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1.
Brain Behav ; 9(6): e01290, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31007001

RESUMO

OBJECTIVES: Proinflammatory cytokines triggered by surgery and postoperative pain are major causes of postoperative delirium (POD). This study investigated the effects of flurbiprofen axetil on POD when used for postoperative analgesia after major noncardiac surgery in elderly patients. METHODS: Patients over 65 years old were randomly divided into two groups: the sufentanil group (S group), in which 150 µg of sufentanil was used in the patient-controlled analgesia (PCA) pump for 3 days; the sufentanil combined with flurbiprofen axetil group (SF group), in which 150 µg of sufentanil was combined with 300 mg of flurbiprofen axetil in the PCA pump for 3 days. The Confusion Assessment Method scale was used for POD evaluation. The pain intensity, side effects, and risk factors (age, gender, surgical position, and category of surgery) for POD were evaluated. RESULTS: Ultimately, 140 patients were included. The overall incidence of POD was not significantly different between the S and SF groups. The incidence of POD was significantly lower in the SF group than in the S group among patients over 70 years (5.1% vs. 20.7%, p = 0.045, odds ratio = 0.146, 95% confidence interval = 0.020-1.041). The incidence of POD was no difference in patients classified by the category of surgery, surgical position, or gender between groups. Sufentanil and flurbiprofen axetil in the PCA pump was completely used within 72 hr. The pain intensity, consumed sufentanil dosage of the PCA, and the side effects was not different between groups. CONCLUSIONS: Flurbiprofen axetil might reduce POD in patients over 70 years undergoing major noncardiac surgery.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Delírio/prevenção & controle , Flurbiprofeno/análogos & derivados , Complicações Pós-Operatórias/prevenção & controle , Sufentanil/administração & dosagem , Idoso , Analgésicos Opioides/administração & dosagem , Delírio/induzido quimicamente , Método Duplo-Cego , Quimioterapia Combinada , Feminino , Flurbiprofeno/farmacologia , Humanos , Masculino , Dor Pós-Operatória/tratamento farmacológico , Estudos Prospectivos
2.
Biomed Pharmacother ; 110: 818-824, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30554120

RESUMO

INTRODUCTION: It has been demonstrated HOE-642 ameliorates ischemic contracture, prevents post-resuscitation diastolic dysfunction, and favors the earlier return of contractile function. This study is the first report to explore the optimal dose of HOE-642 in protecting the neuronal mitochondrial function after cardiac arrest. METHODS: Cardiac arrest was induced by 8 min asphyxia in rats. There were Sham (S), Normothermic (NORM), and Hypothermic (HYPO) groups. The NORM or HYPO groups consist of four subgroups: NORM/HYPO + HOE-642 0, 1, 3, and 5 mg/kg. Survival and NDS were evaluated after 24 h of resuscitation. ΔΨm, mitochondrial swelling, ROS production, and mitochondrial complex IIV activity of the hippocampus were detected. RESULTS: Survival in the HYPO + 1 mg group was the best and significantly higher than in the NORM + 0 mg and NORM + 1 mg groups. NDS in the HYPO + 0 mg, HYPO + 1 mg, and HYPO + 3 mg groups was significantly lower than in the NORM + 0 mg group. ΔΨm in the NORM + 1 mg (n = 5) group was significantly higher than in the NORM + 0 mg (n = 8), NORM + 3 mg (n = 5), and NORM + 5 mg (n = 5) groups. The ROS production in the NORM + 1 mg and NORM + 3 mg groups were significantly lower than in the NORM + 0 mg and NORM + 5 mg groups. Complex I and III activities in the HYPO + 1 mg (n = 5) group were significantly higher than in the HYPO + 3 mg (n = 5), and HYPO + 5 mg (n = 5) groups. Complex II and IV activities in the NORM + 3 mg and HYPO + 3 mg groups were significantly higher than in the NORM + 0 mg, NORM + 1 mg, and HYPO + 0 mg (n = 4)groups. CONCLUSIONS: HOE-642 1 or 3 mg/kg showed benefits compared to HOE-642 5 mg/kg used when initiating resuscitation. When combined with hypothermia after cardiac arrest, HOE-642 1 or 3 mg/kg improved survival and neurological function compared with hypothermia or HOE-642 alone, however, HOE-642 5 mg/kg plus hypothermia did not.


Assuntos
Antiarrítmicos/uso terapêutico , Cardiotônicos/uso terapêutico , Guanidinas/uso terapêutico , Parada Cardíaca/prevenção & controle , Mitocôndrias/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Sulfonas/uso terapêutico , Animais , Antiarrítmicos/farmacologia , Cardiotônicos/farmacologia , Relação Dose-Resposta a Droga , Guanidinas/farmacologia , Parada Cardíaca/metabolismo , Parada Cardíaca/patologia , Masculino , Mitocôndrias/metabolismo , Mitocôndrias/patologia , Neurônios/metabolismo , Neurônios/patologia , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/antagonistas & inibidores , Espécies Reativas de Oxigênio/metabolismo , Sulfonas/farmacologia
3.
Nat Commun ; 9(1): 3809, 2018 09 18.
Artigo em Inglês | MEDLINE | ID: mdl-30228308

RESUMO

Ferroelectrics, which generate a switchable electric field across the solid-liquid interface, may provide a platform to control chemical reactions (physical properties) using physical fields (chemical stimuli). However, it is challenging to in-situ control such polarization-induced interfacial chemical structure and electric field. Here, we report that construction of chemical bonds at the surface of ferroelectric BiFeO3 in aqueous solution leads to a reversible bulk polarization switching. Combining piezoresponse (electrostatic) force microscopy, X-ray photoelectron spectroscopy, scanning transmission electron microscopy, first-principles calculations and phase-field simulations, we discover that the reversible polarization switching is ascribed to the sufficient formation of polarization-selective chemical bonds at its surface, which decreases the interfacial chemical energy. Therefore, the bulk electrostatic energy can be effectively tuned by H+/OH- concentration. This water-induced ferroelectric switching allows us to construct large-scale type-printing of polarization using green energy and opens up new opportunities for sensing, high-efficient catalysis, and data storage.

4.
Sci Rep ; 8(1): 10161, 2018 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-29977080

RESUMO

This study assessed the efficacy and tolerability of intravenous ibuprofen in the improvement of post-operative pain control and the reduction of opioid usage. Patients were randomly divided into placebo, ibuprofen 400 mg and ibuprofen 800 mg groups. All patients received patient-controlled intravenous morphine analgesia after surgery. The first dose of study drugs was administered intravenously 30 min before the end of surgery and then every 6 hours, for a total of 8 doses after surgery. The primary endpoint of this study was the mean amount of morphine used during the first 24 hours after surgery. Morphine use was reduced significantly in the ibuprofen 800 mg group compared with the placebo group (P = 0.04). Tramadol use was reduced significantly in the ibuprofen 400 mg and ibuprofen 800 mg groups compared with the placebo group (P < 0.01). The area under the curve of visual analog scale pain ratings was not different between groups. Safety assessments and side effects were not different between the three groups. Intravenous ibuprofen 800 mg was associated with a significant reduction in morphine requirements, and it was generally well tolerated for postoperative pain management in patients undergoing radical cervical cancer surgery.


Assuntos
Dor Aguda/tratamento farmacológico , Analgésicos Opioides/uso terapêutico , Ibuprofeno/administração & dosagem , Dor Pós-Operatória/tratamento farmacológico , Dor Pós-Operatória/etiologia , Neoplasias do Colo do Útero/cirurgia , Administração Intravenosa , Método Duplo-Cego , Feminino , Humanos , Ibuprofeno/efeitos adversos , Ibuprofeno/uso terapêutico , Pessoa de Meia-Idade , Medição da Dor , Placebos , Estudos Prospectivos , Tramadol/uso terapêutico , Resultado do Tratamento
5.
Sci Rep ; 7: 46089, 2017 04 06.
Artigo em Inglês | MEDLINE | ID: mdl-28383021

RESUMO

We examine the doping effects in the two-dimensional periodic Anderson model using the determinant Quantum Monte Carlo (DQMC) method. We observe bound states around the Kondo hole site and find that the heavy electron states are destroyed at the nearest-neighbor sites. Our results show no clear sign of hybridization oscillation predicted in previous mean-field calculations. We further study the electron transport with increasing doping and as a function of temperature and obtain a critical doping xc ≈ 0.6 that marks a transition from the Kondo insulator regime to the single-ion Kondo regime. The value of xc is in good agreement with the predicted threshold for the site percolation. Our results confirm the percolative nature of the insulator-metal transition widely observed in doped Kondo insulators.

6.
Artigo em Chinês | MEDLINE | ID: mdl-23593859

RESUMO

OBJECTIVE: To evaluate the effects of the magnetic particle antibody immunoassay (MPAIA), dipstick dye immunoassay (DDIA) and indirect hemagglutination assay (IHA), on detecting advanced schistosomiasis. METHODS: The sera of 224 cases of advanced schistosomiasis were detected by MPAIA, DDIA, and IHA, and the positive rates were compared. RESULTS: The positive rates of MPAIA, DDIA and IHA, were 67.14%, 14.29% and 16.52%, respectively,the positive coincidence rate of MPAIA is higher than the one of IHA and DDIA. CONCLUSION: The value of MPAIA is higher than that of DDIA or IHA in screening advanced schistosomiasis.


Assuntos
Testes de Hemaglutinação , Imunoensaio , Esquistossomose/diagnóstico , Humanos , Sensibilidade e Especificidade
7.
Artigo em Chinês | MEDLINE | ID: mdl-22164507

RESUMO

OBJECTIVE: To explore a non-invasive method for detection of urine antibodies to Schistosoma japonicum. METHODS: The urine antibodies to S. japonicum were detected by magnetic particle affinity immunoassay (MPAIA) in 158 cases of schistosomiasis japonica and 100 health persons, and their serum antibodies to S. japonicum were also detected at the same time. RESULTS: The sample of urine by MPAIA was 10 microl original urine without any special treatment. The positive rate of urine and serum were 48.10% (76/158)and 88.61% (140/158), respectively. There was difference between the performance of two methods (chi2 = 60.24, P < 0.05). However, both of their specificity were 100% (100/100). CONCLUSION: MPAIA is viable for detection of urine antibodies to S. japonicum, but its sensitivity should be improved.


Assuntos
Anticorpos Anti-Helmínticos , Imunoensaio/métodos , Magnetismo/métodos , Schistosoma japonicum/imunologia , Esquistossomose Japônica/diagnóstico , Animais , Anticorpos Anti-Helmínticos/sangue , Anticorpos Anti-Helmínticos/urina , Humanos , Imunoensaio/instrumentação , Schistosoma japonicum/isolamento & purificação , Esquistossomose Japônica/sangue , Esquistossomose Japônica/urina , Sensibilidade e Especificidade
8.
Artigo em Chinês | MEDLINE | ID: mdl-19852363

RESUMO

OBJECTIVE: To establish a magnetic particle antibody immunoassay (MPAIA) for the detection of specific antibody in sera of schistosomiasis patients. METHODS: Fluorescein isothiocyanate (FITC) was used to label Schistosoma japonicum soluble egg antigen (Sj-SEA). Anti-human IgG coated with alkaline phosphatase (ALP) as enzyme-labeled second antibody, and magnetic beads were coupled with sheep anti-FITC antibody as solid phase. Phenolphthale in monophosphate was used as substrate to set up MPAIA for the detection. Serum samples from cases with schistosomiasis or other helminth infections were tested. RESULTS: The positive rate of MPAIA was 96.7% (116/120) with the sera of S. japonicum-infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases. The positive titer with reference sample was 1: 1,600. The precision was lower than 10%. The MPAIA tips can be stored at 4 degrees C for 12 months. CONCLUSION: MPAIA shows a high sensitivity, proper specificity and long-term validity for schistosomiasis detection.


Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Contagem de Ovos de Parasitas/métodos , Schistosoma japonicum/imunologia , Animais , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/isolamento & purificação , Humanos , Magnetismo , Sensibilidade e Especificidade
9.
Artigo em Chinês | MEDLINE | ID: mdl-15281456

RESUMO

OBJECTIVE: To screen special mimic epitopes of Trichinella spiralis antigen from peptide library for exploring new diagnostic antigens. METHODS: Ts-IgG purified from serum of trichinosis patients was used to screen the phage 12-mer peptide library for 5 rounds. 24 clones were picked out randomly to detect the immunoactivity. The sensitivity and specificity of the 6 clones (T1 - T6) whose A values were higher than others were tested by ELISA. RESULTS: The sensitivity of the clones T1 - T6 was the same with larval antigen of Trichinella spiralis (TsA) (positive rate: 100%, P > 0.05), and there was no difference in specificity between T1 - T6 and TsA (negative rate: 0 - 40%, P > 0.05); T3 and T6 did not react with sera from patients of paragonimiasis, showing higher specificity than TsA (P < 0.05); T6 did not react with sera from patients of schistosomiasis, also showing higher specificity than TsA (P < 0.05). CONCLUSION: The mimic antigenic epitopes of Trichinella spiralis have been successfully obtained by screening phage 12-mer peptide library.


Assuntos
Antígenos de Helmintos/imunologia , Biblioteca de Peptídeos , Trichinella spiralis/imunologia , Triquinelose/diagnóstico , Animais , Epitopos/imunologia , Humanos , Sensibilidade e Especificidade , Triquinelose/imunologia
10.
Artigo em Chinês | MEDLINE | ID: mdl-15108515

RESUMO

OBJECTIVE: To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library. METHODS: Specific Ig was purified from sera of patients with acute schistosomiasis and used to immunoscreen the phage peptide library (PH. D.-12). After 3 rounds of panning, 10 positive plaques were selected and amplified. The immunoactivity of each clone was examined by ELISA. The sensitivity and specificity of immunoactive clones were confirmed by detecting the sera of patients with different parasitosis. RESULTS: Six clones could bind to the specific Ig purified from sera of patients with acute schistosomiasis. One clone with the highest A492 value showed a high sensitivity and specificity. CONCLUSION: The clone (SjA1) identified by the specific Ig from the library played a better part in the immunodiagnosis of schistosomiasis.


Assuntos
Antígenos de Helmintos/imunologia , Biblioteca de Peptídeos , Schistosoma japonicum/imunologia , Esquistossomose Japônica/imunologia , Animais , Epitopos/imunologia , Humanos , Imunoglobulinas/imunologia , Esquistossomose Japônica/diagnóstico , Sensibilidade e Especificidade
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