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1.
J Asian Nat Prod Res ; : 1-8, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33225748

RESUMO

A new homo-aro-cholestane glycoside parispolyside H, along with nine known compounds, were isolated from 75% ethanolic extract of the rhizome of Paris polyphylla var. chinensis. Their chemical structures were elucidated on the basic of analysis of detailed spectroscopic and physicochemical properties. In addition, the isolated compounds (1, 6-9) were evaluated for their cytotoxic activity against HepG2 human liver cancer cell lines. Among them, four known compounds (6-9) showed cytotoxicity with IC50 values ranging from 0.41 to 3.6 µM.

2.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4947-4952, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872605

RESUMO

An UPLC-MS/MS method simultaneously determining contents of quercetin-3-O-ß-D-glucose-7-O-ß-D-gentiobioside and sinapic acid in rats' plasma was firstly established and applied to study the effects of processing on pharmacokinetics of Descurainiae Semen's active constituents. Complantatoside A as internal standard,methanol used for protein precipitation,the method was validated according to the instructions of CFDA. Rats' plasma was collected after being oral administrated equal dosage of 60% ethanal extract of raw or processed Descurainiae Semen at different point of time,then the concentrations were determined to calculate pharmacokinetic parameters using DAS 3. 2. 6. And the parameters were analyzed using SPSS 23. 0,meantime the concentration-time curve was drawn.The results showed that processing had no effects on the pharmacokinetics of QGG,but could improve the absorption of sinapic acid and slow down the excretion.


Assuntos
Medicamentos de Ervas Chinesas/farmacocinética , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Ratos , Ratos Sprague-Dawley , Espectrometria de Massas em Tandem
3.
Bioorg Med Chem Lett ; 29(13): 1576-1579, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31078411

RESUMO

Basing on chromatographic separation techniques, fifteen aglycones (1-15), including two new anthraquinone aglycones (1, 2) and thirteen known compounds (3-15), were isolated from the small polar fraction of Cassia obtusefolia (petroleum ether extract). Structural elucidations were performed by 1D/2D NMR spectroscopy and mass spectrometry. The in vitro antioxidant and α-glucosidase inhibitory activities of these fifteen compounds were determined. Except compounds 12 (IC50 3.03 ±â€¯0.31 µg/mL, stronger than ascorbic acid, which IC50 was 6.48 ±â€¯2.30 µg/mL) and 13 (IC50 78.40 ±â€¯2.39 µg/mL), the free radical scavenging capacities of other compounds were weak. Compounds 4, 5, 6 and 13 exhibited inhibitory activities on α-glucosidase with IC50 values of 50.60 ±â€¯1.10, 22.57 ±â€¯0.07, 60.09 ±â€¯1.40, and 80.01 ±â€¯2.66 µg/mL separately, however, all the α-glucosidase inhibitory activities were weaker than positive control (acarbose).


Assuntos
Antioxidantes/uso terapêutico , Cassia/química , Sementes/química , alfa-Glucosidases/metabolismo , Antioxidantes/farmacologia , Estrutura Molecular
4.
Zhongguo Zhong Yao Za Zhi ; 43(15): 3145-3149, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200710

RESUMO

References and our previous experiment showed that the contents of glycosides were significantly decreased,while the contents of aglycones were significantly increased after processing of Cassiae Semen.It may be related to its glycosidases or the heating process. In order to investigate the reasons, high performance liquid chromatographic (HPLC) was used to study the effects of these two factors on contents of Cassiae Semen's main chemical components in processing. The results showed that glycoside hydrolases was present in Cassiae Semen and could rapidly hydrolyze glycosides from Cassiae Semen into aglycones in suitable temperature with sufficient water.However,it didn't show effect on contents change of main constituents in the procedure of Cassiae Semen processing.The reason for content decrease of glycosides and content increase of aglycones in processed Cassiae Semen was glycoside bond cracking to produce corresponding aglycone at high temperature.This study further provides basis for further revealing of the processing mechanism of Cassiae Semen.


Assuntos
Cassia/química , Medicamentos de Ervas Chinesas/química , Glicosídeos/química , Sementes/química , Química Farmacêutica , Cromatografia Líquida de Alta Pressão
5.
Zhongguo Zhong Yao Za Zhi ; 43(7): 1459-1466, 2018 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-29728037

RESUMO

A new flavonoid glycoside, named complanatoside C (1), and 19 known compounds (2-20) were isolated from an 95% ethanol extract of Astragali Semen by various chromatographic methods. Their structures were identified on the basis of UV, IR, NMR, MS spectroscopic data analysis, and comparison with those in literature, including fifteen flavonoid glycoside (1-15), and six other constituents (16-20), among which compounds 16-19 were isolated from this plant for the first time.


Assuntos
Astrágalo (Planta)/química , Flavonoides/análise , Glicosídeos/análise , Sementes/química , Estrutura Molecular , Compostos Fitoquímicos/análise
6.
Am J Chin Med ; 44(7): 1363-1378, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27785943

RESUMO

Shaoyao-Gancao Tang (SGT) is one of the most frequently used compound formulas in the treatment of pain-related diseases in the medical practice of traditional Chinese medicine (TCM). To investigate the anti-inflammatory and antinociceptive effects, as well as to uncover the molecular mechanism of SGT, the rat pain model of arthritis was experimentally induced by single unilateral injection of rats' left hind paw with Freund's complete adjuvant (FCA). SGT was orally administered to the rats daily at three doses individually for a period of 16 days post-model induction. Swollen degrees and pain thresholds of the rats in different groups were measured for evaluation of the anti-inflammatory and anti-nociceptive effects of SGT. Furthermore, the mRNA and protein expression levels of transient receptor potential ion channel protein vanilloid receptor 1 (TRPV1) channel as well as its calcium-mediating function in the isolated DRG neurons were further detected to provide indexes for exploration of the molecular mechanisms mediating anti-arthritic activities of SGT. As a result, FCA injection induced significant allodynia, inflammation and edema, accompanied by a significant increase in both expression and calcium-mediating function of the TRPV1 channel. Pharmacologically, oral administration of SGT at a high or middle dose demonstrated a significant relief from the above-mentioned pathological conditions in a dose-dependent manner. Simultaneously the mRNA and protein expressional levels of TRPV1 channel, as well as its calcium-mediating function, were down-regulated greatly. These findings suggest that SGT possesses a significant analgesic and anti-inflammatory effect on arthritis rats; its therapeutic activities might be achieved through reversing the elevated expression and function of TRPV1 channel evoked by FCA.


Assuntos
Artrite Experimental/complicações , Regulação para Baixo/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Dor/tratamento farmacológico , Dor/etiologia , Canais de Cátion TRPV/genética , Canais de Cátion TRPV/metabolismo , Administração Oral , Analgésicos/administração & dosagem , Analgésicos/farmacologia , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/farmacologia , Artrite Experimental/imunologia , Cálcio/metabolismo , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/administração & dosagem , Adjuvante de Freund/imunologia , Expressão Gênica/efeitos dos fármacos , Masculino , Fitoterapia , RNA Mensageiro/metabolismo , Ratos Sprague-Dawley
7.
Bioorg Med Chem Lett ; 25(23): 5520-3, 2015 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-26522947

RESUMO

Two series of biscoumarin (1-3) and dihydropyran (4-12) derivatives were successfully synthesized as new antitumor and antibacterial agents. The molecular structures of four representative compounds 2, 4, 7 and 10 were confirmed by single crystal X-ray diffraction study. The synthesized compounds (1-12) were evaluated for their antitumor activities against human intestinal epithelial adenocarcinoma cell line (HuTu80), mammary adenocarcinoma cell line (4T1) and pancreatic cancer cell line (PANC1) and antibacterial activities against one drug-sensitive Staphylococcus aureus (S. aureus ATCC 29213) strain and three MRSA strains (MRSA XJ 75302, Mu50, USA 300 LAC). The further mechanism study demonstrated that the most potent compound 1 could obviously inhibit the proliferation of cancer cells via the mechanism to induce apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Cumarínicos/síntese química , Cumarínicos/farmacologia , Piranos/síntese química , Piranos/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/química , Antineoplásicos/farmacologia , Carboplatina/síntese química , Carboplatina/química , Carboplatina/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cumarínicos/química , Cristalografia por Raios X , Humanos , Concentração Inibidora 50 , Testes de Sensibilidade Microbiana , Modelos Moleculares , Piranos/química
8.
Molecules ; 20(9): 17614-26, 2015 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-26404230

RESUMO

A novel series of biscoumarin (1-4) and dihydropyran (5-13) derivatives were synthesized via a one-pot multicomponent condensation reaction and evaluated for antibacterial and antitumor activity in vitro. The X-ray crystal structure analysis of four representative compounds, 3, 7, 9 and 11, confirmed the structures of these compounds. Compounds 1-4 showed the most potent antitumor activity among the total 13 derivatives; especially for compounds 1 and 2, they also emerged as promising antibacterial members with better antibacterial activity. In addition, the results of density functional theory (DFT) showed that compared with compounds 3 and 4, biscoumarins 1 and 2 had lower intramolecular hydrogen bonds (HB) energy in their structures.


Assuntos
Antibacterianos/síntese química , Antineoplásicos/síntese química , Cumarínicos/síntese química , Piranos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Linhagem Celular Tumoral/efeitos dos fármacos , Cumarínicos/química , Cumarínicos/farmacologia , Cristalografia por Raios X , Humanos , Ligação de Hidrogênio , Testes de Sensibilidade Microbiana , Estrutura Molecular , Piranos/química , Piranos/farmacologia , Staphylococcus aureus/efeitos dos fármacos
9.
Zhongguo Zhong Yao Za Zhi ; 40(2): 275-9, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26080558

RESUMO

OBJECTIVE: To discriminate Descurainiae Semen and Pantagirus Semen. METHOD: A high-performance liquid chromatographic method was developed to establish the fingerprint of Descurainiae Semen, and hierarchical cluster analysis (HCA) and partial least squares discriminant analysis (PLS-DA) were applied to study HPLC fingerprinting and chemical recognition. RESULT: There exists large difference of chromatographic peaks and its relative peak area of HPLC fingerprints between Descurainiae Semen and Pantagirus Semen, and after conducting statistical analysis, the result demonstrated that all samples were classified into three categories: Descurainiae Semen, Pantagirus Semen and their mixtures. CONCLUSION: The developed HPLC fingerprint combined with chemometrics can be utilized to discriminate between Descurainiae Semen and Pantagirus Semen, which was quick, simple, accurate and reliable an can provide the basis for the characterization and quality assess of Descurainiae Semen and Pantagirus Semen.


Assuntos
Brassicaceae/química , Cromatografia Líquida de Alta Pressão/métodos , Plantas Medicinais/química , Análise por Conglomerados , Análise dos Mínimos Quadrados
10.
Arch Pharm Res ; 38(6): 1054-8, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25142941

RESUMO

A phytochemical investigation on the seeds of Cassia obtusifolia led to the isolation of five new anthraquinones, including one aglycon and four glycosides. The structures were elucidated by analysis of extensive spectroscopic data and the results of acid hydrolysis. All these isolates were evaluated for their inhibitory effects against α-glucosidase, and 1 showed potent activity with IC50 value of 185 ± 15 µM.


Assuntos
Antraquinonas/farmacologia , Cassia/química , Sementes/química , Antraquinonas/isolamento & purificação , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Glicosídeos/química , Glicosídeos/isolamento & purificação , Hidrólise , Extratos Vegetais/química , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta , alfa-Glucosidases/efeitos dos fármacos
11.
Zhongguo Zhong Yao Za Zhi ; 39(24): 4699-708, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25898564

RESUMO

"Tinglizi", the ripe seed of Descurainia sophia and Lepidium apetalum, is a member of Brassicaceae (Cruciferae). Traditionally, the former is called "Nantinglizi" (Descurainiae Semen) while the latter is called "Beitinglizi" (Lepidii Semen). In the theory of traditional Chinese medicine, it has the power to purge lung-fire, relieve dyspnea, promote diuresis and reduce edema, and it is mainly indicated in a case with phlegm-fluid accumulation, cough with excessive sputum, dyspnea with being unable to lie, and general swelling. In view of its wide-spread application in clinic, a comprehensive review of Lepidii Semen and Descurainiae Semen was conducted from the following aspects: herbalogical study, variety identification, historical evolution of processing, chemical constituents, pharmacological effects, quantitative determination and toxicity which could provide reference for further research and development of "Tinglizi".


Assuntos
Brassicaceae/química , Medicamentos de Ervas Chinesas , Sementes/química , Diurese/efeitos dos fármacos , Medicamentos de Ervas Chinesas/efeitos adversos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Dispneia/tratamento farmacológico , Edema/tratamento farmacológico , Humanos , Lepidium/química , Pulmão/efeitos dos fármacos , Medicina Tradicional Chinesa , Estrutura Molecular , Componentes Aéreos da Planta/química , Plantas Medicinais
12.
Zhongguo Zhong Yao Za Zhi ; 33(4): 363-5, 2008 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-18533485

RESUMO

OBJECTIVE: To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia. METHOD: The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm. RESULT: The results showed that 5 effective components all separated well and showed good linearity. CONCLUSION: The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.


Assuntos
Antraquinonas/química , Apigenina/química , Cromatografia Líquida de Alta Pressão/métodos , Glucosídeos/química , Naftalenos/química , Senna (Planta)/química , Reprodutibilidade dos Testes , Extrato de Senna , Senosídeos , Temperatura
13.
Zhongguo Zhong Yao Za Zhi ; 33(4): 366-8, 2008 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-18533486

RESUMO

OBJECTIVE: To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia. METHOD: The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm. RESULT: A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5). CONCLUSION: The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.


Assuntos
Cassia/química , Cromatografia Líquida de Alta Pressão/métodos , Cromonas/análise , Cromonas/química , Glucosídeos/análise , Glucosídeos/química , Medicamentos de Ervas Chinesas/química , Reprodutibilidade dos Testes
14.
Zhongguo Zhong Yao Za Zhi ; 32(12): 1161-4, 2007 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17802876

RESUMO

OBJECTIVE: To explore the utility of Principal Factor Analysis (PFA) in chromatographic data for quality control. METHOD: Chromatographic fingerprints of processed root pieces of Paeonia lactiflora were determined by HPLC, the PFA was used for data processing. RESULT: The quantitative differences among different growing areas and different processing batches were found with the method. CONCLUSION: The method could be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Pinellia/química , Preparações de Plantas/análise , Plantas Medicinais/química , Ecossistema , Análise Fatorial , Temperatura Alta , Preparações de Plantas/química , Preparações de Plantas/normas , Raízes de Plantas/química , Controle de Qualidade , Reprodutibilidade dos Testes , Tecnologia Farmacêutica/métodos , Vinho
15.
Zhongguo Zhong Yao Za Zhi ; 32(11): 1054-6, 2007 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17672342

RESUMO

OBJECTIVE: To explore the utility of chromatographic data for quality control of Paeonia suffruticosa, a Chinese herbal medicine. METHOD: Chromatographic fingerprints of Paeonia suffruticosa were determined by HPLC, the clustering analysis was used for data processing. RESULT: The quantitative differences among different growing areas were found and could be used to classify herbals from different growing areas, while there seemed to be no quantitative differences for processing factor. CONCLUSION: The method could be used in quality control for monitoring herbal quality control, and the result also suggested that different growing areas may greatly affected the herbal qualities.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Paeonia/química , Plantas Medicinais/química , China , Cromatografia Líquida de Alta Pressão/instrumentação , Análise por Conglomerados , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Ecossistema , Paeonia/crescimento & desenvolvimento , Raízes de Plantas/química , Raízes de Plantas/crescimento & desenvolvimento , Plantas Medicinais/crescimento & desenvolvimento , Controle de Qualidade , Reprodutibilidade dos Testes
16.
Zhong Yao Cai ; 30(10): 1250-2, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18300495

RESUMO

OBJECTIVE: To study the chemical constituents from the leaves of Cassia angustifolia. METHODS: Compounds were isolated and repeatedly purified by chromatographic techniques on silica gel column. Their structures were elucidated by chemical and spectral methods. RESULTS: eight compounds were isolated from the leaves of Cassia angustifolia, and identified as tinnevellin glycoside(I), isorhamnetin-3-O-beta-gentiobioside(II), apigenin-6,8-di-C-glycoside(III), emodin-8-O-beta-D-glucopyranoside(IV), kaempferol(V), aloe emodin(VI), D-3-O-methylinositol(VII), sucrose(VIII). CONCLUSION: Compounds III, VII and VIII are isolated from the plant for the first time.


Assuntos
Dissacarídeos/isolamento & purificação , Glicosídeos/isolamento & purificação , Plantas Medicinais/química , Senna (Planta)/química , Antraquinonas/química , Antraquinonas/isolamento & purificação , Dissacarídeos/química , Flavonóis/química , Flavonóis/isolamento & purificação , Glucosídeos/química , Glucosídeos/isolamento & purificação , Glicosídeos/química , Quempferóis/química , Quempferóis/isolamento & purificação , Folhas de Planta/química , Sacarose/química , Sacarose/isolamento & purificação
17.
Zhongguo Zhong Yao Za Zhi ; 31(13): 1070-1, 2006 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-17048604

RESUMO

OBJECTIVE: To provide the experimental data for the quality control of Paeonia lactiflora a prepared Chinese medicinal herb. METHOD: The contents paeoniflorin among different growing areas and different processing methods methods were assayed by HPLC. RESULT: The quantitative differences of paeoniflorin content among different growing areas and different processing methods wereanalyzed quantitatively. CONCLUSION: The quantitative differences among different growing areas were not significant and the quantitative differences among different processing methods within 10%.


Assuntos
Benzoatos/análise , Hidrocarbonetos Aromáticos com Pontes/análise , Glucosídeos/análise , Paeonia/química , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , China , Cromatografia Líquida de Alta Pressão , Ecossistema , Temperatura Alta , Monoterpenos , Raízes de Plantas/química , Controle de Qualidade , Vinho
18.
Zhongguo Zhong Yao Za Zhi ; 31(11): 889-91, 2006 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17048625

RESUMO

OBJECTIVE: To provide experimental data for the quality control of processed Paeonia lactiflora, a Chinese herbal medicine. METHOD: Traditional processing of P. lactiflora was simulated, content of paeoniflorin and water extracts among different preparations were assayed by HPLC; The quantitative correlations among different processing conditions were analyzed, the effects of processing parameters on the contents of paeoniflorin and water extracts were assayed a nanalysed. RESULT AND CONCLUSION: The controlled processing parameters were correlated with covariables which showed that processing procedures was controllable, and the heating temperature was a factor impacting the content of paeoniflorin.


Assuntos
Benzoatos/análise , Hidrocarbonetos Aromáticos com Pontes/análise , Glucosídeos/análise , Paeonia/química , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Temperatura Alta , Monoterpenos , Controle de Qualidade , Temperatura
19.
Zhongguo Zhong Yao Za Zhi ; 29(9): 874-6, 2004 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-15575208

RESUMO

OBJECTIVE: To explore the utility of principal component analysis (PCA) in conjunction with the window preprocessing scheme on chromatographic data for quality control. METHOD: Chromatographic fingerprints of processed tuber of Pinellia pedatisecta were determined by HPLC, and the Window Preprocessing and PCA were used for data processing. RESULT: The quantitative differences among different growing areas and different process batches were found with this method. CONCLUSION: The method can be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.


Assuntos
Pinellia/química , Plantas Medicinais/química , China , Cromatografia Líquida de Alta Pressão/métodos , Ecossistema , Análise de Componente Principal/métodos , Controle de Qualidade
20.
Zhongguo Zhong Yao Za Zhi ; 29(10): 963-6, 2004 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-15631084

RESUMO

OBJECTIVE: To study the effects of concentration, volume and temperature of aluminium water on the change of chromatoghraphic data of processed Rhizoina Arisaematis. METHOD: Chromatographic fingerprints of processed Radix Pinellia pedatisecta were determined by HPLC, and multivariate analysis was made for data processing. RESULT AND CONCLUSION: No change on the whole chromatoghraphic data was observed at different levels of concentrition and volume of aluminium water, while there was a great change at different levels of temperature.


Assuntos
Pinellia/química , Plantas Medicinais/química , Compostos de Alúmen , Cromatografia Líquida de Alta Pressão/métodos , Temperatura Alta , Raízes de Plantas/química , Tecnologia Farmacêutica/métodos , Temperatura
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