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Zhongguo Zhong Yao Za Zhi ; 44(2): 265-269, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30989943


This study is based on the data analysis of medicinal plant resources and diversity collected from the fourth Chinese traditional medicine resource survey( pilot). Through the analysis of relevant data from 33 census pioneer plots in Guizhou province( area),a total of 265 families,1 432 genera and 5 296 species of medicinal resources were reported,including algae,fungi,lichens,mosses,a total of 43 genera and 35 families,57,48 families,120 genera and 453 species of ferns,gymnosperms 11 families,22 genera and 61 species,167 families,1 243 genera and 4 721 species of angiosperms,4 genera and 4 families four medicinal animals.Compared with the data related to the third survey of traditional Chinese medicine resources,the number of ferns,gymnosperms and angiosperms in the fourth survey has increased far more than that of the third survey. From the regional distribution of medicinal resources,the composition of the genus,the type of life,and the location of the medicine,the richness of the medicinal plant resources in Guizhou province is not only reflected in many types,but also in the variety of medicinal resources. These studies provide a scientific basis for vigorously developing the Chinese herbal medicine industry and the sustainably using medicinal plant resources in Guizhou province.

Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Plantas Medicinais/classificação , China , Cycadopsida , Gleiquênias , Magnoliopsida
Zhong Yao Cai ; 37(9): 1570-3, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25857155


OBJECTIVE: To establish the HPLC fingerprint of Ardisia japonica. METHODS: HPLC analysis was performed on a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with the eluting system of gradient consisted of methanol-0.1% H3PO4. The flow rate was 1.0 mL/min. The column temperature was maintained at 35 degrees C and the detection wavelength was 250 nm. RESULTS: The HPLC fingerprint was established and 10 batches of samples with 32 common peaks were compared. Four peaks were identified as gallic acid, bergenin, chlorogenic acid and quercitrin. CONCLUSION: The method with good reproducibility is simple and accurate, which can be used for determination of HPLC fingerprint and quality control of Ardisia japonica. It provides scientific basis of future study of Ardisia japonica.

Ardisia , Cromatografia Líquida de Alta Pressão , Ácido Clorogênico , Controle de Qualidade , Quercetina/análogos & derivados , Reprodutibilidade dos Testes
Zhongguo Zhong Yao Za Zhi ; 38(16): 2577-80, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24228565


OBJECTIVE: To establish sequence characterized amplified region markers of Polygonum capitatum. METHOD: The random primer was screened through RAPD to obtain the specific RAPD marker band, and the band was separated, extracted, cloned and sequenced. The specific primers were designed for conventional PCR reaction on the basis of the specific band, and the SCAR marker was acquired. RESULT: Screening from 50 RAPD primer, only C29 primer had 2 specific bands could distinguish P. capitatum from P. nepalense, then 4 pairs of specific primers were designed based on the 2 sequences of RAPD marker bands, and only 1 pair primer (Z1-2) was successfully converted into SCAR marker after repeated tests. CONCLUSION: The Z1-2 primer, could be used as an effective SCAR mark to identify Z300 DNA for P. capitatum. The SCAR mark was established and can be used as a molecular marker to distinguish P. capitatum from P. nepalense

Polygonum/classificação , Polygonum/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Análise de Sequência de DNA , Código de Barras de DNA Taxonômico , DNA de Plantas/genética , Marcadores Genéticos/genética
J Plant Physiol ; 169(8): 770-4, 2012 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-22437146


An efficient micropropagation protocol has been developed for Dendrobium officinale, through protocorm-like bodies (PLBs). A correlation between enhanced differentiation of PLBs of D. officinale by ultrasound and changes in the levels of endogenous hormones and the antioxidant enzyme activities was described. Ultrasound treatments improved the conversion of PLBs of D. officinale to shoots. The highest conversion frequency of PLBs to shoots was obtained following the ultrasound treatment at 300 W for 5 min. Compared to the control, the enhanced conversion of PLBs to shoots following the ultrasound treatment was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA), which was due to a decrease in the endogenous level of IAA and an increase in the endogenous level of total CTKs. Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by ultrasound was associated with the inhibition of CTK decomposition by CTK oxidase (EC, while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase (EC In addition, ultrasound treatment increased the activities of superoxide dismutase (EC, catalase (EC and peroxidase (EC in the conversion process of PLBs to shoots.

Diferenciação Celular/efeitos da radiação , Dendrobium/citologia , Dendrobium/efeitos da radiação , Ondas de Choque de Alta Energia , Brotos de Planta/citologia , Brotos de Planta/efeitos da radiação , Catalase/metabolismo , Células Cultivadas , Citocininas/metabolismo , Dendrobium/crescimento & desenvolvimento , Dendrobium/metabolismo , Ácidos Indolacéticos/metabolismo , Oxirredutases/metabolismo , Peroxidase/metabolismo , Reguladores de Crescimento de Planta/metabolismo , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Superóxido Dismutase/metabolismo