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1.
J Nanosci Nanotechnol ; 20(2): 789-801, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383074

RESUMO

Magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites were prepared via a solution combustion process, and were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), Energy dispersive X-ray Spectroscopy (EDX) and vibrating sample magnetometer (VSM). The magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites were employed to remove Congo red (CR) from aqueous solution, and the adsorption process was optimized by response surface methodology (RSM). The optimum conditions were the silica content of 12.6 wt%, the calcination temperature of 501 °C and the pH value of 7.13. The adsorption kinetics and the adsorption isotherm of CR onto magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites at room temperature were investigated, and the intraparticle diffusion kinetics model and Redlich-Peterson isotherm model fitted well the respective process.

2.
J Nanosci Nanotechnol ; 20(2): 957-964, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383092

RESUMO

Magnetic Ni0.7Co0.3Fe2O4 nanoparticles were prepared via the alcohol-solution combustion process, the morphology and magnetic property of them were investigated by the scanning electron microscopy (SEM), the transmission electron microscope (TEM), the X-ray diffraction (XRD), the vibrating sample magnetometer (VSM), the energy dispersive spectroscopy (EDS) and the Brunauer-Emmett-Teller (BET). The as-prepared magnetic Ni0.7Co0.3Fe2O4 nanoparticles calcined at 400 °C for 2 h with absolute alcohol of 30 mL were characterized with the average nanoparticle size of around 30 nm and the specific magnetization (Ms) of 14.7 emu/g. The adsorption characteristics of reactive blue (RB) from aqueous solutions onto Ni0.7Co0.3Fe2O4 nanoparticles were investigated by UV spectroscopy at room temperature; the adsorption kinetics data were in good agreement with the pseudo-second-order kinetic model in the initial RB concentrations of 100-400 mg/L. Compared with Langmuir and Freundlich models, the Temkin model (correlation coefficient R² =0.9998) could be used to evaluate the adsorption isotherm of RB onto the magnetic Ni0.7Co0.3Fe2O4 nanoparticles at room temperature, which revealed that the adsorption of RB onto the magnetic Ni0.7Co0.3Fe2O4 nanoparticles was a hybrid of monolayer and multilayer adsorbing mechanism.

3.
J Microbiol ; 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31463790

RESUMO

rRNA gene high-throughput sequencing was performed in the conjunctival swab samples to investigate the composition of the OS bacterial community in DE (n=35) and NDE (n=54) and compared the composition of MGD (n=25) and NMGD (n=10) among DE subjects. Deep sequencing of OS 16S rDNA from DE (n=35) and NDE (n=54) demonstrated great a difference in alpha and beta diversity between the OS bacterial flora (P < 0.05). The similar OS microbial structures were shown at the phylum and genus levels by bioinformatics analysis between them, and in LEfSe (linear discriminant analysis effect size) analysis, Bacteroidia and Bacteroidetes were enriched in DE, while Pseudomonas was plentiful in NDE (linear discriminant analysis [LDA] > 4.0). Among the DE group, there was no significant difference in α and ß diversity between MGD and NMGD (P > 0.05). Surprisingly, Bacilli was the dominant microbe in MGD, and Bacteroidetes was the superior bacteria in NMGD among DE subjects (LDA > 4.0). Different diversity of OS bacteria composition between DE and NDE and the altered diversity of OS bacteria may play an important role in DE. Moreover, the lower dominance of OS bacteria in DE may be associated with the occurrence and development of DE. Although there was no significant difference in alpha and beta analysis, the OS dominant microbe between MGD and NMGD among DE was different.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31271432

RESUMO

Solitary fibrous tumor/hemangiopericytomas (SFT/HPCs) are mesenchymal tumors characterized by "staghorn" blood vessels and collagen deposition. Little is known about SFT/HPCs with papillary architecture. We summarized the clinicopathologic features of 12 patients with papillary SFT/HPCs (8 males and 4 females; median age: 59 years), including 8 previously reported cases. Tumors were present in the meninges (75%, 9/12), adrenal gland (8%, 1/12), orbit (8%, 1/12), or spinal canal (8%, 1/12). Six tumors (50%) had a true papillary architecture with fibrovascular cores and 6 tumors (50%) had a pseudopapillary architecture with vascular cores. Nuclear staining for STAT6 was present in all tested tumors (10/10). RT-PCR indicated NAB2 ex6-STAT6 ex17 fusion in 4 tumors (80%, 4/5) and NAB2 ex4-STAT6 ex2 fusion in 1 tumor (20%, 1/5). Five patients (42%, 5/12), all with tumors in the meninges, developed local recurrence at a median of 61 months after surgery (range: 56-165 months; mean: 88.6 months). These results indicated that the papillary architecture is a morphological form of SFT/HPCs. The recognition of this pattern, with appropriate immunohistochemical analysis and assessment of NAB2-STAT6 fusion, should facilitate the distinction of these rare neoplasms from morphologically similar tumors in the meninges, lung, pleura, and soft tissue.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31309678

RESUMO

Enormous efforts have been devoted to carbon dioxide (CO2) reduction by utilizing various driving forces, such as heat, electricity and radiation. However, efficient reduction of CO2 is still challenging because of the sluggish kinetics. Recent pioneering works from several groups including us have demonstrated that coupling of solar energy and thermal energy serves as a novel and promising strategy to promote activity and/or manipulate selectivity in CO2 reduction. Herein, we will clearly clarify the definition and principles of coupling of solar energy and thermal energy, and comprehensively review the status and prospects of CO2 reduction by coupling of solar energy and thermal energy. Catalyst design, reactor configuration, photo-mediated activity/selectivity and mechanism studies in photo-thermo CO2 reduction will be emphasized. This review aims to promote the understandings towards CO2 activation and provide guidelines for the design of new catalysts for efficient CO2 reduction.

6.
J Nanosci Nanotechnol ; 19(12): 8180-8186, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31196342

RESUMO

A novel solution combustion and gel calcination process for the preparation of ZnO nanoparticles and ZnO/Ni0.5Zn0.5Fe2O4 nanocomposites was introduced. The phase identification, morphologies and magnetic properties of ZnO nanoparticles and ZnO/Ni0.5Zn0.5Fe2O4 nanocomposites were confirmed by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscope (TEM), vibrating sample magnetometer (VSM). The results showed that the concentration of zinc nitrate and the calcination temperature were two key factors for the grain size, crystallinity and properties of ZnO nanoparticles, while, the mass ratio of ZnO and Ni0.5Zn0.5Fe2O4 in ZnO/Ni0.5Zn0.5Fe2O4 nanocomposites was the important factor for the specific magnetizations (Ms) of ZnO/Ni0.5Zn0.5Fe2O4 nanocomposites.

7.
BJU Int ; 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31166645

RESUMO

OBJECTIVES: To evaluate, using a rat model of non-bacterial prostatic inflammation, the prostaglandin production and expression profiles of E-series prostaglandin (EP) receptor subtypes, which are reportedly implicated in the development of overactive bladder, in the bladder mucosa, and to investigate the effect of EP receptor type 4 (EP4) blockade on bladder overactivity after prostatic inflammation. METHODS: Male Sprague-Dawley rats were used. Prostatic inflammation was induced by formalin injection (5%; 50 µL per lobe) into the bilateral ventral lobes of the prostate. At 10 days after induction of prostatic inflammation or vehicle injection, bladder tissues from the deeply anaesthetized rats were harvested and separated into mucosal and detrusor layers. Then, prostaglandin E2 (PGE2) concentrations and protein levels of PGE2 receptors (EP1-4) in the bladder mucosa and detrusor were measured by ELISA and Western blotting, respectively. In separate groups of control and formalin-treated rats, awake cystometry was performed to evaluate the changes in bladder activity after prostatic inflammation. In addition, the effect of intravesical administration of a selective EP4 antagonist (ONO-AE3-208; 30 µm) on bladder activity was evaluated in control rats and rats with prostatic inflammation. RESULTS: PGE2 concentration and protein levels of EP4, but not other EP receptor subtypes, in the bladder mucosa and detrusor layers were significantly increased in formalin-injected rats vs vehicle-injected control rats. In cystometry, rats with prostatic inflammation exhibited a significant decrease in intercontraction intervals (ICIs) compared with control rats. Intravesical application of ONO-AE3-208 (30 µm), but not vehicle application, significantly increased ICIs in rats with prostatic inflammation, whereas ONO-AE3-208 at this concentration did not significantly affect any cystometric values in control rats. CONCLUSIONS: Because intravesical administration of an EP4 antagonist effectively improved bladder overactivity after prostatic inflammation, EP4 activation, along with increased PGE2 production in the bladder mucosa, seems to be an important contributing factor to bladder overactivity induced by prostatic inflammation. Thus, blockade of EP4 in the bladder could be a therapeutic approach to male lower urinary tract symptoms attributable to benign prostatic hyperplasia with prostatic inflammation.

8.
Prostate ; 79(11): 1226-1237, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31212363

RESUMO

BACKGROUND: We previously reported the presence of prostate-specific antigen (PSA) in the stromal compartment of benign prostatic hyperplasia (BPH). Since PSA is expressed exclusively by prostatic luminal epithelial cells, PSA in the BPH stroma suggests increased tissue permeability and the compromise of epithelial barrier integrity. E-cadherin, an important adherens junction component and tight junction regulator, is known to exhibit downregulation in BPH. These observations suggest that the prostate epithelial barrier is disrupted in BPH and E-cadherin downregulation may increase epithelial barrier permeability. METHODS: The ultra-structure of cellular junctions in BPH specimens was observed using transmission electron microscopy (TEM) and E-cadherin immunostaining analysis was performed on BPH and normal adjacent specimens from BPH patients. In vitro cell line studies using benign prostatic epithelial cell lines were performed to determine the impact of small interfering RNA knockdown of E-cadherin on transepithelial electrical resistance and diffusion of fluorescein isothiocyanate (FITC)-dextran in transwell assays. RESULTS: The number of kiss points in tight junctions was reduced in BPH epithelial cells as compared with the normal adjacent prostate. Immunostaining confirmed E-cadherin downregulation and revealed a discontinuous E-cadherin staining pattern in BPH specimens. E-cadherin knockdown increased monolayer permeability and disrupted tight junction formation without affecting cell density. CONCLUSIONS: Our results indicate that tight junctions are compromised in BPH and loss of E-cadherin is potentially an important underlying mechanism, suggesting targeting E-cadherin loss could be a potential approach to prevent or treat BPH.

9.
Sci Adv ; 5(6): eaat9206, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31214646

RESUMO

High-dimensional Bell-like states are necessary for increasing the channel capacity of the quantum protocol. However, their preparation and measurement are still huge challenges, especially for the latter. Here, we prepare an initial eight-dimensional Bell-like state based on hyperentanglement of spin and orbital angular momentum (OAM) of the first and the third orders. We design simple unitary operations to produce eight Bell-like states, which can be distinguished completely in theory among each other. We propose and illustrate a multiple projective measurement scheme composed of only linear optical elements and experimentally demonstrate that all the eight hyperentangled Bell-like states can be completely distinguished by our scheme. Our idea of manipulating the eight Bell-like states is beneficial to achieve the 3-bit channel capacity of quantum protocol, opening the door for extending applications of OAM states in future quantum information technology.

10.
J Microbiol Biotechnol ; 29(6): 913-922, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-31154745

RESUMO

Magnetic Ni0.7Co0.3Fe2O4 nanoparticles that were prepared via the rapid combustion process were functionalized and modified to obtain magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites, on which penicillin G acylase (PGA) was covalently immobilized. Selections of immobilization concentration and time of fixation were explored. Catalytic performance of immobilized PGA was characterized. The free PGA had greatest activity at pH 8.0 and 45oC while immobilized PGA's a ctivities peaked a t pH 7.5 and 45°C. Immobilized PGA had better thermal stability than free PGA at the range of 30-50°C for different time intervals. The activity of free PGA would be 0 and that of immobilized PGA still retained some activities at 60°C after 2 h. Vmax and Km of immobilized PGA were 1.55 mol/min and 0.15 mol/l, respectively. Free PGA's Vmax and Km separately were 0.74 mol/min and 0.028 mol/l. Immobilized PGA displayed more than 50% activity after 10 successive cycles. We concluded that immobilized PGA with magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO nanocomposites could become a novel example for the immobilization of other amidohydrolases.


Assuntos
Cobalto/química , Enzimas Imobilizadas/química , Nanopartículas de Magnetita/química , Nanocompostos/química , Níquel/química , Penicilina Amidase/química , Penicilina Amidase/metabolismo , Catálise , Estabilidade Enzimática , Enzimas Imobilizadas/metabolismo , Glutaral/química , Concentração de Íons de Hidrogênio , Dióxido de Silício/química , Temperatura Ambiente
11.
Neoplasia ; 21(8): 752-764, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31229879

RESUMO

ELL-associated factor 1 is a transcription elongation factor that shares significant homology and functional similarity to the androgen-responsive prostate tumor suppressor ELL-associated factor 2. EAF2 is frequently down-regulated in advanced prostate cancer and Eaf2 deletion in the mouse induced the development of murine prostatic intraepithelial neoplasia. Here we show that similar to EAF2, EAF1 is frequently down-regulated in advanced prostate cancer. Co-downregulation of EAF1 and EAF2 occurred in 40% of clinical specimens with Gleason score >7. We developed and characterized a murine model of prostate-epithelial specific deletion of Eaf1 in the prostate and crossed it with our previously generated mouse with conventional deletion of Eaf2. The prostates of Eaf1 deletion mice displayed murine prostatic intraepithelial neoplasia lesions with increased proliferation and inflammation. Combined deletion of Eaf1 and Eaf2 in the murine model induced an increased incidence in mPIN lesions characterized by increased proliferation and CD3+ T cells and CD19+ B cells infiltration compared to individual deletion of either Eaf1 or Eaf2 in the murine prostate. These results suggest that EAF1 may play a tumor suppressive role in the prostate. Cooperation between EAF1 and EAF2 may be important for prostate maintaining prostate epithelial homeostasis, and concurrent loss of these two tumor suppressors may promote prostate tumorigenesis and progression.

12.
Aging (Albany NY) ; 11(8): 2430-2446, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31039132

RESUMO

Human papillomavirus (HPV) positive head and neck cancer displayed specific transcription landscape but the underlying molecular mechanisms are not fully determined. Here, we interestingly found that HPV infection could globally elongate the 3'-untranslated regions (3'UTRs) in the majority of alternative polyadenylation (APA)-containing genes. Counterintuitively, the 3'UTR elongation does not affect their resident gene expression. Rather, they significantly increase the number of binding sites for RNA-binding proteins (RBPs) and subsequently upregulate a group of oncogenic genes by absorbing RBPs. A significant fraction of HPV affected genes are regulated through such mechanism that is 3'UTR-mediated recruitment of RBPs. As an example, we observed that HPV infection increases the length of 3'UTR of RBM25 transcript and hence recruits much more RNA binding protein including FUS and DGCR8. Consequently, in the absence of FUS and DGCR8 regulation, PD-1 was rescued and up-regulated after HPV infection. Taken together, our findings not only suggest a novel paradigm of how oncogenic viruses shape tumor transcriptome by modifying the 3'UTR, but also present a previously unrecognized layer of APA-RBP interplay in this molecular hierarchy. Modification of the pool of RBP-binding motif might expand our understandings into virus-associated carcinogenesis.

13.
Opt Lett ; 44(9): 2382-2385, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31042228

RESUMO

We design and realize a generator that can convert an orbital angular momentum (OAM) state into a vector polarization state. The generator is integrated by several commonly used optical elements and easy to make or glued. Compared with traditional interferometric ways for generating the vector optical fields, this integrated generator has compact and robust advantages and especially a high-efficiency of 87%.

14.
Int J Biol Macromol ; 134: 507-515, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31100391

RESUMO

Magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposite was prepared via the rapid combustion process, and its surface was modified to obtain amino-functionalized magnetic Ni0.5Zn0.5Fe2O4@SiO2-NH2 nanocomposite. The modified nanocomposite was loaded on graphene oxide (GO), on which penicillin G acylase (PGA) was covalently immobilized. The structure for docking was visualized between PGA and penicillin G using the PyMol program, which revealed the configuration of the active site. Selections of immobilization conditions including immobilization concentration and time of fixation, were explored. The catalytic performance of the immobilized PGA was characterized. The immobilized and free PGA had the highest activity at pH 8.0 and 45 °C. Compared with the activity of the free PGA, the activity of the immobilized PGA was affected less by pH and temperature. The immobilized PGA exhibited the high-effective activity and good stability. Vmax and Km of immobilized PGA were 0.8123 mol·min-1 and 0.0399 mol·L-1, respectively. Free PGA's Vmax and Km were 0.6854 mol·min-1 and 0.0328 mol·L-1. Immobilized PGA remained >70% in relative activity after 9 successive cycles.

15.
Medicine (Baltimore) ; 98(8): e14519, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30813154

RESUMO

To understand the risks associated with aplastic anemia (AA) in 4 cities of Zhejiang Province, China, with special focus on the joint contributions of multiple risks.Based on an Electronic Data Capture (EDC), a case control study was carried out. Data regarding socio-demographic, diseases history, living habits, and exposures to toxic substances, etc., were collected through survey questionnaires. t Test, chi-square test, or non-parametric rank sum test, and univariate and multivariate Logistic regression analysis were conducted to analyze data.The univariate logistic regression analysis results indicated that among all study participants (n = 1802), AA was associated with over 30 risks, in terms of their individual behaviors, daily and environmental exposures, diseases history, and family history. Multivariate logistic regression analysis further confirmed that the independent risks related to AA included presence of chemical factory within 3 km of living residence (odds ratio [OR] = 8.73, 95% CI: 1.42-53.74, P = .019), living in a newly decorated house/apartment (OR = 25.37, 95% CI: 4.44-144.81, P < .001), vegetarian diet (OR = 131.60, 95% CI: 3.45-5020.16, P = .009), preference of sugar (OR = 89.38, 95% CI: 7.22-1106.44, P < .001), preference of oily food (OR = 55.68, 95% CI: 5.12-605.26, P = .001), drinking lake water or pond water (OR = 58.05, 95% CI: 3.21-1049.81, P < .001), habit of staying up late (OR = 11.87, 95% CI: 3.43-41.02, P < .001), infection history (OR = 10.08, 95% CI: 2.75-36.93, P < .001). Result of receiver operating characteristic curve (ROC) analysis on the joint contribution of multiple risks indicated that AA was 13.835 times likely to occur when exposed to ≥1 risks than those exposed to 0 risks (95% CI: 9.995-19.149).Our study results demonstrated a comprehensive epidemiological pattern, in which the joint contributions of individual inherited health status, environment exposure, and individual behaviors lead to the occurrence of AA.


Assuntos
Anemia Aplástica/etiologia , Medição de Risco/métodos , Adolescente , Adulto , Idoso , Anemia Aplástica/epidemiologia , Estudos de Casos e Controles , Criança , Pré-Escolar , China/epidemiologia , Exposição Ambiental/efeitos adversos , Feminino , Comportamentos Relacionados com a Saúde/etnologia , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Inquéritos e Questionários , Adulto Jovem
16.
Prostate ; 79(8): 872-879, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30900300

RESUMO

BACKGROUND: Benign prostatic hyperplasia (BPH) is one of the major causes of lower urinary tract symptoms (LUTS), including storage LUTS such as urinary frequency and urgency. Recently, a growing number of clinical studies indicate that prostatic inflammation could be an important pathophysiological mechanism inducing storage LUTS in patients with BPH. Here we aimed to investigate whether nonbacterial prostatic inflammation in a rat model induced by intraprostatic formalin injection can lead to long-lasting bladder overactivity and changes in bladder afferent neuron excitability. METHODS: Male Sprague-Dawley rats were divided into four groups (n = 12 each): normal control group, 1-week prostatic inflammation group, 4-week inflammation group, and 8-week inflammation group. Prostatic inflammation was induced by formalin (10%; 50 µL per lobe) injection into bilateral ventral lobes of the prostate. Voiding behavior was evaluated in metabolic cages for each group. Ventral lobes of the prostate and the bladder were then removed for hematoxylin and eosin (HE) staining to evaluate inflammation levels. Continuous cystometrograms (CMG) were recorded to measure intercontraction intervals (ICI) and voided volume per micturition. Whole-cell patch clamp recordings were performed on dissociated bladder afferent neurons labeled by fluorogold injected into the bladder wall, to examine the electrophysiological properties. RESULTS: Results of metabolic cage measurements showed that formalin-treated rats exhibited significantly (P < 0.05) increases in micturition episodes/12 hours and decrease in voided volume per micturition at every time point post injection. Continuous CMG illustrated the significant ( P < 0.05) higher number of nonvoiding contractions per void and shorter ICI in formalin-treated rats compared with control rats. HE staining showed significant prostatic inflammation, which declined gradually, in prostate tissues of formalin-induced rats. In patch clamp recordings, capsaicin-sensitive bladder afferent neurons from rats with prostatic inflammation had significantly ( P < 0.05) lower thresholds for spike activation and a "multiple" firing pattern compared with control rats at every time point post injection. CONCLUSIONS: Formalin-induced prostatic inflammation can lead to long-lasting bladder overactivity in association with bladder afferent neuron hyperexcitability. This long-lasting model could be a useful tool for the study of inflammation-related aspects of male LUTS pathophysiology.

17.
Dalton Trans ; 48(16): 5193-5202, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30896012

RESUMO

Nanocomposites with a well-defined sandwich-like nanostructure were prepared via in situ growing NiCo-layered double hydroxide nanosheets with tunable Ni/Co ratios on reduced graphene oxide (rGO). Electrochemical impedance spectra and N2 adsorption/desorption isotherms confirmed that these sandwich nanostructures effectively promoted charge transport and enlarged the specific surface area. The nanocomposites with Ni : Co = 2 : 1 exhibited a maximum specific capacitance of 2130 F g-1 at 2 A g-1, excellent rate capability (72.7% retention at 15 A g-1), and cycling stability. Asymmetric supercapacitors were assembled with these nanocomposite cathodes and rGO as a negative electrode (anode), and demonstrated an energy density of 34.5 W h kg-1 at a power density of 772 W kg-1, while maintaining a capacity retention of 86.7% after 10 000 cycles at 5 A g-1. The robust electrochemical properties indicate the composites as promising electrodes for electrochemical energy storage devices.

18.
Prostate ; 79(6): 657-666, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30714180

RESUMO

BACKGROUND: DHX15 is a member of the DEAH-box (DHX) RNA helicase family. Our previous study identified it as an AR coactivator which contributes to prostate cancer progression. METHODS: We investigated DHX15 expression in castration resistant prostate cancer specimens and the influence of DHX15 on the responsiveness of prostate cancer cells to DHT stimulation. We also explored the role DHX15 played in enzalutamide resistance and the interacting domain in DHX15 with AR. DHX15 expression level in human CRPC specimens and prostate cancer specimens was detected by tissue microarray (TMA) immunostaining analysis. Colony formation assay was performed to determine the proliferation of cells treated with enzalutamide or DHT. siRNAs were used to knockdown DHX15. The interactions between DHX15 and AR were detected using co-immunoprecipitation assay. RESULTS: The expression level of DHX15 was upregulated in human CRPC specimens compared with hormone naïve prostate cancer specimens. DHX15 knockdown reduced AR sensitivity to low DHT concentrations in C4-2 cells. Inactivation of DHX15 sensitizes the enzalutamide treatment in C4-2 cells. Deletion mutagenesis indicated that DHX1 5 interacts with AR through its N terminal domain. CONCLUSIONS: These findings suggest that DHX15 contributes to prostate cancer progression. DHX15 is required for androgen receptor sensitivity to low DHT concentrations and contributes to enzalutamide resistance in C4-2 cells. Targeting DHX15 may improve the ADT treatment.

19.
Enzyme Microb Technol ; 124: 9-16, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30797484

RESUMO

Lipopolysaccharides, the major molecules in the outer membrane of Escherichia coli, affect the behavior of bacteria including outer membrane permeability, but its influence on lycopene production in E. coli has never been reported. In this study, the effects of lipopolysaccharides with different structures on lycopene biosynthesis were investigated. Firstly, the heterogenous crtEBI operon were overexpressed in 10 LPS mutant strains of E. coli W3110 (ΔwaaC, ΔwaaF, ΔwaaY, ΔwaaG, ΔwaaR, ΔwaaO, ΔwaaU, ΔwaaP, ΔwaaY and ΔwaaB), and their ability to produce lycopene were compared. ΔwaaC/pWSK29-crtEBI, ΔwaaF/pWSK29-crtEBI and ΔwaaY/pWSK29-crtEBI produced 4.19, 4.20, and 3.81 mg/g lycopene, respectively, while the control W3110/pWSK29-crtEBI produced 3.71 mg/g lycopene; the other strains produced less lycopene than the control. In order to enhance lycopene production, genes dxr, dxr, ispA, and idi were overexpressed in ΔwaaC/pWSK29-crtEBI, ΔwaaF/pWSK29-crtEBI individually or in combination, and the lycopene production in each strain was analyzed. The maximum yield of 5.39 mg/g was achieved in ΔwaaC/pWSK29-crtEBI-SRA, which is 142% higher than that in W3110/pWSK29-crtEBI. The results indicate that the length of lipopolysaccharide affects lycopene biosynthesis in E. coli, and the shorter lipopolysaccharide and higher outer membrane permeability might be beneficial to lycopene biosynthesis.


Assuntos
Escherichia coli/genética , Lipopolissacarídeos/genética , Licopeno/metabolismo , Engenharia Metabólica , Permeabilidade da Membrana Celular , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Expressão Gênica , Redes e Vias Metabólicas/genética , Mutação
20.
IUBMB Life ; 71(7): 891-900, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30724444

RESUMO

Kawasaki disease (KD) is a systemic vasculitis syndrome that leads to coronary artery aneurysm (CAA). While echocardiography is the most important imaging modality for coronary artery assessment, a specific diagnostic biomarker complementary for CAA has not been reported. We aimed to analyze the profiles of exosomal miRNAs extracted from the serum of KD patients and controls to identify candidate biomarkers for CAA. Serum samples from 39 healthy children, 42 CAA patients, 38 coronary artery dilatation (CAD) patients and 45 virus-infected patients including 24 EBV patients and 21 ADV patients were randomly selected. Next generation sequencing was used to analyze serum exosomal miRNA to detect differentially expressed miRNAs. Biomarker candidates were validated by qRT-PCR. One hundred (and) ninety-six differentially expressed miRNAs (DEMs) were detected in CAA patients and healthy children. There were 70 DEMs and 140 DEMs in CAA patients versus CAD patients, and in CAA patients versus virus-infected patients, respectively. We selected the three most upregulated (let-7i-3p, miR-17-3p, and miR-210-5p) and the three most downregulated miRNAs (miR-6743-5p, miR-1246, and miR-6834-5p) in the DEMs, which were expressed differentially in CAA patients versus healthy children, and in CAA patients versus virus-infected patients, not in virus-infected patients versus healthy children, as biomarker candidates. Excluded DEMs of CAD and virus-infected patients, let-7i-3p was detected by sequence data analysis as a biomarker candidate for CAA patients, and then validated by qRT-PCR in a larger set of clinical samples. As a biomarker candidate, let-7i-3p provides an additional means of diagnosing CAA patients. Additionally, miRNA biomarkers complement ultrasonic imaging, allowing for greater diagnostic precision. © 2019 IUBMB Life, 2019.

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