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1.
J Hazard Mater ; 391: 122218, 2020 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-32044638

RESUMO

This paper conducted catalytic ozonation of CB (chlorobenzene) over a series of MnOx based catalysts with different supports (Al2O3, TiO2, SiO2, CeO2, and ZrO2) at 120 °C. Mn/Al2O3 exhibited highest CB conversion efficiency, ca. 82.92 %, due to its excellent textual properties, O2 desorption, redox ability, and desirable surface adsorbed oxygen species and acidity. O3 conversion all approached nearly 100.0%, with residual <10 ppm. Mn/Al2O3 was further employed to investigate effect of temperature, O3/CB, and space velocity on CB conversion. Hereafter, catalytic ozonation of single and binary VOCs in different types was performed, i.e., CB, DCE (dichloroethane), DCM (dichloromethane), and PhH (Benzene). Conversion results demonstrated aromatics degraded easier than alkanes and more carbon atoms decreased difficulty, as CB∼PhH > DCE∼DCM, and DCE > DCM; but chlorinated substitution increased difficulty, as PhH > CB. Catalytic co-ozonation of CB/DCE indicated that DCE significantly improved CB conversion to reach totally degradation at low O3 input, but inhibited DCE conversion, especially at higher ratio of DCE/CB. Co-ozonation improved ozone utilization efficiency, and maintained the original property of catalyst. By contrast, CB/PhH co-ozonation displayed very mild effects. Finally, critical intermediates during catalytic CB ozonation, i.e., DCM, carboxyl and formic acid, were detected from mass spectrum results.

2.
Curr Neurovasc Res ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32003673

RESUMO

PURPOSE: The current study was conducted in order to investigate the role of Forkhead box O1 and p21-mediated macrophage polarization in postoperative cognitive dysfunction induced by sevoflurane. METHODS: There involved a total of 30 healthy mice that were randomly divided into two groups: control group (without any treatment) and anaesthesia group (treated with sevoflurane inhalation). The effects of sevoflurane on cognitive function (memory) in mice were studied by trace fear conditioned reflex, and the effects of systemic inflammation and behavior after operation were measured by enzyme-linked immunosorbent assay (Elisa), the concentrations of CD163 and tumor necrosis factor -α (TNF- α) were measured. The expression of macrophage phenotype was observed by immunofluorescence staining, the expression levels of M1 and M2 markers mRNA were detected by real-time fluorescence quantitative PCR (RT-PCR), and the expression levels of FoxO1 and p21 were analyzed by immunoblotting (Western blot). RESULTS: Compared with the control group, the freezing time in the anesthesia group was lower than that in the control group (P < 0.01), indicating that sevoflurane anesthesia led to the decrease of cognitive ability. The blood concentrations of CD163 and TNF- α increased significantly at 24 hours after operation with sevoflurane anesthesia (P < 0.05). Fluorescence microscopic observation showed that M2 was the main type of macrophages in normal tissues, while M1 and M2 phenotypes were highly expressed in sevoflurane anesthetized tissues at the same time, especially in M1 phenotypes (P < 0.01). The polarization of macrophages in the anesthetic group showed the high level of M1 mRNA, and the expression levels of TNF-a, monocyte chemotactic protein 1(MCP-1) and Interleukin-6 (IL-6)mRNA in the anesthetic group were significantly higher than those in the control group (P < 0.05). The expression levels of M2 mRNA such as transforming growth factor-ß (TGF- ß) and IL-10 were significantly lower than those in the control group (P < 0.05). Compared with the control group, the expression of FoxO1 and p21 protein in the anesthesia group was significantly lower than that in the control group with significant statistical difference (P < 0.01). CONCLUSION: This study offers theoretical basis and insight for further understanding the prevention and treatment of cognitive dysfunction induced by anesthetic drugs.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31988071

RESUMO

BACKGROUND: Genome-wide association studies (GWASs) have identified dozens of loci associated with colon and rectal adenocarcinoma risk. As tissue-specific super-enhancers (SEs) play important roles in tumorigenesis, we systematically investigate SEs and inner variants in established GWAS loci to decipher the underlying biological mechanisms. METHODS: Through a comprehensive bioinformatics analysis on multi-omics data, we screen potential single nucleotide polymorphisms (SNPs) in cancer-specific SEs, and then subject them to a two-stage case-control study totally containing 4929 cases and 7083 controls of Chinese population. A series of functional assays including reporter gene assays, electrophoretic mobility shift assays (EMSAs), CRISPR-cas9 genome editing, chromosome conformation capture (3C) assays and cell proliferation experiments are performed to characterize the variant's molecular consequence and target genes. RESULTS: The SNP rs11064124 in 12p13.31 is found significantly associated with the risk of colon and rectal adenocarcinoma with an odds ratio (OR) being 0.87 (95% confidence intervals (CI) = 0.82-0.92, P = 8.67E-06). The protective rs11064124-G weakens the binding affinity with vitamin D receptor (VDR), and increases the enhancer's activity and interactions with two target genes' promoters, thus co-activates the transcription of CD9 and PLEKHG6 which are both putative tumor suppressor genes for colon and rectal adenocarcinoma. CONCLUSIONS: Our integrative study highlights a SE polymorphism rs11064124, and two susceptibility genes CD9 and PLEKHG6 in 12p13.31 for colon and rectal adenocarcinoma. IMPACT: These findings suggest a novel insight for genetic pathogenesis of colon and rectal adenocarcinoma, involving transcriptional co-activation of diverse susceptibility genes via the SE element as a gene regulation hub.

4.
Nanotechnology ; 31(17): 175401, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-31899895

RESUMO

The increasing demand in energy consumption and the use of clean energy from sustainable energy sources have driven the research in the development of advanced materials for Li-ion and Na-ion batteries. In this work, we have developed a simple technique to synthesize a porous Sb structure through a galvanic replacement reaction between Sb3+ and Zn particles. The porous Sb structure consists of a three-dimensional-hierarchical structure with tree-like nanoscale Sb dendrites. The Sb in the nanodendrites is crystal of a rhombohedral structure. We construct Li-/Na-ion half cells and Li-/Na-ion full cells with the Sb nanodendrites as the active material in the working electrode and anode, respectively, and introduce an additive of vinylene carbonate for the Li-ion half/full cells and an additive of fluoroethylene carbonate for the Na-ion half/full cells. All the Li-/Na-ion half cells and Li-/Na-ion full cells exhibit excellent electrochemical performance and cycling stability. Such excellent performance can be attributed to the synergistic interaction between the three-dimensional-dendritic structure and electrolyte, which likely ensures fast transport of ions and electrons and the formation of a stable solid-state interphase.

5.
Langmuir ; 36(1): 20-27, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31825231

RESUMO

In the electrochemical Bunsen reaction, which is the key reaction of the sulfur-iodide cycle, the main overpotential corresponds to the oxidation of SO2. The catalysts currently used for the liquid phase electrocatalytic oxidation of SO2 are mainly based on noble metals, which have excellent corrosion resistance and catalytic activity in an acidic environment. To improve the performance of the commercial Pt catalyst, a Pt catalyst with reduced graphene oxide (RGO) and carbon black as a hybrid support was synthesized by the microwave-assisted polyol reduction process. The large two-dimensional planar structure of RGO was a better anchor for Pt nanoparticles, and a catalyst with fine and uniform distribution of Pt particles was obtained. The carbon black interior prevented the agglomeration of RGO, forming a stereoscopic catalyst structure. The electrochemical test results showed that the RGO/carbon black hybrid support catalyst possessed a higher electrocatalytic activity than the single support catalysts and could be used as an efficient and stable catalyst for the SO2 electrolyzer.

6.
Nanotechnology ; 31(13): 135404, 2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-31810067

RESUMO

There is a great need to develop sustainable and clean energy storage devices and systems of high-energy and high-capacity densities. In this work, we synthesize antimony (Sb) nanocrystal-anchored hollow carbon microspheres (Sb@HCMs) via the calcination of cultivated yeast cells and the reduction of SbCl3 in an ethylene glycol solution on the surface of hollow carbon microspheres. The Sb@HCMs possess hollow and porous structure, and the Sb is present in the form of nanocrystals. Using the Sb@HCMs as the active-electrode material, we assemble lithium (Li)-ion half cells and full cells and investigate their electrochemical performance. The Li-ion half cells possess a charge capacity of 605 mA h g-1 after 100 cycles at a current density of 100 mA g-1 and a charge capacity of 469.9 mA h g-1 at a current density up to 1600 mA g-1, which is much higher than the theoretical capacity of 372 mA h g-1 for commercial graphite electrode. The Li-ion full cells with Sb@HCMs//LiCoO2 deliver a charge capacity of 300 mA h g-1 at a current density of 0.2 A g-1 after 50 cycles, and have potential in applications of energy storage.

7.
Asian J Surg ; 43(2): 423-427, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31350090

RESUMO

OBJECTIVE: To report our experience in treating endophytic renal tumor by robot-assisted partial nephrectomy (RPN) with a standard laparoscopic ultrasound probe and our original approach for separating renal mass. METHODS: All patients with endophytic renal tumor who underwent RPN in our department from January 2015 to December 2017 were retrospectively analyzed. All surgeries were performed by transperitoneally by a single, experienced surgeon. Baseline characteristics, tumor characteristics, operative profile and follow-up data were collected. RESULTS: Among these 29 patients, 23 patients were male. The mean age of patients was 47.42 years old. 69% (20/29) tumors were completely endophytic tumors. The average tumor size was 3.1 cm. The average R.E.N.A.L. score was 9.0. Tumors from 3 patients were pathologically confirmed to be renal angiomyolipomas. And the rest were malignant including 23 clear cell renal cell carcinoma and 3 chromophobe renal cell carcinoma. The mean operative time was 3.0 h. The mean warm ischemia time was 22.3 min. All tumor margins were pathologically confirmed negative. No patient needed blood transfusion. Two patients presented had Grade II complication. Both of them had a fever after surgery with body temperature over 38 °C, leading to a prolonged postoperative hospitalization time. The mean postoperative hospitalization time was 6.8 days. During mean 21.3-month follow-up, no patient was confirmed to relapse by CT scan. CONCLUSION: RPN with a standard laparoscopic ultrasound probe is safe, effective and feasible in treating endophytic renal tumor. Our original approach for separating renal mass can avoid some problems caused by the standard laparoscopic ultrasound probe.

8.
Life Sci ; 241: 116826, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31479678

RESUMO

AIMS: Hepatitis is a kind of liver dysfunction and usually refers to a variety of pathogenic factors. Circular RNA (circRNA) participates in diverse diseases. This study aimed to explore the roles and regulatory mechanisms of the circRNA-4099 in L02 cell line. MAIN METHODS: Cells were induced with H2O2 dilutions and transfected with circRNA-4099 overproduction vectoer and the specific siRNA (si-circRNA-4099), as well as microRNA-706 (miR-706) mimic or the corresponding controls. Cell viability was detected with the CCK-8. The apoptotic rate was determined by the annexin v-FITC/PI with flow cytometer. The expression of circRNA-4099 or miR-706 was quantified depending on qRT-PCR. The reactive oxygen species (ROS) level was examined through ROS assay. The relative proteins were individually determined via western blot. The relationship between the circRNA-4099 and miR-706 was detected through bioinformatics analysis and luciferase reporter assay. KEY FINDINGS: H2O2 induced inhibition of viability and promotion of apoptosis, ROS production and cell fibrosis as well as keap1/Nrf2 and p38MAPK cascades on L02 cell line. circRNA-4099 was stimulated by H2O2. Plentiful circRNA-4099 augmented H2O2-resulted damage by inhibiting miR-706. miR-706 mimic partly abolished the influence of circRNA-4099 on L02 cells. Meanwhile, circRNA-4099 silence exerted opposite effect on these elements above. SIGNIFICANCE: circRNA-4099 aggravated H2O2-induced injury by inhibiting miR-706 through triggering keap1/Nrf2 and p38MAPK in the L02 cells.

9.
Onco Targets Ther ; 12: 10361-10374, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819519

RESUMO

Purpose: Perineural invasion (PNI) is the neoplastic invasion of nerves by cancer cells, a process that may prove to be another metastatic route besides direct invasion, lymphatic spread, and vascular dissemination. Given the increasing incidence and association with poor prognosis, revealing the pathogenesis of perineural invasion is of great importance. Materials and methods: Four datasets related to PNI were downloaded from the Gene Expression Omnibus database and used to construct weighted gene co-expression network analysis (WGCNA). The intersection of potential pathways obtained from further correlation and enrichment analyses of different datasets was validated by the coculture model of Schwann cells (SCs), flow cytometry and immunohistochemistry (IHC). Results: GSE7055 and GSE86544 datasets were brought into the analysis for there were some significant modules related to PNI, while GSE103479 and GSE102238 datasets were excluded for insignificant differences. In total, 13,841 genes from GSE86544 and 10,809 genes from GSE7055 were used for WGCNA. As a consequence, 19 and 26 modules were generated, respectively. The purple module of GSE86544 and the dark gray module of GSE7055 were positively correlated with perineural invasion. Further correlation and enrichment analyses of genes from the two modules suggested that these genes were mainly enriched in cell cycle processes; especially, the terms S/G2/M phase were enriched. Three kinds of cells grew vigorously after coculture with SCs ex vivo. The Ki67 staining of the cervical cancer samples revealed that the Ki67 index of cancer cells surrounding nerves was higher than of those distant ones. Conclusion: Our work has identified cancer cell proliferation as a common response to neural cancerous microenvironments, proving a foundation for cancer cell colonization and metastasis.

10.
Immunol Res ; 67(4-5): 373-381, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31773491

RESUMO

The aim of this study was to evaluate the changes in the three subsets of monocyte (classical, intermediate, and non-classical) and the expression of human leukocyte antigen-DR (HLA-DR) on monocyte subsets during MP pneumonia in children. Monocyte subsets were analyzed in the peripheral blood of healthy volunteers and MP pneumonia patients at the stages of admission and remission after clinical therapy. They were defined as classical (CD14+CD16-), intermediate (CD14brightCD16+), and non-classical (CD14dimCD16+) using flow cytometry. Furthermore, three subsets of monocyte were analyzed for the expression of HLA-DR. Patients with MP pneumonia at admission had a higher proportion of intermediate and non-classical monocytes than healthy subjects (all P < 0.05). The proportion of intermediate subset and non-classical subset was lower in MP pneumonia patients at remission than at admission (all P < 0.05). In comparison with the other monocyte subsets, intermediate subset showed a significantly higher percentage of HLA-DR in MP pneumonia patients at admission (P < 0.05). Further analysis revealed that the expression of HLA-DR on intermediate subset was lower in severe patients than in non-severe patients (P < 0.05).Our data has shown for the first time that MP pneumonia is associated with the increased proportion of non-classical and intermediate monocytes, indicating the involvement of monocyte-related mechanisms in the pathogenesis of this disease. Additionally, the decreased expression of HLA-DR on CD14brightCD16+ subset may be a potential indicator of the severity of MP pneumonia.

11.
IEEE Trans Biomed Circuits Syst ; 13(6): 1563-1574, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31751286

RESUMO

This paper proposed a wearable smart sEMG recorder integrated gradient boosting decision tree (GBDT) based hand gesture recognition. A hydrogel-silica gel based flexible surface electrode band is used as the tissue interface. The sEMG signal is collected using a neural signal acquisition analog front end (AFE) chip. A quantitative analysis method is proposed to balance the algorithm complexity and recognition accuracy. A parallel GBDT implementation is proposed featuring a low latency. The proposed GBDT based neural signal processing unit (NSPU) is implemented on an FPGA near the AFE. A RF module is used for wireless communication. A hand gesture set including 12 gestures is designed for human-computer interaction. Experimental results show an overall hand gesture recognition accuracy of 91%.

12.
Nanomaterials (Basel) ; 9(11)2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31752211

RESUMO

P25 loaded few layered molybdenum disulfide (MoS2) nanosheets (P25@MoS2) are successfully synthesized through a facile one-step hydrothermal process. The bi-catalytic activities, i.e., photocatalytic and electrocatalytic activities, of the as-prepared nanomaterials have been investigated. For the as-prepared products, the photocatalytic performances were investigated by degrading simulated pollutant under sunlight irradiation, and the hydrogen evolution reaction evaluated the electrocatalytic performances. The results indicate that P25@MoS2 possesses excellent activities in both photocatalysis and electrocatalysis. The presence of MoS2 broadens the light absorption range of P25 and improves the separation and transformation efficiency of photogenerated carriers, thus improving its photocatalytic performance. The existence of P25 inhibits the aggregation of MoS2 to form more dispersed MoS2 nanosheets with only few layers increasing its active sites. Thereby, the electrocatalytic performance is heightened. The excellent multifunction makes the as-prepared P25@MoS2 a promising material in the fields of environment and energy.

13.
J Biochem Mol Toxicol ; 33(12): e22374, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31702096

RESUMO

The main purpose of the current study is to reveal the anticancer action of limonin against benzo(a)pyrene [B(a)P]-treated lung carcinogenesis in Swiss albino mice and A549 lung cancer cells. B(a)P was orally supplemented (50 mg/kg body weight) twice a week for four weeks induction of lung cancer in mice. The lung weight, body weight, incidence of tumor, lipid peroxidation, carcinoembryonic antigen (CEA), enzymatic and nonenzymatic antioxidants (superoxide dismutase, GPx, glutathione, glutathione reductase, catalase, and glutathione S-transferase), serum marker enzymes (aryl hydroxylase, lactate dehydrogenase, 5'-nucleotidases, and γ-glutamyl transpeptidase), and inflammatory mediators (interleukin-1ß, interleukin-6, and tumor necrosis factor-α) were estimated. Moreover, a histopathological study of lung tissues was supported by the biochemical analysis. Furthermore, the anticancer activity of limonin on A549 cells was measured by cell viability, production of reactive oxygen species (ROS), apoptotic morphological changes by AO/EtBr staining. Additionally, the status of apoptosis protein (caspase-9 and -3) expressions was analyzed by the colorimetric analysis. B(a)P-induced mice showed increased lipid peroxidation, CEA, serum marker enzymes and inflammatory cytokines levels with simultaneously decreased in the nonenzymatic and enzymatic antioxidants levels. Limonin supplements significantly reverted back to all these changes in this manner, showing the efficiency of anticancer effect. Furthermore, our in vitro study also supported the anticancer effect of the treatment of limonin-enhanced apoptosis by loss of cell viability, improved ROS production, apoptotic morphological changes, and apoptosis protein expression were analyzed. Overall, these results suggest the anticancer potential of limonin against B(a)P-induced lung cancer in Swiss albino mice and A549 lung cancer cells.


Assuntos
Anticarcinógenos/uso terapêutico , Antioxidantes/uso terapêutico , Apoptose/efeitos dos fármacos , Benzo(a)pireno/farmacologia , Carcinogênese/induzido quimicamente , Carcinogênese/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Limoninas/uso terapêutico , Neoplasias Pulmonares/prevenção & controle , Células A549 , Animais , Benzo(a)pireno/administração & dosagem , Antígeno Carcinoembrionário/metabolismo , Caspase 3/metabolismo , Caspase 9/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Citocinas/metabolismo , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Neoplasias Pulmonares/patologia , Camundongos , Camundongos Endogâmicos C57BL , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Carga Tumoral
14.
Cartilage ; : 1947603519888787, 2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31735077

RESUMO

OBJECTIVE: Long non-coding RNA 01534 (LINC01534) is highly expressed in the tissues of patients with osteoarthritis (OA). This study investigated the mechanism of LINC01534 on abnormal metabolic dysfunction in OA chondrocytes induced by interleukin-1ß (IL-1ß). METHODS: The quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expressions of LINC01534, aggrecan, collagen II, and matrix metalloproteinase (MMPs) in OA cartilage tissue or OA chondrocyte model induced by IL-1ß. The expressions of aggrecan and collagen II in the chondrocyte were detected by Western blot. The levels of tumor necrosis factor-α (TNF-α), IL-8, IL-6, MMP-13, MMP-9, MMP-3, and prostaglandin E2 (PGE2) in chondrocyte were determined by enzyme-linked immunosorbernt assay. Bioinformatics, dual luciferin gene reporting, RNA pulldown, and Northern blot were used to determine the interaction between LINC01534 and miR-140-5p. RESULTS: The results showed that LINC01534 was upregulated in both OA cartilage tissue and OA chondrocyte model. In addition, silencing LINC01534 significantly alleviated the inhibitory effect of IL-1ß on expressions of aggrecan and collagen II in chondrocytes, and significantly downregulated the expression of matrix metalloproteinases in IL-1ß-induced chondrocytes. Meanwhile, silencing LINC01534 also significantly inhibited the productions of proinflammatory factors NO, PGE2, TNF-α, IL-6, and IL-8 in the IL-1ß-induced chondrocytes. Furthermore, miR-140-5p was confirmed to be a direct target of LINC01534. More importantly, inhibition of miR-140-5p significantly reversed the inhibitory effect of silencing LINC01534 on abnormal matrix degradation in the IL-1ß-induced chondrocyte model of OA. CONCLUSION: Therefore, LINC01534 could promote the abnormal matrix degradation and inflammatory response of OA chondrocytes through the targeted binding of miR-140-5p.

15.
Sci Rep ; 9(1): 16906, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729438

RESUMO

Erdite is a rare sulphide mineral found in mafic and alkaline rocks. Only weakly crystallised fibrous erdite has been artificially synthesised via evaporation or the hydrothermal method, and the process generally requires 1-3 days and large amounts of energy to complete. In this study, well-crystallised erdite nanorods were produced within 3 h by using MnO2 as an auxiliary reagent in a one-step hydrothermal method. Results showed that erdite could synthesised in nanorod form with a diameter of approximately 200 nm and lengths of 0.5-3 µm by adding MnO2; moreover, the crystals grew with increasing MnO2 addition. Without MnO2, erdite particles were generated in irregular form. The capacity of the erdite nanorods for tetracycline (TC) adsorption was 2613.3 mg/g, which is higher than those of irregular erdite and other reported adsorbents. The major adsorption mechanism of the crystals involves a coordinating reaction between the -NH2 group of TC and the hydroxyl group of Fe oxyhydroxide produced from erdite hydrolysis. To the best of our knowledge, this study is the first to synthesise erdite nanorods and use them in TC adsorption. Erdite nanorods may be developed as a new material in the treatment of TC-containing wastewater.

16.
IEEE Trans Biomed Circuits Syst ; 13(6): 1383-1392, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31613777

RESUMO

As the average age of the population goes higher, the people undergo hip arthroplasty surgery to replace their stiff and painful damaged hip joints. To reduce the risk factor after the surgery we developed a visual aided system in previous works. In order to solve the integration problems of commercial camera with other sensors into the femoral head and to minimize the area and power consumption, in this paper we propose a CMOS image sensor of resolution 200 × 200 specially designed for the application in which each individual pixel measures around 15 µm × 15 µm in size and the image sensor chip size measures about 3.5 mm × 3.5 mm. The proposed sensor is simulated with the input current variations from 2 pA to 100 pA for the individual pixels and the corresponding measurements for each pixel range from 2 mV to 855 mV. Besides, we put forward a new method of pattern detection and recognition in the blood-covered situation, which provides an accurate segmentation of patterns from the blood. All the detected patterns are recognized by generating its right 9-bit binary ID required for the pose estimation calculation. Furthermore, to reduce system power consumption, we implement algorithms on FPGA to process the image data pixel by pixel and transmit it directly to the computer for post-processing. Experimental results show that the pattern detection rate goes as high as 99%, which is 5% better in accuracy compared to the top hat algorithm. The power consumption of the system is 213 mW, which is a 70% decrease compared to our previous work.

17.
J Biomech ; 97: 109415, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31630776

RESUMO

Intraoperative measurement of hip posture is the basis for assessing hip range of motion (ROM) and predicting postoperative functional limits allowable for activities of daily living. Although computer navigation for total hip arthroplasty (THA) has improved the accuracy of intraoperative ROM evaluation, it has not gained widespread popularity due to its complex and time-consuming protocol. We therefore developed an inertial measurement unit-based hip smart trial system (IMUHST) for intraoperative monitoring of hip posture. An in vitro validation experiment was conducted using bone models with a three-dimensional measurement model as the reference standard. The absolute mean error, Bland - Altman analysis and intra-class correlation coefficient demonstrated that the validity and reliability of this system meets the requirement for clinical application. Given that monitoring posture is the basis for evaluating the direction(s) of potential impingement, subluxation and dislocation, the IMUHST is a promising development direction of computer assisted surgery in THA.

18.
Analyst ; 144(22): 6641-6646, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31595888

RESUMO

The detection of the HPV L1 protein provides information about the infection status of the virus, reflects the replication status of the HPV virus in cervical cells, and helps understand the regression and progress of cervical lesions. Herein, we report a novel laser desorption ionization mass spectrometry (LDI MS) method for the sensitive detection of the HPV 16 L1 protein, based on non-covalent competitive adsorption between the HPV 16 L1 aptamer and melamine on gold nanoparticles (AuNPs). The intensity of the MS signal corresponding to the mass tag shows a linear relationship with the HPV 16 L1 concentration in the range 2-80 ng mL-1, with a limit of detection (LOD) of 58.8 pg mL-1. Using this method, the HPV 16 L1 protein is quantitatively analyzed in both clinical and vaccine samples. The described method is simple and has high sensitivity and good reliability.


Assuntos
Proteínas do Capsídeo/análise , Nanopartículas Metálicas/química , Proteínas Oncogênicas Virais/análise , Adsorção , Aptâmeros de Nucleotídeos/química , Sequência de Bases , Proteínas do Capsídeo/química , Ouro/química , Papillomavirus Humano 16/química , Limite de Detecção , Espectrometria de Massas/métodos , Proteínas Oncogênicas Virais/química , Vacinas contra Papillomavirus/análise , RNA/química , Triazinas/química
19.
Anal Chem ; 91(21): 13582-13590, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31603309

RESUMO

Acetohydroxyacid synthase (AHAS) exists in plants and many microorganisms (including gut flora) but not in mammals, making it an attractive drug target. Fluorescent-based methods should be practical for high-throughput screening of inhibitors. Herein, we describe the development of the first AHAS fluorogenic assay based on an intramolecular charge transfer (ICT)-based fluorescent probe. The assay is facile, sensitive, and continuous and can be applied toward various AHASs from different species, AHAS mutants, and crude cell lysates. The fluorogenic assay was successfully applied for (1) high-throughput screening of commerical herbicides toward different AHASs for choosing matching herbicides, (2) identification of a Soybean AHAS gene with broad-spectrum herbicide resistance, and (3) identification of selective inhibitors toward intestinal-bacterial AHASs. Among the AHAS inhibitors, an active agent was found for selective inhibition of obesity-associated Ruminococcus torques growth, implying the possibility of AHAS inhibitors for the ultimate goal toward antiobesity therapeutics. The fluorogenic assay opens the door for high-throughput programs in AHAS-related fields, and the design principle might be applied for development of fluorogenic assays of other synthases.

20.
Medicine (Baltimore) ; 98(42): e17327, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31626088

RESUMO

To explore the association of a disintegrin and metalloprotease 33 (ADAM33) polymorphisms with childhood asthma susceptibility, we conducted this case-control study.In this case-control study, we selected 96 asthma children and 86 healthy children to conduct the genotyping of ADAM33 polymorphisms through polymerase chain reaction-direct sequencing (PCR-DS). Hardy-Weinberg equilibrium (HWE) status in the control group was detected adopting chi-square test. Frequency differences of genotypes, alleles, and haplotypes were compared by chi-square test between the case and control groups. Linkage disequilibrium (LD) between polymorphisms was checked using Haploview software. Association intensity of the polymorphisms with the disease risk was assessed by odds ratio (OR) and 95% confidence interval (95%CI).The frequency of rs678881 GA genotype was obviously higher in cases than in controls (P = .03) and the carriage of this genotype conferred higher risk of asthma among children than GG genotype (OR = 2.03, 95%CI = 1.05-3.91). However, neither rs2280089 nor rs2853209 polymorphism was significantly associated with the risk of childhood asthma. Strong LD was found among rs678881, rs2280089 and rs2853209, and haplotype GGT was distinctly associated with the risk of asthma in children (OR = 0.28, 95%CI = 0.13-0.57).ADAM33 rs678881 polymorphism is significantly correlated with increased susceptibility to asthma in Chinese Han children. Besides, haplotype GGT among the 3 polymorphisms was obviously associated with decreased risk of childhood asthma.


Assuntos
Proteínas ADAM/genética , Asma/genética , Alelos , Asma/sangue , Asma/etnologia , Estudos de Casos e Controles , Criança , Pré-Escolar , China/epidemiologia , Feminino , Predisposição Genética para Doença/etnologia , Genótipo , Haplótipos , Humanos , Masculino , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único , Fatores de Risco
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