Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 27
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
2.
Clin Exp Allergy ; 48(4): 424-432, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29369458

RESUMO

BACKGROUND: Celery (Apium graveolens L.) is a vegetable consumed world-wide. Celery stalks and celeriac roots are often ingredients in convenient food products like spice blends and soups. OBJECTIVE: In this study, we examined the allergenicity of distinct celeriac cultivars. METHODS: Sixteen celery-allergic patients were identified using a double-blind, placebo-controlled food challenge. Ten different celeriac cultivars were used for skin prick testing in the patients. Two cultivars were further applied for oral food challenges; their protein composition was analysed by immunoblotting, and contents of major allergen Api g 1 were quantified. RESULTS: From the 10 investigated celeriac cultivars, two cultivars elicited significantly different skin reactivity ("Anita": 5.0 [2.0-12.0] mm vs "Prinz": 7.0 [3.0-9.5] mm; P = .047). Moreover, "Anita" induced fewer symptoms after a controlled oral-celeriac challenge in 14 patient (P < .001). The protein profiles on 2DE protein gels showed distinct protein patterns and higher protein amounts of Api g 1 in "Prinz" than in "Anita." CONCLUSIONS AND CLINICAL RELEVANCE: Taken together, the data from this study suggest that cultivar Anita is better tolerated in celery-allergic patients than "Prinz." Differences in the protein expression profile between the cultivars, particularly the different content of Api g 1, could cause the different allergenicity.


Assuntos
Antígenos de Plantas/imunologia , Apium/imunologia , Hipersensibilidade Alimentar/imunologia , Proteínas de Plantas/imunologia , Adulto , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
3.
Chirurg ; 86(5): 410-8, 2015 May.
Artigo em Alemão | MEDLINE | ID: mdl-25794450

RESUMO

BACKGROUND: Complications cannot always be avoided and their treatment is an integral component of a high quality medical treatment. Complications of the central airways are rare but necessitate supportive treatment by an experienced thoracic surgeon. OBJECTIVE: The reader should become acquainted with measures to prevent complications, to recognize and treat complications early and should understand the necessity for an interdisciplinary approach. MATERIAL AND METHODS: A selective literature research was supplemented by personal experiences and complemented with prospectively collected photographs. RESULTS: There are risk constellations for the appearance of all the mentioned complications which the surgeon needs to know in order to be able to take measures for early detection of complications. Iatrogenic tracheal injuries and bronchial stump fistulae are rare (< 5 %) whereas recurrent laryngeal nerve palsy after left-sided pneumonectomy occurs in up to 30 % of cases. DISCUSSION: After the occurrence of complications at the latest, it is very important to include experienced thoracic surgeons and other specialists when necessary to protect the patient from further damage.


Assuntos
Anastomose Cirúrgica , Brônquios/lesões , Brônquios/cirurgia , Complicações Pós-Operatórias/cirurgia , Deiscência da Ferida Operatória/cirurgia , Procedimentos Cirúrgicos Torácicos/efeitos adversos , Traqueia/lesões , Paralisia das Pregas Vocais/cirurgia , Fístula Brônquica/prevenção & controle , Fístula Brônquica/cirurgia , Intervenção Médica Precoce , Humanos , Intubação Intratraqueal/efeitos adversos , Pneumonectomia/efeitos adversos , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/prevenção & controle , Estudos Prospectivos , Reoperação , Fatores de Risco , Deiscência da Ferida Operatória/prevenção & controle , Traqueia/cirurgia , Paralisia das Pregas Vocais/prevenção & controle
4.
Lung Cancer ; 88(1): 34-41, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25708529

RESUMO

OBJECTIVES: Pulmonary adenocarcinomas (ADC) can be sub-grouped based on dominant oncogenic drivers. EGFR mutations define an entity of metastatic ADC with favorable prognosis and high susceptibility to EGFR tyrosine kinase inhibition. In contrast, the clinical impact of additional ERBB family members in ADC is less defined. To this end we prospectively studied HER2 expression, gene amplification, and mutation in relation to outcome of patients with advanced or metastatic ADC. MATERIALS AND METHODS: Diagnostic tumor biopsies from 193 sequential patients with stage III/IV ADC were prospectively studied for HER2 expression by immunohistochemistry (IHC). Cases with IHC scores 2+ or 3+ were analyzed by HER2 chromogenic in situ hybridization (CISH), and sequencing of HER2 exons 20 and 23. Additional prospectively determined biomarkers included PTEN, cMET, pAKT, and pERK expression, KRAS, EGFR, BRAF and PIK3CA mutations, and ALK fluorescence ISH (FISH). RESULTS AND CONCLUSION: HER2-IHC was feasible in 176 (91.2%) cases. Of 53 (30%) cases with IHC scores 2+/3+, 45 (85%) could be studied by CISH and 34 (64%) by sequencing. The lower number of HER2-mutational analyses resulted from exhaustion of tumor tissue and DNA following mutational analysis of KRAS, EGFR, BRAF and PIK3CA. HER2 amplification was detected in 4 cases (2.3%), while no mutation was found. HER2 expression correlated with expression of pAKT and cMET. Expression of HER2 and pAKT was associated with favorable overall survival in stage IV disease. HER2-expressing ADC more frequently harbored KRAS mutations, while HER2 expression was absent in all 4 cases with BRAF mutation. HER2-IHC was not predictive of HER2 gene amplification or mutation, which both were rare events in prospectively studied patients with advanced or metastatic ADC. Expression of HER2 and pAKT define a population of patients with stage IV ADC with a distinct disease course, who could benefit from specifically tailored pharmacotherapies.


Assuntos
Adenocarcinoma/metabolismo , Biomarcadores Tumorais/metabolismo , Neoplasias Pulmonares/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Receptor ErbB-2/metabolismo , Adenocarcinoma/mortalidade , Adenocarcinoma/secundário , Idoso , Análise Mutacional de DNA , Feminino , Amplificação de Genes , Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Estudos Prospectivos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Receptor ErbB-2/genética , Transdução de Sinais
5.
J Evol Biol ; 28(2): 415-27, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25535672

RESUMO

Changes in sexual signals have the potential to promote rapid divergence and reproductive isolation among populations of animals. Thus, identifying processes contributing to variation in signals is key to understanding the drivers of speciation. However, it is difficult to identify the processes initiating changes in signals in empirical systems because (1) the demographic history of populations under study is usually unclear, and (2) there is no unified hypothesis-testing framework for evaluating the simultaneous contribution of multiple processes. A unique system for study in the Hawaiian Islands, the planthopper species Nesosydne chambersi, offers a clear demographic context to disentangle these factors. By measuring variation in male vibratory sexual signals across different genetic populations on the island of Hawaii, we found that that multiple signal traits varied significantly between populations. We developed a mixed modelling framework to simultaneously test competing hypotheses about which processes contribute to changes in signal traits: genetic drift, sensory drive or reproductive character displacement. Our findings suggest that signal divergence proceeds along different axes for different signal traits under the influence of both neutral and selective processes. They are the first, to our knowledge, to document the relative importance of multiple processes on divergence in sexual signals.


Assuntos
Hemípteros/fisiologia , Comportamento Sexual Animal/fisiologia , Comunicação Animal , Animais , Feminino , Hemípteros/genética , Masculino , Seleção Genética
6.
Clin Exp Allergy ; 43(12): 1419-27, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24118131

RESUMO

BACKGROUND: Tomato became one of the world-wide most consumed vegetables, unfortunately accompanied by an increasing risk of tomato allergy affecting certain people. As tomato allergic subjects show highly variable reactions in clinical allergy tests, it is difficult to identify cultivars or differentially treated tomato plants where a significant reduction in the allergenic potential over all subjects of a cohort can be detected. OBJECTIVE: This study was carried out to test the hypothesis that individual variability is based on differential reactions of single subjects to particular allergens in tomato fruits of plants with certain genetic background or cultivated under distinct conditions. METHODS: Proteins were extracted from tomato fruits of the previously investigated genotypes 76R, its mycorrhizal mutant RMC, and the cultivar Counter, fertilized with different forms of nitrogen in deficit or excess. 2-D immunoblots were carried out with sera of nine tomato allergic subjects, beforehand analysed in skin prick tests. RESULTS: In total, ten putative tomato allergens were identified in these immunoblots. No correlation was detected between individual skin prick test results and the quantity of positive reactions to putative allergens. IgEs of each subject showed reactions to nearly every identified putative allergen, but reactions were dependent on genotype and growth conditions. Among the ten putative tomato allergens, five new candidates were identified as follows: an endo-ß-mannanase, a pectinacetylesterase, a pectinesterase inhibitor, an aspartyl protease family protein and a protein of unknown function. CONCLUSION AND CLINICAL RELEVANCE: The hypothesis that high interindividual differences in allergic reactions are based on the interactions between the IgEs of allergic subjects with particular allergens has to be rejected. However, five proteins with putative clinical relevance as tomato allergens could be newly identified.


Assuntos
Alérgenos/imunologia , Antígenos de Plantas/imunologia , Hipersensibilidade Alimentar/imunologia , Imunoglobulina E/imunologia , Lycopersicon esculentum/efeitos adversos , Adolescente , Adulto , Alérgenos/metabolismo , Antígenos de Plantas/metabolismo , Feminino , Hipersensibilidade Alimentar/diagnóstico , Hipersensibilidade Alimentar/metabolismo , Humanos , Imunoglobulina E/metabolismo , Masculino , Proteínas de Plantas/imunologia , Proteínas de Plantas/metabolismo , Proteômica/métodos , Testes Cutâneos , Adulto Jovem
7.
Ann Oncol ; 24(11): 2866-70, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24026539

RESUMO

BACKGROUND: Endobronchial ultrasound with transbronchial needle aspiration (EBUS-TBNA) is a well-established method to assess mediastinal lymph nodes for lung cancer. However, a proportion of patients require further investigation, due to the low negative predictive value (NPV). The objective of this study was to determine whether the assessment of short stature homeobox 2 (SHOX2) DNA methylation level in lymph node tissue obtained by EBUS-TBNA improves the accuracy of mediastinal staging. PATIENTS AND METHODS: EBUS-TBNA was carried out for suspicious lymph nodes of 154 patients. Negative or ambiguous histological results were confirmed by surgical means and clinical follow-up over 6 months. EBUS-TBNA was assessed on 80 positive and 85 negative classified lymph nodes and compared with the result of the SHOX2 DNA methylation real-time PCR analysis. Relative methylation measured by delta-delta cycle threshold (ΔΔCt) was used to classify the samples. Clinical performance of the EBUS-TBNA procedure with and without the additional SHOX2 assessment was calculated against the final classification according to the gold standard. RESULTS: Based on data from 105 patients, an average 80-fold increase in the SHOX2 methylation level was measured for positive compared with negative lymph nodes. SHOX2 results with a ΔΔCt value of <6.5 indicate positive lymph nodes. Applying this molecular analysis to EBUS-TBNA cases, not diagnosed by pathologic assessment, the sensitivity of staging was improved by 17%-99%. The NPV increased from 80% to 99%. CONCLUSIONS: The combination of EBUS-TBNA and SHOX2 methylation level strongly improves the assessment of the nodal status by identifying additional malignant lesions and confirming benign nodes and therefore avoiding invasive follow-up procedures.


Assuntos
Broncoscópios , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico , Proteínas de Homeodomínio/genética , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/genética , Linfonodos/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Metilação de DNA/genética , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/patologia , Linfonodos/metabolismo , Metástase Linfática/diagnóstico , Metástase Linfática/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias
8.
Zentralbl Chir ; 138(1): 111-6, 2013 Feb.
Artigo em Alemão | MEDLINE | ID: mdl-23450402

RESUMO

Tracheobronchial injuries are rare events but often life-threatening. A great expertise in the field of thoracic surgery is necessary to manage these events. The aetiology of the injury - traumatic or iatrogenic - allows immediate considerations about the pattern of damage. The flexible or rigid bronchoscopy is the central diagnostic procedure to classify the injury. Whereas iatrogenic tracheal lacerations can be treated by conservative or operative means depending on severity, traumatic injuries of the tracheo-bronchial system need technically challenging operative reconstructions in almost every case.


Assuntos
Brônquios/lesões , Broncoscopia/métodos , Emergências , Traumatismos Torácicos/cirurgia , Traqueia/lesões , Ferimentos não Penetrantes/cirurgia , Brônquios/cirurgia , Broncoscopia/efeitos adversos , Drenagem/métodos , Esôfago/lesões , Esôfago/cirurgia , Humanos , Doença Iatrogênica , Intubação Intratraqueal/efeitos adversos , Traumatismo Múltiplo/diagnóstico , Traumatismo Múltiplo/cirurgia , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/cirurgia , Ruptura , Esternotomia/métodos , Traumatismos Torácicos/diagnóstico , Traumatismos Torácicos/etiologia , Traqueia/cirurgia , Estenose Traqueal/diagnóstico , Estenose Traqueal/cirurgia , Traqueostomia/métodos , Ferimentos não Penetrantes/diagnóstico , Ferimentos não Penetrantes/etiologia , Ferimentos Penetrantes/cirurgia
9.
J Evol Biol ; 26(4): 832-42, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23516960

RESUMO

Models for the evolution of cannibalism highlight the importance of asymmetries between individuals in initiating cannibalistic attacks. Studies may include measures of body size but typically group individuals into size/age classes or compare populations. Such broad comparisons may obscure the details of interactions that ultimately determine how socially contingent characteristics evolve. We propose that understanding cannibalism is facilitated by using an interacting phenotypes perspective that includes the influences of the phenotype of a social partner on the behaviour of a focal individual and focuses on variation in individual pairwise interactions. We investigated how relative body size, a composite trait between a focal individual and its social partner, and the sex of the partners influenced precannibalistic aggression in the endangered Socorro isopod, Thermosphaeroma thermophilum. We also investigated whether differences in mating interest among males and females influenced cannibalism in mixed sex pairs. We studied these questions in three populations that differ markedly in range of body size and opportunities for interactions among individuals. We found that relative body size influences the probability of and latency to attack. We observed differences in the likelihood of and latency to attack based on both an individual's sex and the sex of its partner but found no evidence of sexual conflict. The instigation of precannibalistic aggression in these isopods is therefore a property of both an individual and its social partner. Our results suggest that interacting phenotype models would be improved by incorporating a new conditional ψ, which describes the strength of a social partner's influence on focal behaviour.


Assuntos
Canibalismo , Espécies em Perigo de Extinção , Isópodes/fisiologia , Comportamento Social , Agressão/fisiologia , Animais , Tamanho Corporal , Feminino , Masculino , Fenótipo , Fatores Sexuais , Especificidade da Espécie
10.
Eur J Cancer ; 49(9): 2107-15, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23510803

RESUMO

BACKGROUND: Radiation dose escalation within definitive radiochemotherapy (RTx/CTx) was not successful for stage III non-small cell lung cancer (NSCLC) using conventional fractionation (CF). Accelerated-hyperfractionation (AHF) counteracts tumour cell repopulation. In this observational study, the effects of neoadjuvant RTx/CTx using AHF or CF were studied by histopathology and using the survival end-point. METHODS: Data from all consecutive lung cancer patients treated with neoadjuvant RTx/CTx and thoracotomy between 08/2000 and 06/2012 were analysed. Patients received induction chemotherapy (cisplatin-doublets) followed by concurrent RTx/CTx using AHF (45 Gy/1.5 Gy bid) or CF-RTx (46 Gy/2 Gy qd). For estimating the AHF versus CF treatment effects, multivariate analysis (MA), propensity score weighting (PS), and instrumental variable analysis (IV) were used. FINDINGS: 239 patients were treated, median age 58 (34-78)years, stage II/IIIA/B: 19/88/132, squamous cell/adenocarcinomas/other: 98/107/34; AHF/CF-RTx 112/127 patients. No significant differences between both groups, in tumour related factors (age, gender, Charlson comorbiditiy score, lactate dehydrogenase (LDH), haemoglobin, stage, histopathology and grading), existed. Crude rates of pathologic complete responses (pCR) in AHF and CF groups were 37% and 24% respectively. The dose fractionation effect on pCR was significant (p ⩽ 0.006, PS and IV analyses). There was a significant dependence of pCR on biologically effective dose. pCR also depended on treatment time (MA, p = 0.04; PS, p = 0.0004). Median treatment time was 22 d or 31 d using AHF or CF (p<0.0001), respectively. Adenocarcinomas had lower pCR rates in comparison to other histologies. Five-year survival of patients with pCR was 65%, independent of the fractionation. INTERPRETATION: This large monoinstitutional analysis demonstrates an increased effect of AHF on pCR of lung cancer which modifies overall survival.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/terapia , Quimiorradioterapia Adjuvante/métodos , Neoplasias Pulmonares/terapia , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Quimiorradioterapia Adjuvante/mortalidade , Cisplatino/administração & dosagem , Terapia Combinada , Fracionamento da Dose de Radiação , Etoposídeo/administração & dosagem , Feminino , Humanos , Quimioterapia de Indução/métodos , Neoplasias Pulmonares/mortalidade , Masculino , Pessoa de Meia-Idade , Pontuação de Propensão , Resultado do Tratamento
11.
Pathologe ; 34(4): 338-42, 2013 Jul.
Artigo em Alemão | MEDLINE | ID: mdl-23263441

RESUMO

Benign epithelial tumors of the tracheobronchial system and the lungs are exceedingly rare. These entities encompass squamous and glandular papillomas (as well as their mixed forms) and adenomas (alveolar adenoma, papillary adenoma, salivary gland-like pleomorphic and mucinous adenomas and mucinous cystadenomas). These tumors are considered to be biologically benign neoplasms; however, they can pose considerable diagnostic difficulties, especially during frozen section evaluation, as they can mimic malignant tumors and in particular they can resemble well differentiated papillary adenocarcinomas. As a result of the extreme rarity of these tumors only a few descriptive diagnostic series exist and a systematic investigation including molecular data does not exist. This article presents the case of a 64-year-old patient with a glandular papilloma of the right main bronchus including the immunohistochemical and molecular work-up as well as a review of the current literature.


Assuntos
Neoplasias Brônquicas/genética , Neoplasias Brônquicas/patologia , Éxons/genética , Mutação/genética , Papiloma/genética , Proteínas Proto-Oncogênicas/genética , Proteínas ras/genética , Adenocarcinoma Papilar/genética , Adenocarcinoma Papilar/patologia , Substituição de Aminoácidos/genética , Asparagina/genética , Brônquios/patologia , Brônquios/cirurgia , Neoplasias Brônquicas/cirurgia , Broncoscopia , Diagnóstico Diferencial , Receptores ErbB/genética , Feminino , Secções Congeladas , Glicina/genética , Humanos , Pessoa de Meia-Idade , Papiloma/patologia , Papiloma/cirurgia , Pneumonectomia , Proteínas Proto-Oncogênicas p21(ras) , Análise de Sequência de DNA , Proteína Supressora de Tumor p53/genética
12.
Br J Cancer ; 107(5): 823-30, 2012 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-22836512

RESUMO

BACKGROUND: Tubulin-binding agents (TBAs) are effective in non-small cell lung cancer (NSCLC) treatment. Both ßIII- and ßV-tubulins are expressed by cancer cells and may lead to resistance against TBAs. METHODS: Pre-treatment samples from 65 locally advanced or oligometastatic NSCLC patients, who underwent uniform induction chemotherapy with paclitaxel and platinum followed by radiochemotherapy with vinorelbine and platinum were retrospectively analysed by immunohistochemistry. Protein expression of ßIII- and ßV-tubulin was morphometrically quantified. RESULTS: Median pre-treatment H-score for ßIII-tubulin was 110 (range: 0-290), and 160 for ßV-tubulin (range: 0-290). Low ßIII-tubulin expression was associated with improved overall survival (OS) (P=0.0127, hazard ratio (HR): 0.328). An association between high ßV-tubulin expression and prolonged progression-free survival (PFS, median 19.2 vs 9.4 months in high vs low expressors; P=0.0315, HR: 1.899) was found. Further, high ßV-tubulin expression was associated with objective response (median H-score 172.5 for CR+PR vs 120 for SD+PD patients, P=0.0104) or disease control following induction chemotherapy (170 for CR+PR+SD vs 100 for PD patients, P=0.0081), but not radiochemotherapy. CONCLUSION: Expression of ßV-tubulin was associated with treatment response and PFS following paclitaxel-based chemotherapy of locally advanced and oligometastatic NSCLC patients. Prolonged OS was associated with low levels of ßIII-tubulin. Prospective evaluation of ßIII/ßV-tubulin expression in NSCLC is warranted.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , Tubulina (Proteína)/biossíntese , Adulto , Idoso , Hidrocarbonetos Aromáticos com Pontes/administração & dosagem , Carcinoma Pulmonar de Células não Pequenas/patologia , Estudos de Coortes , Intervalo Livre de Doença , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Taxoides/administração & dosagem , Transfecção , Resultado do Tratamento , Tubulina (Proteína)/genética
13.
Zentralbl Chir ; 137(3): 242-7, 2012 Jun.
Artigo em Alemão | MEDLINE | ID: mdl-22711324

RESUMO

Today several methods for invasive mediastinal staging of lung cancer are available. Whereas mediastinoscopy and anterior mediastinotomy had been the gold standard in every situation several years ago, today EBUS-TBNA has been developed as an alternative to mediastinoscopy concerning the status of lymph node positions 2 L / R, 4 L / R and 7. Actually mediastinoscopy is accepted as the gold standard only in special situations such as negative cytology of suspicious lymph nodes after EBUS-TBNA and mediastinal evaluation after neoadjuvant treatment.


Assuntos
Neoplasias Pulmonares/patologia , Mediastinoscopia/métodos , Terapia Combinada , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/mortalidade , Excisão de Linfonodo/métodos , Terapia Neoadjuvante , Invasividade Neoplásica , Estadiamento de Neoplasias , Prognóstico , Taxa de Sobrevida
14.
Ann Oncol ; 23(10): 2649-55, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22547539

RESUMO

BACKGROUND: Although resecting colorectal cancer (CRC) pulmonary metastasis is associated with long-term survival, identification of prognostic groups is needed for future randomized trials, and construction of a lung metastasectomy prognostic model (LMPM) is warranted. PATIENTS AND METHODS: We searched the PubMed database for retrospective studies evaluating prognostic factors following resecting CRC lung metastasis. Individual patient data were analyzed. Independent prognostic factors were used to construct an LMPM. RESULTS: Between 1983 and 2008, 1112 metastasectomies were carried out on 927 patients included in eight studies. Five-year survival rate was 54.3% following the first lung resection. Multivariate analysis identified three independently poor prognostic factors: pre-thoracotomy carcinoembryonic antigen ≥5 ng/ml, disease-free interval <36 months, and more than one metastatic lesion. Patients with good-, intermediate-, and high-risk groups according to the LMPM had a 5-year survival of 68.2%, 46.4%, and 26.1%, respectively (P < 0.001). Perioperative chemotherapy and previously resected liver metastasis had no influence on survival. CONCLUSIONS: The low- and intermediate-risk groups have a good chance of long-term survival following metastasectomy. However, more studies are needed to investigate whether surgery offers any advantage over systemic therapy for the poor-risk group.


Assuntos
Neoplasias Colorretais/patologia , Neoplasias Pulmonares/secundário , Feminino , Humanos , Masculino , Modelos Biológicos , Prognóstico
15.
Thorac Cardiovasc Surg ; 60(2): 93-100, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21695673

RESUMO

BACKGROUND: Segmental resection in stage I non-small cell lung cancer (NSCLC) has been well described and is considered to have similar survival rates as lobectomy but with increased rates of local tumour recurrence due to inadequate parenchymal margins. In consequence, today segmentectomy is only performed when the tumour is smaller than 2 cm. METHODS: Three-dimensional reconstructions from 11 thin-slice CT scans of bronchopulmonary segments were generated, and virtual spherical tumours were placed over the segments, respecting all segmental borders. As a next step, virtual parenchymal safety margins of 2 cm and 3 cm were subtracted and the size of the remaining tumour calculated. RESULTS: The maximum tumour diameters with a 30-mm parenchymal safety margin ranged from 26.1 mm in right-sided segments 7 + 8 to 59.8 mm in the left apical segments 1-3. CONCLUSIONS: Using a three-dimensional reconstruction of lung CT scans, we demonstrated that segmentectomy or resection of segmental groups should be feasible with adequate margins, even for larger tumours in selected cases.


Assuntos
Brônquios/cirurgia , Carcinoma Pulmonar de Células não Pequenas/cirurgia , Simulação por Computador , Neoplasias Pulmonares/cirurgia , Recidiva Local de Neoplasia/prevenção & controle , Pneumonectomia , Brônquios/patologia , Carcinoma Pulmonar de Células não Pequenas/diagnóstico por imagem , Carcinoma Pulmonar de Células não Pequenas/patologia , Humanos , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Estadiamento de Neoplasias , Neoplasia Residual , Seleção de Pacientes , Valor Preditivo dos Testes , Interpretação de Imagem Radiográfica Assistida por Computador , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Carga Tumoral
16.
Bull Entomol Res ; 93(3): 179-85, 2003 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12762859

RESUMO

Cacopsylla pyricola (Förster) is one of the most important pests of pear in North America, where several native predators have been considered for integrated pest management (IPM) programmes. Two molecular markers of 271 and 188 bp were developed from C. pyricola cytochrome oxidase I (COI) fragments, in order to study the detection of this species in the gut of arthropod predators. Primer sensitivity and the detection period for pear psylla remains in the guts of Anthocoris tomentosus Pericart were determined. The sensitivity threshold was defined at 10-5 dilution of a C. pyricola fifth-instar nymph in all samples. Predator adults were evaluated immediately after ingestion of one to five C. pyricola nymphs (t = 0) and after 2, 4, 6, 8, 16, 24 and 32 h. Detection of the presence of C. pyricola DNA always lasted longer using the shorter fragment and was observed after 32 h of digestion using both markers. The primers amplifying the 188 bp fragment amplified all four psyllid species tested, whereas the primers designed to amplify the 271 bp fragment did so exclusively for C. pyricola and its close relative, Cacopsylla pyri (Linnaeus). Both primers failed to amplify DNA from representative species of the Coccinellidae, Chrysopidae, Hemerobiidae, Anthocoridae, Miridae, Salticidae, Aphididae, Tetranychidae and the Tortricidae, suggesting their suitability for general trophic studies.


Assuntos
Aracnídeos/fisiologia , Complexo IV da Cadeia de Transporte de Elétrons/genética , Hemípteros/genética , Insetos/fisiologia , Controle Biológico de Vetores , Animais , DNA Mitocondrial/isolamento & purificação , Feminino , Frutas/parasitologia , Amplificação de Genes , Marcadores Genéticos , Hemípteros/enzimologia , Comportamento Predatório , Análise de Sequência de DNA
17.
Nature ; 421(6918): 54-7, 2003 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-12511951

RESUMO

Research on new materials for organic electroluminescence has recently focused strongly on phosphorescent emitters, with the aim of increasing the emission efficiency and stability. Here we report the fabrication of a simple electroluminescent device, based on a semiconducting polymer combined with a phosphorescent complex, that shows fully reversible voltage-dependent switching between green and red light emission. The active material is made of a polyphenylenevinylene (PPV) derivative molecularly doped with a homogeneously dispersed dinuclear ruthenium complex, which fulfils the dual roles of triplet emitter and electron transfer mediator. At forward bias (+4 V), the excited state of the ruthenium compound is populated, and the characteristic red emission of the complex is observed. On reversing the bias (-4 V), the lowest excited singlet state of the polymer host is populated, with subsequent emission of green light. The mechanism for the formation of the excited state of the PPV derivative involves the ruthenium dinuclear complex in a stepwise electron transfer process that finally leads to efficient charge recombination reaction on the polymer.

18.
J Econ Entomol ; 94(2): 373-80, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11332828

RESUMO

Two species of leafrollers, Argyrotaenia citrana (Fernald) and Pandemis pyrusana Kearfott, represent serious obstacles to the implementation of mating disruption for control of codling moth in coastal California apple orchards. Larval and adult densities of A. citrana and P. pyrusana and subsequent fruit damage were compared under different codling moth control treatments. Leafroller larval counts and levels of fruit damage were significantly higher in most plots that were untreated or treated only with codling moth pheromone. Leafroller fruit damage levels in these plots were commonly between 10 and 15% at harvest. As summer larval counts were good predictors of fruit damage levels, larval sampling could be a useful tool for predicting leafroller outbreaks. Use of pheromone trapping for A. citrana to detect localized outbreaks within an orchard was not useful and failed to correlate with larval numbers, whereas adult monitoring for P. pyrusana appears more promising. Efforts to implement a codling moth mating-disruption program in California must include changes in strategies for monitoring and controlling leafroller species.


Assuntos
Mariposas/fisiologia , Controle Biológico de Vetores/métodos , Rosales , Comportamento Sexual Animal , Animais , California , Feminino , Masculino , Densidade Demográfica
19.
Chirurg ; 72(1): 86-8, 2001 Jan.
Artigo em Alemão | MEDLINE | ID: mdl-11225463

RESUMO

Pulmonary blastoma is a rare non-small-cell lung cancer. In a statistically significant proportion of cases it is combined with cystic lung disease in children. The biphasic pulmonary blastoma and the well-differentiated fetal adenocarcinoma are the most frequent manifestations of pulmonary blastoma. If metastatic disease is ruled out, surgical therapy should be guided by the principles for radical therapy of NSCLC. There are no sufficient recommendations yet for therapy of stage IIIb and IV (UICC 1997) disease.


Assuntos
Neoplasias Pulmonares/cirurgia , Blastoma Pulmonar/cirurgia , Idoso , Diagnóstico Diferencial , Humanos , Pulmão/patologia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Masculino , Estadiamento de Neoplasias , Pneumonectomia , Blastoma Pulmonar/diagnóstico por imagem , Blastoma Pulmonar/patologia , Tomografia Computadorizada por Raios X
20.
J Econ Entomol ; 93(3): 955-62, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10902355

RESUMO

Resistance to several classes of insecticides was correlated with azinphosmethyl resistance in codling moth, Cydia pomonella (L.), in California. In tests of laboratory and field populations, cross-resistance was positively correlated with azinphosmethyl and two organophosphates (diazinon, phosmet), a carbamate (carbaryl), a chlorinated hydrocarbon (DDT), and two pyrethroids (esfenvalerate and fenpropathrin). Additionally, negatively correlated cross-resistance was identified between azinphosmethyl and two other organophosphates, chlorpyrifos and methyl parathion. Patterns of resistance observed in laboratory colonies were confirmed with field bioassays. In bioassays of field populations, azinphosmethyl resistance was observed to increase from 1991 to 1993, although levels of resistance remained < 13-fold. Because orchards with azinphosmethyl resistance have had difficulties with suppression of codling moth, and cross-resistance was found for all tested classes of insecticides, strategies for managing resistance will need to be developed so as to protect current and future control tactics. The two insecticides with negatively correlated cross-resistance are discussed as potential tools for resistance management.


Assuntos
Azinfos-Metil , Inseticidas , Mariposas , Animais , Bioensaio , Feminino , Controle de Insetos/métodos , Resistência a Inseticidas , Masculino
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA