Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.006
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-35511838

RESUMO

Atrial fibrillation (AF) has been proven highly correlated to stroke; more than 43 million people suffer from AF worldwide. However, most of these patients are unaware of their disease. There is no convenient tool by which to conduct a comprehensive screening to identify asymptomatic AF patients. Hence, we provide a non-contact AF detection approach based on remote photoplethysmography (rPPG). We address motion disturbance, the most challenging issue in rPPG technology, with the NR-Net, ATT-Net, and SQ-Mask modules. NR-Net is designed to eliminate motion noise with a CNN model, and ATT-Net and SQ-Mask utilize channel-wise and temporal attention to reduce the influence of poor signal segments. Moreover, we present an AF dataset collected from hospital wards which contains 452 subjects (mean age, 69.313.0 years; women, 46%) and 7,306 30-second segments to verify the proposed algorithm. To our best knowledge, this dataset has the most participants and covers the full age range of possible AF patients. The proposed method yields accuracy, sensitivity, and specificity of 95.69%, 96.76%, and 94.33%, respectively, when discriminating AF from normal sinus rhythm. More than previous studies, other arrhythmias are also taken into consideration, leading to a further investigation of AF vs. Non-AF and AF vs. Other scenarios. For the three scenarios, the proposed approach outperforms the benchmark algorithms. Additionally, the accuracy of the slight motion data improves to 95.82%, 92.39%, and 89.18% for the three scenarios, respectively, while that of full motion data increases by over 3%.

2.
Indian J Ophthalmol ; 70(5): 1837-1840, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35502089

RESUMO

A 49-year-old Indian male presented with rapidly progressive vision loss 1 day after receiving the second dose of BNT162b2 mRNA coronavirus disease 2019 (COVID-19) vaccine (Pfizer-BioNTech, NY, USA). The eye had secondary angle closure glaucoma, bullous retinal detachment, and massive intraocular hemorrhage. Ultrasound showed an ill-defined subretinal mass with moderate internal reflectivity. Magnetic resonance imaging (MRI) confirmed an enhancing heterogeneous subretinal mass. Histopathology showed a necrotic melanocytic lesion arising from the posterior edge of the ciliary body and choroid. Necrotic uveal melanoma was confirmed after expert histopathology opinion. Uveal melanomas can rarely present with tumor necrosis following mRNA COVID-19 vaccination.


Assuntos
COVID-19 , Melanoma , Vacinas contra COVID-19/efeitos adversos , Humanos , Masculino , Melanoma/diagnóstico , Melanoma/patologia , Pessoa de Meia-Idade , Necrose , RNA Mensageiro , Neoplasias Uveais , Vacinação
3.
ACS Macro Lett ; 11(3): 323-328, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-35575374

RESUMO

Statistical copolypeptides comprising lysine and tyrosine with unprecedented ion-induced gelation behavior are reported. Copolypeptides are obtained by one-step N-carboxyanhydride (NCA) ring-opening polymerization. The gelation mechanism is studied by in situ SAXS analyses, in addition to optical spectroscopy and transmission electron microscopy (TEM). It is found that the gelation of these statistically polymerized polypeptides is due to the formation of stable intermolecular ß-sheet secondary structures induced by the presence of salt ions as well as the aggregation of an α-helix between the copolypeptides. This behavior is unique to the statistical lysine/tyrosine copolypeptides and was not observed in any other amino acid combination or arrangement. Furthermore, the diffusion and mechanical properties of these hydrogels can be tuned through tailoring the polypeptide chain length and ion strength.

4.
Ying Yong Sheng Tai Xue Bao ; 33(4): 915-921, 2022 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-35543042

RESUMO

The coKriging method of selecting effective variables is helpful to improve the spatial prediction accuracy of soil available potassium in county-scale rubber plantation, which is of significance in precision fertilization management of rubber plantation. In this study, we analyzed the spatial variability characteristics of soil available potassium in 0-20 cm layer in the rubber plantation of Baisha County, Hainan Province, by geostatistics. The significant characteristic variables were screened by correlation analysis, and the spatial interpolation precisions of coKriging (COK) of different variables were compared. The results showed that the average soil available potassium content in the study area was 44.65 g·kg-1, generally at a state of shortage. The variable coefficient was 52.6%, which was a moderate intensity of variation. The nugget effect was 12.5%, with a strong spatial autocorrelation. The organic matter and elevation were closely related to soil available potassium content. The COK spatial interpolation prediction precisions of the three covariates of organic matter (COK1), elevation (COK2), and organic matter+elevation (COK3) were all higher than ordinary Kriging (OK), and the fitting precision of the cross-validation model was COK1>COK3>COK2>OK. The fitting precision was not proportional to the number of covariates selected. Selecting more correlated covariates was more conducive to reflecting the spatial heterogeneity of soil properties. The soil available potassium content was higher in the northwest and lower in the central and eastern regions, which provided a theoretical basis for the further development of soil potassium management in rubber plantations.

5.
Spectrochim Acta A Mol Biomol Spectrosc ; 278: 121318, 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35525179

RESUMO

This study proposes a method for the rapid identification of elements in soil heavy metal pollution using spectral indices. Set up a simulation experiment of planting crops in soil polluted by multi-gradient Cu and Pb. The obtained polluted soil spectral data was initially pre-processed to obtain the original spectrum (OR), the continuum removed spectrum (CR), and the first-order differential spectrum (FOD). Then the preliminary model of soil heavy metal pollution index (SHMPI) was constructed. Using the correlation optimal algorithm, the maximum median distance algorithm, and the maximum average distance algorithm to select the optimal bands corresponding to the OR, CR, and FOD. The optimal bands selected by each algorithm were substituted into the SHMPI. Each algorithm obtains three indices, and two of them were selected as the x-axis and y-axis to form a two-dimensional pollution identification plane. Nine two-dimensional planes can be obtained by three algorithms and three combinations of OR, CR, and FOD. Support vector machine classifier was used to classify the Cu and Pb polluted samples in the planes, and nine classification models to distinguish Cu and Pb pollution in soil were constructed. The results show that using the correlation optimal algorithm to extract the optimal bands, and using OR and CR to construct SHMPI, the accuracy of the classification line model of Cu and Pb pollution obtained was 93% in the training group and 86% in the validation group. This method can stably and effectively identify the types of heavy metal pollution in soil, and can also effectively identify whether the soil is polluted by heavy metals, which is expected to guide the rapid and non-destructive identification of heavy metal pollution in polluted areas, and provide new ideas for the identification of other types of heavy metals in soil.

6.
Water Res ; 216: 118336, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35378451

RESUMO

The presence of dissolved organic nitrogen (DON) in biological nutrient removal (BNR) effluent has led to increased concern about its adverse effects on wastewater discharge and reuse applications. Previous studies have demonstrated efficient biological inorganic nitrogen removal in BNR under low dissolved oxygen (DO) conditions; however, information on DON is scarce. This study investigated low-DO effects on DON and N-nitrosodimethylamine (NDMA) precursor concentrations in BNR effluents. Identical BNR reactors consisting of an external real-time DO intelligent control system were operated at three different DO concentrations (0.3, 1.0, and 4.0 mgO2/L). Surprisingly, significantly higher values of effluent DON (p<0.05, t-test) and NDMA precursors (p<0.01, t-test) were observed at lower DO levels. Ultrahigh-resolution mass spectrometry analysis showed that molecules produced by microbes at low-DO levels exhibited high proteins/amino sugars-like and low normal oxidation state of carbon characteristics, which possibly acted critical roles in NDMA formation. Furthermore, path analysis by partial least-squares path modeling suggested that NDMA formation potential had strong associations with microbe-DON network stability of microbe-DON co-occurrence interactions (r=0.979, p<0.01). These results highlight the necessity of reconsidering the feasibility of BNR systems operating at low-DO concentrations considering the adverse effects of DON on wastewater discharge and reuse applications.


Assuntos
Dimetilnitrosamina , Águas Residuárias , Nitrogênio/análise , Nutrientes/análise , Oxigênio/análise , Águas Residuárias/análise
7.
Bioengineered ; 13(4): 10274-10286, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35435104

RESUMO

The long noncoding RNA growth arrest-specific transcript 5 (GAS5) has been reported to function as a suppressor in many cancers. However, the role and mechanism of lncRNA GAS5 in pituitary neuroendocrine tumors (PitNETs) remain unclear. Here, we found that lncRNA GAS5 and cylindromatosis (CYLD) expression was downregulated in invasive PitNET tissues and was negatively correlated with miR-27a-5p expression. LncRNA GAS5 overexpression inhibited proliferation of PitNETs cell line MMQ and GH3 cells and induced cell apoptosis, simultaneously, inhibited miR-27a-5p expression and increased CYLD expression. Moreover, miR-27a-5p mimic significantly decreased the luciferase activities of lncRNA GAS5 and CYLD luciferase reporter vector and downregulated CYLD expression, while miR-27a-5p inhibitor increased the expression of CYLD in MMQ and GH3 cells. Furthermore, RNA-immunoprecipitation assay revealed the direct binding between lncRNA GAS5 and miR-27a-5p. Additionally, miR-27a-5p mimic or silenced CYLD attenuated the effect of lncRNA GAS5 on MMQ and GH3 cell proliferation. In vivo lncRNA GAS5 overexpression inhibited GH3 cell tumor growth, while miR-27a-5p mimic or silenced CYLD attenuated the effect of lncRNA GAS5 on GH3 cell tumor growth. These results suggest that lncRNA GAS5 acts as an endogenous sponge by binding miR-27a-5p to increase the expression of its target gene CYLD, thereby inhibits PitNETs cell proliferation and tumor growth.


Assuntos
MicroRNAs , Tumores Neuroendócrinos , RNA Longo não Codificante , Animais , Apoptose/genética , Proliferação de Células/genética , Humanos , Luciferases , MicroRNAs/genética , MicroRNAs/metabolismo , Tumores Neuroendócrinos/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA Nucleolar Pequeno , Ratos
8.
Int J Ophthalmol ; 15(4): 554-559, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35450169

RESUMO

AIM: To investigate the effect of leptin on the angiogenesis of RF/6A cells (monkey retinal choroidal endothelial cells) in vitro and test the cellular signaling in the mechanism. METHODS: RF/6A cells were cultured in vitro and randomly divided into four groups: normal control, with leptin at 50, 100, 200 ng/mL for cell counting kit-8 (CCK8). RF/6A cell proliferation and migration were examined by Transwell assays, while RF/6A cell tube formation by Matrigel assay. JAK2, p-JAK2, STAT3, and p-STAT3 protein expression was measured by Western blotting. Cells were then divided into the following treatment groups: control, 100 ng/mL leptin and AG-490 (100 ng/mL leptin+10 µmol/L AG-490) for examinations of RF/6A cellular behaviour again. Analysis of differences was carried out using one-way ANOVA and least significant difference (LSD). RESULTS: RF/6A cell proliferation, migration and cell tube formation were promoted significantly by leptin in a dose-dependent manner (P<0.05). Western blotting showed that leptin up-regulated p-JAK2 and p-STAT3 expression levels. Treatment with the JAK/STAT pathway inhibitor, AG-490, decreased leptin-induced p-JAK2 and p-STAT3 expression, and inhibited cell proliferation, migration and cell tube formation induced by leptin (P<0.05). CONCLUSION: Leptin can promote RF/6A cell angiogenesis in vitro via activation of the JAK2/STAT3 signaling pathway.

9.
Sci Total Environ ; 833: 155248, 2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35427614

RESUMO

In this study, two lava stone biocarrier facilitated gravity-driven membrane (GDM) reactors were operated at ~8 °C and ~22 °C in parallel for treating primary wastewater effluent. Although the biocarrier reactor at 8 °C displayed less efficient removals of biodegradable organics than that at 22 °C, both GDM systems (without cleaning) showed comparable fouling resistance distribution patterns, accompanying with similar cake filtration constants and pore constriction constants by modelling simulation. Compared to the GDM at 8 °C, more foulants were accumulated on the GDM at 22 °C, but they presented similar soluble organics/inorganics contents and specific cake resistances. This indicated the cake layers at 22 °C may contain greater-sized foulants due to proliferation of both prokaryotes and eukaryotes, leading to a relatively less-porous nature. In the presence of periodic cleaning (at 50 °C), the cleaning effectiveness followed a sequence as ultrasonication-enhanced physical cleaning > two-phase flow cleaning > chemical-enhanced physical cleaning > physical cleaning, regardless of GDM operation temperature. However, significantly higher cake resistances were observed in the GDM system at 22 °C than those at 8 °C, because shear force tended to remove loosely-attached foulant layers and may compress the residual dense cake layer. The presence of periodic cleaning led to dissimilar dominant prokaryotic and eukaryotic communities in the cake layers as those without cleaning and in the lava stone biocarriers. Nevertheless, operation temperature did not influence GDM permeate quality, which met EU discharge standards.

10.
Sci Total Environ ; 833: 155198, 2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35427627

RESUMO

Microplastics (MPs) have been detected in drinking water, seafood, and commodities relevant to human daily life, causing widespread concern. Although there have been studies on the health risks of MPs to mammals, the impact of MPs on populations with intestinal immune imbalance has been greatly ignored. The vulnerability of the body with intestinal immune imbalance may increase the likelihood of its response to MPs, which is in urgent need of relevant research. Here, we compared the effects of 500 µg/L polystyrene microplastics (PSMPs) on healthy mice and mice with intestinal immune imbalance through colon photographs, histopathological analysis, expression of inflammatory cytokines, PSMPs distribution, microbial community analysis, and metabolomics analysis. The results demonstrated that intestinal immune imbalance aggravated the colonic response to PSMPs. PSMPs exposure significantly increased the expression of inflammation factors (TNF-α, IL-1ß and IFN-γ) in mice with intestinal immune imbalance. In addition, the exposure of PSMPs aggravated the histopathological damage of colonic mucosa in mice with intestinal immune imbalance, and exerted great disturbance on the colonic microbial community and metabolism. This may be due to the significant increase of PSMPs accumulation owing to the damage of intestinal barrier in mice with intestinal immune imbalance. In addition, the increase of several pathogenic bacteria including Bacteroides caused by intestinal immune imbalance also increased the toxicity of PSMPs. Our results highlight that individual with intestinal immune imbalance could be more sensitive to environmental pollution, which should be considered during health risk assessment.

11.
Materials (Basel) ; 15(8)2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35454520

RESUMO

Near-infrared spectroscopy has been widely applied in various fields such as food analysis and agricultural testing. However, the conventional method of scanning the full spectrum of the sample and then invoking the model to analyze and predict results has a large amount of collected data, redundant information, slow acquisition speed, and high model complexity. This paper proposes a feature wavelength selection approach based on acousto-optical tunable filter (AOTF) spectroscopy and automatic machine learning (AutoML). Based on the programmable selection of sub nm center wavelengths achieved by the AOTF, it is capable of rapid acquisition of combinations of feature wavelengths of samples selected using AutoML algorithms, enabling the rapid output of target substance detection results in the field. The experimental setup was designed and application validation experiments were carried out to verify that the method could significantly reduce the number of NIR sampling points, increase the sampling speed, and improve the accuracy and predictability of NIR data models while simplifying the modelling process and broadening the application scenarios.

12.
J Am Chem Soc ; 144(14): 6543-6550, 2022 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-35378033

RESUMO

Fluorinated motifs are frequently encountered in drugs and agrochemicals. Incorporating fluorine-containing motifs in drug candidates for lead optimization in pharmaceutical research and development has emerged as a powerful tool. The construction of molecules that feature a trifluoromethyl (CF3-) group on a stereogenic carbon has accumulated broad research efforts. Unlike its well-explored, biologically active methyl counterpart, asymmetric construction of ß-trifluoromethylated alcohols bearing adjacent stereocenters still remains elusive. Through retrosynthetic analysis, we posited that followed by sequential reduction of carbonyl, the initial construction of chiral α-trifluoromethylated ketones could render the desired product in a facile, one-pot fashion. Herein, we developed the first example of nickel-catalyzed asymmtric reductive cross-coupling trifluoroalkylation of acyl chlorides for enantioselective synthesis of diverse α-trifluoromethylated ketones. The one-pot reduction of these α-trifluoromethylated ketones furnished corresponding alcohols bearing ß-CF3-substituted stereogenic carbons with excellent diastereoselectivity and complete enantioselective retention. High yields/enantioselectivity, mild conditions, and good functional group compatibility are shown in the system. Utilities of the method are also illustrated by applying asymmetric, late-stage trifluoroalkylation of biologically active complex molecules, revealing tremendous potential for development of CF3-containing chiral drugs.


Assuntos
Álcoois , Cetonas , Carbono/química , Catálise , Cetonas/química , Níquel , Estereoisomerismo
13.
Dis Markers ; 2022: 7550090, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35251376

RESUMO

OBJECTIVE: The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hordeolum. METHODS: This was a prospective, matched-pair study. A total of eight patients with internal hordeolum who attended the ophthalmology clinic of our hospital from April to August 2020 were included. Meibomian gland secretions were collected from subjects before treatment (Group A) and from patients cured after eyelid cleaning with hypochlorous acid eyelid wipes for 7 days (Group B). Samples were submitted to 16S rRNA high-throughput sequencing, and the resulting data were analyzed to compare the differences in the structure and composition of meibomian gland secretion microbial flora before and after treatment of internal hordeolum. RESULTS: A total of 2127 operational taxonomic units were obtained from the two groups of samples, and there was no significant difference in alpha diversity before and after eyelid cleaning. At the phylum level, there was no significant difference between the two groups. The predominant phyla in Group A included the following: Firmicutes (32.78% ± 20.16%), Proteobacteria (26.73% ± 7.49%), Acidobacteria (10.58% ± 11.45%), Bacteroidetes (9.05% ± 6.63%), Actinobacteria (8.48% ±1.77%), and Chloroflexi (3.15% ± 3.12%), while those in Group B were the following: Proteobacteria (31.86% ± 9.69%), Firmicutes (29.07% ± 24.20%), Acidobacteria (11.33% ± 7.53%), Actinobacteria (7.10% ± 1.98%), Bacteroidetes (5.39% ± 5.17%), and Chloroflexi (3.89% ± 3.67%). Starting from the class level, significant differences in microbial communities were detected before and after eyelid cleaning (P < 0.05). Linear discriminant analysis effect size analysis showed the core flora in Group A microbiome comprising Actinobacteria, Staphylococcus, Staphylococcaceae, Staphylococcus aureus, Ruminococcacea UCg-014, Ruminococcacea-UCG-014, Halomonadaceae, Neisseria, Methylobacterium, Frankiales, and Neisseria sicca, while those in Group B microbial were Streptococcus sp., Blautia, Bifidobacterium pseudocatenulatum, Subdoligranulum, Subdoligranulum variabile, Faecalibacterium, and Faecalibacterium prausnitzii. CONCLUSION: Eyelid cleaning with hypochlorous acid eyelid wipes does not change the biodiversity in the meibomian gland secretions of patients with internal hordeolum. Hypochlorous acid eyelid wipes may affect the internal hordeolum through broad-spectrum antibacterial action to effectively reduce the relative abundance of symbiotic pathogens, such as Staphylococcus, Neisseria, Actinomycetes, and Ruminococcus and increase that of Faecalibacterium prausnitzii and other symbiotic probiotics with anti-inflammatory effects.


Assuntos
Bactérias/genética , Terçol/tratamento farmacológico , Ácido Hipocloroso/uso terapêutico , Glândulas Tarsais/microbiologia , Microbiota , Oxidantes/uso terapêutico , Adulto , Biodiversidade , Feminino , Humanos , Masculino , Estudos Prospectivos , RNA Ribossômico 16S/genética
15.
BMC Musculoskelet Disord ; 23(1): 300, 2022 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-35351065

RESUMO

STUDY DESIGN: Retrospective case-control radiographic study. OBJECTIVE: To identify main effects of preoperative pattern and global coronal malalignment (GCM) on postoperative coronal imbalance in degenerative lumbar scoliosis (DLS) patients and evaluate the rationality of the classification of coronal deformity based on preoperative GCM. A classification of coronal deformity based on preoperative GCM (20 mm set as the threshold of coronal imbalance) has been proposed recently, but whether it is practical is unclear. METHODS: One hundred twelve DLS patients treated with posterior instrumented fusion were reviewed. Coronal measurements included GCM and major Cobb angle. Based on relationship between C7 PL and major curve, preoperative patterns were classified into: Pattern 1(concave pattern), C7 PL shifted to the concave side of major curve; Pattern 2(convex pattern), C7 PL shifted to the convex side of major curve. Patients were separated into 4 groups (3 types): Type 0-1: GCM < 20 mm plus Pattern 1; Type 0-2: GCM < 20 mm plus Pattern 2; Type 1: GCM > 20 mm plus Pattern 1; Type 2: GCM > 20 mm plus Pattern 2. After comparison within patterns or among 4 groups, further factorial analysis was performed. RESULTS: Significant differences regarding postoperative GCM or coronal imbalance/balance ratio existed among 4 groups (F = 6.219, p = 0.001; x2 = 22.506, p < 0.001, respectively), despite no significant difference in intra-pattern 1(concave pattern) or intra-pattern 2(convex pattern) groups. Two-way analysis of variance showed preoperative pattern exhibited significant effect on postoperative GCM or imbalance/balance ratio (F(1,108) = 14.286, p < 0.001; F(1,108) = 30.514, p < 0.001, respectively) while neither preoperative GCM alone nor interaction of preoperative GCM with pattern did. CONCLUSION: In DLS patients, it's the preoperative pattern other than GCM that had main effects on postoperative coronal imbalance. Classification of coronal deformity based on preoperative GCM is questionable.


Assuntos
Escoliose , Fusão Vertebral , Adulto , Humanos , Região Lombossacral , Período Pós-Operatório , Estudos Retrospectivos , Escoliose/diagnóstico por imagem , Escoliose/etiologia , Escoliose/cirurgia , Fusão Vertebral/efeitos adversos
16.
JAMA Oncol ; 2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-35323856

RESUMO

Importance: Induction chemotherapy added to concurrent chemoradiotherapy significantly improves survival for patients with locoregionally advanced nasopharyngeal carcinoma, but the optimal induction regimen remains unclear. Objective: To determine whether induction chemotherapy with paclitaxel, cisplatin, and capecitabine (TPC) improves survival vs cisplatin and fluorouracil (PF) prior to chemoradiotherapy for patients with stage IVA to IVB nasopharyngeal carcinoma. Design, Setting, and Participants: This randomized, open-label, phase 3 clinical trial recruited 238 patients at 4 hospitals in China from October 20, 2016, to August 29, 2019. Patients were 18 to 65 years of age with treatment-naive, nonkeratinizing stage IVA to IVB nasopharyngeal carcinoma and an Eastern Cooperative Oncology Group performance status of 0 to 1. Interventions: Patients were randomly assigned (1:1) to receive induction chemotherapy with two 21-day cycles of TPC (intravenous paclitaxel [150 mg/m2, day 1], intravenous cisplatin [60 mg/m2, day 1], and oral capecitabine [1000 mg/m2 orally twice daily, days 1-14]) or PF (intravenous cisplatin [100 mg/m2, day 1] and fluorouracil [800 mg/m2 daily, days 1-5]), followed by chemoradiotherapy. Main Outcomes and Measures: The primary end point was failure-free survival in the intention-to-treat population. Secondary end points included distant metastasis-free survival, locoregional relapse-free survival, overall survival, tumor response, and safety. Results: Overall, 238 eligible patients (187 men [78.6%]; median age, 45 years [range, 18-65 years]) were randomly assigned to receive TPC (n = 118) or PF (n = 120). The median follow-up duration was 48.4 months (IQR, 39.6-53.3 months). Failure-free survival at 3 years was 83.5% (95% CI, 77.0%-90.6%) in the TPC group and 68.9% (95% CI, 61.1%-77.8%) in the PF group (stratified hazard ratio [HR] for recurrence or death, 0.47; 95% CI, 0.28-0.79; P = .004). Induction with the TPC regimen resulted in a significant reduction in the risk of distant metastases (stratified HR, 0.49 [95% CI, 0.24-0.98]; P = .04) and locoregional recurrence (stratified HR, 0.40 [95% CI, 0.18-0.93]; P = .03) compared with the PF regimen. However, there was no effect on early overall survival (stratified HR, 0.45 [95% CI, 0.17-1.18]; P = .10). The incidences of grade 3 to 4 acute adverse events and late-onset toxicities were 57.6% (n = 68) and 13.6% (16 of 118), respectively, in the TPC group and 65.8% (n = 79) and 17.9% (21 of 117), respectively, in the PF group. One treatment-related death occurred in the PF group. Conclusions and Relevance: This randomized clinical trial found that induction chemotherapy with 2 cycles of TPC for patients with stage IVA to IVB nasopharyngeal carcinoma improved failure-free survival compared with 2 cycles of PF, with no increase in the toxicity profile. Trial Registration: ClinicalTrials.gov Identifier: NCT02940925.

17.
Nanoscale ; 14(14): 5412-5424, 2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35319556

RESUMO

Two-dimensional (2D) van der Waals (vdW) materials with tunable heterostructures and superior optoelectronic properties have opened a new platform for various applications, e.g., field-effect transistors, ultrasensitive photodetectors and photocatalysts. In this work, an InSe/InSe(Ge) (germanium doped InSe) vdW heterostructure is designed to improve the photoresponse performance of sole InSe in a photoelectrochemical (PEC)-type photodetector. Photoelectrochemical measurements demonstrated that this heterostructure has excellent photoresponse characteristics, including a photocurrent density of 9.8 µA cm-2, a photo-responsivity of 64 µA W-1, and a response time/recovery time of 0.128 s/0.1 s. Moreover, the measurements also revealed the self-powering capability and long-term cycling stability of this heterostructure. The electronic properties of the prepared pure and Ge-doped single crystals unveiled a negative and temperature-independent thermoelectric power and temperature-activated resistivity. The negative character of dominating charge carriers was confirmed by Hall measurements, which corroborated by electrical resistivity revealed a carrier concentration below ∼1015 cm-3 and an electron mobility of ∼500 cm2 V-1 s-1 in Ge-doped crystals. Additionally, the Mott-Schottky model explored the mechanism of charge transfer and enhanced PEC performance. Band bending at the InSe/InSe(Ge)-electrolyte interface benefits the separation and transformation of photogenerated carriers from the heterostructure to electrolyte due to the tunable energy band alignment. These results indicate that the InSe/InSe(Ge) vdW heterostructure is promising for PEC-type photodetectors, which provide a novel way to utilize 2D vdW heterostructures in optoelectronics.

18.
Biochem Pharmacol ; 198: 114952, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35149050

RESUMO

PURPOSE: Allergic asthma is a chronic inflammatory disorder with airway hyperresponsiveness and tissue remodeling as the main pathological characteristics. The etiology of asthma is relatively complicated, involving genetic susceptibility, epigenetic regulation, environmental factors, and immune imbalance. Colony stimulating factor 1 receptor (CSF-1R), highly expressed in myeloid monocytes, plays an important role in regulating inflammation. However, the pathological role of CSF-1R and the therapeutic effects of CSF-1R inhibitor in allergic airway inflammation remain indistinct. METHODS: The house dust mite (HDM)-triggered allergic airway inflammation model was conducted to fully uncover the efficacies of CSF-1R inhibition, as illustrated by histopathological examinations, biochemical analysis, ELISA, RT-PCR, Western blotting assay, immunofluorescence, and flow cytometry. Furthermore, bone marrow-derived macrophages (BMDMs) were differentiated and polarized upon IL-4/IL-13 induction to clarify the underlying mechanisms of CSF-1R inhibition. RESULTS: Herein, we presented that the expression of CSF-1R was increased in HDM-induced experimental asthma and inhibition of CSF-1R displayed dramatic effects on the disease severity of asthma, referring to suppressing the secretion of allergic mediators, dysfunction of airway epithelium, and infiltration of inflammatory cells. Furthermore, CSF-1R inhibitor could markedly restrain the polarization and expression of transcriptional factors of alternatively activated macrophages (AAMs) in the presence of IL-4/IL-13 and reduce the recruitment of CSF-1R-dominant macrophages, both in acute and chronic allergic airway inflammation model. CONCLUSION: Collectively, our findings demonstrated the molecular pathological mechanism of CSF-1R in allergic airway diseases and suggested that targeting CSF-1R might be an alternative intervention strategy on the homeostasis of airway immune microenvironment in asthma.


Assuntos
Asma , Hipersensibilidade , Animais , Asma/metabolismo , Modelos Animais de Doenças , Epigênese Genética , Hipersensibilidade/metabolismo , Inflamação , Interleucina-13/metabolismo , Interleucina-4/metabolismo , Pulmão/metabolismo , Macrófagos/metabolismo , Pyroglyphidae
19.
Chin J Traumatol ; 25(2): 83-89, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35177289

RESUMO

PURPOSE: To analyze the curative effect and technical points of a modified posteromedial approach in the treatment of Klammer III posterior Pilon fracture. METHODS: A retrospective analysis of patients with Klammer III posterior Pilon fractures were conducted in our department from January 2018 to December 2019. Before the surgery, the patients were fully relieved of swelling and pain, and a comprehensive examination was carried out. The posteromedial approach exposed the posterior and medial fracture block of the distal tibia. According to the fracture of external malleolus, it is determined whether to combine a lateral incision and protect tendons and vascular nerves by a retractor, and then perform a fracture reduction and internal fixation. Postoperatively, the patients were treated with analgesia, detumescence, anticoagulation and rehabilitation exercise. The American orthopaedic foot and ankle society (AOFAS) score and visual analogue score were recorded at regular follow-up after surgery. A t-test was used for the comparison of the preoperative and final AOFAS score. RESULTS: There were 7 male and 13 female (n = 20) included in the study, aged 22 to 88 years (average age 54.2 years). The injury mechanisms were falling from a height (n = 7), traffic accident (n = 6), walking injury (n = 2) and heavy injury (n = 5). The postoperative follow-up duration was 12-24 months (mean 16.95 months). The AOFAS score of the 20 patients before and after surgery were compared. The preoperative AOFAS score was 38.90 ± 3.91, and the final AOFAS score was 80.55 ± 4.20, (p < 0.001). The mean final visual analogue scores at rest, active and weight-bearing walking were 0.30, 0.85 and 1.70, respectively. One patient reported poor postoperative wound healing and required a return to hospital for debridement and anti-infection treatment. CONCLUSION: In the treatment of Klammer III posterior Pilon fractures, the modified posteromedial approach can fully expose the fracture block and the collapsed articular surface of the medial malleolus, achieve good reduction and internal fixation with limited injury of the tendon and vascular nerves, and have a better prognosis.


Assuntos
Fraturas do Tornozelo , Fraturas da Tíbia , Adulto , Idoso , Idoso de 80 Anos ou mais , Fraturas do Tornozelo/cirurgia , Feminino , Fixação Interna de Fraturas , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fraturas da Tíbia/cirurgia , Resultado do Tratamento , Adulto Jovem
20.
Acta Pharm Sin B ; 12(1): 228-245, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35127382

RESUMO

Phosphodiesterase-4 (PDE4) functions as a catalyzing enzyme targeting hydrolyzation of intracellular cyclic adenosine monophosphate (cAMP) and inhibition of PDE4 has been proven to be a competitive strategy for dermatological and pulmonary inflammation. However, the pathological role of PDE4 and the therapeutic feasibility of PDE4 inhibitors in chronic ulcerative colitis (UC) are less clearly understood. This study introduced apremilast, a breakthrough in discovery of PDE4 inhibitors, to explore the therapeutic capacity in dextran sulfate sodium (DSS)-induced experimental murine chronic UC. In the inflamed tissues, overexpression of PDE4 isoforms and defective cAMP-mediating pathway were firstly identified in chronic UC patients. Therapeutically, inhibition of PDE4 by apremilast modulated cAMP-predominant protein kinase A (PKA)-cAMP-response element binding protein (CREB) signaling and ameliorated the clinical symptoms of chronic UC, as evidenced by improvements on mucosal ulcerations, tissue fibrosis, and inflammatory infiltrations. Consequently, apremilast maintained a normal intestinal physical and chemical barrier function and rebuilt the mucosal homeostasis by interfering with the cross-talk between human epithelial cells and immune cells. Furthermore, we found that apremilast could remap the landscape of gut microbiota and exert regulatory effects on antimicrobial responses and the function of mucus in the gut microenvironment. Taken together, the present study revealed that intervene of PDE4 provided an infusive therapeutic strategy for patients with chronic and relapsing UC.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...