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1.
Sci China Life Sci ; 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36115892

RESUMO

The human retina serves as a light detector and signals transmission tissue. Advanced insights into retinal disease mechanisms and therapeutic strategies require a deep understanding of healthy retina molecular events. Here, we sequenced the mRNA of over 0.6 million single cells from human retinas across six regions at nine different ages. Sixty cell sub-types have been identified from the human mature retinas with unique markers. We revealed regional and age differences of gene expression profiles within the human retina. Cell-cell interaction analysis indicated a rich synaptic connection within the retinal cells. Gene expression regulon analysis revealed the specific expression of transcription factors and their regulated genes in human retina cell types. Some of the gene's expression, such as DKK3, are elevated in aged retinas. A further functional investigation suggested that over expression of DKK3 could impact mitochondrial stability. Overall, decoding the molecular dynamic architecture of the human retina improves our understanding of the vision system.

2.
Front Immunol ; 13: 944812, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36032124

RESUMO

Lung adenosquamous carcinoma (ASC) is an uncommon histological subtype. We aimed to characterize the tumor immune microenvironment (TIME) in lung ASC and estimate patient response to immune checkpoint inhibitors (ICIs), which have never been systematically investigated. In cohort I, we collected 30 ASCs from a single center for analysis of TIME characteristics, including immuno-phenotyping, tumor mutation burden (TMB), T-cell receptor (TCR) repertoires, tumor-infiltrating lymphocytes (TILs), and immune checkpoint expression. Twenty-two (73.3%) patients were EGFR-positive. The TIME was defined by immune-excluded (60%) and immune-desert phenotype (40%). Strikingly, programmed cell death-ligand 1 (PD-L1) and programmed cell death-1 (PD-1) were predominantly expressed in squamous cell carcinoma components (SCCCs) versus adenocarcinoma components (ACCs), where enhanced CD4+ FOXP3+ regulatory T cell and attenuated CD57+ natural killer cell infiltration were present, consistent with a landscape of fewer innate immune cells, more immunosuppressive cells. SCCCs had higher TMB, higher TCR clonality, and lower TCR diversity than ACC. In cohort III, the efficacy of ICI-based therapy was estimated using a real-world data of 46 ASCs from 11 centers. Majority of 46 patients were driver genes negative and unknown mutation status, 18 (39%) and 18 (39%), respectively. The overall objective response rate of 28%, median progression-free survival of 6.0 months (95% confidence interval [CI] 4.3-7.7), and median overall survival of 24.7 months (95% CI 7.2-42.2) were observed in the ICI-based treatment. This work ascertains suppressive TIME in lung ASC and genetic and immuno-heterogeneity between ACCs and SCCCs. Lung ASC patients have a moderate response to ICI-based immunotherapy.


Assuntos
Adenocarcinoma , Carcinoma Adenoescamoso , Carcinoma Pulmonar de Células não Pequenas , Carcinoma de Células Escamosas , Neoplasias Pulmonares , Antígeno B7-H1 , Humanos , Imunoterapia , Pulmão , Receptores de Antígenos de Linfócitos T , Microambiente Tumoral
3.
Nanoscale ; 14(34): 12447-12454, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-35979926

RESUMO

Moiré superlattices offer a fascinating platform for designing the properties of optical excitons. The moiré pattern can generate an ordered exciton array in space, making it possible for topological excitons and quantum emitters. Recently, evidence of moiré excitons in the twisted heterostructures of TMDs has been widely reported. However, to date, the capture and investigation of moiré excitons in the twisted homostructure (T-HS) remain elusive. Here, we report the observation of moiré excitons in the WS2/WS2 T-HS with a twist angle of about 1.5°. The PL spectrum of the T-HS region shows many small peaks with nearly constant peak spacing, which is attributed to the reconstructed moiré potential generated by the reconstructed moiré pattern to confine the intralayer excitons, thereby forming an ordered moiré exciton array. Furthermore, we have studied the influence of temperature and laser power on the moiré excitons as well as the valley polarization of the moiré excitons. Our results provide a promising prospect for further exploration of artificial excitonic crystals and quantum emitters of TMD moiré patterns.

4.
Biomed Res Int ; 2022: 4541571, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35795311

RESUMO

ZC3H12C is an important member of the CCCH-zinc finger protein family and is mainly involved in host immune and inflammatory diseases. However, its abnormal expression and prognostic value in cancer have not yet been established. Through comparative analysis of the Cancer Genome Atlas (TCGA) database, we found that ZC3H12C is the most relevant to the prognosis, grade, and stage of renal clear cell carcinoma (ccRCC) across 33 cancers. With the help of patient transcription and clinical data from the TCGA and GEO (GSE53757, GSE36895) databases, we determined that in the immune environment of patients with ccRCC, ZC3H12C was clearly negatively correlated with Tregs and was significantly positively correlated with monocytes. In addition, protein phosphorylation and DNA methylation analysis also showed that ZC3H12C negatively regulates the role of cancer in ccRCC. Our research may provide new insights into ccRCC immunotherapy and bring forth novel biomarkers and therapeutic targets.


Assuntos
Carcinoma de Células Renais , Neoplasias Renais , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Renais/patologia , Humanos , Neoplasias Renais/patologia , Prognóstico
5.
Radiat Oncol ; 17(1): 127, 2022 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-35850908

RESUMO

BACKGROUND: Tumor-infiltrating lymphocytes (TILs), investigated using routine hematoxylin and eosin (H&E)-stained section slides (H&E-sTILs), provide a robust prognostic biomarker in various types of solid cancer. The purpose of the present study was to investigate the prognostic significance of H&E-sTILs in patients with small cell lung cancer (SCLC). METHODS: The clinical data of patients with SCLC who had been treated in our cancer center between January 2013 and October 2019 were collected and retrospectively reviewed. The H&E-sTILs were re-assessed by two experienced pathologists independently. H&E-sTILs that affected the overall survival (OS), progression free survival (PFS) and brain-metastasis free survival (BMFS) rates were explored using the Kaplan-Meier method, and the log-rank test was used to assess the differences. Multivariate analysis was subsequently performed using the Cox proportion hazards model. RESULTS: A total of 159 patients with SCLC who fulfilled the inclusion criteria were enrolled in the current study. The OS rates at 1, 2 and 3 years were 59.8, 28.6 and 19.8%, respectively, for the whole group. The 3-year OS, PFS and BMFS rates for the H&E-sTILs(+) and H&E-sTILs(-) groups were 25.1% cf. 5.1% (P = 0.030), 14.0% cf. 4.0% (P = 0.013), and 66.0% cf. 11.4% (P = 0.023), respectively. Multivariate analyses subsequently revealed that H&E-sTILs, clinical M stage, the cycles of chemotherapy and short-term response to thoracic radiotherapy were independent factors affecting OS, whereas H&E-sTILs, clinical N stage, clinical M stage and short-term response to chemotherapy were factors affecting PFS. The H&E-sTILs affected OS, PFS and BMFS simultaneously. CONCLUSIONS: The results of this retrospective study have shown that H&E-sTILs may be considered as a prognostic biomarker affecting the short-term response to treatment, and they are the one and only risk factor for BMFS. However, due to the limitations of the nature of the retrospective design and shortcomings in visually assessing the TILs based on the H&E-stained slides, further prospective studies are required to confirm these conclusions.


Assuntos
Neoplasias Pulmonares , Carcinoma de Pequenas Células do Pulmão , Biomarcadores/metabolismo , Humanos , Neoplasias Pulmonares/patologia , Linfócitos do Interstício Tumoral/patologia , Prognóstico , Estudos Retrospectivos , Carcinoma de Pequenas Células do Pulmão/patologia
6.
Zhongguo Gu Shang ; 35(6): 532-7, 2022 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-35730222

RESUMO

OBJECTIVE: To compare computed tomography (CT) measurement results of external deviation angle of patellar tendon and tibia tubercle-trochlea groove (TT-TG), as well as the diagnostic ability and pathology in recurrent patellar dislocation threshold. METHODS: From January 2015 to March 2020, 46 patients with recurrent patella dislocation and 112 patients with non-patella dislocation were retrospectively analyzed. Forty-six patients with recurrent patella dislocation were divived into 2 groups according to TT-TG value, 11 patients with patellar dislocation with TT-TG≥20 mm(group A), including 7 males and 4 females, aged from 16 to 27 years old with an average of(21.00±3.98) years old; 35 patients with patellar dislocation with TT-TG<20 mm(group B), including 14 males and 21 females, aged from 16 to 37 years old with an average of(22.83±6.09) years old. While 112 patients with non-patella dislocation(group C) included 63 males and 49 females, aged 16 to 36 years old with an average of(22.87±5.69) years old. The measurement data of external deviation angle of patellar tendon and TT-TG value among three groups were compared. Spearman analysis was used to analyze correlation among them. Intraclass correlation coefficient (ICC) was used to determine repeatability within group. Receiver operating characteristic (ROC) area under the curve was used to evaluate diagnostic ability of parameters, and calculate osteotomy parameters of external deviation angle of patellar tendon, as well as external deviation angle of patellar tendon and TT-TG value in the diagnosis of recurrent patella diagnostic parameters of dislocation. RESULTS: External deviation angle of patellar tendon in group A, B and C was (22.04±3.09)°, (17.20±4.43)°and (10.22±3.45)° respectively;while TT-TG value was(21.15±0.71) mm, (15.97±2.69) mm and (11.12±3.77) mm, there were significance among three groups (P<0.01), and had difference between group A and B(P<0.01). There was strong positive correlation between external deviation angle of patellar tendon and TT-TG value (r=0.735, P<0.000 1). The intra-group ICC value(0.980, 0.982) of external deviation angle of patellar tendon in group A and B have better repeatability than TT-TG value (0.594, 0.775). The external deviation angle of patellar tendon in group C(0.956) and repeatability of TT-TG value(0.906) was very good. In the diagnosis of recurrent patellar dislocation, the area under ROC curve of external deviation angle of patellar tendon (0.916) was greater than TT-TG value(0.886), and diagnostic parameters were 13.84°and 14.69 mm, respectively;in tibial osteotomy, the area under ROC curve of external deviation angle of patellar tendon was 0.821, and osteotomy parameter was 20.15°. CONCLUSION: CT imaging could reliably measure external deviation angle of patellar tendon.There is a strong positive correlation between external deviation angle of patellar tendon and value of TT-TG, which could be used to determine pathological state of recurrent patellar dislocation, and external deviation angle of patellar tendon is superior to the TT-TG value in the diagnosis of recurrent patellar dislocation. The external deviation angle of patellar tendon could also be used to guide the formulation of the tibial osteotomy plan for recurrent patellar dislocation.


Assuntos
Instabilidade Articular , Luxação Patelar , Ligamento Patelar , Articulação Patelofemoral , Adolescente , Adulto , Feminino , Humanos , Masculino , Patela/cirurgia , Luxação Patelar/diagnóstico por imagem , Ligamento Patelar/diagnóstico por imagem , Estudos Retrospectivos , Tíbia/cirurgia , Adulto Jovem
7.
Med Sci Monit ; 28: e937005, 2022 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-35726168

RESUMO

BACKGROUND The Integrated Liver Inflammatory Score (ILIS), which includes 5 serum indicators (albumin, bilirubin, neutrophil count, alpha-fetoprotein [AFP], and alkaline phosphatase [ALP]), is a novel inflammation-based predictive model associated with poor survival in hepatocellular carcinoma (HCC) patients. Our study aimed to assess the prognostic value of ILIS in HCC patients undergoing radical hepatectomy and establish a nomogram and artificial neural network based on their ILIS scores. MATERIAL AND METHODS This multicenter retrospective study included patients from 2 institutions from 2007 to 2017. Independent risk factors associated with Recurrence-free survival (RFS) and overall survival (OS) were identified through univariate and multifactor analysis in the training and validation groups, respectively. Afterward, column line graphs and artificial neural networks (ANN) were constructed and validated using the validation group. RESULTS A total of 432 patients were included in this study (275 in the training group and 157 in the validation group). In both cohorts, ILIS was correlated with pathological features such as tumor size, degree of differentiation, Child-Pugh class classification, and BCLC staging. Moreover, ILIS was identified as an independent risk factor for OS. ILIS-based nomograms and artificial neural networks also showed the prognostic value of ILIS. CONCLUSIONS Preoperative ILIS is an independent and effective predictor of prognosis in HCC patients treated with radical hepatectomy, as shown by the fact that higher ILIS are associated with worse patient prognosis. We have also established nomograms and ANNs that predict HCC prognosis with high accuracy.


Assuntos
Carcinoma Hepatocelular , Hepatite , Neoplasias Hepáticas , Carcinoma Hepatocelular/patologia , Hepatectomia/métodos , Humanos , Neoplasias Hepáticas/patologia , Prognóstico , Estudos Retrospectivos
8.
Light Sci Appl ; 11(1): 166, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35650176

RESUMO

Recent advances in twisted van der Waals heterostructure superlattices have emerged as a powerful and attractive platform for exploring novel condensed matter physics due to the interplay between the moiré potential and Coulomb interactions. The moiré superlattices act as a periodic confinement potential in space to capture interlayer excitons (IXs), resulting in moiré exciton arrays, which provide opportunities for quantum emitters and many-body physics. The observation of moiré IXs in twisted transition-metal dichalcogenide (TMD) heterostructures has recently been widely reported. However, the capture and study of the moiré intralayer excitons based on TMD twisted homobilayer (T-HB) remain elusive. Here, we report the observation of moiré intralayer excitons in a WSe2/WSe2 T-HB with a small twist angle by measuring PL spectrum. The multiple split peaks with an energy range of 1.55-1.73 eV are different from that of the monolayer WSe2 exciton peaks. The split peaks were caused by the trapping of intralayer excitons via the moiré potential. The confinement effect of the moiré potential on the moiré intralayer excitons was further demonstrated by the changing of temperature, laser power, and valley polarization. Our findings provide a new avenue for exploring new correlated quantum phenomena and their applications.

9.
Chem Sci ; 13(17): 4915-4921, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35655878

RESUMO

Synthetic foldamers with helical conformation are widely seen, but controllable interconversion amongst different geometries (helical structure and sense) is challenging. Here, a family of oligourea (tetra-, penta-, and hexa-) ligands bearing stereocenters at both ends are designed and shown to switch between single and double helices with concomitant inversion of helical senses upon anion coordination. The tetraurea ligand forms a right-handed single helix upon chloride anion (Cl-) binding and is converted into a left-handed double helix when phosphate anion (PO4 3-) is coordinated. The helical senses of the single and double helices are opposite, and the conversion is further found to be dependent on the stoichiometry of the ligand and phosphate anion. In contrast, only a single helix is formed for the hexaurea ligand with the phosphate anion. This distinction is attributed to the fact that the characteristic phosphate anion coordination geometry is satisfied by six urea moieties with twelve H-bonds. Our study revealed unusual single-double helix interconversion accompanied by unexpected chiroptical switching of helical senses.

10.
Mech Ageing Dev ; 205: 111688, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35728631

RESUMO

Osteoarthritis (OA) is an age-related chronic degenerative disease, and chondrocyte senescence has been established to play an important role in the pathological process. There is ample evidence to suggest that lipid metabolism plays an important role in the aging process. However, the effect of lipid metabolism on chondrocyte senescence and OA remains unclear. Accordingly, we constructed a TBHP-induced senescent chondrocytes model and a destabilization of the medial meniscus (DMM) mouse model. We found that lipid accumulation and fatty acid oxidation were enhanced in senescent chondrocytes. Interestingly, carnitine palmitoyltransferase 1A (Cpt1a), the rate-limiting enzyme for fatty acid oxidation, was highly expressed in senescent chondrocytes and murine knee cartilage tissue. Suppressing Cpt1a expression using siRNA or Etomoxir, an inhibitor of Cpt1a, could attenuate oxidative stress-induced premature senescence and OA phenotype of primary murine chondrocytes, decrease cellular ROS levels, restore mitochondrial function, and maintain mitochondrial homeostasis via activating mitophagy. In vivo, pharmacological inhibition of Cpt1a by Etomoxir attenuated cartilage destruction, relieved joint space narrowing and osteophyte formation in the DMM mouse model. Overall, these findings suggested that knockdown of Cpt1a alleviated chondrocyte senescence by regulating mitochondrial dysfunction and promoting mitophagy, providing a new therapeutic strategy and target for OA treatment.


Assuntos
Cartilagem Articular , Osteoartrite , Animais , Cartilagem Articular/metabolismo , Cartilagem Articular/patologia , Senescência Celular/fisiologia , Condrócitos/metabolismo , Ácidos Graxos/metabolismo , Camundongos , Mitocôndrias/metabolismo , Mitofagia , Osteoartrite/metabolismo , Estresse Oxidativo/fisiologia
11.
Genomics ; 114(4): 110409, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35714827

RESUMO

Increasing evidences point to the potential role of microRNAs (miRNAs) in muscle growth and development in animals. However, knowledge on the identity of miRNAs and their targets in molluscs remains largely unknown. Scallops have one large adductor muscle, composed of fast (striated) and slow (smooth) muscle types, which display great differences in muscle fibers, meat quality, cell types and molecular components. In the present study, we performed a comprehensive investigation of miRNA transcriptomes in fast and slow adductor muscles of Yesso scallop Patinopecten yessoensis. As a result, 47 differentially expressed miRNAs representing ten miRNA families were identified between the striated and smooth adductor muscles. The KEGG enrichment analysis of their target genes were mainly associated with amino acid metabolism, energy metabolism and glycan biosynthesis. The target genes of miR-133 and miR-71 were validated by the dual-luciferase reporter assays and miRNA antagomir treatment in vivo. The identification and functional validation of these different miRNAs in scallops will greatly help our understanding of miRNA regulatory mechanism that achieves the unique muscle phenotypes in scallops. The present findings provide the direct evidences for muscle-specific miRNAs involved in muscle growth and differentiation in molluscs.


Assuntos
MicroRNAs , Pectinidae , Animais , MicroRNAs/genética , MicroRNAs/metabolismo , Músculo Esquelético , Pectinidae/genética , Pectinidae/metabolismo , Transcriptoma
12.
Cell Prolif ; 55(9): e13285, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35716032

RESUMO

Osteoarthritis (OA) is a common joint disease featured by the deterioration of articular cartilage and chondrocyte death. Emerging evidence has indicated that circular RNAs (circRNAs) play an essential role in OA progress. Here, we found that the expression of circHIPK3 was significantly decreased in human and mouse OA cartilage. Knocking down circHIPK3 increased apoptosis and intracellular ROS level in HC-a chondrocytes. We performed proteomic studies and identified that circHIPK3 regulated chondrocyte apoptosis through the mitochondrial pathway. Results of JC-1 staining and western blot further confirmed that mitochondrial outer membrane permeabilization was promoted in HC-a chondrocytes transfected by circHIPK3 siRNA. In terms of mechanism, we showed that PON2 functioned as a potential target of circHIPK3 to regulate chondrocyte apoptosis. Moreover, we revealed that circHIPK3 interacted with miR-30a-3p to regulate PON2 expression in chondrocytes. Taken together, our findings suggested that circHIPK3 regulated chondrocyte apoptosis by mitochondrial pathway, and targeting the circHIPK3/miR-30a-3p/PON2 axis might be a potential strategy for OA treatment.


Assuntos
Cartilagem Articular , Peptídeos e Proteínas de Sinalização Intracelular/genética , MicroRNAs , Osteoartrite , Proteínas Serina-Treonina Quinases/genética , Animais , Apoptose , Arildialquilfosfatase/metabolismo , Cartilagem Articular/metabolismo , Condrócitos/metabolismo , Humanos , Camundongos , MicroRNAs/metabolismo , Osteoartrite/genética , Osteoartrite/metabolismo , Proteômica
13.
J Biomech ; 139: 111120, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35588559

RESUMO

The objective of this study was to quantitatively analyze the effect of lumbar spinal muscle atrophy on the compressive (perpendicular to the upper surface of the disc) and shear (parallel to the upper surface of the disc in the anterior-posterior direction) forces change on lumbar intervertebral discs using a full body musculoskeletal modeling approach. Muscles atrophy was modeled with reduction of the functional cross-sectional area (FCSA) of the muscles. Compressive and shear forces under two levels of lumbar muscle atrophy (20% and 40%) at eight daily postures (lying on back, seating slouched, seating straight, standing, standing flexed (36°), standing lift a 20 kg weight close to chest, standing lift a 20 kg weight flexed (38°), and standing lift a 20 kg weight with arm stretched) were analyzed. There was small increase in compressive forces on lumbar discs with muscle atrophy at most postures except lying and sitting straight. The maximum increase of compressive forces on lumbar discs were 23 N (6%), 28 N (5%), 34 N (2%), 71 N (6%), 89 N (4%), and 190 N (10%) with 20% atrophy, and 66 N (19%), 77 N (12%), 98 N (6%), 169 N (14%), 256 N (12%), 501 N (24%) with 40% atrophy at seating slouched, standing, standing flexed, standing lift close, standing lift flexed, and standing stretched arm, respectively. The shear force did not change significantly on lumbar discs with muscle atrophy. This study is important for understanding the biomechanical mechanisms of how lumbar muscle atrophy may affect the lumbar IVD health.


Assuntos
Disco Intervertebral , Vértebras Lombares , Atrofia Muscular Espinal , Suporte de Carga , Fenômenos Biomecânicos , Humanos , Disco Intervertebral/fisiologia , Vértebras Lombares/fisiologia , Atrofia Muscular Espinal/fisiopatologia , Postura/fisiologia , Suporte de Carga/fisiologia
14.
Cell Commun Signal ; 20(1): 75, 2022 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-35637489

RESUMO

BACKGROUND: The transforming growth factor-beta (TGF-ß) signaling pathway is an important pathway associated with the pathogenesis of osteoarthritis (OA). This study was to investigate the involvement of circRNAs in the TGF-ß signaling pathway. METHODS: Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) assay were used to detect the proliferation of primary mouse chondrocytes (PMCs). RNA-sequencing together with bioinformatics analysis were used to systematically clarify TGF-ß1 induced alternations of circRNAs in PMCs. The regulatory and functional role of circPhf21a was examined in PMCs. Downstream targets of circPhf21a were explored by RNA-sequencing after overexpression of circPhf21a and verified by RT-qPCR in PMCs. Finally, the role and mechanism of circPhf21a in OA were explored in mouse models. RESULTS: We found that TGF-ß1 promoted the proliferation of PMCs. Meanwhile, RT-qPCR and western blotting indicated that TGF-ß1 promoted extracellular matrix (ECM) anabolism. RNA-sequencing revealed that a total of 36 circRNAs were differentially expressed between PMCs treated with and without TGF-ß1. Of these, circPhf21a was significantly decreased by TGF-ß1. Furthermore, circPhf21a knockdown promoted the proliferation and ECM synthesis of PMCs, whereas overexpression of circPhf21a showed the opposite effects. Mechanically, the expression profiles of the mRNAs revealed that Vegfa may be the target of circPhf21a. Additionally, we found that circPhf21a was significantly upregulated in the mouse OA model, and inhibition of circPhf21a significantly relieved the progression of OA. CONCLUSIONS: Our results found that TGF-ß1 promoted the proliferation and ECM synthesis of PMCs via the circPhf21a-Vegfa axis, which may provide novel therapeutic targets for OA treatment. Video abstract.


Assuntos
Osteoartrite , Fator de Crescimento Transformador beta1 , Animais , Proliferação de Células , Condrócitos/metabolismo , Matriz Extracelular/metabolismo , Camundongos , Osteoartrite/metabolismo , RNA Circular/genética , Fator de Crescimento Transformador beta1/metabolismo , Fator de Crescimento Transformador beta1/farmacologia
15.
Front Chem ; 10: 905563, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572111

RESUMO

The sulfate anion (SO4 2-) is known as an end metabolite of cysteine and methionine, and its proper concentration is associated with the expression of key functions in the physiological system. Thus, maintaining sulfate concentration at a precise level is of great significance for biology, environments, and industrial productions. Fundamental research for sulfate anion chemistry can help understand sulfate-associated physiological processes and related applications, for example, remediation. In this minireview, we summarized recent research progresses in sulfate recognition and separation using crystallization and liquid-liquid extraction. We focused on the studies wherein molecular recognition is the key element and is considered the driving force for selective sulfate separations from aqueous solution.

16.
Angew Chem Int Ed Engl ; 61(22): e202201793, 2022 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-35313060

RESUMO

The fabrication of anion-coordinated assemblies into functional soft materials remains a major challenge. To this end, four C2 -symmetric anion-binding ligands equipped with ortho-phenylene-bridged bis(urea) and amine or amide ends were designed, which generated A2 L3 triple helical architectures upon self-assembly with phosphate ions. Hierarchical intermolecular hydrogen bonds among the terminal amine/amide groups and urea moieties resulted in the formation of functional gels. The obtained gels were further applied for conductive adhesion between different surfaces, displaying excellent flexibility and selective wettability. The viscoelastic gels constructed from anion-coordinated assemblies described in this work represent the first example of a new class of anion-coordination-driven smart materials.


Assuntos
Adesivos , Amidas , Amidas/química , Aminas , Ânions/química , Géis/química , Modelos Moleculares , Ureia/química
17.
Microbes Infect ; 24(3): 104904, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35331909

RESUMO

BACKGROUND: Nonpuerperal mastitis (NPM) causes considerable psychological distress in females, since it is difficult to diagnose and treat. A spectrum of etiological factors can lead to NPM. However, the pathogenesis of NPM remains unclear. Here, we aimed to dissect the role of host gene-microbe interactions in NPM. METHODS: We compared the breast tissue microbiome between NPM patients and controls using 16S rRNA sequencing. We also compared the gut microbiome between NPM patients and healthy controls. Moreover, we investigated whether the breast tissue microbiome was associated with an altered gut microbiome in patients with NPM. We analyzed differentially expressed genes in inflammatory tissues of mammary gland from patients with NPM and normal mammary gland tissues from patients with benign and non-infectious breast disease by RNA-sequencing (RNA-seq). Lastly, we explored the association of specific bacterial taxa with differential expression of immune-related genes and differences in infiltrating immune cells. RESULTS: The breast tissue microbiome from NPM and controls showed significant differences in community composition. The breast tissue shared a relatively small proportion of bacterial communities with the gut in patients with NPM. Ruminococcus (family Ruminococcaceae) of breast tissue was positively correlated with the differentially expression of immune-related genes between NPM patients and controls, including antigen processing and presentation genes (ICAM1, LGMN, THBS1, TAP1, HSPA1B and HSPA1A), cytokine receptor gene IL15RA, and chemokine gene CCN1. Rhizobium of breast tissue was negatively correlated with the differentially expression of the antigen processing and presentation gene HSPA6 between NPM patients and controls. We also found that Ruminococcus (family Ruminococcaceae), Coprococcus, and Clostridium of breast tissue positively correlated with the difference of CD8+ T cells between NPM patients and controls. CONCLUSIONS: We preliminarily explored the potential role of host-microbe interactions in NPM. We demonstrate cross-talk between the breast tissue microbiome and the gut microbiome in patients with NPM. We suggest that NPM microbiome composition influences the immune microenvironment of the disease by affecting the transcriptome. This is an exploratory study and further investigation of host-microbe interactions and its potential mechanism in NPM development are warranted.


Assuntos
Microbioma Gastrointestinal , Mastite , Microbiota , Bactérias , Linfócitos T CD8-Positivos , Feminino , Microbioma Gastrointestinal/genética , Humanos , RNA Ribossômico 16S/genética
18.
Angew Chem Int Ed Engl ; 61(23): e202201789, 2022 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-35293665

RESUMO

An azobenzene-functionalized bis-bis(urea) ligand (Lazo ) and phosphate anion assemble to give the first photoactive "aniono" constructs, tetrahedron (A4 L6 ) and helicate (A2 L3 ), which readily undergo interconversion through cis-trans isomerization of the azo group under irradiation/heating. Most strikingly, the tetrahedral cage can accommodate an [18]crown-6 molecule, which can capture two tetramethylammonium (TMA+ ) ions with an unusually high affinity, even capable of replacing K+ in [K([18]crown-6)]+ to form a {(TMA)2 ⊂ ([18]crown-6)} ⊂ cage "Russian doll" structure. Thus, the current work may provide a model for the light-driven binding and exchange of the biologically important K+ and TMA+ ions.


Assuntos
Compostos de Amônio Quaternário , Ureia , Ânions , Potássio
19.
Biology (Basel) ; 11(3)2022 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-35336826

RESUMO

Paramyosin is an important myofibrillar protein in molluscan smooth muscle. The full-length cDNA encoding paramyosin has been identified from Yesso scallop Patinopecten yessoensis. The length of paramyosin molecule has been found to be 3715 bp, which contains an open reading frame (ORF) of 2805 bp for 934 amino acid residues. Characterization of P. yessoensis paramyosin reveals the typical structural feature of coiled-coil protein, including six α-helix (α1-α6) and one coil (η) structures. Multiple phosphorylation sites have been predicted at the N-terminus of paramyosin, representing the unique phosphorylation sites in scallops. The highest levels of mRNA and protein expression of paramyosin have been found in foot and the smooth adductor muscle. According to whole-mount in situ hybridization (WISH), strong paramyosin mRNA signals were detected in the symmetric positions of anterior and posterior adductor muscles at late larval stages. These findings support that paramyosin may serve as the most important components for myogenesis and catch regulation in scallops. The present findings will not only help uncover the potential function of myofibrillar proteins in molluscs but also provide molecular evidence to infer evolutionary relationships among invertebrates.

20.
Ann Transl Med ; 10(4): 179, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35280410

RESUMO

Background: Diabetic foot ulcer (DFU) is the main cause of disability in diabetic patients. However, the molecular changes underlying the occurrence and progression of DFU remain unclear. We conducted this study to examine gene alterations in different DFU patients. Methods: GSE143735 and GSE134431 transcriptome data sets were acquired from the Gene Expression Omnibus database, and differential expression analyses of the genes in these data sets were performed. A functional enrichment analysis of the differentially expressed genes (DEGs) was performed using clusterProfiler package in R. To examine the correlations between DEGs and significant immune-related genes, we identified the intersecting ulcer-related DEGs, healing-related DEGs, and immune-related DEGs. Finally, we further investigate the relationship between the selected genes with immune cell regulation via a single-sample gene set enrichment analysis, and the infiltration of 28 immune cells in common diabetes samples, unhealed DFU samples, and healed samples DFU were compared. Results: We found 238 upregulated genes and 207 downregulated genes in the diabetic foot (DF) patients with ulcers compared to the DF patients without ulcers, and 74 upregulated genes and 28 downregulated genes in the healed samples compared to the unhealed samples. To examine the main biological functions, we conducted a functional enrichment analysis. The results showed that the biological functions of functional enrichment analysis included neutrophil degranulation, leukocyte chemotaxis, myeloid leukocyte migration, phagosome, cytokine-cytokine receptor interaction, and the chemokine signaling pathway. Interleukin (IL)-1B was more highly expressed in patients with ulcers and healed DFU patients than those without ulcers and unhealed DFU patients. Finally, the immune cell abundance difference results showed that activated cluster of differentiation (CD)8 T cells, central memory CD8 T cells, T follicular helper cells, myeloid-derived suppressor cells, natural killer T cells and monocytes were more highly infiltrated in normal diabetes patients and healed DFU patients than unhealed DFU patients. However, no difference was found between DF patients with and without ulcers. Conclusions: IL-1B is an inflammation gene that can be used to assess and regulate DFU progression.

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