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1.
Biosaf Health ; 2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-35005601

RESUMO

The Coronavirus Disease 2019 (COVID-19) is still causing a wide range of infections and deaths due to the high variability of the SARS-CoV-2 virus. Therefore, it is necessary to establish a reliable and convenient pseudovirus-based neutralization assay to develop drug targeted variants of SARS-CoV-2. Based on the HIV-1 backbone, we generated a high titer luciferase (Luc)-expressing pseudovirus packaging system. Three dominant S mutant substitution pseudovirus were also established and identified compared to wide type in hACE2-overexpressing HEK-293T cells (293T-ACE2 cells). Compared to serine protease inhibitor camostat mesylate, the cysteine protease inhibitor E-64d could significantly block all SARS-CoV-2 mutant S pseudovirus infection in 293T-ACE2 cells. Furthermore, the neutralization ability of two antibodies targeted receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) was evaluated, which showed different inhibition dose-effect curves among four types of S pseudovirus. Overall, we developed a pseudovirus-based neutralization assay for SARS-CoV-2, which would be readily adapted to SARS-CoV-2 variants for evaluating antibodies.

2.
Asian J Surg ; 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-35012854

RESUMO

BACKGROUND: This study aimed to explore the survival and recurrences of stage I colorectal cancer (CRC) patients. Through the analysis of the results of preoperative serological values, we seek to find factors associated with the survival and recurrence of patients with stage I CRC. METHODS: We retrospectively enrolled patients from 2012 January to 2015 April. Survival rates were calculated with the Kaplan-Meier method and survival curves were compared using the log-rank test. The independent prognostic factors were assessed by the Cox proportional hazard regression analysis. RESULTS: A total of 476 patients with stage I disease were included to analysis. Median follow-up was 68 months (4-84 months) for OS. The OS rates were related to age,CEA, CHOL, LDL-C levels,HBDH, WBC, NLR, LMR, LWR, PNI, SII, NPS and CONUT at univariate analysis. At multivariate analysis, age, WBC and SII were confirmed to be independent prognostic factors for OS. The median DFS was 68 months (2-84 months). In this period, 38 (8.0%) experienced tumor relapse, and 17 (44.7%) died due to recurrence. The DFS rates were related to higher CEA, higher NLR values and lower LMR values at univariate analysis. At multivariate analysis, just elevated CEA levels was confirmed to be independent prognostic factors. CONCLUSIONS: Patients with stage I colorectal cancers still have a clinically significant risk of recurrence. We still need to expand the number of cases to validate our findings and better identify patients who are at high risk of relapse with less severe disease.

4.
Clin Transl Med ; 11(12): e667, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34965023

RESUMO

Colorectal cancer (CRC) is one of the most common malignant tumours, with multiple driving factors and biological transitions involved in its development. Claudin-2 (CLDN2), a well-defined component of cellular tight junction, has been indicated to associate with CRC progression. However, the function of CLDN2 and the underlying mechanism whereby the downstream signalling transduction is regulated in CRC remains largely unclear. In this study, we demonstrated that CLDN2 is upregulated in CRC samples and associated with poor survival. And CLDN2 depletion significantly promotes N-myc downstream-regulated gene 1 (NDRG1) transcription, leading to termination of the CRC growth and metastasis in vitro and in vivo. Mechanistically, this process promotes CLDN2/ZO1/ZONAB complex dissociation and ZONAB shuttle into nucleus to enrich in the promoter of NDRG1. Thus, this study reveals a novel CLDN2/ZO1/ZONAB-NDRG1 axis in CRC by regulating the expression of EMT-related genes and CDKIs, suggesting CLDN2 may serve as a promising target for CRC treatment.

5.
BMC Gastroenterol ; 21(1): 446, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34823504

RESUMO

BACKGROUND: Accumulating evidence suggests a critical role of intestinal dysbacteriosis in surgical site infections and anastomotic leakage after abdominal surgery. However, a direct correlation between pre-existing dysbacteriosis and postoperative infectious complications has not yet been established clinically. METHODS: A total of 353 consecutive patients who underwent colorectal cancer (CRC) surgery were enrolled. Gram-stained faecal smears were tested at admission and the first defecation after surgery. Intestinal dysbacteriosis was graded into three groups: normal or slightly decreased intestinal microflora (grade 1), moderate dysbacteriosis (grade 2), and severe dysbacteriosis (grade 3). Clinical outcomes were postoperative infections and anastomotic leakage within 30 days after surgery. RESULTS: At the preoperative assessment, 268 (75.9%) patients had normal or slightly decreased intestinal microflora, 58 (16.4%) patients had moderate dysbacteriosis, and 27 (7.6%) patients had severe dysbacteriosis. The patients with preoperative dysbacteriosis had a higher rate of early postoperative diarrhoea (grade 2: OR = 4.53, 95% CI 2.28-9.00, grade 3: OR = 4.52, 95% CI 1.81-11.31), total complications (grade 3 40.7% vs. grade 2 25.9% vs. grade 1 12.7%, P < 0.001), and anastomotic leakage (grade 3 11.1% vs. grade 2 5.2% vs. grade 1 1.5%, P = 0.002). An interaction effect among preoperative dysbacteriosis and early postoperative diarrhoea on total complications was observed in rectal cancer patients (P for interaction = 0.007). CONCLUSIONS: An imbalance of the intestinal microbiome exists in a considerable proportion of CRC patients before surgery. Preoperative dysbacteriosis is associated with higher rates of early postoperative diarrhoea, which further correlates with infectious complications and anastomotic leakage. However, the contribution of preoperative dysbacteriosis to the occurrence of anastomotic leakage needs to be clarified in further studies. Trial registration ChiCTR, ChiCTR1800018755. Registered 8 October 2018-Retrospectively registered, http://www.chictr.org.cn/ChiCTR1800018755 .


Assuntos
Procedimentos Cirúrgicos do Sistema Digestório , Neoplasias Retais , Fístula Anastomótica/epidemiologia , Fístula Anastomótica/etiologia , Disbiose/complicações , Humanos , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Estudos Prospectivos
6.
ACS Appl Mater Interfaces ; 13(48): 57831-57840, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34807567

RESUMO

Metal-organic frameworks (MOFs) with a high surface area and excellent stability are potential candidates for uranium (U) adsorption. Amidoxime (AO) is the most widely used functional group to extract U, which is usually introduced into MOFs by two-step post-synthetic methods (PSMs). Herein, MOF UiO-66-NH-(AO) was obtained by a one-step PSM with amidoximation from UiO-66-NH-(CN), which was synthesized by a new organic ligand of 2-cyano-terephthalic acid and whose morphology was octahedron and could be well controlled with the new ligand. The one-step PSM can greatly maintain the octahedron of the MOFs. What is more, UiO-66-NH-(AO) showed good adsorption performance for U, the adsorption equilibrium was obtained within 1500 min, and the adsorption capacity of U was calculated to be 134.1 mg/g according to the Langmuir model. It also had excellent selectivity for U in the presence of high concentrations of vanadium (V), ferrum (Fe), magnesium (Mg), calcium (Ca), and zirconium (Zr). The adsorption capacity of U in natural seawater was determined to be 5.2 mg/g within 8 days. The recyclability of UiO-66-NH-(AO) in simulated seawater was demonstrated for at least four adsorption/desorption cycles. The binding mechanism was investigated by the extended X-ray absorption fine structure spectroscopy, revealing that U binding occurs in a fashion η2 motif. This study provides a reliable idea for the modification of MOFs and the potential for MOF-based materials to extract U from seawater.

7.
Br J Surg ; 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34792107

RESUMO

BACKGROUND: With local recurrence of rectal cancer continuing to decrease, distant recurrence is becoming a major concern, especially for patients with low- and intermediate-risk stage II/III rectal cancer. Therefore, a new treatment strategy is warranted for these patients. This single-arm phase II trial aimed to assess the effect of neoadjuvant chemotherapy (NCT) in low- and intermediate-risk stage II/III rectal cancer and explore candidate radiological and clinical parameters for early prediction of tumour response after two cycles of CAPOX. METHODS: Patients with mid-low stage II/III rectal cancer with low and intermediate risk were examined. The primary outcome was defined as a clinicopathological response by integrating tumour longitudinal length reduction (TLLR) on MRI into pathological tumour regression grade (TRG). After completing NCT, patients with TRG0-2 and TRG3 with a TLLR rate greater than 30 per cent were considered to be responders. Secondary outcomes included pathological complete response (pCR), adverse events and local and distant recurrence. RESULTS: This study enrolled 61 eligible patients. No patient was converted to neoadjuvant chemoradiotherapy owing to tumour progression. The clinicopathological response and pCR rates were 78.7 and 21.3 per cent respectively. After two cycles of CAPOX, TLLR, TRG on MRI, and mucosal lesion regression grade on endoscopy had potential discriminative ability (area under the curve greater than 0.7) for predicting both clinicopathological and pathological response. CONCLUSION: NCT alone achieves good tumour response rates in patients with low- and intermediate-risk stage II/III rectal cancer, and predicting tumour response to NCT is feasible at an early treatment phase. REGISTRATION NUMBER: NCT03666442 (http://www.clinicaltrials.gov).

9.
Cell Biosci ; 11(1): 192, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34758883

RESUMO

BACKGROUND: Aging and neurodegenerative diseases are typical metabolic-related processes. As a metabolism-related long non-coding RNA, EPB41L4A-AS has been reported to be potentially involved in the development of brain aging and neurodegenerative diseases. In this study, we sought to reveal the mechanisms of EPB41L4A-AS in aging and neurodegenerative diseases. METHODS: Human hippocampal gene expression profiles downloaded from the Genotype-Tissue Expression database were analyzed to obtain age-stratified differentially expressed genes; a weighted correlation network analysis algorithm was then used to construct a gene co-expression network of these differentially expressed genes to obtain gene clustering modules. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, protein-protein interaction network, and correlation analysis were used to reveal the role of EPB41L4A-AS1. The mechanism was verified using Gene Expression Omnibus dataset GSE5281 and biological experiments (construction of cell lines, Real-time quantitative PCR, Western blot, measurement of ATP and NAD+ levels, nicotinamide riboside treatment, Chromatin Immunoprecipitation) in neurons and glial-derived cells. RESULTS: EPB41L4A-AS1 was downregulated in aging and Alzheimer's disease. EPB41L4A-AS1 related genes were found to be enriched in the electron transport chain and NAD+ synthesis pathway. Furthermore, these genes were highly associated with neurodegenerative diseases and positively correlated with EPB41L4A-AS1. In addition, biological experiments proved that the downregulation of EPB41L4A-AS1 could reduce the expression of these genes via histone H3 lysine 27 acetylation, resulting in decreased NAD+ and ATP levels, while EPB41L4A-AS1 overexpression and nicotinamide riboside treatment could restore the NAD+ and ATP levels. CONCLUSIONS: Downregulation of EPB41L4A-AS1 not only disturbs NAD+ biosynthesis but also affects ATP synthesis. As a result, the high demand for NAD+ and ATP in the brain cannot be met, promoting the development of brain aging and neurodegenerative diseases. However, overexpression of EPB41L4A-AS1 and nicotinamide riboside, a substrate of NAD+ synthesis, can reduce EPB41L4A-AS1 downregulation-mediated decrease of NAD+ and ATP synthesis. Our results provide new perspectives on the mechanisms underlying brain aging and neurodegenerative diseases.

10.
ACS Omega ; 6(44): 29675-29684, 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34778639

RESUMO

Owing to many problems of the detriment by large amount of organic reagents, high cost and difficulty of industrialization, development of high-efficiency economical technologies for uranium extraction is an irresistible trend to support steady supply of nuclear energy. Herein, a novel fibrous adsorbent, named as AO-HPE fibers, was prepared by introduction of amidoxime groups using the green vapor-phase grafting polymerization (VPGP) technology of monomer acrylonitrile (AN). Gaseous AN was grafted onto the ultra high molecular weight polyethylene (UHMWPE) fibers at 80 °C in the enclosed evaporation and condensation reflux system. The innovative technology not only endowed synthetic process high monomer utilization ratio but also excellent environmental friendliness. The AO-HPE fibers exhibited an appreciable calculated maximum adsorption capacity (Q m) of 1144.94 mg·g-1 in uranium solution and an adsorption capacity of 14.11 mg·g-1 in simulated seawater. Meanwhile, the higher uranium selectivity than main competing ion vanadium (adsorption mass ratio was almost 5) was achieved. The adsorption process accorded closely with chemisorption mechanism. This work provided a novel idea for the synthetic method of adsorbents for uranium extraction, and inspired the sustainable technologies for grafting polymerization of monomer AN.

11.
ACS Appl Mater Interfaces ; 13(40): 47458-47464, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34605239

RESUMO

The design of Au-based materials with good dispersion and more active sites is critical to enhance the catalytic performance of electrochemical ammonia production. Herein, two-dimensional (2D) heterojunction Au-Bi2Te3 nanosheets (Au-Bi2Te3 NSs) are prepared by Au nanoparticles growing on Bi2Te3 nanosheets. Benefiting from the good dispersion of Au nanoparticles and the synergistic effect of the heterojunction composite, Au-Bi2Te3 NSs demonstrate excellent behavior for an ambient nitrogen reduction reaction (NRR). In a 0.1 M Na2SO4 electrolyte (N2-saturated), Au-Bi2Te3 NSs display a high NH3 yield rate of 32.73 µg h-1 mgcat.-1 and a faradic efficiency of 20.39% at -0.4 V. The proposed synthetic method provides a feasible strategy for designing high-performance heterojunction electrocatalysts for electrochemical ammonia synthesis.

12.
Front Mol Biosci ; 8: 703640, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595207

RESUMO

Exosomes or other extracellular vesicles released from cells play an important role in cell-to-cell communication by transferring bio-information (DNA, coding/non-coding RNA, and proteins), which indicates parental cell status to recipient cells in the extracellular environment. Increasingly, evidence shows that tumor-derived exosomes mediate tumor pre-metastatic niche (PMN) remodeling to establish a supportive and receptive niche to promote tumor cell colonization and metastasis. Uptake of genetic information by target cells in the extracellular environment triggers epigenetic changes that contribute to PMN formation. Here, we provide a comprehensive overview of the current understanding of exosomes-mediated reprogramming of cells in PMN formation.

13.
ANZ J Surg ; 91(11): 2487-2492, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34664380

RESUMO

BACKGROUND: This study aimed to evaluate the perioperative, pathological and oncological outcomes of open versus laparoscopic total mesorectal excision (TME) plus lateral lymph node dissection (LLND) for mid- and low- rectal cancer using propensity score matching. METHODS: Between September 2009 and December 2018, consecutive patients who underwent open or laparoscopic TME plus LLND were collected. Based on the propensity score matching analysis, laparoscopic TME plus LLND was compared with open TME plus LLND for the perioperative, pathological, and oncological outcomes. RESULTS: A total of 77 patients were collected. There were 13 patients who underwent open TME plus LLND (open group) and 64 patients who underwent laparoscopic TME plus LLND (laparoscopic group). After propensity score matching, 13 patients in the open group were matched to 39 patients in the laparoscopic group (1:3). The laparoscopic group had a significantly shorter length of incision (5 cm vs. 20 cm, p < 0.01), less intraoperative blood loss (80 mL vs. 100 mL, p = 0.02), and shorter time to postoperative liquid diet (72 h vs. 96 h, p = 0.05). There were no significant differences in both early and late postoperative complications. The 3-year overall survival was 85.9% in the laparoscopic group and 76.9% in the open group (p = 0.75), respectively. The 3-year disease-free survival was 70.6% in the laparoscopic group and 74.1% in the open group (p = 0.83), respectively. CONCLUSION: Laparoscopic LLND had comparable oncological outcomes to open LLND. Moreover, laparoscopic LLND had postoperative recovery advantages over open LLND.


Assuntos
Laparoscopia , Neoplasias Retais , Humanos , Excisão de Linfonodo , Linfonodos/cirurgia , Pontuação de Propensão , Neoplasias Retais/cirurgia , Estudos Retrospectivos , Resultado do Tratamento
14.
Commun Biol ; 4(1): 1207, 2021 10 21.
Artigo em Inglês | MEDLINE | ID: mdl-34675360

RESUMO

Numbers of nuclear speckles and paraspeckles components have been demonstrated to regulate herpes simplex virus 1 (HSV-1) replication. However, how HSV-1 infection affects the two nuclear bodies, and whether this influence facilitates the expression of viral genes, remains elusive. In the current study, we found that HSV-1 infection leads to a redistribution of speckles and paraspeckles components. Serine/arginine-rich splicing factor 2 (SRSF2), the core component of speckles, was associated with multiple paraspeckles components, including nuclear paraspeckles assembly transcript 1 (NEAT1), PSPC1, and P54nrb, in HSV-1 infected cells. This association coordinates the transcription of viral genes by binding to the promoters of these genes. By association with the enhancer of zeste homolog 2 (EZH2) and P300/CBP complex, NEAT1 and SRSF2 influenced the histone modifications located near viral genes. This study elucidates the interplay between speckles and paraspeckles following HSV-1 infection and provides insight into the mechanisms by which HSV-1 utilizes host cellular nuclear bodies to facilitate its life cycle.

15.
ACS Appl Mater Interfaces ; 13(37): 44733-44741, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34499470

RESUMO

Achieving high efficiency in nitrate (NO3-) to ammonia (NH3) electrocatalysis requires the exploration of advanced electrocatalysts with a well-designed composition and architecture. In this work, a facile one-step hydrothermal approach was developed for the construction of novel Cu/oxygen vacancy-rich Cu-Mn3O4 heterostructured ultrathin nanosheet arrays on Cu foam (Cu/Cu-Mn3O4 NSAs/CF). Two-dimensional ultrathin nanosheet arrays could increase the exposure of catalytically active centers, and the heterogeneous nanointerface and oxygen vacancies synergistically improve the nitrate-to-ammonia activity over the active centers. Due to the desirable compositional and structural advantages, the Cu/Cu-Mn3O4 NSAs/CF demonstrated excellent performance for the electrocatalytic nitrate reduction to ammonia with high ammonia selectivity (87.6%) and Faradic efficiency (92.4%).

16.
Nature ; 599(7884): 222-228, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34587621

RESUMO

The transition metal kagome lattice materials host frustrated, correlated and topological quantum states of matter1-9. Recently, a new family of vanadium-based kagome metals, AV3Sb5 (A = K, Rb or Cs), with topological band structures has been discovered10,11. These layered compounds are nonmagnetic and undergo charge density wave transitions before developing superconductivity at low temperatures11-19. Here we report the observation of unconventional superconductivity and a pair density wave (PDW) in CsV3Sb5 using scanning tunnelling microscope/spectroscopy and Josephson scanning tunnelling spectroscopy. We find that CsV3Sb5 exhibits a V-shaped pairing gap Δ ~ 0.5 meV and is a strong-coupling superconductor (2Δ/kBTc ~ 5) that coexists with 4a0 unidirectional and 2a0 × 2a0 charge order. Remarkably, we discover a 3Q PDW accompanied by bidirectional 4a0/3 spatial modulations of the superconducting gap, coherence peak and gap depth in the tunnelling conductance. We term this novel quantum state a roton PDW associated with an underlying vortex-antivortex lattice that can account for the observed conductance modulations. Probing the electronic states in the vortex halo in an applied magnetic field, in strong field that suppresses superconductivity and in zero field above Tc, reveals that the PDW is a primary state responsible for an emergent pseudogap and intertwined electronic order. Our findings show striking analogies and distinctions to the phenomenology of high-Tc cuprate superconductors, and provide groundwork for understanding the microscopic origin of correlated electronic states and superconductivity in vanadium-based kagome metals.

17.
Nature ; 599(7884): 216-221, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34587622

RESUMO

The kagome lattice of transition metal atoms provides an exciting platform to study electronic correlations in the presence of geometric frustration and nontrivial band topology1-18, which continues to bear surprises. Here, using spectroscopic imaging scanning tunnelling microscopy, we discover a temperature-dependent cascade of different symmetry-broken electronic states in a new kagome superconductor, CsV3Sb5. We reveal, at a temperature far above the superconducting transition temperature Tc ~ 2.5 K, a tri-directional charge order with a 2a0 period that breaks the translation symmetry of the lattice. As the system is cooled down towards Tc, we observe a prominent V-shaped spectral gap opening at the Fermi level and an additional breaking of the six-fold rotational symmetry, which persists through the superconducting transition. This rotational symmetry breaking is observed as the emergence of an additional 4a0 unidirectional charge order and strongly anisotropic scattering in differential conductance maps. The latter can be directly attributed to the orbital-selective renormalization of the vanadium kagome bands. Our experiments reveal a complex landscape of electronic states that can coexist on a kagome lattice, and highlight intriguing parallels to high-Tc superconductors and twisted bilayer graphene.

18.
Int J Biol Sci ; 17(13): 3428-3440, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34512157

RESUMO

The nuclear paraspeckle assembly transcript 1 (NEAT1) is a long non-coding RNA (lncRNA) that is upregulated in a variety of human cancer types. Increasing evidence has shown that the elevation of NEAT1 in cancer cells promotes cell growth, migration, and invasion and inhibits cell apoptosis. It is also known that lncRNAs act as a competing endogenous RNA (ceRNA) by sponging microRNAs (miRNAs) to alter the expression levels of their target genes in the development of cancers. Therefore, it is important to understand the molecular mechanisms underlying this observation. In this review, specific emphasis was placed on NEAT1's role in tumor development. We also summarize and discuss the feedback roles of NEAT1/miRNA/target network in the progression of various cancers. As our understanding of the role of NEAT1 during tumorigenesis improves, its therapeutic potential as a biomarker and/or target for cancer also becomes clearer.

19.
Nanoscale ; 13(32): 13809-13815, 2021 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-34477655

RESUMO

Electrochemical reduction of nitrogen to ammonia has received considerable attention for sustainable nitrogen fixation, but the sluggish kinetics results in unsatisfactory activity and efficiency. Designing electrocatalytic active centers for nitrogen adsorption and activation is highly desired. Herein, we present an electrodeposition method for the synthesis of a phosphorus-doped mesoporous rhodium film on nickel foam for the electrochemical synthesis of ammonia. Due to the unique combination of components and structure, the obtained catalyst not only shows excellent catalytic performance (NH3 yield: 32.57 µg h-1 mg-1cat.; faradaic efficiency: 40.86%), but also exhibits almost no decrease in activity after the durability test. This research work can provide a facile synthesis strategy for non-metal-doped porous metal based catalysts, which would be promising for the electrochemical synthesis of ammonia.

20.
ACS Omega ; 6(36): 23243-23261, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34549125

RESUMO

The pore structure and movable fluid characteristics of tight conglomerate reservoirs are complex, which are greatly different from conventional reservoirs. The depositional mechanism is the fundamental factor controlling the physical properties of conglomerate reservoirs. However, there is a lack of systematic research on the pore structure and movable fluid characteristics of conglomerate reservoirs with typical sedimentary facies. This paper investigates the pore structure and movable fluid characteristics of conglomerate of different sedimentary facies based on various experiments. Casting thin sections, X-ray diffraction, scanning electron microscopy, high-pressure mercury injection, and nuclear magnetic resonance experiments were conducted on 32 conglomerates samples from the Mahu Sag, Junggar Basin, China. The quality classification method of tight conglomerate reservoirs is established. The results show that the conglomerate can be divided into three sedimentary facies; traction flow conglomerate (TFC) and pebbled sandstone (PSS) mainly develop intergranular pores and dissolved pores; and the pore diameter curves are mainly a double peak, single peak, and flat peak. Gravity flow conglomerate (GFC) mainly develops dissolved pores and interstitial micropores, and the pore diameter curve is mainly a single peak. PSS includes pebbled gritty sandstone (P(G)SS) and pebbled fine sandstone (P(F)SS). TFC and P(G)SS are favorable class I reservoirs, while GFC and P(F)SS are nonfavorable class II reservoirs. A new parameter, the ratio of the major axis to the minor axis of the pore outer ellipse (axial ratio), is proposed to quantitatively describe the compaction effect. The average axial ratios of the three lithofacies are 3.04, 3.98, and 8.78, respectively, indicating that the compaction is intensified and the pore structure becomes worse. By analyzing the correlation between pore structure parameters and permeability, it is found that the main controlling factors of permeability of GFC and TFC are sorting and connectivity, respectively, and the main flow radius is the most suitable parameter to describe permeability. A linear spectral decomposition method was used to establish a new quantitative calculation method of movable fluid saturation for different types of pores, and the results show that the movable fluid saturation of intergranular pores is the highest (average: 65.43%), and the movable fluid saturation of TFC and P(G)SS with more intergranular pores is the highest. Movable fluid saturation is inversely proportional to the content of I/S and the compaction rate and positively proportional to the content of quartz and feldspar and the cementation rate. The fluid mobility of water-wet samples is weaker. The research results provide theoretical support for the identification of favorable reservoirs and the cognition of a development mechanism.

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