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1.
Artigo em Inglês | MEDLINE | ID: mdl-32608528

RESUMO

BACKGROUND: Asthma is a serious global health problem, severely affecting the lives of sufferers and their families. An exceptionally hygienic home and reduced microbial exposure can aggravate the incidence of childhood asthma. METHODS: Specific-pathogen-free BALB/c mice were pre-treated with bacterial lysate (BL; 1 mg/kg) as a high microbial load maternal mouse model, and then, the offspring mice were established as an allergic airway disease (AAD) model. The expression levels of TLR2, TLR4, and HDAC9 in the mother's intestine and the offspring's lungs were detected. Relevant indicators of regulatory T cells (Tregs) were identified in the mother and offspring mice. The changes in the expression of Th1-, Th2-, Th9-, and Th17-related cytokines in the offspring mice were evaluated among different pre-treated groups. RESULTS: After augmenting the mothers' intestinal microbiota through oral BL gavage, the expression of TLR2 and TLR4 in the colon mucosa and colon lymphoid tissues was enhanced and that of HDAC9 in the colon mucosa was decreased, and the proportion of spleen Tregs was increased. The offspring showed similar changes in the AAD model compared with the offspring of the control-group mothers: TLR2 and TLR4 expression in the lungs and the proportion of spleen Tregs increased, HDAC9 expression in the lungs decreased, and AAD-induced airway pathologic characteristics were reversed; additionally, Th1/Th2 and Th9 imbalances were rectified. CONCLUSIONS: This study presents a new framework for the prevention of childhood asthma, elucidating the mechanism of regulating the mother's intestinal microbiome to protect the offspring's early asthma via animal experiments.

2.
Biomed Res Int ; 2020: 6960941, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32626757

RESUMO

The purpose of the present study was to investigate the expressions of hsa-let-7c-5p and TGF-ß signaling-related molecules and their correlations with clinical characteristics in chronic kidney disease (CKD). Twenty-three biopsy specimens of CKD patients and 20 negative control tissues were selected. Quantitative real-time PCR (qPCR) was used for the detection of hsa-let-7c-5p, transforming growth factor ß (TGF-ß) and TGF-ß receptor type 1 (TGF-ßR1) expression levels. Target gene of hsa-let-7c-5p was verified by dual-luciferase reporter assay. A significant decrease of hsa-let-7c-5p expression in CKD tissue was found, compared with that of normal renal tissues (p < 0.01). Expression levels of TGF-ß in CKD were increased, compared with that of normal kidney tissue (p < 0.001). The difference in the expression of TGF-ß R1 between CKD tissues and normal renal tissues was not significant (p > 0.05). A negative correlation was found between the expression of TGF-ß and renal tissue hsa-let-7c-5p levels. Furthermore, hsa-let-7c-5p was identified to regulate TGF- ß1 by directly binding with the 167-173 site in the 3' untranslated region. Decreased hsa-let-7c-5p levels in CKD patients was found to be associated with disease severity, which shows a negative correlation with proteinuria and creatinine levels, and a positive correlation with estimated glomerular filtration rate (eGFR), while relative TGF-ß1 expression had a positive correlation with creatinine level. In summary, changes in hsa-let-7c-5p expression and its target gene TGF-ß are associated with the disease status of CKD. Let-7c-5p may contribute to the pathogenesis of renal fibrosis through TGF-ß signaling, a potential diagnostic and therapeutic target of the disease.

3.
Respir Res ; 21(1): 163, 2020 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-32600344

RESUMO

OBJECTIVE: This study investigated the influence of Coronavirus Disease 2019 (COVID-19) on lung function in early convalescence phase. METHODS: A retrospective study of COVID-19 patients at the Fifth Affiliated Hospital of Sun Yat-sen University were conducted, with serial assessments including lung volumes (TLC), spirometry (FVC, FEV1), lung diffusing capacity for carbon monoxide (DLCO),respiratory muscle strength, 6-min walking distance (6MWD) and high resolution CT being collected at 30 days after discharged. RESULTS: Fifty-seven patients completed the serial assessments. There were 40 non-severe cases and 17 severe cases. Thirty-one patients (54.3%) had abnormal CT findings. Abnormalities were detected in the pulmonary function tests in 43 (75.4%) of the patients. Six (10.5%), 5(8.7%), 25(43.8%) 7(12.3%), and 30 (52.6%) patients had FVC, FEV1, FEV1/FVC ratio, TLC, and DLCO values less than 80% of predicted values, respectively. 28 (49.1%) and 13 (22.8%) patients had PImax and PEmax values less than 80% of the corresponding predicted values. Compared with non-severe cases, severe patients showed higher incidence of DLCO impairment (75.6%vs42.5%, p = 0.019), higher lung total severity score (TSS) and R20, and significantly lower percentage of predicted TLC and 6MWD. No significant correlation between TSS and pulmonary function parameters was found during follow-up visit. CONCLUSION: Impaired diffusing-capacity, lower respiratory muscle strength, and lung imaging abnormalities were detected in more than half of the COVID-19 patients in early convalescence phase. Compared with non-severe cases, severe patients had a higher incidence of DLCO impairment and encountered more TLC decrease and 6MWD decline.


Assuntos
Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/epidemiologia , Pneumonia Viral/diagnóstico , Pneumonia Viral/epidemiologia , Músculos Respiratórios/fisiopatologia , Síndrome Respiratória Aguda Grave/diagnóstico , Adulto , Idoso , Distribuição de Qui-Quadrado , China/epidemiologia , Estudos de Coortes , Convalescença , Teste de Esforço , Feminino , Seguimentos , Volume Expiratório Forçado , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Força Muscular , Pandemias , Alta do Paciente , Radiografia Torácica/métodos , Testes de Função Respiratória , Estudos Retrospectivos , Medição de Risco , Síndrome Respiratória Aguda Grave/epidemiologia , Espirometria/métodos , Centros de Atenção Terciária , Tomografia Computadorizada por Raios X/métodos , Capacidade Vital/fisiologia
4.
Ann Am Thorac Soc ; 2020 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-32692945

RESUMO

RATIONALE: Many clinical studies focused on the epidemiological and clinical characteristics of the inpatients with COVID-19. However, there are few reports about clinical follow-up about discharged patients. OBJECTIVES: This paper aimed to describe the follow-up of patients with COVID-19 in Wenzhou city, Zhejiang, China. METHODS: We retrospectively reviewed 4-week follow-ups in patients with COVID-19, including CT chest scanning, blood test, and oropharyngeal swab test of SARS-CoV-2 RNA. The chest CT scan and blood test were performed on the last day before discharge, two weeks and four weeks after discharge. The oropharyngeal swab test was performed on both one week and two weeks after the discharge. Fifty-one common COVID-19 patients were enrolled in the study. All the CT and clinical data were collected between January, 23 and March, 28, 2020. RESULTS: Compared with the last CT scan before discharge, the abnormalities in lungs were gradually absorbed in the first and second follow-ups after discharge. The cases with focal ground-glass opacity (GGO) reduced from 17.7% to 9.8%. The cases with multiple GGO decreased from 80.4% to 23.5%. The cases with consolidation reduced from 49.0% to 2.0%. The cases with interlobular septal thickening reduced from 80.4% to 35.3%. The cases with subpleural lines reduced from 29.4% to 7.8%. The cases with irregular lines reduced from 41.2% to 15.7%. The lung lesions of 25.5% patients were fully absorbed in the first CT scans after discharge and the rate of lung recovery increased to 64.7% after the second follow-up. Nucleic acid tests turned recurrently positive in 17.6% discharged patients, in which only 33.3% patients complained clinical symptoms. There were no differences in the characteristics of the last CT before discharge between the patients with recurrently positive test and patients with negative test. The lung damages were fully absorbed in 55.6% discharged patients with recurrence of positive SARS-CoV-2 RNA. CONCLUSIONS: The lung damage due to COVID-19 could be reversible for the common COVID-19 patients. A few cases showed recurring positive results of nucleic acid tests after discharge.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32666455

RESUMO

How do Chinese provinces perform in their commitments to carbon emission responsibility? This study extends the carbon emission responsibility allotment proposed by Dietzenbacher et al. (Nat Commun 11:1130, 2020) to a national multi-region setting to analyze provincial, industrial, and bilateral trade emission responsibility allotment in China. This paper uses China's latest multi-region input-output tables in 2012 and 2015, and finds that based on the total amount of ERA carbon emissions, the province with the largest carbon emission responsibility in 2012 is Shandong, followed by Jiangsu and Hebei. From the perspective of industry, the construction is the highest carbon emissions in each province, followed by general and specialist machinery. In the allocation of carbon emissions responsibility in 2015, the credit between Xinjiang and Jiangsu is the largest; Guangdong and Jiangsu have the largest penalty. Among total credits which the province's trade with others, Xinjiang has the highest credit, followed by Shanghai and Jiangsu; among total penalties which the province trade with others, Guangdong Province is the largest penalty province, followed by Guangxi.

6.
Technol Cancer Res Treat ; 19: 1533033820934881, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32552476

RESUMO

BACKGROUND: Whether statins can reduce the incidence of cancers has been an interesting topic in recent years. This meta-analysis aimed to determine the relationship between statin treatment with the risk of hepatocellular carcinoma. METHODS: Studies published up to July 2019 were screened from databases. The data from approved studies were pooled. Random-effects or fixed-effects model was used to calculate the relative risk with 95% CIs in the overall group and subgroups. Sensitivity and meta-regression analyses were performed, and publication bias was evaluated. RESULTS: A total of 18 studies involving 1 611 596 patients were included in this meta-analysis. The overall result showed a significantly reduced risk of hepatocellular carcinoma (relative risk = 0.54, 95% CI: 0.42-0.66) in statin users. In comparison to the risk in nonstatin users, the risk of hepatocellular carcinoma was reduced in all subgroups. The dose of statins and their pharmacokinetics can partly explain the heterogeneity in the overall meta-analysis (I 2 = 94.6%, P = .000). A dose-dependent effect of statin use for the reduced risk of hepatocellular carcinoma was found. CONCLUSIONS: Findings from this meta-analysis support that statin use can significantly reduce the incidence of hepatocellular carcinoma.

7.
Antiviral Res ; 179: 104789, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32353383

RESUMO

Human adenoviruses (HAdVs) often cause mild respiratory infections. These infections, however, can potentially become fatal in immunosuppressive patients. Unfortunately, there has been no specific anti-HAdV drug approved for treatment of HAdV infections. In this study, a time-course transcriptome of HAdV-infected human lung epithelial cells (A549 cells) was performed and compared with perturbation datasets of 890 drug-treated A549 cells from the library of integrated network-based cellular signatures (LINCS) database to predict previously unknown therapeutic drug-HAdV relationships using a characteristic direction (CD) algorithm. We performed experiments to validate a prediction for the anti-diabetic drug rosiglitazone as a candidate drug for treatment of anti-HAdV both in vivo and in vitro. The Type I interferon (IFNs) signaling pathway was negatively regulated during the course of HAdV infection and rosiglitazone increased STAT1 phosphorylation for antiviral IFN response induction. Taken together, this study confirmed the prospect for re-exploitation of this FDA-approved drug as a potential therapeutic for HAdV infections.

8.
Clin Infect Dis ; 2020 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-32392331

RESUMO

BACKGROUND: To illustrate the extent of transmission, identify affecting risk factors and estimate epidemiological modeling parameters of SARS-CoV-2 in household setting. METHODS: We enrolled 35 confirmed index cases and their 148 household contacts, January 2020-February 2020, in Zhuhai, China. All participants were interviewed and asked to complete questionnaires. Household contacts were then prospectively followed active symptom monitoring through the 21-day period and nasopharyngeal and/or oropharyngeal swabs were collected at 3-7 days intervals. Epidemiological, demographic and clinical data (when available) were collected. RESULTS: Assuming that all these secondary cases were infected by their index cases, the second infection rate (SIR) in household context is 32.4% (95% confidence interval [CI] 22.4%-44.4%), with 10.4% of secondary cases being asymptomatic. Multivariate analysis showed that household contacts with underlying medical conditions, a history of direct exposure to Wuhan and its surrounding areas, and shared vehicle with an index patient were associated with higher susceptibility. Household members without protective measures after illness onset of the index patient seem to increase the risk for SARS-CoV-2 infection. The median incubation period and serial interval within household were estimated to be 4.3 days (95% CI; 3.4 to 5.3 days) and 5.1 days (95% CI; 4.3 to 6.2 days), respectively. CONCLUSION: Early isolation of patients with COVID-19 and prioritizing rapid contact investigation, followed by active symptom monitoring and periodic laboratory evaluation, should be initiated immediately after confirming patients to address the underlying determinants driving the continuing pandemic.

9.
J Ethnopharmacol ; 258: 112932, 2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32376368

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Chinese Medicine (TCM) has been widely used as an approach worldwide. Chinese Medicines (CMs) had been used to treat and prevent viral infection pneumonia diseases for thousands of years and had accumulated a large number of clinical experiences and effective prescriptions. AIM OF THE STUDY: This research aimed to systematically excavate the classical prescriptions of Chinese Medicine (CM), which have been used to prevent and treat Pestilence (Wenbing, Wenyi, Shiyi or Yibing) for long history in China, to obtain the potential prescriptions and ingredients to alternatively treat COVID-19. MATERIALS AND METHODS: We developed the screening system based on data mining, molecular docking and network pharmacology. Data mining and association network were used to mine the high-frequency herbs and formulas from ancient prescriptions. Virtual screening for the effective components of high frequency CMs and compatibility Chinese Medicine was explored by a molecular docking approach. Furthermore, network pharmacology method was used to preliminarily uncover the molecule mechanism. RESULTS: 574 prescriptions were obtained from 96,606 classical prescriptions with the key words to treat "Warm diseases (Wenbing)", "Pestilence (Wenyi or Yibing)" or "Epidemic diseases (Shiyi)". Meanwhile, 40 kinds of CMs, 36 CMs-pairs, 6 triple-CMs-groups existed with high frequency among the 574 prescriptions. Additionally, the key targets of SARS-COV-2, namely 3CL hydrolase (Mpro) and angiotensin-converting enzyme 2(ACE2), were used to dock the main ingredients from the 40 kinds by the LigandFitDock method. A total of 66 compounds components with higher frequency were docked with the COVID-19 targets, which were distributed in 26 kinds of CMs, among which Gancao (Glycyrrhizae Radix Et Rhizoma), HuangQin (Scutellariae Radix), Dahuang (Rhei Radix Et Rhizome) and Chaihu (Bupleuri Radix) contain more potential compounds. Network pharmacology results showed that Gancao (Glycyrrhizae Radix Et Rhizoma) and HuangQin (Scutellariae Radix) CMs-pairs could also interact with the targets involving in immune and inflammation diseases. CONCLUSIONS: These results we obtained probably provided potential candidate CMs formulas or active ingredients to overcome COVID-19. Prospectively, animal experiment and rigorous clinic studies are needed to confirm the potential preventive and treat effect of these CMs and compounds.


Assuntos
Betacoronavirus/efeitos dos fármacos , Infecções por Coronavirus/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Tradicional Chinesa , Pneumonia Viral/tratamento farmacológico , Infecções por Coronavirus/virologia , Mineração de Dados , Humanos , Modelos Moleculares , Pandemias , Extratos Vegetais , Pneumonia Viral/virologia , Conformação Proteica , Proteínas Virais
10.
Int Immunopharmacol ; 83: 106453, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32251960

RESUMO

BACKGROUND: Although it is widely accepted that the "hygiene hypothesis" explains the increased incidence of asthma, the lack of suitable animal models hinders further in-depth studies of the underlying molecular immune mechanisms. Therefore, we aimed to develop a robust mouse asthma model to investigate the role of bacteria in preventing asthma. METHODS: BALB/c female mice were fed a mixture of eight common bacterial lysates (BL; Broncho-Vaxom®) and a commercial probiotic (Bifidobacterium tetravaccine tablets) at different concentrations before and during pregnancy to simulate different microbial load levels. Faeces from the mother mice were subjected to bacterial 16S rRNA sequencing to quantify the maternal microbial load. TLR2/4 expression and the proportion of regulatory T cells (Tregs) in the intestinal tract of female mice were determined, and the safety of the microbial load was evaluated. An asthma model was established in the offspring mice after weaning, and the extent of pulmonary pathological changes and Treg proportion were evaluated. RESULTS: A BL concentration of 1 mg/kg enriched the intestinal flora, increased the proportion of Tregs, and increased the expression of TLR2/4 in the maternal mice. The proportion of peripheral blood Tregs was increased, whereas the risk of asthma decreased only in the offspring from mothers with a high microbial load relative to control mice. CONCLUSION: This study established a safe and stable high microbial load maternal-offspring mouse asthma model, laying the foundation for a study on the molecular mechanism underlying the protective effects of a high microbial load against asthma.

11.
Hereditas ; 157(1): 13, 2020 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-32299499

RESUMO

BACKGROUND: Acute mountain sickness has become a heavily researched topic in recent years. However, the genetic mechanism and effects have not been elucidated. Our goal is to construct a gene co-expression network to identify the key modules and hub genes associated with high altitude hypoxia. RESULTS: The GSE46480 dataset of rapidly transported healthy adults with acute mountain sickness was selected and analyzed by weighted gene co-expression network analysis (WGCNA) to construct a co-expression network. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the data set were carried out using Database for Annotation Visualization and Integrated Discovery (DAVID), and the hub genes were selected. We found that the turquoise module was most significantly correlated with acute mountain sickness. The functional enrichment analysis showed that the turquoise module was related to the apoptotic process, protein transport, and translation processes. The metabolic pathway analysis identified hsa03010:ribosome and hsa04144:endocytosis as the most important pathways in the turquoise module. Ten top 10 hub genes (MRPL3, PSMC6, AIMP1, HAT1, DPY30, ATP5L, COX7B, UQCRB, DPM1, and COMMD6) for acute mountain sickness were identified. CONCLUSION: One module and 10 hub genes were identified, which were related to acute mountain sickness. The reference provided by this module may help to elucidate the mechanism of acute mountain sickness. In addition, the hub genes may be used in the future as a biomarker and therapeutic target for accurate diagnosis and treatment.

12.
Nanoscale ; 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32133477

RESUMO

A novel electrocatalytic material VS2@V2C was proposed for the first time and successfully prepared by a one-step hydrothermal method. T-VS2 nanosheets were uniformly and vertically embedded on the V2C (MXene) matrix with a fewer layer structure. Owing to the fast charge transfer process at the interface of the two-phase structure and good conductivity, the composite material showed a lower hydrogen evolution overpotential and a very low Tafel slope in highly alkaline and highly acidic electrolytes (164 mV and 47.6 mV dec-1 in 1.0 M KOH; 138 mV and 37.9 mV dec-1 in 0.5 M H2SO4) under a current density of 20 mV cm-2. More importantly, high-efficiency and stable electrolysis of seawater was achieved at a current density greater than 100 mA cm-2, and the catalytic performance was significantly better than that of platinum-based alloys. First-principles calculations mechanically confirmed that VS2@V2C had higher carrier mobility and lower free energy of hydrogen adsorption. The VS2 nanosheets that grew outwards could provide support to avoid agglomeration on the catalyst surface and the edge sulfur sites of VS2 could promote the binding of adsorbed hydrogen atoms and the desorption of hydrogen molecules. Our work is expected to provide a valuable reference for the design and synthesis of the structure of industrial catalysts for hydrogen production from seawater in the future.

13.
Nanotechnology ; 31(18): 18LT01, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31978905

RESUMO

The electrical breakdown is a bottleneck preventing AgNW networks from being used in high-current electronics such as transparent heaters or similar applications. The process of failure confirms that Joule-heating plays a key role in the formation of cracks perpendicular to the voltage direction. To improve the transfer of Joule heating, solution-processed ZnO nanoparticles were deposited on a gravure printed AgNW random network with good transparency. The AgNW-ZnO nanocomposites show better heating uniformity at higher temperatures because of their improved thermal conductivity. A 57.7% higher power density was obtained without failure, as well as the improved maximum average temperature rise from 72.2 °C to 97.9 °C, after the AgNW was composited with ZnO. This work opens up a new method to study AgNW failures for applications in high-current electronics.

14.
Biomed Pharmacother ; 121: 109564, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31683180

RESUMO

In traditional Chinese medicine (TCM), type 2 diabetes mellitus (T2DM) is regarded as Xiao Ke disease. Liuwei Dihuang pills (LWP), a classical TCM formula, with the function of nourishing kidney yin, has been used for treating Xiao Ke disease in clinic. In this review, we systematically highlighted recent evidence on LWP and T2DM data from clinical and animal studies, summarized the clinical application, pharmacological mechanism and the active compounds of LWP for the treatment of T2DM. This systematic review will provide an insightful understanding of TCM formulas, pharmacological mechanisms, medicinal-disease interactions, and will lay a foundation for the development of new drug therapy for T2DM.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Medicamentos de Ervas Chinesas/administração & dosagem , Hipoglicemiantes/administração & dosagem , Animais , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Humanos , Hipoglicemiantes/farmacologia , Hipoglicemiantes/uso terapêutico , Medicina Tradicional Chinesa
15.
Environ Sci Pollut Res Int ; 27(7): 6796-6812, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31875289

RESUMO

In-depth study of the key sectors and supply chain paths driving coal consumption in China is valuable for effectively formulating coal reduction and replacement policies to achieve sustainable development. This study conducted a structural path analysis, based on the latest publicly available input-output tables and energy use data provided by the World Input-Output Database, to trace China's coal consumption transmission throughout its entire supply chain. The results indicate that investment, exports, and household consumption are important factors in coal consumption. "Electricity, Gas and Water Supply" is a critical sector for, largely indirect, coal consumption. The path of "Electricity, Gas and Water Supply→ intermediate sectors→ Construction→ Investment" accounts for the bulk of coal consumption in China. The node path analysis shows that the 2-node transmission paths, starting from Construction and ending with "Other Non-Metallic Mineral" and "Basic Metals and Fabricated Metal" are important sources of coal consumption induced by investment demand. The 1-node path of "Basic Metals and Fabricated Metal" is an important path for coal consumption induced by export demand. The 1-node path of "Electricity, Gas and Water Supply" is an important path for coal consumption caused by household consumption demand. In order to effectively implement coal reduction and replacement policies, China should control coal consumption in these key sectors and channels and optimize intermediate input and energy use structures.


Assuntos
Carvão Mineral , Abastecimento de Água , China
16.
Int J Mol Sci ; 20(24)2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31842453

RESUMO

BACKGROUND: G-protein-coupled bile acid receptor (TGR5), a membrane bile acid receptor, regulates macrophage reactivity, and attenuates inflammation in different disease models. However, the regulatory effects of TGR5 in ischemia/reperfusion (I/R)-induced kidney injury and inflammation have not yet been extensively studied. Therefore, we hypothesize that Farnesiferol B, a natural TGR5 agonist, could alleviate renal I/R injury by reducing inflammation and macrophage migration through activating TGR5. METHODS: Mice were treated with Farnesiferol B before I/R or sham procedures. Renal function, pathological analysis, and inflammatory mediators were examined. In vitro, the regulatory effects of Farnesiferol B on the Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway in macrophages were investigated. RESULTS: After I/R, Farnesiferol B-treated mice displayed better renal function and less tubular damage. Farnesiferol B reduced renal oxidative stress and inflammation significantly. In vitro, Farnesiferol B treatment alleviated lipopolysaccharide (LPS)-induced macrophage migration and activation, as well as LPS-induced NF-κB activation through TGR5. CONCLUSIONS: Farnesiferol B could protect kidney function from I/R-induced damage by attenuating inflammation though activating TGR5 in macrophages. Farnesiferol B might be a potent TGR5 ligand for the treatment of I/R-induced renal inflammation.


Assuntos
Lesão Renal Aguda/metabolismo , Lesão Renal Aguda/patologia , NF-kappa B/metabolismo , Substâncias Protetoras/farmacologia , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Sesquiterpenos/farmacologia , Transdução de Sinais/efeitos dos fármacos , Lesão Renal Aguda/tratamento farmacológico , Animais , Biópsia , Modelos Animais de Doenças , Feminino , Imuno-Histoquímica , Camundongos , Modelos Biológicos , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Receptores Acoplados a Proteínas-G/metabolismo , Traumatismo por Reperfusão/tratamento farmacológico
17.
J Chem Inf Model ; 59(12): 5065-5073, 2019 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-31765572

RESUMO

Cold-hot nature theory is the core basic theory of traditional Chinese medicine (TCM). "Treating the hot syndrome with cold nature medicine and treating cold syndrome with hot nature medicine" indicates that correct classification of medical properties (cold or hot nature) of Chinese herbal medicines (CHMs) is an important basis for TCM treatment. In this study, we propose a novel multisolvent similarity measure retrieval scheme (MSSMRS) for discriminating CHMs as cold or hot. We explore a multisolvent distance metric learning algorithm to calculate similarity measure of CHM ingredients, and a retrieval scheme for nature identification. First, four solvents (chloroform, distilled water, absolute ethanol, and petroleum ether) are applied to extract ultraviolet (UV) spectrum data of CHM ingredients. Second, we study quantifying the similarity of CHM ingredients to fingerprint similarity. We explore a multisolvent distance metric learning (MSDML) algorithm to measure the similarity of CHM ingredients. MSDML can discover complementary characteristics of different solvent data sets through an optimization algorithm. Finally, a retrieval scheme is designed to analyze the relationship between the CHM ingredients and cold-hot nature. Extensive experimental results demonstrate that CHMs with similar compositions of substances have similar medicinal natures. Experimental evaluations based on the proposed retrieval scheme suggest the effectiveness of MSDML in the identification of the nature of CHMs.

18.
ACS Appl Mater Interfaces ; 11(47): 44282-44292, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31686507

RESUMO

Extensive efforts have been devoted to improving the cycling stability and reversibility of lithium-sulfur batteries. However, unsolved challenges and difficulties still remain in suppressing the shuttle effect, improving the conductivity and structural stability of sulfur cathodes. Here, we report a three-dimensional (3D) grid heterostructure VO2(p) (paramontroseite-VO2) nanorod cluster growing on the surface of two-dimensional V2C (MXene) nanosheets as a high-performance catalytic host for sulfur cathodes. The results of first-principles calculation demonstrate that VO2(p) nanorods can synergize with V2C to enhance the adsorption capacity of host for lithium polysulfides in this host structure and reduce the redox reaction barrier in the conversion of polysulfides to short-chain sulfides. In addition, the high specific surface area and structural stability of the host material can increase the redox reaction kinetics and cyclic reversibility of the electrode. The VO2(p)-V2C/S cathode exhibits outstanding electrochemical performance and excellent reversible discharge capacity (1250 mAh·g-1 at 0.2C), long-term cycling stability (69.1% retention at 2C after 500 cycles), and high sulfur loading cycling capacity (initial areal capacity of 9.3 mAh·cm-2 at 0.2C for 200 cycles). Our research provides a valuable reference for the design of high-performance cathode structures with high sulfur loading.

20.
Mol Med Rep ; 20(4): 3793-3801, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31485626

RESUMO

Lung cancer is one of the leading causes of tumor­associated mortality, and >75% of patients with lung cancer have non­small cell lung cancer (NSCLC). Pemetrexed, a folate antagonist, is a first­line chemotherapy drug for NSCLC that is administered alone or in combination with cisplatin. The present study established in vitro cell models of PTEN inhibition and overexpression, and the effects of the treatment with pemetrexed were investigated in these cell models. Result from the present study demonstrated that treatment with pemetrexed suppressed lung cancer cell proliferation, inhibited mRNA and protein expression levels of anti­apoptotic Bcl2, and increased the mRNA and the protein expression levels of pro­apoptotic p53 and apoptosis regulator BAX. The present study suggested that pemetrexed regulated apoptosis via the inhibition of the mTOR/PI3K/AKT signaling pathway. Additionally, cellular processes associated with the aerobic oxidation of carbohydrates were identified to be significantly inhibited. The present findings suggested that treatment with pemetrexed may exhibit synergistic effects with PTEN on lung cancer cells via the inhibition of the PI3K/AKT/mTOR signaling pathway and through carbohydrate metabolism, and treatment with pemetrexed combined with PTEN overexpression may represent a novel therapeutic strategy for the treatment of NSCLC.


Assuntos
Antineoplásicos/farmacologia , Metabolismo dos Carboidratos/efeitos dos fármacos , Neoplasias Pulmonares/tratamento farmacológico , PTEN Fosfo-Hidrolase/genética , Pemetrexede/farmacologia , Transdução de Sinais/efeitos dos fármacos , Células A549 , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Regulação para Cima/efeitos dos fármacos
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