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1.
Ecotoxicol Environ Saf ; 230: 113150, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34999340

RESUMO

Contamination of microplastics (MPs) is a global environmental issue that has received much attention from the scientific and public communities due to ecological concerns in recent decades. Comparing with aquatic ecosystems, soil systems, regardless of the high importance and complexity, have been less studied under widely existing and increasing MP contamination. This review, combined with data assimilation and meta-analysis methods, has summarized current contamination conditions of soil MPs across different sites reported in earlier studies. While performing this meta-analysis, we investigated the effects of MPs on soil biota including their numbers, biomass, diversity, and physiological properties. The results showed that abundance of soil MPs ranged from 0.34 to 410958.9 items kg-1 and concentration ranged from 0.002 to 67500 mg kg-1 across sites, with agricultural soils containing significantly lower abundance and concentration of MPs than others. Presence of MPs significantly decreased the individual number of soil biota, operational taxonomic unit, diversity index (Simpson), movement index and reproduction rate, whereas the mortality rate was significantly increased by the soil MPs. Despite these significant effects, MPs did not significantly alter the biomass of soil biota, which could be due to a counteraction of their negative and positive effects on different groups of soil organisms. Moreover, we observed that soil MPs could significantly increase the Chao1 index, suggesting that MPs may act as a food resource for the soil rare biosphere. Based on the existing knowledge, we suggest that future studies should focus on research areas that include but are not limited to methodological improvements, intensive field investigations, risk assessment from the perspective of soil food web and bioaccumulation, MPs induced antibiotic resistance, and restoration strategies to reduce their concentrations in soil.

2.
Int J Mol Sci ; 23(1)2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-35008941

RESUMO

Poplar is an illustrious industrial woody plant with rapid growth, providing a range of materials, and having simple post-treatment. Various kinds of environmental stresses limit its output. Plant annexin (ANN) is a calcium-dependent phospholipid-binding protein involved in plant metabolism, growth and development, and cooperatively regulating drought resistance, salt tolerance, and various stress responses. However, the features of the PtANN gene family and different stress responses remain unknown in poplar. This study identified 12 PtANN genes in the P. trichocarpa whole-genome and PtANNs divided into three subfamilies based on the phylogenetic tree. The PtANNs clustered into the same clade shared similar gene structures and conserved motifs. The 12 PtANN genes were located in ten chromosomes, and segmental duplication events were illustrated as the main duplication method. Additionally, the PtANN4 homogenous with AtANN1 was detected localized in the cytoplasm and plasma membrane. In addition, expression levels of PtANNs were induced by multiple abiotic stresses, which indicated that PtANNs could widely participate in response to abiotic stress. These results revealed the molecular evolution of PtANNs and their profiles in response to abiotic stress.

3.
Int J Biol Macromol ; 202: 468-481, 2022 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-35063485

RESUMO

Plant non-specific lipid transfer proteins (nsLTPs) are involved in various physiological processes. However, the characteristics and function of LTPs in Populus trichocarpa are unclear. Here, we report the functional properties of type IV, V, and VI P. trichocarpa nsLTPs (PtLTPs). The IV, V, and VI PtLTPs clustered in the same clade shared similar gene structures and motif and distributions. Also, collinearity analysis revealed 2 and 7 gene pairs have tandem duplication and segmental duplication events, respectively. The expression patterns of type IV, V, and VI PtLTPs differed among poplar tissues. We investigated the effects of various stresses on the Potri.010G100600, Potri.010G196300, and Potri.016G104300 (type V LTPs) mRNA levels, and type V LTPs can respond to multiple stresses. Potri.008G061800 was localized to the cell wall, extracellular space, and plasma membrane. Glutathione-S-transferase-Potri.008G061800 obtained by prokaryotic expression had weakly inhibited the growth of Septotis populiperda in vitro. Taken together, our data show that type IV, V, and VI PtLTPs may be thought as novel regulators of plant stresses. They could be considered an effective genetic resource for molecular breeding in poplar.

4.
Gastrointest Endosc ; 2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-35016893

RESUMO

BACKGROUND AND AIMS: The current methods for treatment of giant gastric bezoars mainly include chemical dissolution, endoscopic fragmentation, and surgical removal, which often have limited curative effects or generate multiple adverse events. Therefore, there is an urgent need to find new methods to overcome such a dilemma. The aim of this study was to evaluate the safety, efficacy and feasibility of a novel guidewire-based tangential sawing fragmentation (GTSF) technique to treat giant gastric bezoars. METHODS: The retrospective single-center study was performed in the Department of Gastroenterology and Hepatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital. Ten patients with giant bezoars were consecutively enrolled from December 8, 2019 to April 8, 2021. We treated the 10 patients with the GTSF technique, recorded the GTSF procedure, and followed up the patients with gastroscopy in 2 weeks after the procedure. RESULTS: All the patients were successfully treated by the GTSF technique and the giant bezoar was broken into small pieces (< 2 cm in diameter). The average operation time was 21.73 minutes, and the average fragmentation time was 8.06 minutes. Ten patients treated with the GTSF technique obtained satisfactory treatment results, no acute adverse events occurred during the procedure, no alimentary canal injury and no bezoar residues left evidenced by gastroscopy after 2 weeks of the procedure. CONCLUSION: GTSF technique is a safe, effective, and feasible method for removing giant bezoars, and can be considered as an alternative treatment of this disease.

5.
Bioconjug Chem ; 33(1): 248-259, 2022 01 19.
Artigo em Inglês | MEDLINE | ID: mdl-34936326

RESUMO

Nanozymes are nanomaterials with enzyme-like activities, which have been developed for inflammatory disease therapy by reactive oxygen species (ROS) scavenging. The application of nanozymes in ulcerative colitis (UC) treatment not only inherits the merits of small molecular antioxidants (e.g., 5-aminosalicylic acid) to scavenge ROS but also achieves catalytic recycle instead of stoichiometric consumption. However, current therapies usually ignore the repair of mucosa, the first line of defense, whose damage increases the risk of infections. Herein, a multifunctional nanozyme hydrogel is designed and verified both as an ROS scavenger and a mucosal healing enhancer for UC therapy. The chitosan-coated CeO2 nanozyme (CCNZ) not only possesses excellent ROS-scavenging ability but also exhibits satisfactory antibacterial capacity. After gelation with alginate, the optimized CCNZ1:Alg1.5 nanozyme hydrogel exhibits multiple functions, including inflamed site targeting, supporting cell growth, ROS scavenging, and antibacterial activity, which alleviates UC better than a clinical medication 5-aminosalicylic acid by even a single-dose treatment. This study reveals that a nanozyme providing mucosal healing is promising for UC therapy with excellent potential for clinical application and enriches the nanozyme research of treatment for diseases.

6.
Bioact Mater ; 7: 242-253, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34466730

RESUMO

Bone regeneration is a crucial part in the treatment of periodontal tissue regeneration, in which new attempts come out along with the development of nanomaterials. Herein, the effect of cerium oxide nanoparticles (CeO2 NPs) on the cell behavior and function of human periodontal ligament stem cells (hPDLSCs) was investigated. Results of CCK-8 and cell cycle tests demonstrated that CeO2 NPs not only had good biocompatibility, but also promoted cell proliferation. Furthermore, the levels of alkaline phosphatase activity, mineralized nodule formation and expressions of osteogenic genes and proteins demonstrated CeO2 NPs could promote osteogenesis differentiation of hPDLSCs. Then we chose electrospinning to fabricate fibrous membranes containing CeO2 NPs. We showed that the composite membranes improved mechanical properties as well as realized release of CeO2 NPs. We then applied the composite membranes to in vivo study in rat cranial defect models. Micro-CT and histopathological evaluations revealed that nanofibrous membranes with CeO2 NPs further accelerated new bone formation. Those exciting results demonstrated that CeO2 NPs and porous membrane contributed to osteogenic ability, and CeO2 NPs contained electrospun membrane may be a promising candidate material for periodontal bone regeneration.

7.
Nano Lett ; 22(1): 508-516, 2022 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-34968071

RESUMO

Uricase-based therapies are limited for gout partially due to the accumulation of H2O2 in an arthrosis environment with slow metabolism. To tackle this limitation, previous studies adopted a cascade reaction between the degradation of uric acid (UA) and timely elimination of H2O2 using complicated composites of uricase and catalase (CAT)/CAT-like nanozyme. Herein, the self-cascade nanozyme Pt/CeO2 with high efficiency toward simultaneous UA degradation and H2O2 elimination is demonstrated on the basis of both uricase- and CAT-like activities in Pt, Ir, Rh, and Pd platinum-group metals. With an optimized molar ratio of Pt and CeO2, Pt/CeO2 (1/5) not only does better in degrading UA but also has excellent reactive oxygen species (ROS) and reactive nitrogen species (RNS) scavenging activities. In monosodium urate (MSU)-induced acute gout rats, Pt/CeO2 nanozyme markedly alleviates pain along with joint edema, thus improving gait claudication and tissue inflammation. These results provide novel insights into strategies of an efficient enzyme-mimetic treatment for gout.

8.
Ann Anat ; 239: 151820, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34411706

RESUMO

BACKGROUND: The retraction and compression of gingival tissue have a significant impact on the efficiency and stability of orthodontic treatment, but the underlying molecular mechanism has not been fully elucidated. The aim of the current study was to investigate the effects of mechanical forces on the expression level of calreticulin (CRT), the activity of the calcineurin (CaN)/nuclear factor of activated T cells (NFAT) 3 signalling pathway, and extracellular matrix (ECM) synthesis in human gingival fibroblasts (HGFs) cultured on three-dimensional (3D) poly(lactic-co-glycolic acid) (PLGA) scaffolds and to further explore the mechanical transduction pathways that may be involved. MATERIALS AND METHODS: A mechanical force of 25 g/cm2 was applied to HGFs for 0, 6, 24, 48, or 72 h. The expression of CRT, CaN, NFAT3, phosphorylated NFAT3 (p-NFAT3) and type I collagen (COL-I) were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. Subsequently, small interfering RNA (siRNA) was used to knock down the expression of CRT in HGFs, and the impacts of the applied force on the expression levels of CaN, NFAT3, p-NFAT3, and COL-I were also evaluated by RT-qPCR and western blotting. RESULTS: The application of mechanical force on HGFs cultured on 3D PLGA scaffolds led to a significant increases in CRT, CaN, and COL-I expression as well as a decrease in p-NFAT3 expression. However, the effects of mechanical force on CaN, p-NFAT3, and COL-I expression were reversed following downregulation of CRT and displayed a significant decrease in CaN/NFAT3 activity and COL-I synthesis. CONCLUSION: This study showed that the CaN/NFAT3 signalling pathway and CRT appear to be involved in the mechanotransduction of HGFs, and downregulation of CRT inhibits COL-I synthesis potentially via the CaN/NFAT3 signalling pathway. Taken together, these findings ultimately provide novel insight into the mechanisms underlying mechanical force-induced ECM synthesis, which may be conducive to the development of targeted therapeutics to treat fibrotic diseases, including gingival fibrosis caused by orthodontic treatment.


Assuntos
Calreticulina , Matriz Extracelular , Fibroblastos , Gengiva/citologia , Mecanotransdução Celular , Calcineurina , Calreticulina/genética , Células Cultivadas , Matriz Extracelular/metabolismo , Fibroblastos/metabolismo , Inativação Gênica , Humanos , Fatores de Transcrição NFATC , Copolímero de Ácido Poliláctico e Ácido Poliglicólico
9.
Anal Chem ; 2021 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-34870985
10.
Front Oncol ; 11: 703074, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34912702

RESUMO

Purpose: The aim of the study was to compare the clinical outcomes of induction chemotherapy (IC) followed by definitive concurrent chemoradiotherapy (dCCRT) versus chemoradiotherapy alone in patients with esophageal squamous cell carcinoma (ESCC) on the basis of a clinical scoring model. Methods: A retrospective review of 599 patients with ESCC treated with dCCRT at our institution from 2010 to 2019 was conducted. The patients were divided into two groups based on whether they received IC. A clinical scoring model was performed using the significant variables obtained from the multivariate analysis. The PFS and OS rates were estimated using the Kaplan-Meier method. Results: During the study period, 182 patients receiving IC followed by dCCRT and 417 dCCRT alone were identified. No significant differences in the PFS and OS rates were observed between the IC group (P=0.532) and the non-IC group (P=0.078). A clinical scoring model was constructed based on independent prognostic factors with scores ranging from 0 to 10.4. The patients were divided into high- and low-risk groups by using the median score as the cutoff value. The PFS rate of patients receiving IC was higher than that of patients treated without IC (P=0.034), while there was no improvement in the OS rate (P=0.794) in the high-risk group. No significant differences in the PFS (P=0.207) or OS (P=0.997) rate were found between the two treatment groups in the low-risk group. Conclusions: The addition of IC followed by dCCRT for patients with ESCC might be associated with better PFS rates based on a clinical scoring model but has no impact on OS rates. Further prospective studies are warranted for the validation of this model.

11.
Trials ; 22(1): 905, 2021 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-34895306

RESUMO

BACKGROUND: Chronic subdural haematoma (CSDH) is a common condition in the elderly that often requires neurosurgical management. For small CSDH, evidence has emerged that statins may reduce haematoma volume and improve outcomes, presumably by reducing local inflammation and promoting vascular repair. We wish to extend this evidence in a study that aims to determine the efficacy and safety of atorvastatin combined with low-dose dexamethasone in patients with CSDH. METHODS: The second ATorvastatin On Chronic subdural Hematoma (ATOCH-II) study is a multi-centre, randomized, placebo-controlled, double-blind trial which aims to enrol 240 adult patients with a conservative therapeutic indication for CSDH, randomly allocated to standard treatment with atorvastatin 20 mg combined with low-dose dexamethasone (or matching placebos) daily for 28 days, and with 152 days of follow-up. The primary outcome is a composite good outcome defined by any reduction from baseline in haematoma volume and survival free of surgery at 28 days. Secondary outcomes include functional outcome on the modified Rankin scale (mRS) and modified Barthel Index at 28 days, surgical transition and reduction in haematoma volumes at 14, 28 and 90 days. DISCUSSION: This multi-centre clinical trial aims to provide high-quality evidence on the efficacy and safety of the combined treatment of atorvastatin and low-dose dexamethasone to reduce inflammation and enhance angiogenesis in CSDH. TRIAL REGISTRATION: ChiCTR, ChiCTR1900021659 . Registered on 3 March 2019, http://www.chictr.org.cn/showproj.aspx?proj=36157 .


Assuntos
Hematoma Subdural Crônico , Adulto , Idoso , Atorvastatina/efeitos adversos , Dexametasona/efeitos adversos , Método Duplo-Cego , Hematoma Subdural Crônico/diagnóstico por imagem , Hematoma Subdural Crônico/tratamento farmacológico , Humanos , Estudos Multicêntricos como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
12.
Front Cardiovasc Med ; 8: 767514, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34950714

RESUMO

Background: The predictability and long-term outcome of the discrete pre-potential (DPP) of idiopathic ventricular arrhythmias (VAs) arising from the aortic sinuses of Valsalva (ASV) have not been fully identified. Methods: Of 687 consecutive patients undergoing ablation of outflow tract VAs, there were 105 (15.3%) patients with VAs originating from the ASV region who were included. Detailed mapping was performed within the ASV in all patients. Electrocardiographic, electrophysiological parameters, and long-term success rate were compared between patients with and without the DPPs. Results: A DPP was recorded in 67 of 105 (63.8%) patients, including 38 left sinus of Valsalva (LSV)-VAs (38/105, 36.2%) and 29 right sinus of Valsalva (RSV)-VAs (29/105, 27.6%). The patients with DPPs had wider QRS duration (152 ± 17 vs. 145 ± 14 ms, p < 0.001). The average of earliest activation time was significantly earlier in patients with DPPs (-38.6 ± 8.5 vs. -27.7 ± 5.7 ms, p < 0.001). Mean time from the first lesion to elimination of VAs was shorter in patients with DPPs (2.3 ± 2.1 s vs. 4.9 ± 1.0 s, p < 0.001). A stepwise logistic multivariable analysis identified only younger age as a significant predictor of DPP (age ≤ 35.5 years predicted DPP with 92.9% positive predictive value). During a follow-up duration of 42.5 ± 22.3 months, 63 (94.0%) patients with DPPs and 30 (78.9%) patients without DPPs remained free of recurrent VAs (p = 0.027). Conclusion: Discrete pre-potentials were observed in 63.8% of patients with VAs arising from the ASV. Ablation in patients with DPPs was associated with higher long-term success. DPPs were seen more commonly in younger (age ≤ 35.5 years) patients.

13.
Environ Entomol ; 50(6): 1455-1465, 2021 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-34918074

RESUMO

The Hawaiian flower thrips, Thrips hawaiiensis (Morgan), a common flower-inhabiting thrip, is now a potential pest globally. Effective control of T. hawaiiensis requires information about the effects of temperature on its ontogeny and population growth. In this study, the life history characteristics and demography of T. hawaiiensis were defined at eight temperatures (9-35°C). Additionally, the thermal constant and temperature threshold were estimated by regression analysis. The developmental duration and longevity of T. hawaiiensis decreased with an increase in temperature between 16°C and 32°C; females survived for longer than males at all temperatures. The lower temperature threshold and thermal constant of preadult T. hawaiiensis were 10.5°C and 132.5 degree-days, respectively. The oviposition days of the females gradually decreased from 16°C to 32°C, and net maternity was higher at 20°C than at 16°C, even though the same number of eggs were laid at both temperatures. The mean longevities of the populations were greatest at 20°C; the life expectancy and reproductive value decreased with temperature. The intrinsic rate of increase and finite rate of increase were significantly highest at 20°C, 25°C, and 30°C. Population growth was triggered at 12.3°C, and reached a peak at approximately 27°C when it proliferated to the largest population size. Therefore, the results suggest that although the population of T. hawaiiensis starts to grow at lower temperatures, it adapts to a wide range of temperatures, and these findings facilitate prediction of different stages of damage, population size, and seasonal occurrence of T. hawaiiensis.

14.
Cell Mol Biol Lett ; 26(1): 52, 2021 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-34895159

RESUMO

BACKGROUND: The study was designed to elucidate the association and functional roles of miR-26b-5p and c-MYC binding protein (MYCBP) in triple-negative breast cancer (TNBC). METHOD: Luciferase reporter assay was used to confirm the relationship between miR-26b-5p and MYCBP in TNBC cells. The expression levels of miR-26b-5p and MYCBP in tissue specimens and cell lines were determined using reverse transcription-quantitative PCR. Cell proliferation, migration and invasion were assessed using CCK-8 assay, colony formation and transwell assay. RESULTS: We first observed that miR-26b-5p directly targets the 3'-UTR of MYCBP to inhibit MYCBP expression in MDA-MB-468 and BT-549 cells. The expression of miR-26b-5p was inversely correlated with MYCBP expression in TNBC tissues. We further demonstrated that MYCBP knockdown suppressed the proliferation, migration and invasion of TNBC cells. Furthermore, MYCBP overexpression counteracted the suppressive effect of miR-26b-5p on TNBC cell behaviors. Western blot analysis demonstrated that the E-cadherin protein level was increased, while protein levels of N-cadherin and vimentin were decreased in cells transfected with miR-26b-5p, which were all reversed by ectopic expression of MYCBP. CONCLUSIONS: In summary, our findings revealed the tumor suppressive role of miR-26b-5p in regulating TNBC cell proliferation and mobility, possibly by targeting MYCBP.

15.
Anal Chem ; 93(45): 15150-15158, 2021 11 16.
Artigo em Inglês | MEDLINE | ID: mdl-34738799

RESUMO

Mimicking enzyme specificity via construction of on-demand geometric structures on nanozymes is of great interest in recent years. Although building substrate-specific polymers on nanozymes has achieved great success, polymer-blocked active sites would inevitably lead to decreased activity of nanozymes. Here, we have developed three photoactive metal-organic framework (MOF)-based nanozymes (called 2D-TCPP, 3D-TCPP, and AD-TCPP), which have different geometric structures as well as unshielded active sites. Together with their structural variations and excellent photoresponsive oxidase-like activities, these photoactive nanozymes exhibit structure-dependent specificity for three kinds of substrates (typical oxidase substrates, organic pollutants, and antioxidants). Moreover, AD-TCPP and 3D-TCPP show potential applications for environmental protection and bioanalysis, respectively. This work offers a promising approach to the development of nanozymes with enzyme-like specificity.


Assuntos
Estruturas Metalorgânicas , Nanoestruturas , Catálise , Oxirredutases , Especificidade por Substrato
16.
Nat Commun ; 12(1): 6866, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34824234

RESUMO

The activity of nanomaterials (NMs) in catalytically scavenging superoxide anions mimics that of superoxide dismutase (SOD). Although dozens of NMs have been demonstrated to possess such activity, the underlying principles are unclear, hindering the discovery of NMs as the novel SOD mimics. In this work, we use density functional theory calculations to study the thermodynamics and kinetics of the catalytic processes, and we develop two principles, namely, an energy level principle and an adsorption energy principle, for the activity. The first principle quantitatively describes the role of the intermediate frontier molecular orbital in transferring electrons for catalysis. The second one quantitatively describes the competition between the desired catalytic reaction and undesired side reactions. The ability of the principles to predict the SOD-like activities of metal-organic frameworks were verified by experiments. Both principles can be easily implemented in computer programs to computationally screen NMs with the intrinsic SOD-like activity.

17.
Biology (Basel) ; 10(11)2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34827184

RESUMO

Anthropogenic nitrogen (N) and sulfur (S) deposition can change above- and belowground biodiversity, including soil microbial diversity. The diversity of denitrifying microorganisms is of great significance to the calculation of the global nitrogen cycle and nitrogen flux. For a long time, nirK and nirS have been used as the functional genes to study denitrifying microorganisms, and have gradually become molecular markers for studying the composition and diversity of denitrifying bacteria. Here, three-time exposures to N and S applications (7, 30, and 60 days), were independently established. Additionally, the abundance, diversity, and structure of nirK- and nirS-type denitrifying communities were examined by sequencing analyses in response to three treatments, namely, N and S (TN/S), sodium chloride (TNaCl) and deionized water (pH = 7.0) (CK). Our results suggest that TN/S led to higher electrical conductivity (EC), total nitrogen (TN), total organic carbon (TOC), nitrate nitrogen (NO3--N), ammonium nitrogen (NH4+-N), and lower pH compared with TNaCl and CK, which affected the diversity of nirK- and nirS-type denitrifying bacterial communities. We also observed that the nirK-type denitrifying community demonstrated a higher sensitivity to N and S additions. Overall, our results are important for the understanding of nitrogen in soil and N2O emissions.

18.
Genes (Basel) ; 12(11)2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34828321

RESUMO

Tumor mutational burden (TMB) is considered a potential biomarker for predicting the response and effect of immune checkpoint inhibitors (ICIs). To find specific gene mutations related to TMB and the prognosis of patients, the frequently mutated genes in gastric cancer patients from TCGA and ICGC were obtained and the correlation between gene mutation, TMB, and prognosis was analyzed. Furthermore, to clarify whether specific gene mutations can be used as predictive biomarkers of ICIs, a gene set enrichment analysis (GSEA) for immune pathways and an immune infiltration analysis were conducted. The results showed that CUB and Sushi multiple domains 1 (CSMD1) mutation (CSMD1-mut) were associated with higher TMB and better prognosis in patients. The genetic map showed that, compared with wild-type samples, the loss of chromosomes 4q, 5q, 8p, and 9p decreased and the status of microsatellite instability increased in the CSMD1-mut samples. The GSEA analysis showed that immune-related pathways were enriched in the CSMD1-mut samples. The immune infiltration analysis showed that the anti-tumor immune cells were upregulated and that the tumor-promoting immune cells were downregulated in the CSMD1-mut samples. The gene co-expression analysis showed that PD-L1 expression was higher in the CSMD1-mut samples. In summary, CSMD1-mut in gastric cancer was associated with increased TMB and favorable survival and may have potential significance in predicting the efficacy of anti-PD-L1.

19.
Mar Pollut Bull ; 173(Pt B): 113145, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34800761

RESUMO

Here, we examined the 48-h acute toxicity of cadmium (Cd) in the marine copepod Tigriopus japonicus under two pCO2 concentrations (400 and 1000 µatm). Subsequently, T. japonicus was interactively exposed to different pCO2 (400, 1000 µatm) and Cd (control, 500 µg/L) treatments for 48 h. After exposure, biochemical and physiological responses were analyzed for the copepods. The results showed that the 48-h LC50 values of Cd were calculated as 12.03 mg/L and 9.08 mg/L in T. japonicus, respectively, under 400 and 1000 µatm pCO2 conditions. Cd exposure significantly promoted Cd exclusion/glycolysis, detoxification/stress response, and oxidative stress/apoptosis while it depressed that of antioxidant capacity. Intriguingly, CO2-driven acidification enhanced Cd bioaccumulation and its toxicity in T. japonicus. Overall, our study provides a mechanistic understanding about the interaction between seawater acidification and Cd pollution in marine copepods.

20.
Dalton Trans ; 50(46): 17202-17207, 2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34783818

RESUMO

A range of titanium compounds containing the naturally occurring dyes quinizarin (QH2) and alizarin (AH2) was synthesized and structurally characterized in the solid state. Among these is the first examples of a discrete metallocyclic arrangement formed exclusively using quinizarin ligands and the first examples of lanthanide containing titanium compounds of the alizarin family of ligands.

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