraestrutura , Camundongos Endogâmicos C57BL , Camundongos SCID , Infecções por Mycoplasma/diagnóstico , Mycoplasma penetrans/ultraestrutura , NF-kappa B/metabolismo , Transdução de Sinais , Fator de Necrose Tumoral alfa/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Proteína X Associada a bcl-2/metabolismo , Proteínas ras/metabolismo
15.
J Drug Target ; 24(2): 102-10, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26302771

RESUMO

BACKGROUND: In this work, we investigated the functional role of microRNA 137 (miR-137) in regulating osteosarcoma both in vitro and in vivo. METHODS: Quantitative RT-PCR was used to examine the gene expressions of miR-137 in osteosarcoma cell lines and osteosarcoma tumors. 143B and Saos-2 cells were infected with lentivirus expressing miR-137 mimics (miR-137-mimic) to ectopically upregulate miR-137. In vitro cancer proliferation and migration were examined by MTT assay and transwell assay, respectively. Viral infected Saos-2 cells were also subcutaneously inoculated into null mice to evaluate the effect of miR-137 upregulation on in vivo tumor growth. The interaction between miR-137 and its downstream target, FXYD6, was evaluated by dual-luciferase reporter assay and quantitative real-time PCR. FXYD6 was then subsequently upregulated in osteosarcoma cells to evaluate its effect on miR-137 regulation in osteosarcoma. RESULTS: We found that miR-137 was significantly downregulated in both osteosarcoma cell lines and osteosarcoma tumors. Lentiviral infection of miR-137-mimic upregulated miR-137 gene expression, reduced in vitro proliferation and migration and inhibited in vivo osteosarcoma tumor growth. FXYD6 was verified to be directly interacting with miR-137, and its subsequent upregulation reversed the inhibitory effect of miR-137 upregulation in osteosarcoma. CONCLUSION: We revealed novel functional role of miR-137 in osteosarcoma regulation, likely through FXYD6 binding.


Assuntos
Neoplasias Ósseas/genética , Movimento Celular/genética , Proliferação de Células/genética , Regulação para Baixo/genética , Canais Iônicos/genética , MicroRNAs/genética , Osteossarcoma/genética , Animais , Linhagem Celular Tumoral , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Células HEK293 , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Regulação para Cima/genética
16.
JRSM Short Rep ; 4(10): 2042533313476690, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24319575

RESUMO

OBJECTIVE: Perineural invasion of cholangiocarcinoma happens in the early stage of the disease but is often not recognized until its later stages. Research about the behaviour and mechanism of perineural invasion by cholangiocarcinoma is urgently needed for a useful new model. The aim of this work is to establish a novel model to address the problem. DESIGN: Neural cells and cholangiocarcinoma cells were co-cultured to mimic the neurotropic invasion of cholangiocarcinoma. SETTING: Human embryonic stem cells were induced to form neural cells by glial cell-derived neurotropic factor and retinoic acid; neural cells and cholangiocarcinoma cells were co-cultured in Transwell chamber. PARTICIPANTS: Human embryonic stem cells and cholangiocarcinoma cells were applied. MAIN OUTCOME MEASURES: Paired t-test was used to compare the counts of penetrating cholangiocarcinoma cells in co-culture and control group. RESULTS: Formation of neurospheres and neural-like cells were observed following induction at 24 and 48 h, respectively; synapses were viewed to protrude from neural-like cell bodies after incubation for 96 h. Forty-eight hours after incubation, immunocytochemical staining of the cells showed that synaptophysin and glial fibrillary acidic protein were expressed in the neuron-like cells and gliocytes-like cells, respectively. The cholangiocarcinoma cells that had penetrated through the Matrigel/polyethylene terephthalate membrane from the upper chamber to the lower chamber of the Transwell in the co-culture group were significantly more numerous than those in the control group (68 ± 8.3/field versus 46 ± 5.7/field, P < 0.05). CONCLUSION: The novel model is a valuable tool to study the perineural invasion of cholangiocarcinoma.

17.
J Agric Food Chem ; 61(19): 4533-8, 2013 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-23647238

RESUMO

Few data were available on the interactions between polyphenols and polysaccharides. The effects of the chemical structure of polyphenols on their interactions with oat ß-glucan were analyzed. Ultrafiltration was applied to determine the adsorption capacities of polyphenols into oat ß-glucan. Hydroxylation favored the adsorption of flavonoids with three or fewer hydroxyl groups but deteriorated those with four or more hydroxyl groups. Among flavonoid isomers, the adsorption capacities increased in the order flavonol > flvaone > flavanone > isoflavone. Glycosylation exerted complicated influences on the adsorption capacities of flavonoids into oat ß-glucan. In most cases, methylation and methoxylation of phenolic acids lowered their adsorption capacities into oat ß-glucan. Esterification of gallic acid weakened its adsorption capacity into oat ß-glucan, whereas o-coumaric acid presented higher adsorption capacity into oat ß-glucan than p- and m-coumaric acids. Galloylation improved the adsorption capacities of catechins into oat ß-glucan.


Assuntos
Polifenóis/química , beta-Glucanas/química , Catequina/química , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos/química , Esterificação , Ácido Gálico/química , Glicosilação , Interações Hidrofóbicas e Hidrofílicas , Hidroxilação , Estrutura Molecular , Relação Estrutura-Atividade
19.
J Agric Food Chem ; 59(19): 10737-46, 2011 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-21892831

RESUMO

Few data are available about the effects of complexation of polyphenols with polysaccharide on their bioavailability. The complex of tea polyphenols (TP) with oat ß-glucan was characterized by ultraviolet-visible spectrometry, Fourier transform infrared spectrometry, differential scanning calorimetry, atomic force microscopy, and solid-state (13)C NMR spectroscopy. The results indicated that the bonds which governed the interaction between TP and oat ß-glucan were strong hydrogen bonds. The in vitro antioxidant activity of TP, ß-glucan, their complex, and physical mixture was assessed using four systems, namely, DPPH(•), OH(•), and O(2)(•-) scavenging activities and reducing power. The complexation and blending of TP and ß-glucan exhibited different impacts on the index of in vitro and in vivo antioxidant capacities. In the concentration range of 0.5-2.5 mg mL(-1), the complex had highest O(2)(•-) scavenging activity, whereas the highest OH(•) scavenging activity was found with the physical mixture. For antioxidant testing in vivo, there was no significant difference between the complex and the physical mixture in terms of glutathione peroxidase activity and levels of malondialdehyde and total antioxidant capacity in serums. However, the complex exhibited much higher activities of superoxide dismutase and glutathione peroxidase in livers than the physical mixture. The present study provided a deeper understanding of the influence of molecular interaction between TP and oat ß-glucan on their antioxidant activities.


Assuntos
Antioxidantes/química , Avena/química , Polifenóis/química , Chá/química , beta-Glucanas/química , Animais , Antioxidantes/farmacologia , Fenômenos Químicos , Interações Medicamentosas , Feminino , Glutationa Peroxidase/metabolismo , Ligação de Hidrogênio , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Camundongos , Polifenóis/administração & dosagem , Polifenóis/farmacologia , Relação Estrutura-Atividade , Superóxido Dismutase/metabolismo , beta-Glucanas/administração & dosagem , beta-Glucanas/farmacologia
20.
Ultrasound Med Biol ; 36(11): 1876-83, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20888684

RESUMO

Here we report a new, simple and efficient method by using ultrasound and a microbubble agent (SonoVue) for delivering a gene to balloon-injured carotid arteries for restenosis prophylaxis. The tissue factor pathway inhibitor-2 (TFPI-2) has been shown to inhibit the postinjury intimae hyperplasia in atherosclerotic vessels. New Zealand white rabbits were divided into 4 groups with 14 in each, a treatment control for balloon injury, a gene vehicle control, a gene delivery of TFPI-2 without using ultrasound and a gene delivery of TFPI-2 using ultrasound. After four weeks, the injured artery neointimal proliferation was significantly lower in the TFPI-2 group with ultrasound than the control groups (p < 0.01) according to the measurement of the mean luminal diameters by B-mode ultrasonography. The ratio of intimal/media area and the stenosis rate in the gene delivery facilitated by ultrasound were significantly lower than those of the nonultrasound gene delivering method (p < 0.01).


Assuntos
Arteriosclerose/terapia , Terapia Genética/métodos , Glicoproteínas/metabolismo , Fosfolipídeos/farmacologia , Hexafluoreto de Enxofre/farmacologia , Túnica Íntima/lesões , Ultrassom , Análise de Variância , Angioplastia com Balão , Animais , Meios de Contraste/farmacologia , Técnicas de Transferência de Genes , Hiperplasia , Processamento de Imagem Assistida por Computador , Técnicas Imunoenzimáticas , Plasmídeos , Coelhos
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