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1.
Chin Med J (Engl) ; 132(8): 883-888, 2019 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-30958429

RESUMO

BACKGROUND: Gestational diabetes mellitus (GDM) is usually diagnosed between 24th and 28th gestational week using the 75-g oral glucose tolerance test (OGTT). It is difficult to predict GDM before 24th gestational week because fast plasma glucose (FPG) decreases as the gestational age increases. It is controversial that if FPG ≥5.1 mmol/L before 24th gestational week should be intervened or not. The aim of this study was to evaluate the value of FPG to screen GDM before 24th gestational week in women with different pre-pregnancy body mass index (BMI). METHODS: This was a multi-region retrospective cohort study in China. Women who had a singleton live birth between June 20, 2013 and November 30, 2014, resided in Beijing, Guangzhou and Chengdu, and received prenatal care in 21 selected hospitals, were included in this study. Pre-pregnancy BMI, FPG before the 24th gestational week, and one-step GDM screening with 75 g-OGTT at the 24th to 28th gestational weeks were extracted from medical charts and analyzed. The pregnant women were classified into four groups based on pre-pregnancy BMI: Group A (underweight, BMI < 18.5 kg/m), Group B (normal, BMI 18.5-23.9 kg/m), Group C (overweight, BMI 24.0-27.9 kg/m) and Group D (obesity, BMI ≥28.0 kg/m). The trend of FPG before 24th week of gestation was described, and the sensitivity and specificity of using FPG before the 24th gestational week to diagnose GDM among different pre-pregnancy BMI groups were reported. Differences in the means between groups were evaluated using independent sample t-test and analysis of variance. Pearson Chi-square test was used for categorical variables. RESULTS: The prevalence of GDM was 20.0% (6806/34,087) in the study population. FPG decreased gradually as the gestational age increased in all pre-pregnancy BMI groups until the 19th gestational week. FPG was higher in women with higher pre-pregnancy BMI. FPG before the 24th gestational week and pre-pregnancy BMI could be used to predict GDM. The incidence of GDM in women with FPG ≥5.10 mmol/L in the 19th to 24th gestational weeks and pre-pregnancy overweight or obesity was significantly higher than that in women with FPG ≥5.10 mmol/L and pre-pregnancy BMI <24.0 kg/m (78.5% [62/79] vs. 52.9% [64/121], χ = 13.425, P < 0.001). CONCLUSIONS: FPG decreased gradually as the gestational age increased in all pre-pregnancy BMI groups until the 19th gestational week. Pre-pregnancy overweight or obesity was associated with an increased FPG value before the 24th gestational week. FPG ≥5.10 mmol/L between 19 and 24 gestational weeks should be treated as GDM in women with pre-pregnancy overweight and obesity.


Assuntos
Glicemia/análise , Diabetes Gestacional/sangue , Diabetes Gestacional/diagnóstico , Jejum/sangue , Adulto , Índice de Massa Corporal , Diabetes Gestacional/epidemiologia , Feminino , Idade Gestacional , Teste de Tolerância a Glucose , Humanos , Incidência , Gravidez , Prevalência , Curva ROC , Estudos Retrospectivos
2.
J Obstet Gynaecol Res ; 44(7): 1211-1215, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29845684

RESUMO

OBJECTIVE: To identify the vary of obstetric outcomes by the severity of pulmonary hypertension (PH). METHODS: A retrospective study involved pregnancies with PH in one certain academic institution. Total 78 pregnancies with PH were classified into three groups according the mean pulmonary arterial systolic pressure: mean pulmonary arterial systolic pressure 30-49 mm Hg (mild-PH group), mean pulmonary arterial systolic pressure 50-69 mm Hg (moderate-PH group) and mean pulmonary arterial systolic pressure ≥ 70 mm Hg (severe-PH group). The clinical features and obstetric outcomes were described and compared among three groups. RESULTS: The incidence of PH was 0.23%, including 57 cases in mild-PH group, 10 cases in moderate-PH group and 11 cases in severe-PH group. Meanwhile, pregnancies with mild PH present NYHA class I/II (P < 0.001). General anesthesia (P < 0.001) and advanced intensive care (P = 0.011) were supplied for pregnancies with severe PH. There was no significant difference in rate of caesarean delivery. Furthermore, severity of PH increased the risk of neonatal death (P = 0.040). CONCLUSION: The severity of PH seems to be a poor prognosis of neonatal death. Intensive care should be provided for pregnancies with severe PH.


Assuntos
Hipertensão Pulmonar/epidemiologia , Complicações na Gravidez/epidemiologia , Resultado da Gravidez/epidemiologia , Adulto , Feminino , Humanos , Gravidez , Estudos Retrospectivos , Adulto Jovem
3.
Int J Clin Exp Med ; 8(10): 19496-500, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26770599

RESUMO

BACKGROUND: The usage of Traditional Chinese Medicine (TCM) during pregnancy is very common for Chinese people. However, there are only a few studies relevant to the usage of TCM during pregnancy-providing very little knowledge on the benefits or harmful effects of these medicines to pregnant women or the fetus. OBJECTIVES: The purpose of this study is to survey the current situation of TCM usage during pregnancy, and to explore the factors that affect the use of TCM. METHODS: Data was collected from pregnant patients who delivered in our hospital, from March 2012 to August 2012. All patients who agreed to join this study were asked to fill-up a questionnaire. RESULTS: 1,010 patients were willing to participate in this study. 11.2% (113, 1,010) of patients used at least one kind of TCM during pregnancy. The average TCM usage is 2.1 species. The most commonly used drugs are Antai pill (39 cases, 34.5%), Xiaochaihu granules (31 cases, 27.4%), and Shengxuening tablets (23 cases, 20.4%). Patients who smoke during pregnancy (OR=1.8, 95% CI: 0.7~5.1), drink during pregnancy (OR=1.6, 95% CI: 0.4~6.1), have been hospitalized during pregnancy (OR=4.8, 95% CI: 2.9~7.9), have pregnancy complications (OR=2.7, 95% CI: 1.5~4.9) and those who are infertile (OR=2.6, 95% CI: 1.4~4.8) have higher chances of using TCM during pregnancy. CONCLUSIONS: Pregnant women commonly use TCM during pregnancy. Pregnant women who smoke, drink, hospitalized during pregnancy, experience pregnancy complications and infertility are more likely to use TCM.

4.
Zhonghua Yi Xue Za Zhi ; 93(33): 2646-9, 2013 Sep 03.
Artigo em Chinês | MEDLINE | ID: mdl-24360045

RESUMO

OBJECTIVE: To separately evaluate the embryo implantation rate (IR), clinical pregnancy rate and multiple pregnancy rate between women <38 years old or ≥ 38 years old in fresh or frozen thawed embryo transfer cycles, or in single or double embryo transfer cycles. METHODS: A total of 7465 single or double embryo transfer cycles between January 2010 and December 2012 at Affiliated Sir Run Run Shaw Hospital, Zhejiang University were analyzed. There were 1546 single embryo transfer cycles and 5919 double embryo transfer cycles; 2447 fresh embryo transfer cycles and 5018 frozen-thawed embryo transfer cycles. RESULTS: (1) Regardless of whether fresh or frozen-thawed embryo was used for transfer, there was no significant difference in embryo implantation rate between single and double embryo transfer groups in women <38 years. The clinical pregnancy rate significantly increased if double embryos were replaced (fresh embryo transfer, 53.3% vs 35.5%; frozen-thawed embryo transfer, 59.8% vs 38.0%, P < 0.01). Twin pregnancy rate also significantly increased (fresh embryo transfer, 28.7% vs 0.6%; frozen-thawed embryo transfer, 31.1% vs 3.0%, P < 0.01); (2) embryo implantation rate in fresh embryo transfer significantly increased in women ≥ 38 years old between groups of single or double embryo transfer (20.3% vs 9.5%, P < 0.05). No significant difference existed in IR for frozen-thawed embryo transfer. The clinical pregnancy rate significantly increased (P < 0.01) if double embryos were replaced (fresh embryo transfer, 33.2% vs 9.5%; frozen-thawed embryo transfer, 39.0% vs 21.1%, P < 0.01). Twin pregnancy rate was 19.4% in fresh embryo transfers versus 13.4% in frozen-thawed embryo transfers. There was no twin pregnancy in single embryo transfers. CONCLUSION: Compared with single embryo transfer in women < 38 years, double embryo transfer can significantly increase the rates of clinical pregnancy nd twin pregnancy. Decision is made after thorough consultations and single embryo transfer is justifiable. Double embryo transfer is indicated for women ≥ 38 years.


Assuntos
Fatores Etários , Criopreservação , Transferência Embrionária , Resultado da Gravidez , Adulto , Feminino , Humanos , Gravidez , Taxa de Gravidez , Gravidez Múltipla , Estudos Retrospectivos
5.
Diabetes Care ; 36(7): 2038-40, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23536582

RESUMO

OBJECTIVE: To evaluate the usefulness of a fasting plasma glucose (FPG) at 24-28 weeks' gestation to screen for gestational diabetes mellitus (GDM). RESEARCH DESIGN AND METHODS: The medical records and results of a 75-g 2-h oral glucose tolerance test (OGTT) of 24,854 pregnant women without known pre-GDM attending prenatal clinics in 15 hospitals in China were examined. RESULTS: FPG cutoff value of 5.1 mmol/L identified 3,149 (12.1%) pregnant women with GDM. FPG cutoff value of 4.4 mmol/L ruled out GDM in 15,369 (38.2%) women. With use of this cutoff point, 12.2% of patients with mild GDM will be missed. The positive predictive value is 0.322, and the negative predictive value is 0.928. CONCLUSIONS: FPG at 24-28 weeks' gestation could be used as a screening test to identify GDM patients in low-resource regions. Women with an FPG between ≥4.4 and ≤5.0 mmol/L would require a 75-g OGTT to diagnose GDM. This would help to avoid approximately one-half (50.3%) of the formal 75-g OGTTs in China.


Assuntos
Diabetes Gestacional/sangue , Jejum/sangue , Glicemia/metabolismo , China/epidemiologia , Diabetes Gestacional/epidemiologia , Feminino , Teste de Tolerância a Glucose , Humanos , Gravidez
6.
J Obstet Gynaecol Res ; 39(1): 132-8, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22690802

RESUMO

AIM: To investigate the relationship between maternal overweight and fetal insulin resistance. MATERIAL AND METHODS: Nineteen overweight and 30 lean pregnant women were recruited in the present study. Maternal and fetal insulin resistance were determined by measuring sex hormone binding globulin (SHBG) concentrations in maternal venous or umbilical cord serum, respectively. Maternal age, gestational age, height, pre-gravidity weight, pre-partum weight, as well as fetal gender, birth weight, birth height, and head circumference were collected as clinical data. RESULTS: Fetuses of overweight mothers had larger birth weight (3.58±0.55kg vs 3.32±0.42, adjusted P=0.006) and lower SHBG concentrations (26.64±3.65 vs 34.36±7.84, adjusted P=0.007) than those of lean mothers after values were adjusted for potential cofactors. Fetal SHBG level was negatively correlated with pre-gravidity body mass index (R=-0.392, adjusted P=0.025) and weight gain during pregnancy (R=-0.332, adjusted P=0.026) even with adjustment for potential cofactors. Among the 29 pregnant women with gestational diabetes mellitus, the overweight mothers had higher H1AC levels than their lean counterparts (6.47±0.44 vs 5.74±0.52, adjusted P=0.004). CONCLUSION: Intrauterine insulin resistance is more prominent in fetuses of overweight mothers, an effect that is decreased by weight gain control during pregnancy.


Assuntos
Peso ao Nascer/fisiologia , Diabetes Gestacional/metabolismo , Feto/metabolismo , Resistência à Insulina/fisiologia , Sobrepeso/metabolismo , Adulto , Índice de Massa Corporal , Feminino , Idade Gestacional , Teste de Tolerância a Glucose , Humanos , Recém-Nascido , Gravidez
7.
Diabetes Care ; 36(3): 586-90, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23193214

RESUMO

OBJECTIVE: To evaluate the value of fasting plasma glucose (FPG) value in the first prenatal visit to diagnose gestational diabetes mellitus (GDM). RESEARCH DESIGN AND METHODS: Medical records of 17,186 pregnant women attending prenatal clinics in 13 hospitals in China, including the Peking University First Hospital (PUFH), were examined. Patients with pre-GDM were excluded; data for FPG at the first prenatal visit and one-step GDM screening with 75-g oral glucose tolerance test (OGTT) performed between 24 and 28 weeks of gestation were collected and analyzed. RESULTS: The median ± SD FPG value was 4.58 ± 0.437. FPG decreased with increasing gestational age. FPG level at the first prenatal visit was strongly correlated with GDM diagnosed at 24-28 gestational weeks (χ(2) = 959.3, P < 0.001). The incidences of GDM were 37.0, 52.7, and 66.2%, respectively, for women with FPG at the first prenatal visit between 5.10 and 5.59, 5.60 and 6.09, and 6.10-6.99 mmol/L. The data of PUFH were not statistically different from other hospitals. CONCLUSIONS: Pregnant women (6.10 ≤ FPG < 7.00 mmol/L) should be considered and treated as GDM to improve outcomes; for women with FPG between 5.10 and 6.09 mmol/L, nutrition and exercise advice should be provided. An OGTT should be performed at 24-28 weeks to confirm or rule out GDM. Based on our data, we cannot support an FPG value ≥5.10 mmol/L at the first prenatal visit as the criterion for diagnosis of GDM.


Assuntos
Glicemia/metabolismo , Diabetes Gestacional/diagnóstico , Jejum/sangue , China , Feminino , Idade Gestacional , Humanos , Gravidez , Cuidado Pré-Natal
8.
PLoS One ; 7(9): e44701, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22970290

RESUMO

OBJECTIVE: To investigate the placental proteome differences between pregnant women complicated with gestational diabetes mellitus (GDM) and those with normal glucose tolerance (NGT). METHODS: We used two-dimensional electrophoresis (2DE) to separate and compare placental protein levels from GDM and NGT groups. Differentially expressed proteins between the two groups were identified by MALDI-TOF/TOF mass spectrometry and further confirmed by Western blotting. The mRNA levels of related proteins were measured by realtime RT-PCR. Immunohistochemistry (IHC) was performed to examine the cellular location of the proteins expressed in placenta villi. RESULTS: Twenty-one protein spots were differentially expressed between GDM and NGT placenta villi in the tested samples, fifteen of which were successfully identified by mass spectrometry. The molecular functions of these differentially expressed proteins include blood coagulation, signal transduction, anti-apoptosis, ATP binding, phospholipid binding, calcium ion binding, platelet activation, and tryptophan-tRNA ligase activity. Both protein and mRNA levels of Annexin A2, Annexin A5 and 14-3-3 protein ζ/δ were up-regulated, while the expression of the Ras-related protein Rap1A was down-regulated in the GDM placenta group. CONCLUSION: Placenta villi derived from GDM pregnant women exhibit significant proteome differences compared to those of NGT mothers. The identified differentially expressed proteins are mainly associated with the development of insulin resistance, transplacental transportation of glucose, hyperglucose-mediated coagulation and fibrinolysis disorders in the GDM placenta villi.


Assuntos
Coagulação Sanguínea , Fibrinólise , Resistência à Insulina , Placenta/metabolismo , Proteínas/metabolismo , Adulto , Eletroforese em Gel Bidimensional , Feminino , Humanos , Imuno-Histoquímica , Gravidez , Proteínas/genética , Proteínas/isolamento & purificação , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrometria de Massas em Tandem
9.
Menopause ; 19(7): 791-8, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22278344

RESUMO

OBJECTIVE: Estrogen therapy is, to date, the most effective treatment of menopausal syndrome and also has a favorable effect on lipid profiles. Because of its potential adverse effects, however, a more acceptable alternative therapy needs to be identified. This study examines the effect of soy germ isoflavones on menopausal symptoms and serum lipids. METHODS: Ninety early postmenopausal Chinese women, aged 45 to 60 years, were randomly assigned to three treatment groups (30 each) receiving daily doses of 0 (placebo), 84, and 126 mg of soy germ isoflavones. Hot flush frequency, Kupperman scores, serum 17ß-estradiol, follicle-stimulating hormone, luteinizing hormone, and serum lipids, including triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, apolipoprotein A-I, and apolipoprotein B100, were assessed at baseline and at 12 and 24 weeks after treatment. RESULTS: Both the frequency of hot flushes and the Kupperman index score decreased in all three treatment groups during the intervention period, but the percentage decreases in both were significantly greater in the two isoflavone groups (44.3 ± 19.1 and 57.8 ± 37.4 [84 mg isoflavones]; 48.5 ± 27.2 and 56.7 ± 26.7 [126 mg isoflavones]) than in the placebo group (27.8 ± 15.5 and 34.6 ± 46.2; p < 0.01). There was no significant difference in the changes in estradiol, follicle-stimulating hormone, and luteinizing hormone among the three treatment groups during the study, and no significant differences were observed in the lipid components. CONCLUSIONS: A daily supplement of 84 or 126 mg soy germ isoflavones may improve menopausal symptoms, although neither dose was found to affect lipid profiles in early postmenopausal Chinese women after 24 weeks of treatment. The favorable effects are unlikely to be associated with female hormones.


Assuntos
Fogachos/tratamento farmacológico , Isoflavonas/administração & dosagem , Lipídeos/sangue , Pós-Menopausa , Soja/química , China , Estradiol/sangue , Estrogênios/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Hormônio Luteinizante/sangue , Pessoa de Meia-Idade , Fitoestrógenos/administração & dosagem , Índice de Gravidade de Doença , Método Simples-Cego , Resultado do Tratamento
10.
Proteome Sci ; 9(1): 24, 2011 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-21554704

RESUMO

BACKGROUND: High abundance protein depletion is a major challenge in the study of serum/plasma proteomics. Prior to this study, most commercially available kits for depletion of highly abundant proteins had only been tested and evaluated in adult serum/plasma, while the depletion efficiency on umbilical cord serum/plasma had not been clarified. Structural differences between some adult and fetal proteins (such as albumin) make it likely that depletion approaches for adult and umbilical cord serum/plasma will be variable. Therefore, the primary purposes of the present study are to investigate the efficiencies of several commonly-used commercial kits during high abundance protein depletion from umbilical cord serum and to determine which kit yields the most effective and reproducible results for further proteomics research on umbilical cord serum. RESULTS: The immunoaffinity based kits (PROTIA-Sigma and 5185-Agilent) displayed higher depletion efficiency than the immobilized dye based kit (PROTBA-Sigma) in umbilical cord serum samples. Both the PROTIA-Sigma and 5185-Agilent kit maintained high depletion efficiency when used three consecutive times. Depletion by the PROTIA-Sigma Kit improved 2DE gel quality by reducing smeared bands produced by the presence of high abundance proteins and increasing the intensity of other protein spots. During image analysis using the identical detection parameters, 411 ± 18 spots were detected in crude serum gels, while 757 ± 43 spots were detected in depleted serum gels. Eight spots unique to depleted serum gels were identified by MALDI- TOF/TOF MS, seven of which were low abundance proteins. CONCLUSIONS: The immunoaffinity based kits exceeded the immobilized dye based kit in high abundance protein depletion of umbilical cord serum samples and dramatically improved 2DE gel quality for detection of trace biomarkers.

11.
Zhonghua Fu Chan Ke Za Zhi ; 46(1): 28-31, 2011 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-21429431

RESUMO

OBJECTIVE: To investigate whether no asphyxia neonates with intrauterine distress are complicated with myocardial injury and determine the sensitive biochemical diagnostic parameters. METHODS: A total of 89 neonates born in the First Affiliated Hospital of Sun Yat-sen University from July 2009 to December 2009 were enrolled. Fifty-three fetal distress cases with Apgar score > 7 at 1 and 5 minutes were enrolled in the study group; while the rest 36 healthy neonates, whose Apgar score = 10 at 1 and 5 minutes, were the control group. Umbilical artery blood samples of all cases were collected for blood gas analysis and biochemical measurement. RESULTS: (1) pH (7.23 ± 0.07) and BE [(-4.8 ± 3.0) mmol/L] in the study group were significantly lower than pH (7.31 ± 0.03) and BE [(-2.1 ± 1.5) mmol/L ] in the control group(P < 0.05). The lactic acid of study group [(5.2 ± 2.3) mmol/L] was higher than that of the control group [(2.3 ± 1.1) mmol/L], and the difference was significant (P < 0.01). However, there was no significant difference between the two groups in PaO2 [(16.2 ± 7.9) mm Hg (1 mm Hg = 0.133 kPa) vs. (17.5 ± 6.7) mm Hg] and PaCO2 [(54.0 ± 11.2) mm Hg vs. (48.5 ± 5.4) mm Hg; P > 0.05]. (2) The level of CK-MB in neonates with fetal distress[ (48 ± 59) U/L] was significantly higher than that of healthy neonates [(36 ± 27) U/L]. However, no significant difference was found in CK [(194 ± 73) U/L vs. (162 ± 95) U/L] and BNP levels [(519 ± 309) ng/L vs. (481 ± 216) ng/L; P > 0.05]. (3) Spearman rank correlation analysis showed that CK-MB level was negatively correlated with pH (r = -0.296, P < 0.05) and BE (r = -0.318, P < 0.05) of umbilical artery blood, while BNP level was positively correlated with umbilical lactic acid (r = 0.278, P < 0.05). No correlation was found between other parameters (P > 0.05). CONCLUSIONS: Intrauterine distress without neonatal asphyxia had effect on fetal myocardial injury. CK-MB can be used as a sensitive parameter for monitoring the development of myocardial injury. The severity of myocardial injury was related to fetal acidosis.


Assuntos
Creatina Quinase Forma MB/sangue , Creatina Quinase/sangue , Sofrimento Fetal/complicações , Traumatismos Cardíacos/enzimologia , Miocárdio/enzimologia , Artérias Umbilicais/química , Adulto , Índice de Apgar , Biomarcadores/sangue , Gasometria , Estudos de Casos e Controles , Feminino , Humanos , Concentração de Íons de Hidrogênio , Recém-Nascido , Isoenzimas/sangue , Masculino , Miocárdio/patologia , Peptídeo Natriurético Encefálico/sangue
12.
J Obstet Gynaecol Res ; 36(6): 1214-21, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21114574

RESUMO

AIM: To establish an improved noninvasive fluorescent animal model for endometriosis. MATERIAL AND METHODS: Adenovirus encoding enhanced green fluorescent protein (Ad-eGFP) was used to transfect primary culture endometrial glandular cells and stromal cells (purified cell transfection and mixed injection, Group 1) as well as endometrial fragments (tissues transfection and injection, Group 2). Transfection results were compared between the cells and tissues in vitro. The GFP-transfected cells suspension of Group 1 or endometrial fragments of Group 2, with similar weight, were injected into nude mice subcutaneously and noninvasively observed every 5 days until day 15 (Subgroup 1, N = 5), day 20 (Subgroup 2, N = 5) or day 25 (Subgroup 3, N =11). The positive rates and duration times of the fluorescent lesions were calculated. RESULTS: After 18 h of incubation, glandular cells and stromal cells all had higher GFP-positive rates. In vivo imaging showed that the GFP positive rates of Group 1 were significantly higher than those of Group 2. The fluorescent-positive durations of Groups 1 and 2 were 23.636 ± 4.523 days and 5.909 ± 5.394 days, respectively (P < 0.001). In vivo analysis demonstrated that on days 15, 20, and 25, there were more typical lesions and fluorescent-positive lesions formed in Group 1 and that the lesion weight in Group 1 was greater. The structures of the lesions were all identified as human origin. CONCLUSION: A noninvasive animal model for endometriosis created by subcutaneous injection of an Ad-eGFP-transfected endometrial glandular and stromal cells suspension had higher a positive rate, longer duration time of fluorescent imaging and greater lesion weight.


Assuntos
Modelos Animais de Doenças , Endometriose , Adenoviridae , Adulto , Animais , Feminino , Vetores Genéticos , Proteínas de Fluorescência Verde/genética , Humanos , Injeções Subcutâneas , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Transfecção , Adulto Jovem
13.
Zhonghua Liu Xing Bing Xue Za Zhi ; 31(8): 929-32, 2010 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-21162999

RESUMO

OBJECTIVE: To investigate the perinatal complications, birth defects and growth of children conceived through intracytoplasmic sperm injection (ICSI). METHODS: A total of 575 children conceived by ICSI in our reproductive medical center, were studied. The follow-up study would include items as pregnant complications, neonatal complications, birth defects in perinatal period, subsequently detected birth defects, body weight and body length/height growth. RESULTS: Prematurity and low birth weight of ICSI children were higher in the multiple births than in the singleton births. The rates of materal gestational hypertension, neonatal asphyxia, respiratory distress syndrome, infection diseases were higher in the multiple pregnancies than in the singleton pregnancies (P < 0.05). Eleven ICSI children had died. Ten of them died in the neonatal period and they were preterm infants. One fullterm singleton ICSI child died of hepatoblastoma at the age of 2. The rate of birth defects in perinatal period was higher in ICSI children of multiple pregnancies than in the general population (P < 0.05). The body weight and body length/height of most ICSI children had obtained the standard range between 1 to 3 year-olds. CONCLUSION: The higher rates of perinatal complications in ICSI children were closely related to multiple pregnancies.


Assuntos
Resultado da Gravidez , Injeções de Esperma Intracitoplásmicas , Criança , Seguimentos , Humanos , Recém-Nascido de Baixo Peso , Gravidez Múltipla
14.
Zhonghua Fu Chan Ke Za Zhi ; 45(7): 492-6, 2010 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-21029599

RESUMO

OBJECTIVE: To investigate safety and efficacy of dinoprostone suppositories (0.8 mm) used in cervical ripening and labor induction in women with term premature rupture of the membranes. METHODS: One hundred women of term monocyesis with premature rupture of the membranes, head presentation, bishop score less than 6 (test group) and 180 women with intact fetal membranes (control group) were enrolled into this multicenter, prospective clinical study. The vaginal delivery system was inserted into the posterior fornix, and the patients were recumbent for 2 hours after insertion. The interval time from using dinoprostone suppositories to uterine contraction, to labor and delivery were recorded. The following index were also recorded and compared, including the mean inserted time of dinoprostone suppositories, fetal heart beat, meconium stained amniotic fluid, hyperstimulation of uterus and the other complications, mode of delivery, stage of labor, postpartum hemorrhage, status of neonates. RESULTS: Three cases in test group and 23 cases in control group weren't in labor within 24 hours. The rate of labor within 24 hours in test group was significant higher than that in control group (97.0% vs. 87.2%, P < 0.01). It was observed that 73 cases undergoing vaginal deliveries (75.3%, 73/97) and 24 cases undergoing cesarean section deliveries (24.7%, 24/97)in test group and 107 cases undergoing vaginal delivery (68.2%, 107/157) and 50 cases undergoing cesarean section delivery (31.8%, 50/157) in control group, when compared the rate of vaginal or cesarean section deliveries between two group, it didn't reach statistical difference (P > 0.05). It had no significant difference in the interval time from using dinoprostone suppositories to labor starting and the mean inserted time and the total labor time between two groups (P > 0.05). The incidence of uterine tachysystole was 11.3% (11/97) in test group and 19.1% (30/157) in control group (P > 0.05), which did not reach statistical difference (P > 0.05). There wasn't neonatal asphyxia in both groups. CONCLUSION: It was safe and efficient to use dinoprostone suppositories for cervical ripening and induction of term pregnancy with premature rupture of the membranes, however, monitoring should be intensified.


Assuntos
Maturidade Cervical/efeitos dos fármacos , Dinoprostona/uso terapêutico , Ruptura Prematura de Membranas Fetais/terapia , Trabalho de Parto Induzido/métodos , Ocitócicos/uso terapêutico , Administração Intravaginal , Adulto , Cesárea , Parto Obstétrico , Dinoprostona/administração & dosagem , Dinoprostona/efeitos adversos , Feminino , Humanos , Ocitócicos/administração & dosagem , Ocitócicos/efeitos adversos , Gravidez , Resultado da Gravidez , Terceiro Trimestre da Gravidez , Estudos Prospectivos , Supositórios , Fatores de Tempo , Contração Uterina/efeitos dos fármacos
15.
J Agric Food Chem ; 58(1): 642-9, 2010 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-20000702

RESUMO

Fatty acid composition and distribution of human milk fat (HMF), from mothers over different lactating periods in Guangzhou, China, were analyzed. The universal characteristics were consistent with previously reported results although the fatty acid content was within a different range and dependent on the local population (low saturated fatty acid and high oleic acid for Guangdong mothers' milk fat). Based on the composition of the total and sn-2 fatty acids of mature milk fat, an efficient evaluation model was innovatively established by adopting the "deducting score" principle. The model showed good agreement between the scores and the degree of similarity by assessing 15 samples from different sources including four samples of HMF, eight samples of human milk fat substitutes (HMFSs) and infant formulas, and three samples of fats and oils. This study would allow for the devolvement of individual human milk fat substitutes with different and specific fatty acid compositions for local infants.


Assuntos
Substitutos da Gordura/análise , Leite Humano/química , China , Ácidos Graxos/análise , Feminino , Humanos , Modelos Estatísticos
16.
Zhonghua Fu Chan Ke Za Zhi ; 44(11): 801-4, 2009 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-20079027

RESUMO

OBJECTIVE: To evaluate the influences of abnormal glucose challenge test(GCT) on pregnancy outcomes and neonatal anthropometric data in women with normal oral glucose tolerance test(OGTT). METHODS: Totally 214 women who delivered in the First Affiliated Hospital of Sun Yat-sen University from November 2006 to December 2007 were enrolled. 50 g GCT was performed at 24-28 weeks of gestation and 75 g OGTT would be followed if GCT >/= 7.8 mmol/L. Those patients, whose OGTT results below the following criteria (5.3 mmol/L, 10.0 mmol/L, 8.6 mmol/L, 7.8 mmol/L), were classified as normal OGTT. Altogether, 116 of the 214 women with abnormal GCT and normal OGTT were collected as the study group and the rest 98 women with normal GCT as the control group. The pregnant outcomes of the two groups were analyzed. The neonatal anthropometry, including birth weight, body length, head circumference and shoulder circumference, were recorded. Other neonatal anthropometric data, such as upper arm circumference, tricep skinfold thickness and hypodermic fat thickness of abdomen were measured by a tape measure within 24 hours after birth. RESULTS: (1) Pregnant outcomes: No significant difference was found in the rate of assisted vaginal delivery, polyhydramnios, premature rupture of membranes and fetal distress between the study and control group [10.3% (12/116) vs 4% (4/98), 5.2% (6/116) vs 10% (10/98), 13.8% (16/116) vs 17% (17/98), 20.7% (24/116) vs 13% (13/98), P > 0.05, respectively], but the rate of cesarean section, spontaneous vaginal delivery and large for gestational age babies in the study group were different from those of the control [72.4% (84/116) vs 51% (51/98), 17.2% (20/116) vs 45% (44/98), 25.9% (30/116) vs 6% (6/98), P < 0.05, respectively]. (2) Neonatal anthropometry: The birth weight of the study group was significantly higher than that of the control group [(3.4 +/- 0.4) kg vs (3.3 +/- 0.4) kg, P < 0.05], but no significant difference was shown in any other neonatal anthropometric results between the study and control group, including body length [(49.9 +/- 1.3) cm vs (49.7 +/- 1.4) cm], head circumference [(33.4 +/- 1.5) cm vs (33.8 +/- 1.7) cm], shoulder circumference [(35.4 +/- 2.3) cm vs (35.0 +/- 2.3) cm], upper arm circumference [(11.0 +/- 0.7) cm vs (10.9 +/- 0.8) cm], tricep skinfold thickness [(9.7 +/- 1.0) mm vs (9.9 +/- 1.4) mm] and hypodermic fat thickness of abdomen [(7.2 +/- 1.2) mm vs (7.2 +/- 1.0) mm; all P > 0.05]. CONCLUSION: Women with abnormal GCT alone may have no significant influences on neonatal anthropometric data, but might have more cesarean section, large for gestational age babies, and neonatal birth weight than those women with normal GCT.


Assuntos
Diabetes Gestacional , Teste de Tolerância a Glucose , Antropometria , Peso ao Nascer , Feminino , Glucose , Humanos , Gravidez , Resultado da Gravidez
17.
Chin Med J (Engl) ; 121(8): 696-700, 2008 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-18701021

RESUMO

BACKGROUND: Women with a history of gestational diabetes mellitus (GDM) are at higher risk of future development of diabetes. This study investigated the risk factors associated with early postpartum abnormal glucose regulation (AGR) among Chinese women with a history of GDM. METHODS: A total of 186 women with a history of GDM were screened for early postpartum AGR at 6-8 weeks after delivery. Those with AGR were given lifestyle intervention therapy and reevaluated in 6-12 months. The demographic, anthropometric, prenatal and delivery data were recorded. The plasma high-sensitivity C-reactive protein (HsCRP) and lipid concentration were measured, and insulin secretion were analyzed. Insulinogenic index Deltains30'/DeltaBG30', the homeostasis model assessment index (HOMA)-B, and HOMA-IR were calculated. Multiple regression analysis was performed to identify the risk factors. RESULTS: Of the GDM women 28.0% (52/186) had AGR at 6-8 weeks after delivery; 45.2% (17/40) of these AGR women reminded abnormal after 6-12 month lifestyle intervention. Compared to the women who reverted to normal, women with consistent AGR showed significantly lower fasting insulin concentration, lower Deltains30'/DeltaBG30' as well as lower HOMA-B. No significant differences in age, body mass index (BMI), waist circumference, blood pressure, lipid level, HsCRP and HOMA-IR were observed between the two groups. Pre-pregnancy BMI = 25 kg/m(2), fasting glucose level = 5.6 mmol/L and/or 75 g oral glucose tolerance test (OGTT) 2 hours glucose level = 11.1 mmol/L during pregnancy were predictors for the AGR at 6-8 weeks after delivery. Deltains30'/DeltaBG30 = 1.05 was a significant risk contributor to the consistent early postpartum AGR. CONCLUSION: There is a high incidence of early postpartum AGR among Chinese woman with prior GDM. Beta-cell dysfunction, rather than insulin resistance or inflammation, is the predominant contributor to the early onset and consistent AGR after delivery.


Assuntos
Diabetes Mellitus Tipo 2/etiologia , Diabetes Gestacional , Células Secretoras de Insulina/fisiologia , Transtornos Puerperais/etiologia , Adulto , Grupo com Ancestrais do Continente Asiático , China , Feminino , Humanos , Gravidez , Fatores de Risco
18.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 24(5): 564-6, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-17922428

RESUMO

OBJECTIVE: To investigate the relationship between microdeletion of azoospermia factor (AZF) and male infertility. METHODS: Multiplex PCR was used to detect Y chromosome microdeletion in AZFa, AZFb and AZFc in 103 cases of idiopathic azoospermia, 72 cases of severe idiopathic oligozoospermia, and 60 healthy male controls. RESULTS: No microdeletion was found in 60 controls. Y chromosome microdeletion was found in 19 of 175 azoospermia patients, the total prevalence rate of microdeletion was 10.9%. There were 15 cases (11 for azoospermia, 4 for severe oligozoospermia) in AZFc (8.6%), 3 cases (1 for azoospermia, 2 for severe oligozoospermia) in AZFb+c (1.7%), 1 case (azoospermia) in AZFa+b+c (0.6%). According to statistics, the difference of microdeletion rate between two groups was significant(P < 0.01). CONCLUSION: Y chromosome microdeletions is an important reason of azoospermia. Screening of Y chromosome microdeletions for azoospermia patients before intracytoplasmic sperm injection treatment is essential.


Assuntos
Deleção Cromossômica , Cromossomos Humanos Y/genética , Testes Genéticos , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/genética , Proteínas de Plasma Seminal/genética , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Azoospermia/diagnóstico , Azoospermia/genética , Estudos de Casos e Controles , China , Feminino , Loci Gênicos , Humanos , Masculino , Pessoa de Meia-Idade , Oligospermia/diagnóstico , Oligospermia/genética
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