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1.
Artigo em Inglês | MEDLINE | ID: mdl-31683491

RESUMO

BACKGROUND: Dry-electrode-based transcranial direct current stimulation is a new type of non-invasive brain stimulation system which relieves chronic low back pain and improves related muscle movement, in a way that overcomes the drawback of conventional systems. OBJECTIVE: To investigate the effectiveness of dry-electrode-based transcranial direct current stimulation in relieving chronic low back pain and altering pain-related low back muscles movement, by using pain assessment tool and surface electromyographic topography. METHODS: We conducted a prospective, double-blind, randomized, sham-controlled study. 60 patients with non-specific chronic low back pain were randomly and evenly allocated into tDCS and sham groups. Each group accepted a single 20-minute stimulation at 2 mA on the primary motor cortex. Numeric rating scale for pain intensity assessment and root-mean-square difference parameter from surface electromyographic topography were measured before and after stimulation. The current direction in brain using finite element method was simulated to verify the current distribution under dry stimulation electrode. RESULTS: After stimulation, the pain intensity in the tDCS group significantly decreased, while it did not show evident change in the sham group. However, change of root-mean-square difference parameters between tDCS and sham groups showed no significant difference. Simulation results based on finite element method showed most of current focused on primary motor cortex while peak value of current density was 0.225 A/m2. CONCLUSIONS: Dry-electrode-based transcranial direct current stimulation can lower pain perception in patients with chronic low back pain. The analgesic mechanism can affect the top-down modulation pathway of pain.

2.
Artif Cells Nanomed Biotechnol ; 47(1): 4211-4221, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31713444

RESUMO

At present, cancer is the first cause of death for humans, but early detection and treatment can help improve prognoses and reduce mortality. However, further development of carrier-assistant drug delivery systems (DDSs) is retarded by the aspects such as the low drug-carrying capacity, carrier-induced toxicity and immunogenicity, complex synthesis manipulation. The development of nanoscale drug delivery systems (NDDS) have been rapidly developed to address these issues. In this article, we used PLGA-PEG with good biocompatibility to encapsulate Fe3O4 nanoparticles (a magnetic resonance imaging contrast agent) and DOX (an antitumour drug) via the emulsion-solvent evaporation method, aimed at achieving a dual function of the early detection and the treatment of mammary cancer. The results showed that the Fe3O4/DOX/PLGA-PEG nanoparticles had a relatively uniform size, a high carrier rate of Fe3O4 and high encapsulation efficiency of DOX, and a relatively high activity of released DOX within 120 h. In addition, in vitro studies showed that the Fe3O4/DOX/PLGA-PEG nanoparticles were cytocompatibility in NIH 3T3 fibroblast cells culture study while had a special effect on destroying human breast cancer MCF-7 cells compared with pure DOX solution. In vitro studies revealed that the Fe3O4/DOX/PLGA-PEG enabled enhanced T2 contrast magnetic resonance. Overall, our multifunctional Fe3O4/DOX/PLGA-PEG nanoparticles, composed of biocompatible substances and therapeutic/imaging materials, have great potential for the early detection of cancer and accurate drug delivery via the dynamic monitoring using MRI.

3.
Zhongguo Zhen Jiu ; 39(11): 1239-43, 2019 Nov 12.
Artigo em Chinês | MEDLINE | ID: mdl-31724364

RESUMO

Under the revised demand of theoretic development in clinical practice and scientific research of acupuncture and moxibustion, the reconstruction of the theory was discussed on the "three yin and three yang" subarea division in the body and acupuncture effect in line with the traditional thinking of Chinese medicine so as to explore the longitudinal foundation and rule in the human body for the "effective stimulation". ①Based on the theory of "qi-space correlation" in Internal Classic and the source of nomenclature of "three yin and three yang", the body is divided in stereo zone in accordance with the theory of "three yin and three yang". ②In view of theoretical reasoning and practical interpretation, it is expounded that the same longitudinal stereo zone subarea in the body could respond to diseases and generate therapeutic effects. ③It is pointed out that the subareas of "three yin and three yang" in the body are closely related to meridians and collaterals, which is the basis of the spatial structure and and physical effect. The importance of the "body" itself is ignored nowadays because of the conventional thinking of "qi and blood" in traditional Chinese medicine.


Assuntos
Terapia por Acupuntura , Acupuntura , Meridianos , Corpo Humano , Humanos , Medicina Tradicional Chinesa , Yin-Yang
4.
IEEE Trans Cybern ; 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31581106

RESUMO

In this article, we are concerned with the synchronization problem of Kuramoto oscillators under the sampled-data updating law. This article is motivated by the needs of synchronization of Kuramoto oscillators in the presence of periodic and asynchronous coupling updates. Based on the periodical sampled-data method, a sufficient condition ensuring synchronization under periodic updates is derived. In order to relax the requirement of having all data updated simultaneously, an event-triggered law is designed to implement asynchronous coupling updates. Our synchronization analysis does not rely on any linearization technique around equilibrium points. Instead, we employ the Lyapunov stability theory and nonsmooth analysis technique to deduce the synchronization conditions and estimate the region of attraction. The effectiveness of the proposed sampled-data coupling is illustrated by numerical simulations.

5.
Phys Chem Chem Phys ; 21(40): 22629-22638, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31591617

RESUMO

Solid solution strengthening has been widely used in designing various high-performance biocompatible Mg-based alloys, but its transferability to other biocompatible metals such as Zn-based alloys is questionable or nearly absent. In the present study, an ab initio informed Peierls-Nabarro model and Leyson et al.'s strengthening model are used for a systematic investigation on solute strengthening in Zn-based alloys, which is compared with the widely studied Mg-based alloys. Although an inverse relationship was revealed between volume misfit εb and chemical misfit εSFE for both Zn-based and Mg-based alloys, most solutes would however result in positive εb and negative εSFE for Zn-based alloys, differing from Mg-based alloys. With εb and εSFE as two key descriptors, a generalized scaling diagram is finally drawn for a fast evaluation of solid solution strengthening in Zn-based alloys, indicating that the alkaline-earth and rare earth elements are better strengtheners for Zn-based alloys, which provides a general rule in designing novel biocompatible materials.

6.
World J Gastroenterol ; 25(38): 5838-5849, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31636476

RESUMO

BACKGROUND: Prolonged postoperative ileus (PPOI) is one of the common complications in gastric cancer patients who underwent gastrectomy. Evidence on the predictors of PPOI after gastrectomy is limited and few prediction models of nomogram are used to estimate the risk of PPOI. We hypothesized that a predictive nomogram can be used for clinical risk estimation of PPOI in gastric cancer patients. AIM: To investigate the risk factors for PPOI and establish a nomogram for clinical risk estimation. METHODS: Between June 2016 and March 2017, the data of 162 patients with gastrectomy were obtained from a prospective and observational registry database. Clinical data of patients who fulfilled the criteria were obtained. Univariate and multivariable logistic regression models were performed to detect the relationship between variables and PPOI. A nomogram for PPOI was developed and verified by bootstrap resampling. The calibration curve was employed to detect the concentricity between the model probability curve and ideal curve. The clinical usefulness of our model was evaluated using the net benefit curve. RESULTS: This study analyzed 14 potential variables of PPOI in 162 gastric cancer patients who underwent gastrectomy. The incidence of PPOI was 19.75% in patients with gastrectomy. Age older than 60 years, open surgery, advanced stage (III-IV), and postoperative use of opioid analgesic were independent risk factors for PPOI. We developed a simple and easy-to-use prediction nomogram of PPOI after gastrectomy. This nomogram had an excellent diagnostic performance [area under the curve (AUC) = 0.836, sensitivity = 84.4%, and specificity = 75.4%]. This nomogram was further validated by bootstrapping for 500 repetitions. The AUC of the bootstrap model was 0.832 (95%CI: 0.741-0.924). This model showed a good fitting and calibration and positive net benefits in decision curve analysis. CONCLUSION: We have developed a prediction nomogram of PPOI for gastric cancer. This novel nomogram might serve as an essential early warning sign of PPOI in gastric cancer patients.

7.
Proc Natl Acad Sci U S A ; 116(42): 21256-21261, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31578252

RESUMO

Hydrogen peroxide (H2O2) is an important messenger molecule for diverse cellular processes. H2O2 oxidizes proteinaceous cysteinyl thiols to sulfenic acid, also known as S-sulfenylation, thereby affecting the protein conformation and functionality. Although many proteins have been identified as S-sulfenylation targets in plants, site-specific mapping and quantification remain largely unexplored. By means of a peptide-centric chemoproteomics approach, we mapped 1,537 S-sulfenylated sites on more than 1,000 proteins in Arabidopsis thaliana cells. Proteins involved in RNA homeostasis and metabolism were identified as hotspots for S-sulfenylation. Moreover, S-sulfenylation frequently occurred on cysteines located at catalytic sites of enzymes or on cysteines involved in metal binding, hinting at a direct mode of action for redox regulation. Comparison of human and Arabidopsis S-sulfenylation datasets provided 155 conserved S-sulfenylated cysteines, including Cys181 of the Arabidopsis MITOGEN-ACTIVATED PROTEIN KINASE4 (AtMAPK4) that corresponds to Cys161 in the human MAPK1, which has been identified previously as being S-sulfenylated. We show that, by replacing Cys181 of recombinant AtMAPK4 by a redox-insensitive serine residue, the kinase activity decreased, indicating the importance of this noncatalytic cysteine for the kinase mechanism. Altogether, we quantitatively mapped the S-sulfenylated cysteines in Arabidopsis cells under H2O2 stress and thereby generated a comprehensive view on the S-sulfenylation landscape that will facilitate downstream plant redox studies.

8.
Eur J Med Chem ; 184: 111779, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31629165

RESUMO

The burst of reactive oxygen species (ROS) contributes to and exacerbates cardiac injury. Exogenous supplementation of antioxidants or upregulation of endogenous antioxidant defense genes should be the potential therapies for cardiovascular disease. Sixteen coumarin-derived imino sulfonates compounds were synthesized with the ability of attenuating oxidative stress directly by reducing intracellular ROS level via promoting Nrf2 pathway. The cell-based assays showed that most of the compounds had significant protective activity against H2O2-induced oxidative injury in H9c2 cells. Compound 5h with the highest activity and low cytotoxicity was demonstrated to remarkably remove the intracellular ROS accumulation by activating expressions of Nrf2 and its downstream antioxidant proteins (ie. HO-1 and NQO1), indicating a novel promising antioxidant and Nrf2 activator. Overall, these findings demonstrated that compound 5h could serve as a potential cardioprotective agent. Moreover, our study features developing new antioxidants and Nrf2 activators by introducing a sulfonyl group and nitrogen atom to the α,ß-unsaturated carbonyl entity in coumarin, rather than adding new functional groups or active fragments to coumarin itself.

9.
Mol Med Rep ; 20(6): 5111-5117, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31638198

RESUMO

Usher syndrome is the most common condition of combined blindness and deafness and is classified into three types (USH1­USH3). USH2 is the most commonly diagnosed of all Usher syndrome cases. There are three identified proteins (usherin, GPR98 and whirlin) that form the USH2 complex. Defects in any of these proteins may cause failure in the formation of the USH2 complex, which is the primary cause of USH2. Whirlin is a scaffold protein and is essential for the assembly of the USH2 protein complex. It has been reported that espin is an interacting partner protein for whirlin. However, which fragment of whirlin interacts with espin remains unclear. In the present study, whirlin N­ and C­terminal fragments in the pEGFP­C2 vectors were constructed. The recombinant plasmids were transfected into COS­7 cells to observe the co­localization by confocal laser scanning microscopy. The interactions between whirlin and espin were investigated by co­immunoprecipitation using the 293 cell line. It was demonstated that only the whirlin N­terminal fragment was able to interact with espin and the PR (proline­rich) region in whirlin may be important for the interaction. However, the present study did not investigate the interaction between whirlin and espin without the PR domain which warrants future research. Our findings elucidated a primary mechanism of interaction between whirlin and espin, which are crucial for further study on the USH2 complex and USH2 pathogenesis.

10.
PLoS One ; 14(10): e0223825, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31639168

RESUMO

To investigate morphological characteristics and generation mechanism of the machined gears surface, image characteristics of machined surface morphology including profile roughness, fractal and textural characteristics were studied. the change of profile curves for the surface image is subject to the normal probability density function and the W-M function. The orientation angle of surface texture is 0°, the surface profile curves are the smoothest and have the most uniform, regular textures. When the texture orientation is 45° or 135°, the surface profile curves show large fluctuations, while surface image textures present the deepest grooves and are shown to be distributed most irregularly. Additionally, the influence mechanism of different grinding parameters on the morphological characteristics of machined surface was investigated. The quality of machined surfaces increased with the grinding speed while deteriorated with increasing radial, or axial, feed speeds.

11.
Med Sci Monit ; 25: 7342-7350, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31566195

RESUMO

BACKGROUND This study aimed to evaluate an autologous bone mesenchymal stem cell (MSC)-derived extracellular matrix (ECM) scaffold in two animal models of cartilage repair. MATERIAL AND METHODS A rabbit model (n=16) and a minipig model (n=8) of cartilage repair were created with cartilage defects of the knee joints treated with bone marrow stimulation (BMS). In the ECM group, autologous bone MSC-derived ECM scaffolds were implanted into the cartilage defects after bone marrow stimulation. In the BMS group, the cartilage defects were treated by bone marrow stimulation only. The renewal capacity of bone MSCs was measured with a colony-forming unit fibroblast (CFU-F) in vitro assay. The extent of cartilage repair was as-sessed at 6 months after surgery. RESULTS In the rabbit model, the macroscopic appearance of the exudate of the healing wounds in the ECM group showed less fibrosis, and the histology showed more evenly distributed chondrocytes compared with the BMS group. The CFU-F assay showed that the number of bone MSCs in the ECM group was approximately was twice that of the BMS group. In the minipig model, the macroscopic appearance and magnetic resonance imaging (MRI) findings of the ECM group were improved when compared with the BMS group. The repaired tissue in ECM group had similar histological characteristics and biochemical content to normal hyaline cartilage. CONCLUSIONS In two animal models of knee joint cartilage repair, the use of an ECM scaffold increased the number of bone MSCs and improved the extent of cartilage repair.

12.
Sensors (Basel) ; 19(19)2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31575038

RESUMO

This paper designs an accurate and low-cost phishing detection sensor by exploring deep learning techniques. Phishing is a very common social engineering technique. The attackers try to deceive online users by mimicking a uniform resource locator (URL) and a webpage. Traditionally, phishing detection is largely based on manual reports from users. Machine learning techniques have recently been introduced for phishing detection. With the recent rapid development of deep learning techniques, many deep-learning-based recognition methods have also been explored to improve classification performance. This paper proposes a light-weight deep learning algorithm to detect the malicious URLs and enable a real-time and energy-saving phishing detection sensor. Experimental tests and comparisons have been conducted to verify the efficacy of the proposed method. According to the experiments, the true detection rate has been improved. This paper has also verified that the proposed method can run in an energy-saving embedded single board computer in real-time.

13.
IEEE Trans Cybern ; 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31613789

RESUMO

There are two common challenges in particle swarm optimization (PSO) research, that is, selecting proper exemplars and designing an efficient learning model for a particle. In this article, we propose a triple archives PSO (TAPSO), in which particles in three archives are used to deal with the above two challenges. First, particles who have better fitness (i.e., elites) are recorded in one archive while other particles who offer faster progress, called profiteers in this article, are saved in another archive. Second, when breeding each dimension of a potential exemplar for a particle, we choose a pair of elite and profiteer from corresponding archives as two parents to generate the dimension value by ordinary genetic operators. Third, each particle carries out a specific learning model according to the fitness of its potential exemplars. Furthermore, there is no acceleration coefficient in TAPSO aiming to simplify the learning models. Finally, if an exemplar has excellent performance, it will be regarded as an outstanding exemplar and saved in the third archive, which can be reused by inferior particles aiming to enhance the exploitation and to save computing resources. The experimental results and comparisons between TAPSO and other eight PSOs on 30 benchmark functions and four real applications suggest that TAPSO attains very promising performance in different types of functions, contributing to both higher solution accuracy and faster convergence speed. Furthermore, the effectiveness and efficiency of these new proposed strategies are discussed based on extensive experiments.

14.
IEEE Trans Cybern ; 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31634858

RESUMO

In this article, we consider the distributed synchronization problem of dynamic networked systems with adaptive nonlinear couplings. Based on how the information is collected, the interactions between subsystems are characterized by nonlinear relative state couplings and nonlinear absolute state couplings. In both cases, we show that the considered nonlinear interactions can be used to simulate the couplings with disturbed relative or absolute states. In order to implement the nonlinear couplings in a fully distributed fashion, adaptive control laws are proposed for the adjustment of coupling strengths between connected subsystems. It is shown that the connected network topology is sufficient to ensure the synchronization of dynamic networked systems with the proposed adaptive nonlinear coupling methods. Different from many existing works, the σ-modification technique is used to suppress the increase of the coupling strengths with an additional benefit of preventing the coupling strengths from increasing. Simulation examples are given to assess the performance of the proposed adaptive nonlinear couplings.

15.
Pharmacol Res ; 148: 104445, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31526872

RESUMO

Atherosclerosis is one of the leading causes of morbidity and mortality worldwide. A significant increase in ganglioside GM3 content generally happens in atherosclerotic plaques causing a GM3-enriched microenvironment. It remains unclear whether the GM3-enriched microenvironment influences atherogenesis. This study sought to answer the question by investigating exogenous GM3 effects on multiple steps involved in atherogenesis. First, the physicochemical properties of native low-density lipoprotein (LDL) and LDL enriched with exogenous GM3 (GM3-LDL) were characterized by dynamic laser scattering, atomic force microscopy, and agarose gel electrophoresis. Then, electrophoretic mobility, conjugated diene and malondialdehyde production, and amino group blockage of GM3-LDL/LDL were measured to determine LDL oxidation degrees and cellular recognition/internalization of GM3-LDL/GM3-oxLDL were detected via confocal microscopy and flow cytometry. Subsequently, influences of exogenous GM3 addition on the monocyte-adhering ability of endothelial cells and on lipid deposition in macrophages were investigated. Finally, exogenous GM3 effect on atherogenesis was evaluated using apoE-/- mice fed a high-fat diet. We found that exogenous GM3 addition increased the size, charge, and stability of LDL particles, reduced LDL susceptibility to oxidation and its cellular recognition/internalization, impaired the monocyte-adhering ability of endothelial cells and lipid deposition in macrophages. Moreover, exogenous GM3 treatment also significantly decreased blood lipid levels and atherosclerotic lesion areas in atherosclerotic mice. The data imply that exogenous GM3 had an inhibitory effect on atherogenesis, suggesting a protective role of a GM3-enriched microenvironment in atherosclerotic plaques and implying a possibility of exogenous GM3 as an anti-atherosclerotic drug.

16.
World J Gastroenterol ; 25(31): 4452-4467, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31496624

RESUMO

BACKGROUND: Adenoma polyposis coli (APC) mutation is associated with tumorigenesis via the Wnt signaling pathway. AIM: To investigate the clinical features and mechanism of APC expression in gastric cancer (GC). METHODS: Based on APC expression profile, the related genome-wide mRNA expression, microRNA (miRNA) expression, and methylation profile in GC, the relationship between APC and GC, as well as the prognostic significance of APC were systematically analyzed by multi-dimensional methods. RESULTS: We found that high expression of APC (APC high) was significantly associated with adverse outcomes of T4 GC patients. Genome-wide gene expression analysis revealed that varying APC expression levels in GC were associated with some important oncogenes, and corresponding cellular functional pathways. Genome-wide miRNA expression analysis indicated that most of miRNAs associated with high APC expression were downregulated. The mRNA-miRNA regulatory network analysis revealed that down-regulated miRNAs affected their inhibitory effect on tumor genes. Genome-wide methylation profiles associated with APC expression showed that there was differential methylation between the APC high and APC low groups. The number of hypermethylation sites was larger than that of hypomethylation sites, and most of hypermethylation sites were enriched in CpG islands. CONCLUSION: Our research demonstrated that high APC expression is an unfavorable prognostic factor for T4 GC patients and may be used as a novel biomarker for pathogenesis research, diagnosis, and treatment of GC.

17.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 41(4): 464-471, 2019 Aug 30.
Artigo em Chinês | MEDLINE | ID: mdl-31484607

RESUMO

Objective To evaluate the early and midterm results of surgical repair of thoracoabdominal aortic aneurysm(TAAA)in patients with Marfan syndrome(MFS). Methods The clinical data of patients with MFS undergoing TAAA repair in Fuwai Hospital between January 2009 and December 2017 were retrospectively analyzed.These patients were divided into two groups:MFS group(n=58)and non-MFS group(n=98).The baseline data,early postoperative results,and midterm follow-up outcomes were compared between these two groups. Results MFS patients were significantly younger(32 years old vs. 45 years old,t=9.603,P=0.000)and more frequently had a history of aortic aneurysm or dissection(19% vs. 0,χ 2=19.996,P=0.000)than non-MFS patients.However,the proportions of males and smokers were significantly lower when compared with non-MFS patients(55.2% vs. 80.6%,χ 2=11.489,P=0.001;13.8% vs. 46.9%,χ 2=17.686,P=0.001).There was no significant difference in proportion of emergency operation,prophylactic cerebrospinal fluid drainage,operation time,intra-operative circulation management,and intra-operative blood transfusion(all P>0.05).The 30-day mortality rate was significantly lower in MFS group than in non-MFS group(0 vs. 9.2%, [Formula: see text]=5.034,P=0.025). Conclusions For patients with MFS,TAAA repair provides lower 30-day mortality and comparative middle-term survival.However,the re-intervention rate is higher among MFS patients,highlighting the importance of close follow-up.


Assuntos
Aneurisma da Aorta Torácica/cirurgia , Implante de Prótese Vascular , Síndrome de Marfan/complicações , Adulto , Aneurisma Dissecante , Aneurisma da Aorta Torácica/complicações , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
18.
Pharmacol Res ; : 104451, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31557524

RESUMO

Hypertension ranks the most common risk factor for cardiovascular diseases, and it affects almost one third of adult population globally. Emerging evidence indicates that immune activation is highly involved in the entire progress of hypertension and end organ damage. In addition to immunity, autonomic nervous system, particularly sympathetic nervous system, is one of the most conserved systems to maintain body homeostasis. Immune and sympathetic activities are found simultaneously increased in hypertension, suggesting a synergistic action of these two systems in the progression of this disease. Microglia, the primary immune cells in the central nervous system, have been suggested in the regulation of sympathetic outflow; depletion of microglia alters neuroinflammation and pressor responses in hypertensive models. In this review, we firstly updated the current understanding on microglial ontogeny and functions in both steady state and diseases. Then we reviewed on the interaction between autonomic nervous system and peripheral immunity in hypertension. Microglia bridge the central and peripheral inflammation through regulating the sympathetic nerve activity in hypertension. Future exploration of the molecular linkage of this pathway may provide novel therapeutics for hypertension and its related cardiovascular diseases.

19.
Ecotoxicol Environ Saf ; 185: 109705, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31561080

RESUMO

Acid rain is a global environmental problem. Acid rain can affect plants directly by damaging the leaves and indirectly by soil acidifying. Many studies have been conducted to investigate the impacts of acid rain on plant under a single soil type. However, there is little information on the effect of acid rain on plant under different soil types. Jatropha curcas L. is an energy plant widely distributed in acid rain pollution area with various soil types. In this study, we investigated the effects of acid rain (pH2.5, pH3.5, pH4.5, pH5.6) on the growth, physiology, nutrient elements and bacterial community of J. curcas seedlings under different soil types [Red soils (RS), Yellow soils (YS), Yellow-brown soils (YBS), and Purplish soils (PS)]. Acid rain and soil types significantly influence the growth of J. curcas seedlings, and there was a significant interaction between acid rain and soil types. Acid rain (pH 4.5) was beneficial to the growth of J. curcas seedlings, whereas acid rain (pH 2.5 or 3.5) inhibited growth of J. curcas seedlings. The growth of J. curcas seedlings could resist the stress of acid rain by scavenging and detoxification of active oxygen species in leaves. Combined with the increase in relative growth rate of seedlings treated with simulated acid rain at pH 4.5, we inferred that K can stimulate the growth of seedlings. The lower soil pH, cation exchange capacity and base saturation had stronger inhibitory effects on growth of J. curcas seedlings. YBS and PS were beneficial for growth of J. curcas seedlings by higher buffering capacity under acid rain treatments. The phylum Proteobacteria was found to predominate in rhizosphere soils. YBS was favorable to support Proteobacteria growth and reproduction. The redundancy analysis showed that the Cyanobacteria were favorable to growth of J. curcas seedlings.

20.
J Cell Mol Med ; 23(11): 7222-7232, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31513352

RESUMO

This study aimed to determine long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 (SNHG14) expression in pancreatic cancer and to explore the potential molecular actions of SNHG14 in mediating pancreatic cancer progression. Gene expression was detected by quantitative real-time PCR. Cell proliferation, growth and invasion were detected by respective CCK-8, colony formation, and transwell invasion assays. Protein levels were measured by Western blotting. Cell apoptosis and caspase-3 activity were detected by flow cytometry and caspase-3 activity assay. The link between miR-613 and its targets was evaluated by luciferase reporter assay. In vivo tumour growth was evaluated using a xenograft model of nude mice. SNHG14 expression was up-regulated in cancerous tissues from pancreatic cancer patients. High expression of SNHG14 was associated with poor tumour differentiation, advanced TNM stage and nodal metastasis. SNHG14 overexpression enhanced cell proliferative, growth and invasive abilities, and suppressed apoptotic rates and caspase-3 activity in pancreatic cancer cells, while SNHG14 knockdown exerted opposite effects. Mechanistic studies revealed that miR-613 was targeted by SNHG14, and Annexin A2 (ANXA2) was targeted and inversely regulated by miR-613 in pancreatic cancer cells. In vivo studies showed that SNHG14 knockdown attenuated tumour growth. MiR-613 was down-regulated and ANXA2 was up-regulated in the pancreatic cancer tissues, and SNHG14 expression levels were inversely correlated with miR-613 expression levels and positively correlated with the ANXA2 mRNA expression levels. Collectively, our results suggest that SNHG14 potentiates pancreatic cancer progression through modulation of annexin A2 expression via acting as a competing endogenous RNA for miR-613.

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